FvH4_6g01410

Translational activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004626
Physical Location & Seq
Forward (+)
782254 .. 803467
21214 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g01410.t1

Sequence Viewer

Length: 7863 bp
ATGGCGACTCCGACAGAGTCCCTCCTCTCCGTCGCCGGGTCGCTCTCCACTGCCTCCACTCAGAAGCGGGTTCGGATTTTCCGAGATGAAATTCCGGCCATTATCAACGGCTCCGAAATATGTGCGGAGTCTGCATCACTGTTGGTAGATATAATATTTAAAACATTATACATCTATGATGATCGTGGGTCCAAGAAAGCTGTCGATACCATCATCACAAAATGTTTTCAAGAGGTTGCCTTCATGAAGAATTTTGCAGCAGTCCTTGTTCAGAACATGGAAAAAAATGTCAGGGTGCAATCACATGTTGGCGGCCACAGACTTCTCCAGTGGTCATGTCTTCTTCTCAGCAAGAGCAAGTTCACCACAGTTTCCAAGAATGCATTCTCTAGAGTTGCCACAGTGCAGGCATCATTACTTCATGTTGTTGTACAAAGATCTCTCAGTGAGCAACAGTCTTGCAAGAAGACATTTTATCACTTATTTTCTCAGTTGCCGGAAATCAACAAATTGTATATGGAGGAGTTGAAGGAAGCACGCATCCCATACAAAGATAGTCCTGAATTATTATTGTTCTTGATGGAATTCTCAAGTACCTCAAGAAAATCATCTTCTTTGTTTGAACAATGCAAGCCAACATTTCTTGATATGTATCTCAAGGCAGTTTTAAATGCAAGAGAAAAGCCGGCAATGGGTCTTAGTGAAGCTTTTCGTCCACTTTTTCGACATATGTCGCATGAAGATTTTCAAAACATTGTGCTTCCATCTTCTGTTAAAATGCTGAAACGCAACCCTGAGATCGTATTGGAATCAGTTGGGATTCTTTTGAAGTCAATCAACCTTGATCTGAGCAAGTATGCTGTCGAAATTCTTTCACTGGTCTTGCCACAGGCTCGGCATGCAGATGAAGGCAGGAGGCTTCCTGCACTGGAAATTGTACGATGTCTCAGCCAAAATTCTAGTAATCCAGACGCTATAGAAGCAATGTTCAATGCTATCAAATCTGTCATCGGAGGCTCAGAGGGAAGACTTGCATTTCCTTACCAGAGAATTGGCATGATCACTGCACTGCAAGAATTGTGCAATTCCCCAGATGGGAAGCACCTTAATAGACTGTCACAAACTTCATGCAGTTACCTTCGATCTTGTTACAAGGAGGATGGCAATGAGGAGGTCAAGCTTGCAATTTTGTCTGCCCTAGGTTCATGGGCAGCAAGATCTGCTGATGTTGTTCAGTCAGATTTAGTTTCTTTTTTGTCTTCTGGGCTTAAGGAGAAAGAAGCTCTAAGAAGAGGGCATCTTCGTTGTCTACGAGCCATATGCAGAAATACAGATGCTGTTTATCGGTTATCATCATTACTGGAACCTCTCATTCAACTTGTCAAAACTGGTTTCACTAAAGTAGTTCAGCGTTTGGATGGTATATATGCCTTGCTATTAGTTGGCAAAATTGCAGCAGTTGACATCAAAGCAGAGGAGATCGTGGTGAGGGAAAAGATCTGGTCTTTTGTTTCTCAAAATGAACCATCTCTTCTTCCAATATCTCTGGTTTCAAAATTGGTGACTGAGGATTGCATGGCATGTGTCGATCTTCTTGAAGTCTTGCTGGTGGAACATTTGCAGAGGGCGGTGGATTCCTTTTCTGTCAGATCATTGTCACAGTTGATAATCTTTTTCATGTGCCACCCATGTTGGGATATTCGAAGAGTGGCTTATAATGCAACTAAAAAGATAGTTCCTGCTGCCCCGCAATTAGCTGAACATCTTTTGATTGAATTTGCGACTTTCATGTCTGTAGTTGAAGAGAAACATCGTATTTCAAAGTTAAGTGATACAGATAATTCAGCGGACTCCCAAGTACCGTTCCTTCCCTCGGTTGAGGTTTCGGTTAAGGCTTTACTTGTTATTTCATCAGTAGCTCTACCTGCAGCTCCAAGTGCATCAATGCGAGTTTTATTCTGTGGACATCATCCATACTTGGTTGGAACTGCAAAAAGAGATGCAGTTTGGAGGAGGCTACATAAATGTCTGCATAAATGTGGTTTTGACATAAACAGCAATATTTTGGCTGACATCAAAAATTTATGCAAGGGCCTACTGGAAACAATGTGGTTGTCAAGTACTAGTGCATCAGAGCAGCAAGCAGCCATATCTTCATTATCCACTCTGATGTCCATTGCACCTGGAGAAACGTATACGGAGTTTGAAAAGCATTTGAAACACCTTCCTTACCGATATTCACATGATACACTTTCCGAAAATGATATTCGGATATTTCATACTCCTGAAGGCATGCTTTCCAGTGAGCAAGGTGTCTACATAGCCGAGTCTGTTGCTGCCAAGAACATGAAACAAGCGAAAGGCCGTTTTCGGATGTACGAGGACCTAAATGACATGGACAATGGCAGTTCTAATCATTCTGCAAAAGTCGAGCAAAGCAGTAAAACTGGGAAGTCGACAAAGAAACCTGAGAAAGCAAAGACTGCTAAAGAGGAGGCACGAGAGTTACAACTTAAGGAGGAGGCATCCATACGTGAGAAGGTCCGAGAAATACAGAAAAATCTGTCTTTAATGCTTAAAGCTCTTGGGGAAATGGCCATTGCAAATCCTGTTTTTGCACATAGTCAACTTCGTTCCCTGGTTAACTATGTCGAGCCATTACTGAGGTCGTCAATAGTCAGCGATATCGCATATGAAACTATGGTGAAACTCTCTAGATGTACTGCTCCCCCTCTTTGTAATTGGGCCCTTGACATTGCTACTGCTTTGCGCTTAGTTGTGACTGAAGAAGATCGTCTTCTGTTGGATATGCTTTCATCAGCTGGGCAAGGGGAAGATGATAGACCATCTTTGAGTTTGTTTGAGCGAATAATTAACGCCCTTTCTGTATCTTGTAAATCGGGACCTCTTCCTGTTGATTCCTTCACTTTTGTTTTCCCCATAATGGAGCGAATTCTTTTAAGCTCTAAGAAGACCGGGCTTCATGATAAAGTGCTTCAGATCATTTATATGCACATGGACCCACTATTACCTCTCCCTAGGCTTCGAATGATTTCAGTTCTTTACCATGTTCTTGGTATTGTACAAGCTTATCAATCCTCTATTGGACCTGCGTTGAATGAGCTGTGTCTTGGTCTGCAGCCTGATGAAGTTGCACCGGCTTTATATGGAGTTTATGCAAAATATCTTCACGTTAGAATGGCCTGTCTAACTGCTGTCAAATGCATTCCTACTGTTGCCAGTCGATCTCTTATTCAGAATGTTGAGGTTGCTACGAGTATTTGGATTGCTTTGCATGACCCAGAAAAGTCAGTTGCAGAAGCTGCAGAGGATTTGTGGGATCGCTATGGACATGACTTTGGGACTGACTATTCGGGCCTTTTCAAAGCACTTTCCCATATCCATTACAATGTCCGATTTGCTGCTGCAGAGGCATTAGCTGCTGCTTTGGATGAATCTCCTGATTCTATACAGGAATCCCTTTCTACTTTATTCTCCCTGTATATCCGTGACGCTGGTTTGACTGAGAACAATGTCGATGCTGGTTGGCTGGGCAGGCAGGGAGTAGCTTTGGCATTGCATTCTTCTGCTGATGTGCTGAGAACAAAAGACCTTCCTGTTGTTATGACATTTTTGATTTCAAGAGCATTGGCAGATCCTAATGCTGATGTTCGGGGGAGAATGATTAATGCTGGCATTATGATTATTGATAAGCACGGAAAAGATAATGTGTCTTTGCTATTTCCTATCTTTGAGAACTACTTAAACAAAAAGGCATCGGATGAGGAGACATATGATTTGGTTCGTGAGGGTGTTGTTATTTTCACCGGAGCTTTGGCAAAACATTTGGCAAAGGATGATCCAAAAGTACACACTGTTATAGAGAAGTTGTTGGATGTCTTGAATACTCCATCAGAAGCTGTTCAGCGGGCAGTTTCAACATGCCTATCACCATTAATGCAATCAAAGCAAGATGATGGCCAAGCTCTTGTTTCACGGATTTTGGATCAATTGATGAACAGTGATAAGTATGGGGAACGCCGTGGAGCTGCTTTTGGGCTTGCTGGAGTAGTCAAGGGATTTGGTATTTCTTCCTTGAAGAAGTATGGAATTGTGAACATACTACAGGAAGGTCTTGTTGATAGGAACTCTGCAAAACGTCGGGAAGGGGGTTTGCTTGGATTTGAGTGTCTATGTGAAACACTTGGAAAATTATTTGAACCGTATGTAATACAAATGTTGCCACTACTGTTGGTCTCATTTTCTGATCAAGTTGTTGCAGTGCGTGAAGGTGCTGAGTGTGCTGCTCGTGCAATGATGTCTCACCTTACTGCTCAGGGAGTGAAACTGGTCCTTCCATCTCTTTTGAAGGGTCTTGAAGATAAAGCTTGGCGAACAAAGCAAAGTAGTGTTCAACTGCTTGGTGCTATGGCCTTTTGTGCTCCTCAGCAGCTGTCTCAGTGTCTGCCCAGGATTGTACCAAAACTGACTGAGGTTTTAACAGACACCCACCCTAAAGTTCAGTCGGCAGGACAAACAGCCCTTCAACAGGTTGGGAGCGTAATAAAGAATCCTGAGATAGCTTCATTGGTCCCTACTCTTCTTCTGGGTCTGACAGATCCAAATGACTATACAAAATATTCTCTGGATATTCTCTTGGGGACAACATTCATCAATTCAATTGATGCTCCTTCACTTGCACTCTTGGTGCCAATAGTACATAGAGGGCTAAGAGAGAGGGGTGCAGAGACTAAAAAGAAAGCAGCCCAAATAGTAGGCAATATGTGTTCCCTTGTTACAGAGCCAAATGATATGATTCCGTACATAGGGCTGCTTCTTCCTGAAGTGAAAAAGGTTCTTGTGGATCCAATTCCAGAAGTTAGGTCAGTTGCTGCAAGGGCCCTTGGATCCCTTATAAGGGGAATGGGTGAAGATCATTTCCCAGATCTTGTTCCCTGGTTGATGGATACACTTAAATCAGATAATAGTAATGTTGAGCGATCTGGAGCAGCTCAAGGGCTAAGTGAGGTACTAGCTGCACTTGGGACTGAGTATTTTGAGCATGTACTTCCTGATGTTATCCGAAATTGTTCTCATCAAAAAGCATCTGTGCGTGATGGGCATTTGACGTTATTCAAGTATTTACCAAGGTCCTTAGGCGTCCAATTCCAGAATTATTTACAGAAGGTGCTGCCTGCTATTATAGATGGTCTTGCTGATGAAAATGAGTCTGTACGTGAAGCAGCACTAGGTGCGGGACATGTTCTGGTTGAGCATTATGCGATAACGTCATTACCTTTGCTCCTACCTGCTGTTGAAGATGGTATCTTTAATGATAGTTGGCGAATCCGTCAAAGCTCTGTGGAATTATTGGGGGATCTGTTATTTAAGGTCGCGGGTACTTCTGGAAAAGCTTTACTGGAGGGTGGCAGTGATGATGAAGGTGCAAGTACTGAAGCACAGGGGCGGGCTATCATTGAGGTTCTTGGAAGGGATAAGCGTAATGAAATTCTTGCTGCATTGTACATGGTTCGAACTGATGTCAGCTTAACAGTTCGTCAGGCTGCGTTGCATGTATGGAAAACTATAGTGGCAAATACCCCAAAGACTCTAAAGGAAATAATGCCAGTTTTAATGAATACTCTAATTGCCTCTCTTGCATCATCATCCTCTGAAAGGCGGCAGGTTGCTGCACGTGCACTGGGTGAGCTTGTCAGGAAGCTTGGTGAGAGAGTGCTGCCATTGATCATCCCTATTTTATCCCAAGGGTTGAAAGACTCTGACACCAGTAGACGACAAGGTGTATGCATTGGCCTGAGTGAAGTAATGGCAAGTGCCGCTAAAAGTCATTTGTTGAGTTTCATGGATGAGCTAATTCCAACCATTCGAACTGCTCTTTCAGATAGCATGCCCGAAGTTCGTGAGTCTGCTGGAATAGCATTCAGCACTCTGTACAAGAATGCTGGAATGCAAGCGATTGATGAAATTGTTCCTAGTCTTTTGCATGCGTTGGAGGATGCTCGAACTTCTGATACAGCACTTGATGGTCTCAAACAAATCTTAAGTGTCAGGATTTCTGCGGTTTTACCGCACATTTTGCCCAAGCTGGTCCAGCTCCCACTGACTGCACTTAATGCACATGCTCTCGGAGCTGTAGCTGAGGTTGCCGGGCCTGGTCTTAACTCTCATCTTGGTACTGTTCTTCCTGCCTTACTTACTGCAATGGGTGATGATGCAAAGGATGTTCAAACATTGGCAAAGGAGGCTGCTGAAACTGTGGTCTTAGTTATAGATGATGAAGGTGTTGAATTTCTTACATCTGAGCTCCTTAGAGCTGTCAGCGAAAGTCAGGCTTCAATCAGAAGAAGTGCCGCTTATCTTATCGGCTATTTCTTCAAAAACAGTAAATTATATTTGGTTGATGAAGCACCAAACATGATATCTACCTTGATTGTCTTGCTCAGCGATTCTGATTCTGCTACTGTGGCAGTTTCTTGGGAAGCATTGTCAAGGGTTGTAAGTTCAGTCCCCAAGGAGGTACTTCCTTCATATATTAAGTTAGTACGAGATGCTGTGTCGACTTCCAGAGATAAGGAGCGTAGGAAAAAGAAGGGAGGACCTATTGTCATACCTGGACTTTGTTTACCAAAAGCACTTCAACCATTGCTTCCAATATTTCTCCAGGGTTTAATAAGTGGATCTGCTGAACTGAGAGAGCAAGCGGCACTGGGTCTTGGTGAACTGATTGAAGTGACTAGTGAAAAGGCACTCAAAGAGTTTGTTATTCCAATTACTGGGCCTCTTATCCGGATAATAGGTGACCGGTTCCCTTGGCAGGTGAAGAGTGCTATTTTATCTACCTTGACAATCATAATCAGAAAAGGTGGGATGGCTTTGAAACCATTTCTTCCGCAACTTCAAACAACATTTGTCAAGTGTCTACAAGATAACACAAGAGTTGTTCGCTCAAGTGCTGCGCTTGCGCTTGGAAAGCTTAGTGCTTTAAGTACCCGGGTGGACCCTCTAGTTGGAGACCTGCTGTCCAGTTTGCAGTCTTTGGATGCTGGGGTTCGGGAGGCAAGTTTGTCTGCTTTAGAAGGTGTCTTAAAGCATGCTGGGAAGAGCGTGAGCACTGCTGTTAGAACTCGTGTCTACTTACAGCTTAAGGATATGATTCATCATGATGATGACGAGGTTCGAATATCTGCTGCAAGCATTTTGGGTATCATGTCCCAGTACATTGAAGATACCCAGCTGACCGAGCTGCTTCAGGAACTGTCAAGTTTTCCATTGTCACTTAGCTGGTCTGCTCGGCATGGTTATGTACTTACAATTTCATCCATGCTCAGGCACATCCCCTCCACCGTCTGTGCTTCTACAGTGTTTCCGTCCATCCTGGACCAGCTTAAAGCAGCCTTGAAAGATGAGAAGTTCCCGCTTCGTGAAACTTCAACCAAGGCATTTGGGAGACTTCTCGTTTATAAAGTCCGAAATGATCCTTCGAATACCTCAGTACAGTTAGAAATTATATCATCTTTAGTCTCAGCATTGCATGATGATTCCAGTGAAGTTAGAAGAAAGGCGTTATCTGCTATAAAGGCAGTTTCGAAGGAAAGTTCTTCACCTATTGCAGCCCATATGAACATTATTGGACCTGCACTGGCTGAATGTTTGAAGGATGGAAGTACACCCGTGAGACTTGCAGCGGAAAGATGTGCTCTGCATGCCTTCCAATTGGCAAAGGGTCCTGATAATGTTCAAGCTGCACAGAAGTTCATCACAGGCTTAGATGCTAGACGGATATCAAAACTTTCAGAACATAGTGATGATAGTGAAGGGGAGGATGACTCGGCAAGTGGCTGA

Protein Analysis

2621

Amino Acids

286.82

Weight (kDa)

6.91

Isoelectric Point (pI)

43.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GNC1_N PF24993 211 - 344 1.8e-43 Stalled ribosome sensor GCN1 N-terminal
HEAT_EF3_N PF24987 1347 - 1514 3.8e-46 EF3 N-terminal HEAT repeat
HEAT_GCN1 PF23271 1397 - 1534 2.7e-10 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_GNC1 PF24984 1552 - 1638 1.3e-25 Elongation factor 3/GNC1 HEAT repeat
HEAT_GCN1 PF23271 1626 - 1773 3.1e-11 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_N PF24987 1667 - 1796 4.7e-08 EF3 N-terminal HEAT repeat
TPR_DNAAF5 PF25757 1836 - 1991 7.5e-07 Dynein axonemal assembly factor 5-like, TPR repeats
HEAT_GCN1 PF23271 1869 - 2017 8.1e-09 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_N PF24987 1878 - 1977 6.8e-09 EF3 N-terminal HEAT repeat
HEAT_EF3_GNC1 PF24984 1905 - 1984 6.2e-06 Elongation factor 3/GNC1 HEAT repeat
HEAT_GCN1 PF23271 2028 - 2217 1.2e-51 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_N PF24987 2044 - 2157 2.1e-06 EF3 N-terminal HEAT repeat
HEAT_MROH2B_C PF23227 2311 - 2425 8.3e-07 MROH2B-like, HEAT repeats
HEAT_GCN1_C_2 PF25801 2340 - 2591 1.9e-31 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_GCN1_C PF25786 2438 - 2592 2.4e-73 GCN1-like, C-terminal HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1716, 4916, 7482
AarI CACCTGC 1 cut(s) 6826
AasI GACNNNNNNGTC 1 cut(s) 1789
Acc36I ACCTGC 7 cut(s) 1933, 3118, 5317, 5674, 6826, 7044, 7663
AccB1I GGYRCC 1 cut(s) 4708
AccB7I CCANNNNNTGG 1 cut(s) 6794
AccI GTMKAC 8 cut(s) 1309, 2195, 2316, 2456, 5791, 6578, 6940, 7152
AccII CGCG 1 cut(s) 5396
AccIII TCCGGA 1 cut(s) 6807
AcoI YGGCCR 4 cut(s) 96, 313, 2595, 3979
AcuI CTGAAG 6 cut(s) 2307, 2805, 2981, 4863, 5475, 7253
AcvI CACGTG 1 cut(s) 5696
AcyI GRCGYC 1 cut(s) 5160
AdeI CACNNNGTG 2 cut(s) 5252, 5705
AflII CTTAAG 4 cut(s) 1268, 2513, 6061, 7163
AflIII ACRYGT 2 cut(s) 304, 5260
AgeI ACCGGT 1 cut(s) 6822
AhdI GACNNNNNGTC 2 cut(s) 6623, 7039
AhlI ACTAGT 2 cut(s) 2123, 6755
AjnI CCWGG 8 cut(s) 2182, 2637, 4469, 4955, 6172, 6631, 6681, 7395
AjuI GAANNNNNNNTTGG 2 cut(s) 368, 400
Alw21I GWGCWC 5 cut(s) 4444, 5701, 6327, 7133, 7720
Alw44I GTGCAC 1 cut(s) 5697
AlwNI CAGNNNCTG 7 cut(s) 1337, 3323, 3920, 4453, 4465, 4892, 7276
Ama87I CYCGRG 1 cut(s) 7011
Aor13HI TCCGGA 1 cut(s) 6807
ApaI GGGCCC 2 cut(s) 2749, 4903
ApaLI GTGCAC 1 cut(s) 5697
ArsI GACNNNNNNTTYG 4 cut(s) 3355, 3387, 3781, 3813
AseI ATTAAT 2 cut(s) 3687, 3956
AsiGI ACCGGT 1 cut(s) 6822
Asp700I GAANNNNTTC 7 cut(s) 383, 708, 744, 3052, 3921, 5089, 5988
AspA2I CCTAGG 2 cut(s) 1198, 3038
AspLEI GCGC 3 cut(s) 2772, 6979, 6985
AsuC2I CCSGG 5 cut(s) 37, 2977, 6169, 7012, 7013
AsuII TTCGAA 7 cut(s) 1702, 3046, 5533, 5887, 7198, 7502, 7607
AvaI CYCGRG 1 cut(s) 7011
AvrII CCTAGG 2 cut(s) 1198, 3038
AxyI CCTNAGG 1 cut(s) 5155
BaeGI GKGCMC 3 cut(s) 2749, 4903, 5701
BalI TGGCCA 2 cut(s) 2597, 3981
BamHI GGATCC 2 cut(s) 4864, 4907
BanI GGYRCC 1 cut(s) 4708
BanII GRGCYC 3 cut(s) 2749, 4903, 6327
BarI GAAGNNNNNNTAC 2 cut(s) 1851, 1883
BauI CACGAG 3 cut(s) 2499, 4308, 7146
BbrPI CACGTG 1 cut(s) 5696
BbsI GAAGAC 6 cut(s) 332, 473, 1033, 1251, 2789, 2978
Bbv12I GWGCWC 5 cut(s) 4444, 5701, 6327, 7133, 7720
BbvCI CCTCAGC 2 cut(s) 4446, 6159
BceAI ACGGC 3 cut(s) 124, 2349, 4026
BcgI CGANNNNNNTGC 6 cut(s) 104, 138, 1302, 1336, 2315, 2349
BciT130I CCWGG 8 cut(s) 2184, 2639, 4471, 4957, 6174, 6633, 6683, 7397
BciVI GTATCC 1 cut(s) 4960
BclI TGATCA 3 cut(s) 1059, 4267, 5745
BcnI CCSGG 5 cut(s) 37, 2977, 6169, 7012, 7013
BcuI ACTAGT 2 cut(s) 2123, 6755
BfrI CTTAAG 4 cut(s) 1268, 2513, 6061, 7163
BfuAI ACCTGC 7 cut(s) 1933, 3118, 5317, 5674, 6826, 7044, 7663
BfuI GTATCC 1 cut(s) 4960
BglII AGATCT 4 cut(s) 437, 1217, 1497, 4945
BlnI CCTAGG 2 cut(s) 1198, 3038
BlpI GCTNAGC 1 cut(s) 6461
BmcAI AGTACT 2 cut(s) 2122, 5452
BmeRI GACNNNNNGTC 2 cut(s) 6623, 7039
BmeT110I CYCGRG 1 cut(s) 7011
BmrI ACTGGG 5 cut(s) 2457, 5711, 6737, 6804, 7228
BmuI ACTGGG 5 cut(s) 2457, 5711, 6737, 6804, 7228
BoxI GACNNNNGTC 1 cut(s) 730
BpiI GAAGAC 6 cut(s) 332, 473, 1033, 1251, 2789, 2978
BplI GAGNNNNNCTC 1 cut(s) 7832
BpmI CTGGAG 6 cut(s) 311, 2205, 4086, 5025, 5441, 6665
Bpu10I CCTNAGC 4 cut(s) 4335, 4446, 6159, 7346
Bpu1102I GCTNAGC 1 cut(s) 6461
Bpu14I TTCGAA 7 cut(s) 1702, 3046, 5533, 5887, 7198, 7502, 7607
BpuEI CTTGAG 5 cut(s) 574, 583, 641, 4998, 6952
BpuMI CCSGG 5 cut(s) 37, 2977, 6169, 7012, 7013
BsaAI YACGTR 3 cut(s) 2534, 5237, 5696
BsaBI GATNNNNATC 1 cut(s) 651
BsaHI GRCGYC 1 cut(s) 5160
BsaI GGTCTC 3 cut(s) 4261, 6053, 7026
BsaWI WCCGGW 3 cut(s) 3827, 6807, 6822
BsaXI ACNNNNNCTCC 4 cut(s) 5286, 5316, 7343, 7373
Bse118I RCCGGY 3 cut(s) 685, 3157, 6822
Bse21I CCTNAGG 1 cut(s) 5155
Bse8I GATNNNNATC 1 cut(s) 651
BseAI TCCGGA 1 cut(s) 6807
BseBI CCWGG 8 cut(s) 2184, 2639, 4471, 4957, 6174, 6633, 6683, 7397
BseJI GATNNNNATC 1 cut(s) 651
BseRI GAGGAG 9 cut(s) 14, 536, 1184, 1490, 2026, 2507, 2534, 3800, 4434
BseSI GKGCMC 3 cut(s) 2749, 4903, 5701
BseYI CCCAGC 5 cut(s) 2822, 3550, 7064, 7115, 7251
BsgI GTGCAG 9 cut(s) 425, 909, 1050, 4764, 5022, 5676, 6111, 7641, 7749
Bsh1236I CGCG 1 cut(s) 5396
BshNI GGYRCC 1 cut(s) 4708
BshTI ACCGGT 1 cut(s) 6822
BsiHKAI GWGCWC 5 cut(s) 4444, 5701, 6327, 7133, 7720
BsiHKCI CYCGRG 1 cut(s) 7011
BslFI GGGAC 9 cut(s) 4, 2916, 3376, 4577, 4675, 5059, 5271, 6512, 7216
BsmFI GGGAC 9 cut(s) 4, 2916, 3376, 4577, 4675, 5059, 5271, 6512, 7216
BsmI GAATGC 7 cut(s) 383, 385, 3225, 3580, 5939, 5965, 5973
Bso31I GGTCTC 3 cut(s) 4261, 6053, 7026
BsoBI CYCGRG 1 cut(s) 7011
Bsp119I TTCGAA 7 cut(s) 1702, 3046, 5533, 5887, 7198, 7502, 7607
Bsp120I GGGCCC 2 cut(s) 2745, 4899
Bsp1286I GDGCHC 7 cut(s) 2749, 4444, 4903, 5701, 6327, 7133, 7720
Bsp13I TCCGGA 1 cut(s) 6807
Bsp1407I TGTACA 4 cut(s) 430, 3082, 5523, 5952
Bsp1720I GCTNAGC 1 cut(s) 6461
BspEI TCCGGA 1 cut(s) 6807
BspFNI CGCG 1 cut(s) 5396
BspHI TCATGA 3 cut(s) 243, 2983, 7180
BspMAI CTGCAG 4 cut(s) 1930, 3141, 3328, 3430
BspMI ACCTGC 7 cut(s) 1933, 3118, 5317, 5674, 6826, 7044, 7663
BspQI GCTCTTC 1 cut(s) 7115
BspT104I TTCGAA 7 cut(s) 1702, 3046, 5533, 5887, 7198, 7502, 7607
BspT107I GGYRCC 1 cut(s) 4708
BspTI CTTAAG 4 cut(s) 1268, 2513, 6061, 7163
BspTNI GGTCTC 3 cut(s) 4261, 6053, 7026
BsrFI RCCGGY 3 cut(s) 685, 3157, 6822
BsrGI TGTACA 4 cut(s) 430, 3082, 5523, 5952
BssAI RCCGGY 3 cut(s) 685, 3157, 6822
BssNAI GTATAC 1 cut(s) 2196
BssNI GRCGYC 1 cut(s) 5160
BssSI CACGAG 3 cut(s) 2499, 4308, 7146
BssT1I CCWWGG 8 cut(s) 1198, 3038, 4903, 5147, 5764, 6531, 6830, 7455
Bst1107I GTATAC 1 cut(s) 2196
Bst2BI CACGAG 3 cut(s) 2499, 4308, 7146
Bst2UI CCWGG 8 cut(s) 2184, 2639, 4471, 4957, 6174, 6633, 6683, 7397
Bst6I CTCTTC 8 cut(s) 1285, 1536, 1699, 1797, 2913, 4605, 6836, 7115
BstACI GRCGYC 1 cut(s) 5160
BstAFI CTTAAG 4 cut(s) 1268, 2513, 6061, 7163
BstAPI GCANNNNNTGC 7 cut(s) 1220, 2483, 3323, 3440, 5252, 6097, 6134
BstAUI TGTACA 4 cut(s) 430, 3082, 5523, 5952
BstBAI YACGTR 3 cut(s) 2534, 5237, 5696
BstBI TTCGAA 7 cut(s) 1702, 3046, 5533, 5887, 7198, 7502, 7607
BstDSI CCRYGG 1 cut(s) 4042
BstEII GGTNACC 1 cut(s) 6818
BstENI CCTNNNNNAGG 2 cut(s) 4547, 5674
BstFNI CGCG 1 cut(s) 5396
BstHHI GCGC 3 cut(s) 2772, 6979, 6985
BstNI CCWGG 8 cut(s) 2184, 2639, 4471, 4957, 6174, 6633, 6683, 7397
BstPAI GACNNNNGTC 1 cut(s) 730
BstPI GGTNACC 1 cut(s) 6818
BstSLI GKGCMC 3 cut(s) 2749, 4903, 5701
BstUI CGCG 1 cut(s) 5396
BstV2I GAAGAC 6 cut(s) 332, 473, 1033, 1251, 2789, 2978
BstXI CCANNNNNNTGG 2 cut(s) 1052, 3074
BstZ17I GTATAC 1 cut(s) 2196
Bsu36I CCTNAGG 1 cut(s) 5155
BsuI GTATCC 1 cut(s) 4960
BtgI CCRYGG 1 cut(s) 4042
BtsI GCAGTG 6 cut(s) 48, 1062, 1067, 4287, 5437, 7131
BveI ACCTGC 7 cut(s) 1933, 3118, 5317, 5674, 6826, 7044, 7663
CaiI CAGNNNCTG 7 cut(s) 1337, 3323, 3920, 4453, 4465, 4892, 7276
CciI TCATGA 3 cut(s) 243, 2983, 7180
CfoI GCGC 3 cut(s) 2772, 6979, 6985
Cfr10I RCCGGY 3 cut(s) 685, 3157, 6822
Cfr9I CCCGGG 1 cut(s) 7011
CseI GACGC 3 cut(s) 980, 3521, 5149
CspAI ACCGGT 1 cut(s) 6822
CspCI CAANNNNNGTGG 4 cut(s) 1673, 1708, 4233, 4268
DraI TTTAAA 2 cut(s) 160, 669
DraIII CACNNNGTG 2 cut(s) 5252, 5705
DrdI GACNNNNNNGTC 1 cut(s) 1789
DriI GACNNNNNGTC 2 cut(s) 6623, 7039
DseDI GACNNNNNNGTC 1 cut(s) 1789
EaeI YGGCCR 4 cut(s) 96, 313, 2595, 3979
Eam1104I CTCTTC 8 cut(s) 1285, 1536, 1699, 1797, 2913, 4605, 6836, 7115
Eam1105I GACNNNNNGTC 2 cut(s) 6623, 7039
EarI CTCTTC 8 cut(s) 1285, 1536, 1699, 1797, 2913, 4605, 6836, 7115
Ecl136II GAGCTC 1 cut(s) 6325
Eco130I CCWWGG 8 cut(s) 1198, 3038, 4903, 5147, 5764, 6531, 6830, 7455
Eco24I GRGCYC 3 cut(s) 2749, 4903, 6327
Eco31I GGTCTC 3 cut(s) 4261, 6053, 7026
Eco32I GATATC 3 cut(s) 2686, 6441, 7803
Eco53kI GAGCTC 1 cut(s) 6325
Eco57I CTGAAG 6 cut(s) 2307, 2805, 2981, 4863, 5475, 7253
Eco72I CACGTG 1 cut(s) 5696
Eco81I CCTNAGG 1 cut(s) 5155
Eco88I CYCGRG 1 cut(s) 7011
Eco91I GGTNACC 1 cut(s) 6818
EcoICRI GAGCTC 1 cut(s) 6325
EcoNI CCTNNNNNAGG 2 cut(s) 4547, 5674
EcoO109I RGGNCCY 9 cut(s) 2092, 2383, 2746, 2903, 4899, 4900, 5151, 6617, 7745
EcoO65I GGTNACC 1 cut(s) 6818
EcoRI GAATTC 2 cut(s) 584, 2952
EcoRII CCWGG 8 cut(s) 2182, 2637, 4469, 4955, 6172, 6631, 6681, 7395
EcoRV GATATC 3 cut(s) 2686, 6441, 7803
EcoT14I CCWWGG 8 cut(s) 1198, 3038, 4903, 5147, 5764, 6531, 6830, 7455
EcoT22I ATGCAT 3 cut(s) 385, 3227, 5810
EcoT38I GRGCYC 3 cut(s) 2749, 4903, 6327
ErhI CCWWGG 8 cut(s) 1198, 3038, 4903, 5147, 5764, 6531, 6830, 7455
FaqI GGGAC 9 cut(s) 4, 2916, 3376, 4577, 4675, 5059, 5271, 6512, 7216
FauI CCCGC 7 cut(s) 60, 1755, 3921, 5248, 5389, 5460, 7443
FauNDI CATATG 5 cut(s) 729, 1319, 2692, 3793, 7638
FbaI TGATCA 3 cut(s) 1059, 4267, 5745
FblI GTMKAC 8 cut(s) 1309, 2195, 2316, 2456, 5791, 6578, 6940, 7152
FriOI GRGCYC 3 cut(s) 2749, 4903, 6327
GlaI GCGC 3 cut(s) 2771, 6978, 6984
GsaI CCCAGC 5 cut(s) 2826, 3554, 7068, 7119, 7255
GsuI CTGGAG 6 cut(s) 311, 2205, 4086, 5025, 5441, 6665
HgaI GACGC 3 cut(s) 980, 3521, 5149
HhaI GCGC 3 cut(s) 2772, 6979, 6985
Hin1I GRCGYC 1 cut(s) 5160
Hin6I GCGC 3 cut(s) 2770, 6977, 6983
HinP1I GCGC 3 cut(s) 2770, 6977, 6983
HincII GTYRAC 5 cut(s) 1462, 2457, 2627, 2644, 6579
HindII GTYRAC 5 cut(s) 1462, 2457, 2627, 2644, 6579
HindIII AAGCTT 7 cut(s) 705, 1178, 3087, 4386, 5412, 5720, 6992
HpaI GTTAAC 1 cut(s) 2644
Hpy99I CGWCG 2 cut(s) 35, 4164
HpyCH4IV ACGT 8 cut(s) 2192, 2533, 3192, 4159, 5129, 5236, 5288, 5695
HpySE526I ACGT 8 cut(s) 2192, 2533, 3192, 4159, 5129, 5236, 5288, 5695
Hsp92I GRCGYC 1 cut(s) 5160
HspAI GCGC 3 cut(s) 2770, 6977, 6983
Kpn2I TCCGGA 1 cut(s) 6807
KroI GCCGGC 1 cut(s) 685
KroNI GCCGGC 1 cut(s) 687
Ksp22I TGATCA 3 cut(s) 1059, 4267, 5745
KspAI GTTAAC 1 cut(s) 2644
LguI GCTCTTC 1 cut(s) 7115
MaeII ACGT 8 cut(s) 2192, 2533, 3192, 4159, 5129, 5236, 5288, 5695
MfeI CAATTG 3 cut(s) 4010, 4680, 7733
MhlI GDGCHC 7 cut(s) 2749, 4444, 4903, 5701, 6327, 7133, 7720
MlsI TGGCCA 2 cut(s) 2597, 3981
MluNI TGGCCA 2 cut(s) 2597, 3981
MlyI GAGTC 9 cut(s) 26, 137, 1844, 2336, 5237, 5602, 5771, 5933, 7841
MmeI TCCRAC 7 cut(s) 35, 1963, 2784, 3873, 5903, 5991, 7009
Mox20I TGGCCA 2 cut(s) 2597, 3981
Mph1103I ATGCAT 3 cut(s) 385, 3227, 5810
MroI TCCGGA 1 cut(s) 6807
MroNI GCCGGC 1 cut(s) 685
MroXI GAANNNNTTC 7 cut(s) 383, 708, 744, 3052, 3921, 5089, 5988
MscI TGGCCA 2 cut(s) 2597, 3981
MslI CAYNNNNRTG 9 cut(s) 903, 2037, 2168, 3008, 3408, 7182, 7185, 7320, 7824
Msp20I TGGCCA 2 cut(s) 2597, 3981
MspA1I CMGCKG 6 cut(s) 1847, 2822, 3928, 4453, 7255, 7706
MspCI CTTAAG 4 cut(s) 1268, 2513, 6061, 7163
MunI CAATTG 3 cut(s) 4010, 4680, 7733
Mva1269I GAATGC 7 cut(s) 383, 385, 3225, 3580, 5939, 5965, 5973
MvaI CCWGG 8 cut(s) 2184, 2639, 4471, 4957, 6174, 6633, 6683, 7397
MvnI CGCG 1 cut(s) 5396
NaeI GCCGGC 1 cut(s) 687
NciI CCSGG 5 cut(s) 37, 2977, 6169, 7012, 7013
NdeI CATATG 5 cut(s) 729, 1319, 2692, 3793, 7638
NgoMIV GCCGGC 1 cut(s) 685
NmeAIII GCCGAG 4 cut(s) 874, 2350, 7291, 7829
NmuCI GTSAC 8 cut(s) 1116, 1561, 1656, 2779, 3509, 6751, 6818, 7293
NsiI ATGCAT 3 cut(s) 385, 3227, 5810
NspV TTCGAA 7 cut(s) 1702, 3046, 5533, 5887, 7198, 7502, 7607
PaeI GCATGC 6 cut(s) 902, 2296, 5911, 6008, 7115, 7727
PagI TCATGA 3 cut(s) 243, 2983, 7180
PaqCI CACCTGC 1 cut(s) 6826
PciI ACATGT 2 cut(s) 304, 5260
PciSI GCTCTTC 1 cut(s) 7115
PcsI WCGNNNNNNNCGW 2 cut(s) 79, 1309
PctI GAATGC 7 cut(s) 383, 385, 3225, 3580, 5939, 5965, 5973
PdiI GCCGGC 1 cut(s) 687
PdmI GAANNNNTTC 7 cut(s) 383, 708, 744, 3052, 3921, 5089, 5988
PflFI GACNNNGTC 1 cut(s) 16
PflMI CCANNNNNTGG 1 cut(s) 6794
PfoI TCCNGGA 1 cut(s) 7395
PinAI ACCGGT 1 cut(s) 6822
PleI GAGTC 9 cut(s) 25, 136, 1844, 2335, 5236, 5602, 5771, 5932, 7841
PmaCI CACGTG 1 cut(s) 5696
PmlI CACGTG 1 cut(s) 5696
PpsI GAGTC 9 cut(s) 25, 136, 1844, 2335, 5236, 5602, 5771, 5932, 7841
Ppu21I YACGTR 3 cut(s) 2534, 5237, 5696
PpuMI RGGWCCY 5 cut(s) 2383, 2903, 5151, 6617, 7745
PscI ACATGT 2 cut(s) 304, 5260
PshAI GACNNNNGTC 1 cut(s) 730
PshBI ATTAAT 2 cut(s) 3687, 3956
PsiI TTATAA 3 cut(s) 1716, 4916, 7482
Psp124BI GAGCTC 1 cut(s) 6327
Psp5II RGGWCCY 5 cut(s) 2383, 2903, 5151, 6617, 7745
Psp6I CCWGG 8 cut(s) 2182, 2637, 4469, 4955, 6172, 6631, 6681, 7395
PspCI CACGTG 1 cut(s) 5696
PspEI GGTNACC 1 cut(s) 6818
PspFI CCCAGC 5 cut(s) 2822, 3550, 7064, 7115, 7251
PspGI CCWGG 8 cut(s) 2182, 2637, 4469, 4955, 6172, 6631, 6681, 7395
PspOMI GGGCCC 2 cut(s) 2745, 4899
PspPPI RGGWCCY 5 cut(s) 2383, 2903, 5151, 6617, 7745
PsrI GAACNNNNNNTAC 2 cut(s) 6016, 6048
PstI CTGCAG 4 cut(s) 1930, 3141, 3328, 3430
PstNI CAGNNNCTG 7 cut(s) 1337, 3323, 3920, 4453, 4465, 4892, 7276
PsyI GACNNNGTC 1 cut(s) 16
PvuII CAGCTG 3 cut(s) 2822, 4453, 7255
RseI CAYNNNNRTG 9 cut(s) 903, 2037, 2168, 3008, 3408, 7182, 7185, 7320, 7824
SacI GAGCTC 1 cut(s) 6327
SalI GTCGAC 2 cut(s) 2455, 6577
SapI GCTCTTC 1 cut(s) 7115
ScaI AGTACT 2 cut(s) 2122, 5452
SchI GAGTC 9 cut(s) 26, 137, 1844, 2336, 5237, 5602, 5771, 5933, 7841
SduI GDGCHC 7 cut(s) 2749, 4444, 4903, 5701, 6327, 7133, 7720
SfuI TTCGAA 7 cut(s) 1702, 3046, 5533, 5887, 7198, 7502, 7607
SmaI CCCGGG 1 cut(s) 7013
SmiMI CAYNNNNRTG 9 cut(s) 903, 2037, 2168, 3008, 3408, 7182, 7185, 7320, 7824
SmlI CTYRAG 9 cut(s) 589, 598, 656, 1268, 2513, 5013, 6061, 6967, 7163
SmoI CTYRAG 9 cut(s) 589, 598, 656, 1268, 2513, 5013, 6061, 6967, 7163
SpeI ACTAGT 2 cut(s) 2123, 6755
SphI GCATGC 6 cut(s) 902, 2296, 5911, 6008, 7115, 7727
SspI AATATT 4 cut(s) 156, 2062, 4640, 6675
SstI GAGCTC 1 cut(s) 6327
StyI CCWWGG 8 cut(s) 1198, 3038, 4903, 5147, 5764, 6531, 6830, 7455
TaiI ACGT 8 cut(s) 2195, 2536, 3195, 4162, 5132, 5239, 5291, 5698
TaqII GACCGA 1 cut(s) 7274
TauI GCSGC 5 cut(s) 315, 5683, 5840, 6374, 6725
TseFI GTSAC 8 cut(s) 1116, 1561, 1656, 2779, 3509, 6751, 6818, 7293
Tsp45I GTSAC 8 cut(s) 1116, 1561, 1656, 2779, 3509, 6751, 6818, 7293
TspGWI ACGGA 9 cut(s) 19, 2213, 3497, 3732, 4012, 4809, 5339, 7377, 7813
TspMI CCCGGG 1 cut(s) 7011
Tth111I GACNNNGTC 1 cut(s) 16
Van91I CCANNNNNTGG 1 cut(s) 6794
Vha464I CTTAAG 4 cut(s) 1268, 2513, 6061, 7163
VneI GTGCAC 1 cut(s) 5697
VspI ATTAAT 2 cut(s) 3687, 3956
XagI CCTNNNNNAGG 2 cut(s) 4547, 5674
XbaI TCTAGA 2 cut(s) 389, 2714
XmaI CCCGGG 1 cut(s) 7011
XmaJI CCTAGG 2 cut(s) 1198, 3038
XmiI GTMKAC 8 cut(s) 1309, 2195, 2316, 2456, 5791, 6578, 6940, 7152
XmnI GAANNNNTTC 7 cut(s) 383, 708, 744, 3052, 3921, 5089, 5988
ZrmI AGTACT 2 cut(s) 2122, 5452
Zsp2I ATGCAT 3 cut(s) 385, 3227, 5810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.