Rh4DG438800

Protein of unknown function (DUF3755)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004772
Physical Location & Seq
Forward (+)
64139753 .. 64144810
5058 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG438800.1

Sequence Viewer

Length: 912 bp
ATGGCGATGGAGTCGAACACCGGGTTTCTACATGATGACACTTTTGGCTATGGCTTGAACCGGCACGCTATATCTTTCCAGTCTGGAGCAATAAACAGCAGCTCGGAGATGATTCCGATGGGGAATTACTTTAGTACTGACCCGGTGATGGGGATGGGGATGGGGATGCAGATGATGTTCTCTCCGACTTCGGGGTCAGGCATGGGCATGGGCATGGTCAACCACAGTCCTGCAATTAGTCAACCCGGGACTTCATCAGGGTCTTCTCTGCTCGTTGATTCCGTACCGGGCCTCAAGCATGACACAGGCTTGGCAGTTGAGTGGTCTTTGGAGGAGCAATACAAATTGGAGGAGGGCCTTGTCAAATTTGCTGATGAACCAAGTACTATGAGATATGCTGATGAACCGAGTATTATGAGGTACATAAAGATTGCGGCAACCTTGCGTGATAAAACTGTGCGTGATGTTGCCTTGAGGTGTAGGTGGATGACGAGAAAGCGGAGGAAACCTGAAGACCATACTACAGGAAAGAAGGGCAACATTAGGAAGGATAAACTGGTAGATTCATACTCCAAGATGAGCATACCCTCAGCTCCACAACACAACATGGCTGCATATTCTCTTATGATGCATCGTATGAACCAGAATGAGCGTTTGCAGTGTGAAGGAATAAGTGGGACAGCCAAGCATTTATTGGACCAAAATGCTCAAGCTTTTAATCAAATCACAGCTAACCTTTCTACGTACAAGTTACAGGACAATATTAACCTCTTCTGTCGCACAAGGAACAACTTAACTGCCATCTTGAATGACATGAGAGGTATGCCTGGCTTAATGAGCCAAATGCCGCCACTGGATGTATGCATAAATGAGAACCTTGCTAATAGTGTTATCTTGCCTAATACAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3755

Protein Analysis

303

Amino Acids

33.59

Weight (kDa)

7.72

Isoelectric Point (pI)

45.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3755 PF12579 248 - 281 7.7e-19 Protein of unknown function (DUF3755)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 193
AciI CCGC 3 cut(s) 434, 499, 848
AcsI RAATTY 1 cut(s) 365
AcuI CTGAAG 1 cut(s) 531
AfaI GTAC 5 cut(s) 136, 285, 385, 422, 746
AfiI CCNNNNNNNGG 3 cut(s) 148, 149, 191
AgsI TTSAA 2 cut(s) 58, 808
AjnI CCWGG 1 cut(s) 826
AluBI AGCT 4 cut(s) 102, 593, 713, 731
AluI AGCT 4 cut(s) 102, 593, 713, 731
Ama87I CYCGRG 1 cut(s) 245
AoxI GGCC 2 cut(s) 289, 355
ApeKI GCWGC 2 cut(s) 99, 611
ApoI RAATTY 1 cut(s) 365
AspS9I GGNCC 3 cut(s) 289, 355, 697
AsuC2I CCSGG 5 cut(s) 22, 143, 246, 247, 288
AsuHPI GGTGA 1 cut(s) 157
AvaI CYCGRG 1 cut(s) 245
AvaII GGWCC 1 cut(s) 697
BbsI GAAGAC 2 cut(s) 255, 519
BbvCI CCTCAGC 1 cut(s) 589
BbvI GCAGC 2 cut(s) 111, 598
BccI CCATC 5 cut(s) 112, 142, 148, 154, 809
BciT130I CCWGG 1 cut(s) 828
BcnI CCSGG 5 cut(s) 22, 143, 246, 247, 288
BfmI CTRYAG 1 cut(s) 522
BisI GCNGC 4 cut(s) 100, 435, 612, 848
BlsI GCNGC 4 cut(s) 101, 436, 613, 849
BmcAI AGTACT 2 cut(s) 136, 385
Bme1390I CCNGG 6 cut(s) 22, 143, 246, 247, 288, 828
Bme18I GGWCC 1 cut(s) 697
BmeT110I CYCGRG 1 cut(s) 245
BmgT120I GGNCC 3 cut(s) 289, 355, 697
BmrFI CCNGG 6 cut(s) 22, 143, 246, 247, 288, 828
BmsI GCATC 3 cut(s) 156, 618, 640
BpiI GAAGAC 2 cut(s) 255, 519
BpmI CTGGAG 1 cut(s) 105
Bpu10I CCTNAGC 1 cut(s) 589
BpuEI CTTGAG 3 cut(s) 278, 493, 693
BpuMI CCSGG 5 cut(s) 22, 143, 246, 247, 288
BsaAI YACGTR 1 cut(s) 744
BsaJI CCNNGG 1 cut(s) 245
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 149, 191
Bse118I RCCGGY 1 cut(s) 60
Bse1I ACTGG 3 cut(s) 79, 561, 858
BseBI CCWGG 1 cut(s) 828
BseDI CCNNGG 1 cut(s) 245
BseGI GGATG 5 cut(s) 159, 165, 171, 492, 862
BseLI CCNNNNNNNGG 3 cut(s) 148, 149, 191
BseMII CTCAG 1 cut(s) 603
BseNI ACTGG 3 cut(s) 79, 561, 858
BseRI GAGGAG 2 cut(s) 347, 365
BseXI GCAGC 2 cut(s) 111, 598
BshFI GGCC 2 cut(s) 291, 357
BsiHKCI CYCGRG 1 cut(s) 245
BsiSI CCGG 5 cut(s) 21, 61, 143, 246, 287
BslFI GGGAC 2 cut(s) 262, 691
BslI CCNNNNNNNGG 3 cut(s) 148, 149, 191
BsmFI GGGAC 2 cut(s) 262, 691
BsnI GGCC 2 cut(s) 291, 357
BsoBI CYCGRG 1 cut(s) 245
BspACI CCGC 3 cut(s) 434, 499, 848
BspANI GGCC 2 cut(s) 291, 357
BspCNI CTCAG 1 cut(s) 602
BsrFI RCCGGY 1 cut(s) 60
BsrI ACTGG 3 cut(s) 79, 561, 858
BssAI RCCGGY 1 cut(s) 60
BssECI CCNNGG 1 cut(s) 245
Bst2UI CCWGG 1 cut(s) 828
Bst4CI ACNGT 2 cut(s) 227, 457
Bst6I CTCTTC 1 cut(s) 776
BstBAI YACGTR 1 cut(s) 744
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 2 cut(s) 589, 909
BstF5I GGATG 5 cut(s) 159, 165, 171, 492, 862
BstMWI GCNNNNNNNGC 1 cut(s) 837
BstNI CCWGG 1 cut(s) 828
BstSCI CCNGG 6 cut(s) 20, 141, 244, 245, 286, 826
BstSFI CTRYAG 1 cut(s) 522
BstSNI TACGTA 1 cut(s) 744
BstV1I GCAGC 2 cut(s) 111, 598
BstV2I GAAGAC 2 cut(s) 255, 519
BsuRI GGCC 2 cut(s) 291, 357
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 5 cut(s) 159, 165, 171, 492, 862
BtsI GCAGTG 1 cut(s) 665
BtsIMutI CAGTG 2 cut(s) 665, 851
Cac8I GCNNGC 1 cut(s) 66
Cfr10I RCCGGY 1 cut(s) 60
Cfr13I GGNCC 3 cut(s) 289, 355, 697
Cfr9I CCCGGG 1 cut(s) 245
Csp6I GTAC 5 cut(s) 135, 284, 384, 421, 745
CviAII CATG 7 cut(s) 32, 202, 208, 214, 299, 607, 814
CviQI GTAC 5 cut(s) 135, 284, 384, 421, 745
DdeI CTNAG 2 cut(s) 589, 909
DrdI GACNNNNNNGTC 1 cut(s) 193
DseDI GACNNNNNNGTC 1 cut(s) 193
Eam1104I CTCTTC 1 cut(s) 776
EarI CTCTTC 1 cut(s) 776
Eco105I TACGTA 1 cut(s) 744
Eco47I GGWCC 1 cut(s) 697
Eco57I CTGAAG 1 cut(s) 531
Eco88I CYCGRG 1 cut(s) 245
EcoO109I RGGNCCY 1 cut(s) 355
EcoRII CCWGG 1 cut(s) 826
EcoT22I ATGCAT 2 cut(s) 633, 866
FaeI CATG 7 cut(s) 35, 205, 211, 217, 302, 610, 817
FaqI GGGAC 2 cut(s) 262, 691
FatI CATG 7 cut(s) 31, 201, 207, 213, 298, 606, 813
Fnu4HI GCNGC 4 cut(s) 100, 435, 612, 848
FokI GGATG 5 cut(s) 166, 172, 178, 499, 869
Fsp4HI GCNGC 4 cut(s) 100, 435, 612, 848
GluI GCNGC 4 cut(s) 100, 435, 612, 848
GsuI CTGGAG 1 cut(s) 105
HaeIII GGCC 2 cut(s) 291, 357
HapII CCGG 5 cut(s) 21, 61, 143, 246, 287
Hin1II CATG 7 cut(s) 35, 205, 211, 217, 302, 610, 817
HincII GTYRAC 2 cut(s) 220, 242
HindII GTYRAC 2 cut(s) 220, 242
HindIII AAGCTT 1 cut(s) 711
HinfI GANTC 4 cut(s) 11, 112, 278, 563
HpaII CCGG 5 cut(s) 21, 61, 143, 246, 287
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 2 cut(s) 220, 242
Hpy188I TCNGA 3 cut(s) 106, 117, 186
Hpy188III TCNNGA 2 cut(s) 84, 805
Hpy8I GTNNAC 2 cut(s) 220, 242
HpyAV CCTTC 3 cut(s) 526, 541, 659
HpyCH4III ACNGT 2 cut(s) 227, 457
HpyCH4IV ACGT 1 cut(s) 743
HpyCH4V TGCA 6 cut(s) 169, 233, 614, 631, 658, 864
HpyF10VI GCNNNNNNNGC 1 cut(s) 837
HpyF3I CTNAG 2 cut(s) 589, 909
HpySE526I ACGT 1 cut(s) 743
Hsp92II CATG 7 cut(s) 35, 205, 211, 217, 302, 610, 817
LmnI GCTCC 3 cut(s) 86, 334, 598
Lsp1109I GCAGC 2 cut(s) 111, 598
LweI GCATC 3 cut(s) 156, 618, 640
MaeII ACGT 1 cut(s) 743
MaeIII GTNAC 1 cut(s) 750
MboII GAAGA 3 cut(s) 255, 524, 763
MluCI AATT 4 cut(s) 124, 234, 344, 365
MlyI GAGTC 1 cut(s) 20
MmeI TCCRAC 1 cut(s) 209
Mph1103I ATGCAT 2 cut(s) 633, 866
MseI TTAA 4 cut(s) 717, 765, 794, 833
MslI CAYNNNNRTG 2 cut(s) 206, 212
MspI CCGG 5 cut(s) 21, 61, 143, 246, 287
MspR9I CCNGG 6 cut(s) 22, 143, 246, 247, 288, 828
MvaI CCWGG 1 cut(s) 828
MwoI GCNNNNNNNGC 1 cut(s) 837
NciI CCSGG 5 cut(s) 22, 143, 246, 247, 288
NlaIII CATG 7 cut(s) 35, 205, 211, 217, 302, 610, 817
NsiI ATGCAT 2 cut(s) 633, 866
PcsI WCGNNNNNNNCGW 1 cut(s) 279
PfeI GAWTC 3 cut(s) 112, 278, 563
PkrI GCNGC 4 cut(s) 101, 436, 613, 849
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
Ppu21I YACGTR 1 cut(s) 744
Psp6I CCWGG 1 cut(s) 826
PspGI CCWGG 1 cut(s) 826
PspPI GGNCC 3 cut(s) 289, 355, 697
RsaI GTAC 5 cut(s) 136, 285, 385, 422, 746
RsaNI GTAC 5 cut(s) 135, 284, 384, 421, 745
RseI CAYNNNNRTG 2 cut(s) 206, 212
SaqAI TTAA 4 cut(s) 717, 765, 794, 833
SatI GCNGC 4 cut(s) 100, 435, 612, 848
Sau96I GGNCC 3 cut(s) 289, 355, 697
ScaI AGTACT 2 cut(s) 136, 385
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 6 cut(s) 22, 143, 246, 247, 288, 828
SfaNI GCATC 3 cut(s) 156, 618, 640
SfcI CTRYAG 1 cut(s) 522
SinI GGWCC 1 cut(s) 697
SmaI CCCGGG 1 cut(s) 247
SmiMI CAYNNNNRTG 2 cut(s) 206, 212
SmlI CTYRAG 3 cut(s) 293, 472, 708
SmoI CTYRAG 3 cut(s) 293, 472, 708
SnaBI TACGTA 1 cut(s) 744
Sse9I AATT 4 cut(s) 124, 234, 344, 365
SsiI CCGC 3 cut(s) 434, 499, 848
SspI AATATT 1 cut(s) 763
StyD4I CCNGG 6 cut(s) 20, 141, 244, 245, 286, 826
TaaI ACNGT 2 cut(s) 227, 457
TaiI ACGT 1 cut(s) 746
TaqI TCGA 1 cut(s) 14
TasI AATT 4 cut(s) 124, 234, 344, 365
TatI WGTACW 2 cut(s) 134, 383
TauI GCSGC 2 cut(s) 437, 850
TfiI GAWTC 3 cut(s) 112, 278, 563
Tru1I TTAA 4 cut(s) 717, 765, 794, 833
Tru9I TTAA 4 cut(s) 717, 765, 794, 833
TscAI CASTG 2 cut(s) 665, 858
TseI GCWGC 2 cut(s) 99, 611
TspDTI ATGAA 5 cut(s) 243, 390, 417, 555, 653
TspGWI ACGGA 1 cut(s) 271
TspMI CCCGGG 1 cut(s) 245
TspRI CASTG 2 cut(s) 665, 858
VpaK11BI GGWCC 1 cut(s) 697
XapI RAATTY 1 cut(s) 365
XcmI CCANNNNNNNNNTGG 1 cut(s) 691
XmaI CCCGGG 1 cut(s) 245
ZrmI AGTACT 2 cut(s) 136, 385
Zsp2I ATGCAT 2 cut(s) 633, 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.