FvH4_4g36480

Protein of unknown function (DUF3755)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004772
Physical Location & Seq
Forward (+)
33407049 .. 33411342
4294 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g36480.t1

Sequence Viewer

Length: 912 bp
ATGGCGATGCAGTCCAACACAGGGTTTCTGCATGATGAGACTTTTGGGTATGGCTTGAACCGACACGCCATATCTTTCCAGTCTGGAGCTATAAACAGCAGCTCGGAGATGTTTCCGATGGGGAGTTACTACAGTGCTGACCATGGGATGGGGATGGGAGTGGGGATGGGGATGGGGATGATGTTTTCTCCGAATTCGGGGCCAGCCATGAGCATGGTCAACCACCGTCCTGTAATTAGTCAACCTGTGAACTCATCAGGAGCTTCTCTCCTTCTTGATTCCGCGCCGGGGCTCAAGCATGACACAGGCTTGGCAGTCGAGTGGTCTATGGAGGAGCAGTACAAATTGGAGGAGGGCCTTGTCAAATTTGCTGATGAACCAAGTACTATGAGATATGCTGATGAACCAAGTATTATGAGGTACATAAAGATTGCTGCAACCTTGCGTGATAAAACTGTGCGTGATGTTGCCTTGAGGTGTAGGTGGATGACGAGAAAGCGGAGGAAAGCTGAAGACCATACTACGGGAAAGAAGGGCAACATTCGGAAGGATAAACTGGTAGAATCATCCTCCAAGATAAGCATACCCTCAGCTCCACAATACAATATGGCTGCGTATTCTCGTATGATGCATCGAATGGACCAGAATGAGCGCTTGCAGTGTGAAGGAATAAGTGGGACGGCCAAGCAGTTATTGGACCAAAATGCTCAGGCTTTTAATCAAATCACAGCCAACCTTTCTACGTACAAGTTACAGGACAACATTGACCTATTTTCCTGTACAAGGAACAACTTAAATGACATCTTGAATGACATGAGAGGTATGCCTGGCCTAATGAGCCAAATGCCCCCGCTGGACGTATGCATAAATGAGGATCTTGCTAATCGTGTTACCTTGCGTAGTTCAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3755

Protein Analysis

304

Amino Acids

33.72

Weight (kDa)

7.7

Isoelectric Point (pI)

52.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3755 PF12579 248 - 281 7.5e-16 Protein of unknown function (DUF3755)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 148
AccII CGCG 1 cut(s) 284
AciI CCGC 3 cut(s) 282, 499, 851
AclWI GGATC 1 cut(s) 882
AcoI YGGCCR 1 cut(s) 681
AcsI RAATTY 2 cut(s) 193, 365
AcuI CTGAAG 1 cut(s) 531
AfaI GTAC 5 cut(s) 341, 385, 422, 746, 781
AfeI AGCGCT 1 cut(s) 653
AfiI CCNNNNNNNGG 6 cut(s) 21, 148, 197, 288, 523, 783
AgsI TTSAA 3 cut(s) 58, 808, 906
AjnI CCWGG 1 cut(s) 826
AluBI AGCT 5 cut(s) 89, 102, 263, 509, 593
AluI AGCT 5 cut(s) 89, 102, 263, 509, 593
Alw26I GTCTC 1 cut(s) 32
AlwI GGATC 1 cut(s) 882
Aor51HI AGCGCT 1 cut(s) 653
AoxI GGCC 4 cut(s) 200, 355, 681, 829
ApeKI GCWGC 3 cut(s) 99, 434, 611
ApoI RAATTY 2 cut(s) 193, 365
AspLEI GCGC 2 cut(s) 286, 654
AspS9I GGNCC 4 cut(s) 200, 355, 640, 697
AsuC2I CCSGG 1 cut(s) 288
AvaII GGWCC 2 cut(s) 640, 697
BanII GRGCYC 1 cut(s) 294
BbsI GAAGAC 1 cut(s) 519
BbvCI CCTCAGC 1 cut(s) 589
BbvI GCAGC 3 cut(s) 111, 421, 598
BccI CCATC 5 cut(s) 112, 142, 148, 160, 166
BceAI ACGGC 1 cut(s) 696
BciT130I CCWGG 1 cut(s) 828
BcnI CCSGG 1 cut(s) 288
BcoDI GTCTC 1 cut(s) 32
BfmI CTRYAG 1 cut(s) 130
BfoI RGCGCY 1 cut(s) 655
BisI GCNGC 3 cut(s) 100, 435, 612
BlsI GCNGC 3 cut(s) 101, 436, 613
BmcAI AGTACT 1 cut(s) 385
Bme1390I CCNGG 2 cut(s) 288, 828
Bme18I GGWCC 2 cut(s) 640, 697
BmgT120I GGNCC 4 cut(s) 200, 355, 640, 697
BmiI GGNNCC 1 cut(s) 201
BmrFI CCNGG 2 cut(s) 288, 828
BmsI GCATC 2 cut(s) 618, 640
BpiI GAAGAC 1 cut(s) 519
BplI GAGNNNNNCTC 2 cut(s) 252, 284
BpmI CTGGAG 1 cut(s) 105
Bpu10I CCTNAGC 2 cut(s) 589, 708
BpuEI CTTGAG 2 cut(s) 278, 493
BpuMI CCSGG 1 cut(s) 288
BsaAI YACGTR 1 cut(s) 744
BsaJI CCNNGG 2 cut(s) 142, 287
BsaXI ACNNNNNCTCC 2 cut(s) 323, 353
Bsc4I CCNNNNNNNGG 6 cut(s) 21, 148, 197, 288, 523, 783
Bse1I ACTGG 2 cut(s) 79, 561
BseBI CCWGG 1 cut(s) 828
BseDI CCNNGG 2 cut(s) 142, 287
BseGI GGATG 7 cut(s) 153, 159, 171, 177, 183, 492, 566
BseLI CCNNNNNNNGG 6 cut(s) 21, 148, 197, 288, 523, 783
BseMII CTCAG 2 cut(s) 603, 722
BseNI ACTGG 2 cut(s) 79, 561
BseRI GAGGAG 2 cut(s) 347, 365
BseXI GCAGC 3 cut(s) 111, 421, 598
Bsh1236I CGCG 1 cut(s) 284
BshFI GGCC 4 cut(s) 202, 357, 683, 831
BsiSI CCGG 1 cut(s) 287
BslFI GGGAC 1 cut(s) 691
BslI CCNNNNNNNGG 6 cut(s) 21, 148, 197, 288, 523, 783
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 1 cut(s) 691
BsnI GGCC 4 cut(s) 202, 357, 683, 831
Bsp1286I GDGCHC 1 cut(s) 294
Bsp1407I TGTACA 1 cut(s) 779
Bsp143I GATC 1 cut(s) 874
Bsp19I CCATGG 1 cut(s) 142
BspACI CCGC 3 cut(s) 282, 499, 851
BspANI GGCC 4 cut(s) 202, 357, 683, 831
BspCNI CTCAG 2 cut(s) 602, 721
BspFNI CGCG 1 cut(s) 284
BspLI GGNNCC 1 cut(s) 201
BspPI GGATC 1 cut(s) 882
BsrGI TGTACA 1 cut(s) 779
BsrI ACTGG 2 cut(s) 79, 561
BssECI CCNNGG 2 cut(s) 142, 287
BssMI GATC 1 cut(s) 874
BssT1I CCWWGG 1 cut(s) 142
Bst2UI CCWGG 1 cut(s) 828
Bst4CI ACNGT 3 cut(s) 134, 227, 457
BstAUI TGTACA 1 cut(s) 779
BstBAI YACGTR 1 cut(s) 744
BstC8I GCNNGC 2 cut(s) 204, 656
BstDEI CTNAG 3 cut(s) 589, 708, 909
BstDSI CCRYGG 1 cut(s) 142
BstENI CCTNNNNNAGG 1 cut(s) 781
BstF5I GGATG 7 cut(s) 153, 159, 171, 177, 183, 492, 566
BstFNI CGCG 1 cut(s) 284
BstH2I RGCGCY 1 cut(s) 655
BstHHI GCGC 2 cut(s) 286, 654
BstKTI GATC 1 cut(s) 877
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 1 cut(s) 874
BstMWI GCNNNNNNNGC 1 cut(s) 837
BstNI CCWGG 1 cut(s) 828
BstSCI CCNGG 2 cut(s) 286, 826
BstSFI CTRYAG 1 cut(s) 130
BstSNI TACGTA 1 cut(s) 744
BstUI CGCG 1 cut(s) 284
BstV1I GCAGC 3 cut(s) 111, 421, 598
BstV2I GAAGAC 1 cut(s) 519
BstX2I RGATCY 1 cut(s) 874
BstXI CCANNNNNNTGG 1 cut(s) 214
BstYI RGATCY 1 cut(s) 874
BsuRI GGCC 4 cut(s) 202, 357, 683, 831
BtgI CCRYGG 1 cut(s) 142
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 7 cut(s) 153, 159, 171, 177, 183, 492, 566
BtsI GCAGTG 1 cut(s) 665
BtsIMutI CAGTG 2 cut(s) 139, 665
Cac8I GCNNGC 2 cut(s) 204, 656
CfoI GCGC 2 cut(s) 286, 654
Cfr13I GGNCC 4 cut(s) 200, 355, 640, 697
Csp6I GTAC 5 cut(s) 340, 384, 421, 745, 780
CviAII CATG 6 cut(s) 32, 143, 208, 214, 299, 814
CviQI GTAC 5 cut(s) 340, 384, 421, 745, 780
DdeI CTNAG 3 cut(s) 589, 708, 909
DpnI GATC 1 cut(s) 876
DpnII GATC 1 cut(s) 874
EaeI YGGCCR 1 cut(s) 681
Eco105I TACGTA 1 cut(s) 744
Eco130I CCWWGG 1 cut(s) 142
Eco24I GRGCYC 1 cut(s) 294
Eco47I GGWCC 2 cut(s) 640, 697
Eco47III AGCGCT 1 cut(s) 653
Eco57I CTGAAG 1 cut(s) 531
EcoNI CCTNNNNNAGG 1 cut(s) 781
EcoO109I RGGNCCY 1 cut(s) 355
EcoRI GAATTC 1 cut(s) 193
EcoRII CCWGG 1 cut(s) 826
EcoT14I CCWWGG 1 cut(s) 142
EcoT22I ATGCAT 2 cut(s) 633, 866
EcoT38I GRGCYC 1 cut(s) 294
ErhI CCWWGG 1 cut(s) 142
FaeI CATG 6 cut(s) 35, 146, 211, 217, 302, 817
FaqI GGGAC 1 cut(s) 691
FatI CATG 6 cut(s) 31, 142, 207, 213, 298, 813
FauI CCCGC 1 cut(s) 858
Fnu4HI GCNGC 3 cut(s) 100, 435, 612
FokI GGATG 7 cut(s) 160, 166, 178, 184, 190, 499, 553
FriOI GRGCYC 1 cut(s) 294
Fsp4HI GCNGC 3 cut(s) 100, 435, 612
GlaI GCGC 2 cut(s) 285, 653
GluI GCNGC 3 cut(s) 100, 435, 612
GsuI CTGGAG 1 cut(s) 105
HaeII RGCGCY 1 cut(s) 655
HaeIII GGCC 4 cut(s) 202, 357, 683, 831
HapII CCGG 1 cut(s) 287
HhaI GCGC 2 cut(s) 286, 654
Hin1II CATG 6 cut(s) 35, 146, 211, 217, 302, 817
Hin6I GCGC 2 cut(s) 284, 652
HinP1I GCGC 2 cut(s) 284, 652
HincII GTYRAC 2 cut(s) 220, 242
HindII GTYRAC 2 cut(s) 220, 242
HinfI GANTC 2 cut(s) 278, 563
HpaII CCGG 1 cut(s) 287
Hpy166II GTNNAC 3 cut(s) 220, 242, 250
Hpy188I TCNGA 4 cut(s) 106, 117, 192, 546
Hpy188III TCNNGA 4 cut(s) 84, 258, 275, 805
Hpy8I GTNNAC 3 cut(s) 220, 242, 250
HpyAV CCTTC 4 cut(s) 281, 526, 541, 659
HpyCH4III ACNGT 3 cut(s) 134, 227, 457
HpyCH4IV ACGT 2 cut(s) 743, 858
HpyCH4V TGCA 6 cut(s) 10, 31, 437, 631, 658, 864
HpyF10VI GCNNNNNNNGC 1 cut(s) 837
HpyF3I CTNAG 3 cut(s) 589, 708, 909
HpySE526I ACGT 2 cut(s) 743, 858
Hsp92II CATG 6 cut(s) 35, 146, 211, 217, 302, 817
HspAI GCGC 2 cut(s) 284, 652
Kzo9I GATC 1 cut(s) 874
LmnI GCTCC 4 cut(s) 86, 260, 334, 598
Lsp1109I GCAGC 3 cut(s) 111, 421, 598
LweI GCATC 2 cut(s) 618, 640
MaeII ACGT 2 cut(s) 743, 858
MaeIII GTNAC 3 cut(s) 125, 750, 889
MalI GATC 1 cut(s) 876
MboI GATC 1 cut(s) 874
MboII GAAGA 1 cut(s) 524
MflI RGATCY 1 cut(s) 874
MhlI GDGCHC 1 cut(s) 294
MluCI AATT 4 cut(s) 193, 234, 344, 365
MmeI TCCRAC 1 cut(s) 39
Mph1103I ATGCAT 2 cut(s) 633, 866
MseI TTAA 2 cut(s) 717, 794
MslI CAYNNNNRTG 1 cut(s) 212
MspA1I CMGCKG 1 cut(s) 853
MspI CCGG 1 cut(s) 287
MspR9I CCNGG 2 cut(s) 288, 828
MvaI CCWGG 1 cut(s) 828
MvnI CGCG 1 cut(s) 284
MwoI GCNNNNNNNGC 1 cut(s) 837
NciI CCSGG 1 cut(s) 288
NcoI CCATGG 1 cut(s) 142
NdeII GATC 1 cut(s) 874
NlaIII CATG 6 cut(s) 35, 146, 211, 217, 302, 817
NlaIV GGNNCC 1 cut(s) 201
NsiI ATGCAT 2 cut(s) 633, 866
PfeI GAWTC 2 cut(s) 278, 563
PflMI CCANNNNNTGG 1 cut(s) 148
PkrI GCNGC 3 cut(s) 101, 436, 613
Ppu21I YACGTR 1 cut(s) 744
Psp6I CCWGG 1 cut(s) 826
PspGI CCWGG 1 cut(s) 826
PspN4I GGNNCC 1 cut(s) 201
PspPI GGNCC 4 cut(s) 200, 355, 640, 697
PsuI RGATCY 1 cut(s) 874
RsaI GTAC 5 cut(s) 341, 385, 422, 746, 781
RsaNI GTAC 5 cut(s) 340, 384, 421, 745, 780
RseI CAYNNNNRTG 1 cut(s) 212
SaqAI TTAA 2 cut(s) 717, 794
SatI GCNGC 3 cut(s) 100, 435, 612
Sau3AI GATC 1 cut(s) 874
Sau96I GGNCC 4 cut(s) 200, 355, 640, 697
ScaI AGTACT 1 cut(s) 385
ScrFI CCNGG 2 cut(s) 288, 828
SduI GDGCHC 1 cut(s) 294
SfaNI GCATC 2 cut(s) 618, 640
SfcI CTRYAG 1 cut(s) 130
SinI GGWCC 2 cut(s) 640, 697
SmiMI CAYNNNNRTG 1 cut(s) 212
SmlI CTYRAG 2 cut(s) 293, 472
SmoI CTYRAG 2 cut(s) 293, 472
SnaBI TACGTA 1 cut(s) 744
Sse9I AATT 4 cut(s) 193, 234, 344, 365
SsiI CCGC 3 cut(s) 282, 499, 851
StyD4I CCNGG 2 cut(s) 286, 826
StyI CCWWGG 1 cut(s) 142
TaaI ACNGT 3 cut(s) 134, 227, 457
TaiI ACGT 2 cut(s) 746, 861
TaqI TCGA 2 cut(s) 318, 634
TasI AATT 4 cut(s) 193, 234, 344, 365
TatI WGTACW 3 cut(s) 339, 383, 779
TfiI GAWTC 2 cut(s) 278, 563
Tru1I TTAA 2 cut(s) 717, 794
Tru9I TTAA 2 cut(s) 717, 794
TscAI CASTG 2 cut(s) 139, 665
TseI GCWGC 3 cut(s) 99, 434, 611
TspDTI ATGAA 2 cut(s) 390, 417
TspRI CASTG 2 cut(s) 139, 665
Van91I CCANNNNNTGG 1 cut(s) 148
VpaK11BI GGWCC 2 cut(s) 640, 697
XagI CCTNNNNNAGG 1 cut(s) 781
XapI RAATTY 2 cut(s) 193, 365
XcmI CCANNNNNNNNNTGG 1 cut(s) 691
ZrmI AGTACT 1 cut(s) 385
Zsp2I ATGCAT 2 cut(s) 633, 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.