Rw3G001200

Translational activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0004626
Physical Location & Seq
Forward (+)
968329 .. 993447
25119 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G001200.1

Sequence Viewer

Length: 8127 bp
ATGTCGTACAACATGTCATCTTCTTCCAAAGAAGCCTCCCAACTTACAAAGCCATTTCCACCCATCATAATTCCTATATCATTCAGCTCTAAGAATTCCCTCTCCCCACAAACTCCAGACCAATCAGCACTCAACACAGGATTACTCCCCCACAAGCAATCATTCTCTCTATTATTACTACTCCAACCTCCATCTGAAAAAACAAACCTCCATCTTTTTCACAATCGGATCCTCCATTCGGATCCGCCCCGCCCGTTTCTCCCAATGGCGGCTCCGCCAGAGTCTCTTCTCTCCGTCGCCGGGTCGGTCTCCACCGCCTCCACTCAGAAGCGGGTTCGGATCTTCCGAGATGAAATTCCGGCCATTATCGACGGCTCCGAAATATGTGTGGAGTCTGCAACACTGTTGGTAGATATAATATTTAAAACATTGTACATTTATGAGGATCGTGGGTCCAAGAAAGCTGTCGATGACATTATCACGAAATGTTTTCAAGAAGTTGCCTTCATGAAGAATTTTGCAGCAGCCCTTGTTCAGATGATGGAAAAAAATGTAAGGGTGCAATCACATGTTGGAGGCCACAGACTTTTCCAGTGGTCTTGTCTTCTTCTTAGCAAGAGTAAATTCGCCACAGTTTCCAAGAATGCATTCTGTAGAGTTGGCACAGTGCAGGCATCATTACTTCATATTGTTGTACAAACATCTCTCAGTGAGCAACGGGCTTGCAAGAAGACATTTTATCACTTACTTTCTCAGTCGCCGGACATCTACAAGTTGTATATAGAGGAGTTGAAGGAAGCACGCATGCCATACAAAGATAGTCCTGAATTATTATGGTTCTTGATGGAATTCTCAAGTACCTCAAGAAAATGCTTCTTTCCAATATTTCTTGATATATATCTCAAGGCAATTTTAAACGCAAAAGAAAAGCCAGCAAAGGGTCTTAGCGAAGCTTTTTGTCCACTTTTTCGGCATATGTTGCATGAAGATTTTCAAAATGTTGTGCTTCCATCTTCTGTTAAAATGCTGAAACGCAACCCTGAGATTGTATTGGAATCAGTTGGCATTCTCTTGAAGTCAGTCAACCTTGATTTGAGCAAGTATGCTGTCGAAATCCTTTCACTGGTCTTGCCACAGGCTCGGCATGCAGATGAAGGCCGGAGGGTTGGTGCACTGGAAATTGTACGATGTCTGAGCCAAAATTCTAGTAATCCAGATGCTATAGAAGCAATGGTCAATGCTGTCAAATCTGTCCTTGGAGGCTCAGAGGGAAGACTTACATTTCCTTACCAGAGAATTGGCATGATCAATGCACTGCAAGAATTGTGTAATGCTCCAGATGGGAAGCACCTTAATAGACTGTCACAGACCTCATGCAATTACCTTCGATCTTGTTACAAGGAGGATGGCAATGAGGAGGTCAAGCTTGCAATTTTGTCAGCTCTAGGTTTGTGGGCAGCAAGATCTGCTGATGTTGTTCAGTCAGATTTAGTTTCTTTTTTGTCTTCTGGGCTTAAGGAGAAAGAAGCTCTAAGAAGAGGGCATCTTCGTTGCCTAAGAGCCATATGCAGAATACAGATGCTGTTTATCGGGTCAGCTCGTGGTTTTAGTTTTATTTGGCAGTTCTTTTTATCATCATTACTAGAACCTCTCATTCAACTTGTCAAAACTGGTTTTACTAAAGGAGTTCAGCGTTTGGATGGTATATATGCCTTGCTATTAGTTGGCAAAATTGCAGCAGTTGACATCAAAGCAGAGGAGACCGTGATGAAGGAAAAGATCTGGTCTGTGATATCTCAAAATGAGCCATCCCTTCTTCCAATATCCTTGGCTTCAAAATTGTTGACTGAGGACTGCATGGCATGTGTCGATCTTCTTGAAGTCATGCTGGTGGAACATTTGCAGAGGGCGTTGGATTCCTTTTCTGTCAGATCACTGTCACAGTTGATAATATTTTTCATGTGCCACCCATGTTGGGATATTCGAAGAATGGCTTATAATGCTACCAAAAAGATAGTTGCTGCTGCCCCACAATTAGCTGAACATCTTTTGATTGAATTCACGACTTTCATGTCTGTAGTTGAGGAGAAACATCGTATTTCAAAGTCAAGTGATACAGATAGTTCATTGGACTCCCAAGTACCGTTCCTTCCCTCGGTAGAGGTTTCAGTAAAGGCTTTACTTGTTATATCATCAGCAGTTCTACCTGCAGCTCCAAGTGCATCAATGCGAGTTTTATTATGCGGACATCATCCATACTTAGGTTGGAACTGCAAAAAGAGATGCAGTTTGGAGGGTAAGTTTTTCTGTAGGCTACACAAATGTTTGCATAAATGTGGTTTCGACATAAACAGCAATATTTTGGCTGACATGGAGAAATTGTGCAAGGGCCTACTGGAAACAATGTGGTTGTCCAGTACTACTCAATCAGAGCAGCAAGCAGCAATATCCTCATTAGCCACTCTGATGTCCATTGCACCTGGAGAAACGTATGTGGAGTTTGAAAAGCACTTGAAACACCTTCCTTACCGATATTCACATGATATGCTTTCCGAAAATGATATTCGGATTTTTCATACTCCTGAAGGCTTGCTTTCCAGTGAGCAAGGCGTCTACATAGCTGAGTCTGTTGCTGCCAAGAACATGAAACAAGCTAAGGGCCGTTTTCGGATGTATGAGGACCTAAATGACATGGATAATGGTGGTTCTAATCATTCTGCAAAAGTCGATCCAAGCAGTAAAACTGGGAAGTCGACAAAGAAACCTGAGAAAACAAAGACTGCAAAAGAGGAGGCACGTGAGTTGCAACTTAAGGAGGAGGCATCCACACGTGAGAAGGTCCGAGAAATACAGAAAAACCTGTCTTTAATGCTTAAAGCTCTTGGGGAAATGGCCATTGCAAATCCTGTTTTTGCACATAGTCGACTTCCTTCCCTGGTTAACTATGTCAATCCGTTACTAAGGTCACCAATAGTCAGCGATATTGCATATGAAACTATGGTGAAACTCTCTAGATGTACTGCTCCCCCTCTTTGTAATTGGGGCCTTGACATTGCTACTGCTTTGCGCTTAGTTGTGACTGAAGAAGATCGTCTTCTGTTGGATATGATTTCATCGGTTGGGCAAGGGGAAGCCGATGATAGACCATCTTTGAGTCTATTTGAGCGAATTATTAACGCCATTTCTGCATCTTGTAAATCGGGACCTCTTCCTGTTGATTCCTTCACTTTTGTCTTCCCTATAATGGAGCGAATTCTTTTAAGCTCTAAGAAGACCGGGCTTCATGATAATGTGCTTCAAATTATTTACTTGCACATGGACCCCCTATTACCCCTCCCTAGGCTTCGAATGATTTCAGTTCTTTATCATGTTCTTGGTATTGTACAAGCTTATCAATCCTCTATTGGACCTGCGTTGAATGAGTTGTGTCTTGGTCTGCAGCCAGATGAAGTTGCACCGGCTTTATACGGGGTTTATGCAAAAGATGTTCATGTTAGAATGGCTTGTCTAACTGCTGTCAAATGCATTCCTACTGTTGCTAGTCGGTCTCTTATTCAGAATGTTGAGGTTGCTACAAGTATTTGGATTGCTTTACATGACCCAGAAAAGTCAGTTGCAGAAGCTGCAGAGGATTTGTGGGATCGCTATGGACATGACTTTGGGACTGACTATTCAGGCCTTTTCAAAGCACTTTCTCATATCCATTATACTGTCCGTTTTGCTGCTGCAGAGGCATTAGCTGCTGCTTTGGACGAAAATCCTGATTCTATACAGGAATCCCTTTCTACTTTATTCTCCCTGTATATCCGTGATGCTGGTTTGACTGAGAACAATGTCGATGCTGGTTGGCTGGGCCGGCAGGGTGTAGCTTTGGCATTGCATTCTTCTGCTGATGTACTGAGAACAAAAGACCTTCCTGTTGTTATGACCTTTTTGATTTCAAGAGCATTGGCAGATCCTAATGCTGATGTTCGGGGGAGAATGATTAATGCTGGCATTATGATTATTGATAAGCACGGAAGAGATAACGTGTCCTTGCTATTTCCCATCTTTGAGAACTACTTAAACAAAAAGGCATCGGATGAGGAGACATATGACTTGGTTCGTGAGGGTGTTGTTATTTTCACTGGAGCGTTGGCAAAGCATTTGGCAAAGGATGATCCAAAAGTACATGCTGTTATAGAGAAGTTGTTGGATGTGTTGAACACTCCATCAGAAGCTGTTCAGCGGGCAGTTTCAACATGCCTATCACCATTAATGCAATCAAAGCAAGATGATGCCCAAGCTCTTGTTTCAAGGATTTTGGATCAATTGATGAACAGTGATAAGTATGGGGAACGCCGTGGAGCTGCTTTTGGGCTTGCTGGAGTAGTCAAGGGATTTGGTATTTCTTCCTTGAAGAAGTATGGAATTGTGAATATACTACAGGAAGGACTTGTTGATAGGAATTCTGCAAAACGTCGTGAAGGGGGTTTGCTTGGATTTGAGTGTCTATGTGAAACACTTGGAAAATTATTTGAACCGTATGTAATACAAATGTTGCCACTACTGTTGGTTTCATTTTCTGATCAAGTTGTTGCAGTTCGTGAAGGTGCTGAGTGTGCTGCTCGGGCAATGATGTCTCACCTTACTGCTCAGGGAGTGAAACTGGTCCTTCCATCTCTTTTGAAGGGTCTTGAAGATAAAGCTTGGCGAACAAAGCAAAGTAGTGTTCAACTGCTTGGTGCTATGGCCTATTGTGCTCCTAAGCAGCTGTCTCAATGTCTCCCTAAGATTGTACCGAAACTGACTGAGGTTTTAACAGACACCCACCCTAAAGTTCAGTCAGCAGGACAAACAGCACTTCAACAGGTTGGGAGCGTAATAAAGAATCCTGAGATAGCTTCATTGGTCCCTACTCTTCTTCTGGGTCTTACAGATCCAAATGACTATACAAAATATTCTCTGGATGTTCTCTTGGGAACAACATTCATCAATTCAATTGATGCTCCTTCACTTGCACTCTTGGTGCCGATAGTACATAGAGGGCTAAGGGAGAGGGGTGCAGAGACTAAAAAGAAAGCAGCCCAAATAGTTGGCAATATGTGTTCGTTAGTTACAGATCCAAATGATATGATTCCGTACATAGGGCTGCTTCTTCCTGAAGTGAAAAAGGTTCTTGTGGATCCAATTCCAGAAGTTAGGTCTGTTGCTGCAAGGGCGCTTGGATCCCTCATAAGGGGAATGGGCGAGGATCATTTCCCAGATCTTGTTCCCTGGTTGTTGGATACACTTAAATCAGATAATAGTAATGTTGAGCGATCTGGAGCTGCTCAAGGGTTAAGTGAGGTATTAGCTGCACTTGGGACTGAGTACTTTGAGCATGTACTTCCTGATGTTATCCGAAATTGTTCTCATCAAAAAGCATCTGTGCGTGATGGGCATTTGACATTATTCAAGTATTTACCAAGGTCCTTAGGCGTCCAATTCCAGAATTATTTACAGCAGGTGCTGCCTGCTATTATAGATGGTCTTGCTGATGAAAATGAGTCTGTACGTGAAGCAGCATTAGGTGCGGGACATGTTCTGGTTGAGCATTATGCTATAACGTCGTTACCTTTGCTCCTACCCGCTGTTGAGGACGGTATCTTTAATGATAGTTGGCGAATTCGACAAAGCTCTGTGGAATTATTGGGGGATCTCTTATTTAAGGTCGCGGGTACTTCTGGAAAAGCTTTACTGGAGGGTGGCAGTGATGATGAAGGTGCAAGTACTGAAGCACAGGGGCGGGCTATCATTGAGGTTCTTGGAAGGGATAAGCGTGATGAAATTCTCGCTGCATTATATATGGTTCGAACTGATGTCAGCCTATCAGTGCGTCAGGCTGCGTTGCATGTATGGAAAACTATAGTGGCAAATACCCCAAAAACTCTAAAGGAAATAATGCCAGTTCTAATGAATACTCTAATTACCTCTCTTGCATCATCATCCTTTGAAAGGCGGCAGGTCGCTGGACGTGCACTGGGTGAGCTTGTCAGGAAGCTTGGTGAAAGAGTGCTGCCATTGATCATCCCAATTTTATCCCAAGGATTGAAGGACTCTGACACCAGTAGACGACAAGGTGTATGCATTGGTCTGAGTGAAGTAATGGGATCTGCAGCTAAAAGTCATTTGTTGAGTTTCATGGATGAGCTCATTCCAACCATTCGAACTGCTCTTTCAGATAGCATGCCCGAAGTTCGTGAGTCTGCTGGAATAGCATTCAGCACTCTGTACAAGAATGCTGGAATGCAAGCGATTGATGAAATTGTTCCCAGTCTTTTGCATGCTTTGGAGGACGATCAAACTTCTGATACTGCACTTGATGTTTTACCCCACATTTTGCCCAAGCTTGTCCAGCTTCCACTAACGTATGTTTTCTTTTCTCGAACTTACTCTGTAGCTGAGGTTGCTGGGCCTGGTCTTAACACTCATCTTGGTACCGTTCTTCCTGCTTTACTTACTTCAATGGGTGTTGATGCAAAGGATGTTCAAACATTGGCAAAGGAGGCTGCTGAAACTGTGGTCTTAGTTATAGATGAGGAAGGTGTTGAATATCTTACATCTGAGCTACTTAGAGCTGTCAGCGATAGTCAGGCTTCAATTAGAAGAAGTGCCGCGTATCTTATAGGCTATTTCTTCAAAAACAGTAAATTATATTTGGTTGATGAAGCACCAAACATGATATCTACCTTGATTGTCTTGCTCAGTGATTCTGATTCGGCTACTGTGGCGGTATCTTGGGAAGCTTTGTCAAGGGTTGTAAGTTCAGTCCCCAAGGAAGTACTTCCTTCATATATTAAATTAGTACGCGATGCTGTGTCGACTTCCAGAGATAAGGAGCGCAGGAAAAAGAAGGGAGGACCTATTGTCATACCTGGATTTTGTTTACCAAAAGCACTTCAACCTTTGCTTCCAATATTTCTCCAGGGTTTAATAAGTGGATCTGCTGAACTGAGAGAGCAAGCGGCACTGGGTCTTGGGGAACTGATCGAAGTGACTAGTGAAAAGGCACTCAAAGAGTTTGTCATTCCAATTACAGGGCCTCTTATCCGGATAATAGGTGACCGGTTCCCTTGGCAGGTGAAGAGTGCTATTTTATCTACCTTGACAATCATAATCAGAAAAGGTGGGATGGCCTTGAAACCATTTCTTCCGCAGCTTCAAACAACATTTGTCAAGTGCCTACAGGATAACACAAGAATTGTTCGCTCAAGTGCCGCCCTTGCTCTCGGAAAGCTTAGTGCTTTAAGTACCCGGGTGGACCCTCTAGTTGGAGACCTCCTGTCCAGTCTGCAGGCTATGGATAGTGGGGTTCGGGAGGCAAGTTTGTCTGCTTTAGAAGGTGTCCTAAAGTATGCTGGAAAGAGCGTGAGCAGTGCTGTTAGAACTCGTGTCTACTTGCAGCTTAAGGATTTGATTCATCATGATGATGATGAGGTTCGAGTATCTGCTGCAAGCATTTTGGGTATCATGTCCCAGTACATTGAAGATACCCAGCTGATCGAGCTGCTTCAGGAACTATCAAGTTTTCCATCGTCGCTCAGCTGGACTGCTAGGCATGGTTCTGTACTTACAATTTCATCCATGCTCAGGCACATCCCATCCACCATCTGTACTTCTCCAGTGTTTCCGTCTATCCTGGACCAGCTTAAAGCTGCCTTGACTGATGAGAAGTTCCCTCTTCGTGAAACTTCAACCAAGGCATTTGGGAGACTTCTTGTTTATAAAGTCCGAAGTGATCCTTCTAATACCTCAGTACAATTAGATATTATATCGTCTTTAGTCTCAGCATTGCATGATGACTCTAGCGAAGTTAGAAGAAAGGCGTTATCTGCTCTAAAGGCAGTTTCGAAGGAAAGTTCTTCACCTATTACGGCCCATATCAATATTATTGGACCTGCACTGGCTGAATGTTTGAAGGATGGAAGTACACCCGTGAGACTTGCAGCAGAAAGATGTGCTCTGCATGCCTTCCAATTGACAAAGGGTCCTGAAAATGTTCAAGCTGCACAAAAGTTCATCACAGGCTTGGAAGCTAGACGAATATCAAAGTTTTCAGAACATAGTGATGATAGTGGAGGTGAGGATGACTTGGCAAGTGGCTGA

Protein Analysis

2708

Amino Acids

297.03

Weight (kDa)

6.81

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GNC1_N PF24993 295 - 426 3e-44 Stalled ribosome sensor GCN1 N-terminal
HEAT_EF3_N PF24987 1447 - 1614 1.4e-46 EF3 N-terminal HEAT repeat
HEAT_GCN1 PF23271 1497 - 1634 2.8e-10 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_GNC1 PF24984 1652 - 1738 6e-26 Elongation factor 3/GNC1 HEAT repeat
HEAT_GCN1 PF23271 1726 - 1873 2.1e-11 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_N PF24987 1767 - 1896 2.8e-08 EF3 N-terminal HEAT repeat
TPR_DNAAF5 PF25757 1935 - 2090 7e-06 Dynein axonemal assembly factor 5-like, TPR repeats
HEAT_GCN1 PF23271 1969 - 2105 4.3e-06 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_N PF24987 1982 - 2077 3.7e-06 EF3 N-terminal HEAT repeat
HEAT_GCN1 PF23271 2116 - 2305 4.6e-45 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_MROH2B_C PF23227 2399 - 2508 1.3e-06 MROH2B-like, HEAT repeats
HEAT_GCN1_C_2 PF25801 2428 - 2679 3e-31 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_GCN1_C PF25786 2526 - 2680 2.2e-74 GCN1-like, C-terminal HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1998, 7746
AarI CACCTGC 2 cut(s) 5476, 7090
AasI GACNNNNNNGTC 1 cut(s) 2071
Acc36I ACCTGC 6 cut(s) 2215, 3418, 5476, 5974, 7090, 7927
Acc65I GGTACC 1 cut(s) 6458
AccB1I GGYRCC 2 cut(s) 5008, 6458
AccI GTMKAC 6 cut(s) 2613, 2753, 2923, 6091, 6842, 7416
AccII CGCG 3 cut(s) 5696, 6638, 6831
AccIII TCCGGA 1 cut(s) 7071
AcoI YGGCCR 2 cut(s) 360, 2892
AcuI CTGAAG 5 cut(s) 2604, 3102, 5163, 5775, 7517
AcvI CACGTG 2 cut(s) 2798, 2831
AcyI GRCGYC 2 cut(s) 2610, 5460
AdeI CACNNNGTG 1 cut(s) 6005
AflII CTTAAG 3 cut(s) 1514, 2810, 7427
AflIII ACRYGT 5 cut(s) 12, 568, 2828, 4029, 5560
AgeI ACCGGT 1 cut(s) 7086
AhdI GACNNNNNGTC 3 cut(s) 6887, 7303, 8007
AhlI ACTAGT 1 cut(s) 7019
AjiI CACGTC 1 cut(s) 5996
AjnI CCWGG 7 cut(s) 2479, 2934, 5255, 6436, 6895, 6945, 7659
AjuI GAANNNNNNNTTGG 2 cut(s) 632, 664
Alw21I GWGCWC 5 cut(s) 1174, 4744, 6001, 6174, 7984
Alw44I GTGCAC 2 cut(s) 1170, 5997
AlwNI CAGNNNCTG 4 cut(s) 1582, 3623, 4220, 5491
Ama87I CYCGRG 2 cut(s) 4608, 7275
Aor13HI TCCGGA 1 cut(s) 7071
ApaLI GTGCAC 2 cut(s) 1170, 5997
ArsI GACNNNNNNTTYG 2 cut(s) 7029, 7061
AseI ATTAAT 2 cut(s) 3987, 4256
AsiGI ACCGGT 1 cut(s) 7086
Asp700I GAANNNNTTC 8 cut(s) 647, 990, 3352, 4221, 5389, 6288, 6804, 8019
Asp718I GGTACC 1 cut(s) 6458
AspA2I CCTAGG 1 cut(s) 3338
AspLEI GCGC 3 cut(s) 3069, 5203, 6864
AsuC2I CCSGG 4 cut(s) 301, 3277, 7276, 7277
AsuII TTCGAA 5 cut(s) 1984, 3346, 5833, 6187, 7871
AvaI CYCGRG 2 cut(s) 4608, 7275
AvrII CCTAGG 1 cut(s) 3338
AxyI CCTNAGG 1 cut(s) 5455
BaeGI GKGCMC 2 cut(s) 1174, 6001
BalI TGGCCA 1 cut(s) 2894
BamHI GGATCC 4 cut(s) 228, 241, 5164, 5207
BanI GGYRCC 2 cut(s) 5008, 6458
BanII GRGCYC 1 cut(s) 6174
BarI GAAGNNNNNNTAC 2 cut(s) 2133, 2165
BauI CACGAG 2 cut(s) 1599, 7410
BbrPI CACGTG 2 cut(s) 2798, 2831
BbsI GAAGAC 7 cut(s) 596, 737, 1279, 1497, 3086, 3226, 3278
Bbv12I GWGCWC 5 cut(s) 1174, 4744, 6001, 6174, 7984
BbvCI CCTCAGC 1 cut(s) 6423
BceAI ACGGC 4 cut(s) 388, 2646, 4326, 7911
BciT130I CCWGG 7 cut(s) 2481, 2936, 5257, 6438, 6897, 6947, 7661
BciVI GTATCC 1 cut(s) 5260
BclI TGATCA 3 cut(s) 1305, 4567, 6045
BcnI CCSGG 4 cut(s) 301, 3277, 7276, 7277
BcuI ACTAGT 1 cut(s) 7019
BfoI RGCGCY 1 cut(s) 5204
BfrI CTTAAG 3 cut(s) 1514, 2810, 7427
BfuAI ACCTGC 6 cut(s) 2215, 3418, 5476, 5974, 7090, 7927
BfuI GTATCC 1 cut(s) 5260
BglII AGATCT 3 cut(s) 1463, 1779, 5245
BlnI CCTAGG 1 cut(s) 3338
BlpI GCTNAGC 1 cut(s) 7562
BmcAI AGTACT 4 cut(s) 2419, 5354, 5752, 6804
BmeRI GACNNNNNGTC 3 cut(s) 6887, 7303, 8007
BmeT110I CYCGRG 2 cut(s) 4608, 7275
BmgBI CACGTC 1 cut(s) 5996
BmrI ACTGGG 5 cut(s) 2754, 6011, 6288, 7001, 7492
BmuI ACTGGG 5 cut(s) 2754, 6011, 6288, 7001, 7492
BpiI GAAGAC 7 cut(s) 596, 737, 1279, 1497, 3086, 3226, 3278
BpmI CTGGAG 9 cut(s) 99, 1320, 2502, 4149, 4386, 5325, 5741, 6929, 7626
Bpu10I CCTNAGC 6 cut(s) 2655, 4635, 4746, 5030, 6423, 7610
Bpu1102I GCTNAGC 1 cut(s) 7562
Bpu14I TTCGAA 5 cut(s) 1984, 3346, 5833, 6187, 7871
BpuEI CTTGAG 5 cut(s) 838, 847, 887, 5298, 7216
BpuMI CCSGG 4 cut(s) 301, 3277, 7276, 7277
BsaAI YACGTR 3 cut(s) 2798, 2831, 5537
BsaBI GATNNNNATC 1 cut(s) 897
BsaHI GRCGYC 2 cut(s) 2610, 5460
BsaI GGTCTC 4 cut(s) 313, 1754, 3552, 7290
BsaWI WCCGGW 2 cut(s) 7071, 7086
BsaXI ACNNNNNCTCC 8 cut(s) 1153, 1183, 2285, 2315, 2366, 2396, 5586, 5616
Bse118I RCCGGY 3 cut(s) 3457, 3855, 7086
Bse21I CCTNAGG 1 cut(s) 5455
Bse3DI GCAATG 8 cut(s) 1236, 1417, 2472, 2895, 3051, 3875, 4620, 7811
Bse8I GATNNNNATC 1 cut(s) 897
BseAI TCCGGA 1 cut(s) 7071
BseBI CCWGG 7 cut(s) 2481, 2936, 5257, 6438, 6897, 6947, 7661
BseJI GATNNNNATC 1 cut(s) 897
BseMI GCAATG 8 cut(s) 1236, 1417, 2472, 2895, 3051, 3875, 4620, 7811
BseRI GAGGAG 7 cut(s) 800, 1430, 1772, 2099, 2804, 2831, 4100
BseSI GKGCMC 2 cut(s) 1174, 6001
BseYI CCCAGC 3 cut(s) 3850, 6431, 7515
BsgI GTGCAG 6 cut(s) 689, 5064, 5322, 6321, 7905, 8013
Bsh1236I CGCG 3 cut(s) 5696, 6638, 6831
BshNI GGYRCC 2 cut(s) 5008, 6458
BshTI ACCGGT 1 cut(s) 7086
BsiHKAI GWGCWC 5 cut(s) 1174, 4744, 6001, 6174, 7984
BsiHKCI CYCGRG 2 cut(s) 4608, 7275
BslFI GGGAC 7 cut(s) 3216, 3676, 4877, 5359, 5571, 6776, 7480
BsmFI GGGAC 7 cut(s) 3216, 3676, 4877, 5359, 5571, 6776, 7480
BsmI GAATGC 8 cut(s) 647, 649, 1065, 3525, 3880, 6239, 6265, 6273
Bso31I GGTCTC 4 cut(s) 313, 1754, 3552, 7290
BsoBI CYCGRG 2 cut(s) 4608, 7275
Bsp119I TTCGAA 5 cut(s) 1984, 3346, 5833, 6187, 7871
Bsp1286I GDGCHC 5 cut(s) 1174, 4744, 6001, 6174, 7984
Bsp13I TCCGGA 1 cut(s) 7071
Bsp1407I TGTACA 4 cut(s) 432, 694, 3382, 6252
Bsp1720I GCTNAGC 1 cut(s) 7562
BspEI TCCGGA 1 cut(s) 7071
BspFNI CGCG 3 cut(s) 5696, 6638, 6831
BspHI TCATGA 3 cut(s) 507, 3283, 7444
BspMAI CTGCAG 6 cut(s) 2212, 3441, 3628, 3730, 6139, 7317
BspMI ACCTGC 6 cut(s) 2215, 3418, 5476, 5974, 7090, 7927
BspT104I TTCGAA 5 cut(s) 1984, 3346, 5833, 6187, 7871
BspT107I GGYRCC 2 cut(s) 5008, 6458
BspTI CTTAAG 3 cut(s) 1514, 2810, 7427
BspTNI GGTCTC 4 cut(s) 313, 1754, 3552, 7290
BsrDI GCAATG 8 cut(s) 1236, 1417, 2472, 2895, 3051, 3875, 4620, 7811
BsrFI RCCGGY 3 cut(s) 3457, 3855, 7086
BsrGI TGTACA 4 cut(s) 432, 694, 3382, 6252
BssAI RCCGGY 3 cut(s) 3457, 3855, 7086
BssNI GRCGYC 2 cut(s) 2610, 5460
BssSI CACGAG 2 cut(s) 1599, 7410
BssT1I CCWWGG 8 cut(s) 1255, 1827, 3338, 5447, 6064, 6795, 7094, 7719
Bst2BI CACGAG 2 cut(s) 1599, 7410
Bst2UI CCWGG 7 cut(s) 2481, 2936, 5257, 6438, 6897, 6947, 7661
Bst6I CTCTTC 7 cut(s) 291, 1531, 3213, 4015, 4905, 7100, 7707
BstACI GRCGYC 2 cut(s) 2610, 5460
BstAFI CTTAAG 3 cut(s) 1514, 2810, 7427
BstAPI GCANNNNNTGC 6 cut(s) 979, 1466, 3623, 3740, 5491, 5552
BstAUI TGTACA 4 cut(s) 432, 694, 3382, 6252
BstBAI YACGTR 3 cut(s) 2798, 2831, 5537
BstBI TTCGAA 5 cut(s) 1984, 3346, 5833, 6187, 7871
BstDSI CCRYGG 1 cut(s) 4342
BstEII GGTNACC 2 cut(s) 2964, 7082
BstENI CCTNNNNNAGG 1 cut(s) 5974
BstFNI CGCG 3 cut(s) 5696, 6638, 6831
BstH2I RGCGCY 1 cut(s) 5204
BstHHI GCGC 3 cut(s) 3069, 5203, 6864
BstNI CCWGG 7 cut(s) 2481, 2936, 5257, 6438, 6897, 6947, 7661
BstPI GGTNACC 2 cut(s) 2964, 7082
BstSLI GKGCMC 2 cut(s) 1174, 6001
BstUI CGCG 3 cut(s) 5696, 6638, 6831
BstV2I GAAGAC 7 cut(s) 596, 737, 1279, 1497, 3086, 3226, 3278
BstXI CCANNNNNNTGG 2 cut(s) 1298, 5075
Bsu36I CCTNAGG 1 cut(s) 5455
BsuI GTATCC 1 cut(s) 5260
BtgI CCRYGG 1 cut(s) 4342
BtgZI GCGATG 1 cut(s) 6846
BtrI CACGTC 1 cut(s) 5996
BtsI GCAGTG 3 cut(s) 1313, 5737, 7402
BveI ACCTGC 6 cut(s) 2215, 3418, 5476, 5974, 7090, 7927
CaiI CAGNNNCTG 4 cut(s) 1582, 3623, 4220, 5491
CciI TCATGA 3 cut(s) 507, 3283, 7444
CfoI GCGC 3 cut(s) 3069, 5203, 6864
Cfr10I RCCGGY 3 cut(s) 3457, 3855, 7086
Cfr9I CCCGGG 1 cut(s) 7275
CseI GACGC 3 cut(s) 2599, 5449, 5846
CspAI ACCGGT 1 cut(s) 7086
CspCI CAANNNNNGTGG 4 cut(s) 1955, 1990, 4533, 4568
DraI TTTAAA 2 cut(s) 424, 915
DraIII CACNNNGTG 1 cut(s) 6005
DrdI GACNNNNNNGTC 1 cut(s) 2071
DriI GACNNNNNGTC 3 cut(s) 6887, 7303, 8007
DseDI GACNNNNNNGTC 1 cut(s) 2071
EaeI YGGCCR 2 cut(s) 360, 2892
Eam1104I CTCTTC 7 cut(s) 291, 1531, 3213, 4015, 4905, 7100, 7707
Eam1105I GACNNNNNGTC 3 cut(s) 6887, 7303, 8007
EarI CTCTTC 7 cut(s) 291, 1531, 3213, 4015, 4905, 7100, 7707
EciI GGCGGA 2 cut(s) 234, 264
Ecl136II GAGCTC 1 cut(s) 6172
Eco130I CCWWGG 8 cut(s) 1255, 1827, 3338, 5447, 6064, 6795, 7094, 7719
Eco147I AGGCCT 1 cut(s) 3678
Eco24I GRGCYC 1 cut(s) 6174
Eco31I GGTCTC 4 cut(s) 313, 1754, 3552, 7290
Eco32I GATATC 2 cut(s) 1794, 6705
Eco53kI GAGCTC 1 cut(s) 6172
Eco57I CTGAAG 5 cut(s) 2604, 3102, 5163, 5775, 7517
Eco72I CACGTG 2 cut(s) 2798, 2831
Eco81I CCTNAGG 1 cut(s) 5455
Eco88I CYCGRG 2 cut(s) 4608, 7275
Eco91I GGTNACC 2 cut(s) 2964, 7082
EcoICRI GAGCTC 1 cut(s) 6172
EcoNI CCTNNNNNAGG 1 cut(s) 5974
EcoO109I RGGNCCY 8 cut(s) 2389, 2680, 3043, 3203, 5451, 6881, 7061, 8009
EcoO65I GGTNACC 2 cut(s) 2964, 7082
EcoRI GAATTC 6 cut(s) 94, 848, 2057, 3252, 4447, 5646
EcoRII CCWGG 7 cut(s) 2479, 2934, 5255, 6436, 6895, 6945, 7659
EcoRV GATATC 2 cut(s) 1794, 6705
EcoT14I CCWWGG 8 cut(s) 1255, 1827, 3338, 5447, 6064, 6795, 7094, 7719
EcoT22I ATGCAT 3 cut(s) 649, 3527, 6110
EcoT38I GRGCYC 1 cut(s) 6174
ErhI CCWWGG 8 cut(s) 1255, 1827, 3338, 5447, 6064, 6795, 7094, 7719
FalI AAGNNNNNCTT 8 cut(s) 1978, 2010, 2577, 2609, 3078, 3110, 7747, 7779
FaqI GGGAC 7 cut(s) 3216, 3676, 4877, 5359, 5571, 6776, 7480
FauI CCCGC 7 cut(s) 257, 324, 4221, 5548, 5617, 5689, 5760
FauNDI CATATG 4 cut(s) 975, 1565, 2989, 4093
FbaI TGATCA 3 cut(s) 1305, 4567, 6045
FblI GTMKAC 6 cut(s) 2613, 2753, 2923, 6091, 6842, 7416
FriOI GRGCYC 1 cut(s) 6174
GlaI GCGC 3 cut(s) 3068, 5202, 6863
GsaI CCCAGC 3 cut(s) 3854, 6435, 7519
GsuI CTGGAG 9 cut(s) 99, 1320, 2502, 4149, 4386, 5325, 5741, 6929, 7626
HaeII RGCGCY 1 cut(s) 5204
HgaI GACGC 3 cut(s) 2599, 5449, 5846
HhaI GCGC 3 cut(s) 3069, 5203, 6864
Hin1I GRCGYC 2 cut(s) 2610, 5460
Hin6I GCGC 3 cut(s) 3067, 5201, 6862
HinP1I GCGC 3 cut(s) 3067, 5201, 6862
HincII GTYRAC 7 cut(s) 1084, 1744, 1845, 2754, 2924, 2941, 6843
HindII GTYRAC 7 cut(s) 1084, 1744, 1845, 2754, 2924, 2941, 6843
HindIII AAGCTT 9 cut(s) 951, 1424, 3387, 4686, 5712, 6020, 6368, 6765, 7256
HpaI GTTAAC 1 cut(s) 2941
Hpy99I CGWCG 5 cut(s) 299, 374, 4464, 5593, 7561
HpyCH4IV ACGT 9 cut(s) 2489, 2797, 2830, 4029, 4459, 5536, 5588, 5995, 6389
HpySE526I ACGT 9 cut(s) 2489, 2797, 2830, 4029, 4459, 5536, 5588, 5995, 6389
Hsp92I GRCGYC 2 cut(s) 2610, 5460
HspAI GCGC 3 cut(s) 3067, 5201, 6862
Kpn2I TCCGGA 1 cut(s) 7071
KpnI GGTACC 1 cut(s) 6462
KroI GCCGGC 1 cut(s) 3855
KroNI GCCGGC 1 cut(s) 3857
Ksp22I TGATCA 3 cut(s) 1305, 4567, 6045
KspAI GTTAAC 1 cut(s) 2941
MaeII ACGT 9 cut(s) 2489, 2797, 2830, 4029, 4459, 5536, 5588, 5995, 6389
MfeI CAATTG 3 cut(s) 4310, 4980, 7997
MhlI GDGCHC 5 cut(s) 1174, 4744, 6001, 6174, 7984
MlsI TGGCCA 1 cut(s) 2894
MluNI TGGCCA 1 cut(s) 2894
MlyI GAGTC 9 cut(s) 290, 401, 2126, 2633, 3163, 5537, 6071, 6233, 7817
MmeI TCCRAC 9 cut(s) 208, 553, 1893, 2246, 3081, 4173, 5244, 6203, 7273
Mox20I TGGCCA 1 cut(s) 2894
Mph1103I ATGCAT 3 cut(s) 649, 3527, 6110
MroI TCCGGA 1 cut(s) 7071
MroNI GCCGGC 1 cut(s) 3855
MroXI GAANNNNTTC 8 cut(s) 647, 990, 3352, 4221, 5389, 6288, 6804, 8019
MscI TGGCCA 1 cut(s) 2894
MslI CAYNNNNRTG 8 cut(s) 1149, 1889, 2334, 2465, 3288, 7446, 7449, 8088
Msp20I TGGCCA 1 cut(s) 2894
MspA1I CMGCKG 5 cut(s) 4228, 4753, 5612, 7519, 7566
MspCI CTTAAG 3 cut(s) 1514, 2810, 7427
MunI CAATTG 3 cut(s) 4310, 4980, 7997
Mva1269I GAATGC 8 cut(s) 647, 649, 1065, 3525, 3880, 6239, 6265, 6273
MvaI CCWGG 7 cut(s) 2481, 2936, 5257, 6438, 6897, 6947, 7661
MvnI CGCG 3 cut(s) 5696, 6638, 6831
NaeI GCCGGC 1 cut(s) 3857
NciI CCSGG 4 cut(s) 301, 3277, 7276, 7277
NdeI CATATG 4 cut(s) 975, 1565, 2989, 4093
NgoMIV GCCGGC 1 cut(s) 3855
NmeAIII GCCGAG 1 cut(s) 1120
NmuCI GTSAC 6 cut(s) 1362, 1938, 2964, 3076, 7015, 7082
NsiI ATGCAT 3 cut(s) 649, 3527, 6110
NspV TTCGAA 5 cut(s) 1984, 3346, 5833, 6187, 7871
PaeI GCATGC 5 cut(s) 808, 1148, 6211, 6308, 7991
PagI TCATGA 3 cut(s) 507, 3283, 7444
PaqCI CACCTGC 2 cut(s) 5476, 7090
PceI AGGCCT 1 cut(s) 3678
PciI ACATGT 3 cut(s) 12, 568, 5560
PcsI WCGNNNNNNNCGW 2 cut(s) 343, 375
PctI GAATGC 8 cut(s) 647, 649, 1065, 3525, 3880, 6239, 6265, 6273
PdiI GCCGGC 1 cut(s) 3857
PdmI GAANNNNTTC 8 cut(s) 647, 990, 3352, 4221, 5389, 6288, 6804, 8019
PfoI TCCNGGA 1 cut(s) 7659
PinAI ACCGGT 1 cut(s) 7086
PleI GAGTC 9 cut(s) 289, 400, 2126, 2632, 3162, 5536, 6071, 6232, 7817
PmaCI CACGTG 2 cut(s) 2798, 2831
PmlI CACGTG 2 cut(s) 2798, 2831
PpsI GAGTC 9 cut(s) 289, 400, 2126, 2632, 3162, 5536, 6071, 6232, 7817
Ppu21I YACGTR 3 cut(s) 2798, 2831, 5537
PpuMI RGGWCCY 5 cut(s) 2680, 3203, 5451, 6881, 8009
PscI ACATGT 3 cut(s) 12, 568, 5560
PshBI ATTAAT 2 cut(s) 3987, 4256
PsiI TTATAA 2 cut(s) 1998, 7746
Psp124BI GAGCTC 1 cut(s) 6174
Psp5II RGGWCCY 5 cut(s) 2680, 3203, 5451, 6881, 8009
Psp6I CCWGG 7 cut(s) 2479, 2934, 5255, 6436, 6895, 6945, 7659
PspCI CACGTG 2 cut(s) 2798, 2831
PspEI GGTNACC 2 cut(s) 2964, 7082
PspFI CCCAGC 3 cut(s) 3850, 6431, 7515
PspGI CCWGG 7 cut(s) 2479, 2934, 5255, 6436, 6895, 6945, 7659
PspPPI RGGWCCY 5 cut(s) 2680, 3203, 5451, 6881, 8009
PstI CTGCAG 6 cut(s) 2212, 3441, 3628, 3730, 6139, 7317
PstNI CAGNNNCTG 4 cut(s) 1582, 3623, 4220, 5491
PvuII CAGCTG 3 cut(s) 4753, 7519, 7566
RseI CAYNNNNRTG 8 cut(s) 1149, 1889, 2334, 2465, 3288, 7446, 7449, 8088
SacI GAGCTC 1 cut(s) 6174
SalI GTCGAC 3 cut(s) 2752, 2922, 6841
ScaI AGTACT 4 cut(s) 2419, 5354, 5752, 6804
SchI GAGTC 9 cut(s) 290, 401, 2126, 2633, 3163, 5537, 6071, 6233, 7817
SduI GDGCHC 5 cut(s) 1174, 4744, 6001, 6174, 7984
SfuI TTCGAA 5 cut(s) 1984, 3346, 5833, 6187, 7871
SmaI CCCGGG 1 cut(s) 7277
SmiMI CAYNNNNRTG 8 cut(s) 1149, 1889, 2334, 2465, 3288, 7446, 7449, 8088
SmlI CTYRAG 8 cut(s) 853, 862, 902, 1514, 2810, 5313, 7231, 7427
SmoI CTYRAG 8 cut(s) 853, 862, 902, 1514, 2810, 5313, 7231, 7427
SpeI ACTAGT 1 cut(s) 7019
SphI GCATGC 5 cut(s) 808, 1148, 6211, 6308, 7991
SseBI AGGCCT 1 cut(s) 3678
SspI AATATT 7 cut(s) 420, 885, 1953, 2359, 4940, 6939, 7909
SstI GAGCTC 1 cut(s) 6174
StuI AGGCCT 1 cut(s) 3678
StyI CCWWGG 8 cut(s) 1255, 1827, 3338, 5447, 6064, 6795, 7094, 7719
TaiI ACGT 9 cut(s) 2492, 2800, 2833, 4032, 4462, 5539, 5591, 5998, 6392
TaqII GACCGA 2 cut(s) 295, 3534
TauI GCSGC 5 cut(s) 272, 5983, 6638, 6989, 7241
TseFI GTSAC 6 cut(s) 1362, 1938, 2964, 3076, 7015, 7082
Tsp45I GTSAC 6 cut(s) 1362, 1938, 2964, 3076, 7015, 7082
TspGWI ACGGA 7 cut(s) 283, 2943, 3704, 3797, 4032, 5109, 7641
TspMI CCCGGG 1 cut(s) 7275
Vha464I CTTAAG 3 cut(s) 1514, 2810, 7427
VneI GTGCAC 2 cut(s) 1170, 5997
VspI ATTAAT 2 cut(s) 3987, 4256
XagI CCTNNNNNAGG 1 cut(s) 5974
XbaI TCTAGA 1 cut(s) 3011
XcmI CCANNNNNNNNNTGG 1 cut(s) 2262
XmaI CCCGGG 1 cut(s) 7275
XmaJI CCTAGG 1 cut(s) 3338
XmiI GTMKAC 6 cut(s) 2613, 2753, 2923, 6091, 6842, 7416
XmnI GAANNNNTTC 8 cut(s) 647, 990, 3352, 4221, 5389, 6288, 6804, 8019
ZrmI AGTACT 4 cut(s) 2419, 5354, 5752, 6804
Zsp2I ATGCAT 3 cut(s) 649, 3527, 6110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.