MD09G1203500.v1.1
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002725
Physical Location & Seq
Reverse (-)
19104442 .. 19114708
10267 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1203500.v1.1.491

Sequence Viewer

Length: 4557 bp
ATGTTTAAATATCCACCAACTTATCCAAGGAGAGCCGAGCAAACTCTTGCACGGTTGCACCAGTGGGGACTTGGGAAGAAAATGGAAGGAACAGAAAAAGTGAGAGGTTCGCCGCTAAGGCACCACAGCTTTAGCAGGAGCAGAAGCAAAGGGTTAAGCATGGAGGACGTGTTTTCAAGTGCCACTCACTCTCGACGAGGCAGTCGAAGAGACGAAGACGAAGAAGCTCTGACATGGGCTGCCATTGAAAAATTGCCAACATATAATCGGCTGAGAACAAGCATCATCAAGTCCTTTGTCGAAGGCGATGTTCAAGGAAATAACAACAACAAAGTGGTGCATAAAGAAGTGGACGTTCTAAAGCTCGACCTGATCGACCGTCAGAAATTCATTGACAGCACTTTTAAGGTTGCAGAGGAAGATAATGAGAGGTTCTTGAAGAAGTTTAGAAGTAGAATAGACAAGGTTGGGATCAAACTTCCAACAGTTGAAGTTAGGTTCCAGCATTTGGCAGTCGAAGCCGACTGCCACATCGGCACCAGAGCTCTTCCCAGTCTTCCAAATGTTGCTCGAAACATCGCAGAATCAGCACTTGGTTTAATTGGGATCCAAATGGCTAAGAGAACAAATCTCACTATTCTCAAGGATGCCTCTGGCATTGTTAAACCATCAAGGATGACTCTTTTATTAGCTCCGCCAGCGTCAGGGAAAACAACCCTTTTGCTTGCACTAGCTGGGAAGTTAGACCCAAGTTTGAAGGTTAGAGGAGAAATCACTTACAATGGATACAGGCTGAATGAATTTGTGCCACAAAAGACATCTGCATACATAAGCCAAAATGATGTTCATGCAGGAGAATTGACAGTCAAAGAAACTCTAGACTTTTCGGCAAGGTGCCAAGGGGTTGGGACACGATATGAACTTCTTTCCGAGCTTGCTAGAAGAGAAAAGGAGGCTGGAATATTTCCTGAGGCAGATATTGACCTTTTCATGAAGGCTACGTCAATGGGAGGAGTTGAGAGCAGTCTCATTACTGACTATACTCTCAAAATTTTGGGGCTTGATATATGCAAGGACACCATTGTAGGAAATCAGATGCGGAGAGGGATATCTGGTGGACAGAAAAAACGAGTTACCACAGGTGAGATGATCGTTGGGCCTACAAAAACATTATTCATGGATGAGATATCAACAGGTCTTGATAGCTCCACAACACATCAAATAGTGAAGTGCTTGCAACAAATTGTACATATCACCGAGGCCACGATCTTGATGTCCCTACTGCAACCTGCTCCTGAGACGTTTGATCTCTTTGATGATATCATCCTTTTATCGGAGGGCAAGATCATCTACCAAGGACCACGCAAGCATGTACTGGAGTTCTTTGAGAGTTGCGGATTTCGGTGCCCTAGGCGAAAGGGAACTGCTGATTTCTTGCAAGAGGTTACCTCAAGGAAAGACCAAGAGCAATATTGGGCAGACAGAAACAAGCCATACCGACACATATCAGTCACTGAATTTGCGAACCGGTTCAAGCGCTTCCATGTGGGACTGTGTCTCCAGAATGAGCTCTCAACCCCTTACAATAAGGCCCGAGGCCATGGGGCAGCCCTTGTTTTCACAAGATATTCAATTTCCAAAATGGATCTTCTAAAAGCCTGTTTTGACAAGGAGTGGCTACTAATAAAGAGGAATTCTCTTGTTTACATTTTCAAGACAGTCAAAAGTATTATAGCAGCATTAGTAGTCACGACCGTATTCTTGAGGACGCAAATGCACTCTAGAAATGAAAATGATGGTGCGCTATATATTGGTGCACTTATATTTTCCATGATCATTAACATGTTCAATGGCATGGCTGAGCTCTCATTGACCATTGCAAAACTCCCCGTGTTTTACAAACATAGAGACCTTCTTTTCCACCCTGCTTGGACTTTCACTCTCCCTAATGTCCTACTCCGGATTCCTGTATCCCTCTTTGAATCCCTTGTTTGGGTGGCCATTACGTACTACACCATAGGATATGCACCTGAGATCAGCAGGTTTCTCAAGCAACTATTGCTGCTATTTCTGCTCCAACAAATGGCTACAGGTATGTTTTGGCTTATTGCTGGAGTGTGCAGGTCCATGATCATTGCCAACACTGGCGGGTCTCTAGCTCTGCTCACTGTTTCAATGCTTGGGGGTTTCATAATTCGTCGAGATGAAATTCCAAAGTGGTGGCGGTGGGGCTACTGGGCTTCACCTATAACATATGGTTTCAATGCTATTACTGTAAACGAAATGTTTGCTCCGAGGTGGATGAACAAACTGGCTTCAGATAATGCTACAAGATTAGGTGTAGCAGTGCTTGAAATATTTGGTGTTTTCCCAGATAAAAACTGGTTTTGGATTGGCAGTTTAGCTGTTTTGGGGTTTGCAGTTCTCTTCAACATCCTTTTCCCACTAGTGCTTATGTACCTGAATCCACTTGGAAGGCCACAAGCGGTAATATCTGAAGAAGTGGCAGAGGAAAGAGAAGAACCAAGACTTAGAAGACCCAAATCAAAGAATTCACTTTCACGATCACTATCATTTTCTCGATCATTAACTTCTTTGGATGGAAACAATACAAGAGAAATGGAAATCCAGCGAATGAGCAGTCGATCGAATTCCAATGGAATAAGTAGAAATGCTGATTCGTCTCTTGAAGCAGCCGGTGTTGCCCCCAAGAGAGGAATGGTTCTTCCCTTCACTCCCCTTGGAATGTCCTTTGACAGTGTTAACTACTATGTAGACATGCCCCCTCAAATGAAGGAAGAAGGAGTAAAAGAGGACAGGCTTCAGCTGCTTTGTGAAGTAACTGGTTCATTTAGGCCTGGGGTCTTGACAGCTCTAATGGGAGTCAGTGGAGCAGGGAAGACAACTTTGATGGATGTCTTAGCGGGAAGAAAGACAGGTGGTTACATTGAAGGCGATATTAGAATTTCAGGGTACCCTAAGAAACAAGAAACCTTTGCCAGAATTTTTGGTTATTGTGAACAAAATGATATCCACTCCCCCCAAGTCACAGTCAAAGAATCATTGATTTATTCAGCTTTCCTTAGGCTCCCAAAAGAAGTCAGCAAAACTGAAAAGATGATATTTTTAGAAGAAGTGATGAACTTTGTGGAGCTAGACAATCTCAAGGATGCCATCGTGGGGCTTCCAGGAATTACAGGGTTGTCAACAGAACAAAGAAAAAGGTTAACAATTGCAGTTGAGCTTGTTGCCAATCCCTCAATCATATTCATGGATGAACCAACATCAGGTCTTGATGCAAGGGCCGCTGCCATTGTTATGAGGACGGTTAGGAATACTGTAGACACTGGTAGAACAGTTGTCTGCACAATTCATCAGCCTAGCATAGATATCTTTGAGGCGTTCGATGAGCTGCAACTCATGAAGAGAGGAGGACAAGTGATCTACTCTGGACCTTTGGGCCGTAATTCTCACAAGATCATTGAGTATTTTGAGGCAGTTCCAGGAGTGCCAAAAATTAAAGAGAGGTCTAATCCTGCTACATGGATGCTAGAGGTGAGCTCTGTAGCGACTGAATTCAAACTTGGAATGGACTTCGCTCAACACTACAAATCATCTTCCTTGCATCAGAGAAACAAAGCTTTAATAAATGAGTTAAGTACACCACCACCGGGAGCAAAAGACCTGCATTTTCCTACTCAGTATTCTCAATCGACATGGGAGCAATTCAAATCTTGCCTGTGGAAGCAGTGGTGGACTTATTGGAGGTGTCCCGATTACAACCTTGTTAGGTTCTTTTTTACCATGGTCGCCTCCCTCCTGCTCGGAACTATGTTCTGGAAGATTGGCACTAAAAGGGAAAGCGTAGCTGATCTGACCATGATTATCGGAGCTATGAATTCTGCTATAATTTTTATTGGAGTTAATAACGGCTCAACAGTGCAGCCAATGGTCGCTGTTGAAAGAACGGTATTCTATCGAGAAAGAGCTGCTGGGATGTACTCTGCATTACCTTATGCACTGGCGCAAGTTATTGTTGAAATACCGTATGTATTTATTCAAACTTCATATTATGTGTTGATTGTGTATGCTATGATGAGCTTCCAATGGACAGTAGCAAAATTCTTCTGGTTCTTCTTTGTCAGCTTCTTCTCATTCCTTTATTTCACATACTACGGAATGATGACTGTTTCCATCACACCAAATATCCAAGTAGCGGGCATAGTGGCATCGTTCTTCTATTCGTTCTTCAATCTTTTCTCGGGTTTCTACATACCAAAACCAAAAATACCCAAATGGTGGATTTGGTTTTACTATATATGCCCAGTATCATGGACAGTGTATGGATTCATTGTGTCACAGTTTGGCGATGTGGAGGACACCGTTAGAGCACCTGGGATTACACCTGATCCGACCATCAAATGGTACATCGAAAACCATTTTGGATATGACACGAATTTCATGGGACCAGTGGCAGTGATTCTAGTTGGCTTCGCAGCTTTCTTTGCCTTTATGTATGCCTTTGGCATTAGGGCACTCAACTTCCAGATTAGATAG

Protein Analysis

1519

Amino Acids

171.95

Weight (kDa)

9.25

Isoelectric Point (pI)

40.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 139 - 189 1.2e-08 ABC-transporter N-terminal
ABC_tran PF00005 214 - 395 6.4e-12 ABC transporter
ABC2_membrane PF01061 551 - 761 5.6e-53 ABC-2 type transporter
PDR_assoc PF08370 767 - 831 2.9e-24 Plant PDR ABC transporter associated
ABC_tran PF00005 947 - 1096 3.9e-20 ABC transporter
ABC2_membrane PF01061 1241 - 1455 1.1e-58 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 201
Acc36I ACCTGC 4 cut(s) 1297, 2031, 2112, 3693
Acc65I GGTACC 1 cut(s) 2973
AccB1I GGYRCC 5 cut(s) 120, 536, 894, 1404, 2973
AccB7I CCANNNNNTGG 4 cut(s) 508, 2083, 4299, 4422
AccI GTMKAC 2 cut(s) 2775, 3342
AccIII TCCGGA 1 cut(s) 1959
AclWI GGATC 5 cut(s) 479, 601, 614, 1653, 4403
AcoI YGGCCR 1 cut(s) 1998
AcuI CTGAAG 3 cut(s) 2301, 2517, 2807
AfaI GTAC 8 cut(s) 1248, 1374, 2009, 2459, 2975, 3661, 4001, 4427
AfeI AGCGCT 1 cut(s) 1538
AflIII ACRYGT 2 cut(s) 168, 1842
AgeI ACCGGT 1 cut(s) 1527
AhlI ACTAGT 1 cut(s) 2446
AjiI CACGTC 1 cut(s) 169
AjnI CCWGG 4 cut(s) 2857, 3187, 3502, 4393
AjuI GAANNNNNNNTTGG 8 cut(s) 576, 608, 828, 860, 3567, 3599, 4425, 4457
Alw21I GWGCWC 6 cut(s) 547, 1572, 1819, 1866, 3563, 4393
Alw26I GTCTC 7 cut(s) 204, 1031, 1292, 1562, 1902, 2157, 2688
Alw44I GTGCAC 1 cut(s) 1815
AlwI GGATC 5 cut(s) 479, 601, 614, 1653, 4403
AlwNI CAGNNNCTG 1 cut(s) 1514
Ama87I CYCGRG 2 cut(s) 1593, 4261
Aor13HI TCCGGA 1 cut(s) 1959
Aor51HI AGCGCT 1 cut(s) 1538
AoxI GGCC 9 cut(s) 1157, 1260, 1590, 1597, 1998, 2477, 2855, 3303, 3460
ApaLI GTGCAC 1 cut(s) 1815
ArsI GACNNNNNNTTYG 2 cut(s) 2626, 2658
AsiGI ACCGGT 1 cut(s) 1527
Asp700I GAANNNNTTC 1 cut(s) 1529
Asp718I GGTACC 1 cut(s) 2973
AspA2I CCTAGG 1 cut(s) 1409
AspLEI GCGC 3 cut(s) 1539, 1804, 4027
AspS9I GGNCC 8 cut(s) 1157, 1358, 1591, 2124, 3303, 3452, 3460, 4466
AsuC2I CCSGG 1 cut(s) 3672
AsuHPI GGTGA 4 cut(s) 1154, 1246, 2235, 3568
AvaI CYCGRG 2 cut(s) 1593, 4261
AvaII GGWCC 4 cut(s) 1358, 2124, 3452, 4466
AvrII CCTAGG 1 cut(s) 1409
AxyI CCTNAGG 2 cut(s) 969, 3083
BaeGI GKGCMC 3 cut(s) 1409, 1819, 4537
BalI TGGCCA 1 cut(s) 2000
BamHI GGATCC 1 cut(s) 606
BanI GGYRCC 5 cut(s) 120, 536, 894, 1404, 2973
BanII GRGCYC 4 cut(s) 547, 1572, 1866, 3563
BbsI GAAGAC 4 cut(s) 222, 548, 2542, 2906
Bbv12I GWGCWC 6 cut(s) 547, 1572, 1819, 1866, 3563, 4393
BccI CCATC 7 cut(s) 676, 1790, 2594, 2905, 3182, 4202, 4424
BceAI ACGGC 2 cut(s) 3447, 3946
BcgI CGANNNNNNTGC 2 cut(s) 2270, 2304
BciT130I CCWGG 4 cut(s) 2859, 3189, 3504, 4395
BciVI GTATCC 2 cut(s) 779, 1981
BclI TGATCA 2 cut(s) 1833, 2130
BcnI CCSGG 1 cut(s) 3672
BcoDI GTCTC 7 cut(s) 204, 1031, 1292, 1562, 1902, 2157, 2688
BcuI ACTAGT 1 cut(s) 2446
BfmI CTRYAG 3 cut(s) 2088, 3339, 3564
BfoI RGCGCY 1 cut(s) 1540
BfuAI ACCTGC 4 cut(s) 1297, 2031, 2112, 3693
BfuI GTATCC 2 cut(s) 779, 1981
BglI GCCNNNNNGGC 2 cut(s) 118, 534
BlnI CCTAGG 1 cut(s) 1409
BlpI GCTNAGC 1 cut(s) 1860
Bme1390I CCNGG 5 cut(s) 2859, 3189, 3504, 3672, 4395
Bme18I GGWCC 4 cut(s) 1358, 2124, 3452, 4466
BmeT110I CYCGRG 2 cut(s) 1593, 4261
BmgBI CACGTC 1 cut(s) 169
BmgT120I GGNCC 8 cut(s) 1157, 1358, 1591, 2124, 3303, 3452, 3460, 4466
BmiI GGNNCC 9 cut(s) 122, 500, 538, 608, 896, 1406, 2975, 3089, 4467
BmrFI CCNGG 5 cut(s) 2859, 3189, 3504, 3672, 4395
BmrI ACTGGG 3 cut(s) 546, 2245, 4319
BmsI GCATC 8 cut(s) 291, 637, 1086, 3160, 3286, 3537, 3634, 4238
BmuI ACTGGG 3 cut(s) 546, 2245, 4319
BpiI GAAGAC 4 cut(s) 222, 548, 2542, 2906
BplI GAGNNNNNCTC 6 cut(s) 1433, 1465, 1681, 1713, 3545, 3577
BpmI CTGGAG 3 cut(s) 1397, 1544, 2133
Bpu10I CCTNAGC 1 cut(s) 116
Bpu1102I GCTNAGC 1 cut(s) 1860
BpuEI CTTGAG 5 cut(s) 626, 1435, 1783, 2033, 3149
BpuMI CCSGG 1 cut(s) 3672
BsaAI YACGTR 1 cut(s) 2007
BsaBI GATNNNNATC 1 cut(s) 2569
BsaI GGTCTC 2 cut(s) 1902, 2157
BsaWI WCCGGW 2 cut(s) 1527, 1959
BsaXI ACNNNNNCTCC 6 cut(s) 155, 185, 1542, 1572, 3423, 3453
Bse118I RCCGGY 2 cut(s) 1527, 2696
Bse21I CCTNAGG 2 cut(s) 969, 3083
Bse3DI GCAATG 2 cut(s) 1875, 2133
Bse8I GATNNNNATC 1 cut(s) 2569
BseAI TCCGGA 1 cut(s) 1959
BseBI CCWGG 4 cut(s) 2859, 3189, 3504, 4395
BseJI GATNNNNATC 1 cut(s) 2569
BseMI GCAATG 2 cut(s) 1875, 2133
BseMII CTCAG 6 cut(s) 263, 960, 1287, 1851, 2022, 3713
BseRI GAGGAG 3 cut(s) 780, 1026, 3444
BseSI GKGCMC 3 cut(s) 1409, 1819, 4537
BseYI CCCAGC 2 cut(s) 734, 3992
BsgI GTGCAG 3 cut(s) 2140, 3349, 3962
Bsh1285I CGRYCG 3 cut(s) 379, 1755, 2648
BshFI GGCC 9 cut(s) 1159, 1262, 1592, 1599, 2000, 2479, 2857, 3305, 3462
BshNI GGYRCC 5 cut(s) 120, 536, 894, 1404, 2973
BshTI ACCGGT 1 cut(s) 1527
BsiEI CGRYCG 3 cut(s) 379, 1755, 2648
BsiHKAI GWGCWC 6 cut(s) 547, 1572, 1819, 1866, 3563, 4393
BsiHKCI CYCGRG 2 cut(s) 1593, 4261
BsiSI CCGG 4 cut(s) 1528, 1960, 2697, 3671
BslFI GGGAC 6 cut(s) 81, 922, 1261, 1563, 3756, 4479
BsmAI GTCTC 7 cut(s) 204, 1031, 1292, 1562, 1902, 2157, 2688
BsmBI CGTCTC 3 cut(s) 204, 1292, 2688
BsmFI GGGAC 6 cut(s) 81, 922, 1261, 1563, 3756, 4479
BsnI GGCC 9 cut(s) 1159, 1262, 1592, 1599, 2000, 2479, 2857, 3305, 3462
Bso31I GGTCTC 2 cut(s) 1902, 2157
BsoBI CYCGRG 2 cut(s) 1593, 4261
Bsp1286I GDGCHC 8 cut(s) 547, 1409, 1572, 1819, 1866, 3563, 4393, 4537
Bsp13I TCCGGA 1 cut(s) 1959
Bsp1407I TGTACA 1 cut(s) 1246
Bsp1720I GCTNAGC 1 cut(s) 1860
Bsp19I CCATGG 2 cut(s) 1600, 3804
BspANI GGCC 9 cut(s) 1159, 1262, 1592, 1599, 2000, 2479, 2857, 3305, 3462
BspCNI CTCAG 6 cut(s) 264, 961, 1288, 1852, 2023, 3712
BspEI TCCGGA 1 cut(s) 1959
BspHI TCATGA 2 cut(s) 990, 3420
BspLI GGNNCC 9 cut(s) 122, 500, 538, 608, 896, 1406, 2975, 3089, 4467
BspMI ACCTGC 4 cut(s) 1297, 2031, 2112, 3693
BspPI GGATC 5 cut(s) 479, 601, 614, 1653, 4403
BspQI GCTCTTC 1 cut(s) 552
BspT107I GGYRCC 5 cut(s) 120, 536, 894, 1404, 2973
BspTNI GGTCTC 2 cut(s) 1902, 2157
BsrDI GCAATG 2 cut(s) 1875, 2133
BsrFI RCCGGY 2 cut(s) 1527, 2696
BsrGI TGTACA 1 cut(s) 1246
BssAI RCCGGY 2 cut(s) 1527, 2696
BssT1I CCWWGG 7 cut(s) 26, 898, 1354, 1409, 1600, 2740, 3804
Bst2UI CCWGG 4 cut(s) 2859, 3189, 3504, 4395
Bst6I CTCTTC 5 cut(s) 202, 552, 937, 2432, 3419
BstAPI GCANNNNNTGC 1 cut(s) 2059
BstAUI TGTACA 1 cut(s) 1246
BstBAI YACGTR 1 cut(s) 2007
BstC8I GCNNGC 6 cut(s) 699, 726, 936, 1235, 1367, 4219
BstDSI CCRYGG 2 cut(s) 1600, 3804
BstEII GGTNACC 1 cut(s) 1444
BstH2I RGCGCY 1 cut(s) 1540
BstHHI GCGC 3 cut(s) 1539, 1804, 4027
BstMAI GTCTC 7 cut(s) 204, 1031, 1292, 1562, 1902, 2157, 2688
BstMCI CGRYCG 3 cut(s) 379, 1755, 2648
BstNI CCWGG 4 cut(s) 2859, 3189, 3504, 4395
BstNSI RCATGY 3 cut(s) 1373, 1846, 2782
BstPI GGTNACC 1 cut(s) 1444
BstSCI CCNGG 5 cut(s) 2857, 3187, 3502, 3670, 4393
BstSFI CTRYAG 3 cut(s) 2088, 3339, 3564
BstSLI GKGCMC 3 cut(s) 1409, 1819, 4537
BstSNI TACGTA 1 cut(s) 2007
BstV2I GAAGAC 4 cut(s) 222, 548, 2542, 2906
BstX2I RGATCY 2 cut(s) 606, 1645
BstXI CCANNNNNNTGG 3 cut(s) 905, 2220, 4332
BstYI RGATCY 2 cut(s) 606, 1645
Bsu36I CCTNAGG 2 cut(s) 969, 3083
BsuI GTATCC 2 cut(s) 779, 1981
BsuRI GGCC 9 cut(s) 1159, 1262, 1592, 1599, 2000, 2479, 2857, 3305, 3462
BtgI CCRYGG 2 cut(s) 1600, 3804
BtgZI GCGATG 3 cut(s) 321, 562, 4383
BtrI CACGTC 1 cut(s) 169
BtsI GCAGTG 3 cut(s) 2352, 3755, 4482
BveI ACCTGC 4 cut(s) 1297, 2031, 2112, 3693
Cac8I GCNNGC 6 cut(s) 699, 726, 936, 1235, 1367, 4219
CaiI CAGNNNCTG 1 cut(s) 1514
CciI TCATGA 2 cut(s) 990, 3420
CfoI GCGC 3 cut(s) 1539, 1804, 4027
Cfr10I RCCGGY 2 cut(s) 1527, 2696
Cfr13I GGNCC 8 cut(s) 1157, 1358, 1591, 2124, 3303, 3452, 3460, 4466
CseI GACGC 2 cut(s) 690, 1777
Csp6I GTAC 8 cut(s) 1247, 1373, 2008, 2458, 2974, 3660, 4000, 4426
CspAI ACCGGT 1 cut(s) 1527
CspCI CAANNNNNGTGG 2 cut(s) 1910, 1945
CviQI GTAC 8 cut(s) 1247, 1373, 2008, 2458, 2974, 3660, 4000, 4426
DraI TTTAAA 1 cut(s) 7
DrdI GACNNNNNNGTC 1 cut(s) 201
DseDI GACNNNNNNGTC 1 cut(s) 201
EaeI YGGCCR 1 cut(s) 1998
Eam1104I CTCTTC 5 cut(s) 202, 552, 937, 2432, 3419
EarI CTCTTC 5 cut(s) 202, 552, 937, 2432, 3419
EciI GGCGGA 1 cut(s) 684
Ecl136II GAGCTC 4 cut(s) 545, 1570, 1864, 3561
Eco105I TACGTA 1 cut(s) 2007
Eco130I CCWWGG 7 cut(s) 26, 898, 1354, 1409, 1600, 2740, 3804
Eco147I AGGCCT 1 cut(s) 2857
Eco24I GRGCYC 4 cut(s) 547, 1572, 1866, 3563
Eco31I GGTCTC 2 cut(s) 1902, 2157
Eco32I GATATC 5 cut(s) 1110, 1188, 1321, 3031, 3391
Eco47I GGWCC 4 cut(s) 1358, 2124, 3452, 4466
Eco47III AGCGCT 1 cut(s) 1538
Eco53kI GAGCTC 4 cut(s) 545, 1570, 1864, 3561
Eco57I CTGAAG 3 cut(s) 2301, 2517, 2807
Eco81I CCTNAGG 2 cut(s) 969, 3083
Eco88I CYCGRG 2 cut(s) 1593, 4261
Eco91I GGTNACC 1 cut(s) 1444
EcoICRI GAGCTC 4 cut(s) 545, 1570, 1864, 3561
EcoO65I GGTNACC 1 cut(s) 1444
EcoRI GAATTC 5 cut(s) 1693, 2551, 2650, 3575, 3898
EcoRII CCWGG 4 cut(s) 2857, 3187, 3502, 4393
EcoRV GATATC 5 cut(s) 1110, 1188, 1321, 3031, 3391
EcoT14I CCWWGG 7 cut(s) 26, 898, 1354, 1409, 1600, 2740, 3804
EcoT38I GRGCYC 4 cut(s) 547, 1572, 1866, 3563
ErhI CCWWGG 7 cut(s) 26, 898, 1354, 1409, 1600, 2740, 3804
Esp3I CGTCTC 3 cut(s) 204, 1292, 2688
FaqI GGGAC 6 cut(s) 81, 922, 1261, 1563, 3756, 4479
FauI CCCGC 3 cut(s) 2141, 2917, 4210
FauNDI CATATG 1 cut(s) 2254
FbaI TGATCA 2 cut(s) 1833, 2130
FblI GTMKAC 2 cut(s) 2775, 3342
FriOI GRGCYC 4 cut(s) 547, 1572, 1866, 3563
GlaI GCGC 3 cut(s) 1538, 1803, 4026
GsaI CCCAGC 2 cut(s) 738, 3996
GsuI CTGGAG 3 cut(s) 1397, 1544, 2133
HaeII RGCGCY 1 cut(s) 1540
HaeIII GGCC 9 cut(s) 1159, 1262, 1592, 1599, 2000, 2479, 2857, 3305, 3462
HapII CCGG 4 cut(s) 1528, 1960, 2697, 3671
HgaI GACGC 2 cut(s) 690, 1777
HhaI GCGC 3 cut(s) 1539, 1804, 4027
Hin6I GCGC 3 cut(s) 1537, 1802, 4025
HinP1I GCGC 3 cut(s) 1537, 1802, 4025
HincII GTYRAC 3 cut(s) 2764, 3207, 3228
HindII GTYRAC 3 cut(s) 2764, 3207, 3228
HindIII AAGCTT 1 cut(s) 3639
HpaI GTTAAC 2 cut(s) 2764, 3228
HpaII CCGG 4 cut(s) 1528, 1960, 2697, 3671
HphI GGTGA 4 cut(s) 1154, 1246, 2235, 3568
Hpy99I CGWCG 2 cut(s) 198, 2202
HpyCH4IV ACGT 5 cut(s) 168, 354, 1001, 1301, 2006
HpySE526I ACGT 5 cut(s) 168, 354, 1001, 1301, 2006
HspAI GCGC 3 cut(s) 1537, 1802, 4025
Kpn2I TCCGGA 1 cut(s) 1959
KpnI GGTACC 1 cut(s) 2977
Ksp22I TGATCA 2 cut(s) 1833, 2130
KspAI GTTAAC 2 cut(s) 2764, 3228
LguI GCTCTTC 1 cut(s) 552
LweI GCATC 8 cut(s) 291, 637, 1086, 3160, 3286, 3537, 3634, 4238
MaeII ACGT 5 cut(s) 168, 354, 1001, 1301, 2006
MaeIII GTNAC 8 cut(s) 1132, 1444, 1510, 1747, 2839, 2942, 3046, 4356
MfeI CAATTG 1 cut(s) 3231
MflI RGATCY 2 cut(s) 606, 1645
MhlI GDGCHC 8 cut(s) 547, 1409, 1572, 1819, 1866, 3563, 4393, 4537
MlsI TGGCCA 1 cut(s) 2000
MluNI TGGCCA 1 cut(s) 2000
MlyI GAGTC 2 cut(s) 673, 2892
MmeI TCCRAC 3 cut(s) 506, 2101, 4436
Mox20I TGGCCA 1 cut(s) 2000
MroI TCCGGA 1 cut(s) 1959
MroXI GAANNNNTTC 1 cut(s) 1529
MscI TGGCCA 1 cut(s) 2000
MslI CAYNNNNRTG 3 cut(s) 1841, 3269, 3317
Msp20I TGGCCA 1 cut(s) 2000
MspA1I CMGCKG 2 cut(s) 2827, 3308
MspI CCGG 4 cut(s) 1528, 1960, 2697, 3671
MspR9I CCNGG 5 cut(s) 2859, 3189, 3504, 3672, 4395
MunI CAATTG 1 cut(s) 3231
MvaI CCWGG 4 cut(s) 2859, 3189, 3504, 4395
NciI CCSGG 1 cut(s) 3672
NcoI CCATGG 2 cut(s) 1600, 3804
NdeI CATATG 1 cut(s) 2254
NlaIV GGNNCC 9 cut(s) 122, 500, 538, 608, 896, 1406, 2975, 3089, 4467
NmeAIII GCCGAG 1 cut(s) 61
NmuCI GTSAC 4 cut(s) 1510, 1747, 3046, 4356
NspI RCATGY 3 cut(s) 1373, 1846, 2782
PagI TCATGA 2 cut(s) 990, 3420
PceI AGGCCT 1 cut(s) 2857
PciI ACATGT 1 cut(s) 1842
PciSI GCTCTTC 1 cut(s) 552
PcsI WCGNNNNNNNCGW 2 cut(s) 202, 372
PdmI GAANNNNTTC 1 cut(s) 1529
PfeI GAWTC 8 cut(s) 584, 1963, 1982, 2464, 2678, 3059, 4347, 4480
PflFI GACNNNGTC 1 cut(s) 1554
PflMI CCANNNNNTGG 4 cut(s) 508, 2083, 4299, 4422
PfoI TCCNGGA 2 cut(s) 3187, 3502
PinAI ACCGGT 1 cut(s) 1527
Ple19I CGATCG 1 cut(s) 2648
PleI GAGTC 2 cut(s) 673, 2891
PpsI GAGTC 2 cut(s) 673, 2891
Ppu21I YACGTR 1 cut(s) 2007
PscI ACATGT 1 cut(s) 1842
Psp124BI GAGCTC 4 cut(s) 547, 1572, 1866, 3563
Psp6I CCWGG 4 cut(s) 2857, 3187, 3502, 4393
PspEI GGTNACC 1 cut(s) 1444
PspFI CCCAGC 2 cut(s) 734, 3992
PspGI CCWGG 4 cut(s) 2857, 3187, 3502, 4393
PspN4I GGNNCC 9 cut(s) 122, 500, 538, 608, 896, 1406, 2975, 3089, 4467
PspPI GGNCC 8 cut(s) 1157, 1358, 1591, 2124, 3303, 3452, 3460, 4466
PstNI CAGNNNCTG 1 cut(s) 1514
PsuI RGATCY 2 cut(s) 606, 1645
PsyI GACNNNGTC 1 cut(s) 1554
PvuI CGATCG 1 cut(s) 2648
PvuII CAGCTG 1 cut(s) 2827
RsaI GTAC 8 cut(s) 1248, 1374, 2009, 2459, 2975, 3661, 4001, 4427
RsaNI GTAC 8 cut(s) 1247, 1373, 2008, 2458, 2974, 3660, 4000, 4426
RseI CAYNNNNRTG 3 cut(s) 1841, 3269, 3317
SacI GAGCTC 4 cut(s) 547, 1572, 1866, 3563
SapI GCTCTTC 1 cut(s) 552
Sau96I GGNCC 8 cut(s) 1157, 1358, 1591, 2124, 3303, 3452, 3460, 4466
SchI GAGTC 2 cut(s) 673, 2892
ScrFI CCNGG 5 cut(s) 2859, 3189, 3504, 3672, 4395
SduI GDGCHC 8 cut(s) 547, 1409, 1572, 1819, 1866, 3563, 4393, 4537
SfaNI GCATC 8 cut(s) 291, 637, 1086, 3160, 3286, 3537, 3634, 4238
SfcI CTRYAG 3 cut(s) 2088, 3339, 3564
SinI GGWCC 4 cut(s) 1358, 2124, 3452, 4466
SmiMI CAYNNNNRTG 3 cut(s) 1841, 3269, 3317
SmlI CTYRAG 5 cut(s) 641, 1450, 1762, 2048, 3164
SmoI CTYRAG 5 cut(s) 641, 1450, 1762, 2048, 3164
SnaBI TACGTA 1 cut(s) 2007
SpeI ACTAGT 1 cut(s) 2446
SseBI AGGCCT 1 cut(s) 2857
SspI AATATT 3 cut(s) 963, 1472, 2358
SstI GAGCTC 4 cut(s) 547, 1572, 1866, 3563
StuI AGGCCT 1 cut(s) 2857
StyD4I CCNGG 5 cut(s) 2857, 3187, 3502, 3670, 4393
StyI CCWWGG 7 cut(s) 26, 898, 1354, 1409, 1600, 2740, 3804
TaiI ACGT 5 cut(s) 171, 357, 1004, 1304, 2009
TatI WGTACW 4 cut(s) 1246, 1372, 3659, 3999
TauI GCSGC 2 cut(s) 115, 3308
TfiI GAWTC 8 cut(s) 584, 1963, 1982, 2464, 2678, 3059, 4347, 4480
TseFI GTSAC 4 cut(s) 1510, 1747, 3046, 4356
Tsp45I GTSAC 4 cut(s) 1510, 1747, 3046, 4356
TspGWI ACGGA 1 cut(s) 4191
Tth111I GACNNNGTC 1 cut(s) 1554
Van91I CCANNNNNTGG 4 cut(s) 508, 2083, 4299, 4422
VneI GTGCAC 1 cut(s) 1815
VpaK11BI GGWCC 4 cut(s) 1358, 2124, 3452, 4466
XbaI TCTAGA 2 cut(s) 877, 1781
XceI RCATGY 3 cut(s) 1373, 1846, 2782
XcmI CCANNNNNNNNNTGG 3 cut(s) 68, 2379, 2716
XmaJI CCTAGG 1 cut(s) 1409
XmiI GTMKAC 2 cut(s) 2775, 3342
XmnI GAANNNNTTC 1 cut(s) 1529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.