AT1G59870
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002725
Physical Location & Seq
Forward (+)
22034423 .. 22040126
5704 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G59870.1

Sequence Viewer

Length: 4410 bp
ATGGATTACAATCCAAATCTTCCTCCTTTAGGAGGAGGTGGTGTTAGTATGAGAAGAAGCATAAGTCGAAGTGTAAGCAGAGCAAGTAGGAACATTGAAGATATCTTCTCATCTGGTTCAAGAAGAACACAATCAGTCAACGACGATGAAGAAGCTCTTAAATGGGCTGCCATTGAGAAGCTACCAACTTACAGTCGTCTCCGAACCACTCTCATGAACGCTGTAGTCGAAGACGATGTTTACGGTAACCAGCTCATGAGCAAGGAGGTTGATGTAACCAAGCTTGATGGTGAAGATCGTCAGAAGTTTATTGACATGGTTTTCAAAGTAGCTGAGCAAGATAATGAAAGGATCTTGACTAAGCTAAGAAACAGGATCGATAGAGTTGGTATCAAACTTCCAACTGTTGAAGTCAGGTACGAGCATTTGACGATTAAAGCTGATTGTTACACTGGTAATAGATCTCTTCCTACACTTTTGAATGTTGTGAGGAACATGGGAGAGTCTGCTTTAGGTATGATTGGTATTCAATTTGCTAAGAAAGCTCAGCTTACGATTCTTAAAGATATCTCTGGGGTTATTAAACCTGGAAGGATGACACTTTTGTTGGGTCCTCCTTCTTCTGGTAAGACCACTCTTTTGTTGGCTTTAGCTGGGAAACTTGATAAATCTCTACAAGTCAGTGGTGATATTACTTACAATGGTTACCAACTCGATGAGTTTGTTCCGAGAAAGACCTCTGCTTACATTAGTCAGAACGATCTTCATGTTGGTATCATGACTGTTAAGGAGACTCTTGACTTCTCTGCTAGGTGTCAAGGTGTTGGTACTCGTTATGATCTGTTGAATGAGCTTGCGAGGAGAGAAAAGGACGCTGGTATATTCCCGGAAGCCGATGTTGATCTCTTCATGAAAGCTTCTGCTGCTCAAGGTGTTAAGAACAGTCTCGTCACTGATTATACTCTCAAAATTTTGGGGCTTGACATTTGCAAAGACACAATAGTTGGAGATGACATGATGAGAGGTATATCTGGAGGTCAGAAGAAACGTGTCACAACTGGTGAGATGATTGTTGGACCTACTAAGACACTCTTCATGGACGAAATATCCACTGGTCTTGACAGTTCCACTACTTTCCAAATCGTCAAGTGTCTGCAACAAATCGTTCACCTCAATGAAGCCACGGTGCTCATGTCTCTCCTCCAGCCTGCTCCTGAGACTTTTGATTTATTCGATGATATCATCTTGGTGTCGGAAGGTCAGATCGTGTACCAAGGACCGAGAGACAACATTCTTGAGTTCTTTGAGAGCTTTGGGTTCAAGTGTCCTGAGAGAAAAGGAACAGCTGATTTCCTGCAAGAGGTTACTTCCAAGAAAGATCAAGAACAGTACTGGGTGAACCCGAACAGACCTTATCACTACATTCCGGTTTCAGAGTTTGCCAGTAGATACAAGAGTTTCCATGTTGGGACGAAGATGTCTAACGAACTTGCAGTACCGTTCGATAAGTCTCGCGGCCACAAAGCAGCTCTTGTGTTCGATAAGTACTCTGTCTCAAAGAGGGAGCTTCTCAAGAGCTGTTGGGACAAAGAGTGGCTGCTTATGCAGCGAAACGCGTTCTTCTATGTTTTCAAGACTGTCCAGATCGTCATCATTGCTGCAATCACGTCTACACTCTTCCTGAGAACCGAAATGAACACAAGAAACGAGGGTGATGCTAATCTCTACATAGGAGCATTGCTATTTGGAATGATCATCAACATGTTTAATGGGTTTGCGGAGATGGCTATGATGGTTTCAAGACTCCCTGTGTTCTACAAACAGAGGGATCTCTTGTTTTATCCATCCTGGACCTTCTCACTTCCCACTTTCTTGCTTGGGATTCCAAGCTCAATATTAGAATCGACGGCTTGGATGGTGGTGACTTATTACTCCATTGGTTTTGCACCTGACGCCAGCCGCTTCTTCAAGCAGTTTCTTCTGGTGTTTCTGATTCAACAAATGGCTGCATCCCTCTTTAGGTTGATTGCTTCTGTGTGCAGAACCATGATGATTGCTAATACTGGTGGTGCTCTCACTCTACTTCTTGTGTTCTTGCTCGGAGGCTTCCTTCTTCCGAAAGGCAAGATTCCTGACTGGTGGGGTTGGGCTTACTGGGTATCTCCTCTCACCTATGCTTTCAACGGTCTAGTAGTCAATGAAATGTTTGCTCCCAGATGGATGAACAAAATGGCTTCTTCTAACAGCACAATAAAGCTTGGAACTATGGTGCTTAATACTTGGGATGTCTACCATCAAAAGAACTGGTACTGGATTTCAGTTGGAGCCTTGCTTTGTTTCACAGCCCTCTTCAACATTCTATTCACCTTGGCACTTACCTATCTCAACCCTCTTGGGAAGAAGGCAGGTTTACTTCCAGAAGAAGAAAATGAAGACGCTGATCAGGGGAAAGATCCAATGCGTAGATCTTTGTCTACTGCAGATGGGAACAGAAGAGGAGAGGTCGCAATGGGGAGAATGAGTAGGGACTCTGCGGCTGAAGCATCAGGTGGTGCAGGCAATAAGAAAGGAATGGTTCTTCCTTTCACTCCTTTAGCTATGTCCTTTGACGACGTCAAATACTTTGTTGACATGCCTGGGGAAATGAGAGACCAAGGAGTTACAGAAACAAGACTCCAACTGCTTAAAGGTGTGACTGGTGCATTTAGGCCAGGAGTTTTGACTGCGCTTATGGGAGTGAGTGGTGCCGGTAAGACTACGCTTATGGACGTTTTGGCCGGAAGGAAAACAGGTGGATACATTGAAGGAGATGTGAGAATATCAGGATTCCCAAAGGTTCAAGAAACATTTGCTAGAATCTCAGGATATTGTGAGCAGACCGATATTCACTCCCCGCAAGTAACAGTCAGAGAATCTCTGATTTTCTCTGCTTTCCTTCGTCTTCCTAAAGAAGTCGGCAAAGATGAAAAAATGATGTTTGTGGATCAAGTGATGGAATTGGTAGAGCTGGACAGTCTTAGGGACTCCATTGTTGGTTTACCGGGTGTCACGGGGCTTTCCACGGAGCAGAGAAAGAGACTGACAATCGCGGTGGAGCTTGTAGCCAACCCTTCCATCATCTTTATGGATGAGCCAACTTCAGGGCTAGACGCTAGAGCAGCGGCTATTGTGATGAGGGCGGTAAGGAACACAGTGGACACTGGAAGAACCGTGGTCTGCACCATTCATCAGCCTAGCATTGATATCTTTGAAGCATTTGATGAATTGATGCTGATGAAGAGAGGAGGACAAGTGATTTACGCGGGTCCATTGGGTCAAAACTCTCACAAGGTGGTTGAGTACTTTGAATCTTTCCCCGGAGTGTCCAAGATTCCAGAAAAGTATAACCCGGCCACTTGGATGCTCGAAGCTAGCTCACTCGCCGCTGAGCTAAAGCTTAGTGTTGACTTTGCTGAGTTATACAATCAATCAGCATTGCACCAGCGAAACAAAGCGTTGGTAAAAGAACTAAGTGTACCACCAGCAGGAGCATCAGATCTTTACTTTGCTACACAATTCTCACAAAACACATGGGGACAGTTCAAATCATGCTTATGGAAACAATGGTGGACGTATTGGAGATCTCCAGACTACAATCTTGTCCGTTTCATCTTCACATTGGCAACATCTCTCTTGATTGGTACAGTCTTCTGGCAAATCGGAGGTAACAGGTCGAACGCAGGGGATCTAACAATGGTGATAGGAGCATTGTATGCCGCGATTATCTTCGTGGGAATCAACAACTGTTCAACAGTACAACCGATGGTTGCAGTGGAAAGAACAGTGTTCTACAGAGAAAGAGCAGCAGGAATGTACTCAGCCATGCCATATGCCATCTCTCAAGTCACTTGTGAGCTTCCCTATGTCCTTATTCAAACCGTTTACTACTCACTCATCGTCTACGCCATGGTTGGTTTCGAATGGAAAGCCGAAAAGTTCTTCTGGTTCGTCTTCGTTAGCTACTTCTCATTCCTCTACTGGACTTACTACGGCATGATGACTGTTTCCCTCACACCAAACCAACAAGTCGCTTCGATTTTCGCCTCAGCGTTTTACGGTATTTTCAACCTCTTCTCTGGTTTCTTCATTCCAAGACCCAAAATCCCAAAATGGTGGATTTGGTACTACTGGATCTGCCCTGTTGCATGGACCGTGTATGGATTGATAGTGTCGCAGTACGGTGATGTGGAGACACGTATCCAAGTCCTTGGTGGTGCTCCTGACTTAACCGTCAAGCAATACATTGAGGACCATTATGGTTTCCAATCTGACTTTATGGGACCAGTGGCGGCTGTACTCATCGCTTTCACCGTCTTCTTCGCCTTCATCTTCGCCTTCTGCATCAGAACTCTCAACTTCCAGACCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000041 GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005886 GO:0006082 GO:0006725 GO:0006790 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006855 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008324 GO:0009056 GO:0009507 GO:0009526 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009719 GO:0009725 GO:0009733 GO:0009737 GO:0009814 GO:0009817 GO:0009889 GO:0009941 GO:0009987 GO:0010033 GO:0010243 GO:0014070 GO:0015075 GO:0015086 GO:0015318 GO:0015399 GO:0015405 GO:0015691 GO:0015850 GO:0015893 GO:0016020 GO:0016021 GO:0016043 GO:0016143 GO:0016145 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019439 GO:0019748 GO:0019757 GO:0019759 GO:0019760 GO:0019762 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031323 GO:0031326 GO:0031347 GO:0031348 GO:0031967 GO:0031975 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0033993 GO:0034220 GO:0034641 GO:0042221 GO:0042343 GO:0042344 GO:0042430 GO:0042436 GO:0042493 GO:0042545 GO:0042623 GO:0042626 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043455 GO:0043492 GO:0044237 GO:0044248 GO:0044270 GO:0044273 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045229 GO:0046483 GO:0046700 GO:0046873 GO:0046915 GO:0048519 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0055085 GO:0065007 GO:0070574 GO:0070727 GO:0070838 GO:0070887 GO:0071229 GO:0071310 GO:0071365 GO:0071366 GO:0071407 GO:0071417 GO:0071495 GO:0071554 GO:0071555 GO:0071702 GO:0071704 GO:0071840 GO:0071944 GO:0072511 GO:0080026 GO:0080134 GO:0097305 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:1900376 GO:1901135 GO:1901136 GO:1901140 GO:1901141 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575 GO:1901657 GO:1901658 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:2000762
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1469

Amino Acids

165.08

Weight (kDa)

8.08

Isoelectric Point (pI)

33.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 112 - 162 9.6e-08 ABC-transporter N-terminal
ABC_tran PF00005 187 - 368 2.5e-14 ABC transporter
ABC2_membrane_7 PF19055 402 - 458 8.3e-06 ABC-2 type transporter
ABC2_membrane PF01061 523 - 734 4.1e-51 ABC-2 type transporter
PDR_assoc PF08370 740 - 802 2.9e-24 Plant PDR ABC transporter associated
ABC_tran PF00005 895 - 1047 1.6e-19 ABC transporter
ABC2_membrane_7 PF19055 1076 - 1136 3.5e-07 ABC-2 type transporter
ABC2_membrane PF01061 1193 - 1406 3.1e-63 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1477, 4268
AatII GACGTC 1 cut(s) 2616
Acc36I ACCTGC 1 cut(s) 2396
AccB1I GGYRCC 1 cut(s) 2744
AccI GTMKAC 4 cut(s) 1670, 2289, 2474, 3939
AccII CGCG 5 cut(s) 1515, 1616, 3089, 3302, 3758
AclWI GGATC 7 cut(s) 359, 383, 1836, 2447, 2991, 3732, 4178
AcoI YGGCCR 3 cut(s) 1516, 2775, 3390
AcsI RAATTY 1 cut(s) 969
AcuI CTGAAG 2 cut(s) 2559, 3123
AcyI GRCGYC 2 cut(s) 1953, 2613
AdeI CACNNNGTG 1 cut(s) 3331
AfiI CCNNNNNNNGG 7 cut(s) 29, 32, 623, 1360, 2019, 3140, 3524
AflIII ACRYGT 4 cut(s) 1048, 1614, 1761, 4232
AjiI CACGTC 1 cut(s) 1668
AjnI CCWGG 4 cut(s) 586, 1847, 2635, 2710
Alw21I GWGCWC 3 cut(s) 1191, 2074, 4258
AlwI GGATC 7 cut(s) 359, 383, 1836, 2447, 2991, 3732, 4178
AoxI GGCC 4 cut(s) 1516, 2708, 2775, 3390
ApeKI GCWGC 9 cut(s) 167, 923, 1526, 1597, 1606, 1658, 2006, 3158, 3842
ApoI RAATTY 1 cut(s) 969
ArsI GACNNNNNNTTYG 2 cut(s) 622, 654
AspLEI GCGC 1 cut(s) 2728
AspS9I GGNCC 8 cut(s) 611, 1076, 1277, 1851, 3305, 4188, 4288, 4319
AsuC2I CCSGG 4 cut(s) 887, 3042, 3357, 3389
AsuII TTCGAA 1 cut(s) 3957
AsuNHI GCTAGC 1 cut(s) 3410
AvaII GGWCC 8 cut(s) 611, 1076, 1277, 1851, 3305, 4188, 4288, 4319
BaeI ACNNNNGTAYC 2 cut(s) 1441, 1474
BanI GGYRCC 1 cut(s) 2744
BbsI GAAGAC 6 cut(s) 237, 2439, 2933, 3679, 3982, 4345
Bbv12I GWGCWC 3 cut(s) 1191, 2074, 4258
BbvCI CCTCAGC 1 cut(s) 4084
BbvI GCAGC 9 cut(s) 154, 910, 1538, 1584, 1618, 1645, 1993, 3170, 3854
BceAI ACGGC 2 cut(s) 1923, 4045
BcgI CGANNNNNNTGC 2 cut(s) 1697, 1731
BciT130I CCWGG 4 cut(s) 588, 1849, 2637, 2712
BciVI GTATCC 2 cut(s) 2789, 4247
BclI TGATCA 2 cut(s) 1752, 2440
BcnI CCSGG 4 cut(s) 887, 3042, 3357, 3389
BfaI CTAG 7 cut(s) 810, 2189, 2853, 3146, 3153, 3234, 3411
BfmI CTRYAG 3 cut(s) 222, 2478, 3829
BfuAI ACCTGC 1 cut(s) 2396
BfuI GTATCC 2 cut(s) 2789, 4247
BglII AGATCT 4 cut(s) 461, 2465, 3535, 3620
BlpI GCTNAGC 3 cut(s) 333, 546, 3426
BmcAI AGTACT 3 cut(s) 1391, 1547, 3341
Bme1390I CCNGG 8 cut(s) 588, 887, 1849, 2637, 2712, 3042, 3357, 3389
Bme18I GGWCC 8 cut(s) 611, 1076, 1277, 1851, 3305, 4188, 4288, 4319
BmgBI CACGTC 1 cut(s) 1668
BmgT120I GGNCC 8 cut(s) 611, 1076, 1277, 1851, 3305, 4188, 4288, 4319
BmiI GGNNCC 5 cut(s) 612, 2326, 2746, 3306, 4320
BmrFI CCNGG 8 cut(s) 588, 887, 1849, 2637, 2712, 3042, 3357, 3389
BmrI ACTGGG 2 cut(s) 1402, 2164
BmsI GCATC 7 cut(s) 1705, 2018, 2552, 3258, 3390, 3539, 4389
BmtI GCTAGC 1 cut(s) 3414
BmuI ACTGGG 2 cut(s) 1402, 2164
BpiI GAAGAC 6 cut(s) 237, 2439, 2933, 3679, 3982, 4345
BpmI CTGGAG 3 cut(s) 1053, 1187, 3609
Bpu10I CCTNAGC 1 cut(s) 4084
Bpu1102I GCTNAGC 3 cut(s) 333, 546, 3426
Bpu14I TTCGAA 1 cut(s) 3957
BpuEI CTTGAG 4 cut(s) 912, 1316, 1556, 3864
BpuMI CCSGG 4 cut(s) 887, 3042, 3357, 3389
Bsa29I ATCGAT 1 cut(s) 378
BsaAI YACGTR 1 cut(s) 4235
BsaBI GATNNNNATC 4 cut(s) 9, 900, 1649, 1719
BsaHI GRCGYC 2 cut(s) 1953, 2613
BsaI GGTCTC 1 cut(s) 2643
BsaWI WCCGGW 1 cut(s) 1426
BsaXI ACNNNNNCTCC 6 cut(s) 27, 57, 2506, 2536, 3276, 3306
Bsc4I CCNNNNNNNGG 7 cut(s) 29, 32, 623, 1360, 2019, 3140, 3524
Bse118I RCCGGY 1 cut(s) 2747
Bse3DI GCAATG 4 cut(s) 1653, 1736, 2514, 3473
Bse8I GATNNNNATC 4 cut(s) 9, 900, 1649, 1719
BseBI CCWGG 4 cut(s) 588, 1849, 2637, 2712
BseCI ATCGAT 1 cut(s) 378
BseGI GGATG 8 cut(s) 600, 1844, 1920, 2009, 2226, 2290, 3133, 3405
BseJI GATNNNNATC 4 cut(s) 9, 900, 1649, 1719
BseLI CCNNNNNNNGG 7 cut(s) 29, 32, 623, 1360, 2019, 3140, 3524
BseMI GCAATG 4 cut(s) 1653, 1736, 2514, 3473
BseRI GAGGAG 6 cut(s) 48, 874, 1190, 2154, 2511, 3297
BseXI GCAGC 9 cut(s) 154, 910, 1538, 1584, 1618, 1645, 1993, 3170, 3854
BseYI CCCAGC 1 cut(s) 653
BsgI GTGCAG 3 cut(s) 2059, 2574, 3202
Bsh1236I CGCG 5 cut(s) 1515, 1616, 3089, 3302, 3758
BshFI GGCC 4 cut(s) 1518, 2710, 2777, 3392
BshNI GGYRCC 1 cut(s) 2744
BshVI ATCGAT 1 cut(s) 378
BsiHKAI GWGCWC 3 cut(s) 1191, 2074, 4258
BsiSI CCGG 7 cut(s) 887, 1427, 2748, 2778, 3041, 3357, 3389
BslFI GGGAC 6 cut(s) 1483, 1598, 2540, 3035, 3588, 4332
BslI CCNNNNNNNGG 7 cut(s) 29, 32, 623, 1360, 2019, 3140, 3524
BsmBI CGTCTC 1 cut(s) 203
BsmFI GGGAC 6 cut(s) 1483, 1598, 2540, 3035, 3588, 4332
BsnI GGCC 4 cut(s) 1518, 2710, 2777, 3392
Bso31I GGTCTC 1 cut(s) 2643
Bsp119I TTCGAA 1 cut(s) 3957
Bsp1286I GDGCHC 3 cut(s) 1191, 2074, 4258
Bsp1720I GCTNAGC 3 cut(s) 333, 546, 3426
Bsp19I CCATGG 1 cut(s) 3945
BspANI GGCC 4 cut(s) 1518, 2710, 2777, 3392
BspDI ATCGAT 1 cut(s) 378
BspFNI CGCG 5 cut(s) 1515, 1616, 3089, 3302, 3758
BspHI TCATGA 4 cut(s) 213, 255, 777, 909
BspLI GGNNCC 5 cut(s) 612, 2326, 2746, 3306, 4320
BspMAI CTGCAG 1 cut(s) 2482
BspMI ACCTGC 1 cut(s) 2396
BspOI GCTAGC 1 cut(s) 3414
BspPI GGATC 7 cut(s) 359, 383, 1836, 2447, 2991, 3732, 4178
BspT104I TTCGAA 1 cut(s) 3957
BspT107I GGYRCC 1 cut(s) 2744
BspTNI GGTCTC 1 cut(s) 2643
BsrDI GCAATG 4 cut(s) 1653, 1736, 2514, 3473
BsrFI RCCGGY 1 cut(s) 2747
BssAI RCCGGY 1 cut(s) 2747
BssNI GRCGYC 2 cut(s) 1953, 2613
BssT1I CCWWGG 5 cut(s) 1273, 2367, 2653, 3945, 4246
Bst2UI CCWGG 4 cut(s) 588, 1849, 2637, 2712
Bst6I CTCTTC 8 cut(s) 471, 911, 1097, 1682, 2354, 2488, 3272, 4115
BstACI GRCGYC 2 cut(s) 1953, 2613
BstAPI GCANNNNNTGC 1 cut(s) 3752
BstBAI YACGTR 1 cut(s) 4235
BstBI TTCGAA 1 cut(s) 3957
BstC8I GCNNGC 5 cut(s) 855, 1209, 1957, 2557, 3412
BstDSI CCRYGG 4 cut(s) 1182, 3060, 3210, 3945
BstEII GGTNACC 2 cut(s) 245, 704
BstENI CCTNNNNNAGG 3 cut(s) 27, 30, 1358
BstF5I GGATG 8 cut(s) 600, 1844, 1920, 2009, 2226, 2290, 3133, 3405
BstFNI CGCG 5 cut(s) 1515, 1616, 3089, 3302, 3758
BstHHI GCGC 1 cut(s) 2728
BstMWI GCNNNNNNNGC 9 cut(s) 542, 923, 1603, 1606, 1784, 1952, 2540, 3158, 3752
BstNI CCWGG 4 cut(s) 588, 1849, 2637, 2712
BstNSI RCATGY 2 cut(s) 1765, 2635
BstPI GGTNACC 2 cut(s) 245, 704
BstSCI CCNGG 8 cut(s) 586, 885, 1847, 2635, 2710, 3040, 3355, 3387
BstSFI CTRYAG 3 cut(s) 222, 2478, 3829
BstUI CGCG 5 cut(s) 1515, 1616, 3089, 3302, 3758
BstV1I GCAGC 9 cut(s) 154, 910, 1538, 1584, 1618, 1645, 1993, 3170, 3854
BstV2I GAAGAC 6 cut(s) 237, 2439, 2933, 3679, 3982, 4345
BstX2I RGATCY 9 cut(s) 351, 461, 1828, 2452, 2465, 3535, 3620, 3724, 4170
BstXI CCANNNNNNTGG 2 cut(s) 4152, 4247
BstYI RGATCY 9 cut(s) 351, 461, 1828, 2452, 2465, 3535, 3620, 3724, 4170
Bsu15I ATCGAT 1 cut(s) 378
BsuI GTATCC 2 cut(s) 2789, 4247
BsuRI GGCC 4 cut(s) 1518, 2710, 2777, 3392
BsuTUI ATCGAT 1 cut(s) 378
BtgI CCRYGG 4 cut(s) 1182, 3060, 3210, 3945
BtgZI GCGATG 1 cut(s) 4324
BtrI CACGTC 1 cut(s) 1668
BtsCI GGATG 8 cut(s) 600, 1844, 1920, 2009, 2226, 2290, 3133, 3405
BtsI GCAGTG 1 cut(s) 3816
BtsIMutI CAGTG 9 cut(s) 450, 688, 951, 1110, 3198, 3198, 3816, 3828, 4329
BveI ACCTGC 1 cut(s) 2396
Cac8I GCNNGC 5 cut(s) 855, 1209, 1957, 2557, 3412
CciI TCATGA 4 cut(s) 213, 255, 777, 909
CfoI GCGC 1 cut(s) 2728
Cfr10I RCCGGY 1 cut(s) 2747
Cfr13I GGNCC 8 cut(s) 611, 1076, 1277, 1851, 3305, 4188, 4288, 4319
ClaI ATCGAT 1 cut(s) 378
CseI GACGC 4 cut(s) 881, 1961, 2444, 3158
CspCI CAANNNNNGTGG 4 cut(s) 1099, 1134, 3072, 3107
DraIII CACNNNGTG 1 cut(s) 3331
DrdI GACNNNNNNGTC 2 cut(s) 1477, 4268
DseDI GACNNNNNNGTC 2 cut(s) 1477, 4268
EaeI YGGCCR 3 cut(s) 1516, 2775, 3390
Eam1104I CTCTTC 8 cut(s) 471, 911, 1097, 1682, 2354, 2488, 3272, 4115
EarI CTCTTC 8 cut(s) 471, 911, 1097, 1682, 2354, 2488, 3272, 4115
Eco130I CCWWGG 5 cut(s) 1273, 2367, 2653, 3945, 4246
Eco31I GGTCTC 1 cut(s) 2643
Eco32I GATATC 4 cut(s) 103, 568, 1240, 3244
Eco47I GGWCC 8 cut(s) 611, 1076, 1277, 1851, 3305, 4188, 4288, 4319
Eco57I CTGAAG 2 cut(s) 2559, 3123
Eco91I GGTNACC 2 cut(s) 245, 704
EcoNI CCTNNNNNAGG 3 cut(s) 27, 30, 1358
EcoO109I RGGNCCY 1 cut(s) 611
EcoO65I GGTNACC 2 cut(s) 245, 704
EcoRII CCWGG 4 cut(s) 586, 1847, 2635, 2710
EcoRV GATATC 4 cut(s) 103, 568, 1240, 3244
EcoT14I CCWWGG 5 cut(s) 1273, 2367, 2653, 3945, 4246
ErhI CCWWGG 5 cut(s) 1273, 2367, 2653, 3945, 4246
Esp3I CGTCTC 1 cut(s) 203
FalI AAGNNNNNCTT 8 cut(s) 141, 173, 534, 566, 1076, 1108, 1515, 1547
FaqI GGGAC 6 cut(s) 1483, 1598, 2540, 3035, 3588, 4332
FauI CCCGC 2 cut(s) 2901, 3295
FauNDI CATATG 1 cut(s) 3868
FbaI TGATCA 2 cut(s) 1752, 2440
FblI GTMKAC 4 cut(s) 1670, 2289, 2474, 3939
FokI GGATG 8 cut(s) 607, 1831, 1927, 1996, 2233, 2297, 3140, 3412
FspBI CTAG 7 cut(s) 810, 2189, 2853, 3146, 3153, 3234, 3411
GlaI GCGC 1 cut(s) 2727
GsaI CCCAGC 1 cut(s) 657
GsuI CTGGAG 3 cut(s) 1053, 1187, 3609
HaeIII GGCC 4 cut(s) 1518, 2710, 2777, 3392
HapII CCGG 7 cut(s) 887, 1427, 2748, 2778, 3041, 3357, 3389
HgaI GACGC 4 cut(s) 881, 1961, 2444, 3158
HhaI GCGC 1 cut(s) 2728
Hin1I GRCGYC 2 cut(s) 1953, 2613
Hin6I GCGC 1 cut(s) 2726
HinP1I GCGC 1 cut(s) 2726
HincII GTYRAC 3 cut(s) 139, 2629, 3445
HindII GTYRAC 3 cut(s) 139, 2629, 3445
HindIII AAGCTT 4 cut(s) 281, 915, 2255, 3434
HpaII CCGG 7 cut(s) 887, 1427, 2748, 2778, 3041, 3357, 3389
Hpy99I CGWCG 3 cut(s) 146, 1909, 2615
HpyCH4IV ACGT 6 cut(s) 1048, 1667, 2613, 2769, 3611, 4234
HpyF10VI GCNNNNNNNGC 9 cut(s) 542, 923, 1603, 1606, 1784, 1952, 2540, 3158, 3752
HpySE526I ACGT 6 cut(s) 1048, 1667, 2613, 2769, 3611, 4234
Hsp92I GRCGYC 2 cut(s) 1953, 2613
HspAI GCGC 1 cut(s) 2726
Ksp22I TGATCA 2 cut(s) 1752, 2440
Lsp1109I GCAGC 9 cut(s) 154, 910, 1538, 1584, 1618, 1645, 1993, 3170, 3854
LweI GCATC 7 cut(s) 1705, 2018, 2552, 3258, 3390, 3539, 4389
MaeI CTAG 7 cut(s) 810, 2189, 2853, 3146, 3153, 3234, 3411
MaeII ACGT 6 cut(s) 1048, 1667, 2613, 2769, 3611, 4234
MflI RGATCY 9 cut(s) 351, 461, 1828, 2452, 2465, 3535, 3620, 3724, 4170
MhlI GDGCHC 3 cut(s) 1191, 2074, 4258
MluCI AATT 5 cut(s) 530, 969, 2996, 3263, 3554
MluI ACGCGT 1 cut(s) 1614
MlyI GAGTC 6 cut(s) 512, 787, 1797, 2522, 2667, 3017
MmeI TCCRAC 6 cut(s) 425, 985, 1054, 1233, 2302, 2701
MslI CAYNNNNRTG 7 cut(s) 212, 1173, 1247, 1760, 3122, 3398, 3592
MspA1I CMGCKG 3 cut(s) 1346, 3161, 3425
MspI CCGG 7 cut(s) 887, 1427, 2748, 2778, 3041, 3357, 3389
MspR9I CCNGG 8 cut(s) 588, 887, 1849, 2637, 2712, 3042, 3357, 3389
MvaI CCWGG 4 cut(s) 588, 1849, 2637, 2712
MvnI CGCG 5 cut(s) 1515, 1616, 3089, 3302, 3758
MwoI GCNNNNNNNGC 9 cut(s) 542, 923, 1603, 1606, 1784, 1952, 2540, 3158, 3752
NciI CCSGG 4 cut(s) 887, 3042, 3357, 3389
NcoI CCATGG 1 cut(s) 3945
NdeI CATATG 1 cut(s) 3868
NheI GCTAGC 1 cut(s) 3410
NlaIV GGNNCC 5 cut(s) 612, 2326, 2746, 3306, 4320
NmuCI GTSAC 6 cut(s) 949, 1051, 1921, 2692, 3046, 3883
NspI RCATGY 2 cut(s) 1765, 2635
NspV TTCGAA 1 cut(s) 3957
PagI TCATGA 4 cut(s) 213, 255, 777, 909
PciI ACATGT 1 cut(s) 1761
PcsI WCGNNNNNNNCGW 2 cut(s) 225, 4347
PflFI GACNNNGTC 1 cut(s) 2612
PfoI TCCNGGA 2 cut(s) 885, 1847
PleI GAGTC 6 cut(s) 511, 787, 1797, 2522, 2667, 3017
PpsI GAGTC 6 cut(s) 511, 787, 1797, 2522, 2667, 3017
Ppu21I YACGTR 1 cut(s) 4235
PpuMI RGGWCCY 1 cut(s) 611
PscI ACATGT 1 cut(s) 1761
Psp5II RGGWCCY 1 cut(s) 611
Psp6I CCWGG 4 cut(s) 586, 1847, 2635, 2710
PspEI GGTNACC 2 cut(s) 245, 704
PspFI CCCAGC 1 cut(s) 653
PspGI CCWGG 4 cut(s) 586, 1847, 2635, 2710
PspN4I GGNNCC 5 cut(s) 612, 2326, 2746, 3306, 4320
PspPI GGNCC 8 cut(s) 611, 1076, 1277, 1851, 3305, 4188, 4288, 4319
PspPPI RGGWCCY 1 cut(s) 611
PsrI GAACNNNNNNTAC 4 cut(s) 1479, 1511, 3498, 3530
PstI CTGCAG 1 cut(s) 2482
PsuI RGATCY 9 cut(s) 351, 461, 1828, 2452, 2465, 3535, 3620, 3724, 4170
PsyI GACNNNGTC 1 cut(s) 2612
PvuII CAGCTG 1 cut(s) 1346
RseI CAYNNNNRTG 7 cut(s) 212, 1173, 1247, 1760, 3122, 3398, 3592
Sau96I GGNCC 8 cut(s) 611, 1076, 1277, 1851, 3305, 4188, 4288, 4319
ScaI AGTACT 3 cut(s) 1391, 1547, 3341
SchI GAGTC 6 cut(s) 512, 787, 1797, 2522, 2667, 3017
ScrFI CCNGG 8 cut(s) 588, 887, 1849, 2637, 2712, 3042, 3357, 3389
SduI GDGCHC 3 cut(s) 1191, 2074, 4258
SfaNI GCATC 7 cut(s) 1705, 2018, 2552, 3258, 3390, 3539, 4389
SfcI CTRYAG 3 cut(s) 222, 2478, 3829
SfuI TTCGAA 1 cut(s) 3957
SinI GGWCC 8 cut(s) 611, 1076, 1277, 1851, 3305, 4188, 4288, 4319
SmiMI CAYNNNNRTG 7 cut(s) 212, 1173, 1247, 1760, 3122, 3398, 3592
SmlI CTYRAG 4 cut(s) 927, 1295, 1571, 3879
SmoI CTYRAG 4 cut(s) 927, 1295, 1571, 3879
Sse9I AATT 5 cut(s) 530, 969, 2996, 3263, 3554
SspI AATATT 1 cut(s) 1896
SspMI CTAG 7 cut(s) 810, 2189, 2853, 3146, 3153, 3234, 3411
StyD4I CCNGG 8 cut(s) 586, 885, 1847, 2635, 2710, 3040, 3355, 3387
StyI CCWWGG 5 cut(s) 1273, 2367, 2653, 3945, 4246
TaiI ACGT 6 cut(s) 1051, 1670, 2616, 2772, 3614, 4237
TaqII GACCGA 2 cut(s) 1294, 2894
TasI AATT 5 cut(s) 530, 969, 2996, 3263, 3554
TatI WGTACW 6 cut(s) 1389, 1545, 3339, 3793, 3852, 4333
TauI GCSGC 7 cut(s) 1518, 1962, 2537, 3164, 3425, 3758, 4331
TscAI CASTG 9 cut(s) 457, 688, 958, 1117, 3198, 3205, 3816, 3828, 4329
TseFI GTSAC 6 cut(s) 949, 1051, 1921, 2692, 3046, 3883
TseI GCWGC 9 cut(s) 167, 923, 1526, 1597, 1606, 1658, 2006, 3158, 3842
Tsp45I GTSAC 6 cut(s) 949, 1051, 1921, 2692, 3046, 3883
TspGWI ACGGA 2 cut(s) 3077, 3632
TspRI CASTG 9 cut(s) 457, 688, 958, 1117, 3198, 3205, 3816, 3828, 4329
Tth111I GACNNNGTC 1 cut(s) 2612
VpaK11BI GGWCC 8 cut(s) 611, 1076, 1277, 1851, 3305, 4188, 4288, 4319
XagI CCTNNNNNAGG 3 cut(s) 27, 30, 1358
XapI RAATTY 1 cut(s) 969
XceI RCATGY 2 cut(s) 1765, 2635
XcmI CCANNNNNNNNNTGG 3 cut(s) 640, 3121, 4247
XmiI GTMKAC 4 cut(s) 1670, 2289, 2474, 3939
XspI CTAG 7 cut(s) 810, 2189, 2853, 3146, 3153, 3234, 3411
ZraI GACGTC 1 cut(s) 2614
ZrmI AGTACT 3 cut(s) 1391, 1547, 3341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.