AT1G64790

Translational activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004626
Physical Location & Seq
Reverse (-)
24064822 .. 24083428
18607 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G64790.2

Sequence Viewer

Length: 7833 bp
ATGGCTTCTCCGTTGGAGTCTCTTCTCTCCATCTCTGGCTCTGTCTCTACAAGCTCCACTTTAATCAGACTCCGGATCTTCCGCCATGATATCCCCGAGATTCTCCAGAATTCCGATATGACGTCTGATATTGCGCCTGTTATAGTTGACATGATATTCCAAACATTGGCTATATATGATGACCGTGCCTCACGGAAAGCTGTAGATGATTTGATTGTAAAGGGACTGGGAAATGTTACCTTCATGAAAACTTTTGCGGCCATGCTTGTTCAAGTTATGGAAAAGCAGTTGAAATTTTGTTTTGATACAGTCTGTTACAGACTACTAATTTGGTCATGTCTTCTTCTGGAAAAAAGTCAATTCGCTACTGTTTCGAAGAATGCATTTGTTAGAGTTGCATCAACGCAGGCTTCATTACTTCGTATCATTATGGAGAGCTCTTTCCGTATGCGGAGGGCTTGTAAAAGATTTATGTTCCATTTGTTCTCTCAGTCACAAGCTATCTACAGTTTATATATGGATGAAGTTAAAGGTTCACGGATTCCGTACAAGGATAGCCCTGAGTTATTAGGACTACTGCTTGAGTTCTCTTGTTCGTCTCCTGCTCTATTTGAACAGTCAAAGGCCATATTTGTTGATATCTATGTTAAAGATGTCCTGAATTCTAGAGAAAAGCAGAAACCAAATCTTAGTAATTGTTTCAAACCTCTTCTTCAACGCTTGTCACATGAGGAATTCCAAACTGTCATCCTTCCTGCAGCTGTTAAAATGTTGAAGCGTAATCCAGAAATTGTCCTGGAATCAGTTGGATTTCTATTAGCAAATGTAAACATTGATCTAAGTAAGTATGCCCTGGAACTGCTACCAGTTATACTACCACAGGCTCGGCATACGGATGAAGACAGGCGTCTAGGAGCTTTATCAATGGTTATGTGCTTAAGTGAAAAATCAAGTAATCCTGATACTATAGAAGCAATGTTTGCGTCAGTTAAAGCCATAATTGGAGGTTCTGAAGGAAGACTTCAATCTCCCCATCAAAGAATTGGAATGCTGAATGCAGTGCAAGAACTGGCAAGTGCTCCTGAAGGAAAGTATATTGGTAGTTTGTCGAGGACGATATGCAGCTTTCTCATAGCCTGCTACAAGGATGAAGGAAATGAAGACGTAAAACTTTCAATTTTGTCTGCTGTAGCTTCATGGGCATCACGATCTTCTGTTGCCATCCAACCAAACTTAGTATCTTTCATTGCTGCCGGCCTTAAGGAGAAAGAAGCTCTAAGGAGGGGGCATCTCCGCTGTGTACGAATCATATGCAGAAATCCCGATACCATATCACAGATATCAGACTTGCTATCCCCTTTAATTCAACTTGTAAAGACGGGATTTACAAAGGCAGTACAACGTCTGGATGGGATATATGCGCTACTGATCGTTTCCAAAATTGCTGCCTGTGACATCAAAGCAGAGGATACTATGGTGAAGGAGAAATTATGGACTTTGATATCTCAAAATGAGCCTTCACTTGTGCAAATTACTTTGGCCTCAAAACTTTCAAGTGATGATTGTGTAGTATGTGTAGACCTTCTTGAGGTGCTCCTTGTGGAACACTCCTCCAGGGTTTTGGAAGCATTTTCATTGAAATCACTATCACAGTTGCTTCTCTTTCTGCTTTGCCATCCAAGCTGGAATGTTCGTAAAACAGCTTACAATTCTGTTACGAAGATCTTCCTCGCGACTTCACAGCTAGCCACTACTCTTTTAGACGAGTTCTCGGATTTTCTTTCCATAACTGGGGACCAAATCGTTTCTTCTAGAACCAGTGACGCAGATAATCCCGCGGATCATCAAGCTCCTTTCGTCCCCTCTGTAGAGGTGTTAGTGAAGGCTTTGATTGTCATTTCATCGGCAGCAGTTGCTGGACCTCCAAGCTCATGGATTGTTCGTGCCATATTTTGCTCGCATCATCCCAGCATAGTTGGTACTGGAAAAAGAGATGCTGTTTGGAAGAGATTGCAAAAGTGTCTCAAAACTTGTGGTTTTGATGTTGCTACTTTTTTGTCTACCAATGGGGAAAGTGTTTGCAAGAGTCTACTTGGACCAATGGGTTTAACGAGTGCTAAAACTCCGGAGCAGCAAGCGGCCGTATATTCCTTGTCCACCATGATGTCTCTGGCACCAGAGGATACATTCACAGTGTTCAAGATGCATCTACAAGACCTTCCAGATCGACTGTCTCATGATATGCTCTCAGAGACTGACATTAAGATCTTTCATACACCTGAGGGGATGCTTTTAAGTGAGCAAGGTGTCTATGTTGCTCAGACAATTGGTGCCAAGTATACAAAGCAGGAGCCGAGTTCTAATCATTCTCTAAAGAAAGGATTGGCTAGTAGAGAAACTGCAAACTCAGGGAGAAGGGACACTGCAAAGTTGACAAAGAAAGCTGACAAAGGAAAAACGGCAAAGGAAGAGGCACGGGAACTGATGCTCAAAGAGGAGGCCTCAACACGGGAAAATGTTCACAGGATACAAAAAAGTCTTTCGTTGGTGCTTCATGCCCTTGGGGAGATGGGTCTTGCTAATCCTGTGTTTTGTCACAGCCAGTTACCTTTTTTGGCTACTTTTTTGGATCCATTGCTGCGATCACCTATTGTAAGTGCTGCAGCCTTTGAAAACTTGGTGAAGCTTGCACGTTGTACAGTTCAGCCACTGTGTAACTGGGCTCTAGAAATTTCAACTGCTCTGCGTTTGATCGCGATTGATGAAGTTGATACTTCATTTGATTTTCGTCCATCAGTTGACAAGGCTGGGAAAACATATGAGGGTCTTTTTGAGAGGATTGTAAATGGGTTATCGATATCTTGTAAATCAGGACCACTTCCTGTAGATACATTCACGTTCATTTTCCCGATACTGGAGCGAATTTTACTGTCATCAAAGCGGACAAAACTCCATGATGATGTGCTGCAGATTTTATACATGCATCTAGATCCGATGCTGCCACTTCCAAGACTCCGGATGATATCAGTTCTATATCATGTGCTAGGTGTTGTTCCCGCGTATCAAGCATCAGTTGGACCTGCACTGAATGAATTGTGTCTTGGATTGCAAGCAGATGATGTCGCAAATGCGCTCTATGGAGTCTATTCAAAAGATGTTCATGTCAGACTCGCCTGCTTAAACGCTGTGAAGTGTATTCCAGCTGTTTCCAAATGCTCCCTTCCTCAGAATGTTAAAATAGCAACAAACATTTGGATAGCTCTACATGACCCTGAAAAGTCAGTGGCTGAATCAGCAGATGATTTATGGGCCCGCTATGGTCATGATTTGGGGACTGATTATTCAGGAATCTTTAAAGCACTTTCTCATATAAATCTCAACGTCCGTTTAGCTGCAGCAGAAGCACTGGCTGATGCTCTTCATGAAAGCCCGTCATCAATCCAGTTATCCCTTTCAACTTTATTTTCTCTGTACATCCGTGATGCTACGTCTGGTGAGGATGTCTTTGATGCTGGTTGGATAGGAAGGCAAGGAATTGCCTTAGCACTGCAATCTGCTGCTGACGTATTGACAACAAAGGACCTTCCTGCTGTTATGACCTTTTTGATATCCCGCGCACTGGCTGATCCCAACACAGATGTTCGTGGAAAGATGATCAATGCTGGTATCATGATTATTGATAAGCATGGCAAAGAAAACGTTTCATTGCTGTTCCCCATCTTTGAGAATTATTTAAATAAAGAGGCATCTGATGAAGAGGAATATGACTTGGTTCGTGAGGGTGTTGTTATATTCACTGGAGCACTAGCAAAGCACTTAGCAAGAGATGATCCTAAAGTACATAATGTTGTGGAAAAGCTGTTGGAGGTGTTGAATACACCATCAGAATCTGTGCAACGTGCTGTCTCGACATGCCTTTCTCCACTTGTGTTATCAAAGCAAGAGGAGGCCCCTGCCCTTTTTTTGAGGCTCTTGGATAAGCTAATGAAGAGTGATAAATATGGTGAGCGTAGAGGAGCAGCTTTTGGTCTTGCGGGAGTAGTTATGGGCTTTGGAATTTCTAGCTTGAAGAAATATGGTCTTATAGTCACTTTGCAAGAAGCACTTATTGACAGAAATTCTGCTAAACGTCGTGAAGGAGCATTACTCGCATTTGAATGCCTCTGCGAGAAGCTTGGAAAATTATTTGAACCGTATGTGATAAAGATGTTACCCTTACTTCTGGTGTCGTTCTCTGACCAAGTCGGAGCAGTTCGCGAAGCAGCAGAATGTGCTGCTCGAGCTATGATGTCTCAACTATCTGCATATGGTGTGAAACTTGTCCTTCCTTCTCTACTCAAGGGACTTGAAGATAAAGCATGGAGAACAAAGCAAAGCAGTGTACAGCTCCTCGGTGCTATGGCATTTTGTGCTCCTCAGCAACTGTCCCAATGTCTTCCTAGGGTTGTGCCAAAACTTACAGAGGTTTTGACGGATACACATCCAAAAGTCCAGTCAGCCGGGCAGTTAGCACTTCAGCAGGTTGGAAGTGTCATAAAGAACCCAGAGATATCCTCTCTTGTCCCCACCCTTCTATTGGCCTTAACAGATCCCAATGAGTACACACGCCATGCTCTTGACACCCTTCTTCAAACAACATTCGTTAATTCTGTTGATGCCCCCTCGCTTGCACTGTTGGTACCAATAGTACATAGAGGATTGAGAGAAAGAAGTTCTGAAACAAAAAAGAAAGCTTCTCAAATTGTCGGTAATATGTGTTCTTTGGTGACAGAGCCAAAGGATATGATTCCTTACATAGGATTGCTCCTTCCTGAGGTTAAAAAGGTTCTTGTGGATCCAATTCCCGAAGTACGTTCTGTTGCAGCAAGGGCTGTTGGGTCACTTATTAGAGGAATGGGAGAGGATAATTTCCCAGATCTTGTTCCGTGGCTGTTTGAAACTCTAAAATCTGATACAAGCAATGTTGAAAGATATGGAGCCGCTCAGGGATTAAGTGAGGTTATAGCAGCTCTTGGGACAGATTACTTTGAGAATATACTTCCTGACCTCATTCGTCATTGTTCTCATCAAAAAGCATCGGTCCGAGATGGATACCTTACGCTTTTCAAGTTCTTGCCAAGATCCTTGGGTGCTCAGTTCCAGAAGTACTTGCAGCTTGTTTTACCGGCCATTCTGGATGGTCTTGCCGATGAGAATGAATCTGTTCGTGATGCAGCCCTGGGGGCTGGCCATGTACTGGTGGAGCACCATGCAACAACGTCTCTGCCTCTTCTTCTTCCAGCTGTGGAGGATGGTATTTTCAATGATAATTGGCGTATCCGTCAAAGTTCTGTTGAACTGCTTGGGGATCTATTGTTCAAGGTTGCTGGTACATCGGGCAAAGCTCTACTGGAGGGTGGAAGTGATGATGAAGGTGCAAGTACTGAAGCGCAGGGTCGTGCCATTATTGATATTTTAGGAATGGACAAGCGCAATGAAGTTCTTGCAGCGTTATATATGGTCCGGACTGATGTTAGTTTGTCTGTTCGTCAGGCGGCACTACATGTATGGAAAACCATCGTAGCAAATACACCAAAGACGTTGAAGGAAATAATGCCAATTTTGATGAGTACCTTAATTTCTTCTTTGGCATCACCATCTTCTGAGAGGCGCCAGGTAGCAGGAAGATCACTTGGAGAGCTGGTTAGGAAGCTTGGTGAAAGGGTGCTTCCTCTTATCATTCCAATTTTATCTAAAGGACTGAAAGACCCAGATGTTGACAAAAGACAAGGTGTATGCATTGGCCTGAATGAAGTGATGGCAAGTGCTGGAAGGAGTCAGTTGCTGAGTTTCATGGATCAGCTTATTCCCACAATTAGGACAGCACTTTGTGATAGTGCTCTCGAGGTTCGGGAATCTGCTGGACTAGCATTCAGCACTCTGTATAAGAGTGCTGGACTGCAAGCAATGGATGAGATTATTCCAACTCTTTTGGAAGCCTTAGAAGATGATGAAATGTCTACTACTGCCTTGGATGGACTTAAACAAATTATAAGTGTTAGGACAGCAGCTGTTCTTCCACACATCTTGCCCAAGCTTGTCCATCTTCCTCTCTCTGCCTTAAATGCTCATGCTTTGGGAGCCTTAGCTGAGGTTGCTGGTGCTGGTTTTAACACGCATCTTGGGACCATACTTCCTGCTTTGCTCTCTGCAATGGGAGGTGAAAACAAGGAAGTCCAAGAATTGGCACAAGAGGCCGCAGAGAGAGTGGTGCTTGTCATTGATGAAGAAGGCGTTGAGACTCTATTATCAGAACTTCTCAAAGGAGTGTCTGATAGTCAGGCTTCAATTAGAAGAAGTTCTGCATATCTGATTGGTTACTTCTTCAAGAGTAGCAAGCTTTACTTAATTGATGAAGCTCCAAACATGATATCTACCTTGATTGTTATGCTCAGTGATTCTGATTCAACAACGGTTGCGGTTTCCTGGGAAGCTTTGGCTAGGGTTATTGGTTCAGTTCCAAAGGAAGTACTTCCTTCATATATAAAACTTGTACGTGATGCTGTATCTACAGCTAGGGACAAAGAGCGCAGGAAGCGTAAGGGAGGATATGTTGTTATTCCTGGTCTATGCCTCCCCAAGTCCCTTAAGCCATTACTTCCAGTATTTCTACAGGGTCTAATAAGTGGATCTGCTGAACTAAGAGAGCAAGCTGCAATTGGTCTTGGTGAACTTATTGAAGTTACTAGCGAGCAAGCTTTGAAGGAGTTCGTCATACCTATAACAGGGCCTCTTATTCGAATCATTGGAGACCGGTTTCCATGGCAAGTCAAAAGTGCTATCTTGGCTACATTAATCATCCTAATACAAAGGGGTGGGATGGCGCTAAAGCCATTCCTCCCTCAACTGCAAACAACATTTGTCAAGTGTCTGCAAGACTCTACAAGGACCATCCGTTCTAGTGCTGCGGTTGCCCTGGGAAAGCTTAGTGCATTAAGCACAAGGATTGACCCATTAGTTGGTGATTTGATGACAAGTTTCCAGGCTGCAGATTCTGGGGTACGTGAGGCCATCCTATCTGCAATGAGAGGTGTAATAAAGCATGCTGGGAAGAGCATTGGGCCTGCTGTTAGAGTACGCATTTTTGATCTCCTAAAGGATCTAATGCACCATGAGGATGACCAAGTTCGAATATCTGCCACAAGCATGCTGGGTGTTTTATCACAGTACTTGGAAGCTGCACAACTCAGTGTTCTGCTTCAAGAGGTCAATGATTTATCTGCTTCGCAAAATTGGGGTGCTAGGCATGGCTCAGTTCTCTGCATTTCATCCTTGCTGAAACACAATCCTTCCACCATTATGACGTCATCGCTCTTTTCCTCTATGTTAAATTCTCTTAAGAGCTCTTTGAAGGATGAGAAGTTTCCATTACGTGAAAGCTCGACAAAGGCCTTGGGAAGGCTTTTGCTAAAACAACTTGCGACGGATCCTTCCAACACAAAAGTGGTTATCGATGTCCTTTCATCGATTGTCTCAGCTTTGCATGATGATTCGAGTGAGGTTCGAAGAAGAGCACTCTCTTCACTTAAAGCTTTTGCAAAAGATAATCCATCAGCAACCATGGCAAATATAAGTGTTATCGGACCTCCTCTAGCTGAATGTTTGAAGGATGGGAACACACCAGTGAGACTTGCTGCAGAAAGATGTGCTTTGCACGTGTTCCAATTAACAAAAGGCGCAGAAAATGTTCAAGCAGCTCAAAAATACATTACAGGATTGGATGCCCGACGGTTATCCAAGTTTCCTGAACAAAGCGATGACAGTGAAAGCGATGATGATAATGTAAGTGGGTAG

Protein Analysis

2610

Amino Acids

284.78

Weight (kDa)

6.77

Isoelectric Point (pI)

40.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GNC1_N PF24993 327 - 460 2.8e-41 Stalled ribosome sensor GCN1 N-terminal
HEAT_EF3_N PF24987 1417 - 1588 7.7e-43 EF3 N-terminal HEAT repeat
HEAT_GCN1 PF23271 1464 - 1610 2e-08 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_GCN1 PF23271 1601 - 1741 1.3e-06 Stalled ribosome sensor GCN1-like, HEAT repeats
TPR_DNAAF5 PF25757 1601 - 1825 2.5e-06 Dynein axonemal assembly factor 5-like, TPR repeats
HEAT_EF3_GNC1 PF24984 1628 - 1714 3.5e-27 Elongation factor 3/GNC1 HEAT repeat
HEAT_GCN1 PF23271 1702 - 1849 1.7e-08 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_N PF24987 1745 - 1872 3e-08 EF3 N-terminal HEAT repeat
HEAT_GCN1 PF23271 2104 - 2293 9.9e-51 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_N PF24987 2119 - 2233 2.9e-07 EF3 N-terminal HEAT repeat
XMAP215_CLASP_TOG PF21041 2385 - 2501 8.7e-06 XMAP215/Dis1/CLASP, TOG domain
HEAT_GCN1_C_2 PF25801 2416 - 2667 2.1e-33 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_GCN1_C PF25786 2514 - 2668 1.1e-72 GCN1-like, C-terminal HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 6032
AatII GACGTC 2 cut(s) 125, 7346
Acc36I ACCTGC 2 cut(s) 3088, 4510
Acc65I GGTACC 1 cut(s) 4678
AccB1I GGYRCC 4 cut(s) 2173, 2330, 4678, 5649
AccB7I CCANNNNNTGG 4 cut(s) 166, 5236, 6223, 6998
AccBSI CCGCTC 1 cut(s) 4982
AccI GTMKAC 5 cut(s) 1578, 2060, 2089, 2339, 5999
AccII CGCG 6 cut(s) 1733, 1838, 2756, 3059, 3615, 4257
AccIII TCCGGA 4 cut(s) 72, 2125, 3015, 5502
AclI AACGTT 1 cut(s) 3699
AcoI YGGCCR 4 cut(s) 258, 2139, 5166, 5227
AcsI RAATTY 9 cut(s) 109, 293, 661, 734, 2730, 2922, 4056, 4117, 7369
AcuI CTGAAG 4 cut(s) 1032, 1104, 4499, 5445
AcvI CACGTG 1 cut(s) 7696
AcyI GRCGYC 4 cut(s) 122, 907, 5650, 7343
AdeI CACNNNGTG 1 cut(s) 5870
AflII CTTAAG 4 cut(s) 937, 1259, 6626, 7376
AflIII ACRYGT 2 cut(s) 5542, 7695
AgeI ACCGGT 1 cut(s) 6792
AjnI CCWGG 9 cut(s) 795, 852, 1613, 5217, 5652, 6464, 6601, 6954, 7020
AjuI GAANNNNNNNTTGG 2 cut(s) 3084, 3116
AleI CACNNNNGTG 2 cut(s) 7481, 7661
AloI GAACNNNNNNTCC 4 cut(s) 5339, 5371, 6919, 6951
AlwNI CAGNNNCTG 9 cut(s) 1916, 2255, 2710, 3287, 3890, 4423, 5824, 6050, 7034
Ama87I CYCGRG 3 cut(s) 95, 4278, 5882
Aor13HI TCCGGA 4 cut(s) 72, 2125, 3015, 5502
ApaI GGGCCC 1 cut(s) 3313
ApoI RAATTY 9 cut(s) 109, 293, 661, 734, 2730, 2922, 4056, 4117, 7369
ArsI GACNNNNNNTTYG 4 cut(s) 312, 344, 4478, 4510
AseI ATTAAT 1 cut(s) 6833
AsiGI ACCGGT 1 cut(s) 6792
Asp700I GAANNNNTTC 7 cut(s) 1724, 2517, 5202, 6337, 6510, 6746, 7725
Asp718I GGTACC 1 cut(s) 4678
AspA2I CCTAGG 1 cut(s) 4439
AsuC2I CCSGG 1 cut(s) 4501
AsuII TTCGAA 4 cut(s) 374, 6778, 7168, 7543
AsuNHI GCTAGC 1 cut(s) 1744
AvaI CYCGRG 3 cut(s) 95, 4278, 5882
AvrII CCTAGG 1 cut(s) 4439
AxyI CCTNAGG 2 cut(s) 2280, 4812
BaeGI GKGCMC 1 cut(s) 3313
BaeI ACNNNNGTAYC 2 cut(s) 1461, 1494
BalI TGGCCA 1 cut(s) 5229
BamHI GGATCC 3 cut(s) 2629, 4834, 7465
BanI GGYRCC 4 cut(s) 2173, 2330, 4678, 5649
BanII GRGCYC 4 cut(s) 440, 2725, 3313, 7385
BarI GAAGNNNNNNTAC 2 cut(s) 4128, 4160
BbrPI CACGTG 1 cut(s) 7696
BbsI GAAGAC 5 cut(s) 332, 906, 1024, 1167, 4427
BbvCI CCTCAGC 2 cut(s) 4416, 6129
BceAI ACGGC 2 cut(s) 2126, 2475
BcgI CGANNNNNNTGC 2 cut(s) 1293, 1327
BciT130I CCWGG 9 cut(s) 797, 854, 1615, 5219, 5654, 6466, 6603, 6956, 7022
BciVI GTATCC 6 cut(s) 1462, 2176, 2520, 4468, 5084, 5327
BclI TGATCA 1 cut(s) 3654
BcnI CCSGG 1 cut(s) 4501
BfoI RGCGCY 2 cut(s) 5653, 6866
BfrI CTTAAG 4 cut(s) 937, 1259, 6626, 7376
BfuAI ACCTGC 2 cut(s) 3088, 4510
BfuI GTATCC 6 cut(s) 1462, 2176, 2520, 4468, 5084, 5327
BglI GCCNNNNNGGC 1 cut(s) 5222
BglII AGATCT 3 cut(s) 1722, 2265, 4915
BlnI CCTAGG 1 cut(s) 4439
BmcAI AGTACT 4 cut(s) 5147, 5422, 6510, 7208
BmeT110I CYCGRG 3 cut(s) 95, 4278, 5882
BmrI ACTGGG 3 cut(s) 236, 1800, 2728
BmtI GCTAGC 1 cut(s) 1748
BmuI ACTGGG 3 cut(s) 236, 1800, 2728
BoxI GACNNNNGTC 1 cut(s) 906
BpiI GAAGAC 5 cut(s) 332, 906, 1024, 1167, 4427
BplI GAGNNNNNCTC 6 cut(s) 2486, 2518, 4131, 4163, 4538, 4570
BpmI CTGGAG 5 cut(s) 89, 1597, 2936, 3819, 5411
Bpu10I CCTNAGC 5 cut(s) 3541, 4416, 4983, 6124, 6129
Bpu14I TTCGAA 4 cut(s) 374, 6778, 7168, 7543
BpuEI CTTGAG 3 cut(s) 602, 1607, 4322
BpuMI CCSGG 1 cut(s) 4501
Bsa29I ATCGAT 3 cut(s) 2855, 7491, 7505
BsaAI YACGTR 4 cut(s) 6536, 7043, 7412, 7696
BsaBI GATNNNNATC 1 cut(s) 4479
BsaHI GRCGYC 4 cut(s) 122, 907, 5650, 7343
BsaI GGTCTC 1 cut(s) 6783
BsaWI WCCGGW 5 cut(s) 72, 2125, 3015, 5502, 6792
BsaXI ACNNNNNCTCC 6 cut(s) 445, 475, 5667, 5697, 6576, 6606
Bse118I RCCGGY 3 cut(s) 1253, 5164, 6792
Bse21I CCTNAGG 2 cut(s) 2280, 4812
Bse3DI GCAATG 9 cut(s) 981, 1245, 2633, 3704, 4966, 5479, 5952, 6198, 7068
Bse8I GATNNNNATC 1 cut(s) 4479
BseAI TCCGGA 4 cut(s) 72, 2125, 3015, 5502
BseBI CCWGG 9 cut(s) 797, 854, 1615, 5219, 5654, 6466, 6603, 6956, 7022
BseCI ATCGAT 3 cut(s) 2855, 7491, 7505
BseJI GATNNNNATC 1 cut(s) 4479
BseMI GCAATG 9 cut(s) 981, 1245, 2633, 3704, 4966, 5479, 5952, 6198, 7068
BseRI GAGGAG 7 cut(s) 1600, 2510, 3959, 4029, 4379, 4404, 7617
BseSI GKGCMC 1 cut(s) 3313
BseX3I CGGCCG 1 cut(s) 2139
BseYI CCCAGC 4 cut(s) 1967, 2807, 7085, 7189
BsgI GTGCAG 2 cut(s) 3066, 7203
Bsh1236I CGCG 6 cut(s) 1733, 1838, 2756, 3059, 3615, 4257
Bsh1285I CGRYCG 1 cut(s) 2142
BshNI GGYRCC 4 cut(s) 2173, 2330, 4678, 5649
BshTI ACCGGT 1 cut(s) 6792
BshVI ATCGAT 3 cut(s) 2855, 7491, 7505
BsiEI CGRYCG 1 cut(s) 2142
BsiHKCI CYCGRG 3 cut(s) 95, 4278, 5882
BsiSI CCGG 8 cut(s) 73, 1254, 2126, 3016, 4500, 5165, 5503, 6793
BsmBI CGTCTC 2 cut(s) 603, 5265
BsmI GAATGC 5 cut(s) 385, 1053, 1060, 4163, 5909
Bso31I GGTCTC 1 cut(s) 6783
BsoBI CYCGRG 3 cut(s) 95, 4278, 5882
Bsp119I TTCGAA 4 cut(s) 374, 6778, 7168, 7543
Bsp120I GGGCCC 1 cut(s) 3309
Bsp13I TCCGGA 4 cut(s) 72, 2125, 3015, 5502
Bsp1407I TGTACA 3 cut(s) 2696, 3471, 4381
Bsp19I CCATGG 2 cut(s) 6800, 7599
Bsp68I TCGCGA 3 cut(s) 1733, 2756, 4257
BspDI ATCGAT 3 cut(s) 2855, 7491, 7505
BspEI TCCGGA 4 cut(s) 72, 2125, 3015, 5502
BspFNI CGCG 6 cut(s) 1733, 1838, 2756, 3059, 3615, 4257
BspHI TCATGA 5 cut(s) 243, 2236, 3322, 3421, 3669
BspMAI CTGCAG 6 cut(s) 760, 2665, 2970, 3397, 7030, 7678
BspMI ACCTGC 2 cut(s) 3088, 4510
BspOI GCTAGC 1 cut(s) 1748
BspQI GCTCTTC 3 cut(s) 3423, 7085, 7543
BspT104I TTCGAA 4 cut(s) 374, 6778, 7168, 7543
BspT107I GGYRCC 4 cut(s) 2173, 2330, 4678, 5649
BspTI CTTAAG 4 cut(s) 937, 1259, 6626, 7376
BspTNI GGTCTC 1 cut(s) 6783
BsrBI CCGCTC 1 cut(s) 4982
BsrDI GCAATG 9 cut(s) 981, 1245, 2633, 3704, 4966, 5479, 5952, 6198, 7068
BsrFI RCCGGY 3 cut(s) 1253, 5164, 6792
BsrGI TGTACA 3 cut(s) 2696, 3471, 4381
BssAI RCCGGY 3 cut(s) 1253, 5164, 6792
BssNAI GTATAC 1 cut(s) 2340
BssNI GRCGYC 4 cut(s) 122, 907, 5650, 7343
BssT1I CCWWGG 7 cut(s) 2560, 4439, 5124, 6009, 6800, 7431, 7599
Bst1107I GTATAC 1 cut(s) 2340
Bst2UI CCWGG 9 cut(s) 797, 854, 1615, 5219, 5654, 6466, 6603, 6956, 7022
BstACI GRCGYC 4 cut(s) 122, 907, 5650, 7343
BstAFI CTTAAG 4 cut(s) 937, 1259, 6626, 7376
BstAPI GCANNNNNTGC 4 cut(s) 980, 1913, 4271, 4409
BstAUI TGTACA 3 cut(s) 2696, 3471, 4381
BstBAI YACGTR 4 cut(s) 6536, 7043, 7412, 7696
BstBI TTCGAA 4 cut(s) 374, 6778, 7168, 7543
BstDSI CCRYGG 4 cut(s) 1836, 4925, 6800, 7599
BstENI CCTNNNNNAGG 5 cut(s) 1586, 4794, 4811, 6762, 7435
BstFNI CGCG 6 cut(s) 1733, 1838, 2756, 3059, 3615, 4257
BstH2I RGCGCY 2 cut(s) 5653, 6866
BstMCI CGRYCG 1 cut(s) 2142
BstNI CCWGG 9 cut(s) 797, 854, 1615, 5219, 5654, 6466, 6603, 6956, 7022
BstNSI RCATGY 5 cut(s) 2983, 3915, 5546, 7085, 7189
BstPAI GACNNNNGTC 1 cut(s) 906
BstSLI GKGCMC 1 cut(s) 3313
BstUI CGCG 6 cut(s) 1733, 1838, 2756, 3059, 3615, 4257
BstV2I GAAGAC 5 cut(s) 332, 906, 1024, 1167, 4427
BstXI CCANNNNNNTGG 2 cut(s) 1621, 1932
BstZ17I GTATAC 1 cut(s) 2340
BstZI CGGCCG 1 cut(s) 2139
Bsu15I ATCGAT 3 cut(s) 2855, 7491, 7505
Bsu36I CCTNAGG 2 cut(s) 2280, 4812
BsuI GTATCC 6 cut(s) 1462, 2176, 2520, 4468, 5084, 5327
BsuTUI ATCGAT 3 cut(s) 2855, 7491, 7505
BtgI CCRYGG 4 cut(s) 1836, 4925, 6800, 7599
BtgZI GCGATG 3 cut(s) 7332, 7809, 7824
BtsI GCAGTG 4 cut(s) 1065, 2421, 3545, 4384
BtuMI TCGCGA 3 cut(s) 1733, 2756, 4257
BveI ACCTGC 2 cut(s) 3088, 4510
CaiI CAGNNNCTG 9 cut(s) 1916, 2255, 2710, 3287, 3890, 4423, 5824, 6050, 7034
CciI TCATGA 5 cut(s) 243, 2236, 3322, 3421, 3669
Cfr10I RCCGGY 3 cut(s) 1253, 5164, 6792
Cfr42I CCGCGG 1 cut(s) 1839
ClaI ATCGAT 3 cut(s) 2855, 7491, 7505
CpoI CGGWCCG 1 cut(s) 5080
CseI GACGC 3 cut(s) 896, 972, 1832
CspAI ACCGGT 1 cut(s) 6792
CspCI CAANNNNNGTGG 4 cut(s) 2014, 2049, 6048, 6083
CspI CGGWCCG 1 cut(s) 5080
DinI GGCGCC 1 cut(s) 5651
DraI TTTAAA 2 cut(s) 3355, 3735
DraIII CACNNNGTG 1 cut(s) 5870
EaeI YGGCCR 4 cut(s) 258, 2139, 5166, 5227
EagI CGGCCG 1 cut(s) 2139
EciI GGCGGA 1 cut(s) 71
Ecl136II GAGCTC 2 cut(s) 438, 7383
EclXI CGGCCG 1 cut(s) 2139
Eco130I CCWWGG 7 cut(s) 2560, 4439, 5124, 6009, 6800, 7431, 7599
Eco147I AGGCCT 2 cut(s) 2501, 7430
Eco24I GRGCYC 4 cut(s) 440, 2725, 3313, 7385
Eco31I GGTCTC 1 cut(s) 6783
Eco32I GATATC 9 cut(s) 91, 640, 1341, 1503, 2859, 3024, 3609, 4551, 6411
Eco52I CGGCCG 1 cut(s) 2139
Eco53kI GAGCTC 2 cut(s) 438, 7383
Eco57I CTGAAG 4 cut(s) 1032, 1104, 4499, 5445
Eco72I CACGTG 1 cut(s) 7696
Eco81I CCTNAGG 2 cut(s) 2280, 4812
Eco88I CYCGRG 3 cut(s) 95, 4278, 5882
EcoICRI GAGCTC 2 cut(s) 438, 7383
EcoNI CCTNNNNNAGG 5 cut(s) 1586, 4794, 4811, 6762, 7435
EcoO109I RGGNCCY 3 cut(s) 3580, 3949, 6767
EcoRI GAATTC 3 cut(s) 109, 661, 734
EcoRII CCWGG 9 cut(s) 795, 852, 1613, 5217, 5652, 6464, 6601, 6954, 7020
EcoRV GATATC 9 cut(s) 91, 640, 1341, 1503, 2859, 3024, 3609, 4551, 6411
EcoT14I CCWWGG 7 cut(s) 2560, 4439, 5124, 6009, 6800, 7431, 7599
EcoT22I ATGCAT 4 cut(s) 385, 2208, 2985, 5780
EcoT38I GRGCYC 4 cut(s) 440, 2725, 3313, 7385
EgeI GGCGCC 1 cut(s) 5651
EheI GGCGCC 1 cut(s) 5651
ErhI CCWWGG 7 cut(s) 2560, 4439, 5124, 6009, 6800, 7431, 7599
Esp3I CGTCTC 2 cut(s) 603, 5265
FauI CCCGC 5 cut(s) 1843, 3064, 3320, 3620, 4027
FauNDI CATATG 3 cut(s) 1310, 2818, 4306
FbaI TGATCA 1 cut(s) 3654
FblI GTMKAC 5 cut(s) 1578, 2060, 2089, 2339, 5999
FriOI GRGCYC 4 cut(s) 440, 2725, 3313, 7385
GsaI CCCAGC 4 cut(s) 1971, 2811, 7089, 7193
GsuI CTGGAG 5 cut(s) 89, 1597, 2936, 3819, 5411
HaeII RGCGCY 2 cut(s) 5653, 6866
HapII CCGG 8 cut(s) 73, 1254, 2126, 3016, 4500, 5165, 5503, 6793
HgaI GACGC 3 cut(s) 896, 972, 1832
Hin1I GRCGYC 4 cut(s) 122, 907, 5650, 7343
HincII GTYRAC 4 cut(s) 148, 2433, 2800, 5758
HindII GTYRAC 4 cut(s) 148, 2433, 2800, 5758
HpaII CCGG 8 cut(s) 73, 1254, 2126, 3016, 4500, 5165, 5503, 6793
Hpy99I CGWCG 3 cut(s) 4134, 7465, 7770
Hsp92I GRCGYC 4 cut(s) 122, 907, 5650, 7343
KasI GGCGCC 1 cut(s) 5649
Kpn2I TCCGGA 4 cut(s) 72, 2125, 3015, 5502
KpnI GGTACC 1 cut(s) 4682
KroI GCCGGC 1 cut(s) 1253
KroNI GCCGGC 1 cut(s) 1255
Ksp22I TGATCA 1 cut(s) 3654
KspI CCGCGG 1 cut(s) 1839
LguI GCTCTTC 3 cut(s) 3423, 7085, 7543
MbiI CCGCTC 1 cut(s) 4982
MfeI CAATTG 2 cut(s) 2325, 6696
MlsI TGGCCA 1 cut(s) 5229
MluNI TGGCCA 1 cut(s) 5229
Mly113I GGCGCC 1 cut(s) 5650
MlyI GAGTC 9 cut(s) 26, 63, 2095, 3006, 3150, 3162, 5824, 6274, 6911
MmeI TCCRAC 9 cut(s) 787, 1249, 3055, 3497, 3843, 4225, 4504, 5987, 7497
Mox20I TGGCCA 1 cut(s) 5229
Mph1103I ATGCAT 4 cut(s) 385, 2208, 2985, 5780
MroI TCCGGA 4 cut(s) 72, 2125, 3015, 5502
MroNI GCCGGC 1 cut(s) 1253
MroXI GAANNNNTTC 7 cut(s) 1724, 2517, 5202, 6337, 6510, 6746, 7725
MscI TGGCCA 1 cut(s) 5229
MslI CAYNNNNRTG 9 cut(s) 894, 2162, 2958, 4156, 7155, 7184, 7337, 7481, 7661
Msp20I TGGCCA 1 cut(s) 5229
MspA1I CMGCKG 6 cut(s) 761, 1296, 1838, 3203, 5282, 6050
MspCI CTTAAG 4 cut(s) 937, 1259, 6626, 7376
MspI CCGG 8 cut(s) 73, 1254, 2126, 3016, 4500, 5165, 5503, 6793
MunI CAATTG 2 cut(s) 2325, 6696
Mva1269I GAATGC 5 cut(s) 385, 1053, 1060, 4163, 5909
MvaI CCWGG 9 cut(s) 797, 854, 1615, 5219, 5654, 6466, 6603, 6956, 7022
MvnI CGCG 6 cut(s) 1733, 1838, 2756, 3059, 3615, 4257
NaeI GCCGGC 1 cut(s) 1255
NarI GGCGCC 1 cut(s) 5650
NciI CCSGG 1 cut(s) 4501
NcoI CCATGG 2 cut(s) 6800, 7599
NdeI CATATG 3 cut(s) 1310, 2818, 4306
NgoMIV GCCGGC 1 cut(s) 1253
NheI GCTAGC 1 cut(s) 1744
NmeAIII GCCGAG 2 cut(s) 865, 2379
NmuCI GTSAC 8 cut(s) 492, 723, 1451, 1820, 2594, 4087, 4765, 4878
NruI TCGCGA 3 cut(s) 1733, 2756, 4257
NsiI ATGCAT 4 cut(s) 385, 2208, 2985, 5780
NspI RCATGY 5 cut(s) 2983, 3915, 5546, 7085, 7189
NspV TTCGAA 4 cut(s) 374, 6778, 7168, 7543
OliI CACNNNNGTG 2 cut(s) 7481, 7661
PaeI GCATGC 2 cut(s) 7085, 7189
PaeR7I CTCGAG 2 cut(s) 4278, 5882
PagI TCATGA 5 cut(s) 243, 2236, 3322, 3421, 3669
PasI CCCWGGG 2 cut(s) 5218, 6955
PceI AGGCCT 2 cut(s) 2501, 7430
PciI ACATGT 1 cut(s) 5542
PciSI GCTCTTC 3 cut(s) 3423, 7085, 7543
PctI GAATGC 5 cut(s) 385, 1053, 1060, 4163, 5909
PdiI GCCGGC 1 cut(s) 1255
PdmI GAANNNNTTC 7 cut(s) 1724, 2517, 5202, 6337, 6510, 6746, 7725
PflFI GACNNNGTC 1 cut(s) 4241
PflMI CCANNNNNTGG 4 cut(s) 166, 5236, 6223, 6998
PfoI TCCNGGA 1 cut(s) 795
PinAI ACCGGT 1 cut(s) 6792
PleI GAGTC 9 cut(s) 25, 63, 2094, 3006, 3149, 3162, 5823, 6274, 6911
PluTI GGCGCC 1 cut(s) 5653
PmaCI CACGTG 1 cut(s) 7696
PmlI CACGTG 1 cut(s) 7696
PpsI GAGTC 9 cut(s) 25, 63, 2094, 3006, 3149, 3162, 5823, 6274, 6911
Ppu21I YACGTR 4 cut(s) 6536, 7043, 7412, 7696
PpuMI RGGWCCY 1 cut(s) 3580
PscI ACATGT 1 cut(s) 5542
PshAI GACNNNNGTC 1 cut(s) 906
PshBI ATTAAT 1 cut(s) 6833
PsiI TTATAA 1 cut(s) 6032
Psp124BI GAGCTC 2 cut(s) 440, 7385
Psp1406I AACGTT 1 cut(s) 3699
Psp5II RGGWCCY 1 cut(s) 3580
Psp6I CCWGG 9 cut(s) 795, 852, 1613, 5217, 5652, 6464, 6601, 6954, 7020
PspCI CACGTG 1 cut(s) 7696
PspFI CCCAGC 4 cut(s) 1967, 2807, 7085, 7189
PspGI CCWGG 9 cut(s) 795, 852, 1613, 5217, 5652, 6464, 6601, 6954, 7020
PspOMI GGGCCC 1 cut(s) 3309
PspPPI RGGWCCY 1 cut(s) 3580
PspXI VCTCGAGB 1 cut(s) 4278
PsrI GAACNNNNNNTAC 2 cut(s) 4741, 4773
PstI CTGCAG 6 cut(s) 760, 2665, 2970, 3397, 7030, 7678
PstNI CAGNNNCTG 9 cut(s) 1916, 2255, 2710, 3287, 3890, 4423, 5824, 6050, 7034
PsyI GACNNNGTC 1 cut(s) 4241
PvuII CAGCTG 4 cut(s) 761, 3203, 5282, 6050
RruI TCGCGA 3 cut(s) 1733, 2756, 4257
RseI CAYNNNNRTG 9 cut(s) 894, 2162, 2958, 4156, 7155, 7184, 7337, 7481, 7661
Rsr2I CGGWCCG 1 cut(s) 5080
RsrII CGGWCCG 1 cut(s) 5080
SacI GAGCTC 2 cut(s) 440, 7385
SacII CCGCGG 1 cut(s) 1839
SapI GCTCTTC 3 cut(s) 3423, 7085, 7543
ScaI AGTACT 4 cut(s) 5147, 5422, 6510, 7208
SchI GAGTC 9 cut(s) 26, 63, 2095, 3006, 3150, 3162, 5824, 6274, 6911
SfoI GGCGCC 1 cut(s) 5651
Sfr274I CTCGAG 2 cut(s) 4278, 5882
Sfr303I CCGCGG 1 cut(s) 1839
SfuI TTCGAA 4 cut(s) 374, 6778, 7168, 7543
SgrBI CCGCGG 1 cut(s) 1839
SlaI CTCGAG 2 cut(s) 4278, 5882
SmiI ATTTAAAT 1 cut(s) 3735
SmiMI CAYNNNNRTG 9 cut(s) 894, 2162, 2958, 4156, 7155, 7184, 7337, 7481, 7661
SmlI CTYRAG 9 cut(s) 581, 937, 1259, 1586, 4278, 4337, 5882, 6626, 7376
SmoI CTYRAG 9 cut(s) 581, 937, 1259, 1586, 4278, 4337, 5882, 6626, 7376
SphI GCATGC 2 cut(s) 7085, 7189
SseBI AGGCCT 2 cut(s) 2501, 7430
SspDI GGCGCC 1 cut(s) 5649
SstI GAGCTC 2 cut(s) 440, 7385
StuI AGGCCT 2 cut(s) 2501, 7430
StyI CCWWGG 7 cut(s) 2560, 4439, 5124, 6009, 6800, 7431, 7599
SwaI ATTTAAAT 1 cut(s) 3735
TaqII GACCGA 1 cut(s) 5068
TauI GCSGC 5 cut(s) 260, 2141, 4982, 5537, 6239
TseFI GTSAC 8 cut(s) 492, 723, 1451, 1820, 2594, 4087, 4765, 4878
Tsp45I GTSAC 8 cut(s) 492, 723, 1451, 1820, 2594, 4087, 4765, 4878
Tth111I GACNNNGTC 1 cut(s) 4241
Van91I CCANNNNNTGG 4 cut(s) 166, 5236, 6223, 6998
Vha464I CTTAAG 4 cut(s) 937, 1259, 6626, 7376
VspI ATTAAT 1 cut(s) 6833
XagI CCTNNNNNAGG 5 cut(s) 1586, 4794, 4811, 6762, 7435
XapI RAATTY 9 cut(s) 109, 293, 661, 734, 2730, 2922, 4056, 4117, 7369
XbaI TCTAGA 4 cut(s) 665, 1811, 2725, 2986
XceI RCATGY 5 cut(s) 2983, 3915, 5546, 7085, 7189
XcmI CCANNNNNNNNNTGG 6 cut(s) 1040, 2167, 2715, 4573, 7186, 7480
XhoI CTCGAG 2 cut(s) 4278, 5882
XmaJI CCTAGG 1 cut(s) 4439
XmiI GTMKAC 5 cut(s) 1578, 2060, 2089, 2339, 5999
XmnI GAANNNNTTC 7 cut(s) 1724, 2517, 5202, 6337, 6510, 6746, 7725
ZraI GACGTC 2 cut(s) 123, 7344
ZrmI AGTACT 4 cut(s) 5147, 5422, 6510, 7208
Zsp2I ATGCAT 4 cut(s) 385, 2208, 2985, 5780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.