Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0003425

Physical Location & Seq

Genomic Coordinates Reverse (-)

6543605 .. 6547866

Gene Structure 4262 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1077500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1311 bp

ATGTCGGATGCACATTATACCATTAGTCTGTACTACATCTTTACTTGCTTACACATACTGGAGCCTTCAGTGCTTGTCATATTGGAGTGTTTAAGTGGTACTAGAGCATCTTGCATACCCTGTGAAGAATTCTGGGCCAAGCTTGTTCCATCAGACTCGAGATATCCAGATGTTGAGATTAGTTCAGATGAGCTTGCAGTTTGCTCAAAGATCGTGTTCTCTTCTATCCGCAAATGCAAGTGGTGCAACATAACAAGGAATTCAGACCACTCTTCTGCCACAATACAAAATAAAAGTTCAAATGCAATAGTTGTTGACGGGACTGTAATCCAAGCTGAAGACACTATTATTATCAGATGCGGAAGTGAAATTACTTCAGGTCCCGATATGGAAGGATATTTGAGCTACAGGTTCAATGTATTGCCTGGTCCTGAAACTTGTCAGAAGCGGTTAAAGATTTCTATGGATGTCGAGCATGCAAAATGCTGCGTTTACTTGAATATATGGCATGATATACAGTTTGTTGGAAGAAATCACTTCCTGCTTAACATTGCGGGGGAAATACTAAAAGCAGATTCTTCACTAAAACGCTCCAACAATGAAGTGGCAACTTTAGACTCTTACGCAACAATACAATCCAATCTTGTTGTAAACTCTGGAAAACATCTTCGTGGAAAAAGGGCTCGCTCCCAGTTGGATGAAGAAGCTGATAATGCGTCAAATCCATGCCCTCAATGTGGTACTGAGTATGATGGGTTTCGCTGCAATCAAATCACTATCCATCTGCAATGTCAAGCATGCAGAGGAATGATGCCTTCGCGCACCAATAACAGTGTACCTCAACACTGTTTAGGATGTGATAAGGCATTTTGTGATTCCCCCCAAGAGGCATCAAACAGGGAGGAATATAAGGAGGCAGTGTTGGAAGCTAAAGAACACATTCTAGCTGGGGATATATTTCAGATTGTATTAAGCCAGCGTTTTGAGAGGCGAACATTTGCAGACCCATTTGAAGTTTACCGAGCATTGAGGATAGTTAATCCAAGTCCATATATGACTTACTTACAGGCTAGAGGCTGTATTCTGGTTGCTTCAAGTCCAGAAATTCTGACACGTGTCAAGAAGCAAACGATCACAAACAGGCCTCTTGCTGGGACTGTTAGAAGAGGAAAAACACCTAAAGAGGATTTATTGCTGGAAAAGCAGCTTTTGAATGATGAAAAGCAATTTGCAGAGCATATTATGCTTGTAGACTTGGGGAGGAATGATGTCGGGAAGGTTTATTATCTTTCCCTTCTCGTATTTGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000075 GO:0000166 GO:0000209 GO:0000278 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016604 GO:0016605 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0022402 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031647 GO:0031648 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0036094 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045732 GO:0045786 GO:0045862 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051726 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0097159 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1903047 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:2000058 GO:2000060

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4 zf-RING_2

Protein Analysis

437

Amino Acids

49.08

Weight (kDa)

6.43

Isoelectric Point (pI)

53.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-CRD	PF17979	243 - 327	1.7e-18	Cysteine rich domain with multizinc binding regions
Chorismate_bind	PF00425	300 - 427	1.7e-35	chorismate binding enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003425)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47570 AT1G47570 AT1G47570
fragaria_vesca	FvH4_3g38370 FvH4_3g38370 FvH4_3g38370
malus_domestica	MD00G1120300.v1.1 MD00G1120800.v1.1 MD03G1072300.v1.1 MD11G1077300.v1.1 MD11G1077500.v1.1 MD11G1077800.v1.1 MD11G1078400.v1.1 MD11G1078600.v1.1
prunus_persica	Prupe.6G059200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0157061 RchiOBHm_Chr2g0157071 RchiOBHm_Chr5g0068971
rosa_laevigata	RLG00000036026
rosa_multiflora	Rmu_sc0000853.1_g000001 Rmu_sc0006282.1_g000033
rosa_roxburghii	Rroxscaffold_1G00011750
rosa_rugosa	Rorug05G0395200 Rorug05G0395300.1 Rorug05G0395400 Rorug07G0214500.1 Rorug07G0214600
rosa_samantha	Rh2BG537300 Rh2DG546100 Rh5BG471000 Rh5CG493600 Rh5DG483700 Rh7BG348000
rosa_wichuraiana	Rw0G006150 Rw2G043230 Rw5G042220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1251
AccII	CGCG	1 cut(s)	820
AciI	CCGC	4 cut(s)	229, 360, 448, 554
AcsI	RAATTY	3 cut(s)	128, 259, 1104
AcuI	CTGAAG	3 cut(s)	51, 357, 360
AcvI	CACGTG	1 cut(s)	1115
AfaI	GTAC	4 cut(s)	32, 100, 742, 837
AfiI	CCNNNNNNNGG	3 cut(s)	737, 886, 1151
AflIII	ACRYGT	2 cut(s)	1112, 1114
AgsI	TTSAA	6 cut(s)	300, 415, 499, 1013, 1095, 1213
AjnI	CCWGG	1 cut(s)	424
AluBI	AGCT	8 cut(s)	142, 193, 335, 405, 707, 929, 947, 1207
AluI	AGCT	8 cut(s)	142, 193, 335, 405, 707, 929, 947, 1207
Ama87I	CYCGRG	1 cut(s)	157
AoxI	GGCC	2 cut(s)	135, 1142
ApeKI	GCWGC	3 cut(s)	486, 762, 1204
ApoI	RAATTY	3 cut(s)	128, 259, 1104
Asp700I	GAANNNNTTC	1 cut(s)	939
AspLEI	GCGC	1 cut(s)	822
AspS9I	GGNCC	3 cut(s)	135, 380, 428
AvaI	CYCGRG	1 cut(s)	157
AvaII	GGWCC	2 cut(s)	380, 428
BanII	GRGCYC	1 cut(s)	685
BarI	GAAGNNNNNNTAC	2 cut(s)	355, 387
BbrPI	CACGTG	1 cut(s)	1115
BbsI	GAAGAC	1 cut(s)	345
BbvI	GCAGC	3 cut(s)	473, 749, 1216
BccI	CCATC	3 cut(s)	157, 746, 789
BciT130I	CCWGG	1 cut(s)	426
BfaI	CTAG	3 cut(s)	102, 944, 1071
BfmI	CTRYAG	1 cut(s)	406
BisI	GCNGC	3 cut(s)	487, 763, 1205
BlsI	GCNGC	3 cut(s)	488, 764, 1206
Bme1390I	CCNGG	1 cut(s)	426
Bme18I	GGWCC	2 cut(s)	380, 428
BmeT110I	CYCGRG	1 cut(s)	157
BmgT120I	GGNCC	3 cut(s)	135, 380, 428
BmiI	GGNNCC	2 cut(s)	63, 382
BmrFI	CCNGG	1 cut(s)	426
BmrI	ACTGGG	1 cut(s)	685
BmsI	GCATC	4 cut(s)	116, 347, 801, 899
BmuI	ACTGGG	1 cut(s)	685
BoxI	GACNNNNGTC	1 cut(s)	1115
BpiI	GAAGAC	1 cut(s)	345
BpmI	CTGGAG	1 cut(s)	80
BsaAI	YACGTR	1 cut(s)	1115
BsaXI	ACNNNNNCTCC	2 cut(s)	905, 935
Bsc4I	CCNNNNNNNGG	3 cut(s)	737, 886, 1151
Bse1I	ACTGG	2 cut(s)	63, 691
Bse3DI	GCAATG	2 cut(s)	549, 794
BseBI	CCWGG	1 cut(s)	426
BseGI	GGATG	4 cut(s)	13, 472, 703, 860
BseLI	CCNNNNNNNGG	3 cut(s)	737, 886, 1151
BseMI	GCAATG	2 cut(s)	549, 794
BseMII	CTCAG	1 cut(s)	735
BseNI	ACTGG	2 cut(s)	63, 691
BseXI	GCAGC	3 cut(s)	473, 749, 1216
BseYI	CCCAGC	2 cut(s)	947, 1151
Bsh1236I	CGCG	1 cut(s)	820
BshFI	GGCC	2 cut(s)	137, 1144
BsiHKCI	CYCGRG	1 cut(s)	157
BslFI	GGGAC	3 cut(s)	334, 366, 1168
BslI	CCNNNNNNNGG	3 cut(s)	737, 886, 1151
BsmFI	GGGAC	3 cut(s)	334, 366, 1168
BsnI	GGCC	2 cut(s)	137, 1144
BsoBI	CYCGRG	1 cut(s)	157
Bsp1286I	GDGCHC	1 cut(s)	685
Bsp143I	GATC	2 cut(s)	210, 1131
BspACI	CCGC	4 cut(s)	229, 360, 448, 554
BspANI	GGCC	2 cut(s)	137, 1144
BspCNI	CTCAG	1 cut(s)	736
BspFNI	CGCG	1 cut(s)	820
BspLI	GGNNCC	2 cut(s)	63, 382
BsrDI	GCAATG	2 cut(s)	549, 794
BsrI	ACTGG	2 cut(s)	63, 691
BssMI	GATC	2 cut(s)	210, 1131
Bst2UI	CCWGG	1 cut(s)	426
Bst4CI	ACNGT	5 cut(s)	325, 519, 833, 848, 1159
Bst6I	CTCTTC	3 cut(s)	226, 277, 1159
BstAPI	GCANNNNNTGC	2 cut(s)	243, 1243
BstBAI	YACGTR	1 cut(s)	1115
BstC8I	GCNNGC	5 cut(s)	195, 477, 685, 799, 977
BstDEI	CTNAG	1 cut(s)	744
BstF5I	GGATG	4 cut(s)	13, 472, 703, 860
BstFNI	CGCG	1 cut(s)	820
BstHHI	GCGC	1 cut(s)	822
BstKTI	GATC	2 cut(s)	213, 1134
BstMBI	GATC	2 cut(s)	210, 1131
BstMWI	GCNNNNNNNGC	5 cut(s)	70, 243, 713, 1201, 1243
BstNI	CCWGG	1 cut(s)	426
BstNSI	RCATGY	2 cut(s)	479, 801
BstPAI	GACNNNNGTC	1 cut(s)	1115
BstSCI	CCNGG	1 cut(s)	424
BstSFI	CTRYAG	1 cut(s)	406
BstUI	CGCG	1 cut(s)	820
BstV1I	GCAGC	3 cut(s)	473, 749, 1216
BstV2I	GAAGAC	1 cut(s)	345
BsuRI	GGCC	2 cut(s)	137, 1144
BtsCI	GGATG	4 cut(s)	13, 472, 703, 860
BtsI	GCAGTG	1 cut(s)	924
BtsIMutI	CAGTG	4 cut(s)	75, 838, 844, 924
Cac8I	GCNNGC	5 cut(s)	195, 477, 685, 799, 977
CfoI	GCGC	1 cut(s)	822
Cfr13I	GGNCC	3 cut(s)	135, 380, 428
CseI	GACGC	1 cut(s)	705
Csp6I	GTAC	4 cut(s)	31, 99, 741, 836
CviAII	CATG	4 cut(s)	476, 509, 726, 798
CviQI	GTAC	4 cut(s)	31, 99, 741, 836
DdeI	CTNAG	1 cut(s)	744
DpnI	GATC	2 cut(s)	212, 1133
DpnII	GATC	2 cut(s)	210, 1131
Eam1104I	CTCTTC	3 cut(s)	226, 277, 1159
EarI	CTCTTC	3 cut(s)	226, 277, 1159
Eco147I	AGGCCT	1 cut(s)	1144
Eco24I	GRGCYC	1 cut(s)	685
Eco32I	GATATC	1 cut(s)	164
Eco47I	GGWCC	2 cut(s)	380, 428
Eco57I	CTGAAG	3 cut(s)	51, 357, 360
Eco72I	CACGTG	1 cut(s)	1115
Eco88I	CYCGRG	1 cut(s)	157
EcoO109I	RGGNCCY	1 cut(s)	380
EcoRI	GAATTC	2 cut(s)	128, 259
EcoRII	CCWGG	1 cut(s)	424
EcoRV	GATATC	1 cut(s)	164
EcoT38I	GRGCYC	1 cut(s)	685
FaeI	CATG	4 cut(s)	479, 512, 729, 801
FaqI	GGGAC	3 cut(s)	334, 366, 1168
FatI	CATG	4 cut(s)	475, 508, 725, 797
FauI	CCCGC	1 cut(s)	547
FblI	GTMKAC	1 cut(s)	1251
Fnu4HI	GCNGC	3 cut(s)	487, 763, 1205
FokI	GGATG	4 cut(s)	20, 479, 710, 867
FriOI	GRGCYC	1 cut(s)	685
Fsp4HI	GCNGC	3 cut(s)	487, 763, 1205
FspBI	CTAG	3 cut(s)	102, 944, 1071
GlaI	GCGC	1 cut(s)	821
GluI	GCNGC	3 cut(s)	487, 763, 1205
GsaI	CCCAGC	2 cut(s)	951, 1155
GsuI	CTGGAG	1 cut(s)	80
HaeIII	GGCC	2 cut(s)	137, 1144
HgaI	GACGC	1 cut(s)	705
HhaI	GCGC	1 cut(s)	822
Hin1II	CATG	4 cut(s)	479, 512, 729, 801
Hin6I	GCGC	1 cut(s)	820
HinP1I	GCGC	1 cut(s)	820
HincII	GTYRAC	1 cut(s)	316
HindII	GTYRAC	1 cut(s)	316
HindIII	AAGCTT	1 cut(s)	140
HinfI	GANTC	4 cut(s)	155, 575, 617, 875
Hpy166II	GTNNAC	6 cut(s)	316, 493, 652, 836, 1018, 1252
Hpy188I	TCNGA	8 cut(s)	7, 154, 187, 265, 356, 444, 963, 1110
Hpy188III	TCNNGA	8 cut(s)	159, 167, 383, 431, 657, 1100, 1120, 1273
Hpy8I	GTNNAC	6 cut(s)	316, 493, 652, 836, 1018, 1252
HpyAV	CCTTC	5 cut(s)	75, 386, 825, 1270, 1304
HpyCH4III	ACNGT	5 cut(s)	325, 519, 833, 848, 1159
HpyCH4IV	ACGT	1 cut(s)	1114
HpyF10VI	GCNNNNNNNGC	5 cut(s)	70, 243, 713, 1201, 1243
HpyF3I	CTNAG	1 cut(s)	744
HpySE526I	ACGT	1 cut(s)	1114
Hsp92II	CATG	4 cut(s)	479, 512, 729, 801
HspAI	GCGC	1 cut(s)	820
Kzo9I	GATC	2 cut(s)	210, 1131
LmnI	GCTCC	3 cut(s)	61, 596, 692
Lsp1109I	GCAGC	3 cut(s)	473, 749, 1216
LweI	GCATC	4 cut(s)	116, 347, 801, 899
MaeI	CTAG	3 cut(s)	102, 944, 1071
MaeII	ACGT	1 cut(s)	1114
MalI	GATC	2 cut(s)	212, 1133
MboI	GATC	2 cut(s)	210, 1131
MboII	GAAGA	9 cut(s)	137, 213, 264, 350, 540, 570, 659, 713, 1176
MhlI	GDGCHC	1 cut(s)	685
MluCI	AATT	5 cut(s)	128, 259, 369, 1104, 1226
MlyI	GAGTC	2 cut(s)	149, 611
MmeI	TCCRAC	4 cut(s)	505, 618, 675, 903
MroXI	GAANNNNTTC	1 cut(s)	939
MseI	TTAA	5 cut(s)	92, 452, 546, 971, 1038
MslI	CAYNNNNRTG	1 cut(s)	669
MspR9I	CCNGG	1 cut(s)	426
MvaI	CCWGG	1 cut(s)	426
MvnI	CGCG	1 cut(s)	820
MwoI	GCNNNNNNNGC	5 cut(s)	70, 243, 713, 1201, 1243
NdeII	GATC	2 cut(s)	210, 1131
NlaIII	CATG	4 cut(s)	479, 512, 729, 801
NlaIV	GGNNCC	2 cut(s)	63, 382
NspI	RCATGY	2 cut(s)	479, 801
PaeI	GCATGC	2 cut(s)	479, 801
PaeR7I	CTCGAG	1 cut(s)	157
PceI	AGGCCT	1 cut(s)	1144
PdmI	GAANNNNTTC	1 cut(s)	939
PfeI	GAWTC	2 cut(s)	575, 875
PkrI	GCNGC	3 cut(s)	488, 764, 1206
PleI	GAGTC	2 cut(s)	149, 611
PmaCI	CACGTG	1 cut(s)	1115
PmlI	CACGTG	1 cut(s)	1115
PpsI	GAGTC	2 cut(s)	149, 611
Ppu21I	YACGTR	1 cut(s)	1115
PpuMI	RGGWCCY	1 cut(s)	380
PshAI	GACNNNNGTC	1 cut(s)	1115
Psp5II	RGGWCCY	1 cut(s)	380
Psp6I	CCWGG	1 cut(s)	424
PspCI	CACGTG	1 cut(s)	1115
PspFI	CCCAGC	2 cut(s)	947, 1151
PspGI	CCWGG	1 cut(s)	424
PspN4I	GGNNCC	2 cut(s)	63, 382
PspPI	GGNCC	3 cut(s)	135, 380, 428
PspPPI	RGGWCCY	1 cut(s)	380
RsaI	GTAC	4 cut(s)	32, 100, 742, 837
RsaNI	GTAC	4 cut(s)	31, 99, 741, 836
RseI	CAYNNNNRTG	1 cut(s)	669
SaqAI	TTAA	5 cut(s)	92, 452, 546, 971, 1038
SatI	GCNGC	3 cut(s)	487, 763, 1205
Sau3AI	GATC	2 cut(s)	210, 1131
Sau96I	GGNCC	3 cut(s)	135, 380, 428
SchI	GAGTC	2 cut(s)	149, 611
ScrFI	CCNGG	1 cut(s)	426
SduI	GDGCHC	1 cut(s)	685
SfaNI	GCATC	4 cut(s)	116, 347, 801, 899
SfcI	CTRYAG	1 cut(s)	406
Sfr274I	CTCGAG	1 cut(s)	157
SinI	GGWCC	2 cut(s)	380, 428
SlaI	CTCGAG	1 cut(s)	157
SmiMI	CAYNNNNRTG	1 cut(s)	669
SmlI	CTYRAG	1 cut(s)	157
SmoI	CTYRAG	1 cut(s)	157
SphI	GCATGC	2 cut(s)	479, 801
Sse9I	AATT	5 cut(s)	128, 259, 369, 1104, 1226
SseBI	AGGCCT	1 cut(s)	1144
SsiI	CCGC	4 cut(s)	229, 360, 448, 554
SspMI	CTAG	3 cut(s)	102, 944, 1071
StuI	AGGCCT	1 cut(s)	1144
StyD4I	CCNGG	1 cut(s)	424
TaaI	ACNGT	5 cut(s)	325, 519, 833, 848, 1159
TaiI	ACGT	1 cut(s)	1117
TaqI	TCGA	2 cut(s)	158, 471
TasI	AATT	5 cut(s)	128, 259, 369, 1104, 1226
TatI	WGTACW	1 cut(s)	30
TfiI	GAWTC	2 cut(s)	575, 875
Tru1I	TTAA	5 cut(s)	92, 452, 546, 971, 1038
Tru9I	TTAA	5 cut(s)	92, 452, 546, 971, 1038
TscAI	CASTG	4 cut(s)	75, 838, 851, 924
TseI	GCWGC	3 cut(s)	486, 762, 1204
TspDTI	ATGAA	3 cut(s)	615, 714, 1233
TspRI	CASTG	4 cut(s)	75, 838, 851, 924
VpaK11BI	GGWCC	2 cut(s)	380, 428
XapI	RAATTY	3 cut(s)	128, 259, 1104
XceI	RCATGY	2 cut(s)	479, 801
XcmI	CCANNNNNNNNNTGG	1 cut(s)	601
XhoI	CTCGAG	1 cut(s)	157
XmiI	GTMKAC	1 cut(s)	1251
XmnI	GAANNNNTTC	1 cut(s)	939
XspI	CTAG	3 cut(s)	102, 944, 1071

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.