RLG00000036026

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003425
Physical Location & Seq
Forward (+)
75093531 .. 75096428
2898 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036026

Sequence Viewer

Length: 1587 bp
ATGCAGAGTCAGGGTATGGAGGTAGGAGAAGAAGATGATAAAGATGATGATGATTCAGTTCTAGTTTCGAGCCAACTCAGCCAAGTGTTTATGAAGCGCTGCTTCCCACTTCCCCAATTCGCACTAAACCTAATCTTCAATTTCATCCCAAGCCGGCCTAAAGGAATGGCAGAAGTTGGAGAAAGCTCTGGTGCAAAGCCCTCTGGTGAAATCTGGGCCAAGCTTGTTCCTTCCGACTCGAGATTTCCGGATGTTGAGATCAGAGATCTTGTTCTGACTCAAGATACCCCTCAGAGTCAAGATATTGGTTCAGATGAGAGTTTAGTTTGCTCTCCTATTGACAAATGCGAGTGGTGCAGAATTACGAGGAACCCCGATCAGTCTTCTGCCACTATACAGAATAAGAGTTCAAATGCAATACTTGTTGATGGAGCTGCGATCCAATCCGAAGAGAGTACTGTTATCAGATCTGGAAGTGAAATTATTACAGGTCTTCAGAGAGAAGGGTGTTTAAGCTACAGATTCAATGTGTTGCTTGGTCCTGAAACTTGTCAGAAGATGCTGAAGATTAGTGTGGATGCTGAGCATGCAAAGTGCTGCATTTGTTTAAATATATGGCATGATGCTGTTACCATTGCTCCTTGCTTCCATAACTTCTGCAATGGGTGCTTCTCAGAGTGGTTAAGGAGATCACAGGAGAAACGTTCAAGTGTACTCTGTCCCCAATGTCGAGCAGTTGTGCAATTTGTTGGAAGAAACCACTTCCTGCATAACATTGCCGATGAAATACTGAATGCGGATTCTTCACTGAGACGCTCCAACGAAGACACTGCAGTTTTAGATTCCTATGCAACAGTACGATCAAATCTGGTAATAAAGTCGGGAAAAGGGCTGCTTGGGAAAAGGGTTCGTTCAGCAGTGCAAGATGAAGCTGATAGTGTAGAGCTTCCCTGCCCTCAATGCGGTAATCAATTTGGTGGGTTTCTCTGCAACCGAAGCACTACCCATCTGCAATGTCAAGCATGTGGAGGAATGATGCCTTCACGAACCAGTACAAGTGTACCTCAACACTGTATAGGGTGTGATAGGTCATTTTGCGGTGCATATTGGCATGCTCAAGGTGTTGCACAAAGTGCTTCTCACCACATATGCAGTCGCGATACTTTCAAACCAATCTCAGAACGCACCATCTCTAGAATTCCTACTTCAGCACATGAAAACAATCGCCATGAACAAGAAGTCACTATGAGGTGTATTGCACAAATGGGAAGAACATTGCAGGATGTTATTGCAGAATGGATGGTAATGTTCAATAACAGAACAATTGATCGAACTAACAGGATGCCACTGAAGCATGCTGAGCTGATAACTTCTGGGACATATACATGTAGTGATTGTTATGAGAAACTGATCTCATTCCTCTTGTACTGGTTTCGGGTTTCAGTTTCTGCATCTCATCTTCCCCCAGATGCATCAAAAAGGGAAAATTGCTGGTATGGATATGCATGTCGAACACAGCACCATAATGAAGACCATGCTCGTAAAAGGAATCATGTATGCCGGCCGACTAGGGGTGCTAATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000166 GO:0000209 GO:0000278 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016604 GO:0016605 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0022402 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031647 GO:0031648 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0036094 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045732 GO:0045786 GO:0045862 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051726 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0097159 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1903047 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-RING_2

Protein Analysis

529

Amino Acids

59.06

Weight (kDa)

6.65

Isoelectric Point (pI)

55.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 198 - 244 4.1e-07 Ring finger domain
zf-C3HC4_2 PF13923 198 - 243 1.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 199 - 243 3.2e-06 Zinc finger, C3HC4 type (RING finger)
zf-CRD PF17979 318 - 481 6e-48 Cysteine rich domain with multizinc binding regions
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1158
AccIII TCCGGA 1 cut(s) 247
AciI CCGC 3 cut(s) 797, 963, 1098
AclI AACGTT 1 cut(s) 703
AclWI GGATC 1 cut(s) 433
AcoI YGGCCR 1 cut(s) 1562
AcsI RAATTY 1 cut(s) 1197
AcuI CTGAAG 4 cut(s) 479, 584, 1191, 1370
AdeI CACNNNGTG 1 cut(s) 1133
AfaI GTAC 6 cut(s) 457, 714, 858, 1054, 1062, 1427
AfeI AGCGCT 1 cut(s) 98
AfiI CCNNNNNNNGG 2 cut(s) 962, 1571
AflIII ACRYGT 1 cut(s) 1385
AgsI TTSAA 6 cut(s) 139, 411, 526, 708, 1168, 1312
AluBI AGCT 7 cut(s) 186, 223, 434, 516, 932, 946, 1363
AluI AGCT 7 cut(s) 186, 223, 434, 516, 932, 946, 1363
Alw26I GTCTC 1 cut(s) 805
AlwI GGATC 1 cut(s) 433
AlwNI CAGNNNCTG 1 cut(s) 1448
Ama87I CYCGRG 1 cut(s) 238
Aor13HI TCCGGA 1 cut(s) 247
Aor51HI AGCGCT 1 cut(s) 98
AoxI GGCC 3 cut(s) 155, 216, 1562
ApeKI GCWGC 4 cut(s) 99, 434, 597, 892
ApoI RAATTY 1 cut(s) 1197
AspLEI GCGC 1 cut(s) 99
AspS9I GGNCC 2 cut(s) 216, 539
AsuHPI GGTGA 2 cut(s) 218, 1133
AvaI CYCGRG 1 cut(s) 238
AvaII GGWCC 1 cut(s) 539
BaeI ACNNNNGTAYC 2 cut(s) 1044, 1077
BbsI GAAGAC 4 cut(s) 375, 485, 831, 1536
BbvI GCAGC 4 cut(s) 86, 421, 584, 879
BccI CCATC 4 cut(s) 422, 1014, 1196, 1294
BcgI CGANNNNNNTGC 2 cut(s) 812, 846
BcoDI GTCTC 1 cut(s) 805
BfaI CTAG 3 cut(s) 62, 1194, 1569
BfmI CTRYAG 2 cut(s) 517, 831
BfoI RGCGCY 1 cut(s) 100
BglII AGATCT 2 cut(s) 265, 467
BisI GCNGC 4 cut(s) 100, 435, 598, 893
BlpI GCTNAGC 2 cut(s) 582, 1359
BlsI GCNGC 4 cut(s) 101, 436, 599, 894
BmcAI AGTACT 1 cut(s) 457
Bme18I GGWCC 1 cut(s) 539
BmeT110I CYCGRG 1 cut(s) 238
BmgT120I GGNCC 2 cut(s) 216, 539
BmiI GGNNCC 1 cut(s) 371
BmsI GCATC 8 cut(s) 549, 568, 613, 1026, 1332, 1459, 1460, 1481
BpiI GAAGAC 4 cut(s) 375, 485, 831, 1536
Bpu1102I GCTNAGC 2 cut(s) 582, 1359
BpuEI CTTGAG 2 cut(s) 264, 1101
BsaWI WCCGGW 1 cut(s) 247
BsaXI ACNNNNNCTCC 2 cut(s) 622, 652
Bsc4I CCNNNNNNNGG 2 cut(s) 962, 1571
Bse118I RCCGGY 2 cut(s) 153, 1560
Bse1I ACTGG 2 cut(s) 1050, 1433
Bse3DI GCAATG 5 cut(s) 633, 667, 774, 1019, 1274
BseAI TCCGGA 1 cut(s) 247
BseGI GGATG 6 cut(s) 144, 256, 583, 1288, 1305, 1347
BseLI CCNNNNNNNGG 2 cut(s) 962, 1571
BseMI GCAATG 5 cut(s) 633, 667, 774, 1019, 1274
BseMII CTCAG 7 cut(s) 91, 305, 573, 687, 800, 1191, 1350
BseNI ACTGG 2 cut(s) 1050, 1433
BseX3I CGGCCG 1 cut(s) 1562
BseXI GCAGC 4 cut(s) 86, 421, 584, 879
BsgI GTGCAG 1 cut(s) 376
Bsh1236I CGCG 1 cut(s) 1158
Bsh1285I CGRYCG 1 cut(s) 1565
BshFI GGCC 3 cut(s) 157, 218, 1564
BsiEI CGRYCG 1 cut(s) 1565
BsiHKCI CYCGRG 1 cut(s) 238
BsiSI CCGG 3 cut(s) 154, 248, 1561
BslFI GGGAC 2 cut(s) 705, 1390
BslI CCNNNNNNNGG 2 cut(s) 962, 1571
BsmAI GTCTC 1 cut(s) 805
BsmBI CGTCTC 1 cut(s) 805
BsmFI GGGAC 2 cut(s) 705, 1390
BsmI GAATGC 1 cut(s) 799
BsnI GGCC 3 cut(s) 157, 218, 1564
BsoBI CYCGRG 1 cut(s) 238
Bsp13I TCCGGA 1 cut(s) 247
Bsp143I GATC 9 cut(s) 258, 265, 376, 438, 467, 689, 860, 1327, 1410
Bsp1720I GCTNAGC 2 cut(s) 582, 1359
Bsp68I TCGCGA 1 cut(s) 1158
BspACI CCGC 3 cut(s) 797, 963, 1098
BspANI GGCC 3 cut(s) 157, 218, 1564
BspCNI CTCAG 7 cut(s) 90, 304, 574, 686, 801, 1190, 1351
BspEI TCCGGA 1 cut(s) 247
BspFNI CGCG 1 cut(s) 1158
BspLI GGNNCC 1 cut(s) 371
BspMAI CTGCAG 1 cut(s) 835
BspPI GGATC 1 cut(s) 433
BsrDI GCAATG 5 cut(s) 633, 667, 774, 1019, 1274
BsrFI RCCGGY 2 cut(s) 153, 1560
BsrI ACTGG 2 cut(s) 1050, 1433
BssAI RCCGGY 2 cut(s) 153, 1560
BssMI GATC 9 cut(s) 258, 265, 376, 438, 467, 689, 860, 1327, 1410
Bst4CI ACNGT 3 cut(s) 460, 856, 1073
Bst6I CTCTTC 1 cut(s) 444
BstAPI GCANNNNNTGC 2 cut(s) 666, 1133
BstC8I GCNNGC 5 cut(s) 155, 588, 1113, 1356, 1562
BstDEI CTNAG 7 cut(s) 77, 291, 582, 673, 809, 1177, 1359
BstF5I GGATG 6 cut(s) 144, 256, 583, 1288, 1305, 1347
BstFNI CGCG 1 cut(s) 1158
BstH2I RGCGCY 1 cut(s) 100
BstHHI GCGC 1 cut(s) 99
BstKTI GATC 9 cut(s) 261, 268, 379, 441, 470, 692, 863, 1330, 1413
BstMAI GTCTC 1 cut(s) 805
BstMBI GATC 9 cut(s) 258, 265, 376, 438, 467, 689, 860, 1327, 1410
BstMCI CGRYCG 1 cut(s) 1565
BstMWI GCNNNNNNNGC 9 cut(s) 78, 354, 587, 666, 960, 996, 1133, 1351, 1360
BstNSI RCATGY 6 cut(s) 590, 1026, 1115, 1358, 1389, 1509
BstSFI CTRYAG 2 cut(s) 517, 831
BstUI CGCG 1 cut(s) 1158
BstV1I GCAGC 4 cut(s) 86, 421, 584, 879
BstV2I GAAGAC 4 cut(s) 375, 485, 831, 1536
BstX2I RGATCY 2 cut(s) 265, 467
BstYI RGATCY 2 cut(s) 265, 467
BstZI CGGCCG 1 cut(s) 1562
BsuRI GGCC 3 cut(s) 157, 218, 1564
BtsCI GGATG 6 cut(s) 144, 256, 583, 1288, 1305, 1347
BtsI GCAGTG 2 cut(s) 828, 924
BtsIMutI CAGTG 5 cut(s) 806, 828, 924, 1069, 1346
BtuMI TCGCGA 1 cut(s) 1158
Cac8I GCNNGC 5 cut(s) 155, 588, 1113, 1356, 1562
CaiI CAGNNNCTG 1 cut(s) 1448
CfoI GCGC 1 cut(s) 99
Cfr10I RCCGGY 2 cut(s) 153, 1560
Cfr13I GGNCC 2 cut(s) 216, 539
CseI GACGC 1 cut(s) 822
Csp6I GTAC 6 cut(s) 456, 713, 857, 1053, 1061, 1426
CviQI GTAC 6 cut(s) 456, 713, 857, 1053, 1061, 1426
DdeI CTNAG 7 cut(s) 77, 291, 582, 673, 809, 1177, 1359
DpnI GATC 9 cut(s) 260, 267, 378, 440, 469, 691, 862, 1329, 1412
DpnII GATC 9 cut(s) 258, 265, 376, 438, 467, 689, 860, 1327, 1410
DraI TTTAAA 1 cut(s) 609
DraIII CACNNNGTG 1 cut(s) 1133
EaeI YGGCCR 1 cut(s) 1562
EagI CGGCCG 1 cut(s) 1562
Eam1104I CTCTTC 1 cut(s) 444
EarI CTCTTC 1 cut(s) 444
EclXI CGGCCG 1 cut(s) 1562
Eco47I GGWCC 1 cut(s) 539
Eco47III AGCGCT 1 cut(s) 98
Eco52I CGGCCG 1 cut(s) 1562
Eco57I CTGAAG 4 cut(s) 479, 584, 1191, 1370
Eco88I CYCGRG 1 cut(s) 238
EcoRI GAATTC 1 cut(s) 1197
EcoT22I ATGCAT 2 cut(s) 1474, 1507
Esp3I CGTCTC 1 cut(s) 805
FalI AAGNNNNNCTT 4 cut(s) 86, 118, 879, 911
FaqI GGGAC 2 cut(s) 705, 1390
FauNDI CATATG 1 cut(s) 1148
Fnu4HI GCNGC 4 cut(s) 100, 435, 598, 893
FokI GGATG 6 cut(s) 131, 263, 590, 1295, 1312, 1354
Fsp4HI GCNGC 4 cut(s) 100, 435, 598, 893
FspBI CTAG 3 cut(s) 62, 1194, 1569
GlaI GCGC 1 cut(s) 98
GluI GCNGC 4 cut(s) 100, 435, 598, 893
HaeII RGCGCY 1 cut(s) 100
HaeIII GGCC 3 cut(s) 157, 218, 1564
HapII CCGG 3 cut(s) 154, 248, 1561
HgaI GACGC 1 cut(s) 822
HhaI GCGC 1 cut(s) 99
Hin6I GCGC 1 cut(s) 97
HinP1I GCGC 1 cut(s) 97
HindIII AAGCTT 1 cut(s) 221
HinfI GANTC 9 cut(s) 7, 53, 236, 277, 295, 522, 800, 842, 1549
HpaII CCGG 3 cut(s) 154, 248, 1561
HphI GGTGA 2 cut(s) 218, 1133
Hpy166II GTNNAC 2 cut(s) 713, 1061
Hpy8I GTNNAC 2 cut(s) 713, 1061
HpyAV CCTTC 3 cut(s) 240, 497, 1050
HpyCH4III ACNGT 3 cut(s) 460, 856, 1073
HpyCH4IV ACGT 1 cut(s) 703
HpyF10VI GCNNNNNNNGC 9 cut(s) 78, 354, 587, 666, 960, 996, 1133, 1351, 1360
HpyF3I CTNAG 7 cut(s) 77, 291, 582, 673, 809, 1177, 1359
HpySE526I ACGT 1 cut(s) 703
HspAI GCGC 1 cut(s) 97
Kpn2I TCCGGA 1 cut(s) 247
KroI GCCGGC 2 cut(s) 153, 1560
KroNI GCCGGC 2 cut(s) 155, 1562
Kzo9I GATC 9 cut(s) 258, 265, 376, 438, 467, 689, 860, 1327, 1410
LmnI GCTCC 3 cut(s) 431, 643, 821
Lsp1109I GCAGC 4 cut(s) 86, 421, 584, 879
LweI GCATC 8 cut(s) 549, 568, 613, 1026, 1332, 1459, 1460, 1481
MaeI CTAG 3 cut(s) 62, 1194, 1569
MaeII ACGT 1 cut(s) 703
MaeIII GTNAC 2 cut(s) 628, 1240
MalI GATC 9 cut(s) 260, 267, 378, 440, 469, 691, 862, 1329, 1412
MboI GATC 9 cut(s) 258, 265, 376, 438, 467, 689, 860, 1327, 1410
MfeI CAATTG 1 cut(s) 1323
MflI RGATCY 2 cut(s) 265, 467
MluCI AATT 9 cut(s) 116, 139, 360, 480, 743, 971, 1197, 1323, 1486
MlyI GAGTC 4 cut(s) 16, 230, 271, 304
MmeI TCCRAC 4 cut(s) 157, 258, 730, 843
MnlI CCTC 9 cut(s) 13, 211, 300, 360, 966, 1022, 1074, 1242, 1430
Mph1103I ATGCAT 2 cut(s) 1474, 1507
MroI TCCGGA 1 cut(s) 247
MroNI GCCGGC 2 cut(s) 153, 1560
MseI TTAA 3 cut(s) 512, 608, 683
MslI CAYNNNNRTG 2 cut(s) 1384, 1524
MspI CCGG 3 cut(s) 154, 248, 1561
MunI CAATTG 1 cut(s) 1323
Mva1269I GAATGC 1 cut(s) 799
MvnI CGCG 1 cut(s) 1158
MwoI GCNNNNNNNGC 9 cut(s) 78, 354, 587, 666, 960, 996, 1133, 1351, 1360
NaeI GCCGGC 2 cut(s) 155, 1562
NdeI CATATG 1 cut(s) 1148
NdeII GATC 9 cut(s) 258, 265, 376, 438, 467, 689, 860, 1327, 1410
NgoMIV GCCGGC 2 cut(s) 153, 1560
NlaIV GGNNCC 1 cut(s) 371
NmuCI GTSAC 1 cut(s) 1240
NruI TCGCGA 1 cut(s) 1158
NsiI ATGCAT 2 cut(s) 1474, 1507
NspI RCATGY 6 cut(s) 590, 1026, 1115, 1358, 1389, 1509
PaeI GCATGC 3 cut(s) 590, 1115, 1358
PaeR7I CTCGAG 1 cut(s) 238
PciI ACATGT 1 cut(s) 1385
PctI GAATGC 1 cut(s) 799
PdiI GCCGGC 2 cut(s) 155, 1562
PfeI GAWTC 5 cut(s) 53, 522, 800, 842, 1549
PkrI GCNGC 4 cut(s) 101, 436, 599, 894
PleI GAGTC 4 cut(s) 15, 230, 271, 303
PpsI GAGTC 4 cut(s) 15, 230, 271, 303
PscI ACATGT 1 cut(s) 1385
Psp1406I AACGTT 1 cut(s) 703
PspN4I GGNNCC 1 cut(s) 371
PspPI GGNCC 2 cut(s) 216, 539
PstI CTGCAG 1 cut(s) 835
PstNI CAGNNNCTG 1 cut(s) 1448
PsuI RGATCY 2 cut(s) 265, 467
RruI TCGCGA 1 cut(s) 1158
RsaI GTAC 6 cut(s) 457, 714, 858, 1054, 1062, 1427
RsaNI GTAC 6 cut(s) 456, 713, 857, 1053, 1061, 1426
RseI CAYNNNNRTG 2 cut(s) 1384, 1524
SaqAI TTAA 3 cut(s) 512, 608, 683
SatI GCNGC 4 cut(s) 100, 435, 598, 893
Sau3AI GATC 9 cut(s) 258, 265, 376, 438, 467, 689, 860, 1327, 1410
Sau96I GGNCC 2 cut(s) 216, 539
ScaI AGTACT 1 cut(s) 457
SchI GAGTC 4 cut(s) 16, 230, 271, 304
SfaNI GCATC 8 cut(s) 549, 568, 613, 1026, 1332, 1459, 1460, 1481
SfcI CTRYAG 2 cut(s) 517, 831
Sfr274I CTCGAG 1 cut(s) 238
SinI GGWCC 1 cut(s) 539
SlaI CTCGAG 1 cut(s) 238
SmiMI CAYNNNNRTG 2 cut(s) 1384, 1524
SmlI CTYRAG 3 cut(s) 238, 279, 1116
SmoI CTYRAG 3 cut(s) 238, 279, 1116
SphI GCATGC 3 cut(s) 590, 1115, 1358
Sse9I AATT 9 cut(s) 116, 139, 360, 480, 743, 971, 1197, 1323, 1486
SsiI CCGC 3 cut(s) 797, 963, 1098
SspMI CTAG 3 cut(s) 62, 1194, 1569
TaaI ACNGT 3 cut(s) 460, 856, 1073
TaiI ACGT 1 cut(s) 706
TaqI TCGA 5 cut(s) 68, 239, 730, 1330, 1510
TasI AATT 9 cut(s) 116, 139, 360, 480, 743, 971, 1197, 1323, 1486
TatI WGTACW 4 cut(s) 455, 712, 1052, 1425
TfiI GAWTC 5 cut(s) 53, 522, 800, 842, 1549
Tru1I TTAA 3 cut(s) 512, 608, 683
Tru9I TTAA 3 cut(s) 512, 608, 683
TscAI CASTG 5 cut(s) 813, 835, 924, 1076, 1353
TseFI GTSAC 1 cut(s) 1240
TseI GCWGC 4 cut(s) 99, 434, 597, 892
Tsp45I GTSAC 1 cut(s) 1240
TspDTI ATGAA 7 cut(s) 107, 133, 798, 942, 1230, 1245, 1542
TspRI CASTG 5 cut(s) 813, 835, 924, 1076, 1353
VpaK11BI GGWCC 1 cut(s) 539
XapI RAATTY 1 cut(s) 1197
XbaI TCTAGA 1 cut(s) 1193
XceI RCATGY 6 cut(s) 590, 1026, 1115, 1358, 1389, 1509
XhoI CTCGAG 1 cut(s) 238
XspI CTAG 3 cut(s) 62, 1194, 1569
ZrmI AGTACT 1 cut(s) 457
Zsp2I ATGCAT 2 cut(s) 1474, 1507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.