Rh2BG423600
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002725
Physical Location & Seq
Forward (+)
59604483 .. 59615957
11475 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG423600.1

Sequence Viewer

Length: 4056 bp
ATGGAGGGAACAGAGAGAGTAAAAGCAGGTACAAATCGAAGAAGCAGAAGCAGAACTCCTAGTTGGAGTATGGAGGAAGTGTTTGTGAGTGCCACGCACTCTCGAAGAAGCAGTAGAGCTGAAGACGAAGAGGCTCTGACTTGGGCTGCCATTGAGAAACTGCCAACATATGATAGACTAAGAACAGGGATCATCCAGTCCATTGTGGAGAGCGAGAATCCTCGAAGGAAAAACAACAAAGTGATGCACAAGGAAGTTGATGTTCTGAAGCTTGATGTAACCGACCGTCAAGATTTCATTGACAGGATTTTCAAGGATGCAGAGGAAGATAATGAGAAGTTCTTGAAGAAGTTCAGAAGCAGAATTGATAAGGTTGGGATTAGACTTCCCACAGTGGAAGTTAGGTTTGATCATTTGACGGTCGAAGCAGAATGCCATATTGGTAACAGAGCTCTTCCCACTCTCCCAAATGTTGCTAGGAACATTGCAGAATCAGCTCTTGGTTTGATTGGGATTACAACGGCTAAGAGAACAAATCTAACAATCCTCAAGAATGCCACTGGCATTATCAAACCATCGAGGATGGCTCTATTATTAGGTCCCCCGTCGTCAGGGAAAACAACCCTTCTGTTGGCACTAGCTGGGAAGTTGGATCCTAGCTTGAAGATTAAAGGAGAGATCACATACAATGGGTATAGGTTGAACGAATTTGTACCACAGAAGACATCTGCATATATTAGCCAAAATGATGTTCATTTAGGAGAGATGACTGTCAAAGAAACCCTAGATTTTTCGGCAAGGTGCCAAGGGGTTGGTACTCGATATGAACTTCTGTCTGAGCTAGCTAGAAGGGAAAAGGAAGCAAGAATATTTCCCGAGGCAGAAGTTGACCTTTTCATGAAGGCTACTGCAATGGGAGGAGTTGAGAGCAGTCTCATTACCGACTACACTCTCAAAATTTTGGGGCTTGATATATGCAAGGATACCATTGTTGGAAACGAAATGCAGAGAGGGATTTCTGGTGGCCAAAAGAAACGAGTAACGACAGGTGAGATGATTGTGGGGCCTACAAAAGCACTATTCATGGACGAAATATCAACCGGTCTAGATAGCTCCACCACATTTCAAATAGTGAAGTGCTTGCAGCAAATCGTGCACATCACTGAAGCTACGATCTTCATGTCGCTACTCCAACCTGCTCCTGAGACGTTTGATCTCTTCGATGATATCATCCTTTTATCGGAGGGTCAAATTGTCTACCAGGGACCGCGTGAGAACATAGTAGGGTTCTTTGAGAGCTGCGGATTTCGATGCCCCGAGCGAAAGGGAACTGCTGATTTCTTGCAAGAGGTTACCTCTAGAAAAGACCAAGAGCAATATTGGGCAGATAGAAGCAAGCCATACAGATACATATCAGTGACTGAATTCTCAAACCGATTTAAACGCTTCCATGTTGGCATGAGGCTTGAAAACGAGCTCTCAATCCCTTTTGATAAGTCACGAGGCCACAAAGCAGCTCTAGTATTCACAAGATATTCAGTATCCAAAATGGAACTTCTAAAGGCCTGTTGGGACAAGGAATGGCTACTCATAAAGAGGAACTCTTTTGTCTACATTTTTAAGACCGTCCAAATAATTATAGGGGCGTTGATAACCTCGACTGTGTTTTTGAAGACCCAAATGCACACCAGAAATGAAGATGATGGAGCAGTATATCTTGGTGCACTATTATTTACCATGATCATTAACACGTTCAATGGTTTTGCTGAGCTCTCGATGACCATTGCAAGACTACCTGTATTTTACAAGCATAGAGACCTTCTTTTCCACCCTGCTTGGACTTTCACTCTCCCAACTATCCTGCTCACTATTCCTATATCCATTGTTGAATCCACAGTTTGGATGGTCATAACATATTACACCATTGGTTTTGCACCTGAAGCTAGCAGGTTTTTCAAGCAACTATTGCTGATATTTCTGATCCAACAAATGGCTGCTGGGATCTTCAGGCTTATTGCTGGAGTCTGCAGGACCATGATCATAGCAAACACTGGTGGGGCTCTCACTTTGCTCATGGTTTTCATGCTTGGAGGTTTCATACTACCTAAAGGCGAAATTCCAAAGTGGTGGCAGTGGGGTTACTGGGTTTCACCTATGACCTATGGTTTCAATGCTATTGCTGTAAATGAAATGTTTTCTCCCAGGTGGATGAACAAATGGGCTTCAGATAATGATACCAGATTGGGTGTGGCAGTGCTTCAAAACTTCGAAGTTTTCCCTGACAAAAACTGGTTTTGGATTGGCTCTGCAGCTCTTCTGGGGTTTGCAATTCTCTTCAACATCCTCTACACTATAGCTCTCATGTACCTCAGTCCTCCTGGAAAAGCACAAGCTATTATATCTGAAGAATTGGCCGAAGAGATGGAGGGAGACCAAGAAGAATCCAGGGAAGAACCAAGACTTAGAAGGCCCAAGTCAAAGCAAGATTCATTTTCTCAGTCATTATCATCTGCTGATGCAAACAATTCAAGAGAAATGGCAATCCGGCGTATGAGCAGTCAATCCAATACCAGTGGACTAAGTAGGAATGCTGATTCTTCCCTAGAAGTAGCCAGTGGGGTTGCGCCCAAGAGAGGAATGGTTCTTCCTTTCACTCCTCTGGCAATGTCCTTTGATGATGTTAATTACTATGTGGACATGCCTCCTGAAATGAAAGAAGAAGGAGTGAGTGAGGACAGGCTGCAGCTACTTCGTGAAGTAACCGGTGCTTTTAGGCCTGGGGTCTTGACTGCTCTAATGGGGATCAGCGGGGCTGGAAAGACAACTTTGATGGATGTCTTAGCAGGAAGAAAGACTGGTGGTTACATTGAAGGCGATATTAGAATTTCCGGGTTCCCTAAGAAACAAGAAACCTTTGCAAGAATTTCTGGTTATTGTGAACAAACTGATATCCACTCACCCCAAGTCACTGTCAAAGAGTCGCTAATTTACTCAGCTTTCCTTAGGCTCCCTAAAGAAGTCAGCAAACAGGACAAGATGATTTTCGTCCAAGAGGTGATGGAATTGGTCGAGCTAGACAGTCTCAAGGATGCTCTGGTCGGGCTTCCTGGAATTACAGGGTTGTCAACCGAACAAAGAAAGAGGTTAACAATTGCAGTTGAGCTTGTTGCTAATCCCTCAATCATTTTCATGGATGAACCAACATCAGGTCTTGATGCAAGGGCAGCTGCAATTGTTATGAGGACTGTTAGAAATACGGTGGACACTGGGAGAACAGTTGTCTGCACTATTCACCAGCCTAGCATTGATATATTCGAGGCATTTGATGAGCTTCTACTCCTGAAGAGAGGAGGACAAGTTATCTACTCGGGACCATTAGGCCGCAATTCCCACAAGATCATCGAATATTTTGAGGCTATTCCTGGAGTGCAAAAAATAAAAGAAAAGTATAATCCAGCCACATGGATGCTTGAGGTAAGCTCAGTAGGAACTGAGGTCAAGCTTGGAATGGACTTTGCTCAATACTACAGATCATCTTCCTTGCACCAGAGAAACAAAGCTTTAGTAAAGGAGCTAAGCAAACCATCTCCAGGAGCAAAAGATCTCTATTTTGCTACTCAGTATTCACAGTCATCATTACAACAATTCAAATCATGTCTGTGGAAGCAGTGGTGGACTTATTGGAGAACTCCTGATTATAACCTTGTTAGATTCTTTTTCACCTTGGCTTGTGCTCTCATGCTTGGAACTATGTTCTGGAAGGTTGGCACTAAAAGGGAAAGCACTTCTGATCTGACCATGATTATTGGGGCTATGTATTCTGCTGTGCTGTTCGTTGGAATTAACAACTGCTCCACAGTGCAGCCAATAATCGCCACTGAAAGAACGGTATTCTATCGAGAAAGAGCTGCTGGGATGTACTCTGCATTACCTTATGCACTAGCACAGGTAAGTGGCGATATACATAGACATGCTAGAAATCTAGAATCTCTTGGAAGTGAGATATCGATATCCCCACCTTTAGATATATTATTCATCCAATTGTTATATACTTAA

Protein Analysis

1351

Amino Acids

152.22

Weight (kDa)

8.53

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 107 - 158 3e-09 ABC-transporter N-terminal
ABC_tran PF00005 183 - 364 3.6e-14 ABC transporter
ABC2_membrane PF01061 519 - 730 3.6e-54 ABC-2 type transporter
PDR_assoc PF08370 736 - 800 1.5e-26 Plant PDR ABC transporter associated
ABC_tran PF00005 918 - 1069 2.7e-19 ABC transporter
ABC2_membrane_7 PF19055 1098 - 1159 4.3e-06 ABC-2 type transporter
ABC2_membrane PF01061 1214 - 1321 5.6e-27 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3699
Acc36I ACCTGC 3 cut(s) 17, 1204, 1938
AccB1I GGYRCC 1 cut(s) 801
AccB7I CCANNNNNTGG 2 cut(s) 1899, 1990
AccI GTMKAC 2 cut(s) 1257, 1611
AccII CGCG 1 cut(s) 1270
AciI CCGC 4 cut(s) 1268, 1302, 2808, 3382
AclWI GGATC 6 cut(s) 197, 647, 660, 1975, 2009, 2810
AcoI YGGCCR 2 cut(s) 1024, 2412
AcsI RAATTY 6 cut(s) 707, 957, 1424, 2115, 2883, 2922
AcuI CTGAAG 8 cut(s) 141, 287, 1185, 1959, 1990, 2208, 2424, 3362
AfaI GTAC 5 cut(s) 31, 714, 817, 2366, 3920
AfiI CCNNNNNNNGG 8 cut(s) 611, 631, 1240, 1899, 1990, 2633, 3206, 3590
AflIII ACRYGT 1 cut(s) 1749
AgeI ACCGGT 2 cut(s) 1100, 2762
AjnI CCWGG 8 cut(s) 1260, 2201, 2377, 2444, 2776, 3106, 3421, 3589
AjuI GAANNNNNNNTTGG 8 cut(s) 46, 78, 423, 455, 483, 515, 735, 767
Alw21I GWGCWC 6 cut(s) 454, 1158, 1479, 1726, 1773, 3736
Alw26I GTCTC 5 cut(s) 938, 1199, 1809, 2424, 3086
Alw44I GTGCAC 2 cut(s) 1154, 1722
AlwI GGATC 6 cut(s) 197, 647, 660, 1975, 2009, 2810
AlwNI CAGNNNCTG 1 cut(s) 1421
Ama87I CYCGRG 3 cut(s) 875, 1316, 3367
AoxI GGCC 8 cut(s) 1024, 1064, 1504, 1563, 2412, 2468, 2774, 3379
ApaLI GTGCAC 2 cut(s) 1154, 1722
ApoI RAATTY 6 cut(s) 707, 957, 1424, 2115, 2883, 2922
ArsI GACNNNNNNTTYG 2 cut(s) 1651, 1683
AsiGI ACCGGT 2 cut(s) 1100, 2762
Asp700I GAANNNNTTC 1 cut(s) 350
AspLEI GCGC 1 cut(s) 2626
AspS9I GGNCC 6 cut(s) 599, 1064, 1265, 2031, 2469, 3371
AsuC2I CCSGG 1 cut(s) 2890
AsuHPI GGTGA 6 cut(s) 1061, 2142, 2949, 3067, 3284, 3712
AsuII TTCGAA 1 cut(s) 2268
AsuNHI GCTAGC 2 cut(s) 841, 1943
AvaI CYCGRG 3 cut(s) 875, 1316, 3367
AvaII GGWCC 4 cut(s) 599, 1265, 2031, 3371
AxyI CCTNAGG 1 cut(s) 3002
BaeGI GKGCMC 2 cut(s) 1158, 1726
BaeI ACNNNNGTAYC 2 cut(s) 975, 1008
BalI TGGCCA 1 cut(s) 1026
BamHI GGATCC 1 cut(s) 652
BanI GGYRCC 1 cut(s) 801
BanII GRGCYC 4 cut(s) 454, 1479, 1773, 2062
BauI CACGAG 1 cut(s) 1500
BbsI GAAGAC 3 cut(s) 129, 728, 1679
Bbv12I GWGCWC 6 cut(s) 454, 1158, 1479, 1726, 1773, 3736
BccI CCATC 8 cut(s) 577, 583, 1697, 1897, 2416, 2824, 3052, 3592
BceAI ACGGC 1 cut(s) 537
BcgI CGANNNNNNTGC 2 cut(s) 2732, 2766
BciT130I CCWGG 8 cut(s) 1262, 2203, 2379, 2446, 2778, 3108, 3423, 3591
BciVI GTATCC 2 cut(s) 976, 1552
BclI TGATCA 3 cut(s) 409, 1740, 2037
BcnI CCSGG 1 cut(s) 2890
BcoDI GTCTC 5 cut(s) 938, 1199, 1809, 2424, 3086
BfmI CTRYAG 5 cut(s) 2026, 2307, 2352, 2741, 3526
BfuAI ACCTGC 3 cut(s) 17, 1204, 1938
BfuI GTATCC 2 cut(s) 976, 1552
BglII AGATCT 1 cut(s) 3601
BlpI GCTNAGC 2 cut(s) 1767, 3575
Bme1390I CCNGG 9 cut(s) 1262, 2203, 2379, 2446, 2778, 2890, 3108, 3423, 3591
Bme18I GGWCC 4 cut(s) 599, 1265, 2031, 3371
BmeT110I CYCGRG 3 cut(s) 875, 1316, 3367
BmgT120I GGNCC 6 cut(s) 599, 1064, 1265, 2031, 2469, 3371
BmiI GGNNCC 8 cut(s) 601, 654, 803, 1065, 1266, 2894, 3008, 3372
BmrFI CCNGG 9 cut(s) 1262, 2203, 2379, 2446, 2778, 2890, 3108, 3423, 3591
BmrI ACTGGG 2 cut(s) 2152, 3276
BmsI GCATC 7 cut(s) 234, 307, 1301, 2506, 3079, 3205, 3456
BmtI GCTAGC 2 cut(s) 845, 1947
BmuI ACTGGG 2 cut(s) 2152, 3276
BpiI GAAGAC 3 cut(s) 129, 728, 1679
BplI GAGNNNNNCTC 6 cut(s) 571, 603, 1340, 1372, 3464, 3496
BpmI CTGGAG 3 cut(s) 2040, 3444, 3573
Bpu1102I GCTNAGC 2 cut(s) 1767, 3575
Bpu14I TTCGAA 1 cut(s) 2268
BpuEI CTTGAG 3 cut(s) 533, 3068, 3491
BpuMI CCSGG 1 cut(s) 2890
Bsa29I ATCGAT 1 cut(s) 4007
BsaBI GATNNNNATC 1 cut(s) 1411
BsaI GGTCTC 2 cut(s) 1809, 2424
BsaJI CCNNGG 7 cut(s) 805, 876, 1261, 2201, 2445, 2777, 3723
BsaWI WCCGGW 2 cut(s) 1100, 2762
BsaXI ACNNNNNCTCC 6 cut(s) 65, 95, 3342, 3372, 3836, 3866
Bsc4I CCNNNNNNNGG 8 cut(s) 611, 631, 1240, 1899, 1990, 2633, 3206, 3590
Bse118I RCCGGY 2 cut(s) 1100, 2762
Bse1I ACTGG 9 cut(s) 196, 565, 2056, 2147, 2294, 2571, 2613, 2860, 3271
Bse21I CCTNAGG 1 cut(s) 3002
Bse3DI GCAATG 4 cut(s) 483, 918, 1782, 2670
Bse8I GATNNNNATC 1 cut(s) 1411
BseBI CCWGG 8 cut(s) 1262, 2203, 2379, 2446, 2778, 3108, 3423, 3591
BseCI ATCGAT 1 cut(s) 4007
BseDI CCNNGG 7 cut(s) 805, 876, 1261, 2201, 2445, 2777, 3723
BseJI GATNNNNATC 1 cut(s) 1411
BseLI CCNNNNNNNGG 8 cut(s) 611, 631, 1240, 1899, 1990, 2633, 3206, 3590
BseMI GCAATG 4 cut(s) 483, 918, 1782, 2670
BseMII CTCAG 9 cut(s) 828, 1194, 1758, 2383, 2510, 3006, 3483, 3495, 3632
BseNI ACTGG 9 cut(s) 196, 565, 2056, 2147, 2294, 2571, 2613, 2860, 3271
BseRI GAGGAG 3 cut(s) 933, 2646, 3363
BseSI GKGCMC 2 cut(s) 1158, 1726
BseYI CCCAGC 3 cut(s) 641, 1997, 3911
BsgI GTGCAG 2 cut(s) 3268, 3881
Bsh1236I CGCG 1 cut(s) 1270
Bsh1285I CGRYCG 2 cut(s) 286, 423
BshFI GGCC 8 cut(s) 1026, 1066, 1506, 1565, 2414, 2470, 2776, 3381
BshNI GGYRCC 1 cut(s) 801
BshTI ACCGGT 2 cut(s) 1100, 2762
BshVI ATCGAT 1 cut(s) 4007
BsiEI CGRYCG 2 cut(s) 286, 423
BsiHKAI GWGCWC 6 cut(s) 454, 1158, 1479, 1726, 1773, 3736
BsiHKCI CYCGRG 3 cut(s) 875, 1316, 3367
BsiSI CCGG 4 cut(s) 1101, 2545, 2763, 2889
BslFI GGGAC 4 cut(s) 585, 1278, 1586, 3384
BslI CCNNNNNNNGG 8 cut(s) 611, 631, 1240, 1899, 1990, 2633, 3206, 3590
BsmAI GTCTC 5 cut(s) 938, 1199, 1809, 2424, 3086
BsmBI CGTCTC 1 cut(s) 1199
BsmFI GGGAC 4 cut(s) 585, 1278, 1586, 3384
BsmI GAATGC 3 cut(s) 437, 559, 2593
BsnI GGCC 8 cut(s) 1026, 1066, 1506, 1565, 2414, 2470, 2776, 3381
Bso31I GGTCTC 2 cut(s) 1809, 2424
BsoBI CYCGRG 3 cut(s) 875, 1316, 3367
Bsp119I TTCGAA 1 cut(s) 2268
Bsp1286I GDGCHC 7 cut(s) 454, 1158, 1479, 1726, 1773, 2062, 3736
Bsp1720I GCTNAGC 2 cut(s) 1767, 3575
BspACI CCGC 4 cut(s) 1268, 1302, 2808, 3382
BspANI GGCC 8 cut(s) 1026, 1066, 1506, 1565, 2414, 2470, 2776, 3381
BspCNI CTCAG 9 cut(s) 829, 1195, 1759, 2382, 2509, 3005, 3484, 3494, 3631
BspDI ATCGAT 1 cut(s) 4007
BspFNI CGCG 1 cut(s) 1270
BspHI TCATGA 1 cut(s) 897
BspLI GGNNCC 8 cut(s) 601, 654, 803, 1065, 1266, 2894, 3008, 3372
BspMAI CTGCAG 3 cut(s) 2030, 2311, 2745
BspMI ACCTGC 3 cut(s) 17, 1204, 1938
BspOI GCTAGC 2 cut(s) 845, 1947
BspPI GGATC 6 cut(s) 197, 647, 660, 1975, 2009, 2810
BspQI GCTCTTC 2 cut(s) 459, 2319
BspT104I TTCGAA 1 cut(s) 2268
BspT107I GGYRCC 1 cut(s) 801
BspTNI GGTCTC 2 cut(s) 1809, 2424
BsrDI GCAATG 4 cut(s) 483, 918, 1782, 2670
BsrFI RCCGGY 2 cut(s) 1100, 2762
BsrI ACTGG 9 cut(s) 196, 565, 2056, 2147, 2294, 2571, 2613, 2860, 3271
BssAI RCCGGY 2 cut(s) 1100, 2762
BssECI CCNNGG 7 cut(s) 805, 876, 1261, 2201, 2445, 2777, 3723
BssSI CACGAG 1 cut(s) 1500
BssT1I CCWWGG 2 cut(s) 805, 3723
Bst2BI CACGAG 1 cut(s) 1500
Bst2UI CCWGG 8 cut(s) 1262, 2203, 2379, 2446, 2778, 3108, 3423, 3591
Bst6I CTCTTC 7 cut(s) 123, 459, 1223, 2319, 2339, 2412, 3338
BstAPI GCANNNNNTGC 2 cut(s) 1153, 1966
BstBI TTCGAA 1 cut(s) 2268
BstC8I GCNNGC 4 cut(s) 843, 1142, 1397, 1945
BstEII GGTNACC 1 cut(s) 1351
BstFNI CGCG 1 cut(s) 1270
BstHHI GCGC 1 cut(s) 2626
BstMAI GTCTC 5 cut(s) 938, 1199, 1809, 2424, 3086
BstMCI CGRYCG 2 cut(s) 286, 423
BstMWI GCNNNNNNNGC 5 cut(s) 494, 1153, 1940, 1966, 3224
BstNI CCWGG 8 cut(s) 1262, 2203, 2379, 2446, 2778, 3108, 3423, 3591
BstNSI RCATGY 2 cut(s) 2701, 3974
BstPI GGTNACC 1 cut(s) 1351
BstSCI CCNGG 9 cut(s) 1260, 2201, 2377, 2444, 2776, 2888, 3106, 3421, 3589
BstSFI CTRYAG 5 cut(s) 2026, 2307, 2352, 2741, 3526
BstSLI GKGCMC 2 cut(s) 1158, 1726
BstUI CGCG 1 cut(s) 1270
BstV2I GAAGAC 3 cut(s) 129, 728, 1679
BstX2I RGATCY 3 cut(s) 652, 2001, 3601
BstXI CCANNNNNNTGG 3 cut(s) 812, 2127, 3462
BstYI RGATCY 3 cut(s) 652, 2001, 3601
Bsu15I ATCGAT 1 cut(s) 4007
Bsu36I CCTNAGG 1 cut(s) 3002
BsuI GTATCC 2 cut(s) 976, 1552
BsuRI GGCC 8 cut(s) 1026, 1066, 1506, 1565, 2414, 2470, 2776, 3381
BsuTUI ATCGAT 1 cut(s) 4007
BtsI GCAGTG 3 cut(s) 2138, 2259, 3674
BveI ACCTGC 3 cut(s) 17, 1204, 1938
Cac8I GCNNGC 4 cut(s) 843, 1142, 1397, 1945
CaiI CAGNNNCTG 1 cut(s) 1421
CciI TCATGA 1 cut(s) 897
CfoI GCGC 1 cut(s) 2626
Cfr10I RCCGGY 2 cut(s) 1100, 2762
Cfr13I GGNCC 6 cut(s) 599, 1064, 1265, 2031, 2469, 3371
ClaI ATCGAT 1 cut(s) 4007
Csp6I GTAC 5 cut(s) 30, 713, 816, 2365, 3919
CspAI ACCGGT 2 cut(s) 1100, 2762
CspCI CAANNNNNGTGG 4 cut(s) 1817, 1852, 2554, 2589
CviQI GTAC 5 cut(s) 30, 713, 816, 2365, 3919
DraI TTTAAA 1 cut(s) 1441
EaeI YGGCCR 2 cut(s) 1024, 2412
Eam1104I CTCTTC 7 cut(s) 123, 459, 1223, 2319, 2339, 2412, 3338
EarI CTCTTC 7 cut(s) 123, 459, 1223, 2319, 2339, 2412, 3338
Ecl136II GAGCTC 3 cut(s) 452, 1477, 1771
Eco130I CCWWGG 2 cut(s) 805, 3723
Eco147I AGGCCT 2 cut(s) 1565, 2776
Eco24I GRGCYC 4 cut(s) 454, 1479, 1773, 2062
Eco31I GGTCTC 2 cut(s) 1809, 2424
Eco32I GATATC 4 cut(s) 1228, 2950, 4005, 4011
Eco47I GGWCC 4 cut(s) 599, 1265, 2031, 3371
Eco53kI GAGCTC 3 cut(s) 452, 1477, 1771
Eco57I CTGAAG 8 cut(s) 141, 287, 1185, 1959, 1990, 2208, 2424, 3362
Eco81I CCTNAGG 1 cut(s) 3002
Eco88I CYCGRG 3 cut(s) 875, 1316, 3367
Eco91I GGTNACC 1 cut(s) 1351
EcoICRI GAGCTC 3 cut(s) 452, 1477, 1771
EcoO109I RGGNCCY 2 cut(s) 599, 1064
EcoO65I GGTNACC 1 cut(s) 1351
EcoRI GAATTC 1 cut(s) 1424
EcoRII CCWGG 8 cut(s) 1260, 2201, 2377, 2444, 2776, 3106, 3421, 3589
EcoRV GATATC 4 cut(s) 1228, 2950, 4005, 4011
EcoT14I CCWWGG 2 cut(s) 805, 3723
EcoT38I GRGCYC 4 cut(s) 454, 1479, 1773, 2062
ErhI CCWWGG 2 cut(s) 805, 3723
Esp3I CGTCTC 1 cut(s) 1199
FalI AAGNNNNNCTT 2 cut(s) 876, 908
FaqI GGGAC 4 cut(s) 585, 1278, 1586, 3384
FauI CCCGC 1 cut(s) 2801
FauNDI CATATG 1 cut(s) 169
FbaI TGATCA 3 cut(s) 409, 1740, 2037
FblI GTMKAC 2 cut(s) 1257, 1611
FriOI GRGCYC 4 cut(s) 454, 1479, 1773, 2062
GlaI GCGC 1 cut(s) 2625
GsaI CCCAGC 3 cut(s) 645, 2001, 3915
GsuI CTGGAG 3 cut(s) 2040, 3444, 3573
HaeIII GGCC 8 cut(s) 1026, 1066, 1506, 1565, 2414, 2470, 2776, 3381
HapII CCGG 4 cut(s) 1101, 2545, 2763, 2889
HhaI GCGC 1 cut(s) 2626
Hin6I GCGC 1 cut(s) 2624
HinP1I GCGC 1 cut(s) 2624
HincII GTYRAC 3 cut(s) 889, 3126, 3147
HindII GTYRAC 3 cut(s) 889, 3126, 3147
HindIII AAGCTT 3 cut(s) 269, 3500, 3558
HpaI GTTAAC 1 cut(s) 3147
HpaII CCGG 4 cut(s) 1101, 2545, 2763, 2889
HphI GGTGA 6 cut(s) 1061, 2142, 2949, 3067, 3284, 3712
Hpy99I CGWCG 1 cut(s) 610
HpyAV CCTTC 9 cut(s) 219, 635, 843, 895, 1829, 2460, 2714, 2864, 3754
HpyCH4IV ACGT 2 cut(s) 1208, 1751
HpyF10VI GCNNNNNNNGC 5 cut(s) 494, 1153, 1940, 1966, 3224
HpySE526I ACGT 2 cut(s) 1208, 1751
HspAI GCGC 1 cut(s) 2624
Ksp22I TGATCA 3 cut(s) 409, 1740, 2037
KspAI GTTAAC 1 cut(s) 3147
LguI GCTCTTC 2 cut(s) 459, 2319
LmnI GCTCC 7 cut(s) 1118, 1204, 1706, 3012, 3571, 3593, 3857
LweI GCATC 7 cut(s) 234, 307, 1301, 2506, 3079, 3205, 3456
MaeII ACGT 2 cut(s) 1208, 1751
MfeI CAATTG 3 cut(s) 3150, 3231, 4040
MflI RGATCY 3 cut(s) 652, 2001, 3601
MhlI GDGCHC 7 cut(s) 454, 1158, 1479, 1726, 1773, 2062, 3736
MlsI TGGCCA 1 cut(s) 1026
MluNI TGGCCA 1 cut(s) 1026
MlyI GAGTC 2 cut(s) 2031, 2987
MmeI TCCRAC 6 cut(s) 44, 630, 973, 1216, 2008, 3817
Mox20I TGGCCA 1 cut(s) 1026
MroXI GAANNNNTTC 1 cut(s) 350
MscI TGGCCA 1 cut(s) 1026
MseI TTAA 8 cut(s) 669, 1440, 1620, 1746, 2682, 3146, 3843, 4054
MslI CAYNNNNRTG 5 cut(s) 1415, 3188, 3464, 3658, 3969
Msp20I TGGCCA 1 cut(s) 1026
MspA1I CMGCKG 2 cut(s) 2808, 3227
MspI CCGG 4 cut(s) 1101, 2545, 2763, 2889
MspR9I CCNGG 9 cut(s) 1262, 2203, 2379, 2446, 2778, 2890, 3108, 3423, 3591
MunI CAATTG 3 cut(s) 3150, 3231, 4040
Mva1269I GAATGC 3 cut(s) 437, 559, 2593
MvaI CCWGG 8 cut(s) 1262, 2203, 2379, 2446, 2778, 3108, 3423, 3591
MvnI CGCG 1 cut(s) 1270
MwoI GCNNNNNNNGC 5 cut(s) 494, 1153, 1940, 1966, 3224
NciI CCSGG 1 cut(s) 2890
NdeI CATATG 1 cut(s) 169
NheI GCTAGC 2 cut(s) 841, 1943
NlaIV GGNNCC 8 cut(s) 601, 654, 803, 1065, 1266, 2894, 3008, 3372
NmuCI GTSAC 3 cut(s) 1417, 1497, 2965
NspI RCATGY 2 cut(s) 2701, 3974
NspV TTCGAA 1 cut(s) 2268
PagI TCATGA 1 cut(s) 897
PceI AGGCCT 2 cut(s) 1565, 2776
PciSI GCTCTTC 2 cut(s) 459, 2319
PctI GAATGC 3 cut(s) 437, 559, 2593
PdmI GAANNNNTTC 1 cut(s) 350
PfeI GAWTC 8 cut(s) 217, 491, 1889, 2441, 2486, 2594, 3711, 3986
PflMI CCANNNNNTGG 2 cut(s) 1899, 1990
PfoI TCCNGGA 4 cut(s) 2377, 3106, 3421, 3589
PinAI ACCGGT 2 cut(s) 1100, 2762
PleI GAGTC 2 cut(s) 2030, 2986
PpsI GAGTC 2 cut(s) 2030, 2986
PpuMI RGGWCCY 1 cut(s) 599
PsiI TTATAA 1 cut(s) 3699
Psp124BI GAGCTC 3 cut(s) 454, 1479, 1773
Psp5II RGGWCCY 1 cut(s) 599
Psp6I CCWGG 8 cut(s) 1260, 2201, 2377, 2444, 2776, 3106, 3421, 3589
PspEI GGTNACC 1 cut(s) 1351
PspFI CCCAGC 3 cut(s) 641, 1997, 3911
PspGI CCWGG 8 cut(s) 1260, 2201, 2377, 2444, 2776, 3106, 3421, 3589
PspN4I GGNNCC 8 cut(s) 601, 654, 803, 1065, 1266, 2894, 3008, 3372
PspPI GGNCC 6 cut(s) 599, 1064, 1265, 2031, 2469, 3371
PspPPI RGGWCCY 1 cut(s) 599
PstI CTGCAG 3 cut(s) 2030, 2311, 2745
PstNI CAGNNNCTG 1 cut(s) 1421
PsuI RGATCY 3 cut(s) 652, 2001, 3601
PvuII CAGCTG 1 cut(s) 3227
RsaI GTAC 5 cut(s) 31, 714, 817, 2366, 3920
RsaNI GTAC 5 cut(s) 30, 713, 816, 2365, 3919
RseI CAYNNNNRTG 5 cut(s) 1415, 3188, 3464, 3658, 3969
SacI GAGCTC 3 cut(s) 454, 1479, 1773
SapI GCTCTTC 2 cut(s) 459, 2319
SaqAI TTAA 8 cut(s) 669, 1440, 1620, 1746, 2682, 3146, 3843, 4054
Sau96I GGNCC 6 cut(s) 599, 1064, 1265, 2031, 2469, 3371
SchI GAGTC 2 cut(s) 2031, 2987
ScrFI CCNGG 9 cut(s) 1262, 2203, 2379, 2446, 2778, 2890, 3108, 3423, 3591
SduI GDGCHC 7 cut(s) 454, 1158, 1479, 1726, 1773, 2062, 3736
SfaNI GCATC 7 cut(s) 234, 307, 1301, 2506, 3079, 3205, 3456
SfcI CTRYAG 5 cut(s) 2026, 2307, 2352, 2741, 3526
SfuI TTCGAA 1 cut(s) 2268
SinI GGWCC 4 cut(s) 599, 1265, 2031, 3371
SmiMI CAYNNNNRTG 5 cut(s) 1415, 3188, 3464, 3658, 3969
SmlI CTYRAG 3 cut(s) 548, 3083, 3470
SmoI CTYRAG 3 cut(s) 548, 3083, 3470
SseBI AGGCCT 2 cut(s) 1565, 2776
SsiI CCGC 4 cut(s) 1268, 1302, 2808, 3382
SspI AATATT 3 cut(s) 870, 1379, 3407
SstI GAGCTC 3 cut(s) 454, 1479, 1773
StuI AGGCCT 2 cut(s) 1565, 2776
StyD4I CCNGG 9 cut(s) 1260, 2201, 2377, 2444, 2776, 2888, 3106, 3421, 3589
StyI CCWWGG 2 cut(s) 805, 3723
TaiI ACGT 2 cut(s) 1211, 1754
TatI WGTACW 1 cut(s) 3918
TauI GCSGC 1 cut(s) 3384
TfiI GAWTC 8 cut(s) 217, 491, 1889, 2441, 2486, 2594, 3711, 3986
Tru1I TTAA 8 cut(s) 669, 1440, 1620, 1746, 2682, 3146, 3843, 4054
Tru9I TTAA 8 cut(s) 669, 1440, 1620, 1746, 2682, 3146, 3843, 4054
TseFI GTSAC 3 cut(s) 1417, 1497, 2965
Tsp45I GTSAC 3 cut(s) 1417, 1497, 2965
Van91I CCANNNNNTGG 2 cut(s) 1899, 1990
VneI GTGCAC 2 cut(s) 1154, 1722
VpaK11BI GGWCC 4 cut(s) 599, 1265, 2031, 3371
XapI RAATTY 6 cut(s) 707, 957, 1424, 2115, 2883, 2922
XbaI TCTAGA 3 cut(s) 1105, 1358, 3982
XceI RCATGY 2 cut(s) 2701, 3974
XcmI CCANNNNNNNNNTGG 3 cut(s) 1900, 2245, 2635
XmiI GTMKAC 2 cut(s) 1257, 1611
XmnI GAANNNNTTC 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.