Rh4CG457600

Protein of unknown function (DUF3755)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0004772
Physical Location & Seq
Forward (+)
68928412 .. 68932417
4006 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG457600.1

Sequence Viewer

Length: 900 bp
ATGGCGATGGAGTCGAACACCGGGTTTCTACATGATGAGACTTTTGGCTATGGCTTGAACCGGCACGCGATATCTTTCCAGTCTGGAGCAATAAACAGCAGCTCGGAGATGATTCCGATGGGGAATTACTTTAGTACTGACCCGGTGATGGGGATGGGGATGATGTTCTCTCCGAATTTGGGGTCAGGCATGGGCATGGGCATGGTCAACCACAGTCCTGTAATTAGTCAACCCGGGACTTCATCAGGGGCTTCTCTGCTTGTTGATTCGGTACCGGGCCTCAAGCATGACACAGGCTTGGCAGTTGAGTGGTCTATGGAGGAGCAATACAAATTGGAGGAGGGCCTTGTCAAATTTGCTGATGAACCAAGTACTATGAGGTATGCTGATGAACCGAGTATTATGAGGTACATAAAGATTGCGGCAACCTTGCGTGATAAAACTGTGCGTGATGTTGCCTTGAGGTGTAGGTGGATGACGAGAAAGCGAAGGAAACCTGAAGACCATACTACAGGAAAGAAGGGCAACATTAGGAAGGATAAACTGGTAGAATCATACTCCAAGATGAGCATACCCTCAGCTCCACAACACAACATGGCTGCATATTCTCTTATGATGCATCGTATGAACCAGAATGAGCGTTTGCAGTGTGAAGGAATAAGTGGGACAGCCAAGCATTTATTGGACCAAAATGCTCAAGCTTTTAATCAAATCACAGCTAACCTTTCTACGTACAAGTTACAGGACAATATTAACCTCTTCTGTCGCACAAGGAACAACTTAACTGCCATCTTGAATGACATGAGAGGTATGCCTGGCTTAATGAGCCAAATGCCACCGCTGGATGTATGCATAAATGAGGACCTTGCTAATAGTGTTATCTTGCGTAATACAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3755

Protein Analysis

299

Amino Acids

33.3

Weight (kDa)

7.72

Isoelectric Point (pI)

47.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3755 PF12579 244 - 277 7.5e-19 Protein of unknown function (DUF3755)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 271
AccB1I GGYRCC 1 cut(s) 271
AccII CGCG 1 cut(s) 68
AciI CCGC 2 cut(s) 422, 839
AcsI RAATTY 2 cut(s) 175, 353
AcuI CTGAAG 1 cut(s) 519
AfaI GTAC 5 cut(s) 136, 273, 373, 410, 734
AfiI CCNNNNNNNGG 3 cut(s) 148, 149, 179
AgsI TTSAA 2 cut(s) 58, 796
AjnI CCWGG 1 cut(s) 814
AluBI AGCT 4 cut(s) 102, 581, 701, 719
AluI AGCT 4 cut(s) 102, 581, 701, 719
Alw26I GTCTC 1 cut(s) 32
Ama87I CYCGRG 1 cut(s) 233
AoxI GGCC 2 cut(s) 277, 343
ApeKI GCWGC 2 cut(s) 99, 599
ApoI RAATTY 2 cut(s) 175, 353
ArsI GACNNNNNNTTYG 2 cut(s) 167, 199
Asp718I GGTACC 1 cut(s) 271
AspS9I GGNCC 4 cut(s) 277, 343, 685, 862
AsuC2I CCSGG 5 cut(s) 22, 143, 234, 235, 276
AsuHPI GGTGA 1 cut(s) 157
AvaI CYCGRG 1 cut(s) 233
AvaII GGWCC 2 cut(s) 685, 862
BanI GGYRCC 1 cut(s) 271
BbsI GAAGAC 1 cut(s) 507
BbvCI CCTCAGC 1 cut(s) 577
BbvI GCAGC 2 cut(s) 111, 586
BccI CCATC 4 cut(s) 112, 142, 148, 797
BciT130I CCWGG 1 cut(s) 816
BcnI CCSGG 5 cut(s) 22, 143, 234, 235, 276
BcoDI GTCTC 1 cut(s) 32
BfmI CTRYAG 1 cut(s) 510
BisI GCNGC 3 cut(s) 100, 423, 600
BlsI GCNGC 3 cut(s) 101, 424, 601
BmcAI AGTACT 2 cut(s) 136, 373
Bme1390I CCNGG 6 cut(s) 22, 143, 234, 235, 276, 816
Bme18I GGWCC 2 cut(s) 685, 862
BmeT110I CYCGRG 1 cut(s) 233
BmgT120I GGNCC 4 cut(s) 277, 343, 685, 862
BmiI GGNNCC 1 cut(s) 273
BmrFI CCNGG 6 cut(s) 22, 143, 234, 235, 276, 816
BmsI GCATC 2 cut(s) 606, 628
BpiI GAAGAC 1 cut(s) 507
BpmI CTGGAG 1 cut(s) 105
Bpu10I CCTNAGC 1 cut(s) 577
BpuEI CTTGAG 3 cut(s) 266, 481, 681
BpuMI CCSGG 5 cut(s) 22, 143, 234, 235, 276
BsaAI YACGTR 1 cut(s) 732
BsaJI CCNNGG 1 cut(s) 233
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 149, 179
Bse118I RCCGGY 1 cut(s) 60
Bse1I ACTGG 2 cut(s) 79, 549
BseBI CCWGG 1 cut(s) 816
BseDI CCNNGG 1 cut(s) 233
BseGI GGATG 4 cut(s) 159, 165, 480, 850
BseLI CCNNNNNNNGG 3 cut(s) 148, 149, 179
BseMII CTCAG 1 cut(s) 591
BseNI ACTGG 2 cut(s) 79, 549
BseRI GAGGAG 2 cut(s) 335, 353
BseXI GCAGC 2 cut(s) 111, 586
Bsh1236I CGCG 1 cut(s) 68
BshFI GGCC 2 cut(s) 279, 345
BshNI GGYRCC 1 cut(s) 271
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 5 cut(s) 21, 61, 143, 234, 275
BslFI GGGAC 2 cut(s) 250, 679
BslI CCNNNNNNNGG 3 cut(s) 148, 149, 179
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 2 cut(s) 250, 679
BsnI GGCC 2 cut(s) 279, 345
BsoBI CYCGRG 1 cut(s) 233
BspACI CCGC 2 cut(s) 422, 839
BspANI GGCC 2 cut(s) 279, 345
BspCNI CTCAG 1 cut(s) 590
BspFNI CGCG 1 cut(s) 68
BspLI GGNNCC 1 cut(s) 273
BspT107I GGYRCC 1 cut(s) 271
BsrFI RCCGGY 1 cut(s) 60
BsrI ACTGG 2 cut(s) 79, 549
BssAI RCCGGY 1 cut(s) 60
BssECI CCNNGG 1 cut(s) 233
Bst2UI CCWGG 1 cut(s) 816
Bst4CI ACNGT 2 cut(s) 215, 445
Bst6I CTCTTC 1 cut(s) 764
BstBAI YACGTR 1 cut(s) 732
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 2 cut(s) 577, 897
BstF5I GGATG 4 cut(s) 159, 165, 480, 850
BstFNI CGCG 1 cut(s) 68
BstMAI GTCTC 1 cut(s) 32
BstMWI GCNNNNNNNGC 1 cut(s) 825
BstNI CCWGG 1 cut(s) 816
BstSCI CCNGG 6 cut(s) 20, 141, 232, 233, 274, 814
BstSFI CTRYAG 1 cut(s) 510
BstSNI TACGTA 1 cut(s) 732
BstUI CGCG 1 cut(s) 68
BstV1I GCAGC 2 cut(s) 111, 586
BstV2I GAAGAC 1 cut(s) 507
BsuRI GGCC 2 cut(s) 279, 345
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 4 cut(s) 159, 165, 480, 850
BtsI GCAGTG 1 cut(s) 653
BtsIMutI CAGTG 1 cut(s) 653
Cac8I GCNNGC 1 cut(s) 66
Cfr10I RCCGGY 1 cut(s) 60
Cfr13I GGNCC 4 cut(s) 277, 343, 685, 862
Cfr9I CCCGGG 1 cut(s) 233
Csp6I GTAC 5 cut(s) 135, 272, 372, 409, 733
CviAII CATG 7 cut(s) 32, 190, 196, 202, 287, 595, 802
CviQI GTAC 5 cut(s) 135, 272, 372, 409, 733
DdeI CTNAG 2 cut(s) 577, 897
Eam1104I CTCTTC 1 cut(s) 764
EarI CTCTTC 1 cut(s) 764
Eco105I TACGTA 1 cut(s) 732
Eco32I GATATC 1 cut(s) 72
Eco47I GGWCC 2 cut(s) 685, 862
Eco57I CTGAAG 1 cut(s) 519
Eco88I CYCGRG 1 cut(s) 233
EcoO109I RGGNCCY 2 cut(s) 343, 862
EcoRII CCWGG 1 cut(s) 814
EcoRV GATATC 1 cut(s) 72
EcoT22I ATGCAT 2 cut(s) 621, 854
FaeI CATG 7 cut(s) 35, 193, 199, 205, 290, 598, 805
FaqI GGGAC 2 cut(s) 250, 679
FatI CATG 7 cut(s) 31, 189, 195, 201, 286, 594, 801
Fnu4HI GCNGC 3 cut(s) 100, 423, 600
FokI GGATG 4 cut(s) 166, 172, 487, 857
Fsp4HI GCNGC 3 cut(s) 100, 423, 600
GluI GCNGC 3 cut(s) 100, 423, 600
GsuI CTGGAG 1 cut(s) 105
HaeIII GGCC 2 cut(s) 279, 345
HapII CCGG 5 cut(s) 21, 61, 143, 234, 275
Hin1II CATG 7 cut(s) 35, 193, 199, 205, 290, 598, 805
HincII GTYRAC 2 cut(s) 208, 230
HindII GTYRAC 2 cut(s) 208, 230
HindIII AAGCTT 1 cut(s) 699
HinfI GANTC 4 cut(s) 11, 112, 266, 551
HpaII CCGG 5 cut(s) 21, 61, 143, 234, 275
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 2 cut(s) 208, 230
Hpy188I TCNGA 3 cut(s) 106, 117, 174
Hpy188III TCNNGA 2 cut(s) 84, 793
Hpy8I GTNNAC 2 cut(s) 208, 230
HpyAV CCTTC 4 cut(s) 483, 514, 529, 647
HpyCH4III ACNGT 2 cut(s) 215, 445
HpyCH4IV ACGT 1 cut(s) 731
HpyCH4V TGCA 4 cut(s) 602, 619, 646, 852
HpyF10VI GCNNNNNNNGC 1 cut(s) 825
HpyF3I CTNAG 2 cut(s) 577, 897
HpySE526I ACGT 1 cut(s) 731
Hsp92II CATG 7 cut(s) 35, 193, 199, 205, 290, 598, 805
KpnI GGTACC 1 cut(s) 275
LmnI GCTCC 3 cut(s) 86, 322, 586
Lsp1109I GCAGC 2 cut(s) 111, 586
LweI GCATC 2 cut(s) 606, 628
MaeII ACGT 1 cut(s) 731
MaeIII GTNAC 1 cut(s) 738
MboII GAAGA 2 cut(s) 512, 751
MluCI AATT 5 cut(s) 124, 175, 222, 332, 353
MlyI GAGTC 1 cut(s) 20
Mph1103I ATGCAT 2 cut(s) 621, 854
MseI TTAA 4 cut(s) 705, 753, 782, 821
MslI CAYNNNNRTG 2 cut(s) 194, 200
MspA1I CMGCKG 1 cut(s) 841
MspI CCGG 5 cut(s) 21, 61, 143, 234, 275
MspR9I CCNGG 6 cut(s) 22, 143, 234, 235, 276, 816
MvaI CCWGG 1 cut(s) 816
MvnI CGCG 1 cut(s) 68
MwoI GCNNNNNNNGC 1 cut(s) 825
NciI CCSGG 5 cut(s) 22, 143, 234, 235, 276
NlaIII CATG 7 cut(s) 35, 193, 199, 205, 290, 598, 805
NlaIV GGNNCC 1 cut(s) 273
NsiI ATGCAT 2 cut(s) 621, 854
PfeI GAWTC 3 cut(s) 112, 266, 551
PkrI GCNGC 3 cut(s) 101, 424, 601
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
Ppu21I YACGTR 1 cut(s) 732
PpuMI RGGWCCY 1 cut(s) 862
Psp5II RGGWCCY 1 cut(s) 862
Psp6I CCWGG 1 cut(s) 814
PspGI CCWGG 1 cut(s) 814
PspN4I GGNNCC 1 cut(s) 273
PspPI GGNCC 4 cut(s) 277, 343, 685, 862
PspPPI RGGWCCY 1 cut(s) 862
RsaI GTAC 5 cut(s) 136, 273, 373, 410, 734
RsaNI GTAC 5 cut(s) 135, 272, 372, 409, 733
RseI CAYNNNNRTG 2 cut(s) 194, 200
SaqAI TTAA 4 cut(s) 705, 753, 782, 821
SatI GCNGC 3 cut(s) 100, 423, 600
Sau96I GGNCC 4 cut(s) 277, 343, 685, 862
ScaI AGTACT 2 cut(s) 136, 373
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 6 cut(s) 22, 143, 234, 235, 276, 816
SfaNI GCATC 2 cut(s) 606, 628
SfcI CTRYAG 1 cut(s) 510
SinI GGWCC 2 cut(s) 685, 862
SmaI CCCGGG 1 cut(s) 235
SmiMI CAYNNNNRTG 2 cut(s) 194, 200
SmlI CTYRAG 3 cut(s) 281, 460, 696
SmoI CTYRAG 3 cut(s) 281, 460, 696
SnaBI TACGTA 1 cut(s) 732
Sse9I AATT 5 cut(s) 124, 175, 222, 332, 353
SsiI CCGC 2 cut(s) 422, 839
SspI AATATT 1 cut(s) 751
StyD4I CCNGG 6 cut(s) 20, 141, 232, 233, 274, 814
TaaI ACNGT 2 cut(s) 215, 445
TaiI ACGT 1 cut(s) 734
TaqI TCGA 1 cut(s) 14
TasI AATT 5 cut(s) 124, 175, 222, 332, 353
TatI WGTACW 2 cut(s) 134, 371
TauI GCSGC 1 cut(s) 425
TfiI GAWTC 3 cut(s) 112, 266, 551
Tru1I TTAA 4 cut(s) 705, 753, 782, 821
Tru9I TTAA 4 cut(s) 705, 753, 782, 821
TscAI CASTG 1 cut(s) 653
TseI GCWGC 2 cut(s) 99, 599
TspDTI ATGAA 4 cut(s) 231, 378, 405, 641
TspMI CCCGGG 1 cut(s) 233
TspRI CASTG 1 cut(s) 653
VpaK11BI GGWCC 2 cut(s) 685, 862
XapI RAATTY 2 cut(s) 175, 353
XcmI CCANNNNNNNNNTGG 1 cut(s) 679
XmaI CCCGGG 1 cut(s) 233
ZrmI AGTACT 2 cut(s) 136, 373
Zsp2I ATGCAT 2 cut(s) 621, 854
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.