MD09G1204000.v1.1
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002725
Physical Location & Seq
Reverse (-)
19192996 .. 19201943
8948 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1204000.v1.1.491

Sequence Viewer

Length: 4470 bp
ATGGATGGAACGGAGAGAGTAAAGGACTCGCATGAAAGGCACCGTAGCTTCAGCAGAAGCGTGGAGGATGTGATTTTAAGTGCCACACACTCTCGTCGAAACAGTCGAATGGAAGAAGAAGAAGAAGCTCTGACATGGGCTGCCATTGAGAGACTGCCCACATATAATCGGCTAAGAACAAGCATCATCAAATCCTTTGTGGAAACCGATGCTCAAGGAAATAACAACAACAAAGTGGTGCGTAAGGAAGTAGATGTTCTGAAGCTCGACCTAATCGACCGTCAGAAATTCATCGACGGCACTTTTAAGATTGCCGAGGAAGACAATGAGAGGTTCTTGAAGAAGTTTAGAAGTAGAATTGATGAGGTGGGTATCAAACTACCTACAGTGGAAGTTAGGTTTGAGCACTTGACAGTTGAAGCCGATAGCCATATTGGCAGCAGAGCTCTTCCCACTCTCCCAAATGTTACTCGGAACATTGCAGAATCAGCTCTTGGCTTAATTGGGATTAGATTAGCTAAAAAAACAAATCAAACAATTCTCAAAGATGCCTCTGGCATTGTTAAACCGTCGAGGATGACCCTTTTATTAGGTCCTCCATCCTCTGGAAAAACAACACTTTTGCTGGCACTAGCTGGGAAATTGGAGCCAAGCTTGAAGGTCAAAGGAGATATCACTTACAATGGGTACAAGCTACATGATTTTGTGCCACAGAAGACATCTGCGTATATTAGCCAAAATGATGTTCATATAGGAGAAATGACAGTCAAAGAAACTCTAGATTTTTCCGCTAGGTGCCAAGGGGTTGGGACTAGATATGAACTTCTTTGTGAGCTTGCTAGAAGAGAAAAGGACGCTGGTATATTTCCTGAGGTAGATGTTGACCTTTTCATGAAGGCTACATCAATGGGAGGAGTTGAGAGTAGTCTCATTACTGACTATACTCTCAGAATTTTGGGGCTCGATATATGCAAGGATACAATTGTTGGAGATGAGATGCAGAGAGGGATATCTGGTGGGCAGAAAAAACGAGTAACCACAGGTGAGATGATTGTTGGGCCTACAAAAACATTGTTCATGGATGAGATATCAATGGGTCTAGATAGCTCTACGACATATCAAATAGTGAAGTGCTTGCAGCAAATCGTACACATTACCGAGGCCACAATTTTGATGTCCCTACTCCAACCTGGTCCTGAGACATTTGATCTCTTTGATGATATCATCCTTTTGTCAAAGGGCCATATTGTCTACCAGGGGCCACGCGAGCTTATATTGGAGTTCTTTGATAGTTGTGGATTTCGATGCCCTGAACGAAAGGGAACTGCTGAGTTCTTACAAGAGGTAACCTCAAGGAAAGACCAAGAGCAATATTGGGCCGACAGAGGCAAGCCATACCAATACATATCAGTCACTGAATTCGCAAACCAGTTCAAGCGCTTCCATGTGGGAATGAGTATCCATAATGAGCTCTCAATCCCTTACAATAAGTCCCGAGGGCATAGAGCAGCCCTTGTTTTCACAAGATATTCAATACCCAAATTGGAACTTCTCAAGGCCTGTTTTGCCAAGGAATGGCTGCTGATAAAAAGAAACTCTTTTGTTTACATTTTCAAGATGGTTCAAATTAGTATAGTGGCAGTTGTAGCAACTACCGTATTCTTAAGGACCCAAATGCACACCCAAAATGAAAATGATGGTGCATTGTTTATCAGTGCAATTATATTTTCCCTGGTCACTAACTTGTTTAATGGTTTCTCTGAGCTCTCACTGACCATTGCAAGACTCCCTGTATTTTACAAGCACAGAGACCTTCTTTTCCACCCTGCTTGGACTTTCACTCTCCCATGTGTCCTACTCCGGATTCCTATATCCCTTTTAGAATCCACTATTTGGGTTGGCATTACATATTACACAATTGGGTTTGCACCTGAAGCTAGCAGATTTTTCAACCAACTATTGCTGTTTTTTCTGATCCAACAAATGGCTGCTGGGATGTTTAGGCTTATCGCTGGAGTCTGCAGGACCATGATCATTTCCAACACAGGCGGGAGTATCAGTCTGATGATTGTTTTTATACTTGGAGGTTTCTTAATTCCTCGAGGTGAAATTCCAAAGTGGTGGATGTGGAGCTACTGGATTTCACCTATGGCATATGGCTTCAATGCTCTTGCTGTAAACGAAATGTTTGCACCGAGGTGGATGAACAAACTGGCTTCAGATAATGCCACCAGATTGGGTGTGGCAGTGCTTGAGAACTTTGGTATTTTCCCTGACAGAAACTGGGTTTGGATTGGATCTGCAGCTCTTGTCGGATATGCAGTTTTCTTCAACATCCTTTTCACACTCGTACTTATGTACCTGAATCCTCTTGGAAAGCCACAGCCTATGATATCTGAAGAAGTAGCGGCTGAAATAAGAGCAGATGAAGAAGAATCGAAGGATGACCAGAATTTCAGAAGACCCAAATTAAAGACAGATTCAATTTTTCGATCATTTTCTTCTTCTGATGGAAACAATTCAGGAGAAATGCAAATATGGAGATTGGGCAGTCGATCAAATGCGAATAGACTTGGTAGAAATGCTAATTCGTCTCTTGAAGCAGGAAATGTTGTTACCCTAAAGAGAGGAATGGTTCTTCCCTATACTCCTCTTGCAATGTCCTTCGACAGTGTTAATTACTATGTGGACATGCCCCCCAAAATGAAGGAAGAAGAAGTAAAAGAGGACAGGCTTCAGCTACTTTGTGAAGTAACTGGTGCCTTTAGGCCTGGGGTCTTGACAGCTCTAATGGGAGTCAGCGGGGCGGGGAAGACAACTTTGATGGATGTCTTAGCAGGAAGAAAAACAGGTGGCTACATTGAAGGTGATATTAGAATTTCCGGGTACCCTAAGAAACAAGAAACCTTTGCCAGAATTTCTGGTTATTGTGAACAAAATGATATCCACTCACCCCAAGTCACTGTCAAGGATTCATTGATTTATTCAGCTTTCCTTCGGCTCCCTAAAGAAGTCACCAAAGAGGAAAAGATGACCTTTGTAGATGAAGTAATGGAACTGGTTGAGCTAGGTGGTCTCAAAGATGCTTTAGTGGGGCTTCCAGGAGTTACAGGGTTATCAACAGAACAGAGAAAGAGGTTGACAATTGCAGTAGAGCTCGTTGCTAATCCCTCGATCATTTTCATGGATGAGCCAACATCAGGTCTTGATGCAAGGGCAGCTGCCATTGTTATGAGGGTGGTTAGAAATACCGTGGACACTGGTAGAACAGTTGTCTGCACAATTCATCAGCCTAGCATAGATATCTTTGAGGCCTTTGATGAGCTGCTGCTCATGAAGAGAGGAGGACAGTTGATATACTCCGGACCATTGGGCCGCAATTCTCACAAGGTCGTGGAATATTTTGAGGCAGTTCCTGGTGTTCCAAAAATTAAAGAAAAGTATAATCCAGCAACATGGATGCTTGAGGTGAGTTCGACCGCAACTGAGCTCCGGCTTGAAATCGACTTTGCTCAACACTACAAGTCATCTTCCTTGTACCAGAGAAACAAAGCTTTAGTGACAGAGTTAAGCATACCACCACCAGGAGCAAATGATCTTTATTTTCCATCTCAGTATTCTCAATCAATGTGGGAGCAGTTCAAATGTTGCCTTTGGAAGCACTGGTGGACTTACTGGAGAAGTCCTGATTATATCCTTGTTAGGTTCTTTTTTACCTTTGCCTCTGCCCTCCTGGTTGGATCTATTTTCTGGAAGGTTGGCGCCAAAAGAGAAAGCTCAGCTGACCTGACCATGATTATTGGAGCCATGTATAATGCTGTACTTTCCATTGGAATTAATAACTGCTCAACAGTGCAGCCGGTGGTCGCCACTGAAAGAGTAGTATTTTATCGAGAACGAGCTGCTGGGATGTACTCTGCCATACCTTATGCACTAGCACAGGTTGTTGTTGAAATACCGTATGTGTTTGTTCAAACTGTATATTATGTGCTGATTGTGTATGCTATGGTGAGCTTTCAATGGACGGCGGAAAAATTCTTCTGGTTCTTCTTTGTCAACTTCATCTCATTCCTTTATTTCACATATTACGGAATGATGACTGTCGCCATCTCACCAAACCATCAAGTAGCAGCCATATTGGCATCAACGTTTTATTCATTCTTCAACCTCTTCTCGGGTTTCTTCATACCAAGGCCGAAAATACCCAAGTGGTGGATTTGGTATTACTGGATATGCCCGGTAGCGTGGACGGTGTATGGATTGATTGTGTCACAATATGGTGATGTTGAGGACACCATTAAAGCACCTGGGTTAACACCTGACCCAACTGTGAAATGGTATGTAGAAAACTATTTTGGATATAATCCAAATTTCATGGGACCAGTTGCGGGAGTTCTTGTTGGCTTCGCGCTCTTCTTTGCCTTCATGTTTGCCTGTTGTATAAAGATACTCAATTTCCAAATTAGATAG

Protein Analysis

1490

Amino Acids

168.63

Weight (kDa)

8.26

Isoelectric Point (pI)

35.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 106 - 156 4.9e-10 ABC-transporter N-terminal
ABC_tran PF00005 181 - 362 6.4e-14 ABC transporter
ABC2_membrane PF01061 517 - 728 1.1e-47 ABC-2 type transporter
PDR_assoc PF08370 734 - 798 4.9e-25 Plant PDR ABC transporter associated
ABC_tran PF00005 918 - 1067 1.9e-19 ABC transporter
ABC2_membrane PF01061 1212 - 1426 1.7e-61 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 2100
Acc65I GGTACC 1 cut(s) 2886
AccB1I GGYRCC 5 cut(s) 39, 795, 2759, 2886, 3761
AccB7I CCANNNNNTGG 5 cut(s) 605, 1575, 1893, 1984, 4212
AccI GTMKAC 1 cut(s) 1251
AccII CGCG 2 cut(s) 1266, 4409
AccIII TCCGGA 2 cut(s) 1860, 3362
AciI CCGC 9 cut(s) 789, 2049, 2408, 2802, 2807, 3376, 3480, 4028, 4388
AclI AACGTT 1 cut(s) 4148
AclWI GGATC 3 cut(s) 1969, 2305, 3748
AcsI RAATTY 9 cut(s) 287, 951, 1418, 2109, 2452, 2877, 2916, 4034, 4369
AcuI CTGAAG 6 cut(s) 34, 281, 1953, 2202, 2418, 2720
AcyI GRCGYC 1 cut(s) 3762
AfaI GTAC 8 cut(s) 689, 1149, 2352, 2360, 2888, 3539, 3822, 3914
AfeI AGCGCT 1 cut(s) 1439
AfiI CCNNNNNNNGG 9 cut(s) 605, 1575, 1893, 1984, 3200, 3584, 4174, 4212, 4388
AflII CTTAAG 1 cut(s) 1663
AjnI CCWGG 9 cut(s) 1189, 1254, 1731, 2770, 3100, 3415, 3583, 3732, 4306
AjuI GAANNNNNNNTTGG 8 cut(s) 477, 509, 729, 761, 2272, 2304, 4338, 4370
AleI CACNNNNGTG 1 cut(s) 2197
Alw21I GWGCWC 6 cut(s) 408, 448, 1473, 1767, 3159, 3492
Alw26I GTCTC 6 cut(s) 145, 932, 1193, 1803, 2598, 3080
AlwI GGATC 3 cut(s) 1969, 2305, 3748
AlwNI CAGNNNCTG 3 cut(s) 1415, 2283, 3416
Ama87I CYCGRG 3 cut(s) 1494, 2100, 4174
Aor13HI TCCGGA 2 cut(s) 1860, 3362
Aor51HI AGCGCT 1 cut(s) 1439
ApoI RAATTY 9 cut(s) 287, 951, 1418, 2109, 2452, 2877, 2916, 4034, 4369
AseI ATTAAT 1 cut(s) 3837
Asp700I GAANNNNTTC 1 cut(s) 2518
Asp718I GGTACC 1 cut(s) 2886
AspLEI GCGC 3 cut(s) 1440, 3764, 4411
AsuC2I CCSGG 2 cut(s) 2884, 4238
AsuNHI GCTAGC 1 cut(s) 1937
AvaI CYCGRG 3 cut(s) 1494, 2100, 4174
AvaII GGWCC 6 cut(s) 593, 1193, 1668, 2025, 3365, 4379
AxyI CCTNAGG 1 cut(s) 870
BaeI ACNNNNGTAYC 4 cut(s) 969, 1002, 2342, 2375
BanI GGYRCC 5 cut(s) 39, 795, 2759, 2886, 3761
BanII GRGCYC 6 cut(s) 448, 963, 1473, 1767, 3159, 3492
BbsI GAAGAC 4 cut(s) 327, 722, 2467, 2819
Bbv12I GWGCWC 6 cut(s) 408, 448, 1473, 1767, 3159, 3492
BccI CCATC 8 cut(s) 607, 1612, 1691, 2504, 2818, 3616, 4115, 4128
BceAI ACGGC 2 cut(s) 313, 4041
BcgI CGANNNNNNTGC 2 cut(s) 2171, 2205
BciT130I CCWGG 9 cut(s) 1191, 1256, 1733, 2772, 3102, 3417, 3585, 3734, 4308
BciVI GTATCC 2 cut(s) 970, 1469
BclI TGATCA 1 cut(s) 2031
BcnI CCSGG 2 cut(s) 2884, 4238
BcoDI GTCTC 6 cut(s) 145, 932, 1193, 1803, 2598, 3080
BfmI CTRYAG 3 cut(s) 384, 2020, 2301
BfoI RGCGCY 2 cut(s) 1441, 3765
BfrI CTTAAG 1 cut(s) 1663
BfuI GTATCC 2 cut(s) 970, 1469
BglI GCCNNNNNGGC 2 cut(s) 435, 4139
BlpI GCTNAGC 1 cut(s) 3778
Bme18I GGWCC 6 cut(s) 593, 1193, 1668, 2025, 3365, 4379
BmeT110I CYCGRG 3 cut(s) 1494, 2100, 4174
BmrI ACTGGG 1 cut(s) 2293
BmsI GCATC 9 cut(s) 192, 199, 538, 987, 1295, 3073, 3199, 3450, 4151
BmtI GCTAGC 1 cut(s) 1941
BmuI ACTGGG 1 cut(s) 2293
BpiI GAAGAC 4 cut(s) 327, 722, 2467, 2819
BplI GAGNNNNNCTC 2 cut(s) 1334, 1366
BpmI CTGGAG 2 cut(s) 2034, 3697
Bpu1102I GCTNAGC 1 cut(s) 3778
BpuEI CTTGAG 5 cut(s) 198, 1336, 1538, 2273, 3485
BpuMI CCSGG 2 cut(s) 2884, 4238
BsaHI GRCGYC 1 cut(s) 3762
BsaI GGTCTC 2 cut(s) 1803, 3080
BsaWI WCCGGW 2 cut(s) 1860, 3362
BsaXI ACNNNNNCTCC 4 cut(s) 2044, 2074, 4383, 4413
Bsc4I CCNNNNNNNGG 9 cut(s) 605, 1575, 1893, 1984, 3200, 3584, 4174, 4212, 4388
Bse118I RCCGGY 1 cut(s) 3859
Bse21I CCTNAGG 1 cut(s) 870
Bse3DI GCAATG 3 cut(s) 477, 1776, 2664
BseAI TCCGGA 2 cut(s) 1860, 3362
BseBI CCWGG 9 cut(s) 1191, 1256, 1733, 2772, 3102, 3417, 3585, 3734, 4308
BseLI CCNNNNNNNGG 9 cut(s) 605, 1575, 1893, 1984, 3200, 3584, 4174, 4212, 4388
BseMI GCAATG 3 cut(s) 477, 1776, 2664
BseMII CTCAG 8 cut(s) 861, 961, 1188, 1320, 1752, 3477, 3626, 3792
BseRI GAGGAG 3 cut(s) 927, 2640, 3357
BseYI CCCAGC 3 cut(s) 635, 1991, 3905
BsgI GTGCAG 2 cut(s) 3262, 3875
Bsh1236I CGCG 2 cut(s) 1266, 4409
Bsh1285I CGRYCG 2 cut(s) 280, 3480
BshNI GGYRCC 5 cut(s) 39, 795, 2759, 2886, 3761
BsiEI CGRYCG 2 cut(s) 280, 3480
BsiHKAI GWGCWC 6 cut(s) 408, 448, 1473, 1767, 3159, 3492
BsiHKCI CYCGRG 3 cut(s) 1494, 2100, 4174
BsiSI CCGG 6 cut(s) 1861, 2883, 3363, 3493, 3860, 4238
BslFI GGGAC 4 cut(s) 823, 1162, 1477, 4392
BslI CCNNNNNNNGG 9 cut(s) 605, 1575, 1893, 1984, 3200, 3584, 4174, 4212, 4388
BsmAI GTCTC 6 cut(s) 145, 932, 1193, 1803, 2598, 3080
BsmBI CGTCTC 1 cut(s) 2598
BsmFI GGGAC 4 cut(s) 823, 1162, 1477, 4392
Bso31I GGTCTC 2 cut(s) 1803, 3080
BsoBI CYCGRG 3 cut(s) 1494, 2100, 4174
Bsp1286I GDGCHC 7 cut(s) 408, 448, 963, 1473, 1767, 3159, 3492
Bsp13I TCCGGA 2 cut(s) 1860, 3362
Bsp143I GATC 9 cut(s) 1207, 1974, 2031, 2297, 2492, 2555, 3174, 3595, 3740
Bsp1720I GCTNAGC 1 cut(s) 3778
BspACI CCGC 9 cut(s) 789, 2049, 2408, 2802, 2807, 3376, 3480, 4028, 4388
BspCNI CTCAG 8 cut(s) 862, 960, 1189, 1321, 1753, 3478, 3625, 3791
BspEI TCCGGA 2 cut(s) 1860, 3362
BspFNI CGCG 2 cut(s) 1266, 4409
BspHI TCATGA 2 cut(s) 891, 3333
BspMAI CTGCAG 2 cut(s) 2024, 2305
BspOI GCTAGC 1 cut(s) 1941
BspPI GGATC 3 cut(s) 1969, 2305, 3748
BspQI GCTCTTC 2 cut(s) 453, 4418
BspT107I GGYRCC 5 cut(s) 39, 795, 2759, 2886, 3761
BspTI CTTAAG 1 cut(s) 1663
BspTNI GGTCTC 2 cut(s) 1803, 3080
BsrDI GCAATG 3 cut(s) 477, 1776, 2664
BsrFI RCCGGY 1 cut(s) 3859
BssAI RCCGGY 1 cut(s) 3859
BssMI GATC 9 cut(s) 1207, 1974, 2031, 2297, 2492, 2555, 3174, 3595, 3740
BssNI GRCGYC 1 cut(s) 3762
BssT1I CCWWGG 3 cut(s) 799, 1569, 4190
Bst2UI CCWGG 9 cut(s) 1191, 1256, 1733, 2772, 3102, 3417, 3585, 3734, 4308
Bst6I CTCTTC 5 cut(s) 453, 838, 3332, 4175, 4418
BstACI GRCGYC 1 cut(s) 3762
BstAFI CTTAAG 1 cut(s) 1663
BstC8I GCNNGC 6 cut(s) 627, 837, 1136, 1268, 1391, 1939
BstDSI CCRYGG 1 cut(s) 3252
BstEII GGTNACC 1 cut(s) 1345
BstFNI CGCG 2 cut(s) 1266, 4409
BstH2I RGCGCY 2 cut(s) 1441, 3765
BstHHI GCGC 3 cut(s) 1440, 3764, 4411
BstKTI GATC 9 cut(s) 1210, 1977, 2034, 2300, 2495, 2558, 3177, 3598, 3743
BstMAI GTCTC 6 cut(s) 145, 932, 1193, 1803, 2598, 3080
BstMBI GATC 9 cut(s) 1207, 1974, 2031, 2297, 2492, 2555, 3174, 3595, 3740
BstMCI CGRYCG 2 cut(s) 280, 3480
BstMWI GCNNNNNNNGC 9 cut(s) 37, 435, 488, 1267, 1565, 1646, 1934, 3218, 4139
BstNI CCWGG 9 cut(s) 1191, 1256, 1733, 2772, 3102, 3417, 3585, 3734, 4308
BstNSI RCATGY 1 cut(s) 2695
BstPI GGTNACC 1 cut(s) 1345
BstSFI CTRYAG 3 cut(s) 384, 2020, 2301
BstUI CGCG 2 cut(s) 1266, 4409
BstV2I GAAGAC 4 cut(s) 327, 722, 2467, 2819
BstX2I RGATCY 2 cut(s) 2297, 3740
BstXI CCANNNNNNTGG 4 cut(s) 806, 2121, 2236, 3456
BstYI RGATCY 2 cut(s) 2297, 3740
Bsu36I CCTNAGG 1 cut(s) 870
BsuI GTATCC 2 cut(s) 970, 1469
BtgI CCRYGG 1 cut(s) 3252
BtsI GCAGTG 1 cut(s) 2253
Cac8I GCNNGC 6 cut(s) 627, 837, 1136, 1268, 1391, 1939
CaiI CAGNNNCTG 3 cut(s) 1415, 2283, 3416
CciI TCATGA 2 cut(s) 891, 3333
CfoI GCGC 3 cut(s) 1440, 3764, 4411
Cfr10I RCCGGY 1 cut(s) 3859
CseI GACGC 1 cut(s) 863
CsiI ACCWGGT 1 cut(s) 1189
Csp6I GTAC 8 cut(s) 688, 1148, 2351, 2359, 2887, 3538, 3821, 3913
CspCI CAANNNNNGTGG 4 cut(s) 1811, 1846, 3611, 3646
CviQI GTAC 8 cut(s) 688, 1148, 2351, 2359, 2887, 3538, 3821, 3913
DinI GGCGCC 1 cut(s) 3763
DpnI GATC 9 cut(s) 1209, 1976, 2033, 2299, 2494, 2557, 3176, 3597, 3742
DpnII GATC 9 cut(s) 1207, 1974, 2031, 2297, 2492, 2555, 3174, 3595, 3740
Eam1104I CTCTTC 5 cut(s) 453, 838, 3332, 4175, 4418
EarI CTCTTC 5 cut(s) 453, 838, 3332, 4175, 4418
EciI GGCGGA 1 cut(s) 4043
Ecl136II GAGCTC 5 cut(s) 446, 1471, 1765, 3157, 3490
Eco130I CCWWGG 3 cut(s) 799, 1569, 4190
Eco147I AGGCCT 3 cut(s) 1559, 2770, 3314
Eco24I GRGCYC 6 cut(s) 448, 963, 1473, 1767, 3159, 3492
Eco31I GGTCTC 2 cut(s) 1803, 3080
Eco32I GATATC 7 cut(s) 673, 1011, 1089, 1222, 2394, 2944, 3304
Eco47I GGWCC 6 cut(s) 593, 1193, 1668, 2025, 3365, 4379
Eco47III AGCGCT 1 cut(s) 1439
Eco53kI GAGCTC 5 cut(s) 446, 1471, 1765, 3157, 3490
Eco57I CTGAAG 6 cut(s) 34, 281, 1953, 2202, 2418, 2720
Eco81I CCTNAGG 1 cut(s) 870
Eco88I CYCGRG 3 cut(s) 1494, 2100, 4174
Eco91I GGTNACC 1 cut(s) 1345
EcoICRI GAGCTC 5 cut(s) 446, 1471, 1765, 3157, 3490
EcoO109I RGGNCCY 2 cut(s) 593, 1668
EcoO65I GGTNACC 1 cut(s) 1345
EcoRI GAATTC 1 cut(s) 1418
EcoRII CCWGG 9 cut(s) 1189, 1254, 1731, 2770, 3100, 3415, 3583, 3732, 4306
EcoRV GATATC 7 cut(s) 673, 1011, 1089, 1222, 2394, 2944, 3304
EcoT14I CCWWGG 3 cut(s) 799, 1569, 4190
EcoT38I GRGCYC 6 cut(s) 448, 963, 1473, 1767, 3159, 3492
EgeI GGCGCC 1 cut(s) 3763
EheI GGCGCC 1 cut(s) 3763
ErhI CCWWGG 3 cut(s) 799, 1569, 4190
Esp3I CGTCTC 1 cut(s) 2598
FalI AAGNNNNNCTT 4 cut(s) 1582, 1614, 3020, 3052
FaqI GGGAC 4 cut(s) 823, 1162, 1477, 4392
FauI CCCGC 4 cut(s) 2042, 2795, 2800, 4381
FauNDI CATATG 1 cut(s) 2155
FbaI TGATCA 1 cut(s) 2031
FblI GTMKAC 1 cut(s) 1251
FriOI GRGCYC 6 cut(s) 448, 963, 1473, 1767, 3159, 3492
GlaI GCGC 3 cut(s) 1439, 3763, 4410
GsaI CCCAGC 3 cut(s) 639, 1995, 3909
GsuI CTGGAG 2 cut(s) 2034, 3697
HaeII RGCGCY 2 cut(s) 1441, 3765
HapII CCGG 6 cut(s) 1861, 2883, 3363, 3493, 3860, 4238
HgaI GACGC 1 cut(s) 863
HhaI GCGC 3 cut(s) 1440, 3764, 4411
Hin1I GRCGYC 1 cut(s) 3762
Hin6I GCGC 3 cut(s) 1438, 3762, 4409
HinP1I GCGC 3 cut(s) 1438, 3762, 4409
HincII GTYRAC 4 cut(s) 883, 3141, 4057, 4314
HindII GTYRAC 4 cut(s) 883, 3141, 4057, 4314
HindIII AAGCTT 2 cut(s) 652, 3552
HpaI GTTAAC 1 cut(s) 4314
HpaII CCGG 6 cut(s) 1861, 2883, 3363, 3493, 3860, 4238
Hpy99I CGWCG 3 cut(s) 99, 299, 574
HpyCH4IV ACGT 1 cut(s) 4148
HpyF10VI GCNNNNNNNGC 9 cut(s) 37, 435, 488, 1267, 1565, 1646, 1934, 3218, 4139
HpySE526I ACGT 1 cut(s) 4148
Hsp92I GRCGYC 1 cut(s) 3762
HspAI GCGC 3 cut(s) 1438, 3762, 4409
KasI GGCGCC 1 cut(s) 3761
Kpn2I TCCGGA 2 cut(s) 1860, 3362
KpnI GGTACC 1 cut(s) 2890
Ksp22I TGATCA 1 cut(s) 2031
KspAI GTTAAC 1 cut(s) 4314
Kzo9I GATC 9 cut(s) 1207, 1974, 2031, 2297, 2492, 2555, 3174, 3595, 3740
LguI GCTCTTC 2 cut(s) 453, 4418
LmnI GCTCC 7 cut(s) 646, 2130, 3006, 3495, 3587, 3634, 3803
LweI GCATC 9 cut(s) 192, 199, 538, 987, 1295, 3073, 3199, 3450, 4151
MabI ACCWGGT 1 cut(s) 1189
MaeII ACGT 1 cut(s) 4148
MalI GATC 9 cut(s) 1209, 1976, 2033, 2299, 2494, 2557, 3176, 3597, 3742
MboI GATC 9 cut(s) 1207, 1974, 2031, 2297, 2492, 2555, 3174, 3595, 3740
MfeI CAATTG 3 cut(s) 981, 1917, 3144
MflI RGATCY 2 cut(s) 2297, 3740
MhlI GDGCHC 7 cut(s) 408, 448, 963, 1473, 1767, 3159, 3492
Mly113I GGCGCC 1 cut(s) 3762
MlyI GAGTC 4 cut(s) 20, 1779, 2025, 2805
MmeI TCCRAC 6 cut(s) 967, 1210, 2002, 2064, 2293, 3718
MroI TCCGGA 2 cut(s) 1860, 3362
MroXI GAANNNNTTC 1 cut(s) 2518
MslI CAYNNNNRTG 3 cut(s) 2197, 3182, 3230
MspA1I CMGCKG 3 cut(s) 2802, 3221, 3782
MspCI CTTAAG 1 cut(s) 1663
MspI CCGG 6 cut(s) 1861, 2883, 3363, 3493, 3860, 4238
MunI CAATTG 3 cut(s) 981, 1917, 3144
MvaI CCWGG 9 cut(s) 1191, 1256, 1733, 2772, 3102, 3417, 3585, 3734, 4308
MvnI CGCG 2 cut(s) 1266, 4409
MwoI GCNNNNNNNGC 9 cut(s) 37, 435, 488, 1267, 1565, 1646, 1934, 3218, 4139
NarI GGCGCC 1 cut(s) 3762
NciI CCSGG 2 cut(s) 2884, 4238
NdeI CATATG 1 cut(s) 2155
NdeII GATC 9 cut(s) 1207, 1974, 2031, 2297, 2492, 2555, 3174, 3595, 3740
NheI GCTAGC 1 cut(s) 1937
NmeAIII GCCGAG 1 cut(s) 340
NmuCI GTSAC 6 cut(s) 1411, 1735, 2959, 3013, 3559, 4269
NspI RCATGY 1 cut(s) 2695
OliI CACNNNNGTG 1 cut(s) 2197
PaeR7I CTCGAG 1 cut(s) 2100
PagI TCATGA 2 cut(s) 891, 3333
PceI AGGCCT 3 cut(s) 1559, 2770, 3314
PciSI GCTCTTC 2 cut(s) 453, 4418
PcsI WCGNNNNNNNCGW 2 cut(s) 103, 273
PdmI GAANNNNTTC 1 cut(s) 2518
PfeI GAWTC 7 cut(s) 485, 1864, 1883, 2365, 2435, 2480, 2972
PflMI CCANNNNNTGG 5 cut(s) 605, 1575, 1893, 1984, 4212
PfoI TCCNGGA 1 cut(s) 3100
PleI GAGTC 4 cut(s) 20, 1779, 2024, 2804
PluTI GGCGCC 1 cut(s) 3765
PpsI GAGTC 4 cut(s) 20, 1779, 2024, 2804
PpuMI RGGWCCY 2 cut(s) 593, 1668
PshBI ATTAAT 1 cut(s) 3837
Psp124BI GAGCTC 5 cut(s) 448, 1473, 1767, 3159, 3492
Psp1406I AACGTT 1 cut(s) 4148
Psp5II RGGWCCY 2 cut(s) 593, 1668
Psp6I CCWGG 9 cut(s) 1189, 1254, 1731, 2770, 3100, 3415, 3583, 3732, 4306
PspEI GGTNACC 1 cut(s) 1345
PspFI CCCAGC 3 cut(s) 635, 1991, 3905
PspGI CCWGG 9 cut(s) 1189, 1254, 1731, 2770, 3100, 3415, 3583, 3732, 4306
PspPPI RGGWCCY 2 cut(s) 593, 1668
PspXI VCTCGAGB 1 cut(s) 2100
PstI CTGCAG 2 cut(s) 2024, 2305
PstNI CAGNNNCTG 3 cut(s) 1415, 2283, 3416
PsuI RGATCY 2 cut(s) 2297, 3740
PvuII CAGCTG 2 cut(s) 3221, 3782
RsaI GTAC 8 cut(s) 689, 1149, 2352, 2360, 2888, 3539, 3822, 3914
RsaNI GTAC 8 cut(s) 688, 1148, 2351, 2359, 2887, 3538, 3821, 3913
RseI CAYNNNNRTG 3 cut(s) 2197, 3182, 3230
SacI GAGCTC 5 cut(s) 448, 1473, 1767, 3159, 3492
SapI GCTCTTC 2 cut(s) 453, 4418
Sau3AI GATC 9 cut(s) 1207, 1974, 2031, 2297, 2492, 2555, 3174, 3595, 3740
SchI GAGTC 4 cut(s) 20, 1779, 2025, 2805
SduI GDGCHC 7 cut(s) 408, 448, 963, 1473, 1767, 3159, 3492
SexAI ACCWGGT 1 cut(s) 1189
SfaNI GCATC 9 cut(s) 192, 199, 538, 987, 1295, 3073, 3199, 3450, 4151
SfcI CTRYAG 3 cut(s) 384, 2020, 2301
SfoI GGCGCC 1 cut(s) 3763
Sfr274I CTCGAG 1 cut(s) 2100
SinI GGWCC 6 cut(s) 593, 1193, 1668, 2025, 3365, 4379
SlaI CTCGAG 1 cut(s) 2100
SmiMI CAYNNNNRTG 3 cut(s) 2197, 3182, 3230
SmlI CTYRAG 7 cut(s) 213, 1351, 1553, 1663, 2100, 2252, 3464
SmoI CTYRAG 7 cut(s) 213, 1351, 1553, 1663, 2100, 2252, 3464
SseBI AGGCCT 3 cut(s) 1559, 2770, 3314
SsiI CCGC 9 cut(s) 789, 2049, 2408, 2802, 2807, 3376, 3480, 4028, 4388
SspDI GGCGCC 1 cut(s) 3761
SspI AATATT 2 cut(s) 1373, 3401
SstI GAGCTC 5 cut(s) 448, 1473, 1767, 3159, 3492
StuI AGGCCT 3 cut(s) 1559, 2770, 3314
StyI CCWWGG 3 cut(s) 799, 1569, 4190
TaiI ACGT 1 cut(s) 4151
TatI WGTACW 2 cut(s) 3820, 3912
TauI GCSGC 2 cut(s) 2411, 3378
TfiI GAWTC 7 cut(s) 485, 1864, 1883, 2365, 2435, 2480, 2972
TseFI GTSAC 6 cut(s) 1411, 1735, 2959, 3013, 3559, 4269
Tsp45I GTSAC 6 cut(s) 1411, 1735, 2959, 3013, 3559, 4269
TspGWI ACGGA 2 cut(s) 26, 4104
Van91I CCANNNNNTGG 5 cut(s) 605, 1575, 1893, 1984, 4212
Vha464I CTTAAG 1 cut(s) 1663
VpaK11BI GGWCC 6 cut(s) 593, 1193, 1668, 2025, 3365, 4379
VspI ATTAAT 1 cut(s) 3837
XapI RAATTY 9 cut(s) 287, 951, 1418, 2109, 2452, 2877, 2916, 4034, 4369
XbaI TCTAGA 2 cut(s) 778, 1099
XceI RCATGY 1 cut(s) 2695
XcmI CCANNNNNNNNNTGG 2 cut(s) 2239, 4332
XhoI CTCGAG 1 cut(s) 2100
XmiI GTMKAC 1 cut(s) 1251
XmnI GAANNNNTTC 1 cut(s) 2518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.