pycom13g21130

Vesicle transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0003166
Physical Location & Seq
Forward (+)
17352239 .. 17355521
3283 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g21130.1

Sequence Viewer

Length: 720 bp
ATGAGAATCAGTAAAACAGAGGTGAATTTGAAGAGGCTGCTTGCAGCTGCGCCTCAGCAACAGAACCAGGCAAAACTTGTACATTATGTTGCTACTTTACGAGAACTGGTAGAACAACTAGCTGAAGAGAGAACTCCTGAAGGCTTACCAAGAGTTTCAAAGGCTGTTTTGAGTGACTATTCAGAGAAGATTGAAGCCATTGCTTCCAAATTAGCTGCTCCACCACCTGATATTCAAACGCCCGAAGAGCCCCTTGCAAGAATTTCTGTCAAAGCAAACTCTTCTGATACAGGAGACAATCAGATTCCCCCTTCTCCAGGTTTGAGAAGGAGATTTGTGCCTATCTCAAGTACTGAAGATGGAACTTGTGAGACTGCTGGTGTTGATTCTTCAGGGCCTGTCAAACTGGATGCTGCAGCCCAAGCACACATTGAAAAGCATAGAAAGCTTCAAGAGGATTTGACGGATGAAATGGTTGGATTGGCGAGGCAGCTCAAAGAGAGTAGTCTCATGATGAGTCACTCCCTGGAAAACACTGAAAAAATACTTGATTCTACAGAGAAGGCTGTTGAAAGTAGCTTGGCAAGCACTGGTCACGCCACTACACGGGCAGCAGGTATATACTCGAAGACGTCCAAGACTACTTGTTTCACATGGCTTCTGATGTTTGTCATGACATGTGTATTTATCATGGTGGTTCTTTTAATTCGCGTCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 map04130
Pfam Domains
Protein Families
Use1

Protein Analysis

240

Amino Acids

26.11

Weight (kDa)

6.24

Isoelectric Point (pI)

51.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Use1 PF09753 5 - 239 5.9e-68 Membrane fusion protein Use1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 635
Acc36I ACCTGC 1 cut(s) 605
AccII CGCG 1 cut(s) 711
AcsI RAATTY 2 cut(s) 25, 261
AcuI CTGAAG 4 cut(s) 144, 159, 375, 375
AcyI GRCGYC 1 cut(s) 632
AfaI GTAC 2 cut(s) 81, 352
AfiI CCNNNNNNNGG 2 cut(s) 317, 606
AflIII ACRYGT 1 cut(s) 677
AgsI TTSAA 7 cut(s) 31, 159, 194, 236, 434, 452, 572
AjnI CCWGG 3 cut(s) 66, 316, 525
AluBI AGCT 6 cut(s) 47, 122, 215, 448, 493, 579
AluI AGCT 6 cut(s) 47, 122, 215, 448, 493, 579
Alw26I GTCTC 3 cut(s) 288, 365, 512
AlwNI CAGNNNCTG 1 cut(s) 398
AoxI GGCC 1 cut(s) 395
ApeKI GCWGC 8 cut(s) 37, 44, 47, 215, 413, 416, 490, 611
ApoI RAATTY 2 cut(s) 25, 261
AspLEI GCGC 1 cut(s) 52
AspS9I GGNCC 1 cut(s) 395
AsuHPI GGTGA 1 cut(s) 34
BanII GRGCYC 1 cut(s) 252
BbsI GAAGAC 1 cut(s) 635
BbvCI CCTCAGC 1 cut(s) 54
BbvI GCAGC 8 cut(s) 24, 34, 56, 202, 400, 428, 502, 623
BccI CCATC 1 cut(s) 353
BciT130I CCWGG 3 cut(s) 68, 318, 527
BcoDI GTCTC 3 cut(s) 288, 365, 512
BfaI CTAG 1 cut(s) 119
BfmI CTRYAG 2 cut(s) 414, 555
BfuAI ACCTGC 1 cut(s) 605
BisI GCNGC 8 cut(s) 38, 45, 48, 216, 414, 417, 491, 612
BlsI GCNGC 8 cut(s) 39, 46, 49, 217, 415, 418, 492, 613
BmcAI AGTACT 1 cut(s) 352
Bme1390I CCNGG 3 cut(s) 68, 318, 527
BmgT120I GGNCC 1 cut(s) 395
BmrFI CCNGG 3 cut(s) 68, 318, 527
BmsI GCATC 1 cut(s) 400
BpiI GAAGAC 1 cut(s) 635
BpmI CTGGAG 1 cut(s) 300
Bpu10I CCTNAGC 1 cut(s) 54
BpuEI CTTGAG 1 cut(s) 331
BsaHI GRCGYC 1 cut(s) 632
BsaJI CCNNGG 1 cut(s) 525
Bsc4I CCNNNNNNNGG 2 cut(s) 317, 606
Bse1I ACTGG 3 cut(s) 111, 411, 595
Bse3DI GCAATG 1 cut(s) 198
BseBI CCWGG 3 cut(s) 68, 318, 527
BseDI CCNNGG 1 cut(s) 525
BseGI GGATG 2 cut(s) 415, 472
BseLI CCNNNNNNNGG 2 cut(s) 317, 606
BseMI GCAATG 1 cut(s) 198
BseMII CTCAG 1 cut(s) 68
BseNI ACTGG 3 cut(s) 111, 411, 595
BseXI GCAGC 8 cut(s) 24, 34, 56, 202, 400, 428, 502, 623
Bsh1236I CGCG 1 cut(s) 711
BshFI GGCC 1 cut(s) 397
BslI CCNNNNNNNGG 2 cut(s) 317, 606
BsmAI GTCTC 3 cut(s) 288, 365, 512
BsnI GGCC 1 cut(s) 397
Bsp1286I GDGCHC 1 cut(s) 252
Bsp1407I TGTACA 1 cut(s) 79
BspANI GGCC 1 cut(s) 397
BspCNI CTCAG 1 cut(s) 67
BspFNI CGCG 1 cut(s) 711
BspHI TCATGA 2 cut(s) 510, 672
BspMAI CTGCAG 1 cut(s) 418
BspMI ACCTGC 1 cut(s) 605
BspQI GCTCTTC 1 cut(s) 240
BsrDI GCAATG 1 cut(s) 198
BsrGI TGTACA 1 cut(s) 79
BsrI ACTGG 3 cut(s) 111, 411, 595
BssECI CCNNGG 1 cut(s) 525
BssNI GRCGYC 1 cut(s) 632
Bst2UI CCWGG 3 cut(s) 68, 318, 527
Bst6I CTCTTC 4 cut(s) 26, 120, 240, 286
BstACI GRCGYC 1 cut(s) 632
BstAUI TGTACA 1 cut(s) 79
BstC8I GCNNGC 2 cut(s) 42, 586
BstDEI CTNAG 1 cut(s) 54
BstENI CCTNNNNNAGG 1 cut(s) 315
BstF5I GGATG 2 cut(s) 415, 472
BstFNI CGCG 1 cut(s) 711
BstHHI GCGC 1 cut(s) 52
BstMAI GTCTC 3 cut(s) 288, 365, 512
BstMWI GCNNNNNNNGC 4 cut(s) 247, 422, 445, 585
BstNI CCWGG 3 cut(s) 68, 318, 527
BstNSI RCATGY 1 cut(s) 681
BstSCI CCNGG 3 cut(s) 66, 316, 525
BstSFI CTRYAG 2 cut(s) 414, 555
BstUI CGCG 1 cut(s) 711
BstV1I GCAGC 8 cut(s) 24, 34, 56, 202, 400, 428, 502, 623
BstV2I GAAGAC 1 cut(s) 635
BsuRI GGCC 1 cut(s) 397
BtsCI GGATG 2 cut(s) 415, 472
BtsIMutI CAGTG 2 cut(s) 534, 588
BveI ACCTGC 1 cut(s) 605
Cac8I GCNNGC 2 cut(s) 42, 586
CaiI CAGNNNCTG 1 cut(s) 398
CciI TCATGA 2 cut(s) 510, 672
CfoI GCGC 1 cut(s) 52
Cfr13I GGNCC 1 cut(s) 395
CseI GACGC 1 cut(s) 700
Csp6I GTAC 2 cut(s) 80, 351
CviAII CATG 5 cut(s) 511, 654, 673, 678, 691
CviQI GTAC 2 cut(s) 80, 351
DdeI CTNAG 1 cut(s) 54
Eam1104I CTCTTC 4 cut(s) 26, 120, 240, 286
EarI CTCTTC 4 cut(s) 26, 120, 240, 286
Eco24I GRGCYC 1 cut(s) 252
Eco57I CTGAAG 4 cut(s) 144, 159, 375, 375
EcoNI CCTNNNNNAGG 1 cut(s) 315
EcoO109I RGGNCCY 1 cut(s) 395
EcoRII CCWGG 3 cut(s) 66, 316, 525
EcoT38I GRGCYC 1 cut(s) 252
FaeI CATG 5 cut(s) 514, 657, 676, 681, 694
FaiI YATR 9 cut(s) 87, 441, 512, 620, 622, 655, 674, 679, 692
FalI AAGNNNNNCTT 2 cut(s) 237, 269
FatI CATG 5 cut(s) 510, 653, 672, 677, 690
Fnu4HI GCNGC 8 cut(s) 38, 45, 48, 216, 414, 417, 491, 612
FokI GGATG 2 cut(s) 422, 479
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 8 cut(s) 38, 45, 48, 216, 414, 417, 491, 612
FspBI CTAG 1 cut(s) 119
GlaI GCGC 1 cut(s) 51
GluI GCNGC 8 cut(s) 38, 45, 48, 216, 414, 417, 491, 612
GsuI CTGGAG 1 cut(s) 300
HaeIII GGCC 1 cut(s) 397
HgaI GACGC 1 cut(s) 700
HhaI GCGC 1 cut(s) 52
Hin1I GRCGYC 1 cut(s) 632
Hin1II CATG 5 cut(s) 514, 657, 676, 681, 694
Hin6I GCGC 1 cut(s) 50
HinP1I GCGC 1 cut(s) 50
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 5 cut(s) 6, 304, 386, 517, 551
HphI GGTGA 1 cut(s) 34
Hpy188I TCNGA 4 cut(s) 184, 286, 303, 663
Hpy188III TCNNGA 4 cut(s) 137, 452, 511, 673
HpyAV CCTTC 4 cut(s) 134, 321, 321, 556
HpyCH4IV ACGT 1 cut(s) 632
HpyCH4V TGCA 3 cut(s) 44, 257, 416
HpyF10VI GCNNNNNNNGC 4 cut(s) 247, 422, 445, 585
HpyF3I CTNAG 1 cut(s) 54
HpySE526I ACGT 1 cut(s) 632
Hsp92I GRCGYC 1 cut(s) 632
Hsp92II CATG 5 cut(s) 514, 657, 676, 681, 694
HspAI GCGC 1 cut(s) 50
LguI GCTCTTC 1 cut(s) 240
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 8 cut(s) 24, 34, 56, 202, 400, 428, 502, 623
LweI GCATC 1 cut(s) 400
MaeI CTAG 1 cut(s) 119
MaeII ACGT 1 cut(s) 632
MaeIII GTNAC 4 cut(s) 173, 518, 593, 712
MboII GAAGA 8 cut(s) 43, 137, 199, 257, 273, 368, 381, 640
MhlI GDGCHC 1 cut(s) 252
MluCI AATT 4 cut(s) 25, 209, 261, 705
MlyI GAGTC 1 cut(s) 526
MmeI TCCRAC 1 cut(s) 457
MnlI CCTC 5 cut(s) 13, 27, 63, 448, 480
MseI TTAA 2 cut(s) 704, 718
MspA1I CMGCKG 1 cut(s) 47
MspR9I CCNGG 3 cut(s) 68, 318, 527
MvaI CCWGG 3 cut(s) 68, 318, 527
MvnI CGCG 1 cut(s) 711
MwoI GCNNNNNNNGC 4 cut(s) 247, 422, 445, 585
NlaIII CATG 5 cut(s) 514, 657, 676, 681, 694
NmuCI GTSAC 4 cut(s) 173, 518, 593, 712
NspI RCATGY 1 cut(s) 681
PagI TCATGA 2 cut(s) 510, 672
PciI ACATGT 1 cut(s) 677
PciSI GCTCTTC 1 cut(s) 240
PfeI GAWTC 4 cut(s) 6, 304, 386, 551
PkrI GCNGC 8 cut(s) 39, 46, 49, 217, 415, 418, 492, 613
PleI GAGTC 1 cut(s) 525
PpsI GAGTC 1 cut(s) 525
PscI ACATGT 1 cut(s) 677
Psp6I CCWGG 3 cut(s) 66, 316, 525
PspGI CCWGG 3 cut(s) 66, 316, 525
PspPI GGNCC 1 cut(s) 395
PstI CTGCAG 1 cut(s) 418
PstNI CAGNNNCTG 1 cut(s) 398
PvuII CAGCTG 1 cut(s) 47
RsaI GTAC 2 cut(s) 81, 352
RsaNI GTAC 2 cut(s) 80, 351
SapI GCTCTTC 1 cut(s) 240
SaqAI TTAA 2 cut(s) 704, 718
SatI GCNGC 8 cut(s) 38, 45, 48, 216, 414, 417, 491, 612
Sau96I GGNCC 1 cut(s) 395
ScaI AGTACT 1 cut(s) 352
SchI GAGTC 1 cut(s) 526
ScrFI CCNGG 3 cut(s) 68, 318, 527
SduI GDGCHC 1 cut(s) 252
SfaNI GCATC 1 cut(s) 400
SfcI CTRYAG 2 cut(s) 414, 555
SmlI CTYRAG 1 cut(s) 346
SmoI CTYRAG 1 cut(s) 346
Sse9I AATT 4 cut(s) 25, 209, 261, 705
SspMI CTAG 1 cut(s) 119
StyD4I CCNGG 3 cut(s) 66, 316, 525
TaiI ACGT 1 cut(s) 635
TaqI TCGA 1 cut(s) 626
TasI AATT 4 cut(s) 25, 209, 261, 705
TatI WGTACW 2 cut(s) 79, 350
TfiI GAWTC 4 cut(s) 6, 304, 386, 551
Tru1I TTAA 2 cut(s) 704, 718
Tru9I TTAA 2 cut(s) 704, 718
TscAI CASTG 2 cut(s) 541, 595
TseFI GTSAC 4 cut(s) 173, 518, 593, 712
TseI GCWGC 8 cut(s) 37, 44, 47, 215, 413, 416, 490, 611
Tsp45I GTSAC 4 cut(s) 173, 518, 593, 712
TspDTI ATGAA 1 cut(s) 483
TspGWI ACGGA 1 cut(s) 479
TspRI CASTG 2 cut(s) 541, 595
XagI CCTNNNNNAGG 1 cut(s) 315
XapI RAATTY 2 cut(s) 25, 261
XceI RCATGY 1 cut(s) 681
XspI CTAG 1 cut(s) 119
ZraI GACGTC 1 cut(s) 633
ZrmI AGTACT 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.