AT1G60670

Protein of unknown function (DUF3755)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004772
Physical Location & Seq
Forward (+)
22343585 .. 22347624
4040 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G60670.3

Sequence Viewer

Length: 606 bp
ATGATTCCGATGGATAATTATTGCGTACCGAGTACTAGCACGACCGGTTTAGTGTTTTCGGCGACTTCGAGCATGAATGCTTCTTCTGGATTTCATCTTACTGTAAATTCTCCGACTTCGGTTACTGGACTCAAACATGAAGCTTCTTTGGCTGTTGATTGGTCTGTTGAGGAACAATATATATTGGAGAAAGGTCTTTCAAAATTTAAAGATGAACCACAAGTTACAAAGTATGTAAAGATCGCAGCAACTCTACCAGATAAAAGTGTACGGGATGTAGCAATGAGATGTAAATGGATGACGCAAAAGCGAAGAAAAGGGGAAGAACATAGTACTGGCACGAAGGTTAGTTATAGAAAGGTAGTGGATTTACCCCCAAAACTGAACATGTTCTCAACTGAGCCACAACAAAATGCTACATATGCCATGAACCATATGTGCCAGAGTGCACGCATGCCTTTTGAAGGTCTAAGTGATGCGGTTATGGAGCGTCTACGGCAGAATGCTCAAGCTTTCAGTCAAATCTCCTCAAACCTTTCTGTGTGCAAGCATGAAGGAGATGCCTGGGATCATCAGCCGGATGCCGCCTCTGCCTGTCTCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3755

Protein Analysis

201

Amino Acids

22.31

Weight (kDa)

7.66

Isoelectric Point (pI)

36.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3755 PF12579 177 - 209 3.1e-16 Protein of unknown function (DUF3755)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 493
AciI CCGC 2 cut(s) 479, 585
AclWI GGATC 1 cut(s) 576
AcsI RAATTY 2 cut(s) 106, 203
AfaI GTAC 4 cut(s) 27, 34, 270, 334
AfiI CCNNNNNNNGG 1 cut(s) 464
AflIII ACRYGT 1 cut(s) 387
AgeI ACCGGT 1 cut(s) 44
AgsI TTSAA 2 cut(s) 201, 464
AjnI CCWGG 1 cut(s) 563
AluBI AGCT 2 cut(s) 143, 512
AluI AGCT 2 cut(s) 143, 512
Alw21I GWGCWC 1 cut(s) 451
Alw26I GTCTC 1 cut(s) 602
Alw44I GTGCAC 1 cut(s) 447
AlwI GGATC 1 cut(s) 576
ApaLI GTGCAC 1 cut(s) 447
ApeKI GCWGC 1 cut(s) 245
ApoI RAATTY 2 cut(s) 106, 203
AsiGI ACCGGT 1 cut(s) 44
Asp700I GAANNNNTTC 1 cut(s) 389
BaeGI GKGCMC 1 cut(s) 451
Bbv12I GWGCWC 1 cut(s) 451
BbvI GCAGC 1 cut(s) 257
BccI CCATC 1 cut(s) 4
BceAI ACGGC 1 cut(s) 512
BciT130I CCWGG 1 cut(s) 565
BcoDI GTCTC 1 cut(s) 602
BfaI CTAG 1 cut(s) 36
BisI GCNGC 2 cut(s) 246, 585
BlsI GCNGC 2 cut(s) 247, 586
BmcAI AGTACT 2 cut(s) 34, 334
Bme1390I CCNGG 1 cut(s) 565
BmrFI CCNGG 1 cut(s) 565
BmsI GCATC 3 cut(s) 466, 550, 571
BpuEI CTTGAG 1 cut(s) 492
BsaJI CCNNGG 1 cut(s) 564
BsaWI WCCGGW 1 cut(s) 44
Bsc4I CCNNNNNNNGG 1 cut(s) 464
Bse118I RCCGGY 1 cut(s) 44
Bse1I ACTGG 2 cut(s) 130, 340
Bse3DI GCAATG 1 cut(s) 288
BseBI CCWGG 1 cut(s) 565
BseDI CCNNGG 1 cut(s) 564
BseGI GGATG 3 cut(s) 280, 303, 586
BseLI CCNNNNNNNGG 1 cut(s) 464
BseMI GCAATG 1 cut(s) 288
BseMII CTCAG 1 cut(s) 390
BseNI ACTGG 2 cut(s) 130, 340
BseRI GAGGAG 1 cut(s) 517
BseSI GKGCMC 1 cut(s) 451
BseXI GCAGC 1 cut(s) 257
Bsh1285I CGRYCG 1 cut(s) 45
BshTI ACCGGT 1 cut(s) 44
BsiEI CGRYCG 1 cut(s) 45
BsiHKAI GWGCWC 1 cut(s) 451
BsiSI CCGG 2 cut(s) 45, 578
BslI CCNNNNNNNGG 1 cut(s) 464
BsmAI GTCTC 1 cut(s) 602
BsmI GAATGC 2 cut(s) 82, 508
Bsp1286I GDGCHC 1 cut(s) 451
Bsp143I GATC 2 cut(s) 240, 568
BspACI CCGC 2 cut(s) 479, 585
BspCNI CTCAG 1 cut(s) 391
BspPI GGATC 1 cut(s) 576
BsrDI GCAATG 1 cut(s) 288
BsrFI RCCGGY 1 cut(s) 44
BsrI ACTGG 2 cut(s) 130, 340
BssAI RCCGGY 1 cut(s) 44
BssECI CCNNGG 1 cut(s) 564
BssMI GATC 2 cut(s) 240, 568
Bst2UI CCWGG 1 cut(s) 565
Bst4CI ACNGT 1 cut(s) 103
BstC8I GCNNGC 3 cut(s) 451, 455, 548
BstDEI CTNAG 2 cut(s) 399, 470
BstENI CCTNNNNNAGG 1 cut(s) 462
BstF5I GGATG 3 cut(s) 280, 303, 586
BstKTI GATC 2 cut(s) 243, 571
BstMAI GTCTC 1 cut(s) 602
BstMBI GATC 2 cut(s) 240, 568
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 4 cut(s) 149, 422, 496, 590
BstNI CCWGG 1 cut(s) 565
BstNSI RCATGY 2 cut(s) 391, 457
BstSCI CCNGG 1 cut(s) 563
BstSLI GKGCMC 1 cut(s) 451
BstV1I GCAGC 1 cut(s) 257
BtsCI GGATG 3 cut(s) 280, 303, 586
Cac8I GCNNGC 3 cut(s) 451, 455, 548
Cfr10I RCCGGY 1 cut(s) 44
CseI GACGC 2 cut(s) 310, 479
Csp6I GTAC 4 cut(s) 26, 33, 269, 333
CspAI ACCGGT 1 cut(s) 44
CviAII CATG 6 cut(s) 73, 137, 388, 427, 454, 551
CviJI RGCY 5 cut(s) 143, 152, 403, 512, 577
CviKI_1 RGCY 5 cut(s) 143, 152, 403, 512, 577
CviQI GTAC 4 cut(s) 26, 33, 269, 333
DdeI CTNAG 2 cut(s) 399, 470
DpnI GATC 2 cut(s) 242, 570
DpnII GATC 2 cut(s) 240, 568
DraI TTTAAA 1 cut(s) 208
EcoNI CCTNNNNNAGG 1 cut(s) 462
EcoRII CCWGG 1 cut(s) 563
FaeI CATG 6 cut(s) 76, 140, 391, 430, 457, 554
FatI CATG 6 cut(s) 72, 136, 387, 426, 453, 550
FauNDI CATATG 2 cut(s) 421, 435
FblI GTMKAC 1 cut(s) 493
Fnu4HI GCNGC 2 cut(s) 246, 585
FokI GGATG 3 cut(s) 287, 310, 593
Fsp4HI GCNGC 2 cut(s) 246, 585
FspBI CTAG 1 cut(s) 36
GluI GCNGC 2 cut(s) 246, 585
HapII CCGG 2 cut(s) 45, 578
HgaI GACGC 2 cut(s) 310, 479
Hin1II CATG 6 cut(s) 76, 140, 391, 430, 457, 554
HindIII AAGCTT 2 cut(s) 141, 510
HinfI GANTC 2 cut(s) 4, 129
HpaII CCGG 2 cut(s) 45, 578
Hpy166II GTNNAC 3 cut(s) 269, 449, 494
Hpy188I TCNGA 2 cut(s) 9, 114
Hpy188III TCNNGA 1 cut(s) 87
Hpy8I GTNNAC 3 cut(s) 269, 449, 494
HpyAV CCTTC 3 cut(s) 337, 458, 548
HpyCH4III ACNGT 1 cut(s) 103
HpyCH4V TGCA 2 cut(s) 449, 546
HpyF10VI GCNNNNNNNGC 4 cut(s) 149, 422, 496, 590
HpyF3I CTNAG 2 cut(s) 399, 470
Hsp92II CATG 6 cut(s) 76, 140, 391, 430, 457, 554
Kzo9I GATC 2 cut(s) 240, 568
LmnI GCTCC 1 cut(s) 487
LpnPI CCDG 9 cut(s) 58, 72, 111, 270, 321, 455, 550, 577, 591
Lsp1109I GCAGC 1 cut(s) 257
LweI GCATC 3 cut(s) 466, 550, 571
MaeI CTAG 1 cut(s) 36
MaeIII GTNAC 2 cut(s) 121, 223
MalI GATC 2 cut(s) 242, 570
MboI GATC 2 cut(s) 240, 568
MboII GAAGA 3 cut(s) 75, 324, 335
MhlI GDGCHC 1 cut(s) 451
MluCI AATT 4 cut(s) 16, 106, 203, 601
MlyI GAGTC 1 cut(s) 123
MmeI TCCRAC 1 cut(s) 137
MnlI CCTC 3 cut(s) 163, 538, 598
MroXI GAANNNNTTC 1 cut(s) 389
MseI TTAA 2 cut(s) 207, 604
MspI CCGG 2 cut(s) 45, 578
MspR9I CCNGG 1 cut(s) 565
Mva1269I GAATGC 2 cut(s) 82, 508
MvaI CCWGG 1 cut(s) 565
MwoI GCNNNNNNNGC 4 cut(s) 149, 422, 496, 590
NdeI CATATG 2 cut(s) 421, 435
NdeII GATC 2 cut(s) 240, 568
NlaIII CATG 6 cut(s) 76, 140, 391, 430, 457, 554
NspI RCATGY 2 cut(s) 391, 457
PaeI GCATGC 1 cut(s) 457
PciI ACATGT 1 cut(s) 387
PcsI WCGNNNNNNNCGW 1 cut(s) 65
PctI GAATGC 2 cut(s) 82, 508
PdmI GAANNNNTTC 1 cut(s) 389
PfeI GAWTC 1 cut(s) 4
PinAI ACCGGT 1 cut(s) 44
PkrI GCNGC 2 cut(s) 247, 586
PleI GAGTC 1 cut(s) 123
PpsI GAGTC 1 cut(s) 123
PscI ACATGT 1 cut(s) 387
Psp6I CCWGG 1 cut(s) 563
PspGI CCWGG 1 cut(s) 563
RsaI GTAC 4 cut(s) 27, 34, 270, 334
RsaNI GTAC 4 cut(s) 26, 33, 269, 333
SaqAI TTAA 2 cut(s) 207, 604
SatI GCNGC 2 cut(s) 246, 585
Sau3AI GATC 2 cut(s) 240, 568
ScaI AGTACT 2 cut(s) 34, 334
SchI GAGTC 1 cut(s) 123
ScrFI CCNGG 1 cut(s) 565
SduI GDGCHC 1 cut(s) 451
SetI ASST 7 cut(s) 145, 196, 348, 363, 469, 514, 537
SfaNI GCATC 3 cut(s) 466, 550, 571
SmlI CTYRAG 1 cut(s) 507
SmoI CTYRAG 1 cut(s) 507
SphI GCATGC 1 cut(s) 457
Sse9I AATT 4 cut(s) 16, 106, 203, 601
SsiI CCGC 2 cut(s) 479, 585
SspMI CTAG 1 cut(s) 36
StyD4I CCNGG 1 cut(s) 563
TaaI ACNGT 1 cut(s) 103
TaqI TCGA 1 cut(s) 68
TasI AATT 4 cut(s) 16, 106, 203, 601
TatI WGTACW 2 cut(s) 32, 332
TauI GCSGC 1 cut(s) 587
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 2 cut(s) 207, 604
Tru9I TTAA 2 cut(s) 207, 604
TseI GCWGC 1 cut(s) 245
TspDTI ATGAA 6 cut(s) 83, 89, 153, 228, 443, 567
VneI GTGCAC 1 cut(s) 447
XagI CCTNNNNNAGG 1 cut(s) 462
XapI RAATTY 2 cut(s) 106, 203
XceI RCATGY 2 cut(s) 391, 457
XmiI GTMKAC 1 cut(s) 493
XmnI GAANNNNTTC 1 cut(s) 389
XspI CTAG 1 cut(s) 36
ZrmI AGTACT 2 cut(s) 34, 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.