RchiOBHm_Chr5g0068971

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003425
Physical Location & Seq
Forward (+)
74921548 .. 74924386
2839 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34437

Sequence Viewer

Length: 1338 bp
ATGGCAGAAGTTGGAGAAAGCTCTGGTGCAAAGCCCTCTGGTGAAATCTGGGCCAAGCTTGTTCCTTCCGACTCGAGATTTCCGGATGTTGAGATCAGAGATCTTGTTCTGACTCAAGATACCCCTCAGAGTCAAGATATTGGTTCAGATGAGAGTTTAGTTTGCTCTCCTATTGACAAATGCGAGTGGTGCAGAATTACGAGGAACCCCGATCAGTCTTCTGCCACTATACAAAATAAGAGTTCAAATGCAATACTTGTTGATGGAGCTGCGATCCAATCTGAAGAGAGTACTGTTATCAGATCTGGAAGTGAAATTATTACAGGTCTTGAGAGAGAAGGGTGTTTAAGCTACAGATTCAATGTGTTGCTTGGTCCTGAAACTTGTCAGAAGATGCTGAAGATTAGTGTGGATGCTGAGCATGCAAAGTGCTGCATTTGTTTAAATATATGGCATGATGCTGTTACCATTGCTCCTTGCTTCCATAACTTCTGCAATGGGTGCTTCTCAGAGTGGTTAAGGAGATCACAGGAGAAGCGTTCAAGTGTACTCTGTCCCCAATGTCGAGCAGTTGTGCAATTTGTTGGAAGAAACCACTTCCTGCATAACATTGCCGATGAAATACTGAATGCGGATTCTTCACTGAGACGCTCCAACGAAGACACTGCAGTTTTAGATTCCTATGCAACAGTACGATCAAATCTGGTAATAAAGTCGGGAAAAGGGCTGCTTGGGAAAAGGGTTCGTTCAGCAGTGCAAGATGAAGCTGATAGTGTAGAGCTTCCCTGCCCTCAATGCAGTAATCAATTTGGTGGGTTTCTCTGCAACCGAAGCACTACCCATCTGCAATGTCAAGCATGTGGAGGAATGATGCCTTCACGAACCAATACAAGTGTACCTCAACACTGTATAGGGTGTGATAGGTCATTTTGCGGTGCATATTGGCATGCTCAAGGTGTTGCACAAAGTGCTTCTCACCACATATGCAGTCGCGATACTTTCAAACCAATCTCAGAACGCACCATCTCTAGAATTCCTACTTCAGCACATGAAAACAATCGCCATGAACAAGAAATCACTATGAGGTGTATTGCACAAATGGGAAGAACATTGCAGGATGTTATTGCAGAATGGATGGTAATCGATTGTTATGAGAAACTGATCTCATTCCTCTTGTACTGGTTTCGGGTTTCAGTTTCTGCATCCCATCTTCCCCCTGATGCATCAAAAAGGGAAAATTGCTGGTATGGATATTCATGTCGAACACAGCACCATAATGAAGACCATGCTCGTAAAAGGAATCATGTATGCCGGCCGACTAGGGGTGCTAATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000166 GO:0000209 GO:0000278 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016604 GO:0016605 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0022402 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031647 GO:0031648 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0036094 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045732 GO:0045786 GO:0045862 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051726 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0097159 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1903047 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-RING_2

Protein Analysis

445

Amino Acids

49.69

Weight (kDa)

6.77

Isoelectric Point (pI)

56.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 143 - 189 3.3e-07 Ring finger domain
zf-C3HC4_2 PF13923 143 - 188 9e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 144 - 188 2.6e-06 Zinc finger, C3HC4 type (RING finger)
zf-CRD PF17979 263 - 379 1.1e-34 Cysteine rich domain with multizinc binding regions
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 993
AccIII TCCGGA 1 cut(s) 82
AciI CCGC 2 cut(s) 632, 933
AclWI GGATC 1 cut(s) 268
AcoI YGGCCR 1 cut(s) 1313
AcsI RAATTY 1 cut(s) 1032
AcuI CTGAAG 3 cut(s) 303, 419, 1026
AdeI CACNNNGTG 1 cut(s) 968
AfaI GTAC 5 cut(s) 292, 549, 693, 897, 1178
AfiI CCNNNNNNNGG 1 cut(s) 1322
AgsI TTSAA 4 cut(s) 246, 361, 543, 1003
AluBI AGCT 6 cut(s) 21, 58, 269, 351, 767, 781
AluI AGCT 6 cut(s) 21, 58, 269, 351, 767, 781
Alw26I GTCTC 1 cut(s) 640
AlwI GGATC 1 cut(s) 268
AlwNI CAGNNNCTG 1 cut(s) 1199
Ama87I CYCGRG 1 cut(s) 73
Aor13HI TCCGGA 1 cut(s) 82
AoxI GGCC 2 cut(s) 51, 1313
ApeKI GCWGC 3 cut(s) 269, 432, 727
ApoI RAATTY 1 cut(s) 1032
AspS9I GGNCC 2 cut(s) 51, 374
AsuHPI GGTGA 2 cut(s) 53, 968
AvaI CYCGRG 1 cut(s) 73
AvaII GGWCC 1 cut(s) 374
BaeI ACNNNNGTAYC 2 cut(s) 879, 912
BbsI GAAGAC 3 cut(s) 210, 666, 1287
BbvI GCAGC 3 cut(s) 256, 419, 714
BccI CCATC 5 cut(s) 257, 849, 1031, 1129, 1215
BcgI CGANNNNNNTGC 2 cut(s) 647, 681
BcoDI GTCTC 1 cut(s) 640
BfaI CTAG 2 cut(s) 1029, 1320
BfmI CTRYAG 2 cut(s) 352, 666
BglII AGATCT 2 cut(s) 100, 302
BisI GCNGC 3 cut(s) 270, 433, 728
BlpI GCTNAGC 1 cut(s) 417
BlsI GCNGC 3 cut(s) 271, 434, 729
BmcAI AGTACT 1 cut(s) 292
Bme18I GGWCC 1 cut(s) 374
BmeT110I CYCGRG 1 cut(s) 73
BmgT120I GGNCC 2 cut(s) 51, 374
BmiI GGNNCC 1 cut(s) 206
BmsI GCATC 7 cut(s) 384, 403, 448, 861, 1210, 1211, 1232
BpiI GAAGAC 3 cut(s) 210, 666, 1287
Bpu1102I GCTNAGC 1 cut(s) 417
BpuEI CTTGAG 3 cut(s) 99, 350, 936
Bsa29I ATCGAT 1 cut(s) 1143
BsaBI GATNNNNATC 1 cut(s) 1139
BsaWI WCCGGW 1 cut(s) 82
BsaXI ACNNNNNCTCC 2 cut(s) 457, 487
Bsc4I CCNNNNNNNGG 1 cut(s) 1322
Bse118I RCCGGY 1 cut(s) 1311
Bse1I ACTGG 1 cut(s) 1184
Bse3DI GCAATG 5 cut(s) 468, 502, 609, 854, 1109
Bse8I GATNNNNATC 1 cut(s) 1139
BseAI TCCGGA 1 cut(s) 82
BseCI ATCGAT 1 cut(s) 1143
BseGI GGATG 5 cut(s) 91, 418, 1123, 1140, 1202
BseJI GATNNNNATC 1 cut(s) 1139
BseLI CCNNNNNNNGG 1 cut(s) 1322
BseMI GCAATG 5 cut(s) 468, 502, 609, 854, 1109
BseMII CTCAG 5 cut(s) 140, 408, 522, 635, 1026
BseNI ACTGG 1 cut(s) 1184
BseX3I CGGCCG 1 cut(s) 1313
BseXI GCAGC 3 cut(s) 256, 419, 714
BsgI GTGCAG 1 cut(s) 211
Bsh1236I CGCG 1 cut(s) 993
Bsh1285I CGRYCG 1 cut(s) 1316
BshFI GGCC 2 cut(s) 53, 1315
BshVI ATCGAT 1 cut(s) 1143
BsiEI CGRYCG 1 cut(s) 1316
BsiHKCI CYCGRG 1 cut(s) 73
BsiSI CCGG 2 cut(s) 83, 1312
BslFI GGGAC 1 cut(s) 540
BslI CCNNNNNNNGG 1 cut(s) 1322
BsmAI GTCTC 1 cut(s) 640
BsmBI CGTCTC 1 cut(s) 640
BsmFI GGGAC 1 cut(s) 540
BsmI GAATGC 1 cut(s) 634
BsnI GGCC 2 cut(s) 53, 1315
BsoBI CYCGRG 1 cut(s) 73
Bsp13I TCCGGA 1 cut(s) 82
Bsp143I GATC 8 cut(s) 93, 100, 211, 273, 302, 524, 695, 1161
Bsp1720I GCTNAGC 1 cut(s) 417
Bsp68I TCGCGA 1 cut(s) 993
BspACI CCGC 2 cut(s) 632, 933
BspANI GGCC 2 cut(s) 53, 1315
BspCNI CTCAG 5 cut(s) 139, 409, 521, 636, 1025
BspDI ATCGAT 1 cut(s) 1143
BspEI TCCGGA 1 cut(s) 82
BspFNI CGCG 1 cut(s) 993
BspLI GGNNCC 1 cut(s) 206
BspMAI CTGCAG 1 cut(s) 670
BspPI GGATC 1 cut(s) 268
BsrDI GCAATG 5 cut(s) 468, 502, 609, 854, 1109
BsrFI RCCGGY 1 cut(s) 1311
BsrI ACTGG 1 cut(s) 1184
BssAI RCCGGY 1 cut(s) 1311
BssMI GATC 8 cut(s) 93, 100, 211, 273, 302, 524, 695, 1161
Bst4CI ACNGT 3 cut(s) 295, 691, 908
Bst6I CTCTTC 1 cut(s) 279
BstAPI GCANNNNNTGC 2 cut(s) 501, 968
BstC8I GCNNGC 3 cut(s) 423, 948, 1313
BstDEI CTNAG 5 cut(s) 126, 417, 508, 644, 1012
BstF5I GGATG 5 cut(s) 91, 418, 1123, 1140, 1202
BstFNI CGCG 1 cut(s) 993
BstKTI GATC 8 cut(s) 96, 103, 214, 276, 305, 527, 698, 1164
BstMAI GTCTC 1 cut(s) 640
BstMBI GATC 8 cut(s) 93, 100, 211, 273, 302, 524, 695, 1161
BstMCI CGRYCG 1 cut(s) 1316
BstMWI GCNNNNNNNGC 6 cut(s) 189, 422, 501, 795, 831, 968
BstNSI RCATGY 3 cut(s) 425, 861, 950
BstSFI CTRYAG 2 cut(s) 352, 666
BstUI CGCG 1 cut(s) 993
BstV1I GCAGC 3 cut(s) 256, 419, 714
BstV2I GAAGAC 3 cut(s) 210, 666, 1287
BstX2I RGATCY 2 cut(s) 100, 302
BstYI RGATCY 2 cut(s) 100, 302
BstZI CGGCCG 1 cut(s) 1313
Bsu15I ATCGAT 1 cut(s) 1143
BsuRI GGCC 2 cut(s) 53, 1315
BsuTUI ATCGAT 1 cut(s) 1143
BtsCI GGATG 5 cut(s) 91, 418, 1123, 1140, 1202
BtsI GCAGTG 2 cut(s) 663, 759
BtsIMutI CAGTG 4 cut(s) 641, 663, 759, 904
BtuMI TCGCGA 1 cut(s) 993
Cac8I GCNNGC 3 cut(s) 423, 948, 1313
CaiI CAGNNNCTG 1 cut(s) 1199
Cfr10I RCCGGY 1 cut(s) 1311
Cfr13I GGNCC 2 cut(s) 51, 374
ClaI ATCGAT 1 cut(s) 1143
CseI GACGC 1 cut(s) 657
Csp6I GTAC 5 cut(s) 291, 548, 692, 896, 1177
CviAII CATG 9 cut(s) 422, 455, 858, 947, 1049, 1064, 1257, 1286, 1304
CviQI GTAC 5 cut(s) 291, 548, 692, 896, 1177
DdeI CTNAG 5 cut(s) 126, 417, 508, 644, 1012
DpnI GATC 8 cut(s) 95, 102, 213, 275, 304, 526, 697, 1163
DpnII GATC 8 cut(s) 93, 100, 211, 273, 302, 524, 695, 1161
DraI TTTAAA 1 cut(s) 444
DraIII CACNNNGTG 1 cut(s) 968
EaeI YGGCCR 1 cut(s) 1313
EagI CGGCCG 1 cut(s) 1313
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
EclXI CGGCCG 1 cut(s) 1313
Eco47I GGWCC 1 cut(s) 374
Eco52I CGGCCG 1 cut(s) 1313
Eco57I CTGAAG 3 cut(s) 303, 419, 1026
Eco88I CYCGRG 1 cut(s) 73
EcoRI GAATTC 1 cut(s) 1032
EcoT22I ATGCAT 1 cut(s) 1225
Esp3I CGTCTC 1 cut(s) 640
FaeI CATG 9 cut(s) 425, 458, 861, 950, 1052, 1067, 1260, 1289, 1307
FalI AAGNNNNNCTT 2 cut(s) 714, 746
FaqI GGGAC 1 cut(s) 540
FatI CATG 9 cut(s) 421, 454, 857, 946, 1048, 1063, 1256, 1285, 1303
FauNDI CATATG 1 cut(s) 983
Fnu4HI GCNGC 3 cut(s) 270, 433, 728
FokI GGATG 5 cut(s) 98, 425, 1130, 1147, 1189
Fsp4HI GCNGC 3 cut(s) 270, 433, 728
FspBI CTAG 2 cut(s) 1029, 1320
GluI GCNGC 3 cut(s) 270, 433, 728
HaeIII GGCC 2 cut(s) 53, 1315
HapII CCGG 2 cut(s) 83, 1312
HgaI GACGC 1 cut(s) 657
Hin1II CATG 9 cut(s) 425, 458, 861, 950, 1052, 1067, 1260, 1289, 1307
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 7 cut(s) 71, 112, 130, 357, 635, 677, 1300
HpaII CCGG 2 cut(s) 83, 1312
HphI GGTGA 2 cut(s) 53, 968
Hpy166II GTNNAC 2 cut(s) 548, 896
Hpy8I GTNNAC 2 cut(s) 548, 896
HpyAV CCTTC 3 cut(s) 75, 332, 885
HpyCH4III ACNGT 3 cut(s) 295, 691, 908
HpyF10VI GCNNNNNNNGC 6 cut(s) 189, 422, 501, 795, 831, 968
HpyF3I CTNAG 5 cut(s) 126, 417, 508, 644, 1012
Hsp92II CATG 9 cut(s) 425, 458, 861, 950, 1052, 1067, 1260, 1289, 1307
Kpn2I TCCGGA 1 cut(s) 82
KroI GCCGGC 1 cut(s) 1311
KroNI GCCGGC 1 cut(s) 1313
Kzo9I GATC 8 cut(s) 93, 100, 211, 273, 302, 524, 695, 1161
LmnI GCTCC 3 cut(s) 266, 478, 656
Lsp1109I GCAGC 3 cut(s) 256, 419, 714
LweI GCATC 7 cut(s) 384, 403, 448, 861, 1210, 1211, 1232
MaeI CTAG 2 cut(s) 1029, 1320
MaeIII GTNAC 1 cut(s) 463
MalI GATC 8 cut(s) 95, 102, 213, 275, 304, 526, 697, 1163
MboI GATC 8 cut(s) 93, 100, 211, 273, 302, 524, 695, 1161
MflI RGATCY 2 cut(s) 100, 302
MluCI AATT 6 cut(s) 195, 315, 578, 806, 1032, 1237
MlyI GAGTC 3 cut(s) 65, 106, 139
MmeI TCCRAC 3 cut(s) 93, 565, 678
MnlI CCTC 8 cut(s) 46, 135, 195, 801, 857, 909, 1077, 1181
Mph1103I ATGCAT 1 cut(s) 1225
MroI TCCGGA 1 cut(s) 82
MroNI GCCGGC 1 cut(s) 1311
MseI TTAA 3 cut(s) 347, 443, 518
MslI CAYNNNNRTG 1 cut(s) 1275
MspI CCGG 2 cut(s) 83, 1312
Mva1269I GAATGC 1 cut(s) 634
MvnI CGCG 1 cut(s) 993
MwoI GCNNNNNNNGC 6 cut(s) 189, 422, 501, 795, 831, 968
NaeI GCCGGC 1 cut(s) 1313
NdeI CATATG 1 cut(s) 983
NdeII GATC 8 cut(s) 93, 100, 211, 273, 302, 524, 695, 1161
NgoMIV GCCGGC 1 cut(s) 1311
NlaIII CATG 9 cut(s) 425, 458, 861, 950, 1052, 1067, 1260, 1289, 1307
NlaIV GGNNCC 1 cut(s) 206
NruI TCGCGA 1 cut(s) 993
NsiI ATGCAT 1 cut(s) 1225
NspI RCATGY 3 cut(s) 425, 861, 950
PaeI GCATGC 2 cut(s) 425, 950
PaeR7I CTCGAG 1 cut(s) 73
PctI GAATGC 1 cut(s) 634
PdiI GCCGGC 1 cut(s) 1313
PfeI GAWTC 4 cut(s) 357, 635, 677, 1300
PkrI GCNGC 3 cut(s) 271, 434, 729
PleI GAGTC 3 cut(s) 65, 106, 138
PpsI GAGTC 3 cut(s) 65, 106, 138
PspN4I GGNNCC 1 cut(s) 206
PspPI GGNCC 2 cut(s) 51, 374
PstI CTGCAG 1 cut(s) 670
PstNI CAGNNNCTG 1 cut(s) 1199
PsuI RGATCY 2 cut(s) 100, 302
RruI TCGCGA 1 cut(s) 993
RsaI GTAC 5 cut(s) 292, 549, 693, 897, 1178
RsaNI GTAC 5 cut(s) 291, 548, 692, 896, 1177
RseI CAYNNNNRTG 1 cut(s) 1275
SaqAI TTAA 3 cut(s) 347, 443, 518
SatI GCNGC 3 cut(s) 270, 433, 728
Sau3AI GATC 8 cut(s) 93, 100, 211, 273, 302, 524, 695, 1161
Sau96I GGNCC 2 cut(s) 51, 374
ScaI AGTACT 1 cut(s) 292
SchI GAGTC 3 cut(s) 65, 106, 139
SfaNI GCATC 7 cut(s) 384, 403, 448, 861, 1210, 1211, 1232
SfcI CTRYAG 2 cut(s) 352, 666
Sfr274I CTCGAG 1 cut(s) 73
SinI GGWCC 1 cut(s) 374
SlaI CTCGAG 1 cut(s) 73
SmiMI CAYNNNNRTG 1 cut(s) 1275
SmlI CTYRAG 4 cut(s) 73, 114, 329, 951
SmoI CTYRAG 4 cut(s) 73, 114, 329, 951
SphI GCATGC 2 cut(s) 425, 950
Sse9I AATT 6 cut(s) 195, 315, 578, 806, 1032, 1237
SsiI CCGC 2 cut(s) 632, 933
SspMI CTAG 2 cut(s) 1029, 1320
TaaI ACNGT 3 cut(s) 295, 691, 908
TaqI TCGA 4 cut(s) 74, 565, 1143, 1261
TasI AATT 6 cut(s) 195, 315, 578, 806, 1032, 1237
TatI WGTACW 3 cut(s) 290, 547, 1176
TfiI GAWTC 4 cut(s) 357, 635, 677, 1300
Tru1I TTAA 3 cut(s) 347, 443, 518
Tru9I TTAA 3 cut(s) 347, 443, 518
TscAI CASTG 4 cut(s) 648, 670, 759, 911
TseI GCWGC 3 cut(s) 269, 432, 727
TspDTI ATGAA 6 cut(s) 633, 777, 1065, 1080, 1245, 1293
TspRI CASTG 4 cut(s) 648, 670, 759, 911
VpaK11BI GGWCC 1 cut(s) 374
XapI RAATTY 1 cut(s) 1032
XbaI TCTAGA 1 cut(s) 1028
XceI RCATGY 3 cut(s) 425, 861, 950
XhoI CTCGAG 1 cut(s) 73
XspI CTAG 2 cut(s) 1029, 1320
ZrmI AGTACT 1 cut(s) 292
Zsp2I ATGCAT 1 cut(s) 1225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.