Rroxscaffold_2G00104910
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002725
Physical Location & Seq
Reverse (-)
28257666 .. 28268209
10544 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00104910.1

Sequence Viewer

Length: 4362 bp
ATGGAGGGAACAGAGAGAGTAAAAGCAGGTACAAATCGAAGAAGCAGAAGCAGAACTCCGAGTTGGAGCATGGAGGAAGTGTTTGTGAGTGCCACGCACTCTCGAAGAAGCAGTAGAGCTGAAGACGAAGAGGCTCTGACTTGGGCTGCCATTGAGAAACTGCCAACATATGATAGACTAAGAACAGGGATCATCCAGTCCATTGTGGAGAGCGAGAATCCTCGAAGGAAAACCAACAAAGTAATGCACAAGGAAGTTGATGTTCTGAAGCTTGATGTAACCGACCGTCAAGATTTCATTGACAGGATTTTCAAGGATGCAGAGGAAGATAATGAGAAGTTCTTGAAGAAGTTCAGAAGCAGAATTGATAAGGTTGGGATTAGACTTCCCACAGTGGAAGTTAGGTTTGATCATGTGACGGTCGAAGCAGAATGTCATATTGGTAACCGAGCTGTTCCCACTCTCCCAAATGTTGCTAGGAACATTGCAGAATCAGCTCTTGGTTTGATTGGGATTACAACGGCTAAGAGAACAAATCTAACAATCCTCAAGAATGCCACTGGCATTATCAAACCATCGAGGATGGCTCTATTATTAGGTCCCCCATCGTCAGGGAAAACAACCCTTTTGTTGGCACTAGCTGGGAAGTTGGATCGTAGCTTGAAGGTTAAAGGAGAGATCACATACAATGGGTATAGGTTGAACGAATTTGTACCACAGAAGACATCTGCATATATTAGCCAAAATGATGTTCATTTAGGAGAGATGACTGTCAAAGAAACCCTAGATTTTTCGGCAAGGTGCCAAGGGGTTGGTACTCGATATGAACTTCTGTCTGAGCTAGCTAGAAGGGAAAAGGAAGCTGGAATATTTCCCGAGGCCGAAGTTGACCTTTTCATGAAGGCTACCGCAATGGGAGGAGTTGAGAGCAGTCTCATTACCGACTACACTCTCAAAATTTTAGGGCTTGATATATGCAAGGATACCATTGTTGGAAATGAAATGCAGAGAGGGATTTCTGGTGGCCAAAAGAAACGAGTAACGACAGGTGAGATGATTGTGGGGCCTACAAAAGCACTATTCATGGACGAAATATCAACCGGTCTGGATAGCTCCACCACATTTCAAATAGTGAAGTGCTTGCAGCAAATCGTCCACATCACTGAAGCTACGATCTTTATGTCGCTACTCCAACCTGCTCCTGAGACGTTCGATCTCTTCGATGATATCATCCTTTTATCGGAGGGTCAAATTGTCTACCAGGGACCGCGTGAGAACGTAGTAGAGTTCTTTGAGAGCTGTGGATTTCGATGCCCCGAGCGAAAAGGAACTGCTGATTTCTTGCAAGAGGTTACCTCTAGAAAAGACCAAGAGCAATATTGGGCGGATAGAAGCAAGCCATACAGATACATATCAGTGACTGAATTCTCAAACCGATTTAAACGCTTCCATGTAGGCATGAGGCTTGAAAACGAGCTCTCAATCCCTTTTGATAAGTCACAAGGCCACAAAGCAGCTCTAGTGTTGACAAGATATTCAGTATCCAAAATGGAACTTCTAAAGGCCTGTTGGGACAAGGAATGGCTACTCATAAAGAGGAACTCTTTTGTCTACATTTTTAAGACCGTCCAAATAATTATAGGGGCGTTGATAACCTCGACTGTGTTTTTGAAGACCCAAATGCACACCAGAAATGAAGATGATGGAGCAGTATATCTTGGTGCACTATTATTTACCATGATCGTTAACACTTTCAATGGTTTTGCTGAGCTCTCGATGACCATTGCAAGACTACCTGTATTTTACAAGCATAGAGACCTTCTTTTCCACCCTGCTTGGACTTTCACTCTCCCAACTATCCTGCTCACTATTCCTATATCCATTGTTGAATCCACAGTTTGGATGGTCATAACATATTACACCATTGGTTTTGCACCTGAAGCTAGCAGGTTTTTCAAGCAACTATTGTTGATATTTCTGATCCAACAAATGGCTGCTGGGATCTTCAGGCTTATTGCTGGAGTCTGCAGGACCATGATCATAGCAAACACTGGTGGGGCTCTCACTTTGCTCATGGTTTTCATGCTTGGAGGTTTCATACTACCTAAAGGCGAAATTCCAAAGTGGTGGCAGTGGGGTTACTGGGTTTCACCTATGACCTATGGTTTCAATGCTATTGCTGTAAATGAAATGTTTTCTCCCAGGTGGATGAACAAATGGGCTTCAGATAATGATACCAGATTGGGTGTGGCAGTGCTTCAAAACTTTGAAGTTTTCCCTGACAAAAACTGGTTTTGGATTGGCTCTGCAGCTCTTCTGGGGTTCGCAATTCTCTTCAACATCCTCTACACTATAGCTCTCATGTACCTCAGTCCTCCTGGAAAAGCACAAGCTATTATATCTGAAGAATTGGCCGAAGAGATGGAGGGAGACCAAGAAGAATCCAGGGAAGAACCAAGACTTAGAAGGCCCAAGTCAAAGCAAGATTCATTTTCTCAGTCATTATCATCTGCTGATGCAAACAATTCAAGTAAGAAGCAAAGCAATGCTCTCTTGTTTTTAATTGTGAACACAAGTGTTCTTATTGGACCTAAAAATCTTTCAGGAGAAATGGCAATCCGGCGTATGAGCAGTCAATCCAATACCAGTGGACTAAGTAGGAATGCTGATTCTTCCCTAGAAGTAGCCAGTGGGGTTGCGCCCAAGAGAGGAATGGTTCTTCCTTTCACTCCTCTGGCAATGTCCTTTGATGATGTTAATTACTATGTGGACATGCCTCCTGAAATGAAAGAAGAAGGAGTGACTGAGGATAGGCTGCAGCTACTTCGTGAAGTAACCGGGGCTTTTAGGCCTGGGGTCTTGACTGCTCTAATGGGGATCAGCGGGGCTGGAAAGACAACTTTGATGGATGTCTTAGCAGGAAGAAAGACTGGTGGTTACATTGAAGGCGATATTAGAATTTCCGGGTTCCCTAAGAAACAAGAAACCTTTGCAAGAATTTCCGGTTATTGTGAACAAACTGATATCCACTCACCCCAAGTCACTGTCAAAGAGTCGCTAATTTACTCAGCTTTCCTTAGGCTCCCTAAAGAAGTCAGCAAACAGGACAAGATGATTTTCGTCCAAGAGGTGATGGAATTGGTCGAGCTAGACAGTCTCAAGGATGCTCTAGTCGGGCTTCCTGGAATAACAGGGTTGTCAACCGAACAAAGAAAGAGGTTAACAATTGCAGTTGAGCTTGTTGCTAATCCCTCAATCATTTTCATGGATGAACCAACATCAGGTCTTGATGCAAGGGCAGCTGCAATTGTTATGAGGACTGTTAGAAATACGGTGGACACTGGGAGAACAGTTGTCTGCACTATTCACCAGCCTAGCATTGATATATTCGAGGCATTTGATGAGCTTCTACTCCTGAAGAGAGGAGGACAAGTTATCTACTCGGGACCATTAGGCCGCAATTCCCACAAGATCATCGAATATTTTGAGGCTATTCCTGGAGTGCAAAAAATAAAAGAAAAGTATAATCCAGCCACATGGATGCTTGAGGTAAGCTCAGTAGGAACTGAGGTCAAGCTTGGAATGGACTTTGCTCAATACTACAGATCATCTTCCTTGCACCAGAGAAACAAAGCTTTAGTAAAGGAGCTAAGCAAACCACCACCGGGAGAAAAAGATCTCTATTTTGCTACTCAGTATTCACAGTCATCATTACAACAATTCAAATCATGTCTGTGGAAGCAGTGGTGGACTTATTGGAGAACTCCTGATTATAACCTTGTTAGATTCTTTTTCACCTTGGCTTGTGCTCTCATGCTTGGAACTATGTTCTGGAAGGTTGGCACTAAAAGGGACAGCACTTCTGATCTGACCATGATTATTGGGGCTATGTATTCTGCTGTGCTGTTCGTTGGAATTAACAACTGCTCCACAGTGCAGCCAATAATCGCCACGGAAAGAACGGTATTCTATCGAGAAAGAGCTGCTGGGATGTACTCTGCATTACCTTATGCACTAGCACAGGTTATTATTGAAATGCCATATGTGTTTCTTCAGACCACATATTATACGCTGATAGTGTATGCCATGAAAATACCGAAATGGTGGGTTTGGTATTACTGGATATGCCCAGTAGCATGGACAGTGTATGGATTGATTGTATCACAATATGGTGATATTTTGGACACCATTAAAGCGCCTGGGATGACACCTGATCCAACTGTGAAATGGTATGTAGAACACCATTTTGGATACGATCCAAACTTCATGGGACCAGTGGCTGGAGTTCTGGTTGGCTTCACACTCTTCTTTGCTTTCATGTATGCTTACTGCATAAAAACTCTCAACTTCCAAATCAGGTAG

Protein Analysis

1453

Amino Acids

164.08

Weight (kDa)

8.52

Isoelectric Point (pI)

41.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 107 - 158 2.8e-08 ABC-transporter N-terminal
ABC_tran PF00005 183 - 364 4.4e-13 ABC transporter
ABC2_membrane_7 PF19055 398 - 449 5e-06 ABC-2 type transporter
ABC2_membrane PF01061 519 - 730 3.1e-54 ABC-2 type transporter
PDR_assoc PF08370 736 - 800 1.6e-26 Plant PDR ABC transporter associated
ABC_tran PF00005 943 - 1094 3e-19 ABC transporter
ABC2_membrane_7 PF19055 1123 - 1184 4.8e-06 ABC-2 type transporter
ABC2_membrane PF01061 1239 - 1363 1.2e-32 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3774
Acc36I ACCTGC 3 cut(s) 17, 1204, 1938
AccB1I GGYRCC 1 cut(s) 801
AccB7I CCANNNNNTGG 3 cut(s) 1899, 1990, 4280
AccI GTMKAC 2 cut(s) 1257, 1611
AccII CGCG 1 cut(s) 1270
AciI CCGC 5 cut(s) 909, 1268, 1385, 2883, 3457
AclWI GGATC 7 cut(s) 197, 660, 1975, 2009, 2885, 4208, 4250
AcoI YGGCCR 2 cut(s) 1024, 2412
AcsI RAATTY 6 cut(s) 707, 957, 1424, 2115, 2958, 2997
AcuI CTGAAG 9 cut(s) 141, 287, 1185, 1959, 1990, 2208, 2424, 3437, 4037
AfaI GTAC 5 cut(s) 31, 714, 817, 2366, 3995
AfiI CCNNNNNNNGG 9 cut(s) 611, 631, 1240, 1899, 1990, 2708, 3281, 3665, 4280
AgeI ACCGGT 1 cut(s) 1100
AjnI CCWGG 8 cut(s) 1260, 2201, 2377, 2444, 2851, 3181, 3496, 4198
Alw21I GWGCWC 4 cut(s) 1479, 1726, 1773, 3811
Alw26I GTCTC 5 cut(s) 938, 1199, 1809, 2424, 3161
Alw44I GTGCAC 1 cut(s) 1722
AlwI GGATC 7 cut(s) 197, 660, 1975, 2009, 2885, 4208, 4250
AlwNI CAGNNNCTG 2 cut(s) 1421, 4280
Ama87I CYCGRG 3 cut(s) 875, 1316, 3442
AoxI GGCC 9 cut(s) 879, 1024, 1064, 1504, 1563, 2412, 2468, 2849, 3454
ApaLI GTGCAC 1 cut(s) 1722
ApoI RAATTY 6 cut(s) 707, 957, 1424, 2115, 2958, 2997
ArsI GACNNNNNNTTYG 2 cut(s) 1651, 1683
AsiGI ACCGGT 1 cut(s) 1100
Asp700I GAANNNNTTC 1 cut(s) 350
AspLEI GCGC 2 cut(s) 2701, 4198
AspS9I GGNCC 8 cut(s) 599, 1064, 1265, 2031, 2469, 2588, 3446, 4271
AsuC2I CCSGG 3 cut(s) 2839, 2965, 3666
AsuHPI GGTGA 7 cut(s) 1061, 2142, 3024, 3142, 3359, 3787, 4184
AsuNHI GCTAGC 2 cut(s) 841, 1943
AvaI CYCGRG 3 cut(s) 875, 1316, 3442
AvaII GGWCC 6 cut(s) 599, 1265, 2031, 2588, 3446, 4271
AxyI CCTNAGG 1 cut(s) 3077
BaeGI GKGCMC 1 cut(s) 1726
BaeI ACNNNNGTAYC 2 cut(s) 975, 1008
BalI TGGCCA 1 cut(s) 1026
BanI GGYRCC 1 cut(s) 801
BanII GRGCYC 3 cut(s) 1479, 1773, 2062
BbsI GAAGAC 3 cut(s) 129, 728, 1679
Bbv12I GWGCWC 4 cut(s) 1479, 1726, 1773, 3811
BccI CCATC 8 cut(s) 577, 583, 613, 1697, 1897, 2416, 2899, 3127
BceAI ACGGC 1 cut(s) 537
BcgI CGANNNNNNTGC 2 cut(s) 2807, 2841
BciT130I CCWGG 8 cut(s) 1262, 2203, 2379, 2446, 2853, 3183, 3498, 4200
BciVI GTATCC 3 cut(s) 976, 1552, 4244
BclI TGATCA 2 cut(s) 409, 2037
BcnI CCSGG 3 cut(s) 2839, 2965, 3666
BcoDI GTCTC 5 cut(s) 938, 1199, 1809, 2424, 3161
BfmI CTRYAG 5 cut(s) 2026, 2307, 2352, 2816, 3601
BfoI RGCGCY 1 cut(s) 4199
BfuAI ACCTGC 3 cut(s) 17, 1204, 1938
BfuI GTATCC 3 cut(s) 976, 1552, 4244
BglII AGATCT 1 cut(s) 3676
BlpI GCTNAGC 2 cut(s) 1767, 3650
Bme18I GGWCC 6 cut(s) 599, 1265, 2031, 2588, 3446, 4271
BmeT110I CYCGRG 3 cut(s) 875, 1316, 3442
BmgT120I GGNCC 8 cut(s) 599, 1064, 1265, 2031, 2469, 2588, 3446, 4271
BmiI GGNNCC 8 cut(s) 601, 803, 1065, 1266, 2969, 3083, 3447, 4272
BmrI ACTGGG 3 cut(s) 2152, 3351, 4124
BmsI GCATC 6 cut(s) 307, 1301, 2506, 3154, 3280, 3531
BmtI GCTAGC 2 cut(s) 845, 1947
BmuI ACTGGG 3 cut(s) 2152, 3351, 4124
BpiI GAAGAC 3 cut(s) 129, 728, 1679
BplI GAGNNNNNCTC 6 cut(s) 571, 603, 1340, 1372, 3539, 3571
BpmI CTGGAG 3 cut(s) 2040, 3519, 4302
Bpu1102I GCTNAGC 2 cut(s) 1767, 3650
BpuEI CTTGAG 3 cut(s) 533, 3143, 3566
BpuMI CCSGG 3 cut(s) 2839, 2965, 3666
BsaBI GATNNNNATC 1 cut(s) 1411
BsaI GGTCTC 2 cut(s) 1809, 2424
BsaWI WCCGGW 2 cut(s) 1100, 3002
BsaXI ACNNNNNCTCC 8 cut(s) 65, 95, 3417, 3447, 3911, 3941, 4275, 4305
Bsc4I CCNNNNNNNGG 9 cut(s) 611, 631, 1240, 1899, 1990, 2708, 3281, 3665, 4280
Bse118I RCCGGY 1 cut(s) 1100
Bse21I CCTNAGG 1 cut(s) 3077
Bse3DI GCAATG 5 cut(s) 483, 918, 1782, 2551, 2745
Bse8I GATNNNNATC 1 cut(s) 1411
BseBI CCWGG 8 cut(s) 1262, 2203, 2379, 2446, 2853, 3183, 3498, 4200
BseJI GATNNNNATC 1 cut(s) 1411
BseLI CCNNNNNNNGG 9 cut(s) 611, 631, 1240, 1899, 1990, 2708, 3281, 3665, 4280
BseMI GCAATG 5 cut(s) 483, 918, 1782, 2551, 2745
BseRI GAGGAG 3 cut(s) 933, 2721, 3438
BseSI GKGCMC 1 cut(s) 1726
BseYI CCCAGC 3 cut(s) 641, 1997, 3986
BsgI GTGCAG 2 cut(s) 3343, 3956
Bsh1236I CGCG 1 cut(s) 1270
Bsh1285I CGRYCG 2 cut(s) 286, 423
BshFI GGCC 9 cut(s) 881, 1026, 1066, 1506, 1565, 2414, 2470, 2851, 3456
BshNI GGYRCC 1 cut(s) 801
BshTI ACCGGT 1 cut(s) 1100
BsiEI CGRYCG 2 cut(s) 286, 423
BsiHKAI GWGCWC 4 cut(s) 1479, 1726, 1773, 3811
BsiHKCI CYCGRG 3 cut(s) 875, 1316, 3442
BsiSI CCGG 6 cut(s) 1101, 2620, 2838, 2964, 3003, 3665
BslFI GGGAC 6 cut(s) 585, 1278, 1586, 3459, 3866, 4284
BslI CCNNNNNNNGG 9 cut(s) 611, 631, 1240, 1899, 1990, 2708, 3281, 3665, 4280
BsmAI GTCTC 5 cut(s) 938, 1199, 1809, 2424, 3161
BsmBI CGTCTC 1 cut(s) 1199
BsmFI GGGAC 6 cut(s) 585, 1278, 1586, 3459, 3866, 4284
BsmI GAATGC 2 cut(s) 559, 2668
BsnI GGCC 9 cut(s) 881, 1026, 1066, 1506, 1565, 2414, 2470, 2851, 3456
Bso31I GGTCTC 2 cut(s) 1809, 2424
BsoBI CYCGRG 3 cut(s) 875, 1316, 3442
Bsp1286I GDGCHC 5 cut(s) 1479, 1726, 1773, 2062, 3811
Bsp1720I GCTNAGC 2 cut(s) 1767, 3650
BspACI CCGC 5 cut(s) 909, 1268, 1385, 2883, 3457
BspANI GGCC 9 cut(s) 881, 1026, 1066, 1506, 1565, 2414, 2470, 2851, 3456
BspFNI CGCG 1 cut(s) 1270
BspHI TCATGA 1 cut(s) 897
BspLI GGNNCC 8 cut(s) 601, 803, 1065, 1266, 2969, 3083, 3447, 4272
BspMAI CTGCAG 3 cut(s) 2030, 2311, 2820
BspMI ACCTGC 3 cut(s) 17, 1204, 1938
BspOI GCTAGC 2 cut(s) 845, 1947
BspPI GGATC 7 cut(s) 197, 660, 1975, 2009, 2885, 4208, 4250
BspQI GCTCTTC 1 cut(s) 2319
BspT107I GGYRCC 1 cut(s) 801
BspTNI GGTCTC 2 cut(s) 1809, 2424
BsrDI GCAATG 5 cut(s) 483, 918, 1782, 2551, 2745
BsrFI RCCGGY 1 cut(s) 1100
BssAI RCCGGY 1 cut(s) 1100
BssT1I CCWWGG 2 cut(s) 805, 3798
Bst2UI CCWGG 8 cut(s) 1262, 2203, 2379, 2446, 2853, 3183, 3498, 4200
Bst6I CTCTTC 7 cut(s) 123, 1223, 2319, 2339, 2412, 3413, 4310
BstC8I GCNNGC 4 cut(s) 843, 1142, 1397, 1945
BstDSI CCRYGG 1 cut(s) 3951
BstEII GGTNACC 2 cut(s) 443, 1351
BstFNI CGCG 1 cut(s) 1270
BstH2I RGCGCY 1 cut(s) 4199
BstHHI GCGC 2 cut(s) 2701, 4198
BstMAI GTCTC 5 cut(s) 938, 1199, 1809, 2424, 3161
BstMCI CGRYCG 2 cut(s) 286, 423
BstMWI GCNNNNNNNGC 3 cut(s) 494, 1940, 3299
BstNI CCWGG 8 cut(s) 1262, 2203, 2379, 2446, 2853, 3183, 3498, 4200
BstNSI RCATGY 1 cut(s) 2776
BstPI GGTNACC 2 cut(s) 443, 1351
BstSFI CTRYAG 5 cut(s) 2026, 2307, 2352, 2816, 3601
BstSLI GKGCMC 1 cut(s) 1726
BstUI CGCG 1 cut(s) 1270
BstV2I GAAGAC 3 cut(s) 129, 728, 1679
BstX2I RGATCY 2 cut(s) 2001, 3676
BstXI CCANNNNNNTGG 4 cut(s) 812, 2127, 3537, 4137
BstYI RGATCY 2 cut(s) 2001, 3676
Bsu36I CCTNAGG 1 cut(s) 3077
BsuI GTATCC 3 cut(s) 976, 1552, 4244
BsuRI GGCC 9 cut(s) 881, 1026, 1066, 1506, 1565, 2414, 2470, 2851, 3456
BtgI CCRYGG 1 cut(s) 3951
BtsI GCAGTG 3 cut(s) 2138, 2259, 3749
BveI ACCTGC 3 cut(s) 17, 1204, 1938
Cac8I GCNNGC 4 cut(s) 843, 1142, 1397, 1945
CaiI CAGNNNCTG 2 cut(s) 1421, 4280
CciI TCATGA 1 cut(s) 897
CfoI GCGC 2 cut(s) 2701, 4198
Cfr10I RCCGGY 1 cut(s) 1100
Cfr13I GGNCC 8 cut(s) 599, 1064, 1265, 2031, 2469, 2588, 3446, 4271
Csp6I GTAC 5 cut(s) 30, 713, 816, 2365, 3994
CspAI ACCGGT 1 cut(s) 1100
CspCI CAANNNNNGTGG 4 cut(s) 1817, 1852, 2629, 2664
CviQI GTAC 5 cut(s) 30, 713, 816, 2365, 3994
DraI TTTAAA 1 cut(s) 1441
EaeI YGGCCR 2 cut(s) 1024, 2412
Eam1104I CTCTTC 7 cut(s) 123, 1223, 2319, 2339, 2412, 3413, 4310
EarI CTCTTC 7 cut(s) 123, 1223, 2319, 2339, 2412, 3413, 4310
EciI GGCGGA 1 cut(s) 1400
Ecl136II GAGCTC 2 cut(s) 1477, 1771
Eco130I CCWWGG 2 cut(s) 805, 3798
Eco147I AGGCCT 2 cut(s) 1565, 2851
Eco24I GRGCYC 3 cut(s) 1479, 1773, 2062
Eco31I GGTCTC 2 cut(s) 1809, 2424
Eco32I GATATC 2 cut(s) 1228, 3025
Eco47I GGWCC 6 cut(s) 599, 1265, 2031, 2588, 3446, 4271
Eco53kI GAGCTC 2 cut(s) 1477, 1771
Eco57I CTGAAG 9 cut(s) 141, 287, 1185, 1959, 1990, 2208, 2424, 3437, 4037
Eco81I CCTNAGG 1 cut(s) 3077
Eco88I CYCGRG 3 cut(s) 875, 1316, 3442
Eco91I GGTNACC 2 cut(s) 443, 1351
EcoICRI GAGCTC 2 cut(s) 1477, 1771
EcoO109I RGGNCCY 2 cut(s) 599, 1064
EcoO65I GGTNACC 2 cut(s) 443, 1351
EcoRI GAATTC 1 cut(s) 1424
EcoRII CCWGG 8 cut(s) 1260, 2201, 2377, 2444, 2851, 3181, 3496, 4198
EcoRV GATATC 2 cut(s) 1228, 3025
EcoT14I CCWWGG 2 cut(s) 805, 3798
EcoT38I GRGCYC 3 cut(s) 1479, 1773, 2062
ErhI CCWWGG 2 cut(s) 805, 3798
Esp3I CGTCTC 1 cut(s) 1199
FalI AAGNNNNNCTT 2 cut(s) 876, 908
FaqI GGGAC 6 cut(s) 585, 1278, 1586, 3459, 3866, 4284
FauI CCCGC 1 cut(s) 2876
FauNDI CATATG 2 cut(s) 169, 4042
FbaI TGATCA 2 cut(s) 409, 2037
FblI GTMKAC 2 cut(s) 1257, 1611
FriOI GRGCYC 3 cut(s) 1479, 1773, 2062
GlaI GCGC 2 cut(s) 2700, 4197
GsaI CCCAGC 3 cut(s) 645, 2001, 3990
GsuI CTGGAG 3 cut(s) 2040, 3519, 4302
HaeII RGCGCY 1 cut(s) 4199
HaeIII GGCC 9 cut(s) 881, 1026, 1066, 1506, 1565, 2414, 2470, 2851, 3456
HapII CCGG 6 cut(s) 1101, 2620, 2838, 2964, 3003, 3665
HhaI GCGC 2 cut(s) 2701, 4198
Hin6I GCGC 2 cut(s) 2699, 4196
HinP1I GCGC 2 cut(s) 2699, 4196
HincII GTYRAC 5 cut(s) 889, 1527, 1747, 3201, 3222
HindII GTYRAC 5 cut(s) 889, 1527, 1747, 3201, 3222
HindIII AAGCTT 3 cut(s) 269, 3575, 3633
HinfI GANTC 9 cut(s) 217, 491, 1889, 2022, 2441, 2486, 2669, 3053, 3786
HpaI GTTAAC 2 cut(s) 1747, 3222
HpaII CCGG 6 cut(s) 1101, 2620, 2838, 2964, 3003, 3665
HphI GGTGA 7 cut(s) 1061, 2142, 3024, 3142, 3359, 3787, 4184
HpyAV CCTTC 9 cut(s) 219, 658, 843, 895, 1829, 2460, 2789, 2939, 3829
HpyCH4IV ACGT 2 cut(s) 1208, 1278
HpyF10VI GCNNNNNNNGC 3 cut(s) 494, 1940, 3299
HpySE526I ACGT 2 cut(s) 1208, 1278
HspAI GCGC 2 cut(s) 2699, 4196
Ksp22I TGATCA 2 cut(s) 409, 2037
KspAI GTTAAC 2 cut(s) 1747, 3222
LguI GCTCTTC 1 cut(s) 2319
LmnI GCTCC 7 cut(s) 66, 1118, 1204, 1706, 3087, 3646, 3932
LweI GCATC 6 cut(s) 307, 1301, 2506, 3154, 3280, 3531
MaeII ACGT 2 cut(s) 1208, 1278
MfeI CAATTG 2 cut(s) 3225, 3306
MflI RGATCY 2 cut(s) 2001, 3676
MhlI GDGCHC 5 cut(s) 1479, 1726, 1773, 2062, 3811
MlsI TGGCCA 1 cut(s) 1026
MluNI TGGCCA 1 cut(s) 1026
MlyI GAGTC 2 cut(s) 2031, 3062
MmeI TCCRAC 7 cut(s) 44, 630, 973, 1216, 2008, 3892, 4241
Mox20I TGGCCA 1 cut(s) 1026
MroXI GAANNNNTTC 1 cut(s) 350
MscI TGGCCA 1 cut(s) 1026
MseI TTAA 9 cut(s) 669, 1440, 1620, 1746, 2561, 2757, 3221, 3918, 4191
MslI CAYNNNNRTG 4 cut(s) 1415, 3263, 3539, 3733
Msp20I TGGCCA 1 cut(s) 1026
MspA1I CMGCKG 2 cut(s) 2883, 3302
MspI CCGG 6 cut(s) 1101, 2620, 2838, 2964, 3003, 3665
MunI CAATTG 2 cut(s) 3225, 3306
Mva1269I GAATGC 2 cut(s) 559, 2668
MvaI CCWGG 8 cut(s) 1262, 2203, 2379, 2446, 2853, 3183, 3498, 4200
MvnI CGCG 1 cut(s) 1270
MwoI GCNNNNNNNGC 3 cut(s) 494, 1940, 3299
NciI CCSGG 3 cut(s) 2839, 2965, 3666
NdeI CATATG 2 cut(s) 169, 4042
NheI GCTAGC 2 cut(s) 841, 1943
NlaIV GGNNCC 8 cut(s) 601, 803, 1065, 1266, 2969, 3083, 3447, 4272
NmuCI GTSAC 5 cut(s) 415, 1417, 1497, 2800, 3040
NspI RCATGY 1 cut(s) 2776
PagI TCATGA 1 cut(s) 897
PceI AGGCCT 2 cut(s) 1565, 2851
PciSI GCTCTTC 1 cut(s) 2319
PcsI WCGNNNNNNNCGW 1 cut(s) 1218
PctI GAATGC 2 cut(s) 559, 2668
PdmI GAANNNNTTC 1 cut(s) 350
PfeI GAWTC 7 cut(s) 217, 491, 1889, 2441, 2486, 2669, 3786
PflMI CCANNNNNTGG 3 cut(s) 1899, 1990, 4280
PfoI TCCNGGA 3 cut(s) 2377, 3181, 3496
PinAI ACCGGT 1 cut(s) 1100
PleI GAGTC 2 cut(s) 2030, 3061
PpsI GAGTC 2 cut(s) 2030, 3061
PpuMI RGGWCCY 1 cut(s) 599
PsiI TTATAA 1 cut(s) 3774
Psp124BI GAGCTC 2 cut(s) 1479, 1773
Psp5II RGGWCCY 1 cut(s) 599
Psp6I CCWGG 8 cut(s) 1260, 2201, 2377, 2444, 2851, 3181, 3496, 4198
PspEI GGTNACC 2 cut(s) 443, 1351
PspFI CCCAGC 3 cut(s) 641, 1997, 3986
PspGI CCWGG 8 cut(s) 1260, 2201, 2377, 2444, 2851, 3181, 3496, 4198
PspN4I GGNNCC 8 cut(s) 601, 803, 1065, 1266, 2969, 3083, 3447, 4272
PspPI GGNCC 8 cut(s) 599, 1064, 1265, 2031, 2469, 2588, 3446, 4271
PspPPI RGGWCCY 1 cut(s) 599
PstI CTGCAG 3 cut(s) 2030, 2311, 2820
PstNI CAGNNNCTG 2 cut(s) 1421, 4280
PsuI RGATCY 2 cut(s) 2001, 3676
PvuII CAGCTG 1 cut(s) 3302
RsaI GTAC 5 cut(s) 31, 714, 817, 2366, 3995
RsaNI GTAC 5 cut(s) 30, 713, 816, 2365, 3994
RseI CAYNNNNRTG 4 cut(s) 1415, 3263, 3539, 3733
SacI GAGCTC 2 cut(s) 1479, 1773
SapI GCTCTTC 1 cut(s) 2319
SaqAI TTAA 9 cut(s) 669, 1440, 1620, 1746, 2561, 2757, 3221, 3918, 4191
Sau96I GGNCC 8 cut(s) 599, 1064, 1265, 2031, 2469, 2588, 3446, 4271
SchI GAGTC 2 cut(s) 2031, 3062
SduI GDGCHC 5 cut(s) 1479, 1726, 1773, 2062, 3811
SfaNI GCATC 6 cut(s) 307, 1301, 2506, 3154, 3280, 3531
SfcI CTRYAG 5 cut(s) 2026, 2307, 2352, 2816, 3601
SinI GGWCC 6 cut(s) 599, 1265, 2031, 2588, 3446, 4271
SmiMI CAYNNNNRTG 4 cut(s) 1415, 3263, 3539, 3733
SmlI CTYRAG 3 cut(s) 548, 3158, 3545
SmoI CTYRAG 3 cut(s) 548, 3158, 3545
SseBI AGGCCT 2 cut(s) 1565, 2851
SsiI CCGC 5 cut(s) 909, 1268, 1385, 2883, 3457
SspI AATATT 3 cut(s) 870, 1379, 3482
SstI GAGCTC 2 cut(s) 1479, 1773
StuI AGGCCT 2 cut(s) 1565, 2851
StyI CCWWGG 2 cut(s) 805, 3798
TaiI ACGT 2 cut(s) 1211, 1281
TatI WGTACW 1 cut(s) 3993
TauI GCSGC 1 cut(s) 3459
TfiI GAWTC 7 cut(s) 217, 491, 1889, 2441, 2486, 2669, 3786
Tru1I TTAA 9 cut(s) 669, 1440, 1620, 1746, 2561, 2757, 3221, 3918, 4191
Tru9I TTAA 9 cut(s) 669, 1440, 1620, 1746, 2561, 2757, 3221, 3918, 4191
TseFI GTSAC 5 cut(s) 415, 1417, 1497, 2800, 3040
Tsp45I GTSAC 5 cut(s) 415, 1417, 1497, 2800, 3040
TspGWI ACGGA 1 cut(s) 3968
Van91I CCANNNNNTGG 3 cut(s) 1899, 1990, 4280
VneI GTGCAC 1 cut(s) 1722
VpaK11BI GGWCC 6 cut(s) 599, 1265, 2031, 2588, 3446, 4271
XapI RAATTY 6 cut(s) 707, 957, 1424, 2115, 2958, 2997
XbaI TCTAGA 1 cut(s) 1358
XceI RCATGY 1 cut(s) 2776
XcmI CCANNNNNNNNNTGG 4 cut(s) 1900, 2245, 2710, 4224
XmiI GTMKAC 2 cut(s) 1257, 1611
XmnI GAANNNNTTC 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.