RchiOBHm_Chr4g0446241

Protein of unknown function (DUF3755)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004772
Physical Location & Seq
Forward (+)
66493583 .. 66498825
5243 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41376

Sequence Viewer

Length: 849 bp
ATGGCGATGGAGTCGAACACCGGGTTTCTACATGATGACACTTTTGGCTATGGCTTGAACCGGCACGCTATATCTTTCCAGTCTGGAGCAATAAACAGCAGCTCGGAGATGATTCCGATGGGGAATTACTTTAGTACTGACCCGGTGATGGGGATGGGGATGGGGATGCAGATGATGTTCTCTCCGACTTCGGGGTCAGGCATGGGCATGGGCATGGTCAACCACAGTCCTGCAATTAGTCAACCCGGGACTTCATCAGGGTCTTCTCTGCTCGTTGATTCCGTACCGGGCCTCAAGCATGACACAGGCTTGGCAGTTGAGTGGTCTTTGGAGGAGCAATACAAATTGGAGGAGGGCCTTGTCAAATATGCTGATGAACCGAGTATTATGAGGTACATAAAGATTGCGGCAACCTTGCGTGATAAAACTGTGCGTGATGTTGCCTTGAGGTGTAGGTGGATGACGAGAAAGCGGAGGAAACCTGAAGACCATACTACAGGAAAGAAGGGCAACATTAGGAAGGATAAACTGGTAGATTCATACTCCAAGATGAGCATACCCTCAGCTCCACAACACAACATGGCTGCATATTCTCTTATGATGCATCGTATGAACCAGAATGAGCGTTTGCAGTGTGAAGGAATAAGTGGGACAGCCAAGCATTTATTGGACCAAAATGCTCAAGCTTTTAATCAAATCACAGCTAACCTTTCTACGTACAAGTTACAGGACAATATTAACCTCTTCTGTCGCACAAGGAACAACTTAACTGCCATCTTGAATGAGTATCATGAGAGGTATGCCTGGCTTAATGAGCCAAATGCCGCCACTGGATGTATGCATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3755

Protein Analysis

282

Amino Acids

31.5

Weight (kDa)

8.59

Isoelectric Point (pI)

47.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3755 PF12579 239 - 262 3.8e-11 Protein of unknown function (DUF3755)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 193
AciI CCGC 3 cut(s) 407, 472, 825
AcuI CTGAAG 1 cut(s) 504
AfaI GTAC 4 cut(s) 136, 285, 395, 719
AfiI CCNNNNNNNGG 3 cut(s) 148, 149, 191
AgsI TTSAA 2 cut(s) 58, 781
AjnI CCWGG 1 cut(s) 803
AluBI AGCT 4 cut(s) 102, 566, 686, 704
AluI AGCT 4 cut(s) 102, 566, 686, 704
Ama87I CYCGRG 1 cut(s) 245
AoxI GGCC 2 cut(s) 289, 355
ApeKI GCWGC 2 cut(s) 99, 584
AspS9I GGNCC 3 cut(s) 289, 355, 670
AsuC2I CCSGG 5 cut(s) 22, 143, 246, 247, 288
AsuHPI GGTGA 1 cut(s) 157
AvaI CYCGRG 1 cut(s) 245
AvaII GGWCC 1 cut(s) 670
BbsI GAAGAC 2 cut(s) 255, 492
BbvCI CCTCAGC 1 cut(s) 562
BbvI GCAGC 2 cut(s) 111, 571
BccI CCATC 5 cut(s) 112, 142, 148, 154, 782
BciT130I CCWGG 1 cut(s) 805
BcnI CCSGG 5 cut(s) 22, 143, 246, 247, 288
BfmI CTRYAG 1 cut(s) 495
BisI GCNGC 4 cut(s) 100, 408, 585, 825
BlsI GCNGC 4 cut(s) 101, 409, 586, 826
BmcAI AGTACT 1 cut(s) 136
Bme1390I CCNGG 6 cut(s) 22, 143, 246, 247, 288, 805
Bme18I GGWCC 1 cut(s) 670
BmeT110I CYCGRG 1 cut(s) 245
BmgT120I GGNCC 3 cut(s) 289, 355, 670
BmrFI CCNGG 6 cut(s) 22, 143, 246, 247, 288, 805
BmsI GCATC 3 cut(s) 156, 591, 613
BpiI GAAGAC 2 cut(s) 255, 492
BpmI CTGGAG 1 cut(s) 105
Bpu10I CCTNAGC 1 cut(s) 562
BpuEI CTTGAG 3 cut(s) 278, 466, 666
BpuMI CCSGG 5 cut(s) 22, 143, 246, 247, 288
BsaAI YACGTR 1 cut(s) 717
BsaJI CCNNGG 1 cut(s) 245
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 149, 191
Bse118I RCCGGY 1 cut(s) 60
Bse1I ACTGG 3 cut(s) 79, 534, 835
BseBI CCWGG 1 cut(s) 805
BseDI CCNNGG 1 cut(s) 245
BseGI GGATG 5 cut(s) 159, 165, 171, 465, 839
BseLI CCNNNNNNNGG 3 cut(s) 148, 149, 191
BseMII CTCAG 1 cut(s) 576
BseNI ACTGG 3 cut(s) 79, 534, 835
BseRI GAGGAG 2 cut(s) 347, 365
BseXI GCAGC 2 cut(s) 111, 571
BshFI GGCC 2 cut(s) 291, 357
BsiHKCI CYCGRG 1 cut(s) 245
BsiSI CCGG 5 cut(s) 21, 61, 143, 246, 287
BslFI GGGAC 2 cut(s) 262, 664
BslI CCNNNNNNNGG 3 cut(s) 148, 149, 191
BsmFI GGGAC 2 cut(s) 262, 664
BsnI GGCC 2 cut(s) 291, 357
BsoBI CYCGRG 1 cut(s) 245
BspACI CCGC 3 cut(s) 407, 472, 825
BspANI GGCC 2 cut(s) 291, 357
BspCNI CTCAG 1 cut(s) 575
BspHI TCATGA 1 cut(s) 790
BsrFI RCCGGY 1 cut(s) 60
BsrI ACTGG 3 cut(s) 79, 534, 835
BssAI RCCGGY 1 cut(s) 60
BssECI CCNNGG 1 cut(s) 245
Bst2UI CCWGG 1 cut(s) 805
Bst4CI ACNGT 2 cut(s) 227, 430
Bst6I CTCTTC 1 cut(s) 749
BstBAI YACGTR 1 cut(s) 717
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 1 cut(s) 562
BstF5I GGATG 5 cut(s) 159, 165, 171, 465, 839
BstMWI GCNNNNNNNGC 1 cut(s) 814
BstNI CCWGG 1 cut(s) 805
BstSCI CCNGG 6 cut(s) 20, 141, 244, 245, 286, 803
BstSFI CTRYAG 1 cut(s) 495
BstSNI TACGTA 1 cut(s) 717
BstV1I GCAGC 2 cut(s) 111, 571
BstV2I GAAGAC 2 cut(s) 255, 492
BsuRI GGCC 2 cut(s) 291, 357
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 5 cut(s) 159, 165, 171, 465, 839
BtsI GCAGTG 1 cut(s) 638
BtsIMutI CAGTG 2 cut(s) 638, 828
Cac8I GCNNGC 1 cut(s) 66
CciI TCATGA 1 cut(s) 790
Cfr10I RCCGGY 1 cut(s) 60
Cfr13I GGNCC 3 cut(s) 289, 355, 670
Cfr9I CCCGGG 1 cut(s) 245
Csp6I GTAC 4 cut(s) 135, 284, 394, 718
CviAII CATG 7 cut(s) 32, 202, 208, 214, 299, 580, 791
CviQI GTAC 4 cut(s) 135, 284, 394, 718
DdeI CTNAG 1 cut(s) 562
DrdI GACNNNNNNGTC 1 cut(s) 193
DseDI GACNNNNNNGTC 1 cut(s) 193
Eam1104I CTCTTC 1 cut(s) 749
EarI CTCTTC 1 cut(s) 749
Eco105I TACGTA 1 cut(s) 717
Eco47I GGWCC 1 cut(s) 670
Eco57I CTGAAG 1 cut(s) 504
Eco88I CYCGRG 1 cut(s) 245
EcoO109I RGGNCCY 1 cut(s) 355
EcoRII CCWGG 1 cut(s) 803
EcoT22I ATGCAT 2 cut(s) 606, 843
FaeI CATG 7 cut(s) 35, 205, 211, 217, 302, 583, 794
FaqI GGGAC 2 cut(s) 262, 664
FatI CATG 7 cut(s) 31, 201, 207, 213, 298, 579, 790
Fnu4HI GCNGC 4 cut(s) 100, 408, 585, 825
FokI GGATG 4 cut(s) 166, 172, 178, 472
Fsp4HI GCNGC 4 cut(s) 100, 408, 585, 825
GluI GCNGC 4 cut(s) 100, 408, 585, 825
GsuI CTGGAG 1 cut(s) 105
HaeIII GGCC 2 cut(s) 291, 357
HapII CCGG 5 cut(s) 21, 61, 143, 246, 287
Hin1II CATG 7 cut(s) 35, 205, 211, 217, 302, 583, 794
HincII GTYRAC 2 cut(s) 220, 242
HindII GTYRAC 2 cut(s) 220, 242
HindIII AAGCTT 1 cut(s) 684
HinfI GANTC 4 cut(s) 11, 112, 278, 536
HpaII CCGG 5 cut(s) 21, 61, 143, 246, 287
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 2 cut(s) 220, 242
Hpy188I TCNGA 3 cut(s) 106, 117, 186
Hpy188III TCNNGA 3 cut(s) 84, 778, 791
Hpy8I GTNNAC 2 cut(s) 220, 242
HpyAV CCTTC 3 cut(s) 499, 514, 632
HpyCH4III ACNGT 2 cut(s) 227, 430
HpyCH4IV ACGT 1 cut(s) 716
HpyCH4V TGCA 6 cut(s) 169, 233, 587, 604, 631, 841
HpyF10VI GCNNNNNNNGC 1 cut(s) 814
HpyF3I CTNAG 1 cut(s) 562
HpySE526I ACGT 1 cut(s) 716
Hsp92II CATG 7 cut(s) 35, 205, 211, 217, 302, 583, 794
LmnI GCTCC 3 cut(s) 86, 334, 571
Lsp1109I GCAGC 2 cut(s) 111, 571
LweI GCATC 3 cut(s) 156, 591, 613
MaeII ACGT 1 cut(s) 716
MaeIII GTNAC 1 cut(s) 723
MboII GAAGA 3 cut(s) 255, 497, 736
MluCI AATT 3 cut(s) 124, 234, 344
MlyI GAGTC 1 cut(s) 20
MmeI TCCRAC 1 cut(s) 209
Mph1103I ATGCAT 2 cut(s) 606, 843
MseI TTAA 4 cut(s) 690, 738, 767, 810
MslI CAYNNNNRTG 2 cut(s) 206, 212
MspI CCGG 5 cut(s) 21, 61, 143, 246, 287
MspR9I CCNGG 6 cut(s) 22, 143, 246, 247, 288, 805
MvaI CCWGG 1 cut(s) 805
MwoI GCNNNNNNNGC 1 cut(s) 814
NciI CCSGG 5 cut(s) 22, 143, 246, 247, 288
NlaIII CATG 7 cut(s) 35, 205, 211, 217, 302, 583, 794
NsiI ATGCAT 2 cut(s) 606, 843
PagI TCATGA 1 cut(s) 790
PcsI WCGNNNNNNNCGW 1 cut(s) 279
PfeI GAWTC 3 cut(s) 112, 278, 536
PkrI GCNGC 4 cut(s) 101, 409, 586, 826
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
Ppu21I YACGTR 1 cut(s) 717
Psp6I CCWGG 1 cut(s) 803
PspGI CCWGG 1 cut(s) 803
PspPI GGNCC 3 cut(s) 289, 355, 670
RsaI GTAC 4 cut(s) 136, 285, 395, 719
RsaNI GTAC 4 cut(s) 135, 284, 394, 718
RseI CAYNNNNRTG 2 cut(s) 206, 212
SaqAI TTAA 4 cut(s) 690, 738, 767, 810
SatI GCNGC 4 cut(s) 100, 408, 585, 825
Sau96I GGNCC 3 cut(s) 289, 355, 670
ScaI AGTACT 1 cut(s) 136
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 6 cut(s) 22, 143, 246, 247, 288, 805
SfaNI GCATC 3 cut(s) 156, 591, 613
SfcI CTRYAG 1 cut(s) 495
SinI GGWCC 1 cut(s) 670
SmaI CCCGGG 1 cut(s) 247
SmiMI CAYNNNNRTG 2 cut(s) 206, 212
SmlI CTYRAG 3 cut(s) 293, 445, 681
SmoI CTYRAG 3 cut(s) 293, 445, 681
SnaBI TACGTA 1 cut(s) 717
Sse9I AATT 3 cut(s) 124, 234, 344
SsiI CCGC 3 cut(s) 407, 472, 825
SspI AATATT 1 cut(s) 736
StyD4I CCNGG 6 cut(s) 20, 141, 244, 245, 286, 803
TaaI ACNGT 2 cut(s) 227, 430
TaiI ACGT 1 cut(s) 719
TaqI TCGA 1 cut(s) 14
TasI AATT 3 cut(s) 124, 234, 344
TatI WGTACW 1 cut(s) 134
TauI GCSGC 2 cut(s) 410, 827
TfiI GAWTC 3 cut(s) 112, 278, 536
Tru1I TTAA 4 cut(s) 690, 738, 767, 810
Tru9I TTAA 4 cut(s) 690, 738, 767, 810
TscAI CASTG 2 cut(s) 638, 835
TseI GCWGC 2 cut(s) 99, 584
TspDTI ATGAA 4 cut(s) 243, 390, 528, 626
TspGWI ACGGA 1 cut(s) 271
TspMI CCCGGG 1 cut(s) 245
TspRI CASTG 2 cut(s) 638, 835
VpaK11BI GGWCC 1 cut(s) 670
XcmI CCANNNNNNNNNTGG 1 cut(s) 664
XmaI CCCGGG 1 cut(s) 245
ZrmI AGTACT 1 cut(s) 136
Zsp2I ATGCAT 2 cut(s) 606, 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.