MD17G1184700.v1.1
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002725
Physical Location & Seq
Reverse (-)
21808769 .. 21818639
9871 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1184700.v1.1.491

Sequence Viewer

Length: 4494 bp
ATGGAGGGAACAGAGAAAGCCAAGGGTTCACAGCCACATCGTCAAAGGCGCCATAGCCGCAGTGGAAGCATAGGCAGGAGCCTGAGCAGGGCAAGTTGGAGCATGGAAGAAGTATTTGCGAGTGCCACGCACTCGAGAAGAAGCAGCCACGTCGACGAAGACGAAGAAGCTCTCACATGGGCTGCCATCGAGAAACTGCCCACGTATGATCGGCTAAGAACAAGCATCATAAAGTCCCTCGGGGAAACTGAGCCTCAAGGAGTACATAAGCAAGTAGACGTTCTGAAGCTCGACATCAACGACCGTCAGAATTTCATTGACAGGATTTTCAAGGTTGCGGAGGAAGATAATGATAAGTTCCTGAAAAAGTTCAGAAGTAGAATTGATAAGGTTGGGATCAGACTTCCCACAGTGGAAGTAAGGTTTGAACATTTGACAGTTGAAGCTGATTGCCATATTGGAACAAGAGCTCTTCCCACCCTCCCAAATGTTGCTCGGAACATCGCAGAATCGGCTCTTGGCTTAATTGGGATTAAGTTGACTAAGCAAACCAAATTAACAATTCTGAAAGATGTTTCTGGCATTGTTAAACCTTCAAGGATGGCCCTTTTATTAGGTCCCCCATCCTCGGGTAAAACTACCCTTTTGCTGGCTTTAGCTGGGAAGTTGGATCCAAGCTTGAAGGTTAAAGGAGATGTGACCTACAATGGTCACAGGCTGAATGAGTTTGTACCACAGAAGACATCAGCGTACATTAGCCAAAACGATGTTCATGTTGGAAATATGACGGTCAAAGAAACTCTAGATTTTTCAGCAAGGTGTCAAGGGGTTGGCTCTCGATATGAACTTCTTTCTGAGCTTGCTAGAAGAGAAAAGGCCGCTGGTATATATCCAGAACCAGAAGTAGACCTCTTCATGAAGGCTACTTCAATGGGAGGAGTTGAGAGCAGTATCATTACTGATTATACTCTAAAAATTTTGGGGCTTGATATATGTAAGGACACACTTGTTGGAGATGAGATGCAAAGAGGAATATCTGGTGGTCAGAAAAAACGAGTAACCACAGGTGAGATGATTGTTGGACCAACGAAAACATTGTTCATGGATGAAATCTCAACGGGTCTCGATAGCTCCACGACATTCCAAATCGTGAAGTGCCTACAACAAATTGTACACATCACTGAGGCTACAATCTTGATGTCTCTACTGCAACCAGCTCCTGAGACATTTGATCTTTTCGATGATATCATCCTTATATCAGAAGGTCAGATTGTCTACCAAGGACCACGAGTGAATATATTACAATTCTTTGAGACATGTGGATTTCGATGCCCTGAGAGAAAGGGAACTGCTGATTTCTTGCAAGAGGTTACCTCAAGAAAAGACCAAGAGCAGTATTGGGCAGATAGAAGAACTCCATACAGATATGTATCGGTCACAGAATTTGCAAACCTGTTCAAGCGTTTCCATGTGGGCATGAGGATGGAGAATGAGCTCTCAATCCCATTCGACAAGGCTCGAGGCCACAAAGCAGCTCTTGAATTCAGAAGATATTCAATACCCAAAATGCAGCTTCTCAAAGCCTGTTTTGACAAAGAATGGTTGCTCATGCAGAGGAACTCTTTTATTTACATTTTCAAGACTGTCCAAATTATAATAGGGGCATTCATAGCCGCGACAGTGTTTTTGAAGACCGAAATGAACACTAGGAATGAAGATGATGGAGCACTCTATGTTGGTGCACTTATATTTGTCATGATAATTAACATGTTTAATGGTTTTGCTGAGCTCTCATTGACCATTTCAAGACTTCCTGTGTTTTACAAGCACAGAGACCTTCTTTTCCACCCTGCTTGGACTTTCACTCTCCCATCTGTCCTGCTTGGGATTCCTATATCCATCATGGAAACTACTATTTGGATGGCCATTACATATTACACCATTGGATTTGCACCTGAAGCTAGCAGGTTTTTCAAGCAACTATTGTTGGTATTTCTGATCCAACAAATGGCAGCTGCGCTATTTAGGCTTATAGCTGGAGTTAGCAGGACCATCATCATATCAAACACCGGCGGGTCTCTAACTGTTCTAATTGTTTTCATGCTTGGAGGTTTCATTATTCCTCATGGTCAAATTCCAAAATGGTGGGTGTGGGGATACTGGGTTTCACCTATGACATATGGCTTCAATGCAGTCGCTGTAAATGAAATGTATTCTCCGAGGTGGTCGAACAAACTGTCTTCAGATAATATTACCAGCGTGGGTGAGGCAGTGCTTAAAAACTTTGATGTTTACCGCAACAAAAACTGGTTTTGGATTGGCTCTGCAGCTCTTCTGGGGTTTGCAGTTCTCTTCAACGTGCTTTATACATTTGCCCTTATGTACCTAAATGCTCCTGGAAAGCCACAAGCCATCATATCTGAAGAAGCAGCAAACAAGATGGAGGTAGACCAAGAAGAATCAAAGGAAGAGCCAAGACTTAGAAGGCCCCCGTCCAAGAAAGATTTTCAGTCTCGATCATTATCTTCTAATGATGGAAACAACTCAAGAGAAATGGAAGTCCGGCGAATGGGCAGTCGATCCAGCTCTACTGGACTAAGTAGAAATCCTGATTCGTCTCTCGAAGTTGCCAGTGGTGTTGTCCCTAAAAGAGGAATGGTTCTACCTTTCACTCCTCTTGCAATGTCCTTCAACGACGTTAACTATTACGTGGATATGCCTGCTGAAATGAAGGAAGAAGGAGTAGCAGGGGACCGGCTGCAACTACTCCGTGAAGTAACTGGTGCATTTAGGCCTGGGGTCTTGACTGCCTTAATGGGGGTCAGTGGGGCTGGGAAGACAACTTTGATGGATGTCTTAGCAGGAAGGAAGACTGGAGGTTATATTGAAGGTGACATTAGAATTTCTGGGTACCCTAAGAAGCAAGAAACCTTTGCAAGAATTTCTGGTTATTGCGAACAAACTGATATCCACTCACCCCAAGTCACTGTCAAAGAATCATTGATCTATTCAGCTTTCCTTAGACTCCCTAAAGAAGTTAGCAACGAGGAAAAGATGATTTTTGTTGATCAAGTGATGGAATTGGTTGAGCTAGATAATCTCAAAGATGCTCTAGTAGGGCTTCCTGGAGTTTCAGGACTGTCAACAGAACAAAGAAAGAGGTTAACGATTGCAGTTGAGCTTGTTGCCAATCCCTCGATCATTTTCATGGATGAACCCACATCAGGTCTTGATGCAAGGGCAGCTGCCATTGTTATGAGGACTGTTAGAAATACTGTGGACACTGGGAGAACAGTTGTTTGCACAATTCATCAGCCTAGCATAGATATCTTTGAGGCATTTGATGAGCTGCTACTGATGAAGAGAGGAGGACAAGTTATCTACTCAGGACCACTAGGCCGCAATTCTCACAAGATTGTTGAATACTTTGAGGCAGTTCCTGGCGTGGCAAAAATCAAAGAGAAGTATAACCCAGCAACGTGGATGCTCGAGGTGAGCTCAACCTCAACTGAGGTCCGTCTTGGAATGGACTTTGCTCAATACTACAAATCAGCTGCCTTGTACCAGAGAAACAAAACGTTAGTAAAGGAGTTGAGCGTCCCACCAGCAGGAGCAAAAGACCTCTATTTTCCGACTCAGTTTTCTCAATCGTCCTGGAAGCAATTCCAATCTTGCCTCTGGAAGCAGTGGTGGACATATTGGAGAAGTCCTGATTATAACCTTGTCAGATTCTTTTTTACCTTGGCAGCTGCCCTCCTCCTTGGAACTATTTTCTGGAAGGTTGGCACTAAAAGGGAAAGCAGTTCTGATCTGACCATGATTATCGGGGCTATGTACGCTGCTGTGCTTTTCGTCGGAATTGATAACTGCGCAACTGTGCAGCCAATTGTCGCCATTGAAAGAACTGTTTTCTATCGAGAACGAGCTGCTGGGATGTACTCTGCATTACCTTATGCACTGGCACAGGTCATTGTGGAAATACCGTATGTGTTTATTCAGACCACGTATTATACACTGATAGTGTATGCTATGGTGAGCTTCCAATGGAAATTAGAAAAATTCTTCTGGTTCTTCTTTATCAACTTCTTCTCCTTCCTTTACTTCACATACTACGGCATGATGACCGTTTCCATCACGCCAAACCACCAAGTAGCAGCCATATTTGCCGCAGCTTTCTATTCAGTCTTCAACCTCTTCTCCGGTTTCTTCATTCCAAGACCAAAAATACCCAAATGGTGGGTTTGGTACTACTGGATATGTCCCGTGGCATGGACAGTGTATGGATTGATTGTGTCACAGTACGGCGATGTTGAGGAAACCATTAGAGCGCCTGGGATTACACCTAACCCAACTATAAAATGGTACATAGAAGACCATTTCGGATATGATCCAAATTTCATGGGACCAGTGGCTGGAGTTCTAGTTGGATTCACAATCTTCTTTGCCTTCATGTTTGCCTACTGTATTAGGACACTCAACTTCCAAGTTAGATAG

Protein Analysis

1498

Amino Acids

169.23

Weight (kDa)

8.8

Isoelectric Point (pI)

38.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 114 - 164 2.2e-09 ABC-transporter N-terminal
ABC_tran PF00005 189 - 370 2e-14 ABC transporter
ABC2_membrane PF01061 525 - 736 4.1e-51 ABC-2 type transporter
PDR_assoc PF08370 742 - 806 5.8e-25 Plant PDR ABC transporter associated
ABC_tran PF00005 924 - 1075 6.4e-20 ABC transporter
ABC2_membrane PF01061 1221 - 1434 7.3e-63 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1655, 3717
Acc16I TGCGCA 1 cut(s) 3871
Acc36I ACCTGC 1 cut(s) 1956
Acc65I GGTACC 1 cut(s) 2910
AccB1I GGYRCC 2 cut(s) 48, 2910
AccB7I CCANNNNNTGG 3 cut(s) 2008, 4236, 4412
AccI GTMKAC 5 cut(s) 153, 276, 906, 1275, 2448
AccII CGCG 1 cut(s) 1676
AciI CCGC 8 cut(s) 58, 338, 879, 1674, 2073, 2296, 3400, 4167
AclI AACGTT 1 cut(s) 3578
AclWI GGATC 6 cut(s) 404, 665, 678, 1993, 2574, 4382
AcoI YGGCCR 1 cut(s) 1923
AcsI RAATTY 9 cut(s) 310, 975, 1442, 1541, 2133, 2901, 2940, 4058, 4393
AcuI CTGAAG 4 cut(s) 305, 1977, 2226, 2442
AcyI GRCGYC 1 cut(s) 49
AflIII ACRYGT 2 cut(s) 1316, 1767
AjiI CACGTC 1 cut(s) 151
AjnI CCWGG 6 cut(s) 2395, 2794, 3124, 3439, 3653, 4330
AjuI GAANNNNNNNTTGG 4 cut(s) 501, 533, 753, 785
Alw21I GWGCWC 6 cut(s) 472, 1497, 1729, 1744, 1791, 3500
Alw26I GTCTC 8 cut(s) 1127, 1206, 1217, 1307, 1827, 2082, 2517, 2622
Alw44I GTGCAC 1 cut(s) 1740
AlwI GGATC 6 cut(s) 404, 665, 678, 1993, 2574, 4382
AlwNI CAGNNNCTG 4 cut(s) 1220, 2198, 3440, 4412
Ama87I CYCGRG 5 cut(s) 133, 239, 628, 1518, 3488
AoxI GGCC 7 cut(s) 603, 876, 1522, 1923, 2486, 2792, 3397
ApaLI GTGCAC 1 cut(s) 1740
ApoI RAATTY 9 cut(s) 310, 975, 1442, 1541, 2133, 2901, 2940, 4058, 4393
ArsI GACNNNNNNTTYG 6 cut(s) 1669, 1701, 2222, 2254, 2560, 2592
Asp700I GAANNNNTTC 3 cut(s) 368, 1552, 3662
Asp718I GGTACC 1 cut(s) 2910
AspLEI GCGC 4 cut(s) 51, 2020, 3872, 4330
AsuHPI GGTGA 7 cut(s) 1079, 2160, 2276, 2903, 2967, 3505, 4045
AsuNHI GCTAGC 1 cut(s) 1961
AvaI CYCGRG 5 cut(s) 133, 239, 628, 1518, 3488
AvaII GGWCC 8 cut(s) 617, 1082, 1283, 2049, 2752, 3389, 3514, 4403
BaeGI GKGCMC 1 cut(s) 1744
BalI TGGCCA 1 cut(s) 1925
BamHI GGATCC 1 cut(s) 670
BanI GGYRCC 2 cut(s) 48, 2910
BanII GRGCYC 4 cut(s) 472, 1497, 1791, 3500
BauI CACGAG 1 cut(s) 1287
BbsI GAAGAC 8 cut(s) 165, 746, 1697, 2232, 2843, 2876, 4177, 4377
Bbv12I GWGCWC 6 cut(s) 472, 1497, 1729, 1744, 1791, 3500
BceAI ACGGC 2 cut(s) 4129, 4318
BcgI CGANNNNNNTGC 2 cut(s) 133, 167
BciT130I CCWGG 6 cut(s) 2397, 2796, 3126, 3441, 3655, 4332
BciVI GTATCC 1 cut(s) 2150
BclI TGATCA 1 cut(s) 3067
BcoDI GTCTC 8 cut(s) 1127, 1206, 1217, 1307, 1827, 2082, 2517, 2622
BfaI CTAG 9 cut(s) 803, 864, 1707, 1962, 3092, 3113, 3318, 3395, 4421
BfmI CTRYAG 1 cut(s) 2325
BfoI RGCGCY 2 cut(s) 52, 4331
BfuAI ACCTGC 1 cut(s) 1956
BfuI GTATCC 1 cut(s) 2150
BlpI GCTNAGC 1 cut(s) 1785
Bme1390I CCNGG 6 cut(s) 2397, 2796, 3126, 3441, 3655, 4332
Bme18I GGWCC 8 cut(s) 617, 1082, 1283, 2049, 2752, 3389, 3514, 4403
BmeT110I CYCGRG 5 cut(s) 133, 239, 628, 1518, 3488
BmgBI CACGTC 1 cut(s) 151
BmiI GGNNCC 8 cut(s) 50, 80, 619, 672, 2489, 2753, 2912, 4404
BmrFI CCNGG 6 cut(s) 2397, 2796, 3126, 3441, 3655, 4332
BmrI ACTGGG 2 cut(s) 2170, 3294
BmsI GCATC 6 cut(s) 234, 1011, 1319, 3097, 3223, 3474
BmtI GCTAGC 1 cut(s) 1965
BmuI ACTGGG 2 cut(s) 2170, 3294
BpiI GAAGAC 8 cut(s) 165, 746, 1697, 2232, 2843, 2876, 4177, 4377
BplI GAGNNNNNCTC 4 cut(s) 1358, 1390, 3482, 3514
BpmI CTGGAG 4 cut(s) 2058, 2895, 3147, 4434
Bpu10I CCTNAGC 1 cut(s) 83
Bpu1102I GCTNAGC 1 cut(s) 1785
BpuEI CTTGAG 3 cut(s) 240, 1360, 2530
BsaAI YACGTR 3 cut(s) 204, 2710, 4005
BsaBI GATNNNNATC 2 cut(s) 1429, 2521
BsaHI GRCGYC 1 cut(s) 49
BsaI GGTCTC 3 cut(s) 1127, 1827, 2082
BsaWI WCCGGW 1 cut(s) 4199
BsaXI ACNNNNNCTCC 8 cut(s) 3360, 3390, 4073, 4103, 4181, 4211, 4407, 4437
Bse118I RCCGGY 2 cut(s) 2069, 2754
Bse3DI GCAATG 1 cut(s) 2688
Bse8I GATNNNNATC 2 cut(s) 1429, 2521
BseBI CCWGG 6 cut(s) 2397, 2796, 3126, 3441, 3655, 4332
BseJI GATNNNNATC 2 cut(s) 1429, 2521
BseMI GCAATG 1 cut(s) 2688
BseRI GAGGAG 4 cut(s) 951, 2664, 3381, 3746
BseSI GKGCMC 1 cut(s) 1744
BseYI CCCAGC 4 cut(s) 659, 2831, 3472, 3929
BsgI GTGCAG 1 cut(s) 3899
Bsh1236I CGCG 1 cut(s) 1676
Bsh1285I CGRYCG 1 cut(s) 304
BshFI GGCC 7 cut(s) 605, 878, 1524, 1925, 2488, 2794, 3399
BshNI GGYRCC 2 cut(s) 48, 2910
BsiEI CGRYCG 1 cut(s) 304
BsiHKAI GWGCWC 6 cut(s) 472, 1497, 1729, 1744, 1791, 3500
BsiHKCI CYCGRG 5 cut(s) 133, 239, 628, 1518, 3488
BsiSI CCGG 4 cut(s) 2070, 2563, 2755, 4200
BslFI GGGAC 7 cut(s) 220, 603, 2627, 2765, 3584, 4245, 4416
BsmAI GTCTC 8 cut(s) 1127, 1206, 1217, 1307, 1827, 2082, 2517, 2622
BsmBI CGTCTC 1 cut(s) 2622
BsmFI GGGAC 7 cut(s) 220, 603, 2627, 2765, 3584, 4245, 4416
BsmI GAATGC 1 cut(s) 1664
BsnI GGCC 7 cut(s) 605, 878, 1524, 1925, 2488, 2794, 3399
Bso31I GGTCTC 3 cut(s) 1127, 1827, 2082
BsoBI CYCGRG 5 cut(s) 133, 239, 628, 1518, 3488
Bsp1286I GDGCHC 6 cut(s) 472, 1497, 1729, 1744, 1791, 3500
Bsp1407I TGTACA 1 cut(s) 1171
Bsp1720I GCTNAGC 1 cut(s) 1785
BspACI CCGC 8 cut(s) 58, 338, 879, 1674, 2073, 2296, 3400, 4167
BspANI GGCC 7 cut(s) 605, 878, 1524, 1925, 2488, 2794, 3399
BspFNI CGCG 1 cut(s) 1676
BspHI TCATGA 2 cut(s) 915, 1755
BspLI GGNNCC 8 cut(s) 50, 80, 619, 672, 2489, 2753, 2912, 4404
BspMAI CTGCAG 1 cut(s) 2329
BspMI ACCTGC 1 cut(s) 1956
BspOI GCTAGC 1 cut(s) 1965
BspPI GGATC 6 cut(s) 404, 665, 678, 1993, 2574, 4382
BspQI GCTCTTC 3 cut(s) 477, 2337, 2463
BspT107I GGYRCC 2 cut(s) 48, 2910
BspTNI GGTCTC 3 cut(s) 1127, 1827, 2082
BsrDI GCAATG 1 cut(s) 2688
BsrFI RCCGGY 2 cut(s) 2069, 2754
BsrGI TGTACA 1 cut(s) 1171
BssAI RCCGGY 2 cut(s) 2069, 2754
BssNI GRCGYC 1 cut(s) 49
BssSI CACGAG 1 cut(s) 1287
BssT1I CCWWGG 4 cut(s) 21, 1279, 3741, 3760
Bst2BI CACGAG 1 cut(s) 1287
Bst2UI CCWGG 6 cut(s) 2397, 2796, 3126, 3441, 3655, 4332
Bst6I CTCTTC 8 cut(s) 477, 862, 917, 2337, 2357, 2463, 3356, 4199
BstACI GRCGYC 1 cut(s) 49
BstAUI TGTACA 1 cut(s) 1171
BstBAI YACGTR 3 cut(s) 204, 2710, 4005
BstC8I GCNNGC 4 cut(s) 651, 861, 1963, 2721
BstDSI CCRYGG 1 cut(s) 4263
BstEII GGTNACC 1 cut(s) 1369
BstENI CCTNNNNNAGG 1 cut(s) 2649
BstFNI CGCG 1 cut(s) 1676
BstH2I RGCGCY 2 cut(s) 52, 4331
BstHHI GCGC 4 cut(s) 51, 2020, 3872, 4330
BstMAI GTCTC 8 cut(s) 1127, 1206, 1217, 1307, 1827, 2082, 2517, 2622
BstMCI CGRYCG 1 cut(s) 304
BstMWI GCNNNNNNNGC 9 cut(s) 57, 66, 512, 1670, 1958, 2026, 3242, 3836, 4163
BstNI CCWGG 6 cut(s) 2397, 2796, 3126, 3441, 3655, 4332
BstNSI RCATGY 2 cut(s) 1320, 1771
BstPI GGTNACC 1 cut(s) 1369
BstSCI CCNGG 6 cut(s) 2395, 2794, 3124, 3439, 3653, 4330
BstSFI CTRYAG 1 cut(s) 2325
BstSLI GKGCMC 1 cut(s) 1744
BstUI CGCG 1 cut(s) 1676
BstV2I GAAGAC 8 cut(s) 165, 746, 1697, 2232, 2843, 2876, 4177, 4377
BstX2I RGATCY 1 cut(s) 670
BstXI CCANNNNNNTGG 2 cut(s) 2145, 3480
BstYI RGATCY 1 cut(s) 670
BsuI GTATCC 1 cut(s) 2150
BsuRI GGCC 7 cut(s) 605, 878, 1524, 1925, 2488, 2794, 3399
BtgI CCRYGG 1 cut(s) 4263
BtgZI GCGATG 2 cut(s) 487, 4320
BtrI CACGTC 1 cut(s) 151
BtsI GCAGTG 3 cut(s) 67, 2277, 3692
BveI ACCTGC 1 cut(s) 1956
Cac8I GCNNGC 4 cut(s) 651, 861, 1963, 2721
CaiI CAGNNNCTG 4 cut(s) 1220, 2198, 3440, 4412
CciI TCATGA 2 cut(s) 915, 1755
CfoI GCGC 4 cut(s) 51, 2020, 3872, 4330
Cfr10I RCCGGY 2 cut(s) 2069, 2754
CseI GACGC 1 cut(s) 3586
CspCI CAANNNNNGTGG 2 cut(s) 1835, 1870
DinI GGCGCC 1 cut(s) 50
EaeI YGGCCR 1 cut(s) 1923
Eam1104I CTCTTC 8 cut(s) 477, 862, 917, 2337, 2357, 2463, 3356, 4199
EarI CTCTTC 8 cut(s) 477, 862, 917, 2337, 2357, 2463, 3356, 4199
Ecl136II GAGCTC 4 cut(s) 470, 1495, 1789, 3498
Eco130I CCWWGG 4 cut(s) 21, 1279, 3741, 3760
Eco147I AGGCCT 1 cut(s) 2794
Eco24I GRGCYC 4 cut(s) 472, 1497, 1791, 3500
Eco31I GGTCTC 3 cut(s) 1127, 1827, 2082
Eco32I GATATC 3 cut(s) 1246, 2968, 3328
Eco47I GGWCC 8 cut(s) 617, 1082, 1283, 2049, 2752, 3389, 3514, 4403
Eco53kI GAGCTC 4 cut(s) 470, 1495, 1789, 3498
Eco57I CTGAAG 4 cut(s) 305, 1977, 2226, 2442
Eco88I CYCGRG 5 cut(s) 133, 239, 628, 1518, 3488
Eco91I GGTNACC 1 cut(s) 1369
EcoICRI GAGCTC 4 cut(s) 470, 1495, 1789, 3498
EcoNI CCTNNNNNAGG 1 cut(s) 2649
EcoO109I RGGNCCY 2 cut(s) 617, 2487
EcoO65I GGTNACC 1 cut(s) 1369
EcoRI GAATTC 1 cut(s) 1541
EcoRII CCWGG 6 cut(s) 2395, 2794, 3124, 3439, 3653, 4330
EcoRV GATATC 3 cut(s) 1246, 2968, 3328
EcoT14I CCWWGG 4 cut(s) 21, 1279, 3741, 3760
EcoT38I GRGCYC 4 cut(s) 472, 1497, 1791, 3500
EgeI GGCGCC 1 cut(s) 50
EheI GGCGCC 1 cut(s) 50
ErhI CCWWGG 4 cut(s) 21, 1279, 3741, 3760
Esp3I CGTCTC 1 cut(s) 2622
FalI AAGNNNNNCTT 2 cut(s) 1521, 1553
FaqI GGGAC 7 cut(s) 220, 603, 2627, 2765, 3584, 4245, 4416
FauI CCCGC 1 cut(s) 2066
FauNDI CATATG 1 cut(s) 2179
FbaI TGATCA 1 cut(s) 3067
FblI GTMKAC 5 cut(s) 153, 276, 906, 1275, 2448
FriOI GRGCYC 4 cut(s) 472, 1497, 1791, 3500
FspBI CTAG 9 cut(s) 803, 864, 1707, 1962, 3092, 3113, 3318, 3395, 4421
FspI TGCGCA 1 cut(s) 3871
GlaI GCGC 4 cut(s) 50, 2019, 3871, 4329
GsaI CCCAGC 4 cut(s) 663, 2835, 3476, 3933
GsuI CTGGAG 4 cut(s) 2058, 2895, 3147, 4434
HaeII RGCGCY 2 cut(s) 52, 4331
HaeIII GGCC 7 cut(s) 605, 878, 1524, 1925, 2488, 2794, 3399
HapII CCGG 4 cut(s) 2070, 2563, 2755, 4200
HgaI GACGC 1 cut(s) 3586
HhaI GCGC 4 cut(s) 51, 2020, 3872, 4330
Hin1I GRCGYC 1 cut(s) 49
Hin6I GCGC 4 cut(s) 49, 2018, 3870, 4328
HinP1I GCGC 4 cut(s) 49, 2018, 3870, 4328
HincII GTYRAC 5 cut(s) 154, 540, 2701, 3144, 3165
HindII GTYRAC 5 cut(s) 154, 540, 2701, 3144, 3165
HindIII AAGCTT 1 cut(s) 676
HinfI GANTC 9 cut(s) 509, 1888, 2459, 2612, 2996, 3024, 3634, 3729, 4428
HpaI GTTAAC 2 cut(s) 2701, 3165
HpaII CCGG 4 cut(s) 2070, 2563, 2755, 4200
HphI GGTGA 7 cut(s) 1079, 2160, 2276, 2903, 2967, 3505, 4045
Hpy99I CGWCG 4 cut(s) 155, 158, 2699, 3857
HpyCH4IV ACGT 9 cut(s) 150, 203, 279, 2358, 2697, 2709, 3479, 3578, 4004
HpyF10VI GCNNNNNNNGC 9 cut(s) 57, 66, 512, 1670, 1958, 2026, 3242, 3836, 4163
HpySE526I ACGT 9 cut(s) 150, 203, 279, 2358, 2697, 2709, 3479, 3578, 4004
Hsp92I GRCGYC 1 cut(s) 49
HspAI GCGC 4 cut(s) 49, 2018, 3870, 4328
KasI GGCGCC 1 cut(s) 48
KpnI GGTACC 1 cut(s) 2914
Ksp22I TGATCA 1 cut(s) 3067
KspAI GTTAAC 2 cut(s) 2701, 3165
LguI GCTCTTC 3 cut(s) 477, 2337, 2463
LmnI GCTCC 7 cut(s) 78, 99, 1136, 1222, 1724, 2398, 3611
LweI GCATC 6 cut(s) 234, 1011, 1319, 3097, 3223, 3474
MaeI CTAG 9 cut(s) 803, 864, 1707, 1962, 3092, 3113, 3318, 3395, 4421
MaeII ACGT 9 cut(s) 150, 203, 279, 2358, 2697, 2709, 3479, 3578, 4004
MaeIII GTNAC 9 cut(s) 697, 710, 1057, 1369, 1435, 2776, 2891, 2983, 4293
MfeI CAATTG 1 cut(s) 3885
MflI RGATCY 1 cut(s) 670
MhlI GDGCHC 6 cut(s) 472, 1497, 1729, 1744, 1791, 3500
MlsI TGGCCA 1 cut(s) 1925
MluNI TGGCCA 1 cut(s) 1925
Mly113I GGCGCC 1 cut(s) 49
MlyI GAGTC 2 cut(s) 3018, 3628
MmeI TCCRAC 9 cut(s) 77, 648, 757, 991, 1060, 2026, 3656, 3835, 4405
Mox20I TGGCCA 1 cut(s) 1925
MroXI GAANNNNTTC 3 cut(s) 368, 1552, 3662
MscI TGGCCA 1 cut(s) 1925
MslI CAYNNNNRTG 3 cut(s) 1481, 3206, 3254
Msp20I TGGCCA 1 cut(s) 1925
MspA1I CMGCKG 5 cut(s) 881, 2015, 3245, 3554, 3749
MspI CCGG 4 cut(s) 2070, 2563, 2755, 4200
MspR9I CCNGG 6 cut(s) 2397, 2796, 3126, 3441, 3655, 4332
MunI CAATTG 1 cut(s) 3885
Mva1269I GAATGC 1 cut(s) 1664
MvaI CCWGG 6 cut(s) 2397, 2796, 3126, 3441, 3655, 4332
MvnI CGCG 1 cut(s) 1676
MwoI GCNNNNNNNGC 9 cut(s) 57, 66, 512, 1670, 1958, 2026, 3242, 3836, 4163
NarI GGCGCC 1 cut(s) 49
NdeI CATATG 1 cut(s) 2179
NheI GCTAGC 1 cut(s) 1961
NlaIV GGNNCC 8 cut(s) 50, 80, 619, 672, 2489, 2753, 2912, 4404
NmuCI GTSAC 6 cut(s) 697, 710, 1435, 2891, 2983, 4293
NsbI TGCGCA 1 cut(s) 3871
NspI RCATGY 2 cut(s) 1320, 1771
PaeR7I CTCGAG 3 cut(s) 133, 1518, 3488
PagI TCATGA 2 cut(s) 915, 1755
PceI AGGCCT 1 cut(s) 2794
PciI ACATGT 2 cut(s) 1316, 1767
PciSI GCTCTTC 3 cut(s) 477, 2337, 2463
PcsI WCGNNNNNNNCGW 2 cut(s) 159, 297
PctI GAATGC 1 cut(s) 1664
PdmI GAANNNNTTC 3 cut(s) 368, 1552, 3662
PfeI GAWTC 7 cut(s) 509, 1888, 2459, 2612, 2996, 3729, 4428
PflMI CCANNNNNTGG 3 cut(s) 2008, 4236, 4412
PfoI TCCNGGA 3 cut(s) 2395, 3124, 3653
PleI GAGTC 2 cut(s) 3018, 3628
PluTI GGCGCC 1 cut(s) 52
PpsI GAGTC 2 cut(s) 3018, 3628
Ppu21I YACGTR 3 cut(s) 204, 2710, 4005
PpuMI RGGWCCY 1 cut(s) 617
PscI ACATGT 2 cut(s) 1316, 1767
PsiI TTATAA 2 cut(s) 1655, 3717
Psp124BI GAGCTC 4 cut(s) 472, 1497, 1791, 3500
Psp1406I AACGTT 1 cut(s) 3578
Psp5II RGGWCCY 1 cut(s) 617
Psp6I CCWGG 6 cut(s) 2395, 2794, 3124, 3439, 3653, 4330
PspEI GGTNACC 1 cut(s) 1369
PspFI CCCAGC 4 cut(s) 659, 2831, 3472, 3929
PspGI CCWGG 6 cut(s) 2395, 2794, 3124, 3439, 3653, 4330
PspN4I GGNNCC 8 cut(s) 50, 80, 619, 672, 2489, 2753, 2912, 4404
PspPPI RGGWCCY 1 cut(s) 617
PspXI VCTCGAGB 2 cut(s) 1518, 3488
PstI CTGCAG 1 cut(s) 2329
PstNI CAGNNNCTG 4 cut(s) 1220, 2198, 3440, 4412
PsuI RGATCY 1 cut(s) 670
PvuII CAGCTG 4 cut(s) 2015, 3245, 3554, 3749
RseI CAYNNNNRTG 3 cut(s) 1481, 3206, 3254
SacI GAGCTC 4 cut(s) 472, 1497, 1791, 3500
SalI GTCGAC 1 cut(s) 152
SapI GCTCTTC 3 cut(s) 477, 2337, 2463
SchI GAGTC 2 cut(s) 3018, 3628
ScrFI CCNGG 6 cut(s) 2397, 2796, 3126, 3441, 3655, 4332
SduI GDGCHC 6 cut(s) 472, 1497, 1729, 1744, 1791, 3500
SfaNI GCATC 6 cut(s) 234, 1011, 1319, 3097, 3223, 3474
SfcI CTRYAG 1 cut(s) 2325
SfoI GGCGCC 1 cut(s) 50
Sfr274I CTCGAG 3 cut(s) 133, 1518, 3488
SgrAI CRCCGGYG 1 cut(s) 2069
SgrDI CGTCGACG 1 cut(s) 152
SinI GGWCC 8 cut(s) 617, 1082, 1283, 2049, 2752, 3389, 3514, 4403
SlaI CTCGAG 3 cut(s) 133, 1518, 3488
SmiMI CAYNNNNRTG 3 cut(s) 1481, 3206, 3254
SmlI CTYRAG 6 cut(s) 133, 255, 1375, 1518, 2545, 3488
SmoI CTYRAG 6 cut(s) 133, 255, 1375, 1518, 2545, 3488
SseBI AGGCCT 1 cut(s) 2794
SsiI CCGC 8 cut(s) 58, 338, 879, 1674, 2073, 2296, 3400, 4167
SspDI GGCGCC 1 cut(s) 48
SspI AATATT 1 cut(s) 2251
SspMI CTAG 9 cut(s) 803, 864, 1707, 1962, 3092, 3113, 3318, 3395, 4421
SstI GAGCTC 4 cut(s) 472, 1497, 1791, 3500
StuI AGGCCT 1 cut(s) 2794
StyD4I CCNGG 6 cut(s) 2395, 2794, 3124, 3439, 3653, 4330
StyI CCWWGG 4 cut(s) 21, 1279, 3741, 3760
TaiI ACGT 9 cut(s) 153, 206, 282, 2361, 2700, 2712, 3482, 3581, 4007
TaqII GACCGA 2 cut(s) 1423, 1709
TatI WGTACW 3 cut(s) 262, 1171, 3936
TauI GCSGC 5 cut(s) 60, 881, 1676, 3402, 4169
TfiI GAWTC 7 cut(s) 509, 1888, 2459, 2612, 2996, 3729, 4428
TseFI GTSAC 6 cut(s) 697, 710, 1435, 2891, 2983, 4293
Tsp45I GTSAC 6 cut(s) 697, 710, 1435, 2891, 2983, 4293
TspGWI ACGGA 2 cut(s) 2759, 3506
Van91I CCANNNNNTGG 3 cut(s) 2008, 4236, 4412
VneI GTGCAC 1 cut(s) 1740
VpaK11BI GGWCC 8 cut(s) 617, 1082, 1283, 2049, 2752, 3389, 3514, 4403
XagI CCTNNNNNAGG 1 cut(s) 2649
XapI RAATTY 9 cut(s) 310, 975, 1442, 1541, 2133, 2901, 2940, 4058, 4393
XbaI TCTAGA 1 cut(s) 802
XceI RCATGY 2 cut(s) 1320, 1771
XcmI CCANNNNNNNNNTGG 2 cut(s) 59, 4356
XhoI CTCGAG 3 cut(s) 133, 1518, 3488
XmiI GTMKAC 5 cut(s) 153, 276, 906, 1275, 2448
XmnI GAANNNNTTC 3 cut(s) 368, 1552, 3662
XspI CTAG 9 cut(s) 803, 864, 1707, 1962, 3092, 3113, 3318, 3395, 4421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.