MD13G1238800.v1.1

Vesicle transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003166
Physical Location & Seq
Forward (+)
24286994 .. 24291574
4581 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1238800.v1.1.491

Sequence Viewer

Length: 735 bp
ATGTTTGATTTTGGGATGAGAATCAGTAAAACAGAGGTGAATTTGAAGAGGCTGCTTGCAGCTGCGCCTCAGCAACAGAACCAGGCAAAACTTGTACATTATGTTGCTACTTTACGAGAACTGGTAGAACAACTAGCTGAAGAGAGAACTCCAGAAGGCTTACCAAGAGTTTCAAAGGCTGTTTTGAGTGACTATTCAGAGAAGATTGAAGCCATTGCTTCCAAATTAGCTGCTCCACCGCCTGATATTCAAACACCCGAAGAGCCCCTTGCAAGAATTTCTGTCAAAGCAAACTCTTCTGATACAGGAGACAATCAGATTCCCCCTTCTCCAGGTTTGAGAAGGAGATTTGTGCCAATCTCAAGTACTGAAGATGGAACTCGTGAGACTGCTGGTGTTGATTCTTCAGGGCCTGTCAAACTGGATGCTGCAGCCCAAGAACACATTGAAAAGCATAGAAAGCTTCAAGAGGATTTGACGGACGAAATGGTTGGATTGGCTAGGCAGCTCAAAGAGAGTAGTCTCATGATGAGTCACTCCCTGGAAAACACTGAAAAAATACTTGATTCTACAGAGAAGGCTGTTGAAAGTAGCTTGGCAAGCACTGGTCACGCCACTACACGGGCAGCAGGTATATACTCGAAGACATCCAAGACTACTTGTTTCACATGGCTTCTGATGTTTGTCATGACATGTGTATTTATCATGGTGGTTCTTTTAATTCGCGTCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 map04130
Pfam Domains
Protein Families
Use1

Protein Analysis

245

Amino Acids

26.82

Weight (kDa)

6.01

Isoelectric Point (pI)

51.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Use1 PF09753 10 - 244 1.6e-68 Membrane fusion protein Use1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 620
AccII CGCG 1 cut(s) 726
AciI CCGC 1 cut(s) 239
AcsI RAATTY 2 cut(s) 40, 276
AcuI CTGAAG 3 cut(s) 159, 390, 390
AfaI GTAC 2 cut(s) 96, 367
AfiI CCNNNNNNNGG 2 cut(s) 332, 621
AflIII ACRYGT 1 cut(s) 692
AgsI TTSAA 7 cut(s) 46, 174, 209, 251, 449, 467, 587
AjnI CCWGG 3 cut(s) 81, 331, 540
AluBI AGCT 6 cut(s) 62, 137, 230, 463, 508, 594
AluI AGCT 6 cut(s) 62, 137, 230, 463, 508, 594
Alw26I GTCTC 3 cut(s) 303, 380, 527
AlwNI CAGNNNCTG 1 cut(s) 413
AoxI GGCC 1 cut(s) 410
ApeKI GCWGC 8 cut(s) 52, 59, 62, 230, 428, 431, 505, 626
ApoI RAATTY 2 cut(s) 40, 276
AspLEI GCGC 1 cut(s) 67
AspS9I GGNCC 1 cut(s) 410
AsuHPI GGTGA 1 cut(s) 49
BanII GRGCYC 1 cut(s) 267
BauI CACGAG 1 cut(s) 381
BbsI GAAGAC 1 cut(s) 650
BbvCI CCTCAGC 1 cut(s) 69
BbvI GCAGC 8 cut(s) 39, 49, 71, 217, 415, 443, 517, 638
BccI CCATC 1 cut(s) 368
BciT130I CCWGG 3 cut(s) 83, 333, 542
BcoDI GTCTC 3 cut(s) 303, 380, 527
BfaI CTAG 2 cut(s) 134, 501
BfmI CTRYAG 2 cut(s) 429, 570
BfuAI ACCTGC 1 cut(s) 620
BisI GCNGC 8 cut(s) 53, 60, 63, 231, 429, 432, 506, 627
BlsI GCNGC 8 cut(s) 54, 61, 64, 232, 430, 433, 507, 628
BmcAI AGTACT 1 cut(s) 367
Bme1390I CCNGG 3 cut(s) 83, 333, 542
BmgT120I GGNCC 1 cut(s) 410
BmrFI CCNGG 3 cut(s) 83, 333, 542
BmsI GCATC 1 cut(s) 415
BpiI GAAGAC 1 cut(s) 650
BpmI CTGGAG 2 cut(s) 135, 315
Bpu10I CCTNAGC 1 cut(s) 69
BpuEI CTTGAG 1 cut(s) 346
BsaBI GATNNNNATC 1 cut(s) 20
BsaJI CCNNGG 1 cut(s) 540
Bsc4I CCNNNNNNNGG 2 cut(s) 332, 621
Bse1I ACTGG 3 cut(s) 126, 426, 610
Bse3DI GCAATG 1 cut(s) 213
Bse8I GATNNNNATC 1 cut(s) 20
BseBI CCWGG 3 cut(s) 83, 333, 542
BseDI CCNNGG 1 cut(s) 540
BseGI GGATG 3 cut(s) 21, 430, 647
BseJI GATNNNNATC 1 cut(s) 20
BseLI CCNNNNNNNGG 2 cut(s) 332, 621
BseMI GCAATG 1 cut(s) 213
BseMII CTCAG 1 cut(s) 83
BseNI ACTGG 3 cut(s) 126, 426, 610
BseXI GCAGC 8 cut(s) 39, 49, 71, 217, 415, 443, 517, 638
Bsh1236I CGCG 1 cut(s) 726
BshFI GGCC 1 cut(s) 412
BslI CCNNNNNNNGG 2 cut(s) 332, 621
BsmAI GTCTC 3 cut(s) 303, 380, 527
BsnI GGCC 1 cut(s) 412
Bsp1286I GDGCHC 1 cut(s) 267
Bsp1407I TGTACA 1 cut(s) 94
BspACI CCGC 1 cut(s) 239
BspANI GGCC 1 cut(s) 412
BspCNI CTCAG 1 cut(s) 82
BspFNI CGCG 1 cut(s) 726
BspHI TCATGA 2 cut(s) 525, 687
BspMAI CTGCAG 1 cut(s) 433
BspMI ACCTGC 1 cut(s) 620
BspQI GCTCTTC 1 cut(s) 255
BsrDI GCAATG 1 cut(s) 213
BsrGI TGTACA 1 cut(s) 94
BsrI ACTGG 3 cut(s) 126, 426, 610
BssECI CCNNGG 1 cut(s) 540
BssSI CACGAG 1 cut(s) 381
Bst2BI CACGAG 1 cut(s) 381
Bst2UI CCWGG 3 cut(s) 83, 333, 542
Bst6I CTCTTC 4 cut(s) 41, 135, 255, 301
BstAUI TGTACA 1 cut(s) 94
BstC8I GCNNGC 2 cut(s) 57, 601
BstDEI CTNAG 1 cut(s) 69
BstENI CCTNNNNNAGG 1 cut(s) 330
BstF5I GGATG 3 cut(s) 21, 430, 647
BstFNI CGCG 1 cut(s) 726
BstHHI GCGC 1 cut(s) 67
BstMAI GTCTC 3 cut(s) 303, 380, 527
BstMWI GCNNNNNNNGC 2 cut(s) 460, 600
BstNI CCWGG 3 cut(s) 83, 333, 542
BstNSI RCATGY 1 cut(s) 696
BstSCI CCNGG 3 cut(s) 81, 331, 540
BstSFI CTRYAG 2 cut(s) 429, 570
BstUI CGCG 1 cut(s) 726
BstV1I GCAGC 8 cut(s) 39, 49, 71, 217, 415, 443, 517, 638
BstV2I GAAGAC 1 cut(s) 650
BsuRI GGCC 1 cut(s) 412
BtsCI GGATG 3 cut(s) 21, 430, 647
BtsIMutI CAGTG 2 cut(s) 549, 603
BveI ACCTGC 1 cut(s) 620
Cac8I GCNNGC 2 cut(s) 57, 601
CaiI CAGNNNCTG 1 cut(s) 413
CciI TCATGA 2 cut(s) 525, 687
CfoI GCGC 1 cut(s) 67
Cfr13I GGNCC 1 cut(s) 410
CseI GACGC 1 cut(s) 715
Csp6I GTAC 2 cut(s) 95, 366
CviAII CATG 5 cut(s) 526, 669, 688, 693, 706
CviQI GTAC 2 cut(s) 95, 366
DdeI CTNAG 1 cut(s) 69
Eam1104I CTCTTC 4 cut(s) 41, 135, 255, 301
EarI CTCTTC 4 cut(s) 41, 135, 255, 301
Eco24I GRGCYC 1 cut(s) 267
Eco57I CTGAAG 3 cut(s) 159, 390, 390
EcoNI CCTNNNNNAGG 1 cut(s) 330
EcoO109I RGGNCCY 1 cut(s) 410
EcoRII CCWGG 3 cut(s) 81, 331, 540
EcoT38I GRGCYC 1 cut(s) 267
FaeI CATG 5 cut(s) 529, 672, 691, 696, 709
FaiI YATR 9 cut(s) 102, 456, 527, 635, 637, 670, 689, 694, 707
FalI AAGNNNNNCTT 2 cut(s) 252, 284
FatI CATG 5 cut(s) 525, 668, 687, 692, 705
Fnu4HI GCNGC 8 cut(s) 53, 60, 63, 231, 429, 432, 506, 627
FokI GGATG 3 cut(s) 28, 437, 634
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 8 cut(s) 53, 60, 63, 231, 429, 432, 506, 627
FspBI CTAG 2 cut(s) 134, 501
GlaI GCGC 1 cut(s) 66
GluI GCNGC 8 cut(s) 53, 60, 63, 231, 429, 432, 506, 627
GsuI CTGGAG 2 cut(s) 135, 315
HaeIII GGCC 1 cut(s) 412
HgaI GACGC 1 cut(s) 715
HhaI GCGC 1 cut(s) 67
Hin1II CATG 5 cut(s) 529, 672, 691, 696, 709
Hin6I GCGC 1 cut(s) 65
HinP1I GCGC 1 cut(s) 65
HindIII AAGCTT 1 cut(s) 461
HinfI GANTC 5 cut(s) 21, 319, 401, 532, 566
HphI GGTGA 1 cut(s) 49
Hpy188I TCNGA 4 cut(s) 199, 301, 318, 678
Hpy188III TCNNGA 5 cut(s) 152, 383, 467, 526, 688
HpyAV CCTTC 4 cut(s) 149, 336, 336, 571
HpyCH4V TGCA 3 cut(s) 59, 272, 431
HpyF10VI GCNNNNNNNGC 2 cut(s) 460, 600
HpyF3I CTNAG 1 cut(s) 69
Hsp92II CATG 5 cut(s) 529, 672, 691, 696, 709
HspAI GCGC 1 cut(s) 65
LguI GCTCTTC 1 cut(s) 255
LmnI GCTCC 1 cut(s) 238
Lsp1109I GCAGC 8 cut(s) 39, 49, 71, 217, 415, 443, 517, 638
LweI GCATC 1 cut(s) 415
MaeI CTAG 2 cut(s) 134, 501
MaeIII GTNAC 4 cut(s) 188, 533, 608, 727
MboII GAAGA 8 cut(s) 58, 152, 214, 272, 288, 383, 396, 655
MhlI GDGCHC 1 cut(s) 267
MluCI AATT 4 cut(s) 40, 224, 276, 720
MlyI GAGTC 1 cut(s) 541
MmeI TCCRAC 1 cut(s) 472
MnlI CCTC 4 cut(s) 28, 42, 78, 463
MseI TTAA 2 cut(s) 719, 733
MspA1I CMGCKG 1 cut(s) 62
MspR9I CCNGG 3 cut(s) 83, 333, 542
MvaI CCWGG 3 cut(s) 83, 333, 542
MvnI CGCG 1 cut(s) 726
MwoI GCNNNNNNNGC 2 cut(s) 460, 600
NlaIII CATG 5 cut(s) 529, 672, 691, 696, 709
NmuCI GTSAC 4 cut(s) 188, 533, 608, 727
NspI RCATGY 1 cut(s) 696
PagI TCATGA 2 cut(s) 525, 687
PciI ACATGT 1 cut(s) 692
PciSI GCTCTTC 1 cut(s) 255
PfeI GAWTC 4 cut(s) 21, 319, 401, 566
PkrI GCNGC 8 cut(s) 54, 61, 64, 232, 430, 433, 507, 628
PleI GAGTC 1 cut(s) 540
PpsI GAGTC 1 cut(s) 540
PscI ACATGT 1 cut(s) 692
Psp6I CCWGG 3 cut(s) 81, 331, 540
PspGI CCWGG 3 cut(s) 81, 331, 540
PspPI GGNCC 1 cut(s) 410
PstI CTGCAG 1 cut(s) 433
PstNI CAGNNNCTG 1 cut(s) 413
PvuII CAGCTG 1 cut(s) 62
RsaI GTAC 2 cut(s) 96, 367
RsaNI GTAC 2 cut(s) 95, 366
SapI GCTCTTC 1 cut(s) 255
SaqAI TTAA 2 cut(s) 719, 733
SatI GCNGC 8 cut(s) 53, 60, 63, 231, 429, 432, 506, 627
Sau96I GGNCC 1 cut(s) 410
ScaI AGTACT 1 cut(s) 367
SchI GAGTC 1 cut(s) 541
ScrFI CCNGG 3 cut(s) 83, 333, 542
SduI GDGCHC 1 cut(s) 267
SetI ASST 9 cut(s) 39, 64, 139, 232, 337, 465, 510, 596, 634
SfaNI GCATC 1 cut(s) 415
SfcI CTRYAG 2 cut(s) 429, 570
SmlI CTYRAG 1 cut(s) 361
SmoI CTYRAG 1 cut(s) 361
Sse9I AATT 4 cut(s) 40, 224, 276, 720
SsiI CCGC 1 cut(s) 239
SspMI CTAG 2 cut(s) 134, 501
StyD4I CCNGG 3 cut(s) 81, 331, 540
TaqI TCGA 1 cut(s) 641
TasI AATT 4 cut(s) 40, 224, 276, 720
TatI WGTACW 2 cut(s) 94, 365
TfiI GAWTC 4 cut(s) 21, 319, 401, 566
Tru1I TTAA 2 cut(s) 719, 733
Tru9I TTAA 2 cut(s) 719, 733
TscAI CASTG 2 cut(s) 556, 610
TseFI GTSAC 4 cut(s) 188, 533, 608, 727
TseI GCWGC 8 cut(s) 52, 59, 62, 230, 428, 431, 505, 626
Tsp45I GTSAC 4 cut(s) 188, 533, 608, 727
TspGWI ACGGA 1 cut(s) 494
TspRI CASTG 2 cut(s) 556, 610
XagI CCTNNNNNAGG 1 cut(s) 330
XapI RAATTY 2 cut(s) 40, 276
XceI RCATGY 1 cut(s) 696
XspI CTAG 2 cut(s) 134, 501
ZrmI AGTACT 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.