MD09G1204300.v1.1
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002725
Physical Location & Seq
Reverse (-)
19342328 .. 19352785
10458 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1204300.v1.1.491

Sequence Viewer

Length: 4494 bp
ATGGAGGGAACAGAGAAACCGAAAGGTTCAGAGCCACATCATCAAAAGCGCCATAGCCGCAGCGGAAGCATAAGCAGGAGCCTGAGCAGGGCAAGTTGGCGCATGGAAGAAGTATTTGCGACTGCCACGCACTCGAGAAGAAGCAGCCAAGCGGACGAAGACGAAGAAGCTCTGACATGGGCTGCCATTGAGAAACTGCCCACGTATGATCGGCTAAGAACAAGCATCATCAAGTCCCTTGGGGAAACTGAGCATCAAGGAGTACATAAGCAAGTAGACGTTCTACAGCTCGACATGGACGACCGTCAGAATTTCATTCAAAGGGTTTTCAAGGTTGCAGAGGAAGATAATGAGACGTTCCTGAAAAAGTTCAGAAGTAGACTTGATAAGGTTGGGATCAAACTTCCCACAGTGGAAGTAAGGTACGAGCATTTGACAATTGAAGCTGATTGCCATGTTGGAACAAGAGCTCTTCCCACCCTCCCAAATGTTGCTCGGAACATCGCAGAATCAGCTCTTGGCTTAATTGGGATTAAGTTGACTAAGCAAACAAAACTAACAATTCTCAAAGATGTCTCTGGCATTATTAAACCTTCAAGGATGGCCCTTTTATTAGGTCCTCCATCCTCTGGGAAGACTTCCCTTTTACTGGCTTTAGCTGGGAAGTTGGATTCAAGCTTGAAGGTTAAGGGAGAAATAACTTACAATGGTCACCGGCTGAATGAGTTTGTGCCACGGAAGACATCTGCATATATTAGCCAAAATGATCTTCATGTTGGAAATATGACGGTCAAAGAAACACTAGATTTTTCAGCAAGGTGCCAAGGGGTTGGGACACGATATGAACTTCTTACTGAGCTTGCTAGAAGAGAAAAGGCCGCTGGAATATATCCAGAGCCCGAAGTAGATCTTTTCATGAAGGCTACTGCAATGGGAGGACTTGAGAGCAGTCTCATTACTGATTATACTCTCAAAATTTTGGGGCTTGATATATGTAAGGACACAATTGTTGGAGATGAGATGCAGAGAGGGATATCTGGTGGCCAGAAAAAACGAGTAACCACAGGTGAGATGATTGTTGGACCTACTAAAACATTGTTCATGGATGAGATCTCAACGGGTCTGGATAGCTCCACGACGTTCCAAATCGTGAAGTGCCTACAACAAATTGTACACATCACTGAGGCTACGATCTTGATGTCTTTACTCCAACCTGCTCCTGAGACATTTGATCTTTTTGATGATATCATCCTTTTATCAGAAGGCCAGATCGTCTACCAGGGACCACGTGAGCACATATTAGAGTTCTTTGAGACATTTGGATTTCGATGCCCTGAGAGAAAGGGAACTGCCGATTTCTTGCAAGAGGTTACCTCAAGAAAAGACCAGGAGCAGTATTGGGCAGACAGAAGGACTCCATACAGATATGTATCGGTCACAGAATTTGCAAACCGGTTCAAGCGTTTCCATGTGGGCACGAAGATAGCGGATGAGCTCTCAATCCCATTCGACAAGGCTCAAAGCCACAAAGCAGCTCTTGTATTCAAAACATATTCAATGCCCAAAATGCAGCTTCTCAAAGCATGTTTTGACAAAGAATGGCTGCTCATGCAGAGGAACTCATTTATTTACATTTTCAAGACTGTCCAAATTATAATAGGGGCAATCATAGCGGGGACAGTGTTTTTGAGGACCGAAATGGACACTAGAAATGAAAATGATGGATCACTCTATGTTGGTGCGCTTATATATTCCCTAATCATTAATATGTTTAATGGGTTTGCTGAGCTCTCATTGACCATTGCAAGACTTCCTGTGTTTTACAAGCATAGAGACCTTCTTTTCCACCCTGCTTGGACTTTCACTCTCCCATCTGTCCTGCTTGGGATTCCTATATCCATCATAGAATCCACTGTTTTTATAGGCATTACATACTACACCATTGGATTTGCACCTGAAGCTAGCAGGTTTTTCAAGCAACTATTGTTGATATTTCTGACGCAACAAATGGCATCTGCGCTATTTAGGCTTATTGCTGGAGTTAGCAGGACCATGATCATATCCAACACTGGCGGGACTCTTACTCTTCTAATTCTTTTCTTGCTTGGAGGTTTCATAATTCCAAGAGGTCAAATTCCAAACTGGTGGACGTGGGCATACTGGGTTTCACCTTTGACATATGGCTTCAATGCTATCTCTGTAAATGAAATGTATGCTCCGAGGTGGATGAACAAACTGGCTTTAGATAATGTTACCAGGGTGGGTGAGGCAGTGCTTAATAACTTTGATGTTTACCACAACAAAAACTGGTTTTGGATTGGTTCTGGCGCTCTTCTGGGGTTTATTGTTCTCTTCAACTCCCTTTATACGGTCTCCCTTATGTACCTAAGTGCTCCTGGAAAGCCACAAGCCATCATATCTGAAGAGGCGGCAAATGAAATGGAGCTAGACCAAGAAGAATCAAAGGAAGAGCCAAGACTGAGAAGACCCCCATCCAAGAATGTTTCTTTGCCTCGATCATTATCTTCTACTGATGGAAACAATACAAGAGAAATGGAAGTCCGACGAATGAGCAGTCGATCCAGCTCTAGTGGACTAGATAGAAATGCTGATTCGTCTCTCGAAGTCTCTGGTGGCGTTGCCCCCAAGAGAGGAATGGTTTTACCTTTCACACCTCTTGCAATGTCCTTCGACAGTGTTAATTATTTCGTGGACATGCCCGCTGAAATGAAGGAAGAAGGAGTAGCAGAGGACAGGCTGCAACTACTTCGTGAAGTAACTGGTGCATTTAGGCCTGGGGTCTTGACTGCCCTAATGGGGGTAAGTGGAGCTGGGAAGACAACTTTGATGGATGTCTTAGCAGGAAGAAAGACCGGAGGTTACATTGAAGGTGACATTAGAATTTCTGGGTACCCTAAGAAGCAAGAAACCTTTGCAAGAATTTCTGGTTACTGTGAACAAACTGATATCCACTCACCCCAAGTCACTATCAAAGAATCGTTGGTTTACTCAGCTTTCCTTAGACTCCCTAAAGAAGTCAGCAACGAGGAAAAGATGATTTTTGTAGATCAAGTGATGGAACTGGTTGAGCTGGACAATCTTAAAGATGCTTTAGTAGGGCTTCCCGGAGTTTCAGGGCTGTCAACAGAACAAAGAAAGAGGTTAACGATTGCAGTTGAGCTTGTTGCCAATCCCTCAATCATTTTCATGGATGAACCAACATCAGGTCTTGATGCAAGGGCAGCTGCCATCGTTATGAGGACTGTTAGAAATACGGTGGACACTGGGAGAACAGTTGTTTGCACAATTCATCAGCCTAGCATAGATATCTTTGAGGCATTCGATGAGCTACTACTGATGAAGAGAGGAGGACAAGTTATCTACTTAGGACCACTGGGCCGCAATTCTCACAAGATTGTTGAATATTTTGAGGCGGTTCCTGGCGTGCCAAAAATCAAGGAGAAGTACAATCCAGCTACATGGATGCTTGAGGCGAGCTCAACATCAATTGAGGTCCGGCTTGGAATGGACTTTGCTCAATTCTACAAATCATCTGCCTTGCATCAGAGAAACAAAGCTTTAGTAAATGAGTTGAGCACACCACCAGCAGGAGCAAACGACCTCTATTTTCCCACTCAGTTTTCTCAGCCGTCATGGAAGCAATTCCAGTCTTGCCTCTGGAAGCAGTGGTGGACTTATTGGAGAAGTCCTGATTATAACCTTGTCAGATTCTTTTTTACCTTGGCAGCAGCCCTTCTCGTTGGATCTATTTTCTGGGATATTGGCTCAAAAATCGAAAGCAGTTCTGATATGAACATGGTTATCGGGGCTATGTACGCTGCTGTCTTTTTCGTCGGATTTGATAACTGCACAACGGTGCAACCCATTGTCGCCATTGAAAGAACAGTTTTCTATCGAGAAAGAGCTGCTGGGATGTACTCTGCATTACCTTATGCACTGGCACAGGTCATTATTGAAATACCATATGTGTTTATCCAGACCACGTATTATACACTGATAGTGTATGCTATGGTGAGCTTCCAATGGGAAGCAGGAAAATTCTTCTGGTTCTTCTTTATCAACTTCTTCTCCTTCCTTTACTTCACATACTACGGCATGATGACCGTTTCCATCACACCGAACCACCAAGTAGCAGCCATATTCGCCGCAGCTTTCTATTCAGTCTTCAATCTCTTCTCCGGTTTCTTCATTCCAAGACCAAAAATACCCGGTTGGTGGATTTGGTACTACTGGATATGTCCCGTGGCATGGACGGTGTATGGATTGATTGTAACACAGTATGGTGACATTGAGGAGACCATTAAAGCACCTGGGTATACACCTGACCCAACTGTGAAATGGTACCTAAAAGACCGTTACGGATTTGAATCAGATTTCAAGGGACCAGTGGCTGGAGTTCTAGTTGGATTCGGAGTCTTCTTCGCCTTCATGTTTGCCTTTTGCATTAGGAAACTCAACTTCCAAGTTAGATAG

Protein Analysis

1498

Amino Acids

169.05

Weight (kDa)

7.55

Isoelectric Point (pI)

38.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 114 - 164 4.5e-09 ABC-transporter N-terminal
ABC_tran PF00005 189 - 370 1.4e-13 ABC transporter
ABC2_membrane PF01061 525 - 736 1.3e-53 ABC-2 type transporter
PDR_assoc PF08370 742 - 806 8.7e-23 Plant PDR ABC transporter associated
ABC_tran PF00005 924 - 1075 1.1e-19 ABC transporter
ABC2_membrane PF01061 1221 - 1434 1.1e-62 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1655, 3717
Acc36I ACCTGC 2 cut(s) 1222, 1956
Acc65I GGTACC 2 cut(s) 2910, 4362
AccB1I GGYRCC 3 cut(s) 819, 2910, 4362
AccB7I CCANNNNNTGG 2 cut(s) 629, 4412
AccI GTMKAC 4 cut(s) 276, 379, 1275, 4337
AclWI GGATC 4 cut(s) 404, 1732, 2574, 3772
AcoI YGGCCR 1 cut(s) 1042
AcsI RAATTY 7 cut(s) 310, 975, 1442, 2133, 2901, 2940, 4058
AcuI CTGAAG 2 cut(s) 1977, 2442
AcvI CACGTG 1 cut(s) 1289
AgeI ACCGGT 1 cut(s) 1452
AjiI CACGTC 1 cut(s) 2151
AjnI CCWGG 7 cut(s) 1278, 1386, 2255, 2395, 2794, 3439, 4330
AjuI GAANNNNNNNTTGG 4 cut(s) 501, 533, 753, 785
AleI CACNNNNGTG 1 cut(s) 3875
Alw21I GWGCWC 7 cut(s) 472, 1296, 1497, 1791, 2395, 3500, 3599
AlwI GGATC 4 cut(s) 404, 1732, 2574, 3772
AlwNI CAGNNNCTG 1 cut(s) 4412
Ama87I CYCGRG 1 cut(s) 133
AoxI GGCC 6 cut(s) 603, 876, 1042, 1264, 2792, 3397
ApoI RAATTY 7 cut(s) 310, 975, 1442, 2133, 2901, 2940, 4058
ArsI GACNNNNNNTTYG 4 cut(s) 1669, 1701, 2560, 2592
AseI ATTAAT 1 cut(s) 1764
AsiGI ACCGGT 1 cut(s) 1452
Asp700I GAANNNNTTC 2 cut(s) 368, 3662
Asp718I GGTACC 2 cut(s) 2910, 4362
AspLEI GCGC 5 cut(s) 51, 102, 1744, 2020, 2330
AsuC2I CCSGG 2 cut(s) 3126, 4230
AsuHPI GGTGA 8 cut(s) 704, 1079, 2160, 2276, 2903, 2967, 4045, 4316
AsuNHI GCTAGC 1 cut(s) 1961
AvaI CYCGRG 1 cut(s) 133
AvaII GGWCC 8 cut(s) 617, 1082, 1283, 1692, 2049, 3389, 3514, 4403
BaeGI GKGCMC 1 cut(s) 1478
BalI TGGCCA 1 cut(s) 1044
BanI GGYRCC 3 cut(s) 819, 2910, 4362
BanII GRGCYC 5 cut(s) 472, 900, 1497, 1791, 3500
BbrPI CACGTG 1 cut(s) 1289
BbsI GAAGAC 7 cut(s) 165, 641, 746, 2491, 2843, 4177, 4429
Bbv12I GWGCWC 7 cut(s) 472, 1296, 1497, 1791, 2395, 3500, 3599
BceAI ACGGC 2 cut(s) 3634, 4129
BcgI CGANNNNNNTGC 2 cut(s) 2750, 2784
BciT130I CCWGG 7 cut(s) 1280, 1388, 2257, 2397, 2796, 3441, 4332
BclI TGATCA 1 cut(s) 2055
BcnI CCSGG 2 cut(s) 3126, 4230
BfaI CTAG 9 cut(s) 803, 864, 1707, 1962, 2447, 2589, 2597, 3318, 4421
BfmI CTRYAG 1 cut(s) 284
BfoI RGCGCY 2 cut(s) 52, 2331
BfuAI ACCTGC 2 cut(s) 1222, 1956
BglII AGATCT 2 cut(s) 907, 1110
BlpI GCTNAGC 1 cut(s) 1785
Bme1390I CCNGG 9 cut(s) 1280, 1388, 2257, 2397, 2796, 3126, 3441, 4230, 4332
Bme18I GGWCC 8 cut(s) 617, 1082, 1283, 1692, 2049, 3389, 3514, 4403
BmeT110I CYCGRG 1 cut(s) 133
BmgBI CACGTC 1 cut(s) 2151
BmiI GGNNCC 7 cut(s) 80, 821, 1284, 2912, 3438, 4364, 4404
BmrFI CCNGG 9 cut(s) 1280, 1388, 2257, 2397, 2796, 3126, 3441, 4230, 4332
BmrI ACTGGG 3 cut(s) 2170, 3294, 3404
BmsI GCATC 9 cut(s) 234, 262, 1011, 1319, 2021, 3097, 3223, 3474, 3571
BmtI GCTAGC 1 cut(s) 1965
BmuI ACTGGG 3 cut(s) 2170, 3294, 3404
BoxI GACNNNNGTC 1 cut(s) 303
BpiI GAAGAC 7 cut(s) 165, 641, 746, 2491, 2843, 4177, 4429
BplI GAGNNNNNCTC 4 cut(s) 1358, 1390, 3482, 3514
BpmI CTGGAG 2 cut(s) 2058, 4434
Bpu10I CCTNAGC 1 cut(s) 83
Bpu1102I GCTNAGC 1 cut(s) 1785
BpuEI CTTGAG 3 cut(s) 962, 1360, 3509
BpuMI CCSGG 2 cut(s) 3126, 4230
BsaAI YACGTR 3 cut(s) 204, 1289, 4005
BsaBI GATNNNNATC 3 cut(s) 1429, 2521, 4387
BsaI GGTCTC 3 cut(s) 1827, 2377, 4310
BsaWI WCCGGW 3 cut(s) 1452, 2873, 4199
BsaXI ACNNNNNCTCC 6 cut(s) 3360, 3390, 4073, 4103, 4407, 4437
Bse118I RCCGGY 2 cut(s) 714, 1452
Bse3DI GCAATG 3 cut(s) 936, 1800, 2688
Bse8I GATNNNNATC 3 cut(s) 1429, 2521, 4387
BseBI CCWGG 7 cut(s) 1280, 1388, 2257, 2397, 2796, 3441, 4332
BseJI GATNNNNATC 3 cut(s) 1429, 2521, 4387
BseMI GCAATG 3 cut(s) 936, 1800, 2688
BseRI GAGGAG 2 cut(s) 3381, 4328
BseSI GKGCMC 1 cut(s) 1478
BseYI CCCAGC 3 cut(s) 659, 2831, 3929
BsgI GTGCAG 1 cut(s) 3853
Bsh1285I CGRYCG 1 cut(s) 304
BshFI GGCC 6 cut(s) 605, 878, 1044, 1266, 2794, 3399
BshNI GGYRCC 3 cut(s) 819, 2910, 4362
BshTI ACCGGT 1 cut(s) 1452
BsiEI CGRYCG 1 cut(s) 304
BsiHKAI GWGCWC 7 cut(s) 472, 1296, 1497, 1791, 2395, 3500, 3599
BsiHKCI CYCGRG 1 cut(s) 133
BsiSI CCGG 7 cut(s) 715, 1453, 2874, 3126, 3517, 4200, 4230
BslFI GGGAC 7 cut(s) 220, 847, 1296, 1690, 2089, 4245, 4416
BsmBI CGTCTC 2 cut(s) 347, 2622
BsmFI GGGAC 7 cut(s) 220, 847, 1296, 1690, 2089, 4245, 4416
BsmI GAATGC 1 cut(s) 3338
BsnI GGCC 6 cut(s) 605, 878, 1044, 1266, 2794, 3399
Bso31I GGTCTC 3 cut(s) 1827, 2377, 4310
BsoBI CYCGRG 1 cut(s) 133
Bsp1286I GDGCHC 9 cut(s) 472, 900, 1296, 1478, 1497, 1791, 2395, 3500, 3599
Bsp1407I TGTACA 1 cut(s) 1171
Bsp1720I GCTNAGC 1 cut(s) 1785
BspANI GGCC 6 cut(s) 605, 878, 1044, 1266, 2794, 3399
BspHI TCATGA 1 cut(s) 915
BspLI GGNNCC 7 cut(s) 80, 821, 1284, 2912, 3438, 4364, 4404
BspMI ACCTGC 2 cut(s) 1222, 1956
BspOI GCTAGC 1 cut(s) 1965
BspPI GGATC 4 cut(s) 404, 1732, 2574, 3772
BspQI GCTCTTC 3 cut(s) 477, 2337, 2463
BspT107I GGYRCC 3 cut(s) 819, 2910, 4362
BspTNI GGTCTC 3 cut(s) 1827, 2377, 4310
BsrDI GCAATG 3 cut(s) 936, 1800, 2688
BsrFI RCCGGY 2 cut(s) 714, 1452
BsrGI TGTACA 1 cut(s) 1171
BssAI RCCGGY 2 cut(s) 714, 1452
BssNAI GTATAC 1 cut(s) 4338
BssT1I CCWWGG 3 cut(s) 238, 823, 3741
Bst1107I GTATAC 1 cut(s) 4338
Bst2UI CCWGG 7 cut(s) 1280, 1388, 2257, 2397, 2796, 3441, 4332
Bst6I CTCTTC 9 cut(s) 477, 862, 2091, 2337, 2357, 2418, 2463, 3356, 4199
BstAUI TGTACA 1 cut(s) 1171
BstBAI YACGTR 3 cut(s) 204, 1289, 4005
BstC8I GCNNGC 5 cut(s) 861, 1963, 2721, 3446, 3496
BstDSI CCRYGG 2 cut(s) 734, 4263
BstEII GGTNACC 2 cut(s) 710, 1369
BstH2I RGCGCY 2 cut(s) 52, 2331
BstHHI GCGC 5 cut(s) 51, 102, 1744, 2020, 2330
BstMCI CGRYCG 1 cut(s) 304
BstNI CCWGG 7 cut(s) 1280, 1388, 2257, 2397, 2796, 3441, 4332
BstNSI RCATGY 2 cut(s) 1587, 2719
BstPAI GACNNNNGTC 1 cut(s) 303
BstPI GGTNACC 2 cut(s) 710, 1369
BstSCI CCNGG 9 cut(s) 1278, 1386, 2255, 2395, 2794, 3124, 3439, 4228, 4330
BstSFI CTRYAG 1 cut(s) 284
BstSLI GKGCMC 1 cut(s) 1478
BstV2I GAAGAC 7 cut(s) 165, 641, 746, 2491, 2843, 4177, 4429
BstX2I RGATCY 3 cut(s) 907, 1110, 3764
BstXI CCANNNNNNTGG 3 cut(s) 830, 2145, 3480
BstYI RGATCY 3 cut(s) 907, 1110, 3764
BstZ17I GTATAC 1 cut(s) 4338
BsuRI GGCC 6 cut(s) 605, 878, 1044, 1266, 2794, 3399
BtgI CCRYGG 2 cut(s) 734, 4263
BtgZI GCGATG 1 cut(s) 487
BtrI CACGTC 1 cut(s) 2151
BtsI GCAGTG 2 cut(s) 2277, 3692
BveI ACCTGC 2 cut(s) 1222, 1956
Cac8I GCNNGC 5 cut(s) 861, 1963, 2721, 3446, 3496
CaiI CAGNNNCTG 1 cut(s) 4412
CciI TCATGA 1 cut(s) 915
CfoI GCGC 5 cut(s) 51, 102, 1744, 2020, 2330
Cfr10I RCCGGY 2 cut(s) 714, 1452
CseI GACGC 1 cut(s) 2008
CspAI ACCGGT 1 cut(s) 1452
CspCI CAANNNNNGTGG 2 cut(s) 1835, 1870
EaeI YGGCCR 1 cut(s) 1042
Eam1104I CTCTTC 9 cut(s) 477, 862, 2091, 2337, 2357, 2418, 2463, 3356, 4199
EarI CTCTTC 9 cut(s) 477, 862, 2091, 2337, 2357, 2418, 2463, 3356, 4199
Ecl136II GAGCTC 4 cut(s) 470, 1495, 1789, 3498
Eco130I CCWWGG 3 cut(s) 238, 823, 3741
Eco147I AGGCCT 1 cut(s) 2794
Eco24I GRGCYC 5 cut(s) 472, 900, 1497, 1791, 3500
Eco31I GGTCTC 3 cut(s) 1827, 2377, 4310
Eco32I GATATC 4 cut(s) 1035, 1246, 2968, 3328
Eco47I GGWCC 8 cut(s) 617, 1082, 1283, 1692, 2049, 3389, 3514, 4403
Eco53kI GAGCTC 4 cut(s) 470, 1495, 1789, 3498
Eco57I CTGAAG 2 cut(s) 1977, 2442
Eco72I CACGTG 1 cut(s) 1289
Eco88I CYCGRG 1 cut(s) 133
Eco91I GGTNACC 2 cut(s) 710, 1369
EcoICRI GAGCTC 4 cut(s) 470, 1495, 1789, 3498
EcoO109I RGGNCCY 1 cut(s) 617
EcoO65I GGTNACC 2 cut(s) 710, 1369
EcoRII CCWGG 7 cut(s) 1278, 1386, 2255, 2395, 2794, 3439, 4330
EcoRV GATATC 4 cut(s) 1035, 1246, 2968, 3328
EcoT14I CCWWGG 3 cut(s) 238, 823, 3741
EcoT38I GRGCYC 5 cut(s) 472, 900, 1497, 1791, 3500
ErhI CCWWGG 3 cut(s) 238, 823, 3741
Esp3I CGTCTC 2 cut(s) 347, 2622
FalI AAGNNNNNCTT 4 cut(s) 894, 926, 1521, 1553
FaqI GGGAC 7 cut(s) 220, 847, 1296, 1690, 2089, 4245, 4416
FauI CCCGC 3 cut(s) 1666, 2066, 2728
FauNDI CATATG 2 cut(s) 2179, 3985
FbaI TGATCA 1 cut(s) 2055
FblI GTMKAC 4 cut(s) 276, 379, 1275, 4337
FriOI GRGCYC 5 cut(s) 472, 900, 1497, 1791, 3500
FspBI CTAG 9 cut(s) 803, 864, 1707, 1962, 2447, 2589, 2597, 3318, 4421
GlaI GCGC 5 cut(s) 50, 101, 1743, 2019, 2329
GsaI CCCAGC 3 cut(s) 663, 2835, 3933
GsuI CTGGAG 2 cut(s) 2058, 4434
HaeII RGCGCY 2 cut(s) 52, 2331
HaeIII GGCC 6 cut(s) 605, 878, 1044, 1266, 2794, 3399
HapII CCGG 7 cut(s) 715, 1453, 2874, 3126, 3517, 4200, 4230
HgaI GACGC 1 cut(s) 2008
HhaI GCGC 5 cut(s) 51, 102, 1744, 2020, 2330
Hin6I GCGC 5 cut(s) 49, 100, 1742, 2018, 2328
HinP1I GCGC 5 cut(s) 49, 100, 1742, 2018, 2328
HincII GTYRAC 3 cut(s) 540, 3144, 3165
HindII GTYRAC 3 cut(s) 540, 3144, 3165
HindIII AAGCTT 2 cut(s) 676, 3576
HpaI GTTAAC 1 cut(s) 3165
HpaII CCGG 7 cut(s) 715, 1453, 2874, 3126, 3517, 4200, 4230
HphI GGTGA 8 cut(s) 704, 1079, 2160, 2276, 2903, 2967, 4045, 4316
Hpy99I CGWCG 3 cut(s) 1141, 2568, 3857
HpyCH4IV ACGT 7 cut(s) 203, 279, 356, 1139, 1288, 2150, 4004
HpySE526I ACGT 7 cut(s) 203, 279, 356, 1139, 1288, 2150, 4004
HspAI GCGC 5 cut(s) 49, 100, 1742, 2018, 2328
KpnI GGTACC 2 cut(s) 2914, 4366
Ksp22I TGATCA 1 cut(s) 2055
KspAI GTTAAC 1 cut(s) 3165
LguI GCTCTTC 3 cut(s) 477, 2337, 2463
LmnI GCTCC 9 cut(s) 78, 1136, 1222, 1390, 2221, 2398, 2443, 2828, 3611
LweI GCATC 9 cut(s) 234, 262, 1011, 1319, 2021, 3097, 3223, 3474, 3571
MaeI CTAG 9 cut(s) 803, 864, 1707, 1962, 2447, 2589, 2597, 3318, 4421
MaeII ACGT 7 cut(s) 203, 279, 356, 1139, 1288, 2150, 4004
MfeI CAATTG 3 cut(s) 438, 1005, 3507
MflI RGATCY 3 cut(s) 907, 1110, 3764
MhlI GDGCHC 9 cut(s) 472, 900, 1296, 1478, 1497, 1791, 2395, 3500, 3599
MlsI TGGCCA 1 cut(s) 1044
MluNI TGGCCA 1 cut(s) 1044
MlyI GAGTC 4 cut(s) 1408, 2071, 3018, 4443
Mox20I TGGCCA 1 cut(s) 1044
MroXI GAANNNNTTC 2 cut(s) 368, 3662
MscI TGGCCA 1 cut(s) 1044
MslI CAYNNNNRTG 4 cut(s) 1766, 3206, 3254, 3875
Msp20I TGGCCA 1 cut(s) 1044
MspA1I CMGCKG 4 cut(s) 63, 881, 2723, 3245
MspI CCGG 7 cut(s) 715, 1453, 2874, 3126, 3517, 4200, 4230
MspR9I CCNGG 9 cut(s) 1280, 1388, 2257, 2397, 2796, 3126, 3441, 4230, 4332
MunI CAATTG 3 cut(s) 438, 1005, 3507
Mva1269I GAATGC 1 cut(s) 3338
MvaI CCWGG 7 cut(s) 1280, 1388, 2257, 2397, 2796, 3441, 4332
NciI CCSGG 2 cut(s) 3126, 4230
NdeI CATATG 2 cut(s) 2179, 3985
NheI GCTAGC 1 cut(s) 1961
NlaIV GGNNCC 7 cut(s) 80, 821, 1284, 2912, 3438, 4364, 4404
NmuCI GTSAC 5 cut(s) 710, 1435, 2891, 2983, 4304
NspI RCATGY 2 cut(s) 1587, 2719
OliI CACNNNNGTG 1 cut(s) 3875
PaeR7I CTCGAG 1 cut(s) 133
PagI TCATGA 1 cut(s) 915
PceI AGGCCT 1 cut(s) 2794
PciSI GCTCTTC 3 cut(s) 477, 2337, 2463
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PctI GAATGC 1 cut(s) 3338
PdmI GAANNNNTTC 2 cut(s) 368, 3662
PflMI CCANNNNNTGG 2 cut(s) 629, 4412
PfoI TCCNGGA 2 cut(s) 2395, 3124
PinAI ACCGGT 1 cut(s) 1452
PleI GAGTC 4 cut(s) 1408, 2071, 3018, 4442
PmaCI CACGTG 1 cut(s) 1289
PmlI CACGTG 1 cut(s) 1289
PpsI GAGTC 4 cut(s) 1408, 2071, 3018, 4442
Ppu21I YACGTR 3 cut(s) 204, 1289, 4005
PpuMI RGGWCCY 1 cut(s) 617
PshAI GACNNNNGTC 1 cut(s) 303
PshBI ATTAAT 1 cut(s) 1764
PsiI TTATAA 2 cut(s) 1655, 3717
Psp124BI GAGCTC 4 cut(s) 472, 1497, 1791, 3500
Psp5II RGGWCCY 1 cut(s) 617
Psp6I CCWGG 7 cut(s) 1278, 1386, 2255, 2395, 2794, 3439, 4330
PspCI CACGTG 1 cut(s) 1289
PspEI GGTNACC 2 cut(s) 710, 1369
PspFI CCCAGC 3 cut(s) 659, 2831, 3929
PspGI CCWGG 7 cut(s) 1278, 1386, 2255, 2395, 2794, 3439, 4330
PspN4I GGNNCC 7 cut(s) 80, 821, 1284, 2912, 3438, 4364, 4404
PspPPI RGGWCCY 1 cut(s) 617
PstNI CAGNNNCTG 1 cut(s) 4412
PsuI RGATCY 3 cut(s) 907, 1110, 3764
PvuII CAGCTG 1 cut(s) 3245
RseI CAYNNNNRTG 4 cut(s) 1766, 3206, 3254, 3875
SacI GAGCTC 4 cut(s) 472, 1497, 1791, 3500
SapI GCTCTTC 3 cut(s) 477, 2337, 2463
SchI GAGTC 4 cut(s) 1408, 2071, 3018, 4443
ScrFI CCNGG 9 cut(s) 1280, 1388, 2257, 2397, 2796, 3126, 3441, 4230, 4332
SduI GDGCHC 9 cut(s) 472, 900, 1296, 1478, 1497, 1791, 2395, 3500, 3599
SfaNI GCATC 9 cut(s) 234, 262, 1011, 1319, 2021, 3097, 3223, 3474, 3571
SfcI CTRYAG 1 cut(s) 284
Sfr274I CTCGAG 1 cut(s) 133
SinI GGWCC 8 cut(s) 617, 1082, 1283, 1692, 2049, 3389, 3514, 4403
SlaI CTCGAG 1 cut(s) 133
SmiMI CAYNNNNRTG 4 cut(s) 1766, 3206, 3254, 3875
SmlI CTYRAG 4 cut(s) 133, 941, 1375, 3488
SmoI CTYRAG 4 cut(s) 133, 941, 1375, 3488
SseBI AGGCCT 1 cut(s) 2794
SspI AATATT 1 cut(s) 3425
SspMI CTAG 9 cut(s) 803, 864, 1707, 1962, 2447, 2589, 2597, 3318, 4421
SstI GAGCTC 4 cut(s) 472, 1497, 1791, 3500
StuI AGGCCT 1 cut(s) 2794
StyD4I CCNGG 9 cut(s) 1278, 1386, 2255, 2395, 2794, 3124, 3439, 4228, 4330
StyI CCWWGG 3 cut(s) 238, 823, 3741
TaiI ACGT 7 cut(s) 206, 282, 359, 1142, 1291, 2153, 4007
TaqII GACCGA 2 cut(s) 1423, 1709
TatI WGTACW 4 cut(s) 262, 1171, 3465, 3936
TauI GCSGC 5 cut(s) 60, 881, 2432, 3402, 4169
TseFI GTSAC 5 cut(s) 710, 1435, 2891, 2983, 4304
Tsp45I GTSAC 5 cut(s) 710, 1435, 2891, 2983, 4304
TspGWI ACGGA 2 cut(s) 751, 4395
Van91I CCANNNNNTGG 2 cut(s) 629, 4412
VpaK11BI GGWCC 8 cut(s) 617, 1082, 1283, 1692, 2049, 3389, 3514, 4403
VspI ATTAAT 1 cut(s) 1764
XapI RAATTY 7 cut(s) 310, 975, 1442, 2133, 2901, 2940, 4058
XceI RCATGY 2 cut(s) 1587, 2719
XcmI CCANNNNNNNNNTGG 2 cut(s) 2653, 4356
XhoI CTCGAG 1 cut(s) 133
XmiI GTMKAC 4 cut(s) 276, 379, 1275, 4337
XmnI GAANNNNTTC 2 cut(s) 368, 3662
XspI CTAG 9 cut(s) 803, 864, 1707, 1962, 2447, 2589, 2597, 3318, 4421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.