RLG00000005678

Protein of unknown function (DUF3755)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0004772
Physical Location & Seq
Reverse (-)
773107 .. 777218
4112 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005678

Sequence Viewer

Length: 1032 bp
ATGGCGATGGAGTCCAACACCGGGTTTCTACATGATGAGACTTTTGGCTATGGCTTGAACCGGCACGCGATATCTTTCCAGTCTGGAGCAATAAACAGCAGCTCGGAGATGATTCCGATGGGGAATTACTTTAGTACTGACCCGGTGATGGGGATGGGGATGGGGATGATGTTCTCTCCCAATTCGGGGTCTGGCATGGGCATGGGCATGGGCATGGTCAACCACAGTCCTGTAATCAGTCAACCCGGGACTTCATCAGGGGCTTCTCTGCTTGTTGATTCGGTACCGGGCCTCAAGCATGACACAGGCTTGGCAGTTGAGTGGTCTATGGAGGAGCAATACAAATTGGAGGAGGGCCTTGTCAAATTTGCTGATGAACCAAGTACTATGAGATATGCTGATGAACCGAGTATTATGAGGTACATAAAGATTGCGGCAACCTTGCGTGATAAAACTGTGCGTGATGTTGCCTTGAGGTGTAGGTGGATGACGAGAAAGCGGAGGAAACCTGAAGACCATACTACAGGAAAGAAGGGCAACATTAGGAAGGATAAAGTGGTAGATTCATACTCCAAGATGAGCATTCCCTCAGCTCCACAACACAACATGGCTGCATATTCTCTTATGATGCATCGTATGAACCAGAATGAGCGTTTGCAGTGTGAAGGAATAAGTGGGACGGCCAAGCATTTATTGGACCAAAATGCTCAAGCTTTTAATCAAATCACAGCTAACCTTTCTACGTACAAGTTACAGGACAATATTAACCTCTTCTGTCGCACAAGGAACAACTTAACTGCCATCTTGAATGACATGAGAGTGTTATCTTGCCTAATACAACTTAGACAAATCAACATCTCGAAATCCTGGACTGCCTCATTGTCTGAGCGCACTGTGTATGAGTTTGCTTTTGTTGCCAGCAAGCAGAGTTGCAGATATCTGATGAGGCTTATGCAGAACCCTACTCAATTCTTGGCCTCACTGTCATTTGATCTTAGTGCCTCCAGGCCTGGATCCTTGTGTTGTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3755

Protein Analysis

344

Amino Acids

38.29

Weight (kDa)

9.01

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3755 PF12579 248 - 273 4.2e-12 Protein of unknown function (DUF3755)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 283
AccB1I GGYRCC 1 cut(s) 283
AccII CGCG 1 cut(s) 68
AciI CCGC 2 cut(s) 434, 499
AclWI GGATC 2 cut(s) 1008, 1021
AcoI YGGCCR 1 cut(s) 681
AcsI RAATTY 1 cut(s) 365
AcuI CTGAAG 1 cut(s) 531
AfaI GTAC 5 cut(s) 136, 285, 385, 422, 746
AfiI CCNNNNNNNGG 5 cut(s) 21, 148, 149, 185, 186
AgsI TTSAA 2 cut(s) 58, 808
AjnI CCWGG 3 cut(s) 866, 1004, 1009
AluBI AGCT 4 cut(s) 102, 593, 713, 731
AluI AGCT 4 cut(s) 102, 593, 713, 731
Alw26I GTCTC 1 cut(s) 32
AlwI GGATC 2 cut(s) 1008, 1021
Ama87I CYCGRG 1 cut(s) 245
AoxI GGCC 5 cut(s) 289, 355, 681, 975, 1007
ApeKI GCWGC 2 cut(s) 99, 611
ApoI RAATTY 1 cut(s) 365
Asp718I GGTACC 1 cut(s) 283
AspLEI GCGC 1 cut(s) 891
AspS9I GGNCC 3 cut(s) 289, 355, 697
AsuC2I CCSGG 5 cut(s) 22, 143, 246, 247, 288
AsuHPI GGTGA 1 cut(s) 157
AvaI CYCGRG 1 cut(s) 245
AvaII GGWCC 1 cut(s) 697
BamHI GGATCC 1 cut(s) 1013
BanI GGYRCC 1 cut(s) 283
BbsI GAAGAC 1 cut(s) 519
BbvCI CCTCAGC 1 cut(s) 589
BbvI GCAGC 2 cut(s) 111, 598
BccI CCATC 5 cut(s) 112, 142, 148, 154, 809
BceAI ACGGC 1 cut(s) 696
BciT130I CCWGG 3 cut(s) 868, 1006, 1011
BcnI CCSGG 5 cut(s) 22, 143, 246, 247, 288
BcoDI GTCTC 1 cut(s) 32
BfmI CTRYAG 1 cut(s) 522
BisI GCNGC 3 cut(s) 100, 435, 612
BlsI GCNGC 3 cut(s) 101, 436, 613
BmcAI AGTACT 2 cut(s) 136, 385
Bme1390I CCNGG 8 cut(s) 22, 143, 246, 247, 288, 868, 1006, 1011
Bme18I GGWCC 1 cut(s) 697
BmeT110I CYCGRG 1 cut(s) 245
BmgT120I GGNCC 3 cut(s) 289, 355, 697
BmiI GGNNCC 2 cut(s) 285, 1015
BmrFI CCNGG 8 cut(s) 22, 143, 246, 247, 288, 868, 1006, 1011
BmsI GCATC 2 cut(s) 618, 640
BpiI GAAGAC 1 cut(s) 519
BpmI CTGGAG 2 cut(s) 105, 988
Bpu10I CCTNAGC 1 cut(s) 589
BpuEI CTTGAG 3 cut(s) 278, 493, 693
BpuMI CCSGG 5 cut(s) 22, 143, 246, 247, 288
BsaAI YACGTR 1 cut(s) 744
BsaJI CCNNGG 1 cut(s) 245
Bsc4I CCNNNNNNNGG 5 cut(s) 21, 148, 149, 185, 186
Bse118I RCCGGY 1 cut(s) 60
Bse1I ACTGG 1 cut(s) 79
BseBI CCWGG 3 cut(s) 868, 1006, 1011
BseDI CCNNGG 1 cut(s) 245
BseGI GGATG 4 cut(s) 159, 165, 171, 492
BseLI CCNNNNNNNGG 5 cut(s) 21, 148, 149, 185, 186
BseMII CTCAG 2 cut(s) 603, 876
BseNI ACTGG 1 cut(s) 79
BseRI GAGGAG 2 cut(s) 347, 365
BseXI GCAGC 2 cut(s) 111, 598
Bsh1236I CGCG 1 cut(s) 68
BshFI GGCC 5 cut(s) 291, 357, 683, 977, 1009
BshNI GGYRCC 1 cut(s) 283
BsiHKCI CYCGRG 1 cut(s) 245
BsiSI CCGG 5 cut(s) 21, 61, 143, 246, 287
BslFI GGGAC 2 cut(s) 262, 691
BslI CCNNNNNNNGG 5 cut(s) 21, 148, 149, 185, 186
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 2 cut(s) 262, 691
BsmI GAATGC 1 cut(s) 582
BsnI GGCC 5 cut(s) 291, 357, 683, 977, 1009
BsoBI CYCGRG 1 cut(s) 245
Bsp143I GATC 2 cut(s) 991, 1013
BspACI CCGC 2 cut(s) 434, 499
BspANI GGCC 5 cut(s) 291, 357, 683, 977, 1009
BspCNI CTCAG 2 cut(s) 602, 877
BspFNI CGCG 1 cut(s) 68
BspLI GGNNCC 2 cut(s) 285, 1015
BspPI GGATC 2 cut(s) 1008, 1021
BspT107I GGYRCC 1 cut(s) 283
BsrFI RCCGGY 1 cut(s) 60
BsrI ACTGG 1 cut(s) 79
BssAI RCCGGY 1 cut(s) 60
BssECI CCNNGG 1 cut(s) 245
BssMI GATC 2 cut(s) 991, 1013
Bst2UI CCWGG 3 cut(s) 868, 1006, 1011
Bst4CI ACNGT 4 cut(s) 227, 457, 895, 984
Bst6I CTCTTC 1 cut(s) 776
BstBAI YACGTR 1 cut(s) 744
BstC8I GCNNGC 3 cut(s) 66, 919, 923
BstDEI CTNAG 4 cut(s) 589, 842, 885, 995
BstF5I GGATG 4 cut(s) 159, 165, 171, 492
BstFNI CGCG 1 cut(s) 68
BstHHI GCGC 1 cut(s) 891
BstKTI GATC 2 cut(s) 994, 1016
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 2 cut(s) 991, 1013
BstMWI GCNNNNNNNGC 1 cut(s) 914
BstNI CCWGG 3 cut(s) 868, 1006, 1011
BstSCI CCNGG 8 cut(s) 20, 141, 244, 245, 286, 866, 1004, 1009
BstSFI CTRYAG 1 cut(s) 522
BstSNI TACGTA 1 cut(s) 744
BstUI CGCG 1 cut(s) 68
BstV1I GCAGC 2 cut(s) 111, 598
BstV2I GAAGAC 1 cut(s) 519
BstX2I RGATCY 1 cut(s) 1013
BstYI RGATCY 1 cut(s) 1013
BsuRI GGCC 5 cut(s) 291, 357, 683, 977, 1009
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 4 cut(s) 159, 165, 171, 492
BtsI GCAGTG 1 cut(s) 665
BtsIMutI CAGTG 3 cut(s) 665, 891, 980
Cac8I GCNNGC 3 cut(s) 66, 919, 923
CfoI GCGC 1 cut(s) 891
Cfr10I RCCGGY 1 cut(s) 60
Cfr13I GGNCC 3 cut(s) 289, 355, 697
Cfr9I CCCGGG 1 cut(s) 245
Csp6I GTAC 5 cut(s) 135, 284, 384, 421, 745
CviAII CATG 8 cut(s) 32, 196, 202, 208, 214, 299, 607, 814
CviQI GTAC 5 cut(s) 135, 284, 384, 421, 745
DdeI CTNAG 4 cut(s) 589, 842, 885, 995
DpnI GATC 2 cut(s) 993, 1015
DpnII GATC 2 cut(s) 991, 1013
EaeI YGGCCR 1 cut(s) 681
Eam1104I CTCTTC 1 cut(s) 776
EarI CTCTTC 1 cut(s) 776
Eco105I TACGTA 1 cut(s) 744
Eco147I AGGCCT 1 cut(s) 1009
Eco32I GATATC 2 cut(s) 72, 938
Eco47I GGWCC 1 cut(s) 697
Eco57I CTGAAG 1 cut(s) 531
Eco88I CYCGRG 1 cut(s) 245
EcoO109I RGGNCCY 1 cut(s) 355
EcoRII CCWGG 3 cut(s) 866, 1004, 1009
EcoRV GATATC 2 cut(s) 72, 938
EcoT22I ATGCAT 1 cut(s) 633
FaeI CATG 8 cut(s) 35, 199, 205, 211, 217, 302, 610, 817
FaqI GGGAC 2 cut(s) 262, 691
FatI CATG 8 cut(s) 31, 195, 201, 207, 213, 298, 606, 813
Fnu4HI GCNGC 3 cut(s) 100, 435, 612
FokI GGATG 4 cut(s) 166, 172, 178, 499
Fsp4HI GCNGC 3 cut(s) 100, 435, 612
GlaI GCGC 1 cut(s) 890
GluI GCNGC 3 cut(s) 100, 435, 612
GsuI CTGGAG 2 cut(s) 105, 988
HaeIII GGCC 5 cut(s) 291, 357, 683, 977, 1009
HapII CCGG 5 cut(s) 21, 61, 143, 246, 287
HhaI GCGC 1 cut(s) 891
Hin1II CATG 8 cut(s) 35, 199, 205, 211, 217, 302, 610, 817
Hin6I GCGC 1 cut(s) 889
HinP1I GCGC 1 cut(s) 889
HincII GTYRAC 2 cut(s) 220, 242
HindII GTYRAC 2 cut(s) 220, 242
HindIII AAGCTT 1 cut(s) 711
HinfI GANTC 4 cut(s) 11, 112, 278, 563
HpaII CCGG 5 cut(s) 21, 61, 143, 246, 287
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 2 cut(s) 220, 242
Hpy188I TCNGA 4 cut(s) 106, 117, 886, 942
Hpy188III TCNNGA 3 cut(s) 84, 805, 859
Hpy8I GTNNAC 2 cut(s) 220, 242
HpyAV CCTTC 3 cut(s) 526, 541, 659
HpyCH4III ACNGT 4 cut(s) 227, 457, 895, 984
HpyCH4IV ACGT 1 cut(s) 743
HpyCH4V TGCA 5 cut(s) 614, 631, 658, 933, 955
HpyF10VI GCNNNNNNNGC 1 cut(s) 914
HpyF3I CTNAG 4 cut(s) 589, 842, 885, 995
HpySE526I ACGT 1 cut(s) 743
Hsp92II CATG 8 cut(s) 35, 199, 205, 211, 217, 302, 610, 817
HspAI GCGC 1 cut(s) 889
KpnI GGTACC 1 cut(s) 287
Kzo9I GATC 2 cut(s) 991, 1013
LmnI GCTCC 3 cut(s) 86, 334, 598
Lsp1109I GCAGC 2 cut(s) 111, 598
LweI GCATC 2 cut(s) 618, 640
MaeII ACGT 1 cut(s) 743
MaeIII GTNAC 1 cut(s) 750
MalI GATC 2 cut(s) 993, 1015
MboI GATC 2 cut(s) 991, 1013
MboII GAAGA 2 cut(s) 524, 763
MflI RGATCY 1 cut(s) 1013
MluCI AATT 5 cut(s) 124, 181, 344, 365, 968
MlyI GAGTC 1 cut(s) 20
MmeI TCCRAC 1 cut(s) 39
Mph1103I ATGCAT 1 cut(s) 633
MseI TTAA 3 cut(s) 717, 765, 794
MslI CAYNNNNRTG 4 cut(s) 200, 206, 212, 818
MspI CCGG 5 cut(s) 21, 61, 143, 246, 287
MspR9I CCNGG 8 cut(s) 22, 143, 246, 247, 288, 868, 1006, 1011
Mva1269I GAATGC 1 cut(s) 582
MvaI CCWGG 3 cut(s) 868, 1006, 1011
MvnI CGCG 1 cut(s) 68
MwoI GCNNNNNNNGC 1 cut(s) 914
NciI CCSGG 5 cut(s) 22, 143, 246, 247, 288
NdeII GATC 2 cut(s) 991, 1013
NlaIII CATG 8 cut(s) 35, 199, 205, 211, 217, 302, 610, 817
NlaIV GGNNCC 2 cut(s) 285, 1015
NsiI ATGCAT 1 cut(s) 633
PceI AGGCCT 1 cut(s) 1009
PctI GAATGC 1 cut(s) 582
PfeI GAWTC 3 cut(s) 112, 278, 563
PfoI TCCNGGA 1 cut(s) 866
PkrI GCNGC 3 cut(s) 101, 436, 613
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
Ppu21I YACGTR 1 cut(s) 744
Psp6I CCWGG 3 cut(s) 866, 1004, 1009
PspGI CCWGG 3 cut(s) 866, 1004, 1009
PspN4I GGNNCC 2 cut(s) 285, 1015
PspPI GGNCC 3 cut(s) 289, 355, 697
PsuI RGATCY 1 cut(s) 1013
RsaI GTAC 5 cut(s) 136, 285, 385, 422, 746
RsaNI GTAC 5 cut(s) 135, 284, 384, 421, 745
RseI CAYNNNNRTG 4 cut(s) 200, 206, 212, 818
SaqAI TTAA 3 cut(s) 717, 765, 794
SatI GCNGC 3 cut(s) 100, 435, 612
Sau3AI GATC 2 cut(s) 991, 1013
Sau96I GGNCC 3 cut(s) 289, 355, 697
ScaI AGTACT 2 cut(s) 136, 385
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 8 cut(s) 22, 143, 246, 247, 288, 868, 1006, 1011
SfaNI GCATC 2 cut(s) 618, 640
SfcI CTRYAG 1 cut(s) 522
SinI GGWCC 1 cut(s) 697
SmaI CCCGGG 1 cut(s) 247
SmiMI CAYNNNNRTG 4 cut(s) 200, 206, 212, 818
SmlI CTYRAG 3 cut(s) 293, 472, 708
SmoI CTYRAG 3 cut(s) 293, 472, 708
SnaBI TACGTA 1 cut(s) 744
Sse9I AATT 5 cut(s) 124, 181, 344, 365, 968
SseBI AGGCCT 1 cut(s) 1009
SsiI CCGC 2 cut(s) 434, 499
SspI AATATT 1 cut(s) 763
StuI AGGCCT 1 cut(s) 1009
StyD4I CCNGG 8 cut(s) 20, 141, 244, 245, 286, 866, 1004, 1009
TaaI ACNGT 4 cut(s) 227, 457, 895, 984
TaiI ACGT 1 cut(s) 746
TaqI TCGA 1 cut(s) 860
TasI AATT 5 cut(s) 124, 181, 344, 365, 968
TatI WGTACW 2 cut(s) 134, 383
TauI GCSGC 1 cut(s) 437
TfiI GAWTC 3 cut(s) 112, 278, 563
Tru1I TTAA 3 cut(s) 717, 765, 794
Tru9I TTAA 3 cut(s) 717, 765, 794
TscAI CASTG 3 cut(s) 665, 898, 987
TseI GCWGC 2 cut(s) 99, 611
TspDTI ATGAA 5 cut(s) 243, 390, 417, 555, 653
TspMI CCCGGG 1 cut(s) 245
TspRI CASTG 3 cut(s) 665, 898, 987
VpaK11BI GGWCC 1 cut(s) 697
XapI RAATTY 1 cut(s) 365
XcmI CCANNNNNNNNNTGG 1 cut(s) 691
XmaI CCCGGG 1 cut(s) 245
ZrmI AGTACT 2 cut(s) 136, 385
Zsp2I ATGCAT 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.