Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0004626

Physical Location & Seq

Genomic Coordinates Reverse (-)

31284737 .. 31293703

Gene Structure 8967 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1233800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3639 bp

ATGGCCTGCCTTAGTGCTGTCAAATGTATTCCTGCTGTTGCCAGTTGGTCTCTTCCTCAGAATGTTGAAGTCGCTACAGGCATTTGGGTTGCTTTACATGACCCAGAAAAGTCTGTTGCAGAAGCAGCAGAGGATTTATGGGATCGGTATGGGCATGACTTTGGGACTGACCATTCAGGCCTTTTCAAAGCACTTTCCCATATCAATTATAATGTCCATTTTGCTGCTGCGGAGGCATTAGCAGCTGCTTTGGATGAATGTCCCGATTCTATACAGGAGTCTCTTTCTACTTTATTTTCTCTGTATATCCATGATGCTGGTTTGACTGAGGAGAATGTTGATGCTGGTTGGCTGGGTCGACAAGGTGTAGCCTTGGCATTACATTCTTCTGCTGATGTACTCAGAACAAAAGACCTTCCTGTTGTTATGAAATTTCTTATTTCAACAGCACTGGGTGATCCTAATGCCGATGTTAGGGGAAGAATGATCAATGCTGGTTTTATGATTATTGATAAGCATGGCAGGGATAATGTTTCCTTGCTATTTCCCATCTTTGAGAACTACTTGAACAAAAAGCTTTTCATTTTTGTATTTCCTTTCCATTACAGGAATTCAGCCAAATGTCGTGAAGGAGCTTTGCTCGGATTTGAGTGTCTCTGTGAATCACTCGGAAGACTATTTGAACCGTATGTAATTAAAATGTTGCCACTACTGTTGGTTTCATTCTCGGATCAAGTTGTTGCAGTTCGTGAAGGTGCTGAGTGTTCAGCTCGTGCAATGATGTCTCAACTCAGTGCTCAAGGAGTTAAACTGGTTCTTCCATCTCTTTTGAAGGGTCTTGAAGATAAAGCTTGGCGAACAAAACAAAGTAGTGTCCTGCTGCTTGGGGCTATGGCCTATTGTGCTCCTCAACAGCTGTCTCAGTGTCTCCCGAAGATTGTTCCAAAATTGACTGAGGTTTGTTTAACAGACACACATCCTAAAGTCCAGTCAGCAGGACAAACGGCAGTTCAACAGGTTGGGAGTGTAATAAAGAATCCTGAAATAGCTTCCCTTGTTCCTACTCTTCTACTGGGTCTTTCAGATCCAAATGACCATACAAAGTACTCTCTTGATATTCTCTTACAGACCACTTTTATTAATACAATTGATGCTCCTTCACTGGCACTGTTGGTGCCAATAGTACATAGAGGGCTACGGGAAAGGAGTGCTGAGACTAAAAAGAAAGCAGCACAAATAGTTGGCAACATGTGCTCATTGGTTACTGAACCTAAGGATATGATTCCATATATTGGCTTACTTCTTCCTGAAGTGAAAAAGTTTCTTGTGGATCCAATACCAGAAGTTCCGTCAGTTGCAGCAAGGGCCCTTGGATCCCTTATAAGGGGAATGGGCGAAGATCATTTCCCAGATCTCGTTCCCTGGTTGTTTGATACACTTAAATCTGATAATAGTAATGTTGAGTGCTCTGGAGCAGCTCAAGGGTTGAGCGAGGTGTTAGCTGCACTTGGAACTGAGTACTTTGAGCTTGCACTTCCTGATATTATCCGAAACTGTTCTCATCAAAAAGCGTCTGTCCGTGATGGATATTTAACATTATTTAAGTATTTACCAAGGTCATTGGGCGTCCAATTCCAGAACTATTTACAGCAGGTCTTGCCGATGAAAATGAGTGTACGTGAGGCAGCACTAGGTGCTGGGCATATCCTGGTTGAGCATTACGCAACAACTGCCATGGGTCTTTACCTTTGCTCCTTCCTGTTGTTGAAGATGGTATCTTTAACGATAGTTGACGGATTCGGCAAAGCTCTGTGGAATTATTGGGTTGCTGGAACTTCTGGGAAAGCTTTACTTGAGGGTGGCAGTGATGATGAAGGTGCTAGTAATGAAGCACAGGGTCGGGCTATCATTGAGGTTTTGGGAAGGGAAAAGCGTGATGAAGGTCTTGCTGCATTATATATGGTTCGAACAGATGTCAACTTATCAGTTCGTCAGGCTGCTCTGCATGTATGGAAAACTATTGTGGCAAATACTCCGAAGACTCTAAAGGAAATAATGCCAGTTTTAATGAATACTCTAATTGCCTCTCTTGCTTCATCATCCTCTGAAAGGCGGCAGGTTGCTGGGCGATCACTGGGTGAGCTTGTCAGGAAACTTGGTGAAAGAGTACTTCCATGGATCATCCCAATTTTATCCCAAGGGCTAAAGGACTCTGACGTCGGCAAAAGACAAAATGCTGGAATGCAAGCAATCGATGAAATTGTTCCCACTCTTTTGCGTGCTTTGGAGGATGATCAAACTTCTGATACTGCTCTTGATGGCCTCAAACAAATCTTAAGTGTCAGGATTTCTGCTGTTTTGCCCCACATCTTACCCAAGCTGGTCCAGCTGCCACTTACGTATGGAGGGCCAATTGTCATACCTGGGTTTTGTTTACCAAAAGCACTTCAACCATTGCTTCCAATATTTCACCAAGGTTTAACAAGTGGATCTGCTGAACTAAGAGAGCAAGCAGCATTGGGTCTCGGTGAACTGATCGAAGTGACTAGTGAACAGGCACTGAAAGAGTTTGTCGTCCCAATCACAGGGCCTCTTATCCAGATAATAGGTGACCGGTTCCCTTGGCAAGTGAAGAGTGCTATTTTATCTACCTTGACAATCATGATAAGAAAGGGTGGAATGGTGCTGAAACCTTTTCTTCCTCAGCTTCAGACAACATTTGTAAAGTGTCTACAGGATAGCACAAGAATCGTTCGTTCAAGTGCTGCCCTTGCACTTGGAAAGCTTAGTGCTCTCAGTACCCGGGTCGACCCTTTAGTTGGAGATGTCCTATCCAGTTTGCAGGCTCTGGATAGTGGGGTTCGGGAGGCAAGTTTGTCTGCTCTTGAAGGTGTCCTAAAGCATGCTGGAAAAAGTGTGAGCAGTGCTGTTAGAACTCGTCTCTACTTGCAACTTAAGGACTTGATTCATCATGAAGATGACCAGGTTCGGATATCTGCTGCAAGCATTTTGGGGATCACATCCCAGTACCTAGAAGATGATCAACTCACTGAGCTGCTTCAGGAACTCTCTGATTTTCCACCATCTCTTAGCTTGTCTGCCAGGCATGGCTATGTACTCACAATATCATCCATGCTCAGGCACAACCCTTCCACTATTTGTTTGTCTCCAGAGTTTCCGTCTATCTTGGACCGGCTTAAGGGCACCTTGATTGATGAGAAGTTCCCACTGCGTGAAACTTCAACCAAGGCATTTGGGAGACTTATGATTCATAAGCTCCGAAGTGATCCTTCTAATACGTCACTTCATTTGGAAATTATATCATCCTTAGTGTCAGCTTTGCGCAATGATTCTAGTGAAGTTAGAAGAAAGGCATTATCTGTTATAAAACGAGTTTCTAAGGAAAATGCTTCGGTTATTTTGGCTCGTATCAATATTATTGTACCGGCTCTTGCTGAATGCTTGAAAGATGGAAGTACTCCCACGAGACTCGCAGCAGAAAGATGTGCTCTGCATGCTTTCCAATTGTCAAAGGGTCCTGAAAATGTTCAAGCTGCACAAAAGTTCATCACAGGCTGGGATGCCAGACGAATGTCAAAGCTTCCCGAAAATAGGCACATACAATACTTCAAGATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009682 GO:0016020 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0050896 GO:0051704 GO:0051707 GO:0098542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CLASP_N HEAT HEAT_2

Protein Analysis

1213

Amino Acids

132.31

Weight (kDa)

7.15

Isoelectric Point (pI)

41.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HEAT_EF3_N	PF24987	204 - 336	2.4e-34	EF3 N-terminal HEAT repeat
HEAT_GCN1	PF23271	218 - 356	1.9e-07	Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_GNC1	PF24984	374 - 460	6.4e-25	Elongation factor 3/GNC1 HEAT repeat
HEAT_EZ	PF13513	875 - 929	6.9e-06	HEAT-like repeat
HEAT_MROH2B_C	PF23227	908 - 1019	2.3e-06	MROH2B-like, HEAT repeats
HEAT_GCN1_C_2	PF25801	937 - 1187	1.2e-28	Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_GCN1_C	PF25786	1035 - 1189	2.2e-68	GCN1-like, C-terminal HEAT repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004626)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G64790 AT1G64790 AT1G64790
fragaria_vesca	FvH4_6g01410 FvH4_6g01410
malus_domestica	MD04G1233800.v1.1 MD04G1233900.v1.1 MD04G1234100.v1.1 MD12G1252000.v1.1
prunus_persica	Prupe.6G353600_v2.0.a1 Prupe.6G353600_v2.0.a1 Prupe.6G353600_v2.0.a1 Prupe.6G353600_v2.0.a1 Prupe.6G353600_v2.0.a1
pyrus_communis	pycom04g20770 pycom04g20790 pycom07g20320 pycom12g23140
rosa_chinensis	RchiOBHm_Chr3g0449041 RchiOBHm_Chr6g0282721
rosa_laevigata	RLG00000025859
rosa_multiflora	Rmu_co8514751.1_g000001
rosa_roxburghii	Rroxscaffold_6G00427150 Rroxscaffold_7G00190110
rosa_rugosa	Rorug02G0617500
rosa_samantha	Rh3AG016000 Rh3BG016200 Rh3CG015200 Rh3DG016500
rosa_wichuraiana	Rw3G001200 Rw5G031850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	210, 1382, 3386
AasI	GACNNNNNNGTC	1 cut(s)	2216
AatII	GACGTC	1 cut(s)	2220
Acc16I	TGCGCA	1 cut(s)	3344
Acc36I	ACCTGC	2 cut(s)	1642, 2107
AccB1I	GGYRCC	2 cut(s)	1174, 3203
AccB7I	CCANNNNNTGG	1 cut(s)	1292
AccI	GTMKAC	3 cut(s)	358, 2731, 2808
AciI	CCGC	2 cut(s)	230, 2113
AcsI	RAATTY	2 cut(s)	431, 610
AcuI	CTGAAG	3 cut(s)	1329, 2693, 3044
AcyI	GRCGYC	2 cut(s)	1626, 2217
AdeI	CACNNNGTG	4 cut(s)	455, 1694, 2138, 3233
AflII	CTTAAG	3 cut(s)	2335, 2954, 3197
AflIII	ACRYGT	1 cut(s)	1248
AgeI	ACCGGT	1 cut(s)	2613
AhlI	ACTAGT	1 cut(s)	2546
AjnI	CCWGG	5 cut(s)	1421, 1707, 2422, 2982, 3101
Alw21I	GWGCWC	6 cut(s)	799, 907, 1256, 1469, 2794, 3511
AlwNI	CAGNNNCTG	2 cut(s)	2560, 2848
Ama87I	CYCGRG	1 cut(s)	2802
AoxI	GGCC	7 cut(s)	3, 178, 894, 1365, 2320, 2408, 2588
ApaI	GGGCCC	1 cut(s)	1369
ApoI	RAATTY	2 cut(s)	431, 610
AseI	ATTAAT	1 cut(s)	1140
AsiGI	ACCGGT	1 cut(s)	2613
Asp700I	GAANNNNTTC	3 cut(s)	1555, 2262, 3546
AspLEI	GCGC	1 cut(s)	3345
AspS9I	GGNCC	7 cut(s)	1365, 1366, 2383, 2408, 2588, 3190, 3536
AsuC2I	CCSGG	2 cut(s)	2803, 2804
AsuHPI	GGTGA	6 cut(s)	467, 2150, 2171, 2462, 2540, 2621
AsuII	TTCGAA	1 cut(s)	1966
AvaI	CYCGRG	1 cut(s)	2802
AvaII	GGWCC	3 cut(s)	2383, 3190, 3536
AxyI	CCTNAGG	1 cut(s)	1272
BaeGI	GKGCMC	2 cut(s)	1369, 3206
BamHI	GGATCC	2 cut(s)	1330, 1373
BanI	GGYRCC	2 cut(s)	1174, 3203
BanII	GRGCYC	1 cut(s)	1369
BauI	CACGAG	2 cut(s)	771, 3484
BbsI	GAAGAC	2 cut(s)	679, 2045
Bbv12I	GWGCWC	6 cut(s)	799, 907, 1256, 1469, 2794, 3511
BbvCI	CCTCAGC	1 cut(s)	2703
BccI	CCATC	7 cut(s)	557, 829, 1577, 1765, 2312, 3091, 3464
BceAI	ACGGC	1 cut(s)	1020
BcgI	CGANNNNNNTGC	2 cut(s)	2731, 2765
BciT130I	CCWGG	5 cut(s)	1423, 1709, 2424, 2984, 3103
BclI	TGATCA	3 cut(s)	486, 2293, 3040
BcnI	CCSGG	2 cut(s)	2803, 2804
BcuI	ACTAGT	1 cut(s)	2546
BfaI	CTAG	5 cut(s)	1691, 1881, 2547, 3032, 3354
BfmI	CTRYAG	2 cut(s)	75, 2732
BfrI	CTTAAG	3 cut(s)	2335, 2954, 3197
BfuAI	ACCTGC	2 cut(s)	1642, 2107
BglII	AGATCT	1 cut(s)	1411
BmcAI	AGTACT	4 cut(s)	1106, 1520, 2169, 3478
Bme1390I	CCNGG	7 cut(s)	1423, 1709, 2424, 2803, 2804, 2984, 3103
Bme18I	GGWCC	3 cut(s)	2383, 3190, 3536
BmeT110I	CYCGRG	1 cut(s)	2802
BmgT120I	GGNCC	7 cut(s)	1365, 1366, 2383, 2408, 2588, 3190, 3536
BmiI	GGNNCC	7 cut(s)	1176, 1332, 1367, 1375, 2618, 3205, 3537
BmrFI	CCNGG	7 cut(s)	1423, 1709, 2424, 2803, 2804, 2984, 3103
BmrI	ACTGGG	4 cut(s)	461, 1082, 2144, 3019
BmsI	GCATC	4 cut(s)	304, 331, 1141, 3571
BmuI	ACTGGG	4 cut(s)	461, 1082, 2144, 3019
BoxI	GACNNNNGTC	1 cut(s)	3592
BpiI	GAAGAC	2 cut(s)	679, 2045
BplI	GAGNNNNNCTC	2 cut(s)	624, 656
BpmI	CTGGAG	2 cut(s)	1491, 3153
Bpu10I	CCTNAGC	2 cut(s)	2703, 3137
Bpu14I	TTCGAA	1 cut(s)	1966
BpuEI	CTTGAG	3 cut(s)	783, 1464, 1874
BpuMI	CCSGG	2 cut(s)	2803, 2804
Bsa29I	ATCGAT	1 cut(s)	2253
BsaAI	YACGTR	2 cut(s)	1679, 2400
BsaHI	GRCGYC	2 cut(s)	1626, 2217
BsaI	GGTCTC	2 cut(s)	54, 2528
BsaWI	WCCGGW	1 cut(s)	2613
BsaXI	ACNNNNNCTCC	2 cut(s)	1736, 1766
Bse118I	RCCGGY	3 cut(s)	2613, 3192, 3445
Bse21I	CCTNAGG	1 cut(s)	1272
Bse3DI	GCAATG	3 cut(s)	783, 2453, 3352
BseBI	CCWGG	5 cut(s)	1423, 1709, 2424, 2984, 3103
BseCI	ATCGAT	1 cut(s)	2253
BseGI	GGATG	9 cut(s)	259, 976, 2099, 2181, 2296, 3020, 3128, 3323, 3586
BseMI	GCAATG	3 cut(s)	783, 2453, 3352
BseRI	GAGGAG	2 cut(s)	344, 897
BseSI	GKGCMC	2 cut(s)	1369, 3206
BseYI	CCCAGC	4 cut(s)	352, 1697, 2123, 3576
BsgI	GTGCAG	2 cut(s)	1488, 3540
BshFI	GGCC	7 cut(s)	5, 180, 896, 1367, 2322, 2410, 2590
BshNI	GGYRCC	2 cut(s)	1174, 3203
BshTI	ACCGGT	1 cut(s)	2613
BshVI	ATCGAT	1 cut(s)	2253
BsiHKAI	GWGCWC	6 cut(s)	799, 907, 1256, 1469, 2794, 3511
BsiHKCI	CYCGRG	1 cut(s)	2802
BsiSI	CCGG	4 cut(s)	2614, 2803, 3193, 3446
BslFI	GGGAC	3 cut(s)	178, 246, 2561
BsmBI	CGTCTC	1 cut(s)	2945
BsmFI	GGGAC	3 cut(s)	178, 246, 2561
BsmI	GAATGC	2 cut(s)	2247, 3464
BsnI	GGCC	7 cut(s)	5, 180, 896, 1367, 2322, 2410, 2590
Bso31I	GGTCTC	2 cut(s)	54, 2528
BsoBI	CYCGRG	1 cut(s)	2802
Bsp119I	TTCGAA	1 cut(s)	1966
Bsp120I	GGGCCC	1 cut(s)	1365
Bsp1286I	GDGCHC	8 cut(s)	799, 907, 1256, 1369, 1469, 2794, 3206, 3511
Bsp19I	CCATGG	2 cut(s)	1734, 2174
BspACI	CCGC	2 cut(s)	230, 2113
BspANI	GGCC	7 cut(s)	5, 180, 896, 1367, 2322, 2410, 2590
BspDI	ATCGAT	1 cut(s)	2253
BspHI	TCATGA	2 cut(s)	2661, 2971
BspLI	GGNNCC	7 cut(s)	1176, 1332, 1367, 1375, 2618, 3205, 3537
BspMI	ACCTGC	2 cut(s)	1642, 2107
BspT104I	TTCGAA	1 cut(s)	1966
BspT107I	GGYRCC	2 cut(s)	1174, 3203
BspTI	CTTAAG	3 cut(s)	2335, 2954, 3197
BspTNI	GGTCTC	2 cut(s)	54, 2528
BsrDI	GCAATG	3 cut(s)	783, 2453, 3352
BsrFI	RCCGGY	3 cut(s)	2613, 3192, 3445
BssAI	RCCGGY	3 cut(s)	2613, 3192, 3445
BssNI	GRCGYC	2 cut(s)	1626, 2217
BssSI	CACGAG	2 cut(s)	771, 3484
BssT1I	CCWWGG	9 cut(s)	372, 1369, 1613, 1734, 2174, 2197, 2473, 2621, 3246
Bst2BI	CACGAG	2 cut(s)	771, 3484
Bst2UI	CCWGG	5 cut(s)	1423, 1709, 2424, 2984, 3103
Bst4CI	ACNGT	4 cut(s)	687, 714, 1170, 1556
Bst6I	CTCTTC	3 cut(s)	57, 1071, 2627
BstACI	GRCGYC	2 cut(s)	1626, 2217
BstAFI	CTTAAG	3 cut(s)	2335, 2954, 3197
BstAPI	GCANNNNNTGC	4 cut(s)	1251, 1657, 1694, 1730
BstBAI	YACGTR	2 cut(s)	1679, 2400
BstBI	TTCGAA	1 cut(s)	1966
BstC8I	GCNNGC	9 cut(s)	7, 1530, 2247, 2280, 2511, 2844, 2904, 3004, 3516
BstDSI	CCRYGG	2 cut(s)	1734, 2174
BstEII	GGTNACC	1 cut(s)	2609
BstENI	CCTNNNNNAGG	1 cut(s)	2107
BstF5I	GGATG	9 cut(s)	259, 976, 2099, 2181, 2296, 3020, 3128, 3323, 3586
BstHHI	GCGC	1 cut(s)	3345
BstNI	CCWGG	5 cut(s)	1423, 1709, 2424, 2984, 3103
BstNSI	RCATGY	4 cut(s)	1252, 2009, 2906, 3518
BstPAI	GACNNNNGTC	1 cut(s)	3592
BstPI	GGTNACC	1 cut(s)	2609
BstSCI	CCNGG	7 cut(s)	1421, 1707, 2422, 2801, 2802, 2982, 3101
BstSFI	CTRYAG	2 cut(s)	75, 2732
BstSLI	GKGCMC	2 cut(s)	1369, 3206
BstSNI	TACGTA	1 cut(s)	2400
BstV2I	GAAGAC	2 cut(s)	679, 2045
BstX2I	RGATCY	5 cut(s)	1084, 1330, 1373, 1411, 2489
BstXI	CCANNNNNNTGG	1 cut(s)	317
BstYI	RGATCY	5 cut(s)	1084, 1330, 1373, 1411, 2489
Bsu15I	ATCGAT	1 cut(s)	2253
Bsu36I	CCTNAGG	1 cut(s)	1272
BsuRI	GGCC	7 cut(s)	5, 180, 896, 1367, 2322, 2410, 2590
BsuTUI	ATCGAT	1 cut(s)	2253
BtgI	CCRYGG	2 cut(s)	1734, 2174
BtsCI	GGATG	9 cut(s)	259, 976, 2099, 2181, 2296, 3020, 3128, 3323, 3586
BtsI	GCAGTG	3 cut(s)	1870, 2929, 3227
BveI	ACCTGC	2 cut(s)	1642, 2107
Cac8I	GCNNGC	9 cut(s)	7, 1530, 2247, 2280, 2511, 2844, 2904, 3004, 3516
CaiI	CAGNNNCTG	2 cut(s)	2560, 2848
CciI	TCATGA	2 cut(s)	2661, 2971
CfoI	GCGC	1 cut(s)	3345
Cfr10I	RCCGGY	3 cut(s)	2613, 3192, 3445
Cfr13I	GGNCC	7 cut(s)	1365, 1366, 2383, 2408, 2588, 3190, 3536
Cfr9I	CCCGGG	1 cut(s)	2802
ClaI	ATCGAT	1 cut(s)	2253
CseI	GACGC	2 cut(s)	1560, 1615
CsiI	ACCWGGT	1 cut(s)	2982
CspAI	ACCGGT	1 cut(s)	2613
CspCI	CAANNNNNGTGG	2 cut(s)	696, 731
DraIII	CACNNNGTG	4 cut(s)	455, 1694, 2138, 3233
DrdI	GACNNNNNNGTC	1 cut(s)	2216
DseDI	GACNNNNNNGTC	1 cut(s)	2216
Eam1104I	CTCTTC	3 cut(s)	57, 1071, 2627
EarI	CTCTTC	3 cut(s)	57, 1071, 2627
Eco105I	TACGTA	1 cut(s)	2400
Eco130I	CCWWGG	9 cut(s)	372, 1369, 1613, 1734, 2174, 2197, 2473, 2621, 3246
Eco147I	AGGCCT	1 cut(s)	180
Eco24I	GRGCYC	1 cut(s)	1369
Eco31I	GGTCTC	2 cut(s)	54, 2528
Eco32I	GATATC	1 cut(s)	2994
Eco47I	GGWCC	3 cut(s)	2383, 3190, 3536
Eco57I	CTGAAG	3 cut(s)	1329, 2693, 3044
Eco81I	CCTNAGG	1 cut(s)	1272
Eco88I	CYCGRG	1 cut(s)	2802
Eco91I	GGTNACC	1 cut(s)	2609
EcoNI	CCTNNNNNAGG	1 cut(s)	2107
EcoO109I	RGGNCCY	4 cut(s)	1365, 1366, 2588, 3536
EcoO65I	GGTNACC	1 cut(s)	2609
EcoRI	GAATTC	1 cut(s)	610
EcoRII	CCWGG	5 cut(s)	1421, 1707, 2422, 2982, 3101
EcoRV	GATATC	1 cut(s)	2994
EcoT14I	CCWWGG	9 cut(s)	372, 1369, 1613, 1734, 2174, 2197, 2473, 2621, 3246
EcoT38I	GRGCYC	1 cut(s)	1369
ErhI	CCWWGG	9 cut(s)	372, 1369, 1613, 1734, 2174, 2197, 2473, 2621, 3246
Esp3I	CGTCTC	1 cut(s)	2945
FalI	AAGNNNNNCTT	6 cut(s)	1836, 1868, 3191, 3223, 3274, 3306
FaqI	GGGAC	3 cut(s)	178, 246, 2561
FbaI	TGATCA	3 cut(s)	486, 2293, 3040
FblI	GTMKAC	3 cut(s)	358, 2731, 2808
FokI	GGATG	9 cut(s)	266, 963, 2086, 2168, 2303, 3007, 3115, 3310, 3593
FriOI	GRGCYC	1 cut(s)	1369
FspBI	CTAG	5 cut(s)	1691, 1881, 2547, 3032, 3354
FspI	TGCGCA	1 cut(s)	3344
GlaI	GCGC	1 cut(s)	3344
GsaI	CCCAGC	4 cut(s)	356, 1701, 2127, 3580
GsuI	CTGGAG	2 cut(s)	1491, 3153
HaeIII	GGCC	7 cut(s)	5, 180, 896, 1367, 2322, 2410, 2590
HapII	CCGG	4 cut(s)	2614, 2803, 3193, 3446
HgaI	GACGC	2 cut(s)	1560, 1615
HhaI	GCGC	1 cut(s)	3345
Hin1I	GRCGYC	2 cut(s)	1626, 2217
Hin6I	GCGC	1 cut(s)	3343
HinP1I	GCGC	1 cut(s)	3343
HincII	GTYRAC	4 cut(s)	359, 1792, 1978, 2809
HindII	GTYRAC	4 cut(s)	359, 1792, 1978, 2809
HindIII	AAGCTT	5 cut(s)	575, 849, 1845, 2783, 3599
HpaII	CCGG	4 cut(s)	2614, 2803, 3193, 3446
HphI	GGTGA	6 cut(s)	467, 2150, 2171, 2462, 2540, 2621
Hpy166II	GTNNAC	9 cut(s)	359, 1676, 1792, 1978, 2435, 2531, 2552, 2732, 2809
Hpy8I	GTNNAC	9 cut(s)	359, 1676, 1792, 1978, 2435, 2531, 2552, 2732, 2809
Hpy99I	CGWCG	1 cut(s)	2222
HpyCH4III	ACNGT	4 cut(s)	687, 714, 1170, 1556
HpyCH4IV	ACGT	4 cut(s)	1678, 2217, 2399, 3299
HpySE526I	ACGT	4 cut(s)	1678, 2217, 2399, 3299
Hsp92I	GRCGYC	2 cut(s)	1626, 2217
HspAI	GCGC	1 cut(s)	3343
Ksp22I	TGATCA	3 cut(s)	486, 2293, 3040
LmnI	GCTCC	6 cut(s)	632, 910, 1159, 1472, 1757, 3282
LweI	GCATC	4 cut(s)	304, 331, 1141, 3571
MabI	ACCWGGT	1 cut(s)	2982
MaeI	CTAG	5 cut(s)	1691, 1881, 2547, 3032, 3354
MaeII	ACGT	4 cut(s)	1678, 2217, 2399, 3299
MaeIII	GTNAC	4 cut(s)	1261, 2542, 2609, 3300
MfeI	CAATTG	3 cut(s)	1146, 2412, 3524
MflI	RGATCY	5 cut(s)	1084, 1330, 1373, 1411, 2489
MhlI	GDGCHC	8 cut(s)	799, 907, 1256, 1369, 1469, 2794, 3206, 3511
MlyI	GAGTC	4 cut(s)	287, 2035, 2204, 3483
MmeI	TCCRAC	1 cut(s)	2800
MroXI	GAANNNNTTC	3 cut(s)	1555, 2262, 3546
MslI	CAYNNNNRTG	2 cut(s)	2976, 3111
MspA1I	CMGCKG	3 cut(s)	245, 916, 2389
MspCI	CTTAAG	3 cut(s)	2335, 2954, 3197
MspI	CCGG	4 cut(s)	2614, 2803, 3193, 3446
MspR9I	CCNGG	7 cut(s)	1423, 1709, 2424, 2803, 2804, 2984, 3103
MunI	CAATTG	3 cut(s)	1146, 2412, 3524
Mva1269I	GAATGC	2 cut(s)	2247, 3464
MvaI	CCWGG	5 cut(s)	1423, 1709, 2424, 2984, 3103
NciI	CCSGG	2 cut(s)	2803, 2804
NcoI	CCATGG	2 cut(s)	1734, 2174
NlaIV	GGNNCC	7 cut(s)	1176, 1332, 1367, 1375, 2618, 3205, 3537
NmuCI	GTSAC	3 cut(s)	2542, 2609, 3300
NsbI	TGCGCA	1 cut(s)	3344
NspI	RCATGY	4 cut(s)	1252, 2009, 2906, 3518
NspV	TTCGAA	1 cut(s)	1966
PaeI	GCATGC	2 cut(s)	2906, 3518
PagI	TCATGA	2 cut(s)	2661, 2971
PceI	AGGCCT	1 cut(s)	180
PciI	ACATGT	1 cut(s)	1248
PctI	GAATGC	2 cut(s)	2247, 3464
PdmI	GAANNNNTTC	3 cut(s)	1555, 2262, 3546
PfeI	GAWTC	9 cut(s)	266, 662, 1036, 1282, 1797, 2748, 2965, 3268, 3350
PflMI	CCANNNNNTGG	1 cut(s)	1292
PinAI	ACCGGT	1 cut(s)	2613
PleI	GAGTC	4 cut(s)	286, 2035, 2204, 3483
PpsI	GAGTC	4 cut(s)	286, 2035, 2204, 3483
Ppu21I	YACGTR	2 cut(s)	1679, 2400
PpuMI	RGGWCCY	1 cut(s)	3536
PscI	ACATGT	1 cut(s)	1248
PshAI	GACNNNNGTC	1 cut(s)	3592
PshBI	ATTAAT	1 cut(s)	1140
PsiI	TTATAA	3 cut(s)	210, 1382, 3386
Psp5II	RGGWCCY	1 cut(s)	3536
Psp6I	CCWGG	5 cut(s)	1421, 1707, 2422, 2982, 3101
PspEI	GGTNACC	1 cut(s)	2609
PspFI	CCCAGC	4 cut(s)	352, 1697, 2123, 3576
PspGI	CCWGG	5 cut(s)	1421, 1707, 2422, 2982, 3101
PspN4I	GGNNCC	7 cut(s)	1176, 1332, 1367, 1375, 2618, 3205, 3537
PspOMI	GGGCCC	1 cut(s)	1365
PspPI	GGNCC	7 cut(s)	1365, 1366, 2383, 2408, 2588, 3190, 3536
PspPPI	RGGWCCY	1 cut(s)	3536
PstNI	CAGNNNCTG	2 cut(s)	2560, 2848
PsuI	RGATCY	5 cut(s)	1084, 1330, 1373, 1411, 2489
PvuII	CAGCTG	3 cut(s)	245, 916, 2389
RseI	CAYNNNNRTG	2 cut(s)	2976, 3111
SalI	GTCGAC	2 cut(s)	357, 2807
Sau96I	GGNCC	7 cut(s)	1365, 1366, 2383, 2408, 2588, 3190, 3536
ScaI	AGTACT	4 cut(s)	1106, 1520, 2169, 3478
SchI	GAGTC	4 cut(s)	287, 2035, 2204, 3483
ScrFI	CCNGG	7 cut(s)	1423, 1709, 2424, 2803, 2804, 2984, 3103
SduI	GDGCHC	8 cut(s)	799, 907, 1256, 1369, 1469, 2794, 3206, 3511
SexAI	ACCWGGT	1 cut(s)	2982
SfaNI	GCATC	4 cut(s)	304, 331, 1141, 3571
SfcI	CTRYAG	2 cut(s)	75, 2732
SfuI	TTCGAA	1 cut(s)	1966
SinI	GGWCC	3 cut(s)	2383, 3190, 3536
SmaI	CCCGGG	1 cut(s)	2804
SmiMI	CAYNNNNRTG	2 cut(s)	2976, 3111
SmlI	CTYRAG	6 cut(s)	798, 1479, 1853, 2335, 2954, 3197
SmoI	CTYRAG	6 cut(s)	798, 1479, 1853, 2335, 2954, 3197
SnaBI	TACGTA	1 cut(s)	2400
SpeI	ACTAGT	1 cut(s)	2546
SphI	GCATGC	2 cut(s)	2906, 3518
SseBI	AGGCCT	1 cut(s)	180
SsiI	CCGC	2 cut(s)	230, 2113
SspI	AATATT	2 cut(s)	2466, 3436
SspMI	CTAG	5 cut(s)	1691, 1881, 2547, 3032, 3354
StuI	AGGCCT	1 cut(s)	180
StyD4I	CCNGG	7 cut(s)	1421, 1707, 2422, 2801, 2802, 2982, 3101
StyI	CCWWGG	9 cut(s)	372, 1369, 1613, 1734, 2174, 2197, 2473, 2621, 3246
TaaI	ACNGT	4 cut(s)	687, 714, 1170, 1556
TaiI	ACGT	4 cut(s)	1681, 2220, 2402, 3302
TaqI	TCGA	5 cut(s)	358, 1966, 2253, 2538, 2808
TatI	WGTACW	7 cut(s)	397, 1104, 1183, 1518, 2167, 3115, 3476
TauI	GCSGC	1 cut(s)	2116
TfiI	GAWTC	9 cut(s)	266, 662, 1036, 1282, 1797, 2748, 2965, 3268, 3350
TseFI	GTSAC	3 cut(s)	2542, 2609, 3300
Tsp45I	GTSAC	3 cut(s)	2542, 2609, 3300
TspGWI	ACGGA	4 cut(s)	1338, 1568, 1809, 3168
TspMI	CCCGGG	1 cut(s)	2802
Van91I	CCANNNNNTGG	1 cut(s)	1292
Vha464I	CTTAAG	3 cut(s)	2335, 2954, 3197
VpaK11BI	GGWCC	3 cut(s)	2383, 3190, 3536
VspI	ATTAAT	1 cut(s)	1140
XagI	CCTNNNNNAGG	1 cut(s)	2107
XapI	RAATTY	2 cut(s)	431, 610
XceI	RCATGY	4 cut(s)	1252, 2009, 2906, 3518
XmaI	CCCGGG	1 cut(s)	2802
XmiI	GTMKAC	3 cut(s)	358, 2731, 2808
XmnI	GAANNNNTTC	3 cut(s)	1555, 2262, 3546
XspI	CTAG	5 cut(s)	1691, 1881, 2547, 3032, 3354
ZraI	GACGTC	1 cut(s)	2218
ZrmI	AGTACT	4 cut(s)	1106, 1520, 2169, 3478

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.