MD11G1077300.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003425
Physical Location & Seq
Reverse (-)
6540713 .. 6542026
1314 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1077300.v1.1.491

Sequence Viewer

Length: 1140 bp
ATGAATCTGTCTTGTTTGAGTGCAAATCTGTTTTCTTTTCGTGGTTCCTGGGTCCTTTGTGAATATATTCAAGGGTTGGCAGAAGTTGCAGAGGGCTTGGGAACAAAACCCTGTGAAGAATTCTGGGCCAAGCTCGTTCCATCAGACTCGAGATATCCGGATGTTGAGATTAGTTCATGTGAGATTGTAGTTTGCTCAGAGATCTTGTTCTCTTCTATTCGCAAATGCAAGTGGTGCAAGATAACAAGGAATTCAGACCACTCTTCTGCCACAATACAAAATAAAAGTTCAGATGCGATAGTTGTTGACAGGACTTTAATCCAAGCTGAAGACACTATTGTTATCCGATGCGGAAGTGAAATTACTTCAGGTCCCGATAAGGAAGGGTATTTAAGCTACAAGTTCAACGTATTGCCTGGTCCTGAAAGTTGTCAGAAGAAGTTAAAGATTTCTATGGATGCCGAGCATACAATATGCTGCATTTACTTGAACATATGGCATGATGTAGTTACCGTTGCTCCTTGCTTCCATAATTTCTGTAACGGGTGCTTCGCAGAGTGGTTAAAGAGATCACTGGAGAAACTTGTTGGAAGAAATCACTTCTTGCTTAACGTTGCGGATGAAATAGTAAAAGTTGATTATTCACTAAAACGCACCAATGATGAAGTGGCAATTTTAGATTCTTATGCAACAATACAATCTAATCTTGTCGTAAACTCTGGAAAACATCTTCGCGGAAAAAGGGCTTGCTCCCAGGTGGATGAAGAAGCTGATAATCCATCAAATCCATGCCCTCAATGTGGTACTGAATATGGTGGGTTCCGCTGCAATCAAACCACTATCCATCTGCAATGTCAAGCATGCGGAGGAATGATGCCTTCACGCACCAATAACAGTGTACCTCAACACTGTTTAGGATGTGATAAGGCATTTTGCAGTACATATTGGCATGCGCAAAGGGTTTCACAAAGTGATTCTCACCGTGTGTGCTGTCGCGAGACTTTTAAATCAATCTCAGCTCATGAAAACAATCGACATGAACAAGTTATCACTGAGAAGTGTATTGCACAATTGGGGAGAACATTGCAGGATGTTATTGCAGAATGGCTTGCAAAGTTGAACAACAGAGAAATTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000166 GO:0000209 GO:0000278 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016604 GO:0016605 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0022402 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031647 GO:0031648 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0036094 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045732 GO:0045786 GO:0045862 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051726 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0097159 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1903047 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-RING_2

Protein Analysis

380

Amino Acids

42.51

Weight (kDa)

6.19

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CRD PF17979 264 - 378 8.3e-31 Cysteine rich domain with multizinc binding regions
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 954
AccII CGCG 2 cut(s) 735, 996
AccIII TCCGGA 1 cut(s) 157
AciI CCGC 5 cut(s) 351, 617, 735, 823, 864
AclI AACGTT 1 cut(s) 612
AcsI RAATTY 2 cut(s) 119, 250
AcuI CTGAAG 2 cut(s) 348, 351
AdeI CACNNNGTG 2 cut(s) 971, 985
AfaI GTAC 3 cut(s) 805, 900, 940
AfiI CCNNNNNNNGG 1 cut(s) 800
AgsI TTSAA 4 cut(s) 71, 406, 490, 1120
AjnI CCWGG 3 cut(s) 47, 415, 753
AluBI AGCT 5 cut(s) 133, 326, 396, 770, 1019
AluI AGCT 5 cut(s) 133, 326, 396, 770, 1019
Alw26I GTCTC 1 cut(s) 992
Ama87I CYCGRG 1 cut(s) 148
Aor13HI TCCGGA 1 cut(s) 157
AoxI GGCC 1 cut(s) 126
ApeKI GCWGC 2 cut(s) 477, 825
ApoI RAATTY 2 cut(s) 119, 250
Asp700I GAANNNNTTC 1 cut(s) 66
AspLEI GCGC 1 cut(s) 955
AspS9I GGNCC 4 cut(s) 52, 126, 371, 419
AsuHPI GGTGA 1 cut(s) 971
AvaI CYCGRG 1 cut(s) 148
AvaII GGWCC 3 cut(s) 52, 371, 419
BarI GAAGNNNNNNTAC 2 cut(s) 346, 378
BbsI GAAGAC 1 cut(s) 336
BbvI GCAGC 2 cut(s) 464, 812
BccI CCATC 3 cut(s) 148, 787, 852
BciT130I CCWGG 3 cut(s) 49, 417, 755
BcoDI GTCTC 1 cut(s) 992
BglII AGATCT 1 cut(s) 201
BisI GCNGC 2 cut(s) 478, 826
BlsI GCNGC 2 cut(s) 479, 827
Bme1390I CCNGG 3 cut(s) 49, 417, 755
Bme18I GGWCC 3 cut(s) 52, 371, 419
BmeT110I CYCGRG 1 cut(s) 148
BmgT120I GGNCC 4 cut(s) 52, 126, 371, 419
BmiI GGNNCC 4 cut(s) 46, 53, 373, 821
BmrFI CCNGG 3 cut(s) 49, 417, 755
BmsI GCATC 4 cut(s) 283, 338, 448, 864
BpiI GAAGAC 1 cut(s) 336
BpmI CTGGAG 1 cut(s) 596
BsaJI CCNNGG 2 cut(s) 48, 753
BsaWI WCCGGW 1 cut(s) 157
BsaXI ACNNNNNCTCC 2 cut(s) 502, 532
Bsc4I CCNNNNNNNGG 1 cut(s) 800
Bse1I ACTGG 1 cut(s) 579
Bse3DI GCAATG 2 cut(s) 857, 1082
BseAI TCCGGA 1 cut(s) 157
BseBI CCWGG 3 cut(s) 49, 417, 755
BseDI CCNNGG 2 cut(s) 48, 753
BseGI GGATG 6 cut(s) 166, 463, 625, 766, 923, 1096
BseLI CCNNNNNNNGG 1 cut(s) 800
BseMI GCAATG 2 cut(s) 857, 1082
BseMII CTCAG 3 cut(s) 210, 1029, 1044
BseNI ACTGG 1 cut(s) 579
BseXI GCAGC 2 cut(s) 464, 812
Bsh1236I CGCG 2 cut(s) 735, 996
BshFI GGCC 1 cut(s) 128
BsiHKCI CYCGRG 1 cut(s) 148
BsiSI CCGG 1 cut(s) 158
BslFI GGGAC 1 cut(s) 357
BslI CCNNNNNNNGG 1 cut(s) 800
BsmAI GTCTC 1 cut(s) 992
BsmFI GGGAC 1 cut(s) 357
BsnI GGCC 1 cut(s) 128
BsoBI CYCGRG 1 cut(s) 148
Bsp13I TCCGGA 1 cut(s) 157
Bsp143I GATC 2 cut(s) 201, 569
Bsp68I TCGCGA 1 cut(s) 996
BspACI CCGC 5 cut(s) 351, 617, 735, 823, 864
BspANI GGCC 1 cut(s) 128
BspCNI CTCAG 3 cut(s) 209, 1028, 1045
BspEI TCCGGA 1 cut(s) 157
BspFNI CGCG 2 cut(s) 735, 996
BspHI TCATGA 1 cut(s) 1021
BspLI GGNNCC 4 cut(s) 46, 53, 373, 821
BsrDI GCAATG 2 cut(s) 857, 1082
BsrI ACTGG 1 cut(s) 579
BssECI CCNNGG 2 cut(s) 48, 753
BssMI GATC 2 cut(s) 201, 569
Bst2UI CCWGG 3 cut(s) 49, 417, 755
Bst4CI ACNGT 4 cut(s) 514, 896, 911, 983
Bst6I CTCTTC 2 cut(s) 217, 268
BstAPI GCANNNNNTGC 2 cut(s) 86, 234
BstC8I GCNNGC 4 cut(s) 748, 862, 951, 1110
BstDEI CTNAG 3 cut(s) 196, 1015, 1053
BstF5I GGATG 6 cut(s) 166, 463, 625, 766, 923, 1096
BstFNI CGCG 2 cut(s) 735, 996
BstHHI GCGC 1 cut(s) 955
BstKTI GATC 2 cut(s) 204, 572
BstMAI GTCTC 1 cut(s) 992
BstMBI GATC 2 cut(s) 201, 569
BstMWI GCNNNNNNNGC 2 cut(s) 86, 234
BstNI CCWGG 3 cut(s) 49, 417, 755
BstNSI RCATGY 2 cut(s) 864, 953
BstSCI CCNGG 3 cut(s) 47, 415, 753
BstUI CGCG 2 cut(s) 735, 996
BstV1I GCAGC 2 cut(s) 464, 812
BstV2I GAAGAC 1 cut(s) 336
BstX2I RGATCY 1 cut(s) 201
BstYI RGATCY 1 cut(s) 201
BsuRI GGCC 1 cut(s) 128
BtsCI GGATG 6 cut(s) 166, 463, 625, 766, 923, 1096
BtsIMutI CAGTG 4 cut(s) 572, 901, 907, 1050
BtuMI TCGCGA 1 cut(s) 996
Cac8I GCNNGC 4 cut(s) 748, 862, 951, 1110
CciI TCATGA 1 cut(s) 1021
CfoI GCGC 1 cut(s) 955
Cfr13I GGNCC 4 cut(s) 52, 126, 371, 419
Csp6I GTAC 3 cut(s) 804, 899, 939
CviAII CATG 7 cut(s) 177, 500, 789, 861, 950, 1022, 1037
CviJI RGCY 9 cut(s) 96, 128, 133, 326, 396, 746, 770, 1019, 1108
CviKI_1 RGCY 9 cut(s) 96, 128, 133, 326, 396, 746, 770, 1019, 1108
CviQI GTAC 3 cut(s) 804, 899, 939
DdeI CTNAG 3 cut(s) 196, 1015, 1053
DpnI GATC 2 cut(s) 203, 571
DpnII GATC 2 cut(s) 201, 569
DraI TTTAAA 1 cut(s) 1006
DraIII CACNNNGTG 2 cut(s) 971, 985
Eam1104I CTCTTC 2 cut(s) 217, 268
EarI CTCTTC 2 cut(s) 217, 268
Eco32I GATATC 1 cut(s) 155
Eco47I GGWCC 3 cut(s) 52, 371, 419
Eco57I CTGAAG 2 cut(s) 348, 351
Eco88I CYCGRG 1 cut(s) 148
EcoO109I RGGNCCY 2 cut(s) 52, 371
EcoRI GAATTC 2 cut(s) 119, 250
EcoRII CCWGG 3 cut(s) 47, 415, 753
EcoRV GATATC 1 cut(s) 155
FaeI CATG 7 cut(s) 180, 503, 792, 864, 953, 1025, 1040
FaqI GGGAC 1 cut(s) 357
FatI CATG 7 cut(s) 176, 499, 788, 860, 949, 1021, 1036
FauNDI CATATG 1 cut(s) 494
Fnu4HI GCNGC 2 cut(s) 478, 826
FokI GGATG 6 cut(s) 173, 470, 632, 773, 930, 1103
Fsp4HI GCNGC 2 cut(s) 478, 826
FspI TGCGCA 1 cut(s) 954
GlaI GCGC 1 cut(s) 954
GluI GCNGC 2 cut(s) 478, 826
GsuI CTGGAG 1 cut(s) 596
HaeIII GGCC 1 cut(s) 128
HapII CCGG 1 cut(s) 158
HhaI GCGC 1 cut(s) 955
Hin1II CATG 7 cut(s) 180, 503, 792, 864, 953, 1025, 1040
Hin6I GCGC 1 cut(s) 953
HinP1I GCGC 1 cut(s) 953
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HinfI GANTC 4 cut(s) 4, 146, 680, 974
HpaII CCGG 1 cut(s) 158
HphI GGTGA 1 cut(s) 971
Hpy166II GTNNAC 3 cut(s) 307, 715, 899
Hpy188I TCNGA 6 cut(s) 145, 199, 256, 292, 347, 435
Hpy188III TCNNGA 7 cut(s) 150, 158, 374, 422, 720, 995, 1022
Hpy8I GTNNAC 3 cut(s) 307, 715, 899
HpyAV CCTTC 2 cut(s) 377, 888
HpyCH4III ACNGT 4 cut(s) 514, 896, 911, 983
HpyCH4IV ACGT 2 cut(s) 408, 612
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 234
HpyF3I CTNAG 3 cut(s) 196, 1015, 1053
HpySE526I ACGT 2 cut(s) 408, 612
Hsp92II CATG 7 cut(s) 180, 503, 792, 864, 953, 1025, 1040
HspAI GCGC 1 cut(s) 953
Kpn2I TCCGGA 1 cut(s) 157
Kzo9I GATC 2 cut(s) 201, 569
LmnI GCTCC 2 cut(s) 523, 755
Lsp1109I GCAGC 2 cut(s) 464, 812
LweI GCATC 4 cut(s) 283, 338, 448, 864
MaeII ACGT 2 cut(s) 408, 612
MaeIII GTNAC 2 cut(s) 508, 539
MalI GATC 2 cut(s) 203, 571
MboI GATC 2 cut(s) 201, 569
MboII GAAGA 8 cut(s) 128, 204, 255, 341, 448, 603, 722, 776
MfeI CAATTG 1 cut(s) 1070
MflI RGATCY 1 cut(s) 201
MluCI AATT 7 cut(s) 119, 250, 360, 532, 672, 1070, 1131
MlyI GAGTC 1 cut(s) 140
MmeI TCCRAC 1 cut(s) 568
MnlI CCTC 4 cut(s) 85, 804, 860, 912
MroI TCCGGA 1 cut(s) 157
MroXI GAANNNNTTC 1 cut(s) 66
MseI TTAA 6 cut(s) 317, 392, 443, 563, 609, 1005
MspA1I CMGCKG 1 cut(s) 825
MspI CCGG 1 cut(s) 158
MspR9I CCNGG 3 cut(s) 49, 417, 755
MunI CAATTG 1 cut(s) 1070
MvaI CCWGG 3 cut(s) 49, 417, 755
MvnI CGCG 2 cut(s) 735, 996
MwoI GCNNNNNNNGC 2 cut(s) 86, 234
NdeI CATATG 1 cut(s) 494
NdeII GATC 2 cut(s) 201, 569
NlaIII CATG 7 cut(s) 180, 503, 792, 864, 953, 1025, 1040
NlaIV GGNNCC 4 cut(s) 46, 53, 373, 821
NmeAIII GCCGAG 1 cut(s) 487
NruI TCGCGA 1 cut(s) 996
NsbI TGCGCA 1 cut(s) 954
NspI RCATGY 2 cut(s) 864, 953
PaeI GCATGC 2 cut(s) 864, 953
PaeR7I CTCGAG 1 cut(s) 148
PagI TCATGA 1 cut(s) 1021
PdmI GAANNNNTTC 1 cut(s) 66
PfeI GAWTC 3 cut(s) 4, 680, 974
PkrI GCNGC 2 cut(s) 479, 827
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
PpuMI RGGWCCY 2 cut(s) 52, 371
Psp1406I AACGTT 1 cut(s) 612
Psp5II RGGWCCY 2 cut(s) 52, 371
Psp6I CCWGG 3 cut(s) 47, 415, 753
PspGI CCWGG 3 cut(s) 47, 415, 753
PspN4I GGNNCC 4 cut(s) 46, 53, 373, 821
PspPI GGNCC 4 cut(s) 52, 126, 371, 419
PspPPI RGGWCCY 2 cut(s) 52, 371
PsuI RGATCY 1 cut(s) 201
RruI TCGCGA 1 cut(s) 996
RsaI GTAC 3 cut(s) 805, 900, 940
RsaNI GTAC 3 cut(s) 804, 899, 939
SaqAI TTAA 6 cut(s) 317, 392, 443, 563, 609, 1005
SatI GCNGC 2 cut(s) 478, 826
Sau3AI GATC 2 cut(s) 201, 569
Sau96I GGNCC 4 cut(s) 52, 126, 371, 419
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 3 cut(s) 49, 417, 755
SfaNI GCATC 4 cut(s) 283, 338, 448, 864
Sfr274I CTCGAG 1 cut(s) 148
SinI GGWCC 3 cut(s) 52, 371, 419
SlaI CTCGAG 1 cut(s) 148
SmlI CTYRAG 1 cut(s) 148
SmoI CTYRAG 1 cut(s) 148
SphI GCATGC 2 cut(s) 864, 953
Sse9I AATT 7 cut(s) 119, 250, 360, 532, 672, 1070, 1131
SsiI CCGC 5 cut(s) 351, 617, 735, 823, 864
StyD4I CCNGG 3 cut(s) 47, 415, 753
TaaI ACNGT 4 cut(s) 514, 896, 911, 983
TaiI ACGT 2 cut(s) 411, 615
TaqI TCGA 2 cut(s) 149, 1033
TasI AATT 7 cut(s) 119, 250, 360, 532, 672, 1070, 1131
TatI WGTACW 1 cut(s) 938
TfiI GAWTC 3 cut(s) 4, 680, 974
Tru1I TTAA 6 cut(s) 317, 392, 443, 563, 609, 1005
Tru9I TTAA 6 cut(s) 317, 392, 443, 563, 609, 1005
TscAI CASTG 4 cut(s) 579, 901, 914, 1057
TseI GCWGC 2 cut(s) 477, 825
TspDTI ATGAA 7 cut(s) 17, 165, 636, 678, 777, 1038, 1053
TspRI CASTG 4 cut(s) 579, 901, 914, 1057
VpaK11BI GGWCC 3 cut(s) 52, 371, 419
XapI RAATTY 2 cut(s) 119, 250
XceI RCATGY 2 cut(s) 864, 953
XcmI CCANNNNNNNNNTGG 1 cut(s) 664
XhoI CTCGAG 1 cut(s) 148
XmnI GAANNNNTTC 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.