RLG00000025859

Translational activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0004626
Physical Location & Seq
Reverse (-)
48710892 .. 48714488
3597 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025859

Sequence Viewer

Length: 1260 bp
ATGCACAAACAGTTTGTTTTCTTTGAAAGTTTGAATAAGATAAAAAATCCTTCTATGCGCCGTGGCATCTTCGCCTTACAAGGTTTAATAAGTGGATCTGCTGAACTGAGAGAGCAAGCGGCACTGGGTCTTGGAGAACTGATTGAAGTGACTAGTGAAAAGGCACTCAAAGAGTTTGTCATTCCAATTACAGGGCCTCTTATCCGGATAATAGGTGACCGGTTCCCTTGGCAGGTGAAGAGTGCTATTTTGTCTACCTTGACAATCATAATCAGAAAAGGTGGGATGGCCTTGAAACCATTTCTTCCGCAGCTTCAAACAACATTTGTCAAGTGTCTACAGGATAACACAAGAATTGTTCGCTCAAGTGCTGCCCTTGCGCTCGGAAAGCTTAGTGCTTTAAGTACCCGGGTGGACCCTCTAGTTGGAGACCTCCTGTCCAGTCTGCAGGCTTTGGATAGTGGGGTTCGGGAGGCAAGTTTGTCTGCTTTAGAAGGTGTCCTAAAGTATGCTGGAAAGAGCATGAGCAGTGCTGTTAGAACTCGTGTCTACTTGCAGCTTAAGGATTTGATTCATCATGATGATGATGAGGTTCGAGTATCTGCTGCAAGCATTTTGGGTATCATGTCCCAGTACATTGAAGATACCCAGCTGACCGAGCTGCTTCAGGAACTATCAAGTTTTCCATCGTCGCTCAGCTGGACTGCTAGGCATGGTTCTGTACTTACAATTTCATCCATGCTCAGGCACATCCCATCCACCATCTGTACTTCTCCAGTGTTTCCGTCTATCCTGGACCAGCTTAAAGCTGCCTTGACTGATGAGAAGTTCCCTCTTCGTGAAACTTCAACCAAGGCATTTGGGAGACTTCTTGTTTATAAAGTCCGAAGTGATCCTTCTAATACCTCAATACAATTAGATATTATATCGTCTTTAGTCTCAGCATTGCATGATGATTCTAGCGAAGTTAGAAGAAAGGCGTTATCTGCTCTAAAGGCAGTTTCGAAGGAAAGTCCTTCACCTATTGCGGCCCATATCAATATTATTGGACCTGCACTGGCTGAATGTTTGAAGGATGGAAGTACACCCGTGAGACTTGCAGCAGAAAGATGTGCTCTGCATGCCTTCCAATTGACAAAGGGTCCTGAAAATGTTCAAGCTGCACAAAAGTTCATCACAGGCTTGGAAGCTAGACGAATATCAAAGTTTTCAGAACATAGTGATGATAGTGAAGGTGAGGATGACTTGGCAAGTGGCTGA

Protein Analysis

420

Amino Acids

45.53

Weight (kDa)

8.56

Isoelectric Point (pI)

52.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEAT_EZ PF13513 77 - 131 5e-06 HEAT-like repeat
HEAT_MROH2B_C PF23227 110 - 224 3.8e-08 MROH2B-like, HEAT repeats
HEAT_GCN1_C_2 PF25801 139 - 390 1.1e-33 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_GCN1_C PF25786 237 - 391 5.2e-76 GCN1-like, C-terminal HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 879
AarI CACCTGC 1 cut(s) 223
Acc36I ACCTGC 2 cut(s) 223, 1060
AccI GTMKAC 3 cut(s) 254, 337, 549
AccIII TCCGGA 1 cut(s) 204
AciI CCGC 3 cut(s) 119, 308, 1028
AclWI GGATC 2 cut(s) 103, 887
AcuI CTGAAG 1 cut(s) 650
AfaI GTAC 5 cut(s) 406, 635, 723, 769, 1084
AfiI CCNNNNNNNGG 4 cut(s) 191, 232, 425, 744
AflII CTTAAG 1 cut(s) 560
AgeI ACCGGT 1 cut(s) 219
AgsI TTSAA 9 cut(s) 26, 34, 146, 295, 317, 641, 849, 1072, 1157
AhdI GACNNNNNGTC 2 cut(s) 436, 1140
AhlI ACTAGT 1 cut(s) 152
AjnI CCWGG 1 cut(s) 792
Alw21I GWGCWC 1 cut(s) 1117
Alw26I GTCTC 4 cut(s) 423, 859, 943, 1087
AlwI GGATC 2 cut(s) 103, 887
Ama87I CYCGRG 1 cut(s) 408
Aor13HI TCCGGA 1 cut(s) 204
AoxI GGCC 3 cut(s) 194, 288, 1029
ApeKI GCWGC 8 cut(s) 310, 371, 556, 605, 661, 809, 1100, 1160
ArsI GACNNNNNNTTYG 2 cut(s) 162, 194
AsiGI ACCGGT 1 cut(s) 219
Asp700I GAANNNNTTC 1 cut(s) 1152
AspLEI GCGC 2 cut(s) 60, 382
AspS9I GGNCC 6 cut(s) 194, 415, 796, 1030, 1049, 1142
AsuC2I CCSGG 2 cut(s) 409, 410
AsuHPI GGTGA 4 cut(s) 227, 247, 1011, 1247
AsuII TTCGAA 1 cut(s) 1004
AvaI CYCGRG 1 cut(s) 408
AvaII GGWCC 4 cut(s) 415, 796, 1049, 1142
BauI CACGAG 1 cut(s) 543
Bbv12I GWGCWC 1 cut(s) 1117
BbvI GCAGC 8 cut(s) 322, 358, 568, 592, 648, 796, 1112, 1147
BccI CCATC 5 cut(s) 280, 694, 763, 770, 1070
BceAI ACGGC 1 cut(s) 45
BciT130I CCWGG 1 cut(s) 794
BcnI CCSGG 2 cut(s) 409, 410
BcoDI GTCTC 4 cut(s) 423, 859, 943, 1087
BcuI ACTAGT 1 cut(s) 152
BfaI CTAG 5 cut(s) 153, 422, 708, 960, 1191
BfmI CTRYAG 2 cut(s) 338, 446
BfrI CTTAAG 1 cut(s) 560
BfuAI ACCTGC 2 cut(s) 223, 1060
BlpI GCTNAGC 1 cut(s) 695
Bme1390I CCNGG 3 cut(s) 409, 410, 794
Bme18I GGWCC 4 cut(s) 415, 796, 1049, 1142
BmeRI GACNNNNNGTC 2 cut(s) 436, 1140
BmeT110I CYCGRG 1 cut(s) 408
BmgT120I GGNCC 6 cut(s) 194, 415, 796, 1030, 1049, 1142
BmiI GGNNCC 3 cut(s) 224, 417, 1143
BmrFI CCNGG 3 cut(s) 409, 410, 794
BmrI ACTGGG 2 cut(s) 134, 625
BmsI GCATC 1 cut(s) 75
BmuI ACTGGG 2 cut(s) 134, 625
BpmI CTGGAG 1 cut(s) 759
Bpu10I CCTNAGC 1 cut(s) 743
Bpu1102I GCTNAGC 1 cut(s) 695
Bpu14I TTCGAA 1 cut(s) 1004
BpuEI CTTGAG 1 cut(s) 349
BpuMI CCSGG 2 cut(s) 409, 410
BsaI GGTCTC 1 cut(s) 423
BsaJI CCNNGG 4 cut(s) 61, 227, 408, 852
BsaWI WCCGGW 2 cut(s) 204, 219
Bsc4I CCNNNNNNNGG 4 cut(s) 191, 232, 425, 744
Bse118I RCCGGY 1 cut(s) 219
Bse1I ACTGG 5 cut(s) 129, 441, 631, 776, 1062
Bse3DI GCAATG 1 cut(s) 944
BseAI TCCGGA 1 cut(s) 204
BseBI CCWGG 1 cut(s) 794
BseDI CCNNGG 4 cut(s) 61, 227, 408, 852
BseGI GGATG 6 cut(s) 291, 734, 750, 755, 1081, 1246
BseLI CCNNNNNNNGG 4 cut(s) 191, 232, 425, 744
BseMI GCAATG 1 cut(s) 944
BseMII CTCAG 4 cut(s) 98, 709, 757, 954
BseNI ACTGG 5 cut(s) 129, 441, 631, 776, 1062
BseXI GCAGC 8 cut(s) 322, 358, 568, 592, 648, 796, 1112, 1147
BseYI CCCAGC 1 cut(s) 648
BsgI GTGCAG 2 cut(s) 1038, 1146
BshFI GGCC 3 cut(s) 196, 290, 1031
BshTI ACCGGT 1 cut(s) 219
BsiHKAI GWGCWC 1 cut(s) 1117
BsiHKCI CYCGRG 1 cut(s) 408
BsiSI CCGG 3 cut(s) 205, 220, 409
BslFI GGGAC 1 cut(s) 613
BslI CCNNNNNNNGG 4 cut(s) 191, 232, 425, 744
BsmAI GTCTC 4 cut(s) 423, 859, 943, 1087
BsmFI GGGAC 1 cut(s) 613
BsnI GGCC 3 cut(s) 196, 290, 1031
Bso31I GGTCTC 1 cut(s) 423
BsoBI CYCGRG 1 cut(s) 408
Bsp119I TTCGAA 1 cut(s) 1004
Bsp1286I GDGCHC 1 cut(s) 1117
Bsp13I TCCGGA 1 cut(s) 204
Bsp143I GATC 2 cut(s) 95, 892
Bsp1720I GCTNAGC 1 cut(s) 695
BspACI CCGC 3 cut(s) 119, 308, 1028
BspANI GGCC 3 cut(s) 196, 290, 1031
BspCNI CTCAG 4 cut(s) 99, 708, 756, 953
BspEI TCCGGA 1 cut(s) 204
BspHI TCATGA 1 cut(s) 577
BspLI GGNNCC 3 cut(s) 224, 417, 1143
BspMAI CTGCAG 1 cut(s) 450
BspMI ACCTGC 2 cut(s) 223, 1060
BspPI GGATC 2 cut(s) 103, 887
BspT104I TTCGAA 1 cut(s) 1004
BspTI CTTAAG 1 cut(s) 560
BspTNI GGTCTC 1 cut(s) 423
BsrDI GCAATG 1 cut(s) 944
BsrFI RCCGGY 1 cut(s) 219
BsrI ACTGG 5 cut(s) 129, 441, 631, 776, 1062
BssAI RCCGGY 1 cut(s) 219
BssECI CCNNGG 4 cut(s) 61, 227, 408, 852
BssMI GATC 2 cut(s) 95, 892
BssSI CACGAG 1 cut(s) 543
BssT1I CCWWGG 2 cut(s) 227, 852
Bst2BI CACGAG 1 cut(s) 543
Bst2UI CCWGG 1 cut(s) 794
Bst4CI ACNGT 1 cut(s) 12
Bst6I CTCTTC 2 cut(s) 233, 840
BstAFI CTTAAG 1 cut(s) 560
BstBI TTCGAA 1 cut(s) 1004
BstC8I GCNNGC 4 cut(s) 117, 450, 610, 1122
BstDEI CTNAG 5 cut(s) 107, 392, 695, 743, 940
BstDSI CCRYGG 1 cut(s) 61
BstEII GGTNACC 1 cut(s) 215
BstF5I GGATG 6 cut(s) 291, 734, 750, 755, 1081, 1246
BstHHI GCGC 2 cut(s) 60, 382
BstKTI GATC 2 cut(s) 98, 895
BstMAI GTCTC 4 cut(s) 423, 859, 943, 1087
BstMBI GATC 2 cut(s) 95, 892
BstMWI GCNNNNNNNGC 6 cut(s) 377, 388, 658, 986, 995, 1121
BstNI CCWGG 1 cut(s) 794
BstNSI RCATGY 1 cut(s) 1124
BstPI GGTNACC 1 cut(s) 215
BstSCI CCNGG 3 cut(s) 407, 408, 792
BstSFI CTRYAG 2 cut(s) 338, 446
BstV1I GCAGC 8 cut(s) 322, 358, 568, 592, 648, 796, 1112, 1147
BstX2I RGATCY 1 cut(s) 95
BstYI RGATCY 1 cut(s) 95
BsuRI GGCC 3 cut(s) 196, 290, 1031
BtgI CCRYGG 1 cut(s) 61
BtsCI GGATG 6 cut(s) 291, 734, 750, 755, 1081, 1246
BtsI GCAGTG 1 cut(s) 535
BtsIMutI CAGTG 4 cut(s) 122, 535, 783, 1055
BveI ACCTGC 2 cut(s) 223, 1060
Cac8I GCNNGC 4 cut(s) 117, 450, 610, 1122
CciI TCATGA 1 cut(s) 577
CfoI GCGC 2 cut(s) 60, 382
Cfr10I RCCGGY 1 cut(s) 219
Cfr13I GGNCC 6 cut(s) 194, 415, 796, 1030, 1049, 1142
Cfr9I CCCGGG 1 cut(s) 408
Csp6I GTAC 5 cut(s) 405, 634, 722, 768, 1083
CspAI ACCGGT 1 cut(s) 219
CviAII CATG 7 cut(s) 523, 578, 625, 713, 739, 950, 1121
CviQI GTAC 5 cut(s) 405, 634, 722, 768, 1083
DdeI CTNAG 5 cut(s) 107, 392, 695, 743, 940
DpnI GATC 2 cut(s) 97, 894
DpnII GATC 2 cut(s) 95, 892
DriI GACNNNNNGTC 2 cut(s) 436, 1140
Eam1104I CTCTTC 2 cut(s) 233, 840
Eam1105I GACNNNNNGTC 2 cut(s) 436, 1140
EarI CTCTTC 2 cut(s) 233, 840
Eco130I CCWWGG 2 cut(s) 227, 852
Eco31I GGTCTC 1 cut(s) 423
Eco47I GGWCC 4 cut(s) 415, 796, 1049, 1142
Eco57I CTGAAG 1 cut(s) 650
Eco88I CYCGRG 1 cut(s) 408
Eco91I GGTNACC 1 cut(s) 215
EcoO109I RGGNCCY 2 cut(s) 194, 1142
EcoO65I GGTNACC 1 cut(s) 215
EcoRII CCWGG 1 cut(s) 792
EcoT14I CCWWGG 2 cut(s) 227, 852
ErhI CCWWGG 2 cut(s) 227, 852
FaeI CATG 7 cut(s) 526, 581, 628, 716, 742, 953, 1124
FalI AAGNNNNNCTT 2 cut(s) 880, 912
FaqI GGGAC 1 cut(s) 613
FatI CATG 7 cut(s) 522, 577, 624, 712, 738, 949, 1120
FblI GTMKAC 3 cut(s) 254, 337, 549
FokI GGATG 6 cut(s) 298, 721, 737, 742, 1088, 1253
FspBI CTAG 5 cut(s) 153, 422, 708, 960, 1191
GlaI GCGC 2 cut(s) 59, 381
GsaI CCCAGC 1 cut(s) 652
GsuI CTGGAG 1 cut(s) 759
HaeIII GGCC 3 cut(s) 196, 290, 1031
HapII CCGG 3 cut(s) 205, 220, 409
HhaI GCGC 2 cut(s) 60, 382
Hin1II CATG 7 cut(s) 526, 581, 628, 716, 742, 953, 1124
Hin6I GCGC 2 cut(s) 58, 380
HinP1I GCGC 2 cut(s) 58, 380
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 2 cut(s) 571, 956
HpaII CCGG 3 cut(s) 205, 220, 409
HphI GGTGA 4 cut(s) 227, 247, 1011, 1247
Hpy166II GTNNAC 5 cut(s) 255, 338, 415, 550, 1085
Hpy188I TCNGA 4 cut(s) 275, 386, 887, 1213
Hpy188III TCNNGA 6 cut(s) 205, 470, 578, 668, 839, 1145
Hpy8I GTNNAC 5 cut(s) 255, 338, 415, 550, 1085
Hpy99I CGWCG 1 cut(s) 694
HpyAV CCTTC 8 cut(s) 60, 488, 906, 1000, 1026, 1066, 1135, 1226
HpyCH4III ACNGT 1 cut(s) 12
HpyCH4V TGCA 9 cut(s) 4, 448, 556, 608, 949, 1055, 1100, 1120, 1163
HpyF10VI GCNNNNNNNGC 6 cut(s) 377, 388, 658, 986, 995, 1121
HpyF3I CTNAG 5 cut(s) 107, 392, 695, 743, 940
Hsp92II CATG 7 cut(s) 526, 581, 628, 716, 742, 953, 1124
HspAI GCGC 2 cut(s) 58, 380
Kpn2I TCCGGA 1 cut(s) 204
Kzo9I GATC 2 cut(s) 95, 892
Lsp1109I GCAGC 8 cut(s) 322, 358, 568, 592, 648, 796, 1112, 1147
LweI GCATC 1 cut(s) 75
MaeI CTAG 5 cut(s) 153, 422, 708, 960, 1191
MaeIII GTNAC 2 cut(s) 148, 215
MalI GATC 2 cut(s) 97, 894
MboI GATC 2 cut(s) 95, 892
MboII GAAGA 6 cut(s) 61, 250, 296, 653, 827, 984
MfeI CAATTG 1 cut(s) 1130
MflI RGATCY 1 cut(s) 95
MhlI GDGCHC 1 cut(s) 1117
MluCI AATT 5 cut(s) 186, 354, 729, 914, 1130
MmeI TCCRAC 1 cut(s) 406
MnlI CCTC 8 cut(s) 207, 429, 443, 466, 583, 843, 916, 1231
MroI TCCGGA 1 cut(s) 204
MroXI GAANNNNTTC 1 cut(s) 1152
MseI TTAA 4 cut(s) 86, 401, 561, 804
MslI CAYNNNNRTG 3 cut(s) 579, 582, 1221
MspA1I CMGCKG 2 cut(s) 652, 699
MspCI CTTAAG 1 cut(s) 560
MspI CCGG 3 cut(s) 205, 220, 409
MspR9I CCNGG 3 cut(s) 409, 410, 794
MunI CAATTG 1 cut(s) 1130
MvaI CCWGG 1 cut(s) 794
MwoI GCNNNNNNNGC 6 cut(s) 377, 388, 658, 986, 995, 1121
NciI CCSGG 2 cut(s) 409, 410
NdeII GATC 2 cut(s) 95, 892
NlaIII CATG 7 cut(s) 526, 581, 628, 716, 742, 953, 1124
NlaIV GGNNCC 3 cut(s) 224, 417, 1143
NmuCI GTSAC 2 cut(s) 148, 215
NspI RCATGY 1 cut(s) 1124
NspV TTCGAA 1 cut(s) 1004
PaeI GCATGC 1 cut(s) 1124
PagI TCATGA 1 cut(s) 577
PaqCI CACCTGC 1 cut(s) 223
PdmI GAANNNNTTC 1 cut(s) 1152
PfeI GAWTC 2 cut(s) 571, 956
PfoI TCCNGGA 1 cut(s) 792
PinAI ACCGGT 1 cut(s) 219
PpuMI RGGWCCY 1 cut(s) 1142
PsiI TTATAA 1 cut(s) 879
Psp5II RGGWCCY 1 cut(s) 1142
Psp6I CCWGG 1 cut(s) 792
PspEI GGTNACC 1 cut(s) 215
PspFI CCCAGC 1 cut(s) 648
PspGI CCWGG 1 cut(s) 792
PspN4I GGNNCC 3 cut(s) 224, 417, 1143
PspPI GGNCC 6 cut(s) 194, 415, 796, 1030, 1049, 1142
PspPPI RGGWCCY 1 cut(s) 1142
PstI CTGCAG 1 cut(s) 450
PsuI RGATCY 1 cut(s) 95
PvuII CAGCTG 2 cut(s) 652, 699
RsaI GTAC 5 cut(s) 406, 635, 723, 769, 1084
RsaNI GTAC 5 cut(s) 405, 634, 722, 768, 1083
RseI CAYNNNNRTG 3 cut(s) 579, 582, 1221
SaqAI TTAA 4 cut(s) 86, 401, 561, 804
Sau3AI GATC 2 cut(s) 95, 892
Sau96I GGNCC 6 cut(s) 194, 415, 796, 1030, 1049, 1142
ScrFI CCNGG 3 cut(s) 409, 410, 794
SduI GDGCHC 1 cut(s) 1117
SfaNI GCATC 1 cut(s) 75
SfcI CTRYAG 2 cut(s) 338, 446
SfuI TTCGAA 1 cut(s) 1004
SinI GGWCC 4 cut(s) 415, 796, 1049, 1142
SmaI CCCGGG 1 cut(s) 410
SmiMI CAYNNNNRTG 3 cut(s) 579, 582, 1221
SmlI CTYRAG 2 cut(s) 364, 560
SmoI CTYRAG 2 cut(s) 364, 560
SpeI ACTAGT 1 cut(s) 152
SphI GCATGC 1 cut(s) 1124
Sse9I AATT 5 cut(s) 186, 354, 729, 914, 1130
SsiI CCGC 3 cut(s) 119, 308, 1028
SspI AATATT 1 cut(s) 1042
SspMI CTAG 5 cut(s) 153, 422, 708, 960, 1191
StyD4I CCNGG 3 cut(s) 407, 408, 792
StyI CCWWGG 2 cut(s) 227, 852
TaaI ACNGT 1 cut(s) 12
TaqI TCGA 2 cut(s) 595, 1004
TaqII GACCGA 1 cut(s) 671
TasI AATT 5 cut(s) 186, 354, 729, 914, 1130
TatI WGTACW 4 cut(s) 633, 721, 767, 1082
TauI GCSGC 2 cut(s) 122, 1031
TfiI GAWTC 2 cut(s) 571, 956
Tru1I TTAA 4 cut(s) 86, 401, 561, 804
Tru9I TTAA 4 cut(s) 86, 401, 561, 804
TscAI CASTG 4 cut(s) 129, 535, 783, 1062
TseFI GTSAC 2 cut(s) 148, 215
TseI GCWGC 8 cut(s) 310, 371, 556, 605, 661, 809, 1100, 1160
Tsp45I GTSAC 2 cut(s) 148, 215
TspDTI ATGAA 3 cut(s) 563, 723, 1162
TspGWI ACGGA 1 cut(s) 774
TspMI CCCGGG 1 cut(s) 408
TspRI CASTG 4 cut(s) 129, 535, 783, 1062
Vha464I CTTAAG 1 cut(s) 560
VpaK11BI GGWCC 4 cut(s) 415, 796, 1049, 1142
XceI RCATGY 1 cut(s) 1124
XmaI CCCGGG 1 cut(s) 408
XmiI GTMKAC 3 cut(s) 254, 337, 549
XmnI GAANNNNTTC 1 cut(s) 1152
XspI CTAG 5 cut(s) 153, 422, 708, 960, 1191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.