MD13G1008900.v1.1

Protein of unknown function (DUF3755)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004772
Physical Location & Seq
Reverse (-)
543342 .. 547872
4531 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1008900.v1.1.491

Sequence Viewer

Length: 837 bp
ATGGCGATGGAGTCGAACACAGGATATCTCCGCGACGAAGCTTTTGGCTACGGTTTGAACCGACACGCTATATCGTTTCAGTCCGGGGCCATACACAGCAGCTCGGAGATGATTCCGATGGGGAATTATTTTGCTACGGACGGGGGCATGATGTTTTCGTCGAATTCGGGCATAGTTAACAACAATCCTGTAATTAGTCAGGCTGGGAATTCATCAGGTTCTCTGCTTCTCGATTCGGTCCCCGGGCTCAAGCATGACACAGGCTTGGCTGTTGAGTGGTCTGTGGAGGAACAGTACAAATTGACGGAGGGCCTTGTCAAATATGCTGATGAACCAAGTATTATGAAATACATAAAGATCGCGGCAATGTTACATGATAAAACTGTGCGTGATGTTGCTTTGAGGTGTAGGTGGATGACTAGAAAGCGTAGGAAACCTGAAGAACATAGTATGGGAAAGAAGGGGAACATTAGGAAGGATAAACTGGTGGAGTCGTCTACAACGACAAACATACATACAGCTGCACCACTGGACATGGCTGCATGTTCTCATTCGGTGTACCATATGGACCAGAGTGAGCGTTTGCAGTATGAAGGATTAAGTGGCACATTCAAGCATCTATTGGAGCAAAATGCTCAAGCTTTCAGTCAAATTACATCTAATCTTTCATCATACAAGTTGCAGGAAAACATTGACCTCTTCTACCGCACGAGGAACAATTTAACTGCCATCCTTAACGACATGAGAGGTATGCCTGGCATAATGAGCCGAATGCCTCCCCTGCCACCCATTGACGAGGATCTTGCTAATAGTCTCTTGCACAAATCAAGGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3755

Protein Analysis

279

Amino Acids

31.01

Weight (kDa)

7.16

Isoelectric Point (pI)

52.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3755 PF12579 224 - 257 3.7e-18 Protein of unknown function (DUF3755)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 497
AccII CGCG 2 cut(s) 33, 362
AciI CCGC 3 cut(s) 31, 362, 706
AclWI GGATC 1 cut(s) 807
AcsI RAATTY 2 cut(s) 163, 208
AcuI CTGAAG 1 cut(s) 459
AfaI GTAC 2 cut(s) 296, 560
AgsI TTSAA 2 cut(s) 58, 613
AjnI CCWGG 1 cut(s) 754
AluBI AGCT 4 cut(s) 41, 102, 521, 641
AluI AGCT 4 cut(s) 41, 102, 521, 641
Alw26I GTCTC 1 cut(s) 818
AlwI GGATC 1 cut(s) 807
Ama87I CYCGRG 1 cut(s) 242
AoxI GGCC 2 cut(s) 87, 310
ApeKI GCWGC 3 cut(s) 99, 521, 539
ApoI RAATTY 2 cut(s) 163, 208
ArsI GACNNNNNNTTYG 2 cut(s) 26, 58
AspS9I GGNCC 5 cut(s) 87, 238, 310, 568, 831
AsuC2I CCSGG 3 cut(s) 85, 243, 244
AvaI CYCGRG 1 cut(s) 242
AvaII GGWCC 3 cut(s) 238, 568, 831
BanII GRGCYC 1 cut(s) 249
BauI CACGAG 1 cut(s) 709
BbvI GCAGC 3 cut(s) 111, 508, 526
BccI CCATC 2 cut(s) 112, 737
BcgI CGANNNNNNTGC 2 cut(s) 785, 819
BciT130I CCWGG 1 cut(s) 756
BcnI CCSGG 3 cut(s) 85, 243, 244
BcoDI GTCTC 1 cut(s) 818
BfaI CTAG 1 cut(s) 420
BisI GCNGC 4 cut(s) 100, 363, 522, 540
BlsI GCNGC 4 cut(s) 101, 364, 523, 541
Bme1390I CCNGG 4 cut(s) 85, 243, 244, 756
Bme18I GGWCC 3 cut(s) 238, 568, 831
BmeT110I CYCGRG 1 cut(s) 242
BmgT120I GGNCC 5 cut(s) 87, 238, 310, 568, 831
BmiI GGNNCC 2 cut(s) 88, 240
BmrFI CCNGG 4 cut(s) 85, 243, 244, 756
BmsI GCATC 1 cut(s) 625
BpuEI CTTGAG 2 cut(s) 233, 621
BpuMI CCSGG 3 cut(s) 85, 243, 244
BsaJI CCNNGG 3 cut(s) 84, 241, 242
BsaXI ACNNNNNCTCC 2 cut(s) 278, 308
Bse1I ACTGG 2 cut(s) 489, 534
Bse3DI GCAATG 1 cut(s) 372
BseBI CCWGG 1 cut(s) 756
BseDI CCNNGG 3 cut(s) 84, 241, 242
BseGI GGATG 2 cut(s) 420, 729
BseMI GCAATG 1 cut(s) 372
BseNI ACTGG 2 cut(s) 489, 534
BseXI GCAGC 3 cut(s) 111, 508, 526
BseYI CCCAGC 1 cut(s) 203
BsgI GTGCAG 1 cut(s) 507
Bsh1236I CGCG 2 cut(s) 33, 362
BshFI GGCC 2 cut(s) 89, 312
BsiHKCI CYCGRG 1 cut(s) 242
BsiSI CCGG 2 cut(s) 84, 243
BslFI GGGAC 1 cut(s) 224
BsmAI GTCTC 1 cut(s) 818
BsmFI GGGAC 1 cut(s) 224
BsmI GAATGC 1 cut(s) 777
BsnI GGCC 2 cut(s) 89, 312
BsoBI CYCGRG 1 cut(s) 242
Bsp1286I GDGCHC 1 cut(s) 249
Bsp143I GATC 2 cut(s) 357, 799
BspACI CCGC 3 cut(s) 31, 362, 706
BspANI GGCC 2 cut(s) 89, 312
BspFNI CGCG 2 cut(s) 33, 362
BspLI GGNNCC 2 cut(s) 88, 240
BspPI GGATC 1 cut(s) 807
BsrDI GCAATG 1 cut(s) 372
BsrI ACTGG 2 cut(s) 489, 534
BssECI CCNNGG 3 cut(s) 84, 241, 242
BssMI GATC 2 cut(s) 357, 799
BssSI CACGAG 1 cut(s) 709
Bst2BI CACGAG 1 cut(s) 709
Bst2UI CCWGG 1 cut(s) 756
Bst4CI ACNGT 3 cut(s) 53, 294, 385
Bst6I CTCTTC 1 cut(s) 704
BstF5I GGATG 2 cut(s) 420, 729
BstFNI CGCG 2 cut(s) 33, 362
BstKTI GATC 2 cut(s) 360, 802
BstMAI GTCTC 1 cut(s) 818
BstMBI GATC 2 cut(s) 357, 799
BstMWI GCNNNNNNNGC 2 cut(s) 765, 781
BstNI CCWGG 1 cut(s) 756
BstNSI RCATGY 1 cut(s) 546
BstSCI CCNGG 4 cut(s) 83, 241, 242, 754
BstUI CGCG 2 cut(s) 33, 362
BstV1I GCAGC 3 cut(s) 111, 508, 526
BstX2I RGATCY 1 cut(s) 799
BstYI RGATCY 1 cut(s) 799
BsuRI GGCC 2 cut(s) 89, 312
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 2 cut(s) 420, 729
BtsIMutI CAGTG 1 cut(s) 527
Cfr13I GGNCC 5 cut(s) 87, 238, 310, 568, 831
Cfr9I CCCGGG 1 cut(s) 242
Csp6I GTAC 2 cut(s) 295, 559
CviAII CATG 6 cut(s) 148, 254, 374, 535, 543, 742
CviQI GTAC 2 cut(s) 295, 559
DpnI GATC 2 cut(s) 359, 801
DpnII GATC 2 cut(s) 357, 799
Eam1104I CTCTTC 1 cut(s) 704
EarI CTCTTC 1 cut(s) 704
Eco24I GRGCYC 1 cut(s) 249
Eco32I GATATC 1 cut(s) 26
Eco47I GGWCC 3 cut(s) 238, 568, 831
Eco57I CTGAAG 1 cut(s) 459
Eco88I CYCGRG 1 cut(s) 242
EcoO109I RGGNCCY 2 cut(s) 310, 831
EcoRI GAATTC 2 cut(s) 163, 208
EcoRII CCWGG 1 cut(s) 754
EcoRV GATATC 1 cut(s) 26
EcoT38I GRGCYC 1 cut(s) 249
FaeI CATG 6 cut(s) 151, 257, 377, 538, 546, 745
FaqI GGGAC 1 cut(s) 224
FatI CATG 6 cut(s) 147, 253, 373, 534, 542, 741
FauNDI CATATG 1 cut(s) 564
FblI GTMKAC 1 cut(s) 497
Fnu4HI GCNGC 4 cut(s) 100, 363, 522, 540
FokI GGATG 2 cut(s) 427, 716
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 4 cut(s) 100, 363, 522, 540
FspBI CTAG 1 cut(s) 420
GluI GCNGC 4 cut(s) 100, 363, 522, 540
GsaI CCCAGC 1 cut(s) 207
HaeIII GGCC 2 cut(s) 89, 312
HapII CCGG 2 cut(s) 84, 243
Hin1II CATG 6 cut(s) 151, 257, 377, 538, 546, 745
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HindIII AAGCTT 2 cut(s) 39, 639
HinfI GANTC 4 cut(s) 11, 112, 233, 491
HpaI GTTAAC 1 cut(s) 178
HpaII CCGG 2 cut(s) 84, 243
Hpy166II GTNNAC 3 cut(s) 178, 498, 559
Hpy188I TCNGA 2 cut(s) 106, 117
Hpy188III TCNNGA 2 cut(s) 230, 834
Hpy8I GTNNAC 3 cut(s) 178, 498, 559
Hpy99I CGWCG 2 cut(s) 38, 163
HpyAV CCTTC 3 cut(s) 454, 469, 587
HpyCH4III ACNGT 3 cut(s) 53, 294, 385
HpyCH4V TGCA 5 cut(s) 524, 542, 586, 682, 820
HpyF10VI GCNNNNNNNGC 2 cut(s) 765, 781
Hsp92II CATG 6 cut(s) 151, 257, 377, 538, 546, 745
KspAI GTTAAC 1 cut(s) 178
Kzo9I GATC 2 cut(s) 357, 799
LmnI GCTCC 1 cut(s) 625
Lsp1109I GCAGC 3 cut(s) 111, 508, 526
LweI GCATC 1 cut(s) 625
MaeI CTAG 1 cut(s) 420
MaeIII GTNAC 1 cut(s) 369
MalI GATC 2 cut(s) 359, 801
MboI GATC 2 cut(s) 357, 799
MboII GAAGA 2 cut(s) 452, 691
MflI RGATCY 1 cut(s) 799
MhlI GDGCHC 1 cut(s) 249
MluCI AATT 7 cut(s) 124, 163, 192, 208, 299, 651, 718
MlyI GAGTC 2 cut(s) 20, 500
MnlI CCTC 8 cut(s) 280, 301, 396, 705, 707, 740, 786, 790
MseI TTAA 4 cut(s) 177, 599, 722, 735
MspA1I CMGCKG 1 cut(s) 521
MspI CCGG 2 cut(s) 84, 243
MspR9I CCNGG 4 cut(s) 85, 243, 244, 756
Mva1269I GAATGC 1 cut(s) 777
MvaI CCWGG 1 cut(s) 756
MvnI CGCG 2 cut(s) 33, 362
MwoI GCNNNNNNNGC 2 cut(s) 765, 781
NciI CCSGG 3 cut(s) 85, 243, 244
NdeI CATATG 1 cut(s) 564
NdeII GATC 2 cut(s) 357, 799
NlaIII CATG 6 cut(s) 151, 257, 377, 538, 546, 745
NlaIV GGNNCC 2 cut(s) 88, 240
NspI RCATGY 1 cut(s) 546
PcsI WCGNNNNNNNCGW 1 cut(s) 500
PctI GAATGC 1 cut(s) 777
PfeI GAWTC 2 cut(s) 112, 233
PkrI GCNGC 4 cut(s) 101, 364, 523, 541
PleI GAGTC 2 cut(s) 19, 499
PpsI GAGTC 2 cut(s) 19, 499
PpuMI RGGWCCY 1 cut(s) 831
Psp5II RGGWCCY 1 cut(s) 831
Psp6I CCWGG 1 cut(s) 754
PspFI CCCAGC 1 cut(s) 203
PspGI CCWGG 1 cut(s) 754
PspN4I GGNNCC 2 cut(s) 88, 240
PspPI GGNCC 5 cut(s) 87, 238, 310, 568, 831
PspPPI RGGWCCY 1 cut(s) 831
PsuI RGATCY 1 cut(s) 799
PvuII CAGCTG 1 cut(s) 521
RsaI GTAC 2 cut(s) 296, 560
RsaNI GTAC 2 cut(s) 295, 559
SaqAI TTAA 4 cut(s) 177, 599, 722, 735
SatI GCNGC 4 cut(s) 100, 363, 522, 540
Sau3AI GATC 2 cut(s) 357, 799
Sau96I GGNCC 5 cut(s) 87, 238, 310, 568, 831
SchI GAGTC 2 cut(s) 20, 500
ScrFI CCNGG 4 cut(s) 85, 243, 244, 756
SduI GDGCHC 1 cut(s) 249
SfaNI GCATC 1 cut(s) 625
SinI GGWCC 3 cut(s) 238, 568, 831
SmaI CCCGGG 1 cut(s) 244
SmlI CTYRAG 2 cut(s) 248, 636
SmoI CTYRAG 2 cut(s) 248, 636
Sse9I AATT 7 cut(s) 124, 163, 192, 208, 299, 651, 718
SsiI CCGC 3 cut(s) 31, 362, 706
SspMI CTAG 1 cut(s) 420
StyD4I CCNGG 4 cut(s) 83, 241, 242, 754
TaaI ACNGT 3 cut(s) 53, 294, 385
TaqI TCGA 3 cut(s) 14, 161, 231
TaqII GACCGA 1 cut(s) 226
TasI AATT 7 cut(s) 124, 163, 192, 208, 299, 651, 718
TatI WGTACW 1 cut(s) 294
TauI GCSGC 1 cut(s) 365
TfiI GAWTC 2 cut(s) 112, 233
Tru1I TTAA 4 cut(s) 177, 599, 722, 735
Tru9I TTAA 4 cut(s) 177, 599, 722, 735
TscAI CASTG 1 cut(s) 534
TseI GCWGC 3 cut(s) 99, 521, 539
TspDTI ATGAA 5 cut(s) 201, 345, 359, 606, 657
TspGWI ACGGA 2 cut(s) 152, 320
TspMI CCCGGG 1 cut(s) 242
TspRI CASTG 1 cut(s) 534
VpaK11BI GGWCC 3 cut(s) 238, 568, 831
XapI RAATTY 2 cut(s) 163, 208
XceI RCATGY 1 cut(s) 546
XmaI CCCGGG 1 cut(s) 242
XmiI GTMKAC 1 cut(s) 497
XspI CTAG 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.