Rh4BG440300

Protein of unknown function (DUF3755)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0004772
Physical Location & Seq
Forward (+)
58060527 .. 58065732
5206 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG440300.1

Sequence Viewer

Length: 873 bp
ATGGCGATGGAGTCGAACACCGGGTTTCTACATGATGAGACTTTTGGCTATGGCTTGAACCGGCACGCGATATCTTTCCAGTCTGGAGCAATAAACAGCAGCTCGGAGATGATTCCGATGGGGAATTACTTTAGTACTGACCCGGTGATGGGGATGGGGATGATGTTCTCTCCGAATTTGGGGTCAGGCATGGGCATGGGCATGGTCAACCACAGTCCTGTAATTAGTCAACCCGGGACTTCATCAGGGGCTTCTCTGCTTGTTGATTCGGTACCGGGCCTCAAGCATGACACAGGCTTGGCAGTTGAGTGGTCTATGGAGGAGCAATACAAATTGGAGGAGGGCCTTGTCAAGTATGCTGATGAACCGAGTATTATGAGGTACATAAAGATTGCGGCAACCTTGCGTGATAAAACTGTGCGTGATGTTGCCTTGAGGTGTAGGTGGATGACGAGAAAGCGAAGGAAACCTGAAGACCATACTACAGGAAAGAAGGGCAACATTAGGAAGGATAAACTGGTAGAATCATACTCCAAGATGAGCATACCCTCAGCTCCACAACACAACATGGCTGCATATTCTCTTATGATGCATCGTATGAACCAGAATGAGCGTTTGCAGTGTGAAGGAATAAGTGGGACAGCCAAGCATTTATTGGACCAAAATGCTCAAGCTTTTAATCAAATCACAGCTAACCTTTCTACGTACAAGTTACAGGACAATATTAACCTCTTCTGTCGCACAAGGAACAACTTAACTGCCATCTTGAATGACATGAGAGGTATGCCTGGCTTAATGAGCCAAATGCCACCGCTGGATGTATGCATAAATGAGGACCTTGCTAATAGTGTTATCTTGCGTAATACAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3755

Protein Analysis

290

Amino Acids

32.27

Weight (kDa)

8.3

Isoelectric Point (pI)

48.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3755 PF12579 235 - 268 7.2e-19 Protein of unknown function (DUF3755)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 271
AccB1I GGYRCC 1 cut(s) 271
AccII CGCG 1 cut(s) 68
AciI CCGC 2 cut(s) 395, 812
AcsI RAATTY 1 cut(s) 175
AcuI CTGAAG 1 cut(s) 492
AfaI GTAC 4 cut(s) 136, 273, 383, 707
AfiI CCNNNNNNNGG 3 cut(s) 148, 149, 179
AgsI TTSAA 2 cut(s) 58, 769
AjnI CCWGG 1 cut(s) 787
AluBI AGCT 4 cut(s) 102, 554, 674, 692
AluI AGCT 4 cut(s) 102, 554, 674, 692
Alw26I GTCTC 1 cut(s) 32
Ama87I CYCGRG 1 cut(s) 233
AoxI GGCC 2 cut(s) 277, 343
ApeKI GCWGC 2 cut(s) 99, 572
ApoI RAATTY 1 cut(s) 175
ArsI GACNNNNNNTTYG 2 cut(s) 167, 199
Asp718I GGTACC 1 cut(s) 271
AspS9I GGNCC 4 cut(s) 277, 343, 658, 835
AsuC2I CCSGG 5 cut(s) 22, 143, 234, 235, 276
AsuHPI GGTGA 1 cut(s) 157
AvaI CYCGRG 1 cut(s) 233
AvaII GGWCC 2 cut(s) 658, 835
BanI GGYRCC 1 cut(s) 271
BbsI GAAGAC 1 cut(s) 480
BbvCI CCTCAGC 1 cut(s) 550
BbvI GCAGC 2 cut(s) 111, 559
BccI CCATC 4 cut(s) 112, 142, 148, 770
BciT130I CCWGG 1 cut(s) 789
BcnI CCSGG 5 cut(s) 22, 143, 234, 235, 276
BcoDI GTCTC 1 cut(s) 32
BfmI CTRYAG 1 cut(s) 483
BisI GCNGC 3 cut(s) 100, 396, 573
BlsI GCNGC 3 cut(s) 101, 397, 574
BmcAI AGTACT 1 cut(s) 136
Bme1390I CCNGG 6 cut(s) 22, 143, 234, 235, 276, 789
Bme18I GGWCC 2 cut(s) 658, 835
BmeT110I CYCGRG 1 cut(s) 233
BmgT120I GGNCC 4 cut(s) 277, 343, 658, 835
BmiI GGNNCC 1 cut(s) 273
BmrFI CCNGG 6 cut(s) 22, 143, 234, 235, 276, 789
BmsI GCATC 2 cut(s) 579, 601
BpiI GAAGAC 1 cut(s) 480
BpmI CTGGAG 1 cut(s) 105
Bpu10I CCTNAGC 1 cut(s) 550
BpuEI CTTGAG 3 cut(s) 266, 454, 654
BpuMI CCSGG 5 cut(s) 22, 143, 234, 235, 276
BsaAI YACGTR 1 cut(s) 705
BsaJI CCNNGG 1 cut(s) 233
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 149, 179
Bse118I RCCGGY 1 cut(s) 60
Bse1I ACTGG 2 cut(s) 79, 522
BseBI CCWGG 1 cut(s) 789
BseDI CCNNGG 1 cut(s) 233
BseGI GGATG 4 cut(s) 159, 165, 453, 823
BseLI CCNNNNNNNGG 3 cut(s) 148, 149, 179
BseMII CTCAG 1 cut(s) 564
BseNI ACTGG 2 cut(s) 79, 522
BseRI GAGGAG 2 cut(s) 335, 353
BseXI GCAGC 2 cut(s) 111, 559
Bsh1236I CGCG 1 cut(s) 68
BshFI GGCC 2 cut(s) 279, 345
BshNI GGYRCC 1 cut(s) 271
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 5 cut(s) 21, 61, 143, 234, 275
BslFI GGGAC 2 cut(s) 250, 652
BslI CCNNNNNNNGG 3 cut(s) 148, 149, 179
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 2 cut(s) 250, 652
BsnI GGCC 2 cut(s) 279, 345
BsoBI CYCGRG 1 cut(s) 233
BspACI CCGC 2 cut(s) 395, 812
BspANI GGCC 2 cut(s) 279, 345
BspCNI CTCAG 1 cut(s) 563
BspFNI CGCG 1 cut(s) 68
BspLI GGNNCC 1 cut(s) 273
BspT107I GGYRCC 1 cut(s) 271
BsrFI RCCGGY 1 cut(s) 60
BsrI ACTGG 2 cut(s) 79, 522
BssAI RCCGGY 1 cut(s) 60
BssECI CCNNGG 1 cut(s) 233
Bst2UI CCWGG 1 cut(s) 789
Bst4CI ACNGT 2 cut(s) 215, 418
Bst6I CTCTTC 1 cut(s) 737
BstBAI YACGTR 1 cut(s) 705
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 2 cut(s) 550, 870
BstF5I GGATG 4 cut(s) 159, 165, 453, 823
BstFNI CGCG 1 cut(s) 68
BstMAI GTCTC 1 cut(s) 32
BstMWI GCNNNNNNNGC 1 cut(s) 798
BstNI CCWGG 1 cut(s) 789
BstSCI CCNGG 6 cut(s) 20, 141, 232, 233, 274, 787
BstSFI CTRYAG 1 cut(s) 483
BstSNI TACGTA 1 cut(s) 705
BstUI CGCG 1 cut(s) 68
BstV1I GCAGC 2 cut(s) 111, 559
BstV2I GAAGAC 1 cut(s) 480
BsuRI GGCC 2 cut(s) 279, 345
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 4 cut(s) 159, 165, 453, 823
BtsI GCAGTG 1 cut(s) 626
BtsIMutI CAGTG 1 cut(s) 626
Cac8I GCNNGC 1 cut(s) 66
Cfr10I RCCGGY 1 cut(s) 60
Cfr13I GGNCC 4 cut(s) 277, 343, 658, 835
Cfr9I CCCGGG 1 cut(s) 233
Csp6I GTAC 4 cut(s) 135, 272, 382, 706
CviAII CATG 7 cut(s) 32, 190, 196, 202, 287, 568, 775
CviQI GTAC 4 cut(s) 135, 272, 382, 706
DdeI CTNAG 2 cut(s) 550, 870
Eam1104I CTCTTC 1 cut(s) 737
EarI CTCTTC 1 cut(s) 737
Eco105I TACGTA 1 cut(s) 705
Eco32I GATATC 1 cut(s) 72
Eco47I GGWCC 2 cut(s) 658, 835
Eco57I CTGAAG 1 cut(s) 492
Eco88I CYCGRG 1 cut(s) 233
EcoO109I RGGNCCY 2 cut(s) 343, 835
EcoRII CCWGG 1 cut(s) 787
EcoRV GATATC 1 cut(s) 72
EcoT22I ATGCAT 2 cut(s) 594, 827
FaeI CATG 7 cut(s) 35, 193, 199, 205, 290, 571, 778
FaqI GGGAC 2 cut(s) 250, 652
FatI CATG 7 cut(s) 31, 189, 195, 201, 286, 567, 774
Fnu4HI GCNGC 3 cut(s) 100, 396, 573
FokI GGATG 4 cut(s) 166, 172, 460, 830
Fsp4HI GCNGC 3 cut(s) 100, 396, 573
GluI GCNGC 3 cut(s) 100, 396, 573
GsuI CTGGAG 1 cut(s) 105
HaeIII GGCC 2 cut(s) 279, 345
HapII CCGG 5 cut(s) 21, 61, 143, 234, 275
Hin1II CATG 7 cut(s) 35, 193, 199, 205, 290, 571, 778
HincII GTYRAC 2 cut(s) 208, 230
HindII GTYRAC 2 cut(s) 208, 230
HindIII AAGCTT 1 cut(s) 672
HinfI GANTC 4 cut(s) 11, 112, 266, 524
HpaII CCGG 5 cut(s) 21, 61, 143, 234, 275
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 2 cut(s) 208, 230
Hpy188I TCNGA 3 cut(s) 106, 117, 174
Hpy188III TCNNGA 2 cut(s) 84, 766
Hpy8I GTNNAC 2 cut(s) 208, 230
HpyAV CCTTC 4 cut(s) 456, 487, 502, 620
HpyCH4III ACNGT 2 cut(s) 215, 418
HpyCH4IV ACGT 1 cut(s) 704
HpyCH4V TGCA 4 cut(s) 575, 592, 619, 825
HpyF10VI GCNNNNNNNGC 1 cut(s) 798
HpyF3I CTNAG 2 cut(s) 550, 870
HpySE526I ACGT 1 cut(s) 704
Hsp92II CATG 7 cut(s) 35, 193, 199, 205, 290, 571, 778
KpnI GGTACC 1 cut(s) 275
LmnI GCTCC 3 cut(s) 86, 322, 559
Lsp1109I GCAGC 2 cut(s) 111, 559
LweI GCATC 2 cut(s) 579, 601
MaeII ACGT 1 cut(s) 704
MaeIII GTNAC 1 cut(s) 711
MboII GAAGA 2 cut(s) 485, 724
MluCI AATT 4 cut(s) 124, 175, 222, 332
MlyI GAGTC 1 cut(s) 20
Mph1103I ATGCAT 2 cut(s) 594, 827
MseI TTAA 4 cut(s) 678, 726, 755, 794
MslI CAYNNNNRTG 2 cut(s) 194, 200
MspA1I CMGCKG 1 cut(s) 814
MspI CCGG 5 cut(s) 21, 61, 143, 234, 275
MspR9I CCNGG 6 cut(s) 22, 143, 234, 235, 276, 789
MvaI CCWGG 1 cut(s) 789
MvnI CGCG 1 cut(s) 68
MwoI GCNNNNNNNGC 1 cut(s) 798
NciI CCSGG 5 cut(s) 22, 143, 234, 235, 276
NlaIII CATG 7 cut(s) 35, 193, 199, 205, 290, 571, 778
NlaIV GGNNCC 1 cut(s) 273
NsiI ATGCAT 2 cut(s) 594, 827
PfeI GAWTC 3 cut(s) 112, 266, 524
PkrI GCNGC 3 cut(s) 101, 397, 574
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
Ppu21I YACGTR 1 cut(s) 705
PpuMI RGGWCCY 1 cut(s) 835
Psp5II RGGWCCY 1 cut(s) 835
Psp6I CCWGG 1 cut(s) 787
PspGI CCWGG 1 cut(s) 787
PspN4I GGNNCC 1 cut(s) 273
PspPI GGNCC 4 cut(s) 277, 343, 658, 835
PspPPI RGGWCCY 1 cut(s) 835
RsaI GTAC 4 cut(s) 136, 273, 383, 707
RsaNI GTAC 4 cut(s) 135, 272, 382, 706
RseI CAYNNNNRTG 2 cut(s) 194, 200
SaqAI TTAA 4 cut(s) 678, 726, 755, 794
SatI GCNGC 3 cut(s) 100, 396, 573
Sau96I GGNCC 4 cut(s) 277, 343, 658, 835
ScaI AGTACT 1 cut(s) 136
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 6 cut(s) 22, 143, 234, 235, 276, 789
SfaNI GCATC 2 cut(s) 579, 601
SfcI CTRYAG 1 cut(s) 483
SinI GGWCC 2 cut(s) 658, 835
SmaI CCCGGG 1 cut(s) 235
SmiMI CAYNNNNRTG 2 cut(s) 194, 200
SmlI CTYRAG 3 cut(s) 281, 433, 669
SmoI CTYRAG 3 cut(s) 281, 433, 669
SnaBI TACGTA 1 cut(s) 705
Sse9I AATT 4 cut(s) 124, 175, 222, 332
SsiI CCGC 2 cut(s) 395, 812
SspI AATATT 1 cut(s) 724
StyD4I CCNGG 6 cut(s) 20, 141, 232, 233, 274, 787
TaaI ACNGT 2 cut(s) 215, 418
TaiI ACGT 1 cut(s) 707
TaqI TCGA 1 cut(s) 14
TasI AATT 4 cut(s) 124, 175, 222, 332
TatI WGTACW 1 cut(s) 134
TauI GCSGC 1 cut(s) 398
TfiI GAWTC 3 cut(s) 112, 266, 524
Tru1I TTAA 4 cut(s) 678, 726, 755, 794
Tru9I TTAA 4 cut(s) 678, 726, 755, 794
TscAI CASTG 1 cut(s) 626
TseI GCWGC 2 cut(s) 99, 572
TspDTI ATGAA 3 cut(s) 231, 378, 614
TspMI CCCGGG 1 cut(s) 233
TspRI CASTG 1 cut(s) 626
VpaK11BI GGWCC 2 cut(s) 658, 835
XapI RAATTY 1 cut(s) 175
XcmI CCANNNNNNNNNTGG 1 cut(s) 652
XmaI CCCGGG 1 cut(s) 233
ZrmI AGTACT 1 cut(s) 136
Zsp2I ATGCAT 2 cut(s) 594, 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.