FvH4_6g47540

Vesicle transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003166
Physical Location & Seq
Reverse (-)
35990993 .. 35992330
1338 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g47540.t1

Sequence Viewer

Length: 714 bp
ATGAAAATCAGTAAAACAGAAGTGAACTTGAGGAGGCTGCTTGCAGCTGCTCCTCAGCAACAAAACCAGGCAAAGCTTTCACATTATGTTGCTACTTTACGAGAACAGGTAGAACAGCTAGCTGAAGAGAGAACGCCGGAAGGATTACCGAGAGTTTCCAAGGCTGTTTTGAGTGACTACTCTGAGAAGATCGAAGCCGTTGCTTCGAAATTAACTTCTGCATTGCCTGATTTGCGAGAACCGCAGGAGTCACTCGGAAGAATTTCGGTTAAAGCAGAGTCTTCTGAGACAGGAGATAGTCGGAGCCCTCCCGGTCTTAGAAGGAGATTTGTCCCCAGCTCGAATGTACAAGATGGAACTCGTGAGACGCTCAGTGCAGATTCTTTGTTGCCTGTCAAACTTGACGCTGCAGCGCAAGCGCATATTGAGAAGCATAGAAGACTTGAAGAGGATTTGACTGATGAAATGGTTGTGTTGGCAAGGCAACTCAAAGAGACTAGTCTTGTGATGAGCCACTCCTTGAAGAACGCTGAAAAACTACTTGACTCTACTGAGGAGGCTGTTGAAAAGAGCTTGGCAAGCACTGGTCATGTCACTGCACGCGCATCTGATATATACTCAAAGACCTCCAAGACTTCATGTGTCACATGGCTTTTGATGTTTCTAATGACAGGTATATTCATCATGGTGGCACTTTTAATTCGTGTAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 map04130
Pfam Domains
Protein Families
Use1

Protein Analysis

238

Amino Acids

26.19

Weight (kDa)

6.99

Isoelectric Point (pI)

58.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Use1 PF09753 5 - 237 9.6e-60 Membrane fusion protein Use1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 603
AciI CCGC 1 cut(s) 242
AcsI RAATTY 1 cut(s) 261
AcuI CTGAAG 1 cut(s) 144
AfaI GTAC 1 cut(s) 348
AgsI TTSAA 3 cut(s) 446, 523, 566
AhlI ACTAGT 1 cut(s) 497
AjnI CCWGG 1 cut(s) 66
AluBI AGCT 6 cut(s) 47, 76, 118, 122, 339, 573
AluI AGCT 6 cut(s) 47, 76, 118, 122, 339, 573
Alw26I GTCTC 3 cut(s) 281, 359, 488
ApeKI GCWGC 5 cut(s) 37, 44, 47, 407, 410
ApoI RAATTY 1 cut(s) 261
Asp700I GAANNNNTTC 1 cut(s) 262
AspLEI GCGC 3 cut(s) 415, 421, 605
AsuC2I CCSGG 1 cut(s) 312
AsuII TTCGAA 1 cut(s) 206
AsuNHI GCTAGC 1 cut(s) 118
BanII GRGCYC 1 cut(s) 308
BauI CACGAG 1 cut(s) 360
BbsI GAAGAC 2 cut(s) 273, 445
BbvCI CCTCAGC 1 cut(s) 54
BbvI GCAGC 5 cut(s) 24, 34, 56, 394, 422
BccI CCATC 1 cut(s) 347
BceAI ACGGC 1 cut(s) 182
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
BciT130I CCWGG 1 cut(s) 68
BcnI CCSGG 1 cut(s) 312
BcoDI GTCTC 3 cut(s) 281, 359, 488
BcuI ACTAGT 1 cut(s) 497
BfaI CTAG 2 cut(s) 119, 498
BfmI CTRYAG 1 cut(s) 408
BisI GCNGC 5 cut(s) 38, 45, 48, 408, 411
BlsI GCNGC 5 cut(s) 39, 46, 49, 409, 412
Bme1390I CCNGG 2 cut(s) 68, 312
BmiI GGNNCC 1 cut(s) 305
BmrFI CCNGG 2 cut(s) 68, 312
BmsI GCATC 1 cut(s) 614
BmtI GCTAGC 1 cut(s) 122
BpiI GAAGAC 2 cut(s) 273, 445
Bpu10I CCTNAGC 1 cut(s) 54
Bpu14I TTCGAA 1 cut(s) 206
BpuEI CTTGAG 1 cut(s) 49
BpuMI CCSGG 1 cut(s) 312
BsaJI CCNNGG 1 cut(s) 159
Bse1I ACTGG 1 cut(s) 589
Bse3DI GCAATG 1 cut(s) 221
BseBI CCWGG 1 cut(s) 68
BseDI CCNNGG 1 cut(s) 159
BseMI GCAATG 1 cut(s) 221
BseMII CTCAG 5 cut(s) 68, 174, 276, 385, 543
BseNI ACTGG 1 cut(s) 589
BseRI GAGGAG 3 cut(s) 42, 46, 569
BseXI GCAGC 5 cut(s) 24, 34, 56, 394, 422
BseYI CCCAGC 1 cut(s) 335
BsgI GTGCAG 2 cut(s) 396, 582
Bsh1236I CGCG 1 cut(s) 603
BsiSI CCGG 2 cut(s) 137, 312
BslFI GGGAC 1 cut(s) 317
BsmAI GTCTC 3 cut(s) 281, 359, 488
BsmBI CGTCTC 1 cut(s) 359
BsmFI GGGAC 1 cut(s) 317
Bsp119I TTCGAA 1 cut(s) 206
Bsp1286I GDGCHC 1 cut(s) 308
Bsp1407I TGTACA 1 cut(s) 346
Bsp143I GATC 1 cut(s) 189
BspACI CCGC 1 cut(s) 242
BspCNI CTCAG 5 cut(s) 67, 175, 277, 384, 544
BspFNI CGCG 1 cut(s) 603
BspLI GGNNCC 1 cut(s) 305
BspMAI CTGCAG 1 cut(s) 412
BspOI GCTAGC 1 cut(s) 122
BspT104I TTCGAA 1 cut(s) 206
BsrDI GCAATG 1 cut(s) 221
BsrGI TGTACA 1 cut(s) 346
BsrI ACTGG 1 cut(s) 589
BssECI CCNNGG 1 cut(s) 159
BssMI GATC 1 cut(s) 189
BssSI CACGAG 1 cut(s) 360
BssT1I CCWWGG 1 cut(s) 159
Bst2BI CACGAG 1 cut(s) 360
Bst2UI CCWGG 1 cut(s) 68
Bst6I CTCTTC 2 cut(s) 120, 441
BstAUI TGTACA 1 cut(s) 346
BstBI TTCGAA 1 cut(s) 206
BstC8I GCNNGC 5 cut(s) 42, 120, 417, 580, 601
BstDEI CTNAG 6 cut(s) 54, 183, 285, 317, 371, 552
BstFNI CGCG 1 cut(s) 603
BstHHI GCGC 3 cut(s) 415, 421, 605
BstKTI GATC 1 cut(s) 192
BstMAI GTCTC 3 cut(s) 281, 359, 488
BstMBI GATC 1 cut(s) 189
BstMWI GCNNNNNNNGC 4 cut(s) 232, 241, 416, 579
BstNI CCWGG 1 cut(s) 68
BstSCI CCNGG 2 cut(s) 66, 310
BstSFI CTRYAG 1 cut(s) 408
BstUI CGCG 1 cut(s) 603
BstV1I GCAGC 5 cut(s) 24, 34, 56, 394, 422
BstV2I GAAGAC 2 cut(s) 273, 445
BtsI GCAGTG 1 cut(s) 594
BtsIMutI CAGTG 3 cut(s) 379, 582, 594
Cac8I GCNNGC 5 cut(s) 42, 120, 417, 580, 601
CfoI GCGC 3 cut(s) 415, 421, 605
CseI GACGC 2 cut(s) 376, 413
Csp6I GTAC 1 cut(s) 347
CviAII CATG 4 cut(s) 590, 639, 648, 685
CviQI GTAC 1 cut(s) 347
DdeI CTNAG 6 cut(s) 54, 183, 285, 317, 371, 552
DpnI GATC 1 cut(s) 191
DpnII GATC 1 cut(s) 189
Eam1104I CTCTTC 2 cut(s) 120, 441
EarI CTCTTC 2 cut(s) 120, 441
Eco130I CCWWGG 1 cut(s) 159
Eco24I GRGCYC 1 cut(s) 308
Eco57I CTGAAG 1 cut(s) 144
EcoRII CCWGG 1 cut(s) 66
EcoT14I CCWWGG 1 cut(s) 159
EcoT38I GRGCYC 1 cut(s) 308
ErhI CCWWGG 1 cut(s) 159
Esp3I CGTCTC 1 cut(s) 359
FaeI CATG 4 cut(s) 593, 642, 651, 688
FaqI GGGAC 1 cut(s) 317
FatI CATG 4 cut(s) 589, 638, 647, 684
Fnu4HI GCNGC 5 cut(s) 38, 45, 48, 408, 411
FriOI GRGCYC 1 cut(s) 308
Fsp4HI GCNGC 5 cut(s) 38, 45, 48, 408, 411
FspBI CTAG 2 cut(s) 119, 498
GlaI GCGC 3 cut(s) 414, 420, 604
GluI GCNGC 5 cut(s) 38, 45, 48, 408, 411
GsaI CCCAGC 1 cut(s) 339
HapII CCGG 2 cut(s) 137, 312
HgaI GACGC 2 cut(s) 376, 413
HhaI GCGC 3 cut(s) 415, 421, 605
Hin1II CATG 4 cut(s) 593, 642, 651, 688
Hin6I GCGC 3 cut(s) 413, 419, 603
HinP1I GCGC 3 cut(s) 413, 419, 603
HindIII AAGCTT 1 cut(s) 74
HinfI GANTC 4 cut(s) 248, 278, 380, 545
HpaII CCGG 2 cut(s) 137, 312
Hpy166II GTNNAC 1 cut(s) 25
Hpy188I TCNGA 5 cut(s) 184, 257, 286, 303, 610
Hpy188III TCNNGA 1 cut(s) 362
Hpy8I GTNNAC 1 cut(s) 25
HpyAV CCTTC 2 cut(s) 134, 315
HpyCH4V TGCA 5 cut(s) 44, 221, 377, 410, 599
HpyF10VI GCNNNNNNNGC 4 cut(s) 232, 241, 416, 579
HpyF3I CTNAG 6 cut(s) 54, 183, 285, 317, 371, 552
Hsp92II CATG 4 cut(s) 593, 642, 651, 688
HspAI GCGC 3 cut(s) 413, 419, 603
Kzo9I GATC 1 cut(s) 189
LmnI GCTCC 2 cut(s) 55, 303
Lsp1109I GCAGC 5 cut(s) 24, 34, 56, 394, 422
LweI GCATC 1 cut(s) 614
MaeI CTAG 2 cut(s) 119, 498
MaeIII GTNAC 5 cut(s) 173, 249, 592, 643, 706
MalI GATC 1 cut(s) 191
MboI GATC 1 cut(s) 189
MboII GAAGA 7 cut(s) 137, 199, 270, 273, 450, 458, 535
MhlI GDGCHC 1 cut(s) 308
MluCI AATT 3 cut(s) 209, 261, 699
MlyI GAGTC 3 cut(s) 257, 287, 539
MmeI TCCRAC 1 cut(s) 281
MnlI CCTC 8 cut(s) 24, 27, 63, 318, 442, 547, 550, 637
MroXI GAANNNNTTC 1 cut(s) 262
MseI TTAA 3 cut(s) 212, 270, 698
MslI CAYNNNNRTG 1 cut(s) 686
MspA1I CMGCKG 1 cut(s) 47
MspI CCGG 2 cut(s) 137, 312
MspR9I CCNGG 2 cut(s) 68, 312
MvaI CCWGG 1 cut(s) 68
MvnI CGCG 1 cut(s) 603
MwoI GCNNNNNNNGC 4 cut(s) 232, 241, 416, 579
NciI CCSGG 1 cut(s) 312
NdeII GATC 1 cut(s) 189
NheI GCTAGC 1 cut(s) 118
NlaIII CATG 4 cut(s) 593, 642, 651, 688
NlaIV GGNNCC 1 cut(s) 305
NmuCI GTSAC 4 cut(s) 173, 249, 592, 643
NspV TTCGAA 1 cut(s) 206
PdmI GAANNNNTTC 1 cut(s) 262
PfeI GAWTC 1 cut(s) 380
PkrI GCNGC 5 cut(s) 39, 46, 49, 409, 412
PleI GAGTC 3 cut(s) 256, 286, 539
PpsI GAGTC 3 cut(s) 256, 286, 539
Psp6I CCWGG 1 cut(s) 66
PspFI CCCAGC 1 cut(s) 335
PspGI CCWGG 1 cut(s) 66
PspN4I GGNNCC 1 cut(s) 305
PstI CTGCAG 1 cut(s) 412
PvuII CAGCTG 1 cut(s) 47
RsaI GTAC 1 cut(s) 348
RsaNI GTAC 1 cut(s) 347
RseI CAYNNNNRTG 1 cut(s) 686
SaqAI TTAA 3 cut(s) 212, 270, 698
SatI GCNGC 5 cut(s) 38, 45, 48, 408, 411
Sau3AI GATC 1 cut(s) 189
SchI GAGTC 3 cut(s) 257, 287, 539
ScrFI CCNGG 2 cut(s) 68, 312
SduI GDGCHC 1 cut(s) 308
SetI ASST 9 cut(s) 49, 78, 111, 120, 124, 341, 575, 629, 676
SfaNI GCATC 1 cut(s) 614
SfcI CTRYAG 1 cut(s) 408
SfuI TTCGAA 1 cut(s) 206
SmiMI CAYNNNNRTG 1 cut(s) 686
SmlI CTYRAG 1 cut(s) 28
SmoI CTYRAG 1 cut(s) 28
SpeI ACTAGT 1 cut(s) 497
Sse9I AATT 3 cut(s) 209, 261, 699
SsiI CCGC 1 cut(s) 242
SspMI CTAG 2 cut(s) 119, 498
StyD4I CCNGG 2 cut(s) 66, 310
StyI CCWWGG 1 cut(s) 159
TaqI TCGA 3 cut(s) 192, 206, 341
TasI AATT 3 cut(s) 209, 261, 699
TatI WGTACW 1 cut(s) 346
TfiI GAWTC 1 cut(s) 380
Tru1I TTAA 3 cut(s) 212, 270, 698
Tru9I TTAA 3 cut(s) 212, 270, 698
TscAI CASTG 3 cut(s) 379, 589, 601
TseFI GTSAC 4 cut(s) 173, 249, 592, 643
TseI GCWGC 5 cut(s) 37, 44, 47, 407, 410
Tsp45I GTSAC 4 cut(s) 173, 249, 592, 643
TspDTI ATGAA 4 cut(s) 17, 477, 627, 670
TspRI CASTG 3 cut(s) 379, 589, 601
XapI RAATTY 1 cut(s) 261
XmnI GAANNNNTTC 1 cut(s) 262
XspI CTAG 2 cut(s) 119, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.