MD00G1120300.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0003425
Physical Location & Seq
Reverse (-)
25862577 .. 25863411
835 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1120300.v1.1.491

Sequence Viewer

Length: 717 bp
ATGAAATCTTCACTAAAAAACACCAACGATGAAGTGGCAATTTTAGACTCTAGTGCAACAATACAATCTAATCTTGTCATAAACTCTGAAAAACATCTTCGTCGAAAAAGGGCTCGCTCCCAGGTGGATGAAGAAGCTGATAATCCATCAAATCCATGCCCTCAATGTGGTACTGAATATGGTGGGTTCCGCTGCAATCAAACCACTATCCATCTGCAATGTCAAGCATGCGGAGGAATGATGCCTTTGCGCACCAATAACAATAGTGATTCTCACCGTGTGTGCAGTCGCGAGACTTTTAAACCAATCTCAGAATGGGCCATCTCTGGAATCCCTACCTCAGCGCATGAAAACAATCAACATGAACAAGTTATCACTGAAAAGTGTATTGCACAGTTGGGGAGAACATTGCAGGATGTCGTTGCAGAATGGCTTGCAAAGTTGAACAACAGAGAAATTGATCGAACAAGGATGCCCCTGAATCATGCTGAGGCGATAACTTCTGGGACATCCACGTGCAATTACTGTTATGAGAAGCTGATCTCCCTACTCTTGTACTGGTTTCGGATTTTGATTCCCAAATACCATCTACCCACAGAGGCATCAAACAGGGAAAATTGCTGGTATGGATATGCATGTCGGACACAGCACCATAATGAAGAACATGCTTGTAAAATAAATCATATGTGCCGGCCAACTAGGGGTACTAATATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000166 GO:0000209 GO:0000278 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016604 GO:0016605 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0022402 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031647 GO:0031648 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0036094 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045732 GO:0045786 GO:0045862 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051726 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0097159 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1903047 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-RING_2

Protein Analysis

239

Amino Acids

27.17

Weight (kDa)

7.95

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CRD PF17979 53 - 192 9.7e-33 Cysteine rich domain with multizinc binding regions
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 251
AccII CGCG 1 cut(s) 291
AciI CCGC 2 cut(s) 190, 231
AcoI YGGCCR 1 cut(s) 692
AcvI CACGTG 1 cut(s) 516
AdeI CACNNNGTG 1 cut(s) 280
AfaI GTAC 3 cut(s) 172, 557, 706
AfiI CCNNNNNNNGG 2 cut(s) 167, 701
AgsI TTSAA 1 cut(s) 445
AjnI CCWGG 1 cut(s) 120
AluBI AGCT 2 cut(s) 137, 538
AluI AGCT 2 cut(s) 137, 538
Alw26I GTCTC 1 cut(s) 287
AoxI GGCC 2 cut(s) 318, 692
ApeKI GCWGC 1 cut(s) 192
AspLEI GCGC 2 cut(s) 252, 346
AspS9I GGNCC 1 cut(s) 318
AsuHPI GGTGA 1 cut(s) 266
BanII GRGCYC 1 cut(s) 115
BbrPI CACGTG 1 cut(s) 516
BbvCI CCTCAGC 2 cut(s) 340, 489
BbvI GCAGC 1 cut(s) 179
BccI CCATC 4 cut(s) 154, 219, 329, 594
BciT130I CCWGG 1 cut(s) 122
BcoDI GTCTC 1 cut(s) 287
BfaI CTAG 2 cut(s) 51, 699
BisI GCNGC 1 cut(s) 193
BlsI GCNGC 1 cut(s) 194
Bme1390I CCNGG 1 cut(s) 122
BmgT120I GGNCC 1 cut(s) 318
BmiI GGNNCC 1 cut(s) 188
BmrFI CCNGG 1 cut(s) 122
BmsI GCATC 3 cut(s) 231, 462, 611
Bpu10I CCTNAGC 2 cut(s) 340, 489
BsaAI YACGTR 1 cut(s) 516
BsaJI CCNNGG 1 cut(s) 120
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 701
Bse118I RCCGGY 1 cut(s) 690
Bse1I ACTGG 1 cut(s) 563
Bse3DI GCAATG 2 cut(s) 224, 407
BseBI CCWGG 1 cut(s) 122
BseDI CCNNGG 1 cut(s) 120
BseGI GGATG 4 cut(s) 133, 421, 477, 509
BseLI CCNNNNNNNGG 2 cut(s) 167, 701
BseMI GCAATG 2 cut(s) 224, 407
BseMII CTCAG 3 cut(s) 324, 354, 480
BseNI ACTGG 1 cut(s) 563
BseXI GCAGC 1 cut(s) 179
BsgI GTGCAG 1 cut(s) 304
Bsh1236I CGCG 1 cut(s) 291
BshFI GGCC 2 cut(s) 320, 694
BsiSI CCGG 1 cut(s) 691
BslFI GGGAC 1 cut(s) 520
BslI CCNNNNNNNGG 2 cut(s) 167, 701
BsmAI GTCTC 1 cut(s) 287
BsmFI GGGAC 1 cut(s) 520
BsnI GGCC 2 cut(s) 320, 694
Bsp1286I GDGCHC 1 cut(s) 115
Bsp143I GATC 2 cut(s) 460, 540
Bsp68I TCGCGA 1 cut(s) 291
BspACI CCGC 2 cut(s) 190, 231
BspANI GGCC 2 cut(s) 320, 694
BspCNI CTCAG 3 cut(s) 323, 353, 481
BspFNI CGCG 1 cut(s) 291
BspLI GGNNCC 1 cut(s) 188
BsrDI GCAATG 2 cut(s) 224, 407
BsrFI RCCGGY 1 cut(s) 690
BsrI ACTGG 1 cut(s) 563
BssAI RCCGGY 1 cut(s) 690
BssECI CCNNGG 1 cut(s) 120
BssMI GATC 2 cut(s) 460, 540
Bst2UI CCWGG 1 cut(s) 122
Bst4CI ACNGT 3 cut(s) 278, 396, 527
BstBAI YACGTR 1 cut(s) 516
BstC8I GCNNGC 4 cut(s) 115, 229, 435, 692
BstDEI CTNAG 3 cut(s) 310, 340, 489
BstF5I GGATG 4 cut(s) 133, 421, 477, 509
BstFNI CGCG 1 cut(s) 291
BstHHI GCGC 2 cut(s) 252, 346
BstKTI GATC 2 cut(s) 463, 543
BstMAI GTCTC 1 cut(s) 287
BstMBI GATC 2 cut(s) 460, 540
BstNI CCWGG 1 cut(s) 122
BstNSI RCATGY 3 cut(s) 231, 639, 668
BstSCI CCNGG 1 cut(s) 120
BstUI CGCG 1 cut(s) 291
BstV1I GCAGC 1 cut(s) 179
BsuRI GGCC 2 cut(s) 320, 694
BtsCI GGATG 4 cut(s) 133, 421, 477, 509
BtsIMutI CAGTG 1 cut(s) 375
BtuMI TCGCGA 1 cut(s) 291
Cac8I GCNNGC 4 cut(s) 115, 229, 435, 692
CfoI GCGC 2 cut(s) 252, 346
Cfr10I RCCGGY 1 cut(s) 690
Cfr13I GGNCC 1 cut(s) 318
Csp6I GTAC 3 cut(s) 171, 556, 705
CviAII CATG 7 cut(s) 156, 228, 347, 362, 485, 636, 665
CviJI RGCY 6 cut(s) 113, 137, 320, 433, 538, 694
CviKI_1 RGCY 6 cut(s) 113, 137, 320, 433, 538, 694
CviQI GTAC 3 cut(s) 171, 556, 705
DdeI CTNAG 3 cut(s) 310, 340, 489
DpnI GATC 2 cut(s) 462, 542
DpnII GATC 2 cut(s) 460, 540
DraI TTTAAA 1 cut(s) 301
DraIII CACNNNGTG 1 cut(s) 280
EaeI YGGCCR 1 cut(s) 692
Eco24I GRGCYC 1 cut(s) 115
Eco72I CACGTG 1 cut(s) 516
EcoRII CCWGG 1 cut(s) 120
EcoT22I ATGCAT 1 cut(s) 637
EcoT38I GRGCYC 1 cut(s) 115
FaeI CATG 7 cut(s) 159, 231, 350, 365, 488, 639, 668
FaqI GGGAC 1 cut(s) 520
FatI CATG 7 cut(s) 155, 227, 346, 361, 484, 635, 664
FauNDI CATATG 1 cut(s) 684
Fnu4HI GCNGC 1 cut(s) 193
FokI GGATG 4 cut(s) 140, 428, 484, 496
FriOI GRGCYC 1 cut(s) 115
Fsp4HI GCNGC 1 cut(s) 193
FspBI CTAG 2 cut(s) 51, 699
FspI TGCGCA 1 cut(s) 251
GlaI GCGC 2 cut(s) 251, 345
GluI GCNGC 1 cut(s) 193
HaeIII GGCC 2 cut(s) 320, 694
HapII CCGG 1 cut(s) 691
HhaI GCGC 2 cut(s) 252, 346
Hin1II CATG 7 cut(s) 159, 231, 350, 365, 488, 639, 668
Hin6I GCGC 2 cut(s) 250, 344
HinP1I GCGC 2 cut(s) 250, 344
HinfI GANTC 5 cut(s) 47, 269, 330, 481, 574
HpaII CCGG 1 cut(s) 691
HphI GGTGA 1 cut(s) 266
Hpy188I TCNGA 4 cut(s) 88, 313, 567, 642
Hpy188III TCNNGA 2 cut(s) 290, 327
Hpy99I CGWCG 1 cut(s) 105
HpyCH4III ACNGT 3 cut(s) 278, 396, 527
HpyCH4IV ACGT 1 cut(s) 515
HpyF3I CTNAG 3 cut(s) 310, 340, 489
HpySE526I ACGT 1 cut(s) 515
Hsp92II CATG 7 cut(s) 159, 231, 350, 365, 488, 639, 668
HspAI GCGC 2 cut(s) 250, 344
KroI GCCGGC 1 cut(s) 690
KroNI GCCGGC 1 cut(s) 692
Kzo9I GATC 2 cut(s) 460, 540
LmnI GCTCC 1 cut(s) 122
Lsp1109I GCAGC 1 cut(s) 179
LweI GCATC 3 cut(s) 231, 462, 611
MaeI CTAG 2 cut(s) 51, 699
MaeII ACGT 1 cut(s) 515
MalI GATC 2 cut(s) 462, 542
MboI GATC 2 cut(s) 460, 540
MboII GAAGA 3 cut(s) 89, 143, 671
MhlI GDGCHC 1 cut(s) 115
MluCI AATT 4 cut(s) 39, 456, 520, 616
MlyI GAGTC 1 cut(s) 41
MmeI TCCRAC 1 cut(s) 620
MnlI CCTC 5 cut(s) 171, 227, 349, 484, 592
Mph1103I ATGCAT 1 cut(s) 637
MroNI GCCGGC 1 cut(s) 690
MseI TTAA 1 cut(s) 300
MslI CAYNNNNRTG 2 cut(s) 514, 654
MspA1I CMGCKG 1 cut(s) 192
MspI CCGG 1 cut(s) 691
MspR9I CCNGG 1 cut(s) 122
MvaI CCWGG 1 cut(s) 122
MvnI CGCG 1 cut(s) 291
NaeI GCCGGC 1 cut(s) 692
NdeI CATATG 1 cut(s) 684
NdeII GATC 2 cut(s) 460, 540
NgoMIV GCCGGC 1 cut(s) 690
NlaIII CATG 7 cut(s) 159, 231, 350, 365, 488, 639, 668
NlaIV GGNNCC 1 cut(s) 188
NruI TCGCGA 1 cut(s) 291
NsbI TGCGCA 1 cut(s) 251
NsiI ATGCAT 1 cut(s) 637
NspI RCATGY 3 cut(s) 231, 639, 668
PaeI GCATGC 1 cut(s) 231
PdiI GCCGGC 1 cut(s) 692
PfeI GAWTC 4 cut(s) 269, 330, 481, 574
PkrI GCNGC 1 cut(s) 194
PleI GAGTC 1 cut(s) 41
PmaCI CACGTG 1 cut(s) 516
PmlI CACGTG 1 cut(s) 516
PpsI GAGTC 1 cut(s) 41
Ppu21I YACGTR 1 cut(s) 516
Psp6I CCWGG 1 cut(s) 120
PspCI CACGTG 1 cut(s) 516
PspGI CCWGG 1 cut(s) 120
PspN4I GGNNCC 1 cut(s) 188
PspPI GGNCC 1 cut(s) 318
RruI TCGCGA 1 cut(s) 291
RsaI GTAC 3 cut(s) 172, 557, 706
RsaNI GTAC 3 cut(s) 171, 556, 705
RseI CAYNNNNRTG 2 cut(s) 514, 654
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 1 cut(s) 193
Sau3AI GATC 2 cut(s) 460, 540
Sau96I GGNCC 1 cut(s) 318
SchI GAGTC 1 cut(s) 41
ScrFI CCNGG 1 cut(s) 122
SduI GDGCHC 1 cut(s) 115
SetI ASST 5 cut(s) 126, 139, 341, 518, 540
SfaNI GCATC 3 cut(s) 231, 462, 611
SmiMI CAYNNNNRTG 2 cut(s) 514, 654
SphI GCATGC 1 cut(s) 231
Sse9I AATT 4 cut(s) 39, 456, 520, 616
SsiI CCGC 2 cut(s) 190, 231
SspMI CTAG 2 cut(s) 51, 699
StyD4I CCNGG 1 cut(s) 120
TaaI ACNGT 3 cut(s) 278, 396, 527
TaiI ACGT 1 cut(s) 518
TaqI TCGA 2 cut(s) 103, 463
TasI AATT 4 cut(s) 39, 456, 520, 616
TatI WGTACW 1 cut(s) 555
TfiI GAWTC 4 cut(s) 269, 330, 481, 574
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 1 cut(s) 382
TseI GCWGC 1 cut(s) 192
TspDTI ATGAA 6 cut(s) 17, 45, 144, 363, 378, 672
TspRI CASTG 1 cut(s) 382
XceI RCATGY 3 cut(s) 231, 639, 668
XcmI CCANNNNNNNNNTGG 2 cut(s) 31, 312
XspI CTAG 2 cut(s) 51, 699
Zsp2I ATGCAT 1 cut(s) 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.