Rroxscaffold_2G00084380

Vesicle transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003166
Physical Location & Seq
Forward (+)
6876419 .. 6876928
510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00084380.1

Sequence Viewer

Length: 510 bp
ATGCAAAACTTTCGCATTATGTTGCTACTTTATGAGAACATGCAGGTAGAACAACTAGCTGAAGAGAGAACATCGGAAGGCTTACCAAGGGTTTCCAAGGCTGTTTTGAGTGACTATTCAGAAAATATCGAAGCCATTGCTTCGAAATTAGCTGCTTCGTTGCCTAATTTGCAAGAACACCACGAGTCCCTTGCAAGAATTTTGGTCAAAGCAAAGTCTTCTGAAACAGGAGACAATCAGGGTCCCCTTTCTCCTGGTCTAAGGAGGACATTTGTGCCCAACTCAAATATCCAAGATGGAACTCGTGAGACTCTTGGTGCAGATTCTTCGTCGCCTGTCAAGCCTGACGCCACAGCCCAAGTGCACATGGAAAAGCACAGAAGACTTCAGGAGGATTTGACTGATGAAATGGTTGGATTGGCAAGGCAACTCAAAGAGACTAGTCTCATGATGAGCCACTCCGTGCAAAACACTGAAAAACTACTTGACTCTACCCGAGGAGGCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 map04130
Pfam Domains
Protein Families
Use1

Protein Analysis

169

Amino Acids

18.73

Weight (kDa)

5.56

Isoelectric Point (pI)

53.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Use1 PF09753 14 - 166 7.1e-29 Membrane fusion protein Use1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 34
AcsI RAATTY 1 cut(s) 198
AcuI CTGAAG 2 cut(s) 81, 371
AcyI GRCGYC 1 cut(s) 348
AdeI CACNNNGTG 1 cut(s) 463
AhlI ACTAGT 1 cut(s) 440
AjnI CCWGG 1 cut(s) 253
AluBI AGCT 2 cut(s) 59, 152
AluI AGCT 2 cut(s) 59, 152
Alw21I GWGCWC 1 cut(s) 366
Alw26I GTCTC 4 cut(s) 225, 302, 431, 449
Alw44I GTGCAC 1 cut(s) 362
Ama87I CYCGRG 1 cut(s) 495
ApaLI GTGCAC 1 cut(s) 362
ApeKI GCWGC 1 cut(s) 152
ApoI RAATTY 1 cut(s) 198
AspS9I GGNCC 1 cut(s) 242
AsuII TTCGAA 1 cut(s) 143
AvaI CYCGRG 1 cut(s) 495
AvaII GGWCC 1 cut(s) 242
BaeGI GKGCMC 2 cut(s) 279, 366
BauI CACGAG 2 cut(s) 182, 303
BbsI GAAGAC 2 cut(s) 210, 388
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 1 cut(s) 139
BccI CCATC 1 cut(s) 290
BcgI CGANNNNNNTGC 7 cut(s) 27, 119, 153, 173, 207, 309, 343
BciT130I CCWGG 1 cut(s) 255
BcoDI GTCTC 4 cut(s) 225, 302, 431, 449
BcuI ACTAGT 1 cut(s) 440
BfaI CTAG 2 cut(s) 56, 441
BfuAI ACCTGC 1 cut(s) 34
BisI GCNGC 1 cut(s) 153
BlsI GCNGC 1 cut(s) 154
Bme1390I CCNGG 1 cut(s) 255
Bme18I GGWCC 1 cut(s) 242
BmeT110I CYCGRG 1 cut(s) 495
BmgT120I GGNCC 1 cut(s) 242
BmiI GGNNCC 2 cut(s) 243, 244
BmrFI CCNGG 1 cut(s) 255
BpiI GAAGAC 2 cut(s) 210, 388
Bpu14I TTCGAA 1 cut(s) 143
BsaHI GRCGYC 1 cut(s) 348
BsaJI CCNNGG 3 cut(s) 86, 96, 496
Bse3DI GCAATG 1 cut(s) 135
BseBI CCWGG 1 cut(s) 255
BseDI CCNNGG 3 cut(s) 86, 96, 496
BseMI GCAATG 1 cut(s) 135
BseSI GKGCMC 2 cut(s) 279, 366
BseXI GCAGC 1 cut(s) 139
BsgI GTGCAG 1 cut(s) 339
BsiHKAI GWGCWC 1 cut(s) 366
BsiHKCI CYCGRG 1 cut(s) 495
BslFI GGGAC 2 cut(s) 172, 228
BsmAI GTCTC 4 cut(s) 225, 302, 431, 449
BsmFI GGGAC 2 cut(s) 172, 228
BsoBI CYCGRG 1 cut(s) 495
Bsp119I TTCGAA 1 cut(s) 143
Bsp1286I GDGCHC 2 cut(s) 279, 366
BspHI TCATGA 1 cut(s) 447
BspLI GGNNCC 2 cut(s) 243, 244
BspMI ACCTGC 1 cut(s) 34
BspT104I TTCGAA 1 cut(s) 143
BsrDI GCAATG 1 cut(s) 135
BssECI CCNNGG 3 cut(s) 86, 96, 496
BssNI GRCGYC 1 cut(s) 348
BssSI CACGAG 2 cut(s) 182, 303
BssT1I CCWWGG 2 cut(s) 86, 96
Bst2BI CACGAG 2 cut(s) 182, 303
Bst2UI CCWGG 1 cut(s) 255
Bst6I CTCTTC 1 cut(s) 57
BstACI GRCGYC 1 cut(s) 348
BstBI TTCGAA 1 cut(s) 143
BstDEI CTNAG 1 cut(s) 260
BstMAI GTCTC 4 cut(s) 225, 302, 431, 449
BstMWI GCNNNNNNNGC 2 cut(s) 169, 340
BstNI CCWGG 1 cut(s) 255
BstNSI RCATGY 1 cut(s) 43
BstSCI CCNGG 1 cut(s) 253
BstSLI GKGCMC 2 cut(s) 279, 366
BstV1I GCAGC 1 cut(s) 139
BstV2I GAAGAC 2 cut(s) 210, 388
BtsIMutI CAGTG 1 cut(s) 471
BveI ACCTGC 1 cut(s) 34
CciI TCATGA 1 cut(s) 447
Cfr13I GGNCC 1 cut(s) 242
CseI GACGC 1 cut(s) 356
CviAII CATG 3 cut(s) 40, 367, 448
CviJI RGCY 9 cut(s) 59, 81, 101, 134, 152, 343, 356, 456, 504
CviKI_1 RGCY 9 cut(s) 59, 81, 101, 134, 152, 343, 356, 456, 504
DdeI CTNAG 1 cut(s) 260
DraIII CACNNNGTG 1 cut(s) 463
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
Eco130I CCWWGG 2 cut(s) 86, 96
Eco47I GGWCC 1 cut(s) 242
Eco57I CTGAAG 2 cut(s) 81, 371
Eco88I CYCGRG 1 cut(s) 495
EcoO109I RGGNCCY 1 cut(s) 242
EcoRII CCWGG 1 cut(s) 253
EcoT14I CCWWGG 2 cut(s) 86, 96
ErhI CCWWGG 2 cut(s) 86, 96
FaeI CATG 3 cut(s) 43, 370, 451
FaiI YATR 5 cut(s) 20, 33, 41, 368, 449
FaqI GGGAC 2 cut(s) 172, 228
FatI CATG 3 cut(s) 39, 366, 447
Fnu4HI GCNGC 1 cut(s) 153
Fsp4HI GCNGC 1 cut(s) 153
FspBI CTAG 2 cut(s) 56, 441
GluI GCNGC 1 cut(s) 153
HgaI GACGC 1 cut(s) 356
Hin1I GRCGYC 1 cut(s) 348
Hin1II CATG 3 cut(s) 43, 370, 451
HinfI GANTC 4 cut(s) 185, 310, 323, 488
Hpy166II GTNNAC 1 cut(s) 364
Hpy188I TCNGA 3 cut(s) 76, 121, 223
Hpy188III TCNNGA 3 cut(s) 305, 389, 448
Hpy8I GTNNAC 1 cut(s) 364
Hpy99I CGWCG 1 cut(s) 334
HpyAV CCTTC 1 cut(s) 71
HpyCH4V TGCA 7 cut(s) 4, 43, 172, 194, 320, 364, 466
HpyF10VI GCNNNNNNNGC 2 cut(s) 169, 340
HpyF3I CTNAG 1 cut(s) 260
Hsp92I GRCGYC 1 cut(s) 348
Hsp92II CATG 3 cut(s) 43, 370, 451
KflI GGGWCCC 1 cut(s) 242
LpnPI CCDG 8 cut(s) 29, 213, 224, 240, 267, 348, 357, 374
Lsp1109I GCAGC 1 cut(s) 139
MaeI CTAG 2 cut(s) 56, 441
MaeIII GTNAC 1 cut(s) 110
MboII GAAGA 4 cut(s) 74, 210, 318, 393
MhlI GDGCHC 2 cut(s) 279, 366
MluCI AATT 3 cut(s) 146, 166, 198
MlyI GAGTC 3 cut(s) 194, 304, 482
MmeI TCCRAC 1 cut(s) 394
MnlI CCTC 4 cut(s) 258, 385, 491, 494
MspR9I CCNGG 1 cut(s) 255
MvaI CCWGG 1 cut(s) 255
MwoI GCNNNNNNNGC 2 cut(s) 169, 340
NlaIII CATG 3 cut(s) 43, 370, 451
NlaIV GGNNCC 2 cut(s) 243, 244
NmuCI GTSAC 1 cut(s) 110
NspI RCATGY 1 cut(s) 43
NspV TTCGAA 1 cut(s) 143
PagI TCATGA 1 cut(s) 447
PfeI GAWTC 1 cut(s) 323
PkrI GCNGC 1 cut(s) 154
PleI GAGTC 3 cut(s) 193, 304, 482
PpsI GAGTC 3 cut(s) 193, 304, 482
PpuMI RGGWCCY 1 cut(s) 242
Psp5II RGGWCCY 1 cut(s) 242
Psp6I CCWGG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 2 cut(s) 243, 244
PspPI GGNCC 1 cut(s) 242
PspPPI RGGWCCY 1 cut(s) 242
SatI GCNGC 1 cut(s) 153
Sau96I GGNCC 1 cut(s) 242
SchI GAGTC 3 cut(s) 194, 304, 482
ScrFI CCNGG 1 cut(s) 255
SduI GDGCHC 2 cut(s) 279, 366
SetI ASST 3 cut(s) 48, 61, 154
SfuI TTCGAA 1 cut(s) 143
SinI GGWCC 1 cut(s) 242
SpeI ACTAGT 1 cut(s) 440
Sse9I AATT 3 cut(s) 146, 166, 198
SspMI CTAG 2 cut(s) 56, 441
StyD4I CCNGG 1 cut(s) 253
StyI CCWWGG 2 cut(s) 86, 96
TaqI TCGA 2 cut(s) 129, 143
TasI AATT 3 cut(s) 146, 166, 198
TfiI GAWTC 1 cut(s) 323
TscAI CASTG 1 cut(s) 478
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 1 cut(s) 152
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 1 cut(s) 420
TspGWI ACGGA 1 cut(s) 451
TspRI CASTG 1 cut(s) 478
VneI GTGCAC 1 cut(s) 362
VpaK11BI GGWCC 1 cut(s) 242
XapI RAATTY 1 cut(s) 198
XceI RCATGY 1 cut(s) 43
XspI CTAG 2 cut(s) 56, 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.