MD16G1006900.v1.1

Protein of unknown function (DUF3755)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004772
Physical Location & Seq
Reverse (-)
527610 .. 531236
3627 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1006900.v1.1.491

Sequence Viewer

Length: 834 bp
ATGGCGATGCAGTCGAACACAGGGTTTCACCGTGACGAAGCTTTTGGCTACGGTTTGAACCGACACGCGATATCGTTTCAGTCCGGGGCCATAAACAGCAGTTCGGAGATGATTCCGATGGGGAATTACTTTGGTACGGACGGGGGCATGATGTTTTCGTCGCATTCGGGCATAGTTAACAACAATCCTGTAATTAGTCAGGCTGGGAGTTCATCTGGTTCTCTGCTTCTCGATTCGGTCCCTGGGCTCAAGCATGACACAGGTTTGGCTGTTGAGTGGTCTGTGGAGGAACAGTACAAATTGGAGGAGGGCCTTGGCAAATATGCCAATGAACCAAGTATTATGAGGTACATAAAGATCGCGGCAATGTTACGTGATAAAACTGTGCGTGATGTTGCTTTGAGGTGTAGGTGGATGACTAGAAAGCGTAGGAAATCTGAAGAACATAGTATGGGAAAGAAGGGGAACATTAGGAAGGATAAACTGGTGGAGTCGTCTTCAAAGACAAACATACATTCAGCTCCACCACTGGACATGGCTGCATATTCTCTTATGGGGCACCATATGGACCAGAATGAGCGTTTGCAGTATGAAGGATTAAGTGGCACAGCCAAGCATCTATTGGAGCAAAATGCTCAAGCTTTCAGTCAAATTACATCTAATCTTTCTTCATACAAGTTACAGGAAAACATTGACCTCTTCTACCGCACGAGGAACAATATAACTGCCATCCTTAATGACATGAGAGCTATGCCTGGCATAATGAGCCAAATGCCACCACTGCCACCCATTGACGAGGATCTTGCTAATAGTATCTTGCGTAATGCGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3755

Protein Analysis

278

Amino Acids

30.82

Weight (kDa)

7.87

Isoelectric Point (pI)

52.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3755 PF12579 224 - 257 4.9e-19 Protein of unknown function (DUF3755)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 558
AccII CGCG 2 cut(s) 68, 362
AciI CCGC 2 cut(s) 362, 706
AclWI GGATC 1 cut(s) 807
AcuI CTGAAG 1 cut(s) 459
AfaI GTAC 3 cut(s) 136, 296, 350
AgsI TTSAA 2 cut(s) 58, 501
AjnI CCWGG 2 cut(s) 241, 754
AluBI AGCT 4 cut(s) 41, 521, 641, 749
AluI AGCT 4 cut(s) 41, 521, 641, 749
AlwI GGATC 1 cut(s) 807
AoxI GGCC 2 cut(s) 87, 310
ApeKI GCWGC 1 cut(s) 539
ArsI GACNNNNNNTTYG 2 cut(s) 26, 58
AspS9I GGNCC 4 cut(s) 87, 238, 310, 568
AsuC2I CCSGG 1 cut(s) 85
AsuHPI GGTGA 1 cut(s) 20
AvaII GGWCC 2 cut(s) 238, 568
BaeGI GKGCMC 1 cut(s) 561
BanI GGYRCC 1 cut(s) 558
BanII GRGCYC 1 cut(s) 249
BauI CACGAG 1 cut(s) 709
BbsI GAAGAC 1 cut(s) 489
BbvI GCAGC 1 cut(s) 526
BccI CCATC 2 cut(s) 112, 737
BcgI CGANNNNNNTGC 2 cut(s) 785, 819
BciT130I CCWGG 2 cut(s) 243, 756
BcnI CCSGG 1 cut(s) 85
BfaI CTAG 1 cut(s) 420
BisI GCNGC 2 cut(s) 363, 540
BlsI GCNGC 2 cut(s) 364, 541
Bme1390I CCNGG 3 cut(s) 85, 243, 756
Bme18I GGWCC 2 cut(s) 238, 568
BmgT120I GGNCC 4 cut(s) 87, 238, 310, 568
BmiI GGNNCC 3 cut(s) 88, 240, 560
BmrFI CCNGG 3 cut(s) 85, 243, 756
BmsI GCATC 1 cut(s) 625
BpiI GAAGAC 1 cut(s) 489
BpuEI CTTGAG 2 cut(s) 233, 621
BpuMI CCSGG 1 cut(s) 85
BsaAI YACGTR 1 cut(s) 374
BsaJI CCNNGG 4 cut(s) 84, 241, 242, 313
BsaXI ACNNNNNCTCC 2 cut(s) 278, 308
Bse1I ACTGG 2 cut(s) 489, 534
Bse3DI GCAATG 1 cut(s) 372
BseBI CCWGG 2 cut(s) 243, 756
BseDI CCNNGG 4 cut(s) 84, 241, 242, 313
BseGI GGATG 2 cut(s) 420, 729
BseMI GCAATG 1 cut(s) 372
BseNI ACTGG 2 cut(s) 489, 534
BseRI GAGGAG 1 cut(s) 320
BseSI GKGCMC 1 cut(s) 561
BseXI GCAGC 1 cut(s) 526
BseYI CCCAGC 1 cut(s) 203
Bsh1236I CGCG 2 cut(s) 68, 362
BshFI GGCC 2 cut(s) 89, 312
BshNI GGYRCC 1 cut(s) 558
BsiSI CCGG 1 cut(s) 84
BslFI GGGAC 1 cut(s) 224
BsmFI GGGAC 1 cut(s) 224
BsmI GAATGC 1 cut(s) 163
BsnI GGCC 2 cut(s) 89, 312
Bsp1286I GDGCHC 2 cut(s) 249, 561
Bsp143I GATC 2 cut(s) 357, 799
BspACI CCGC 2 cut(s) 362, 706
BspANI GGCC 2 cut(s) 89, 312
BspFNI CGCG 2 cut(s) 68, 362
BspLI GGNNCC 3 cut(s) 88, 240, 560
BspPI GGATC 1 cut(s) 807
BspT107I GGYRCC 1 cut(s) 558
BsrDI GCAATG 1 cut(s) 372
BsrI ACTGG 2 cut(s) 489, 534
BssECI CCNNGG 4 cut(s) 84, 241, 242, 313
BssMI GATC 2 cut(s) 357, 799
BssSI CACGAG 1 cut(s) 709
BssT1I CCWWGG 1 cut(s) 313
Bst2BI CACGAG 1 cut(s) 709
Bst2UI CCWGG 2 cut(s) 243, 756
Bst4CI ACNGT 4 cut(s) 32, 53, 294, 385
Bst6I CTCTTC 1 cut(s) 704
BstBAI YACGTR 1 cut(s) 374
BstDEI CTNAG 1 cut(s) 831
BstF5I GGATG 2 cut(s) 420, 729
BstFNI CGCG 2 cut(s) 68, 362
BstKTI GATC 2 cut(s) 360, 802
BstMBI GATC 2 cut(s) 357, 799
BstMWI GCNNNNNNNGC 2 cut(s) 765, 781
BstNI CCWGG 2 cut(s) 243, 756
BstSCI CCNGG 3 cut(s) 83, 241, 754
BstSLI GKGCMC 1 cut(s) 561
BstUI CGCG 2 cut(s) 68, 362
BstV1I GCAGC 1 cut(s) 526
BstV2I GAAGAC 1 cut(s) 489
BstX2I RGATCY 1 cut(s) 799
BstYI RGATCY 1 cut(s) 799
BsuRI GGCC 2 cut(s) 89, 312
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 2 cut(s) 420, 729
BtsI GCAGTG 1 cut(s) 779
BtsIMutI CAGTG 2 cut(s) 527, 779
Cfr13I GGNCC 4 cut(s) 87, 238, 310, 568
Csp6I GTAC 3 cut(s) 135, 295, 349
CviAII CATG 4 cut(s) 148, 254, 535, 742
CviQI GTAC 3 cut(s) 135, 295, 349
DdeI CTNAG 1 cut(s) 831
DpnI GATC 2 cut(s) 359, 801
DpnII GATC 2 cut(s) 357, 799
Eam1104I CTCTTC 1 cut(s) 704
EarI CTCTTC 1 cut(s) 704
Eco130I CCWWGG 1 cut(s) 313
Eco24I GRGCYC 1 cut(s) 249
Eco32I GATATC 1 cut(s) 72
Eco47I GGWCC 2 cut(s) 238, 568
Eco57I CTGAAG 1 cut(s) 459
EcoO109I RGGNCCY 1 cut(s) 310
EcoRII CCWGG 2 cut(s) 241, 754
EcoRV GATATC 1 cut(s) 72
EcoT14I CCWWGG 1 cut(s) 313
EcoT38I GRGCYC 1 cut(s) 249
ErhI CCWWGG 1 cut(s) 313
FaeI CATG 4 cut(s) 151, 257, 538, 745
FaqI GGGAC 1 cut(s) 224
FatI CATG 4 cut(s) 147, 253, 534, 741
FauNDI CATATG 1 cut(s) 564
Fnu4HI GCNGC 2 cut(s) 363, 540
FokI GGATG 2 cut(s) 427, 716
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 2 cut(s) 363, 540
FspBI CTAG 1 cut(s) 420
GluI GCNGC 2 cut(s) 363, 540
GsaI CCCAGC 1 cut(s) 207
HaeIII GGCC 2 cut(s) 89, 312
HapII CCGG 1 cut(s) 84
Hin1II CATG 4 cut(s) 151, 257, 538, 745
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HindIII AAGCTT 2 cut(s) 39, 639
HinfI GANTC 3 cut(s) 112, 233, 491
HpaI GTTAAC 1 cut(s) 178
HpaII CCGG 1 cut(s) 84
HphI GGTGA 1 cut(s) 20
Hpy166II GTNNAC 1 cut(s) 178
Hpy188I TCNGA 3 cut(s) 106, 117, 439
Hpy188III TCNNGA 1 cut(s) 230
Hpy8I GTNNAC 1 cut(s) 178
Hpy99I CGWCG 1 cut(s) 163
HpyAV CCTTC 3 cut(s) 454, 469, 587
HpyCH4III ACNGT 4 cut(s) 32, 53, 294, 385
HpyCH4IV ACGT 1 cut(s) 373
HpyCH4V TGCA 3 cut(s) 10, 542, 586
HpyF10VI GCNNNNNNNGC 2 cut(s) 765, 781
HpyF3I CTNAG 1 cut(s) 831
HpySE526I ACGT 1 cut(s) 373
Hsp92II CATG 4 cut(s) 151, 257, 538, 745
KspAI GTTAAC 1 cut(s) 178
Kzo9I GATC 2 cut(s) 357, 799
LmnI GCTCC 2 cut(s) 526, 625
Lsp1109I GCAGC 1 cut(s) 526
LweI GCATC 1 cut(s) 625
MaeI CTAG 1 cut(s) 420
MaeII ACGT 1 cut(s) 373
MaeIII GTNAC 3 cut(s) 32, 369, 678
MalI GATC 2 cut(s) 359, 801
MboI GATC 2 cut(s) 357, 799
MboII GAAGA 4 cut(s) 452, 489, 660, 691
MflI RGATCY 1 cut(s) 799
MhlI GDGCHC 2 cut(s) 249, 561
MluCI AATT 4 cut(s) 124, 192, 299, 651
MlyI GAGTC 1 cut(s) 500
MnlI CCTC 8 cut(s) 280, 298, 301, 339, 396, 705, 707, 790
MseI TTAA 3 cut(s) 177, 599, 735
MspI CCGG 1 cut(s) 84
MspR9I CCNGG 3 cut(s) 85, 243, 756
Mva1269I GAATGC 1 cut(s) 163
MvaI CCWGG 2 cut(s) 243, 756
MvnI CGCG 2 cut(s) 68, 362
MwoI GCNNNNNNNGC 2 cut(s) 765, 781
NciI CCSGG 1 cut(s) 85
NdeI CATATG 1 cut(s) 564
NdeII GATC 2 cut(s) 357, 799
NlaIII CATG 4 cut(s) 151, 257, 538, 745
NlaIV GGNNCC 3 cut(s) 88, 240, 560
NmuCI GTSAC 1 cut(s) 32
PasI CCCWGGG 1 cut(s) 242
PctI GAATGC 1 cut(s) 163
PfeI GAWTC 2 cut(s) 112, 233
PkrI GCNGC 2 cut(s) 364, 541
PleI GAGTC 1 cut(s) 499
PpsI GAGTC 1 cut(s) 499
Ppu21I YACGTR 1 cut(s) 374
Psp6I CCWGG 2 cut(s) 241, 754
PspFI CCCAGC 1 cut(s) 203
PspGI CCWGG 2 cut(s) 241, 754
PspN4I GGNNCC 3 cut(s) 88, 240, 560
PspPI GGNCC 4 cut(s) 87, 238, 310, 568
PsuI RGATCY 1 cut(s) 799
RsaI GTAC 3 cut(s) 136, 296, 350
RsaNI GTAC 3 cut(s) 135, 295, 349
SaqAI TTAA 3 cut(s) 177, 599, 735
SatI GCNGC 2 cut(s) 363, 540
Sau3AI GATC 2 cut(s) 357, 799
Sau96I GGNCC 4 cut(s) 87, 238, 310, 568
SchI GAGTC 1 cut(s) 500
ScrFI CCNGG 3 cut(s) 85, 243, 756
SduI GDGCHC 2 cut(s) 249, 561
SfaNI GCATC 1 cut(s) 625
SinI GGWCC 2 cut(s) 238, 568
SmlI CTYRAG 2 cut(s) 248, 636
SmoI CTYRAG 2 cut(s) 248, 636
Sse9I AATT 4 cut(s) 124, 192, 299, 651
SsiI CCGC 2 cut(s) 362, 706
SspMI CTAG 1 cut(s) 420
StyD4I CCNGG 3 cut(s) 83, 241, 754
StyI CCWWGG 1 cut(s) 313
TaaI ACNGT 4 cut(s) 32, 53, 294, 385
TaiI ACGT 1 cut(s) 376
TaqI TCGA 2 cut(s) 14, 231
TaqII GACCGA 1 cut(s) 226
TasI AATT 4 cut(s) 124, 192, 299, 651
TatI WGTACW 1 cut(s) 294
TauI GCSGC 1 cut(s) 365
TfiI GAWTC 2 cut(s) 112, 233
Tru1I TTAA 3 cut(s) 177, 599, 735
Tru9I TTAA 3 cut(s) 177, 599, 735
TscAI CASTG 2 cut(s) 534, 786
TseFI GTSAC 1 cut(s) 32
TseI GCWGC 1 cut(s) 539
Tsp45I GTSAC 1 cut(s) 32
TspDTI ATGAA 4 cut(s) 201, 345, 606, 660
TspGWI ACGGA 1 cut(s) 152
TspRI CASTG 2 cut(s) 534, 786
VpaK11BI GGWCC 2 cut(s) 238, 568
XcmI CCANNNNNNNNNTGG 1 cut(s) 619
XspI CTAG 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.