Rroxscaffold_5G00386640

Protein of unknown function (DUF3755)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004772
Physical Location & Seq
Forward (+)
65476697 .. 65481957
5261 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00386640.1

Sequence Viewer

Length: 906 bp
ATGGCGATGGAGTCGAACACCGGGTTTCTACATGATGAGACTTTTGGCTATGGCTTGAACCGGCACGCGATATCTTTCCAGTCTGGAGCGATAAACAGCAGCTCGGAGATGATTCCGATGGGGAATTACTTTAGTACTGACCCGGTGATGGGGATGGGGATGGGGATGATGTTCTCTCCGAATTCGGGGTCTGGCATGGGCATGGGCATGGTCAACCACAGTCCTGTAATTAGTCAACCCGGGACTTCATCAGGGGCTTCTCTGCTTGTTGATTCGGTACCGGGCCTCAAGCATGACACAGGCTTGGCAGTTGAGTGGTCTATGGAGGAGCAATACAAATTGGAGGAGGGCCTTGTCAAATTTGCTGATGAACCAAGTACTATGAGATATGCTGATGAACCGAGTATTATGAGGTACATAAAGATTGCGGCAACCTTGCGTGATAAAACTGTGCGTGATGTTGCCTTGAGGTGTAGGTGGATGACAAGAAAGCGGAGGAAACCTGAAGACCATACTACAGGAAAGAAGGGCAACATTAGGAAGGATAAACTGGTAGATTCATACTCCAAGATGAGCATACCCTCAGCTCCACAACACAACATGGCTGCATATTCTCTTATGATGCATCGTATGAACCAGAATGAGCGTTTGCAGTGTGAAGGAATAAGTGGGACAGCCAAGCATTTATTGGACCAAAATGCTCAAGCTTTTAATCAAATCACAGCTAACCTTTCTACGTACAAGTTACAGGACAATATTAACCTCTTCTGTCGCACAAGGAGCAACTTAACTGCCATCTTGAATGACATGAGAGGTATGCCTGGCTTAATGAGCCAAATGCCACCGCTGGATGTATGCATAAATGAGGACCTTGCTAATAGTGTTATCTTGCCTAATACAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3755

Protein Analysis

301

Amino Acids

33.36

Weight (kDa)

7.09

Isoelectric Point (pI)

47.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3755 PF12579 246 - 279 2e-18 Protein of unknown function (DUF3755)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 277
AccB1I GGYRCC 1 cut(s) 277
AccII CGCG 1 cut(s) 68
AciI CCGC 3 cut(s) 428, 493, 845
AcsI RAATTY 2 cut(s) 181, 359
AcuI CTGAAG 1 cut(s) 525
AfaI GTAC 5 cut(s) 136, 279, 379, 416, 740
AfiI CCNNNNNNNGG 3 cut(s) 148, 149, 185
AgsI TTSAA 2 cut(s) 58, 802
AjnI CCWGG 1 cut(s) 820
AluBI AGCT 4 cut(s) 102, 587, 707, 725
AluI AGCT 4 cut(s) 102, 587, 707, 725
Alw26I GTCTC 1 cut(s) 32
Ama87I CYCGRG 1 cut(s) 239
AoxI GGCC 2 cut(s) 283, 349
ApeKI GCWGC 2 cut(s) 99, 605
ApoI RAATTY 2 cut(s) 181, 359
ArsI GACNNNNNNTTYG 2 cut(s) 173, 205
Asp718I GGTACC 1 cut(s) 277
AspS9I GGNCC 4 cut(s) 283, 349, 691, 868
AsuC2I CCSGG 5 cut(s) 22, 143, 240, 241, 282
AsuHPI GGTGA 1 cut(s) 157
AvaI CYCGRG 1 cut(s) 239
AvaII GGWCC 2 cut(s) 691, 868
BanI GGYRCC 1 cut(s) 277
BbsI GAAGAC 1 cut(s) 513
BbvCI CCTCAGC 1 cut(s) 583
BbvI GCAGC 2 cut(s) 111, 592
BccI CCATC 5 cut(s) 112, 142, 148, 154, 803
BciT130I CCWGG 1 cut(s) 822
BcnI CCSGG 5 cut(s) 22, 143, 240, 241, 282
BcoDI GTCTC 1 cut(s) 32
BfmI CTRYAG 1 cut(s) 516
BisI GCNGC 3 cut(s) 100, 429, 606
BlsI GCNGC 3 cut(s) 101, 430, 607
BmcAI AGTACT 2 cut(s) 136, 379
Bme1390I CCNGG 6 cut(s) 22, 143, 240, 241, 282, 822
Bme18I GGWCC 2 cut(s) 691, 868
BmeT110I CYCGRG 1 cut(s) 239
BmgT120I GGNCC 4 cut(s) 283, 349, 691, 868
BmiI GGNNCC 1 cut(s) 279
BmrFI CCNGG 6 cut(s) 22, 143, 240, 241, 282, 822
BmsI GCATC 2 cut(s) 612, 634
BpiI GAAGAC 1 cut(s) 513
BpmI CTGGAG 1 cut(s) 105
Bpu10I CCTNAGC 1 cut(s) 583
BpuEI CTTGAG 3 cut(s) 272, 487, 687
BpuMI CCSGG 5 cut(s) 22, 143, 240, 241, 282
BsaAI YACGTR 1 cut(s) 738
BsaJI CCNNGG 1 cut(s) 239
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 149, 185
Bse118I RCCGGY 1 cut(s) 60
Bse1I ACTGG 2 cut(s) 79, 555
BseBI CCWGG 1 cut(s) 822
BseDI CCNNGG 1 cut(s) 239
BseGI GGATG 5 cut(s) 159, 165, 171, 486, 856
BseLI CCNNNNNNNGG 3 cut(s) 148, 149, 185
BseMII CTCAG 1 cut(s) 597
BseNI ACTGG 2 cut(s) 79, 555
BseRI GAGGAG 2 cut(s) 341, 359
BseXI GCAGC 2 cut(s) 111, 592
Bsh1236I CGCG 1 cut(s) 68
BshFI GGCC 2 cut(s) 285, 351
BshNI GGYRCC 1 cut(s) 277
BsiHKCI CYCGRG 1 cut(s) 239
BsiSI CCGG 5 cut(s) 21, 61, 143, 240, 281
BslFI GGGAC 2 cut(s) 256, 685
BslI CCNNNNNNNGG 3 cut(s) 148, 149, 185
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 2 cut(s) 256, 685
BsnI GGCC 2 cut(s) 285, 351
BsoBI CYCGRG 1 cut(s) 239
BspACI CCGC 3 cut(s) 428, 493, 845
BspANI GGCC 2 cut(s) 285, 351
BspCNI CTCAG 1 cut(s) 596
BspFNI CGCG 1 cut(s) 68
BspLI GGNNCC 1 cut(s) 279
BspT107I GGYRCC 1 cut(s) 277
BsrFI RCCGGY 1 cut(s) 60
BsrI ACTGG 2 cut(s) 79, 555
BssAI RCCGGY 1 cut(s) 60
BssECI CCNNGG 1 cut(s) 239
Bst2UI CCWGG 1 cut(s) 822
Bst4CI ACNGT 2 cut(s) 221, 451
Bst6I CTCTTC 1 cut(s) 770
BstBAI YACGTR 1 cut(s) 738
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 2 cut(s) 583, 903
BstF5I GGATG 5 cut(s) 159, 165, 171, 486, 856
BstFNI CGCG 1 cut(s) 68
BstMAI GTCTC 1 cut(s) 32
BstMWI GCNNNNNNNGC 2 cut(s) 780, 831
BstNI CCWGG 1 cut(s) 822
BstSCI CCNGG 6 cut(s) 20, 141, 238, 239, 280, 820
BstSFI CTRYAG 1 cut(s) 516
BstSNI TACGTA 1 cut(s) 738
BstUI CGCG 1 cut(s) 68
BstV1I GCAGC 2 cut(s) 111, 592
BstV2I GAAGAC 1 cut(s) 513
BsuRI GGCC 2 cut(s) 285, 351
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 5 cut(s) 159, 165, 171, 486, 856
BtsI GCAGTG 1 cut(s) 659
BtsIMutI CAGTG 1 cut(s) 659
Cac8I GCNNGC 1 cut(s) 66
Cfr10I RCCGGY 1 cut(s) 60
Cfr13I GGNCC 4 cut(s) 283, 349, 691, 868
Cfr9I CCCGGG 1 cut(s) 239
Csp6I GTAC 5 cut(s) 135, 278, 378, 415, 739
CviAII CATG 7 cut(s) 32, 196, 202, 208, 293, 601, 808
CviQI GTAC 5 cut(s) 135, 278, 378, 415, 739
DdeI CTNAG 2 cut(s) 583, 903
Eam1104I CTCTTC 1 cut(s) 770
EarI CTCTTC 1 cut(s) 770
Eco105I TACGTA 1 cut(s) 738
Eco32I GATATC 1 cut(s) 72
Eco47I GGWCC 2 cut(s) 691, 868
Eco57I CTGAAG 1 cut(s) 525
Eco88I CYCGRG 1 cut(s) 239
EcoO109I RGGNCCY 2 cut(s) 349, 868
EcoRI GAATTC 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 820
EcoRV GATATC 1 cut(s) 72
EcoT22I ATGCAT 2 cut(s) 627, 860
FaeI CATG 7 cut(s) 35, 199, 205, 211, 296, 604, 811
FaqI GGGAC 2 cut(s) 256, 685
FatI CATG 7 cut(s) 31, 195, 201, 207, 292, 600, 807
Fnu4HI GCNGC 3 cut(s) 100, 429, 606
FokI GGATG 5 cut(s) 166, 172, 178, 493, 863
Fsp4HI GCNGC 3 cut(s) 100, 429, 606
GluI GCNGC 3 cut(s) 100, 429, 606
GsuI CTGGAG 1 cut(s) 105
HaeIII GGCC 2 cut(s) 285, 351
HapII CCGG 5 cut(s) 21, 61, 143, 240, 281
Hin1II CATG 7 cut(s) 35, 199, 205, 211, 296, 604, 811
HincII GTYRAC 2 cut(s) 214, 236
HindII GTYRAC 2 cut(s) 214, 236
HindIII AAGCTT 1 cut(s) 705
HinfI GANTC 4 cut(s) 11, 112, 272, 557
HpaII CCGG 5 cut(s) 21, 61, 143, 240, 281
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 2 cut(s) 214, 236
Hpy188I TCNGA 3 cut(s) 106, 117, 180
Hpy188III TCNNGA 2 cut(s) 84, 799
Hpy8I GTNNAC 2 cut(s) 214, 236
HpyAV CCTTC 3 cut(s) 520, 535, 653
HpyCH4III ACNGT 2 cut(s) 221, 451
HpyCH4IV ACGT 1 cut(s) 737
HpyCH4V TGCA 4 cut(s) 608, 625, 652, 858
HpyF10VI GCNNNNNNNGC 2 cut(s) 780, 831
HpyF3I CTNAG 2 cut(s) 583, 903
HpySE526I ACGT 1 cut(s) 737
Hsp92II CATG 7 cut(s) 35, 199, 205, 211, 296, 604, 811
KpnI GGTACC 1 cut(s) 281
LmnI GCTCC 4 cut(s) 86, 328, 592, 780
Lsp1109I GCAGC 2 cut(s) 111, 592
LweI GCATC 2 cut(s) 612, 634
MaeII ACGT 1 cut(s) 737
MaeIII GTNAC 1 cut(s) 744
MboII GAAGA 2 cut(s) 518, 757
MluCI AATT 5 cut(s) 124, 181, 228, 338, 359
MlyI GAGTC 1 cut(s) 20
Mph1103I ATGCAT 2 cut(s) 627, 860
MseI TTAA 4 cut(s) 711, 759, 788, 827
MslI CAYNNNNRTG 2 cut(s) 200, 206
MspA1I CMGCKG 1 cut(s) 847
MspI CCGG 5 cut(s) 21, 61, 143, 240, 281
MspR9I CCNGG 6 cut(s) 22, 143, 240, 241, 282, 822
MvaI CCWGG 1 cut(s) 822
MvnI CGCG 1 cut(s) 68
MwoI GCNNNNNNNGC 2 cut(s) 780, 831
NciI CCSGG 5 cut(s) 22, 143, 240, 241, 282
NlaIII CATG 7 cut(s) 35, 199, 205, 211, 296, 604, 811
NlaIV GGNNCC 1 cut(s) 279
NsiI ATGCAT 2 cut(s) 627, 860
PfeI GAWTC 3 cut(s) 112, 272, 557
PkrI GCNGC 3 cut(s) 101, 430, 607
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
Ppu21I YACGTR 1 cut(s) 738
PpuMI RGGWCCY 1 cut(s) 868
Psp5II RGGWCCY 1 cut(s) 868
Psp6I CCWGG 1 cut(s) 820
PspGI CCWGG 1 cut(s) 820
PspN4I GGNNCC 1 cut(s) 279
PspPI GGNCC 4 cut(s) 283, 349, 691, 868
PspPPI RGGWCCY 1 cut(s) 868
RsaI GTAC 5 cut(s) 136, 279, 379, 416, 740
RsaNI GTAC 5 cut(s) 135, 278, 378, 415, 739
RseI CAYNNNNRTG 2 cut(s) 200, 206
SaqAI TTAA 4 cut(s) 711, 759, 788, 827
SatI GCNGC 3 cut(s) 100, 429, 606
Sau96I GGNCC 4 cut(s) 283, 349, 691, 868
ScaI AGTACT 2 cut(s) 136, 379
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 6 cut(s) 22, 143, 240, 241, 282, 822
SfaNI GCATC 2 cut(s) 612, 634
SfcI CTRYAG 1 cut(s) 516
SinI GGWCC 2 cut(s) 691, 868
SmaI CCCGGG 1 cut(s) 241
SmiMI CAYNNNNRTG 2 cut(s) 200, 206
SmlI CTYRAG 3 cut(s) 287, 466, 702
SmoI CTYRAG 3 cut(s) 287, 466, 702
SnaBI TACGTA 1 cut(s) 738
Sse9I AATT 5 cut(s) 124, 181, 228, 338, 359
SsiI CCGC 3 cut(s) 428, 493, 845
SspI AATATT 1 cut(s) 757
StyD4I CCNGG 6 cut(s) 20, 141, 238, 239, 280, 820
TaaI ACNGT 2 cut(s) 221, 451
TaiI ACGT 1 cut(s) 740
TaqI TCGA 1 cut(s) 14
TasI AATT 5 cut(s) 124, 181, 228, 338, 359
TatI WGTACW 2 cut(s) 134, 377
TauI GCSGC 1 cut(s) 431
TfiI GAWTC 3 cut(s) 112, 272, 557
Tru1I TTAA 4 cut(s) 711, 759, 788, 827
Tru9I TTAA 4 cut(s) 711, 759, 788, 827
TscAI CASTG 1 cut(s) 659
TseI GCWGC 2 cut(s) 99, 605
TspDTI ATGAA 5 cut(s) 237, 384, 411, 549, 647
TspMI CCCGGG 1 cut(s) 239
TspRI CASTG 1 cut(s) 659
VpaK11BI GGWCC 2 cut(s) 691, 868
XapI RAATTY 2 cut(s) 181, 359
XcmI CCANNNNNNNNNTGG 1 cut(s) 685
XmaI CCCGGG 1 cut(s) 239
ZrmI AGTACT 2 cut(s) 136, 379
Zsp2I ATGCAT 2 cut(s) 627, 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.