Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0003166

Physical Location & Seq

Genomic Coordinates Reverse (-)

8858224 .. 8862303

Gene Structure 4080 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1104600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 873 bp

ATGTGGGATTTTGGGATGAGAATCAGTAAAACAGAGGTGAATTTGAAGAGGCTGCTTGCAGCTGCGCCTCAGCAACAGAACCAGGCAAAACTTGTTCATTATGTTGCTACTTTACGAGAACTGGTAGAACAACTAGCTGAAGAGAGAACTCCAGAAGGCTTACCCAGGTCAATTTTGGTAGGTGTGATGGAAATATTGATTCACATTCCAAACACTATTTTCTTGATACTGGGTACTAATGGTGGCTACACAGATCAACATTTTCATCGTCTCTATTTCTATGCTGTTTTGAGTGACTATTCAGAGAAGATTGAAGCCATTGCTTCCAAATTAGCTGCTCCACCACCTGATATTCAAACACCCGAAGTGCCCCTTGCAAGAATTTCTGTCAAAGCAAACTCTTCTGATACAGGAGACAATCAGATTCCCCCTTCTCCAGGTTTGAGAAGGAGATTTGTGCCTATCTCAAATACTGAAGATGGAACTCGCGAGACTGCTGGTGTTGATTCTTCAGGGCCTGTCAAACTGGACGCAGCAGCCCAAGCACACATTGAAAAGCATAGAAAGCTTCAAGAGGATTTGACAGACGAAATGGTTGGATTGGCTAGACAGCTCAAAGAGAGTAGTCTCATGATGAGTCACTCCCTGGAAAACACTGAAAAAGTAAGCACCCTGAGATATCTATTGCAGGATGTTATACTTGATTCTACAGAGAAGGCTGTTGAAAGTAGCTTGGCAAGCACCCGTCACGCCACTACACGGGCAGCAGGTATATACTCAAAGGCGTCCAAGACTACTTGTTTCACATGGCTTCTGATGTTTGTCATGACATTTGTATTTATCATGGTGGTTCTTTTAATTCGCGTAACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04130 map04130

Pfam Domains

Protein Families

Use1

Protein Analysis

291

Amino Acids

32.28

Weight (kDa)

6.46

Isoelectric Point (pI)

51.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Use1	PF09753	10 - 51	1.3e-09	Membrane fusion protein Use1
Use1	PF09753	95 - 290	2.8e-44	Membrane fusion protein Use1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003166)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54110 AT1G54110 AT1G54110 AT3G55600 AT3G55600
fragaria_vesca	FvH4_2g10880 FvH4_6g47540
malus_domestica	MD08G1104600.v1.1 MD13G1238800.v1.1
prunus_persica	Prupe.1G071200_v2.0.a1 Prupe.1G071200_v2.0.a1 Prupe.1G071200_v2.0.a1 Prupe.1G071200_v2.0.a1 Prupe.2G148000_v2.0.a1
pyrus_communis	pycom13g21130 pycom16g20480
rosa_chinensis	RchiOBHm_Chr5g0056791 RchiOBHm_Chr6g0268981
rosa_laevigata	RLG00000013891 RLG00000035127
rosa_multiflora	Rmu_sc0001460.1_g000050
rosa_roxburghii	Rroxscaffold_1G00023600 Rroxscaffold_2G00084380 Rroxscaffold_7G00199120
rosa_rugosa	Rorug05G0301900 Rorug06G0043600 Rorug06G0044000
rosa_samantha	Rh5AG372600 Rh5BG383900 Rh5CG407000 Rh5DG397000 Rh6AG164700 Rh6BG169900 Rh6CG163400 Rh6DG155200
rosa_wichuraiana	Rw5G034920 Rw6G014160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	758
AccII	CGCG	2 cut(s)	489, 864
AcsI	RAATTY	2 cut(s)	40, 381
AcuI	CTGAAG	3 cut(s)	159, 495, 495
AcyI	GRCGYC	1 cut(s)	785
AfaI	GTAC	1 cut(s)	235
AfiI	CCNNNNNNNGG	2 cut(s)	437, 759
AgsI	TTSAA	6 cut(s)	46, 314, 356, 554, 572, 725
AjnI	CCWGG	4 cut(s)	81, 164, 436, 645
AluBI	AGCT	6 cut(s)	62, 137, 335, 568, 613, 732
AluI	AGCT	6 cut(s)	62, 137, 335, 568, 613, 732
Alw26I	GTCTC	4 cut(s)	275, 408, 485, 632
AlwNI	CAGNNNCTG	1 cut(s)	518
AoxI	GGCC	1 cut(s)	515
ApeKI	GCWGC	7 cut(s)	52, 59, 62, 335, 533, 536, 764
ApoI	RAATTY	2 cut(s)	40, 381
AspLEI	GCGC	1 cut(s)	67
AspS9I	GGNCC	1 cut(s)	515
AsuHPI	GGTGA	1 cut(s)	49
BaeGI	GKGCMC	1 cut(s)	372
BbvCI	CCTCAGC	1 cut(s)	69
BbvI	GCAGC	7 cut(s)	39, 49, 71, 322, 545, 548, 776
BccI	CCATC	2 cut(s)	181, 473
BciT130I	CCWGG	4 cut(s)	83, 166, 438, 647
BcoDI	GTCTC	4 cut(s)	275, 408, 485, 632
BfaI	CTAG	2 cut(s)	134, 606
BfmI	CTRYAG	1 cut(s)	708
BfuAI	ACCTGC	1 cut(s)	758
BisI	GCNGC	7 cut(s)	53, 60, 63, 336, 534, 537, 765
BlsI	GCNGC	7 cut(s)	54, 61, 64, 337, 535, 538, 766
Bme1390I	CCNGG	4 cut(s)	83, 166, 438, 647
BmgT120I	GGNCC	1 cut(s)	515
BmrFI	CCNGG	4 cut(s)	83, 166, 438, 647
BmrI	ACTGGG	1 cut(s)	239
BmuI	ACTGGG	1 cut(s)	239
BpmI	CTGGAG	2 cut(s)	135, 420
Bpu10I	CCTNAGC	1 cut(s)	69
BsaBI	GATNNNNATC	1 cut(s)	20
BsaHI	GRCGYC	1 cut(s)	785
BsaJI	CCNNGG	2 cut(s)	164, 645
Bsc4I	CCNNNNNNNGG	2 cut(s)	437, 759
Bse1I	ACTGG	3 cut(s)	126, 234, 531
Bse3DI	GCAATG	1 cut(s)	318
Bse8I	GATNNNNATC	1 cut(s)	20
BseBI	CCWGG	4 cut(s)	83, 166, 438, 647
BseDI	CCNNGG	2 cut(s)	164, 645
BseGI	GGATG	2 cut(s)	21, 697
BseJI	GATNNNNATC	1 cut(s)	20
BseLI	CCNNNNNNNGG	2 cut(s)	437, 759
BseMI	GCAATG	1 cut(s)	318
BseMII	CTCAG	2 cut(s)	83, 665
BseNI	ACTGG	3 cut(s)	126, 234, 531
BseSI	GKGCMC	1 cut(s)	372
BseXI	GCAGC	7 cut(s)	39, 49, 71, 322, 545, 548, 776
Bsh1236I	CGCG	2 cut(s)	489, 864
BshFI	GGCC	1 cut(s)	517
BslI	CCNNNNNNNGG	2 cut(s)	437, 759
BsmAI	GTCTC	4 cut(s)	275, 408, 485, 632
BsmBI	CGTCTC	1 cut(s)	275
BsnI	GGCC	1 cut(s)	517
Bsp1286I	GDGCHC	1 cut(s)	372
Bsp143I	GATC	1 cut(s)	253
Bsp68I	TCGCGA	1 cut(s)	489
BspANI	GGCC	1 cut(s)	517
BspCNI	CTCAG	2 cut(s)	82, 666
BspFNI	CGCG	2 cut(s)	489, 864
BspHI	TCATGA	2 cut(s)	630, 825
BspMI	ACCTGC	1 cut(s)	758
BsrDI	GCAATG	1 cut(s)	318
BsrI	ACTGG	3 cut(s)	126, 234, 531
BssECI	CCNNGG	2 cut(s)	164, 645
BssMI	GATC	1 cut(s)	253
BssNI	GRCGYC	1 cut(s)	785
Bst2UI	CCWGG	4 cut(s)	83, 166, 438, 647
Bst6I	CTCTTC	3 cut(s)	41, 135, 406
BstACI	GRCGYC	1 cut(s)	785
BstC8I	GCNNGC	2 cut(s)	57, 739
BstDEI	CTNAG	2 cut(s)	69, 674
BstENI	CCTNNNNNAGG	1 cut(s)	435
BstF5I	GGATG	2 cut(s)	21, 697
BstFNI	CGCG	2 cut(s)	489, 864
BstHHI	GCGC	1 cut(s)	67
BstKTI	GATC	1 cut(s)	256
BstMAI	GTCTC	4 cut(s)	275, 408, 485, 632
BstMBI	GATC	1 cut(s)	253
BstMWI	GCNNNNNNNGC	3 cut(s)	542, 565, 738
BstNI	CCWGG	4 cut(s)	83, 166, 438, 647
BstSCI	CCNGG	4 cut(s)	81, 164, 436, 645
BstSFI	CTRYAG	1 cut(s)	708
BstSLI	GKGCMC	1 cut(s)	372
BstUI	CGCG	2 cut(s)	489, 864
BstV1I	GCAGC	7 cut(s)	39, 49, 71, 322, 545, 548, 776
BsuRI	GGCC	1 cut(s)	517
BtsCI	GGATG	2 cut(s)	21, 697
BtsIMutI	CAGTG	1 cut(s)	654
BtuMI	TCGCGA	1 cut(s)	489
BveI	ACCTGC	1 cut(s)	758
Cac8I	GCNNGC	2 cut(s)	57, 739
CaiI	CAGNNNCTG	1 cut(s)	518
CciI	TCATGA	2 cut(s)	630, 825
CfoI	GCGC	1 cut(s)	67
Cfr13I	GGNCC	1 cut(s)	515
CseI	GACGC	2 cut(s)	539, 774
Csp6I	GTAC	1 cut(s)	234
CviAII	CATG	4 cut(s)	631, 807, 826, 844
CviQI	GTAC	1 cut(s)	234
DdeI	CTNAG	2 cut(s)	69, 674
DpnI	GATC	1 cut(s)	255
DpnII	GATC	1 cut(s)	253
Eam1104I	CTCTTC	3 cut(s)	41, 135, 406
EarI	CTCTTC	3 cut(s)	41, 135, 406
Eco32I	GATATC	1 cut(s)	680
Eco57I	CTGAAG	3 cut(s)	159, 495, 495
EcoNI	CCTNNNNNAGG	1 cut(s)	435
EcoO109I	RGGNCCY	1 cut(s)	515
EcoRII	CCWGG	4 cut(s)	81, 164, 436, 645
EcoRV	GATATC	1 cut(s)	680
Esp3I	CGTCTC	1 cut(s)	275
FaeI	CATG	4 cut(s)	634, 810, 829, 847
FalI	AAGNNNNNCTT	2 cut(s)	357, 389
FatI	CATG	4 cut(s)	630, 806, 825, 843
Fnu4HI	GCNGC	7 cut(s)	53, 60, 63, 336, 534, 537, 765
FokI	GGATG	2 cut(s)	28, 704
Fsp4HI	GCNGC	7 cut(s)	53, 60, 63, 336, 534, 537, 765
FspBI	CTAG	2 cut(s)	134, 606
GlaI	GCGC	1 cut(s)	66
GluI	GCNGC	7 cut(s)	53, 60, 63, 336, 534, 537, 765
GsuI	CTGGAG	2 cut(s)	135, 420
HaeIII	GGCC	1 cut(s)	517
HgaI	GACGC	2 cut(s)	539, 774
HhaI	GCGC	1 cut(s)	67
Hin1I	GRCGYC	1 cut(s)	785
Hin1II	CATG	4 cut(s)	634, 810, 829, 847
Hin6I	GCGC	1 cut(s)	65
HinP1I	GCGC	1 cut(s)	65
HindIII	AAGCTT	1 cut(s)	566
HinfI	GANTC	6 cut(s)	21, 199, 424, 506, 637, 704
HphI	GGTGA	1 cut(s)	49
Hpy188I	TCNGA	4 cut(s)	304, 406, 423, 816
Hpy188III	TCNNGA	6 cut(s)	152, 223, 488, 572, 631, 826
HpyAV	CCTTC	4 cut(s)	149, 441, 441, 709
HpyCH4V	TGCA	3 cut(s)	59, 377, 688
HpyF10VI	GCNNNNNNNGC	3 cut(s)	542, 565, 738
HpyF3I	CTNAG	2 cut(s)	69, 674
Hsp92I	GRCGYC	1 cut(s)	785
Hsp92II	CATG	4 cut(s)	634, 810, 829, 847
HspAI	GCGC	1 cut(s)	65
Kzo9I	GATC	1 cut(s)	253
LmnI	GCTCC	1 cut(s)	343
Lsp1109I	GCAGC	7 cut(s)	39, 49, 71, 322, 545, 548, 776
MaeI	CTAG	2 cut(s)	134, 606
MaeIII	GTNAC	4 cut(s)	293, 638, 746, 865
MalI	GATC	1 cut(s)	255
MboI	GATC	1 cut(s)	253
MboII	GAAGA	6 cut(s)	58, 152, 319, 393, 488, 501
MhlI	GDGCHC	1 cut(s)	372
MluCI	AATT	5 cut(s)	40, 171, 329, 381, 858
MlyI	GAGTC	1 cut(s)	646
MmeI	TCCRAC	1 cut(s)	577
MnlI	CCTC	4 cut(s)	28, 42, 78, 568
MseI	TTAA	2 cut(s)	857, 871
MspA1I	CMGCKG	1 cut(s)	62
MspR9I	CCNGG	4 cut(s)	83, 166, 438, 647
MvaI	CCWGG	4 cut(s)	83, 166, 438, 647
MvnI	CGCG	2 cut(s)	489, 864
MwoI	GCNNNNNNNGC	3 cut(s)	542, 565, 738
NdeII	GATC	1 cut(s)	253
NlaIII	CATG	4 cut(s)	634, 810, 829, 847
NmuCI	GTSAC	3 cut(s)	293, 638, 746
NruI	TCGCGA	1 cut(s)	489
PagI	TCATGA	2 cut(s)	630, 825
PfeI	GAWTC	5 cut(s)	21, 199, 424, 506, 704
PkrI	GCNGC	7 cut(s)	54, 61, 64, 337, 535, 538, 766
PleI	GAGTC	1 cut(s)	645
PpsI	GAGTC	1 cut(s)	645
Psp6I	CCWGG	4 cut(s)	81, 164, 436, 645
PspGI	CCWGG	4 cut(s)	81, 164, 436, 645
PspPI	GGNCC	1 cut(s)	515
PstNI	CAGNNNCTG	1 cut(s)	518
PvuII	CAGCTG	1 cut(s)	62
RruI	TCGCGA	1 cut(s)	489
RsaI	GTAC	1 cut(s)	235
RsaNI	GTAC	1 cut(s)	234
SaqAI	TTAA	2 cut(s)	857, 871
SatI	GCNGC	7 cut(s)	53, 60, 63, 336, 534, 537, 765
Sau3AI	GATC	1 cut(s)	253
Sau96I	GGNCC	1 cut(s)	515
SchI	GAGTC	1 cut(s)	646
ScrFI	CCNGG	4 cut(s)	83, 166, 438, 647
SduI	GDGCHC	1 cut(s)	372
SfcI	CTRYAG	1 cut(s)	708
Sse9I	AATT	5 cut(s)	40, 171, 329, 381, 858
SspI	AATATT	1 cut(s)	195
SspMI	CTAG	2 cut(s)	134, 606
StyD4I	CCNGG	4 cut(s)	81, 164, 436, 645
TasI	AATT	5 cut(s)	40, 171, 329, 381, 858
TfiI	GAWTC	5 cut(s)	21, 199, 424, 506, 704
Tru1I	TTAA	2 cut(s)	857, 871
Tru9I	TTAA	2 cut(s)	857, 871
TscAI	CASTG	1 cut(s)	661
TseFI	GTSAC	3 cut(s)	293, 638, 746
TseI	GCWGC	7 cut(s)	52, 59, 62, 335, 533, 536, 764
Tsp45I	GTSAC	3 cut(s)	293, 638, 746
TspDTI	ATGAA	2 cut(s)	86, 254
TspRI	CASTG	1 cut(s)	661
XagI	CCTNNNNNAGG	1 cut(s)	435
XapI	RAATTY	2 cut(s)	40, 381
XcmI	CCANNNNNNNNNTGG	1 cut(s)	172
XspI	CTAG	2 cut(s)	134, 606

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.