MD11G1077800.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003425
Physical Location & Seq
Reverse (-)
6636443 .. 6639088
2646 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1077800.v1.1.491

Sequence Viewer

Length: 282 bp
ATGCCCCTGAATCATGCTGAAGCAATAACTTCTGGGTCACCTACGTGCAATTACTGTTATGAGAAGCTGATCTCCTTCCTCTTGTACTGGTTTCGGATTTCGATTCCCAAATATCATCTACCCACAGAGGCATCAGACAGGGAAAATTGCTGGTATGGATATGCATGTCGGACACAGCACCATAATGAAGAACATGCTTGTAAAGAAATCATGTGTGCCGGCCAACTAGGGGTTCTTATATGTAGATCTTCTTTCTCTTGTTTGCTCTGTTTTGAGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000166 GO:0000209 GO:0000278 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016604 GO:0016605 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0022402 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031647 GO:0031648 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0036094 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045732 GO:0045786 GO:0045862 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051726 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0097159 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1903047 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-RING_2

Protein Analysis

94

Amino Acids

10.76

Weight (kDa)

6.16

Isoelectric Point (pI)

52.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CRD PF17979 3 - 35 5.6e-06 Cysteine rich domain with multizinc binding regions
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 220
AcuI CTGAAG 1 cut(s) 39
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 229
AjuI GAANNNNNNNTTGG 2 cut(s) 216, 248
AleI CACNNNNGTG 1 cut(s) 43
AluBI AGCT 1 cut(s) 67
AluI AGCT 1 cut(s) 67
AoxI GGCC 1 cut(s) 220
AsuHPI GGTGA 1 cut(s) 30
BfaI CTAG 1 cut(s) 227
BglII AGATCT 1 cut(s) 245
BmsI GCATC 1 cut(s) 140
BsaAI YACGTR 1 cut(s) 45
Bsc4I CCNNNNNNNGG 1 cut(s) 229
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 1 cut(s) 92
BseLI CCNNNNNNNGG 1 cut(s) 229
BseNI ACTGG 1 cut(s) 92
BshFI GGCC 1 cut(s) 222
BsiSI CCGG 1 cut(s) 219
BslI CCNNNNNNNGG 1 cut(s) 229
BsnI GGCC 1 cut(s) 222
Bsp143I GATC 2 cut(s) 69, 245
BspANI GGCC 1 cut(s) 222
BsrFI RCCGGY 1 cut(s) 218
BsrI ACTGG 1 cut(s) 92
BssAI RCCGGY 1 cut(s) 218
BssMI GATC 2 cut(s) 69, 245
Bst4CI ACNGT 1 cut(s) 56
BstBAI YACGTR 1 cut(s) 45
BstC8I GCNNGC 1 cut(s) 220
BstEII GGTNACC 1 cut(s) 36
BstKTI GATC 2 cut(s) 72, 248
BstMBI GATC 2 cut(s) 69, 245
BstNSI RCATGY 2 cut(s) 168, 197
BstPI GGTNACC 1 cut(s) 36
BstX2I RGATCY 1 cut(s) 245
BstYI RGATCY 1 cut(s) 245
BsuRI GGCC 1 cut(s) 222
Cac8I GCNNGC 1 cut(s) 220
Cfr10I RCCGGY 1 cut(s) 218
Csp6I GTAC 1 cut(s) 85
CviAII CATG 4 cut(s) 14, 165, 194, 211
CviJI RGCY 2 cut(s) 67, 222
CviKI_1 RGCY 2 cut(s) 67, 222
CviQI GTAC 1 cut(s) 85
DpnI GATC 2 cut(s) 71, 247
DpnII GATC 2 cut(s) 69, 245
EaeI YGGCCR 1 cut(s) 220
Eco57I CTGAAG 1 cut(s) 39
Eco91I GGTNACC 1 cut(s) 36
EcoO65I GGTNACC 1 cut(s) 36
EcoT22I ATGCAT 1 cut(s) 166
FaeI CATG 4 cut(s) 17, 168, 197, 214
FatI CATG 4 cut(s) 13, 164, 193, 210
FspBI CTAG 1 cut(s) 227
HaeIII GGCC 1 cut(s) 222
HapII CCGG 1 cut(s) 219
Hin1II CATG 4 cut(s) 17, 168, 197, 214
HinfI GANTC 2 cut(s) 10, 103
HpaII CCGG 1 cut(s) 219
HphI GGTGA 1 cut(s) 30
Hpy188I TCNGA 3 cut(s) 96, 136, 171
HpyAV CCTTC 1 cut(s) 85
HpyCH4III ACNGT 1 cut(s) 56
HpyCH4IV ACGT 1 cut(s) 44
HpyCH4V TGCA 2 cut(s) 48, 164
HpySE526I ACGT 1 cut(s) 44
Hsp92II CATG 4 cut(s) 17, 168, 197, 214
KroI GCCGGC 1 cut(s) 218
KroNI GCCGGC 1 cut(s) 220
Kzo9I GATC 2 cut(s) 69, 245
LpnPI CCDG 6 cut(s) 18, 20, 73, 124, 136, 232
LweI GCATC 1 cut(s) 140
MaeI CTAG 1 cut(s) 227
MaeII ACGT 1 cut(s) 44
MaeIII GTNAC 1 cut(s) 36
MalI GATC 2 cut(s) 71, 247
MboI GATC 2 cut(s) 69, 245
MboII GAAGA 2 cut(s) 200, 240
MflI RGATCY 1 cut(s) 245
MluCI AATT 3 cut(s) 49, 145, 277
MmeI TCCRAC 1 cut(s) 149
MnlI CCTC 2 cut(s) 89, 121
Mph1103I ATGCAT 1 cut(s) 166
MroNI GCCGGC 1 cut(s) 218
MslI CAYNNNNRTG 2 cut(s) 43, 183
MspI CCGG 1 cut(s) 219
NaeI GCCGGC 1 cut(s) 220
NdeII GATC 2 cut(s) 69, 245
NgoMIV GCCGGC 1 cut(s) 218
NlaIII CATG 4 cut(s) 17, 168, 197, 214
NmuCI GTSAC 1 cut(s) 36
NsiI ATGCAT 1 cut(s) 166
NspI RCATGY 2 cut(s) 168, 197
OliI CACNNNNGTG 1 cut(s) 43
PdiI GCCGGC 1 cut(s) 220
PfeI GAWTC 2 cut(s) 10, 103
Ppu21I YACGTR 1 cut(s) 45
PspEI GGTNACC 1 cut(s) 36
PsuI RGATCY 1 cut(s) 245
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
RseI CAYNNNNRTG 2 cut(s) 43, 183
Sau3AI GATC 2 cut(s) 69, 245
SetI ASST 3 cut(s) 43, 47, 69
SfaNI GCATC 1 cut(s) 140
SmiMI CAYNNNNRTG 2 cut(s) 43, 183
Sse9I AATT 3 cut(s) 49, 145, 277
SspMI CTAG 1 cut(s) 227
TaaI ACNGT 1 cut(s) 56
TaiI ACGT 1 cut(s) 47
TaqI TCGA 1 cut(s) 101
TasI AATT 3 cut(s) 49, 145, 277
TatI WGTACW 1 cut(s) 84
TfiI GAWTC 2 cut(s) 10, 103
TseFI GTSAC 1 cut(s) 36
Tsp45I GTSAC 1 cut(s) 36
TspDTI ATGAA 1 cut(s) 201
XceI RCATGY 2 cut(s) 168, 197
XspI CTAG 1 cut(s) 227
Zsp2I ATGCAT 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.