AT3G55600

Membrane fusion protein Use1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0003166
Physical Location & Seq
Forward (+)
20620410 .. 20622555
2146 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G55600.2

Sequence Viewer

Length: 723 bp
ATGATGGGAATAAGCAAAACGGAGATCAATTTGCGGAGATTGCTTTCCGCTGCACCTAACCAGCAAAATCAGTCGAAGCTTATGCATTATGTTGCTACATTGAGGGAACAATTAGAACAACTCTCCGAAGAAAAAACCCCTGAAGGCCTTCCCAGAGTTACAAAGGCTAAGGTTAATGAGTACTATGAGAAGATTGAAGCTGTTGCTTCCAAGATAGCTTCTCAAGAGCCTGAGACAGAGGTATCTGATGAACCCTTTGCAAAAGATTCGACTAGTGGAAGCTCACCAAAAATAGAAGATGAGCCTCGAAGCCCCACTTCGCCACAGCTGAGAAGAAGAATCGTCCCTGCAAGTTCCAAGGAGCAAAGCTTTGATGCTGCTGATGCTGATTCATCAAAACCAATAAAACTAGACACCGCAGCTCAAGCGCACATTGACAAGCACAGAAAGCTTCAAGAGGATCTAACCGATGAAATGGTGGTACTTGCAAGACAACTCAAGGAGAGAAGTCAAGCGATTAGTCAATCTGTGCAAAATACAGAAAAGATACTTGACTCTACCGAGGAGGCCATTGAGCAAAGCCTAGCGAGCACAGGACACGCGACAACAAGAGCGACAAAGATATATTCACAAAGCTCAAAGACAAGTTGCTTCCAGTGGCTCTTGATCTTCGCCATGATCTGTGTGTTCATAATGGTTGTTCTGTTGATCCGGGTCACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 map04130
Pfam Domains
Protein Families
Pcc1 Use1

Protein Analysis

240

Amino Acids

26.84

Weight (kDa)

5.91

Isoelectric Point (pI)

77.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Use1 PF09753 6 - 240 2.3e-68 Membrane fusion protein Use1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 602
AciI CCGC 3 cut(s) 34, 48, 417
AclWI GGATC 2 cut(s) 468, 703
AcuI CTGAAG 1 cut(s) 162
AfaI GTAC 2 cut(s) 182, 483
AgsI TTSAA 2 cut(s) 197, 455
AhlI ACTAGT 1 cut(s) 272
AluBI AGCT 9 cut(s) 79, 200, 218, 282, 328, 369, 422, 451, 636
AluI AGCT 9 cut(s) 79, 200, 218, 282, 328, 369, 422, 451, 636
Alw21I GWGCWC 1 cut(s) 593
Alw26I GTCTC 1 cut(s) 227
AlwI GGATC 2 cut(s) 468, 703
AoxI GGCC 2 cut(s) 145, 567
ApeKI GCWGC 3 cut(s) 50, 377, 419
Asp700I GAANNNNTTC 1 cut(s) 147
AspLEI GCGC 1 cut(s) 430
AsuC2I CCSGG 1 cut(s) 713
AsuHPI GGTGA 1 cut(s) 276
BaeI ACNNNNGTAYC 2 cut(s) 225, 258
Bbv12I GWGCWC 1 cut(s) 593
BbvI GCAGC 3 cut(s) 37, 364, 431
BcgI CGANNNNNNTGC 4 cut(s) 64, 98, 249, 283
BcnI CCSGG 1 cut(s) 713
BcoDI GTCTC 1 cut(s) 227
BcuI ACTAGT 1 cut(s) 272
BfaI CTAG 3 cut(s) 273, 410, 584
BisI GCNGC 3 cut(s) 51, 378, 420
BlsI GCNGC 3 cut(s) 52, 379, 421
BmcAI AGTACT 1 cut(s) 182
Bme1390I CCNGG 1 cut(s) 713
BmrFI CCNGG 1 cut(s) 713
BmsI GCATC 2 cut(s) 364, 373
Bpu10I CCTNAGC 1 cut(s) 168
BpuEI CTTGAG 3 cut(s) 207, 408, 482
BpuMI CCSGG 1 cut(s) 713
BsaJI CCNNGG 2 cut(s) 357, 561
Bse1I ACTGG 1 cut(s) 655
BseDI CCNNGG 2 cut(s) 357, 561
BseMII CTCAG 2 cut(s) 222, 320
BseNI ACTGG 1 cut(s) 655
BseRI GAGGAG 1 cut(s) 578
BseXI GCAGC 3 cut(s) 37, 364, 431
BsgI GTGCAG 1 cut(s) 36
Bsh1236I CGCG 1 cut(s) 602
BshFI GGCC 2 cut(s) 147, 569
BsiHKAI GWGCWC 1 cut(s) 593
BsiSI CCGG 1 cut(s) 712
BslFI GGGAC 1 cut(s) 329
BsmAI GTCTC 1 cut(s) 227
BsmFI GGGAC 1 cut(s) 329
BsnI GGCC 2 cut(s) 147, 569
Bsp1286I GDGCHC 1 cut(s) 593
Bsp143I GATC 5 cut(s) 24, 460, 666, 678, 708
BspACI CCGC 3 cut(s) 34, 48, 417
BspANI GGCC 2 cut(s) 147, 569
BspCNI CTCAG 2 cut(s) 223, 321
BspFNI CGCG 1 cut(s) 602
BspPI GGATC 2 cut(s) 468, 703
BsrI ACTGG 1 cut(s) 655
BssECI CCNNGG 2 cut(s) 357, 561
BssMI GATC 5 cut(s) 24, 460, 666, 678, 708
BssT1I CCWWGG 1 cut(s) 357
BstC8I GCNNGC 1 cut(s) 589
BstDEI CTNAG 3 cut(s) 168, 231, 329
BstFNI CGCG 1 cut(s) 602
BstHHI GCGC 1 cut(s) 430
BstKTI GATC 5 cut(s) 27, 463, 669, 681, 711
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 5 cut(s) 24, 460, 666, 678, 708
BstMWI GCNNNNNNNGC 5 cut(s) 40, 383, 425, 448, 588
BstSCI CCNGG 1 cut(s) 711
BstUI CGCG 1 cut(s) 602
BstV1I GCAGC 3 cut(s) 37, 364, 431
BstX2I RGATCY 1 cut(s) 460
BstYI RGATCY 1 cut(s) 460
BsuRI GGCC 2 cut(s) 147, 569
BtsIMutI CAGTG 1 cut(s) 662
Cac8I GCNNGC 1 cut(s) 589
CfoI GCGC 1 cut(s) 430
Csp6I GTAC 2 cut(s) 181, 482
CviAII CATG 1 cut(s) 676
CviQI GTAC 2 cut(s) 181, 482
DdeI CTNAG 3 cut(s) 168, 231, 329
DpnI GATC 5 cut(s) 26, 462, 668, 680, 710
DpnII GATC 5 cut(s) 24, 460, 666, 678, 708
Eco130I CCWWGG 1 cut(s) 357
Eco147I AGGCCT 1 cut(s) 147
Eco57I CTGAAG 1 cut(s) 162
EcoT14I CCWWGG 1 cut(s) 357
EcoT22I ATGCAT 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 357
FaeI CATG 1 cut(s) 679
FaiI YATR 7 cut(s) 83, 90, 186, 625, 677, 692, 721
FalI AAGNNNNNCTT 2 cut(s) 301, 333
FaqI GGGAC 1 cut(s) 329
FatI CATG 1 cut(s) 675
Fnu4HI GCNGC 3 cut(s) 51, 378, 420
Fsp4HI GCNGC 3 cut(s) 51, 378, 420
FspBI CTAG 3 cut(s) 273, 410, 584
GlaI GCGC 1 cut(s) 429
GluI GCNGC 3 cut(s) 51, 378, 420
HaeIII GGCC 2 cut(s) 147, 569
HapII CCGG 1 cut(s) 712
HhaI GCGC 1 cut(s) 430
Hin1II CATG 1 cut(s) 679
Hin6I GCGC 1 cut(s) 428
HinP1I GCGC 1 cut(s) 428
HindIII AAGCTT 3 cut(s) 77, 367, 449
HinfI GANTC 4 cut(s) 266, 339, 389, 554
HpaII CCGG 1 cut(s) 712
HphI GGTGA 1 cut(s) 276
Hpy188I TCNGA 2 cut(s) 127, 247
Hpy188III TCNNGA 3 cut(s) 224, 455, 664
HpyAV CCTTC 2 cut(s) 137, 158
HpyCH4V TGCA 6 cut(s) 53, 85, 260, 350, 488, 532
HpyF10VI GCNNNNNNNGC 5 cut(s) 40, 383, 425, 448, 588
HpyF3I CTNAG 3 cut(s) 168, 231, 329
Hsp92II CATG 1 cut(s) 679
HspAI GCGC 1 cut(s) 428
Kzo9I GATC 5 cut(s) 24, 460, 666, 678, 708
LmnI GCTCC 1 cut(s) 361
LpnPI CCDG 7 cut(s) 74, 153, 166, 243, 360, 579, 668
Lsp1109I GCAGC 3 cut(s) 37, 364, 431
LweI GCATC 2 cut(s) 364, 373
MaeI CTAG 3 cut(s) 273, 410, 584
MaeIII GTNAC 2 cut(s) 157, 715
MalI GATC 5 cut(s) 26, 462, 668, 680, 710
MboI GATC 5 cut(s) 24, 460, 666, 678, 708
MboII GAAGA 6 cut(s) 140, 202, 308, 345, 348, 661
MflI RGATCY 1 cut(s) 460
MhlI GDGCHC 1 cut(s) 593
MluCI AATT 2 cut(s) 28, 110
MlyI GAGTC 1 cut(s) 548
MnlI CCTC 6 cut(s) 96, 232, 315, 451, 556, 559
Mph1103I ATGCAT 1 cut(s) 87
MroXI GAANNNNTTC 1 cut(s) 147
MseI TTAA 1 cut(s) 174
MspA1I CMGCKG 2 cut(s) 50, 328
MspI CCGG 1 cut(s) 712
MspR9I CCNGG 1 cut(s) 713
MvnI CGCG 1 cut(s) 602
MwoI GCNNNNNNNGC 5 cut(s) 40, 383, 425, 448, 588
NciI CCSGG 1 cut(s) 713
NdeII GATC 5 cut(s) 24, 460, 666, 678, 708
NlaIII CATG 1 cut(s) 679
NmuCI GTSAC 1 cut(s) 715
NsiI ATGCAT 1 cut(s) 87
PceI AGGCCT 1 cut(s) 147
PdmI GAANNNNTTC 1 cut(s) 147
PfeI GAWTC 3 cut(s) 266, 339, 389
PkrI GCNGC 3 cut(s) 52, 379, 421
PleI GAGTC 1 cut(s) 548
PpsI GAGTC 1 cut(s) 548
PsuI RGATCY 1 cut(s) 460
PvuII CAGCTG 1 cut(s) 328
RsaI GTAC 2 cut(s) 182, 483
RsaNI GTAC 2 cut(s) 181, 482
SaqAI TTAA 1 cut(s) 174
SatI GCNGC 3 cut(s) 51, 378, 420
Sau3AI GATC 5 cut(s) 24, 460, 666, 678, 708
ScaI AGTACT 1 cut(s) 182
SchI GAGTC 1 cut(s) 548
ScrFI CCNGG 1 cut(s) 713
SduI GDGCHC 1 cut(s) 593
SfaNI GCATC 2 cut(s) 364, 373
SmlI CTYRAG 3 cut(s) 222, 423, 497
SmoI CTYRAG 3 cut(s) 222, 423, 497
SpeI ACTAGT 1 cut(s) 272
Sse9I AATT 2 cut(s) 28, 110
SseBI AGGCCT 1 cut(s) 147
SsiI CCGC 3 cut(s) 34, 48, 417
SspMI CTAG 3 cut(s) 273, 410, 584
StuI AGGCCT 1 cut(s) 147
StyD4I CCNGG 1 cut(s) 711
StyI CCWWGG 1 cut(s) 357
TaqI TCGA 3 cut(s) 74, 269, 307
TasI AATT 2 cut(s) 28, 110
TatI WGTACW 1 cut(s) 180
TfiI GAWTC 3 cut(s) 266, 339, 389
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TscAI CASTG 1 cut(s) 662
TseFI GTSAC 1 cut(s) 715
TseI GCWGC 3 cut(s) 50, 377, 419
Tsp45I GTSAC 1 cut(s) 715
TspDTI ATGAA 4 cut(s) 264, 381, 486, 679
TspGWI ACGGA 1 cut(s) 35
TspRI CASTG 1 cut(s) 662
XmnI GAANNNNTTC 1 cut(s) 147
XspI CTAG 3 cut(s) 273, 410, 584
ZrmI AGTACT 1 cut(s) 182
Zsp2I ATGCAT 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.