Rw6G014160

Vesicle transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0003166
Physical Location & Seq
Forward (+)
26275346 .. 26276064
719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G014160.1

Sequence Viewer

Length: 636 bp
ATGAAAATCAGTAAAACAGAGGTGAACTTGAGGAGGCTGCTTGCAGCTGCCCTCAAACAGGTAGAACAGCTAGCTGAAGAGAGAACACCGGAAGGCTTACCTAGAGTTTCAAAGGCTGTCTTAAGTGACTATTCAGAGAAGATCGAAGCCATTGCTTCGAAATTAGCTGTTCCATTGCCTGATTTGCAAGAACCACAGGAGTCCCTTCCAAGAATTTCAGTTAAAGCAAAGTCTTCTGAAACAGGAGACAATCAGAGTCCCCATTCTCCAGGTCTGAGAAGAAGATTTACTCCTAGTGCAGATTCTTTGTCACCTGTCAAACTTGACGCTGCAGCTCAAGCGCACATTGAAAAGCATAGAAGACTTCAGGAGGATTTGACTGATGAAATGGTTGGGTTGGCAAGGCAACTCAAAGAGACCAGTCTCATGATGAGCCACTCCGTGCAAAACACTGAAAAACTACTTGACTCTACAGAGGAGGCTGTAGAAAGGAGCTTGGCTAGCACTGGTCATGCCACTGCACGGGCATCCGATATATACTCAAAGACCTCCAAGACTTCATGTTTCACATGGCTTTTGATGTTTCTAATGACGTGTATATTCATTATGGTGGTACTTTTAATTCGTGTCACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 map04130
Pfam Domains
Protein Families
Use1

Protein Analysis

211

Amino Acids

23.41

Weight (kDa)

7.8

Isoelectric Point (pI)

63.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Use1 PF09753 7 - 211 7.8e-47 Membrane fusion protein Use1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 213
AcuI CTGAAG 2 cut(s) 96, 350
AdeI CACNNNGTG 1 cut(s) 442
AfaI GTAC 1 cut(s) 615
AfiI CCNNNNNNNGG 2 cut(s) 58, 522
AflII CTTAAG 1 cut(s) 121
AflIII ACRYGT 1 cut(s) 593
AgsI TTSAA 2 cut(s) 111, 350
AjiI CACGTC 1 cut(s) 594
AjnI CCWGG 1 cut(s) 268
AluBI AGCT 6 cut(s) 47, 70, 74, 167, 335, 495
AluI AGCT 6 cut(s) 47, 70, 74, 167, 335, 495
Alw26I GTCTC 3 cut(s) 240, 410, 428
ApeKI GCWGC 5 cut(s) 37, 44, 47, 329, 332
ApoI RAATTY 1 cut(s) 213
AspLEI GCGC 1 cut(s) 343
AsuHPI GGTGA 2 cut(s) 34, 303
AsuII TTCGAA 1 cut(s) 158
AsuNHI GCTAGC 2 cut(s) 70, 500
BarI GAAGNNNNNNTAC 2 cut(s) 271, 303
BbsI GAAGAC 2 cut(s) 225, 367
BbvI GCAGC 5 cut(s) 24, 34, 56, 316, 344
BcgI CGANNNNNNTGC 2 cut(s) 134, 168
BciT130I CCWGG 1 cut(s) 270
BcoDI GTCTC 3 cut(s) 240, 410, 428
BfaI CTAG 4 cut(s) 71, 102, 294, 501
BfmI CTRYAG 3 cut(s) 330, 471, 483
BfrI CTTAAG 1 cut(s) 121
BisI GCNGC 5 cut(s) 38, 45, 48, 330, 333
BlsI GCNGC 5 cut(s) 39, 46, 49, 331, 334
Bme1390I CCNGG 1 cut(s) 270
BmgBI CACGTC 1 cut(s) 594
BmrFI CCNGG 1 cut(s) 270
BmsI GCATC 1 cut(s) 536
BmtI GCTAGC 2 cut(s) 74, 504
BpiI GAAGAC 2 cut(s) 225, 367
BpmI CTGGAG 1 cut(s) 252
Bpu14I TTCGAA 1 cut(s) 158
BpuEI CTTGAG 2 cut(s) 49, 321
BsaI GGTCTC 1 cut(s) 410
BsaWI WCCGGW 1 cut(s) 88
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 522
Bse1I ACTGG 2 cut(s) 420, 511
Bse3DI GCAATG 2 cut(s) 150, 173
BseBI CCWGG 1 cut(s) 270
BseGI GGATG 1 cut(s) 527
BseLI CCNNNNNNNGG 2 cut(s) 58, 522
BseMI GCAATG 2 cut(s) 150, 173
BseMII CTCAG 1 cut(s) 266
BseNI ACTGG 2 cut(s) 420, 511
BseRI GAGGAG 2 cut(s) 46, 491
BseXI GCAGC 5 cut(s) 24, 34, 56, 316, 344
BsgI GTGCAG 2 cut(s) 318, 504
BsiSI CCGG 1 cut(s) 89
BslFI GGGAC 2 cut(s) 187, 243
BslI CCNNNNNNNGG 2 cut(s) 58, 522
BsmAI GTCTC 3 cut(s) 240, 410, 428
BsmFI GGGAC 2 cut(s) 187, 243
Bso31I GGTCTC 1 cut(s) 410
Bsp119I TTCGAA 1 cut(s) 158
Bsp143I GATC 1 cut(s) 141
BspCNI CTCAG 1 cut(s) 267
BspHI TCATGA 1 cut(s) 426
BspMAI CTGCAG 1 cut(s) 334
BspOI GCTAGC 2 cut(s) 74, 504
BspT104I TTCGAA 1 cut(s) 158
BspTI CTTAAG 1 cut(s) 121
BspTNI GGTCTC 1 cut(s) 410
BsrDI GCAATG 2 cut(s) 150, 173
BsrI ACTGG 2 cut(s) 420, 511
BssMI GATC 1 cut(s) 141
Bst2UI CCWGG 1 cut(s) 270
Bst6I CTCTTC 1 cut(s) 72
BstAFI CTTAAG 1 cut(s) 121
BstBI TTCGAA 1 cut(s) 158
BstC8I GCNNGC 3 cut(s) 42, 72, 502
BstDEI CTNAG 2 cut(s) 275, 633
BstENI CCTNNNNNAGG 1 cut(s) 56
BstF5I GGATG 1 cut(s) 527
BstHHI GCGC 1 cut(s) 343
BstKTI GATC 1 cut(s) 144
BstMAI GTCTC 3 cut(s) 240, 410, 428
BstMBI GATC 1 cut(s) 141
BstMWI GCNNNNNNNGC 3 cut(s) 184, 338, 501
BstNI CCWGG 1 cut(s) 270
BstSCI CCNGG 1 cut(s) 268
BstSFI CTRYAG 3 cut(s) 330, 471, 483
BstV1I GCAGC 5 cut(s) 24, 34, 56, 316, 344
BstV2I GAAGAC 2 cut(s) 225, 367
BtrI CACGTC 1 cut(s) 594
BtsCI GGATG 1 cut(s) 527
BtsI GCAGTG 1 cut(s) 516
BtsIMutI CAGTG 3 cut(s) 450, 504, 516
Cac8I GCNNGC 3 cut(s) 42, 72, 502
CciI TCATGA 1 cut(s) 426
CfoI GCGC 1 cut(s) 343
CseI GACGC 1 cut(s) 335
Csp6I GTAC 1 cut(s) 614
CviAII CATG 4 cut(s) 427, 512, 561, 570
CviQI GTAC 1 cut(s) 614
DdeI CTNAG 2 cut(s) 275, 633
DpnI GATC 1 cut(s) 143
DpnII GATC 1 cut(s) 141
DraIII CACNNNGTG 1 cut(s) 442
Eam1104I CTCTTC 1 cut(s) 72
EarI CTCTTC 1 cut(s) 72
Eco31I GGTCTC 1 cut(s) 410
Eco57I CTGAAG 2 cut(s) 96, 350
EcoNI CCTNNNNNAGG 1 cut(s) 56
EcoRII CCWGG 1 cut(s) 268
FaeI CATG 4 cut(s) 430, 515, 564, 573
FaiI YATR 9 cut(s) 357, 428, 513, 536, 538, 562, 571, 599, 608
FalI AAGNNNNNCTT 2 cut(s) 104, 136
FaqI GGGAC 2 cut(s) 187, 243
FatI CATG 4 cut(s) 426, 511, 560, 569
Fnu4HI GCNGC 5 cut(s) 38, 45, 48, 330, 333
FokI GGATG 1 cut(s) 514
Fsp4HI GCNGC 5 cut(s) 38, 45, 48, 330, 333
FspBI CTAG 4 cut(s) 71, 102, 294, 501
GlaI GCGC 1 cut(s) 342
GluI GCNGC 5 cut(s) 38, 45, 48, 330, 333
GsuI CTGGAG 1 cut(s) 252
HapII CCGG 1 cut(s) 89
HgaI GACGC 1 cut(s) 335
HhaI GCGC 1 cut(s) 343
Hin1II CATG 4 cut(s) 430, 515, 564, 573
Hin6I GCGC 1 cut(s) 341
HinP1I GCGC 1 cut(s) 341
HinfI GANTC 4 cut(s) 200, 256, 302, 467
HpaII CCGG 1 cut(s) 89
HphI GGTGA 2 cut(s) 34, 303
Hpy166II GTNNAC 1 cut(s) 25
Hpy188I TCNGA 5 cut(s) 136, 238, 255, 276, 532
Hpy188III TCNNGA 2 cut(s) 368, 427
Hpy8I GTNNAC 1 cut(s) 25
HpyAV CCTTC 2 cut(s) 86, 215
HpyCH4IV ACGT 1 cut(s) 593
HpyCH4V TGCA 6 cut(s) 44, 187, 299, 332, 445, 521
HpyF10VI GCNNNNNNNGC 3 cut(s) 184, 338, 501
HpyF3I CTNAG 2 cut(s) 275, 633
HpySE526I ACGT 1 cut(s) 593
Hsp92II CATG 4 cut(s) 430, 515, 564, 573
HspAI GCGC 1 cut(s) 341
Kzo9I GATC 1 cut(s) 141
LmnI GCTCC 1 cut(s) 492
Lsp1109I GCAGC 5 cut(s) 24, 34, 56, 316, 344
LweI GCATC 1 cut(s) 536
MaeI CTAG 4 cut(s) 71, 102, 294, 501
MaeII ACGT 1 cut(s) 593
MaeIII GTNAC 3 cut(s) 125, 309, 628
MalI GATC 1 cut(s) 143
MboI GATC 1 cut(s) 141
MboII GAAGA 6 cut(s) 89, 151, 225, 291, 294, 372
MluCI AATT 3 cut(s) 161, 213, 621
MlyI GAGTC 3 cut(s) 209, 265, 461
MnlI CCTC 8 cut(s) 13, 24, 27, 62, 364, 469, 472, 559
MseI TTAA 3 cut(s) 122, 222, 620
MslI CAYNNNNRTG 1 cut(s) 608
MspA1I CMGCKG 1 cut(s) 47
MspCI CTTAAG 1 cut(s) 121
MspI CCGG 1 cut(s) 89
MspR9I CCNGG 1 cut(s) 270
MvaI CCWGG 1 cut(s) 270
MwoI GCNNNNNNNGC 3 cut(s) 184, 338, 501
NdeII GATC 1 cut(s) 141
NheI GCTAGC 2 cut(s) 70, 500
NlaIII CATG 4 cut(s) 430, 515, 564, 573
NmuCI GTSAC 3 cut(s) 125, 309, 628
NspV TTCGAA 1 cut(s) 158
PagI TCATGA 1 cut(s) 426
PfeI GAWTC 1 cut(s) 302
PkrI GCNGC 5 cut(s) 39, 46, 49, 331, 334
PleI GAGTC 3 cut(s) 208, 264, 461
PpsI GAGTC 3 cut(s) 208, 264, 461
Psp6I CCWGG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 268
PstI CTGCAG 1 cut(s) 334
PvuII CAGCTG 1 cut(s) 47
RsaI GTAC 1 cut(s) 615
RsaNI GTAC 1 cut(s) 614
RseI CAYNNNNRTG 1 cut(s) 608
SaqAI TTAA 3 cut(s) 122, 222, 620
SatI GCNGC 5 cut(s) 38, 45, 48, 330, 333
Sau3AI GATC 1 cut(s) 141
SchI GAGTC 3 cut(s) 209, 265, 461
ScrFI CCNGG 1 cut(s) 270
SfaNI GCATC 1 cut(s) 536
SfcI CTRYAG 3 cut(s) 330, 471, 483
SfuI TTCGAA 1 cut(s) 158
SmiMI CAYNNNNRTG 1 cut(s) 608
SmlI CTYRAG 3 cut(s) 28, 121, 336
SmoI CTYRAG 3 cut(s) 28, 121, 336
Sse9I AATT 3 cut(s) 161, 213, 621
SspMI CTAG 4 cut(s) 71, 102, 294, 501
StyD4I CCNGG 1 cut(s) 268
TaiI ACGT 1 cut(s) 596
TaqI TCGA 2 cut(s) 144, 158
TasI AATT 3 cut(s) 161, 213, 621
TfiI GAWTC 1 cut(s) 302
Tru1I TTAA 3 cut(s) 122, 222, 620
Tru9I TTAA 3 cut(s) 122, 222, 620
TscAI CASTG 3 cut(s) 457, 511, 523
TseFI GTSAC 3 cut(s) 125, 309, 628
TseI GCWGC 5 cut(s) 37, 44, 47, 329, 332
Tsp45I GTSAC 3 cut(s) 125, 309, 628
TspDTI ATGAA 4 cut(s) 17, 399, 549, 592
TspGWI ACGGA 1 cut(s) 430
TspRI CASTG 3 cut(s) 457, 511, 523
Vha464I CTTAAG 1 cut(s) 121
XagI CCTNNNNNAGG 1 cut(s) 56
XapI RAATTY 1 cut(s) 213
XspI CTAG 4 cut(s) 71, 102, 294, 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.