AT1G54110

Membrane fusion protein Use1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003166
Physical Location & Seq
Forward (+)
20199310 .. 20201787
2478 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G54110.2

Sequence Viewer

Length: 711 bp
ATGGGAATCGGCAAAACGGAAATCAATTTCATGAGATTACTTTCAGCTGCACCTAATCAGCAAAACCAGTCTAAGCTTATGCATTATGTTGCTACTTTGAGGGAACAGTTGGAACAACTCTCGGAAGAGAAGACCCTTGAAGGGCTTCCCAGAGTTACAAACGCTAAGGTGAATGAGTACTATGAGAAGATTGAAGCTGTTGTTTCACGAATAGTTGCTCAAGTGCCTCACACAGAGGTATCTGATGAAGCTTTTGCAAAGGATTCTACTAACGATAGTTCTCCCAAAGTAGAAGACGATACACGAACCCCCAATTCTCCACAGCTGAGAAGAAGAATCGTGCCTGCAAGTTCCAAAGAGCAAAGCTATGACGCTGATCCCTCAAAACCAATAAAACTAGACACCGCAGCTCAAGCGCAAGTTAACAAGCAGAGAAAGCTTCAAGAGGATCTAACCGATGAAATGGTGGTACTTGCGAGACAACTTAAGGAGAGAAGTCAAATGATAAGCCAATCTGTGCAAAATACAGAAAAGATACTTGATTCTACCGAGGAAGCCATTGAGCAAAGCTTAGCAAGCACAGGACACGCAACCGTAAGAGCCACAAAGATTTATTCAGAAAGCTCAAAGACGAGTTGCTTCCAGTGGCTCTTGATCCTCGCCATGACATGTGTGTTCATCATGGTTGTCATGTTGATCCGAGTCACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 map04130
Pfam Domains
Protein Families
Pcc1 Use1

Protein Analysis

236

Amino Acids

26.56

Weight (kDa)

5.76

Isoelectric Point (pI)

62.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Use1 PF09753 37 - 244 7.5e-51 Membrane fusion protein Use1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 405
AclWI GGATC 4 cut(s) 371, 456, 649, 691
AfaI GTAC 2 cut(s) 179, 471
AfiI CCNNNNNNNGG 1 cut(s) 141
AflII CTTAAG 1 cut(s) 485
AflIII ACRYGT 1 cut(s) 668
AgsI TTSAA 3 cut(s) 140, 194, 443
Alw26I GTCTC 1 cut(s) 472
AlwI GGATC 4 cut(s) 371, 456, 649, 691
ApeKI GCWGC 2 cut(s) 47, 407
Asp700I GAANNNNTTC 1 cut(s) 144
AspLEI GCGC 1 cut(s) 418
AsuHPI GGTGA 1 cut(s) 181
BaeI ACNNNNGTAYC 2 cut(s) 222, 255
BbsI GAAGAC 2 cut(s) 137, 300
BbvI GCAGC 2 cut(s) 34, 419
BcoDI GTCTC 1 cut(s) 472
BfaI CTAG 1 cut(s) 398
BfrI CTTAAG 1 cut(s) 485
BisI GCNGC 2 cut(s) 48, 408
BlpI GCTNAGC 1 cut(s) 571
BlsI GCNGC 2 cut(s) 49, 409
BmcAI AGTACT 1 cut(s) 179
BpiI GAAGAC 2 cut(s) 137, 300
Bpu10I CCTNAGC 1 cut(s) 165
Bpu1102I GCTNAGC 1 cut(s) 571
BpuEI CTTGAG 2 cut(s) 204, 396
BsaJI CCNNGG 1 cut(s) 549
Bsc4I CCNNNNNNNGG 1 cut(s) 141
Bse1I ACTGG 2 cut(s) 67, 643
BseDI CCNNGG 1 cut(s) 549
BseLI CCNNNNNNNGG 1 cut(s) 141
BseMII CTCAG 1 cut(s) 317
BseNI ACTGG 2 cut(s) 67, 643
BseXI GCAGC 2 cut(s) 34, 419
BsgI GTGCAG 1 cut(s) 33
BslI CCNNNNNNNGG 1 cut(s) 141
BsmAI GTCTC 1 cut(s) 472
Bsp143I GATC 4 cut(s) 376, 448, 654, 696
Bsp1720I GCTNAGC 1 cut(s) 571
BspACI CCGC 1 cut(s) 405
BspCNI CTCAG 1 cut(s) 318
BspHI TCATGA 1 cut(s) 30
BspPI GGATC 4 cut(s) 371, 456, 649, 691
BspTI CTTAAG 1 cut(s) 485
BsrI ACTGG 2 cut(s) 67, 643
BssECI CCNNGG 1 cut(s) 549
BssMI GATC 4 cut(s) 376, 448, 654, 696
Bst4CI ACNGT 2 cut(s) 108, 595
Bst6I CTCTTC 1 cut(s) 120
BstAFI CTTAAG 1 cut(s) 485
BstC8I GCNNGC 2 cut(s) 345, 577
BstDEI CTNAG 4 cut(s) 72, 165, 326, 571
BstHHI GCGC 1 cut(s) 418
BstKTI GATC 4 cut(s) 379, 451, 657, 699
BstMAI GTCTC 1 cut(s) 472
BstMBI GATC 4 cut(s) 376, 448, 654, 696
BstMWI GCNNNNNNNGC 3 cut(s) 413, 436, 576
BstNSI RCATGY 1 cut(s) 672
BstV1I GCAGC 2 cut(s) 34, 419
BstV2I GAAGAC 2 cut(s) 137, 300
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
BtsIMutI CAGTG 1 cut(s) 650
Cac8I GCNNGC 2 cut(s) 345, 577
CciI TCATGA 1 cut(s) 30
CfoI GCGC 1 cut(s) 418
CseI GACGC 1 cut(s) 380
Csp6I GTAC 2 cut(s) 178, 470
CviAII CATG 5 cut(s) 31, 664, 669, 682, 691
CviQI GTAC 2 cut(s) 178, 470
DdeI CTNAG 4 cut(s) 72, 165, 326, 571
DpnI GATC 4 cut(s) 378, 450, 656, 698
DpnII GATC 4 cut(s) 376, 448, 654, 696
Eam1104I CTCTTC 1 cut(s) 120
EarI CTCTTC 1 cut(s) 120
EcoT22I ATGCAT 1 cut(s) 84
FaeI CATG 5 cut(s) 34, 667, 672, 685, 694
FatI CATG 5 cut(s) 30, 663, 668, 681, 690
Fnu4HI GCNGC 2 cut(s) 48, 408
Fsp4HI GCNGC 2 cut(s) 48, 408
FspBI CTAG 1 cut(s) 398
GlaI GCGC 1 cut(s) 417
GluI GCNGC 2 cut(s) 48, 408
HgaI GACGC 1 cut(s) 380
HhaI GCGC 1 cut(s) 418
Hin1II CATG 5 cut(s) 34, 667, 672, 685, 694
Hin6I GCGC 1 cut(s) 416
HinP1I GCGC 1 cut(s) 416
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HindIII AAGCTT 4 cut(s) 74, 249, 437, 568
HinfI GANTC 5 cut(s) 6, 263, 336, 542, 702
HpaI GTTAAC 1 cut(s) 424
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 1 cut(s) 424
Hpy188I TCNGA 4 cut(s) 124, 244, 619, 701
Hpy188III TCNNGA 4 cut(s) 31, 207, 443, 652
Hpy8I GTNNAC 1 cut(s) 424
HpyAV CCTTC 1 cut(s) 134
HpyCH4III ACNGT 2 cut(s) 108, 595
HpyCH4V TGCA 5 cut(s) 50, 82, 257, 347, 520
HpyF10VI GCNNNNNNNGC 3 cut(s) 413, 436, 576
HpyF3I CTNAG 4 cut(s) 72, 165, 326, 571
Hsp92II CATG 5 cut(s) 34, 667, 672, 685, 694
HspAI GCGC 1 cut(s) 416
KspAI GTTAAC 1 cut(s) 424
Kzo9I GATC 4 cut(s) 376, 448, 654, 696
LpnPI CCDG 5 cut(s) 80, 163, 357, 567, 656
Lsp1109I GCAGC 2 cut(s) 34, 419
MaeI CTAG 1 cut(s) 398
MaeIII GTNAC 2 cut(s) 154, 703
MalI GATC 4 cut(s) 378, 450, 656, 698
MboI GATC 4 cut(s) 376, 448, 654, 696
MboII GAAGA 6 cut(s) 137, 142, 199, 305, 342, 345
MflI RGATCY 1 cut(s) 448
MluCI AATT 2 cut(s) 25, 313
MlyI GAGTC 1 cut(s) 711
MmeI TCCRAC 1 cut(s) 90
MnlI CCTC 7 cut(s) 93, 229, 237, 391, 439, 544, 668
Mph1103I ATGCAT 1 cut(s) 84
MroXI GAANNNNTTC 1 cut(s) 144
MseI TTAA 2 cut(s) 423, 486
MspA1I CMGCKG 2 cut(s) 47, 325
MspCI CTTAAG 1 cut(s) 485
MwoI GCNNNNNNNGC 3 cut(s) 413, 436, 576
NdeII GATC 4 cut(s) 376, 448, 654, 696
NlaIII CATG 5 cut(s) 34, 667, 672, 685, 694
NmuCI GTSAC 1 cut(s) 703
NsiI ATGCAT 1 cut(s) 84
NspI RCATGY 1 cut(s) 672
PagI TCATGA 1 cut(s) 30
PciI ACATGT 1 cut(s) 668
PdmI GAANNNNTTC 1 cut(s) 144
PfeI GAWTC 4 cut(s) 6, 263, 336, 542
PkrI GCNGC 2 cut(s) 49, 409
PleI GAGTC 1 cut(s) 710
PpsI GAGTC 1 cut(s) 710
PscI ACATGT 1 cut(s) 668
PsuI RGATCY 1 cut(s) 448
PvuII CAGCTG 2 cut(s) 47, 325
RsaI GTAC 2 cut(s) 179, 471
RsaNI GTAC 2 cut(s) 178, 470
SaqAI TTAA 2 cut(s) 423, 486
SatI GCNGC 2 cut(s) 48, 408
Sau3AI GATC 4 cut(s) 376, 448, 654, 696
ScaI AGTACT 1 cut(s) 179
SchI GAGTC 1 cut(s) 711
SmlI CTYRAG 3 cut(s) 219, 411, 485
SmoI CTYRAG 3 cut(s) 219, 411, 485
Sse9I AATT 2 cut(s) 25, 313
SsiI CCGC 1 cut(s) 405
SspMI CTAG 1 cut(s) 398
TaaI ACNGT 2 cut(s) 108, 595
TasI AATT 2 cut(s) 25, 313
TatI WGTACW 1 cut(s) 177
TfiI GAWTC 4 cut(s) 6, 263, 336, 542
Tru1I TTAA 2 cut(s) 423, 486
Tru9I TTAA 2 cut(s) 423, 486
TscAI CASTG 1 cut(s) 650
TseFI GTSAC 1 cut(s) 703
TseI GCWGC 2 cut(s) 47, 407
Tsp45I GTSAC 1 cut(s) 703
TspDTI ATGAA 4 cut(s) 19, 261, 474, 667
TspGWI ACGGA 1 cut(s) 32
TspRI CASTG 1 cut(s) 650
Vha464I CTTAAG 1 cut(s) 485
XceI RCATGY 1 cut(s) 672
XmnI GAANNNNTTC 1 cut(s) 144
XspI CTAG 1 cut(s) 398
ZrmI AGTACT 1 cut(s) 179
Zsp2I ATGCAT 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.