MD12G1252000.v1.1

Translational activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004626
Physical Location & Seq
Reverse (-)
32125630 .. 32146694
21065 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1252000.v1.1.491

Sequence Viewer

Length: 7881 bp
ATGGCGACTCCCACTGAGTCTCTCCTCTCCATCGCCGGGTCGGTCTCCACCAACTCCACCAAGAAACGGGTTCGGATCTTCCGTGATGAAATTCCCGCCGTCCTCATCAACTCCGAAATTGGTACTGAGTCTGTACTGCCGCTGATAGATATAATCTTTCAAACATTGTACATTTACGATGATCGTGGGTCAAGGAATGCTGTTGATGACATCATTACAAAAGGTTTACAAGAGGTTTCCTTTATGAAAAGTTTTGCGGCAGCCCTTGTTCAGGTCATGGAAAAACAAGCGAAGCTGCAATCACATGTTGGCTGTGATCGACTTCTCCAGTGGTCATGCCTTCTATTGAGTAAGAGTAAGTTTGCCACAGTCTCAAAGAATGCATTGTGTAGAGTTGCCACAGTGCAGGCATTGTTACTCAATCTTGTCATGCAAAGATCTTTCCGTGAGCGAAGGGCTTGCAAGAAGACATTTTGTCGTTTATTTTCTCAGTCACCGGACGTCTACAAAATGTATATAGAGGAACTCAAGGATGCCCGTATCCCATACAAAGATGGTTCTGAATTGATTTGGTTGTTGTTAGAGTACTCAAGTACCTCAAGCAACTCATCTTTGTTTGAACAATGCAAGCCAACGTTTTTGGATATATATCTCAAGGCAATATTAAATGCAAGAGAAAAGCCAGCAAAGGGCCTTAGTGAAGCTTTTCATCCACTTTTTAAACATATGTCGCACGAAGATTTTCAAAATATCGTGCTTCCATCTGCTGTTAAAATGCTGAAACGCAACCCTGAGATTGTATTGGAGTCGGTTGGGATTCTGTTGAAATCAGTCAACCTTGATTTAAGCAAGTATGCTGTCGAAATTCTTTTAGTGATATTGCCGCAGGCTCGGCTTGCGGATGAAGGCAGAAGGGTTGTGGCACTGGCAGTTATACGATGTTTGAGCCAAAAGTCAAGTAATCCAGATACTTTAGAAGCAATGTTCAATGCTGTCAAATCTGTAATTGGAGGCTCAGATGGAAGACTTACGCTTCCATATCAGAGAATTGGCATGATTAACGCACTGCAAGAACTGTGTAATGCTCCAGATGGGAAACACCTTAACAGCCTGTCACAAACATTATGCAGTTTCCTTCTATCTTGTTATAAGGATGATGGAAATGAGGAGGTGAAACTTGCAATTTTGTCAGCTCTGGGTTCATGGGCAGCAAGATCTGCAGATGCTGTTCAATCAAATTTAGTCTCGTTTTTCTCTTCTGGTATTAAGGAGAAAGAAGCTCTAAGAAGAGGGCATCTTCGTTGTCTACGAGCCATATGCAAAAATACAGATGCTGTTTTCCGGATATCATCATTACTGGAACCTCTCATCCAACTTGTCAAAACTGGTTTTACCAAAGTAGCTCAGCGTTTGGATGGTATATATGCCTTACTATTAGTTGGGAAGATTGCTGCTGTTGACATCAAAGCAGAGGAGATTGTTGTGAAGGACAAGATCTGGTATTTAATATCTCAAAATGAGCCTTCACTTGTACCAGTATCCATGGCTTCAAAATTGTCGACTGAGGATTGCATGGCATGTGTTGATCTTCTTGAAATTATGCTGGTGGAACATCTGCGGAGGGTAAAAGACTGCTTTTCAGTCAGATTACTTTCACAGTTAATAATCTTTTTCATATGCCACCCATGCTGGGAAGTTCGAAGGATGACTTATGATGCAACCAGAAGGATAGTTTCTGCTGCTCCGCAATTGACTGAACCTCTTTTAGTTGAATTCACGAATTTCATGTCTGTAGTTGCAGAGAAACTTCATATTTCAAAGTCAAGTGAAACAGATAATTCGTTGGACACTCAAGTGCCATTCCTTCCGTCAGTTGAGGTTTCAGTAAAGGCTTTAATTGTCATATCATCAGTAGCTCTCCCTGCAGCTCCAAGTGCAGCTACGCGAGTTTTGTTCTGCGCTCATCATCCATACTTGGTCGGAACTGCAAAGAGAGATGCAGTTTGGAAGAGGCTACAAAAATGTATGCATGCATGTGGTTTTGACATCATTAGCAACATTTTGGCCGATGTGGAGAATTTGTTCAAGGGCCTACTGGTGCCGATGTTGTTGTCCAGCACTAATCCATTTGAGCAGCAAGCAGCAGTTTCCTCGTTATCCAGTTTGATGTCCATTGCACCTGGAGAAACCTATGCGGAGTTTGAAAAGCACCTGAAAAATCTTCCATATCGTTTTTCACATGACACACTTTCAGAAAATGATATTCGGATTTTTCATACCCCTGAAGGCTTGCTTTCCAGTGAGCAAGGTGTCTACATTGCAGAGTCTGTTGCTGCTAAGAACATGAAACAAGCAAAGGGCCGTTTTCGGATGTATGAGGACCTAGATGACACGGATCAAGGTGGTTCTAATCATTCTGCGAAAGTTGAGCAAACCAATAGTTCTGTGGGTAGAAGGGAAACTGGGAAGTCTGCAAAGAAACCTGATAAGGGAAAGACTGCAAAGGAGGAAGCACGTGAGGTGCAACTTAGGGAGGAGGCATCCATACGCGAGAAGGTCCGAGAAATACAGAAAAATTTGTCTTCCATACTTACAGCTCTTGGGGAAATGGCAATTGCTAATCCTATTTTCGCACACAGTCAACTTCCTTCCCTGGTTAACTATGTTGATCCATTGCTAAGGTCACCGATAGTCAGTGACGTGGCATATGAAACTGTGGTGAAACTCTCTAGATGCACTGCTCCACCTCTTTGTAATTGGGCCCTTGACATTGCTACAGCTTTGCGCTTAGTTGTGACTGAAGAAGTTCGTCTTGTATTGGATATGATTCCATCGGTTGGTGAAGGGGAAGCCAATGAAAGACCATCTTTGAGTCTATTTGAGCGAATAATTAATGGCCTTTCTGTATCTTGCAAAACGGGACCTCTTCCTGTAGATTCTTTTACCTTTGTGTTCCCTATAATGGAGCGAATTCTGTTATGCTCTAAGAAGACAGGACTTCACAATGATGTTCTTCAGATTCTGTATCTGCACATGGACCCCCTGTTACCTCTTCCAAGGCTTCGAATGATTTCAGTTCTTTATCATGTACTTGGTGTTGTTCCAGCTTATCAAGCCTCTGTTGGACCTGCGTTGAATGAGTTGTGTTTGGGCCTGCAACCAGATGAAGTGGCAGCAGCTCTGTATGGTGTTTATGCAAAAGATGTTCATGTTAGAATGGCCTGCCTTAGTGCTGTCAAATGTATTCCTGCTGTTGCCAGTCGGTCTCTTCCTCAGAACGTTGAAGTCGCTACAGGCATTTGGGTTGCCTTACATGACCCAGAAAAGTCCGTTGCAGAAGCAGCAGAGGATTTATGGGATCGGTATGGGCATGACTTTGGGACAGACTATTCAGGCCTTTTCAAAGCACTTTCGCATATCAATTATAATGTCCGTTTTGCTGCTGCGGAGGCATTAGCTGCTGCTTTGGATGAATGTCCCGATTCTATACAGGAGTCTCTTTCTACTTTATTTTCTCTGTATATCCGTGATGCTGGTTTGACCGAGGAGAATGTTGATGCTGGTTGGCTGGGTCGACAAGGTGTAGCCTTGGCATTACATTCTTCTGCTGATGTACTCAGAACAAAAGACCTTCCTGTTGTTATGACATTTCTTATTTCAAGAGCACTGGCTGATCCTAATACTGATGTTAGGGGAAGAATGATCAATGCTGGTATTATGATTATTGATAAGCATGGCAGGGATAATGTTTCCTTGCTATTTCCCATCTTTGAGAACTACTTGAACAAAAAGGCATCAGATGAAGAGAAATATGATTTGGTACGTGAGGGGGTTGTTATTTTCACTGGAGCTTTGGCAAAACATTTGGCAAAGGATGATCCAAAAGTTCATGTTGTTGTCGAGAAGTTGTTGGATGTGCTAAACACTCCATCAGAAGCTGTTCAACGGGCTGTATCTGCATGCTTGTCTCCACTAATGCAATCAAAGCAAGATGATGGACCAGCTCTTGTCTCGAGGCTTTTGGATAAACTGATGAAGAGTGACAAATATGGGGAACGCCGTGGAGCTGCTTTCGGGCTTGCTGGAGTGGTCAAGGGATTCGGTATTTCTTGCTTGAAGAAATATGGGATTGTAGCTCGACTACAAGAAGGACTTGTAGATAGGAATTCAGCAAAATGTCGTGAAGGAGCTTTACTCGGATTTGAGTGTCTCTGTGAATCACTCGGAAGACTATTTGAGCCGTATGTAATTAAAATGTTGCCCCTACTGTTGGTTTCATTCTCTGATCAAGTTGTTGCAGTTCGTGAAGGTGCCGAGTGTGCAGCTCGTGCAATGATGTCTCAACTTAGTGCTCAAGGAGTTAAACTGGTTCTTCCATCTCTTTTGAAGGGTCTTGAAGATAAAGCTTGGCGAACAAAGCAAAGTAGTGTCCAACTGCTTGGGGCTATGGCCTATTGTGCTCCTCAACAGCTGTCTCAGTGTCTCCCTAAGATTGTTCCAAAATTGACTGAGGTTTTAACAGATACACATCCTAAAGTCCAGTCAGCTGGACAAACGGCACTTCAACAGGTTGGGAGTGTAATAAAGAATCCTGAAATAGCTTCCCTTGTTCCTACTCTTCTACTGGGTCTTTCAGATCCAAATGACCATACAAAGTACTCTCTTGATGTTCTCTTACAGACCACTTTTATTAATACAATTGATGCTCCCTCACTGGCACTGTTGGTGCCAATAGTACATAGAGGGCTACGGGAAAGGAGTGCTGAAACTAAAAAGAAAGCAGCACAAATAGTTGGCAATATGTGTTCATTAGTTACAGAACCTAAGGATATGATTCCATATATTGACTTACTTCTTCCTGAAGTGAAAAAGGTCCTTGTGGATCCAATTCCAGAAGTTCGGTCAGTTGCAGCAAGGGCCCTTGGATCCCTTATAAGGGGAATGGGCGAAGATCATTTCCCAGATCTCGTTCCATGGTTGTTTGATACACTTAAATCAGATAATAGTAATGTTGAGCGCTCTGGAGCAGCTCAAGGGTTGAGCGAGGTATTAGCTGCACTTGGAACTGAGTACTTTGAGCTTGCACTTCCTGATATTATCCGAAACTGTTCTCATCAAAAAGCATCTGTCCGTGATGGATATTTAACATTATTTAAGTATTTACCAAGGTCATTGGGCGTTCAATTCCAGAACTATTTACAGCAGGTGCTGCCTGCTATTTTAGATGGTCTTGCTGATGAAAATGAGTCTGTACGTGAGGCAGCTCTAGGTGCAGGGCATATTCTGGTTGAGCATTATGCAACAACGTCTTTACCTTTGCTCCTTCCGGTTGTTGAAGATGGTATCTTTAATGATAGTTGGCGGATTCGGCAAAGCTCTGTGGAATTATTGGGTGATCTCTTATTTAAGGTTGCTGGAACTTCTGGGAAAGCTTTACTTGAGGGTGGCAGTGATGATGAAGGTGCTAGCACTGAAGCACAGGGTCGGGCTATCATTGAGGTTCTGGGAAGGGAAAAGCGGGATGAAGTTCTTGCTGCATTATATATGGTTCGAACAGATGTCAGCTTATCAGTTCGTCAGGCTGCGCTGCATGTGTGGAAAACTATTGTGGCAAATACTCCGAAGACTCTAAAGGAAATAATGCCAGTTTTAATGAATACTCTAATTGCCTCTCTTGCTTCATCGTCCTCTGAAAGGCGGCAGGTTGCTGGGCGATCACTGGGTGAGCTTGTCAGGAAACTTGGTGAAAGAGTACTTCCATGGATCATCCCAATTTTATCCCAAGGGTTAAAGGATTCTGACGTTGGCAGAAGACAAGGAGTATGCATTGGTCTGAGTGAAGTAATGGCAAGTGCTGGCAAAAATCAGTTGTTGAGTTTCATGGATGAACTCATACCGACCATTCGAACTGCTCTTTCGGATAGCATGCCCGAGGTTCGTGAGGCTGCTGGGTTAGCATTCAACACTCTCTACAAGAGTGCTGGAATGCAAGCAATCGATGAAATTGTTCCCACTCTTTTGCATGCTTTGGAGGATGATGAAACTTCTGATACTGCTCTTGATGGCCTCAAACAAATCTTAAGCGTCAGGATTTCTGCTGTTTTGCCCCACATCTTACCCAAGCTGGTCCACCTGCCACTTACTGCGTTTAATGCACATGCTCTTGGAGCGGTAGCTGAGGTGGCTGGACCTGGTCTTAACTCTCATCTTGGTACTGTTCTTCCTGCTTTACTTTCTGCAATGAGTGCTGATGAGAAGGAAGTTCAAAAGTTGGCAAAGGAGGCTGCTGAGACTGTGGTCTTAGTCATAGATGAGGAAGGTGTTGAATCTCTTTTATCTGAGCTTGTTCGAGCTGTCAGTGATAGTCAGGCTTCAATTAGAAGAAGTTCTTCGTATCTTATTGGCTATTTCTTCAAAAGCAGTAAATTATATTTGGTTGATGAAGCTCCAAACGTGATATCTACCCTGATTGTGTTGCTCAGTGATTCAGATTCAGCTACTGTGGCGGCTTCTTGGGAAGCTTTGTCAAGAGTTGTAAGTTCAGTTCCCAAGGAGGTACTTCCGTCATATATTAAATTAGTACGAGATGCTGTATCTACTTCCCGGGATAAGGAGCGTAGAAAGAAGAAGGGAGGACCAATTCTCATTCCTGGGTTTTGTTTACCAAAAGCACTTCAACCTTTGCTTCCAATATTTATCCAAGGTTTGACAAGTGGATCTGCTGAACTAAGAGAGCAAGCTGCTCTAGGTCTCGGTGAACTTATCGAAGTGACTAGCGAACAGGCACTGAAAGAGTTTGTCATCCCAATTACAGGGCCTCTTATCCGGATAATAGGTGATCGGTTCCCTTGGCAGGTGAAGAGTGCGATTTTGTCTACCTTGACAATCATGATAAGAAAGGGTGGAATGGCATTGAAACCTTTTCTTCCTCAGCTTCAAACAACATTTGTAAAGTGTCTACAGGATAGCACAAGAATCGTTCGTTCAAGTGCTGCCCTTGCACTCGGAAAGCTTAGTGCTCTCAGTACCCGGGTTGACCCTTTAGTTGGAGACCTCCTTTCCAGTTTGCAGGCTCCGGATAGTGGGGTTCGGGAGGCATGTTTGTCTGCTCTCGAAGGTGTCGTAAAGCATGCTGGAAAAAGTGTGAGCAGTGCTGTTAGAACTCGTCTCTACTCGCACCTTAAGGACTTGATTCATCATGATGATGACCAGGTTCGGATATCTGCTGCAAGCATTTTGGGCATCACATCCCAGTACCTAGAAGATGATCAACTGACGGAGCTGCTTCAGGAACTCTCTGATTTGCCATCATCTCTTAGCTTGTCTGCCAGGCATGGCTCCGTACTCACAATATCATCCATGCTCAGGCACAACCCTTCCACTATTTGTTTGTCTCCAGAGTTTCCGTCTATCTTGGGCCGGCTTAAAGACGCCTTGACTGATGAGAAGTTCCCCCTGCGTGAAACATCGACCAAGGCATTTGGGAGACTTCTGATTCACAAGCTCCGAAGTGATCCTTCTAATAAATCACTCCATTTGGAAATAATATCGTCCTTAGTGTCAGCTTTGCGCGATGATTCTAGTGAAGTTAGAAGAAAGGCATTATCTGCTATAAAACGAGGTTCCAAGGAAAATGCTTCAGTTATTTTGGCTCATATCAATATTATTGGACCGGCTCTTGCTGAATGCTTGAAAGATGGAAGTACTCCCGTGAGACTTGCAGCTGAAAGATGTGCTCTGCATGCTTTCCAATTGTCAAAGGGTCCTGAAAATATTCAAGCTGCACAAAAATTCATCACAGGCTTGGATGCCAGAAGAATGTCGAAGCTTCCTGAAAATAGCGATGATAGTGAAGATGGTGAGGACGCGGCAAGTGGCTGA

Protein Analysis

2627

Amino Acids

286.87

Weight (kDa)

6.55

Isoelectric Point (pI)

43.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GNC1_N PF24993 210 - 343 1.6e-42 Stalled ribosome sensor GCN1 N-terminal
HEAT_EF3_N PF24987 1353 - 1520 1.9e-46 EF3 N-terminal HEAT repeat
HEAT_GCN1 PF23271 1403 - 1540 1.6e-10 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_GNC1 PF24984 1558 - 1644 6.9e-27 Elongation factor 3/GNC1 HEAT repeat
TOR1L1_N PF24714 1562 - 1756 2.5e-06 TORTIFOLIA1 N-terminal
HEAT_GCN1 PF23271 1632 - 1779 1.5e-10 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_N PF24987 1723 - 1802 1.3e-07 EF3 N-terminal HEAT repeat
HEAT_GCN1 PF23271 1875 - 2013 1.4e-06 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_N PF24987 1885 - 1983 6.1e-07 EF3 N-terminal HEAT repeat
HEAT_GCN1 PF23271 2034 - 2223 9.5e-53 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_GCN1_C_2 PF25801 2346 - 2597 5.6e-29 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_GCN1_C PF25786 2444 - 2598 1.5e-72 GCN1-like, C-terminal HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1149, 3426, 4934
AarI CACCTGC 3 cut(s) 5194, 6141, 6844
AatII GACGTC 1 cut(s) 504
Acc36I ACCTGC 5 cut(s) 3136, 5194, 5692, 6141, 6844
AccB1I GGYRCC 3 cut(s) 2098, 4310, 4726
AccB7I CCANNNNNTGG 1 cut(s) 2837
AccBSI CCGCTC 1 cut(s) 6170
AccI GTMKAC 7 cut(s) 504, 1306, 1559, 2313, 3574, 6875, 6958
AccII CGCG 4 cut(s) 1945, 2550, 7572, 7868
AccIII TCCGGA 3 cut(s) 1341, 6825, 7075
AclI AACGTT 2 cut(s) 635, 3279
AcoI YGGCCR 1 cut(s) 2064
AcuI CTGAAG 7 cut(s) 2304, 2820, 2999, 4881, 5493, 7271, 7623
AcvI CACGTG 1 cut(s) 2516
AcyI GRCGYC 2 cut(s) 501, 7431
AdeI CACNNNGTG 1 cut(s) 5723
AfeI AGCGCT 1 cut(s) 5018
AflII CTTAAG 2 cut(s) 6079, 7181
AflIII ACRYGT 1 cut(s) 304
AhdI GACNNNNNGTC 1 cut(s) 474
AjiI CACGTC 1 cut(s) 2701
AjnI CCWGG 6 cut(s) 2179, 2652, 6190, 6649, 7209, 7328
AjuI GAANNNNNNNTTGG 2 cut(s) 3800, 3832
AleI CACNNNNGTG 1 cut(s) 1853
AloI GAACNNNNNNTCC 4 cut(s) 1353, 1385, 2066, 2098
Alw21I GWGCWC 5 cut(s) 3667, 4354, 4462, 7021, 7738
AlwNI CAGNNNCTG 8 cut(s) 1226, 1334, 2327, 3022, 3938, 5209, 6500, 6787
Ama87I CYCGRG 4 cut(s) 4012, 5930, 6603, 7029
Aor13HI TCCGGA 3 cut(s) 1341, 6825, 7075
Aor51HI AGCGCT 1 cut(s) 5018
ApaI GGGCCC 2 cut(s) 2764, 4921
ArsI GACNNNNNNTTYG 2 cut(s) 1772, 1804
AseI ATTAAT 2 cut(s) 2892, 4692
AspLEI GCGC 5 cut(s) 1961, 2787, 5019, 5587, 7572
AsuC2I CCSGG 5 cut(s) 37, 6604, 6605, 7030, 7031
AsuII TTCGAA 4 cut(s) 1699, 3064, 5551, 5905
AsuNHI GCTAGC 1 cut(s) 5465
AvaI CYCGRG 4 cut(s) 4012, 5930, 6603, 7029
AxyI CCTNAGG 1 cut(s) 4824
BaeGI GKGCMC 2 cut(s) 2764, 4921
BaeI ACNNNNGTAYC 4 cut(s) 577, 610, 6548, 6581
BamHI GGATCC 2 cut(s) 4882, 4925
BanI GGYRCC 3 cut(s) 2098, 4310, 4726
BanII GRGCYC 2 cut(s) 2764, 4921
BarI GAAGNNNNNNTAC 2 cut(s) 4176, 4208
BauI CACGAG 1 cut(s) 4326
BbrPI CACGTG 1 cut(s) 2516
BbsI GAAGAC 7 cut(s) 473, 1030, 2574, 2996, 4234, 5630, 5818
Bbv12I GWGCWC 5 cut(s) 3667, 4354, 4462, 7021, 7738
BbvCI CCTCAGC 2 cut(s) 6177, 6930
BceAI ACGGC 5 cut(s) 83, 2346, 4044, 4225, 4572
BcgI CGANNNNNNTGC 8 cut(s) 441, 475, 1299, 1333, 2133, 2167, 6958, 6992
BciT130I CCWGG 6 cut(s) 2181, 2654, 6192, 6651, 7211, 7330
BciVI GTATCC 2 cut(s) 551, 1549
BclI TGATCA 3 cut(s) 3702, 4285, 7267
BcnI CCSGG 5 cut(s) 37, 6604, 6605, 7030, 7031
BfaI CTAG 8 cut(s) 2384, 2730, 5267, 5466, 6746, 6774, 7259, 7581
BfmI CTRYAG 7 cut(s) 1218, 1791, 1923, 2775, 2931, 3291, 6959
BfoI RGCGCY 1 cut(s) 5020
BfrI CTTAAG 2 cut(s) 6079, 7181
BfuAI ACCTGC 5 cut(s) 3136, 5194, 5692, 6141, 6844
BfuI GTATCC 2 cut(s) 551, 1549
BglII AGATCT 4 cut(s) 437, 1214, 1494, 4963
BlpI GCTNAGC 1 cut(s) 1404
BmcAI AGTACT 5 cut(s) 587, 4658, 5072, 5754, 7705
BmeRI GACNNNNNGTC 1 cut(s) 474
BmeT110I CYCGRG 4 cut(s) 4012, 5930, 6603, 7029
BmgBI CACGTC 1 cut(s) 2701
BmrI ACTGGG 4 cut(s) 2472, 4634, 5729, 7246
BmtI GCTAGC 1 cut(s) 5469
BmuI ACTGGG 4 cut(s) 2472, 4634, 5729, 7246
BoxI GACNNNNGTC 1 cut(s) 6296
BpiI GAAGAC 7 cut(s) 473, 1030, 2574, 2996, 4234, 5630, 5818
BplI GAGNNNNNCTC 2 cut(s) 4179, 4211
BpmI CTGGAG 7 cut(s) 311, 1071, 2202, 3867, 4104, 5043, 7380
Bpu10I CCTNAGC 4 cut(s) 2678, 6177, 6930, 7364
Bpu1102I GCTNAGC 1 cut(s) 1404
Bpu14I TTCGAA 4 cut(s) 1699, 3064, 5551, 5905
BpuEI CTTGAG 8 cut(s) 512, 574, 583, 638, 1836, 4338, 5016, 5459
BpuMI CCSGG 5 cut(s) 37, 6604, 6605, 7030, 7031
Bsa29I ATCGAT 1 cut(s) 5997
BsaAI YACGTR 3 cut(s) 2516, 3824, 5255
BsaBI GATNNNNATC 2 cut(s) 648, 4527
BsaHI GRCGYC 2 cut(s) 501, 7431
BsaI GGTCTC 4 cut(s) 49, 3270, 6755, 7044
BsaWI WCCGGW 5 cut(s) 496, 1341, 5326, 6825, 7075
BsaXI ACNNNNNCTCC 4 cut(s) 2066, 2096, 5304, 5334
Bse118I RCCGGY 2 cut(s) 7419, 7672
Bse21I CCTNAGG 1 cut(s) 4824
Bse3DI GCAATG 7 cut(s) 987, 2172, 2316, 2672, 2769, 4338, 6246
Bse8I GATNNNNATC 2 cut(s) 648, 4527
BseAI TCCGGA 3 cut(s) 1341, 6825, 7075
BseBI CCWGG 6 cut(s) 2181, 2654, 6192, 6651, 7211, 7330
BseCI ATCGAT 1 cut(s) 5997
BseJI GATNNNNATC 2 cut(s) 648, 4527
BseMI GCAATG 7 cut(s) 987, 2172, 2316, 2672, 2769, 4338, 6246
BseRI GAGGAG 6 cut(s) 14, 1181, 1487, 2549, 3560, 4452
BseSI GKGCMC 2 cut(s) 2764, 4921
BseYI CCCAGC 4 cut(s) 1689, 3568, 5708, 5948
BsgI GTGCAG 7 cut(s) 425, 1956, 3014, 4341, 5040, 5292, 7767
Bsh1236I CGCG 4 cut(s) 1945, 2550, 7572, 7868
BshNI GGYRCC 3 cut(s) 2098, 4310, 4726
BshVI ATCGAT 1 cut(s) 5997
BsiHKAI GWGCWC 5 cut(s) 3667, 4354, 4462, 7021, 7738
BsiHKCI CYCGRG 4 cut(s) 4012, 5930, 6603, 7029
BslFI GGGAC 3 cut(s) 2934, 3394, 3462
BsmBI CGTCTC 1 cut(s) 7172
BsmFI GGGAC 3 cut(s) 2934, 3394, 3462
BsmI GAATGC 5 cut(s) 202, 385, 5957, 5991, 7691
Bso31I GGTCTC 4 cut(s) 49, 3270, 6755, 7044
BsoBI CYCGRG 4 cut(s) 4012, 5930, 6603, 7029
Bsp119I TTCGAA 4 cut(s) 1699, 3064, 5551, 5905
Bsp120I GGGCCC 2 cut(s) 2760, 4917
Bsp1286I GDGCHC 7 cut(s) 2764, 3667, 4354, 4462, 4921, 7021, 7738
Bsp13I TCCGGA 3 cut(s) 1341, 6825, 7075
Bsp1407I TGTACA 1 cut(s) 168
Bsp1720I GCTNAGC 1 cut(s) 1404
Bsp19I CCATGG 3 cut(s) 1542, 4973, 5759
BspDI ATCGAT 1 cut(s) 5997
BspEI TCCGGA 3 cut(s) 1341, 6825, 7075
BspFNI CGCG 4 cut(s) 1945, 2550, 7572, 7868
BspHI TCATGA 2 cut(s) 6888, 7198
BspMAI CTGCAG 2 cut(s) 1222, 1927
BspMI ACCTGC 5 cut(s) 3136, 5194, 5692, 6141, 6844
BspOI GCTAGC 1 cut(s) 5469
BspT104I TTCGAA 4 cut(s) 1699, 3064, 5551, 5905
BspT107I GGYRCC 3 cut(s) 2098, 4310, 4726
BspTI CTTAAG 2 cut(s) 6079, 7181
BspTNI GGTCTC 4 cut(s) 49, 3270, 6755, 7044
BsrBI CCGCTC 1 cut(s) 6170
BsrDI GCAATG 7 cut(s) 987, 2172, 2316, 2672, 2769, 4338, 6246
BsrFI RCCGGY 2 cut(s) 7419, 7672
BsrGI TGTACA 1 cut(s) 168
BssAI RCCGGY 2 cut(s) 7419, 7672
BssNI GRCGYC 2 cut(s) 501, 7431
BssSI CACGAG 1 cut(s) 4326
Bst2BI CACGAG 1 cut(s) 4326
Bst2UI CCWGG 6 cut(s) 2181, 2654, 6192, 6651, 7211, 7330
BstACI GRCGYC 2 cut(s) 501, 7431
BstAFI CTTAAG 2 cut(s) 6079, 7181
BstAPI GCANNNNNTGC 6 cut(s) 1217, 3458, 4328, 5209, 6245, 7607
BstAUI TGTACA 1 cut(s) 168
BstBAI YACGTR 3 cut(s) 2516, 3824, 5255
BstBI TTCGAA 4 cut(s) 1699, 3064, 5551, 5905
BstDSI CCRYGG 4 cut(s) 1542, 4060, 4973, 5759
BstEII GGTNACC 1 cut(s) 2682
BstENI CCTNNNNNAGG 2 cut(s) 269, 5692
BstFNI CGCG 4 cut(s) 1945, 2550, 7572, 7868
BstH2I RGCGCY 1 cut(s) 5020
BstHHI GCGC 5 cut(s) 1961, 2787, 5019, 5587, 7572
BstNI CCWGG 6 cut(s) 2181, 2654, 6192, 6651, 7211, 7330
BstPAI GACNNNNGTC 1 cut(s) 6296
BstPI GGTNACC 1 cut(s) 2682
BstSFI CTRYAG 7 cut(s) 1218, 1791, 1923, 2775, 2931, 3291, 6959
BstSLI GKGCMC 2 cut(s) 2764, 4921
BstUI CGCG 4 cut(s) 1945, 2550, 7572, 7868
BstV2I GAAGAC 7 cut(s) 473, 1030, 2574, 2996, 4234, 5630, 5818
BstX2I RGATCY 9 cut(s) 75, 437, 1214, 1494, 4636, 4882, 4925, 4963, 6716
BstXI CCANNNNNNTGG 2 cut(s) 4439, 4547
BstYI RGATCY 9 cut(s) 75, 437, 1214, 1494, 4636, 4882, 4925, 4963, 6716
Bsu15I ATCGAT 1 cut(s) 5997
Bsu36I CCTNAGG 1 cut(s) 4824
BsuI GTATCC 2 cut(s) 551, 1549
BsuTUI ATCGAT 1 cut(s) 5997
BtgI CCRYGG 4 cut(s) 1542, 4060, 4973, 5759
BtgZI GCGATG 3 cut(s) 16, 7587, 7857
BtrI CACGTC 1 cut(s) 2701
BtsI GCAGTG 4 cut(s) 1064, 2736, 5455, 7156
BveI ACCTGC 5 cut(s) 3136, 5194, 5692, 6141, 6844
CaiI CAGNNNCTG 8 cut(s) 1226, 1334, 2327, 3022, 3938, 5209, 6500, 6787
CciI TCATGA 2 cut(s) 6888, 7198
CfoI GCGC 5 cut(s) 1961, 2787, 5019, 5587, 7572
Cfr10I RCCGGY 2 cut(s) 7419, 7672
Cfr9I CCCGGG 2 cut(s) 6603, 7029
ClaI ATCGAT 1 cut(s) 5997
CseI GACGC 3 cut(s) 6073, 7439, 7874
CsiI ACCWGGT 2 cut(s) 6190, 7209
DraI TTTAAA 1 cut(s) 721
DraIII CACNNNGTG 1 cut(s) 5723
DriI GACNNNNNGTC 1 cut(s) 474
EaeI YGGCCR 1 cut(s) 2064
Eam1105I GACNNNNNGTC 1 cut(s) 474
EciI GGCGGA 1 cut(s) 5377
Eco147I AGGCCT 1 cut(s) 3396
Eco24I GRGCYC 2 cut(s) 2764, 4921
Eco31I GGTCTC 4 cut(s) 49, 3270, 6755, 7044
Eco32I GATATC 3 cut(s) 1347, 6459, 7221
Eco47III AGCGCT 1 cut(s) 5018
Eco57I CTGAAG 7 cut(s) 2304, 2820, 2999, 4881, 5493, 7271, 7623
Eco72I CACGTG 1 cut(s) 2516
Eco81I CCTNAGG 1 cut(s) 4824
Eco88I CYCGRG 4 cut(s) 4012, 5930, 6603, 7029
Eco91I GGTNACC 1 cut(s) 2682
EcoNI CCTNNNNNAGG 2 cut(s) 269, 5692
EcoO65I GGTNACC 1 cut(s) 2682
EcoRI GAATTC 3 cut(s) 1772, 2970, 4165
EcoRII CCWGG 6 cut(s) 2179, 2652, 6190, 6649, 7209, 7328
EcoRV GATATC 3 cut(s) 1347, 6459, 7221
EcoT22I ATGCAT 4 cut(s) 385, 2031, 2035, 5828
EcoT38I GRGCYC 2 cut(s) 2764, 4921
Esp3I CGTCTC 1 cut(s) 7172
FaqI GGGAC 3 cut(s) 2934, 3394, 3462
FauI CCCGC 2 cut(s) 103, 5511
FauNDI CATATG 4 cut(s) 726, 1316, 1676, 2707
FbaI TGATCA 3 cut(s) 3702, 4285, 7267
FblI GTMKAC 7 cut(s) 504, 1306, 1559, 2313, 3574, 6875, 6958
FriOI GRGCYC 2 cut(s) 2764, 4921
FspBI CTAG 8 cut(s) 2384, 2730, 5267, 5466, 6746, 6774, 7259, 7581
GlaI GCGC 5 cut(s) 1960, 2786, 5018, 5586, 7571
GsaI CCCAGC 4 cut(s) 1693, 3572, 5712, 5952
GsuI CTGGAG 7 cut(s) 311, 1071, 2202, 3867, 4104, 5043, 7380
HaeII RGCGCY 1 cut(s) 5020
HgaI GACGC 3 cut(s) 6073, 7439, 7874
HhaI GCGC 5 cut(s) 1961, 2787, 5019, 5587, 7572
Hin1I GRCGYC 2 cut(s) 501, 7431
Hin6I GCGC 5 cut(s) 1959, 2785, 5017, 5585, 7570
HinP1I GCGC 5 cut(s) 1959, 2785, 5017, 5585, 7570
HincII GTYRAC 7 cut(s) 835, 1459, 1560, 2642, 2659, 3575, 7036
HindII GTYRAC 7 cut(s) 835, 1459, 1560, 2642, 2659, 3575, 7036
HindIII AAGCTT 6 cut(s) 702, 4404, 5430, 6519, 7010, 7826
HpaI GTTAAC 1 cut(s) 2659
Hsp92I GRCGYC 2 cut(s) 501, 7431
HspAI GCGC 5 cut(s) 1959, 2785, 5017, 5585, 7570
Kpn2I TCCGGA 3 cut(s) 1341, 6825, 7075
KroI GCCGGC 1 cut(s) 7419
KroNI GCCGGC 1 cut(s) 7421
Ksp22I TGATCA 3 cut(s) 3702, 4285, 7267
KspAI GTTAAC 1 cut(s) 2659
MabI ACCWGGT 2 cut(s) 6190, 7209
MaeI CTAG 8 cut(s) 2384, 2730, 5267, 5466, 6746, 6774, 7259, 7581
MbiI CCGCTC 1 cut(s) 6170
MfeI CAATTG 4 cut(s) 1748, 2613, 4698, 7751
MflI RGATCY 9 cut(s) 75, 437, 1214, 1494, 4636, 4882, 4925, 4963, 6716
MhlI GDGCHC 7 cut(s) 2764, 3667, 4354, 4462, 4921, 7021, 7738
MlyI GAGTC 8 cut(s) 26, 137, 815, 2333, 2881, 3503, 5255, 5620
MmeI TCCRAC 7 cut(s) 1396, 1824, 1960, 3103, 3891, 4456, 7027
Mph1103I ATGCAT 4 cut(s) 385, 2031, 2035, 5828
MroI TCCGGA 3 cut(s) 1341, 6825, 7075
MroNI GCCGGC 1 cut(s) 7419
MslI CAYNNNNRTG 5 cut(s) 1853, 2034, 3006, 7200, 7203
MspA1I CMGCKG 4 cut(s) 142, 4471, 4547, 7724
MspCI CTTAAG 2 cut(s) 6079, 7181
MunI CAATTG 4 cut(s) 1748, 2613, 4698, 7751
Mva1269I GAATGC 5 cut(s) 202, 385, 5957, 5991, 7691
MvaI CCWGG 6 cut(s) 2181, 2654, 6192, 6651, 7211, 7330
MvnI CGCG 4 cut(s) 1945, 2550, 7572, 7868
NaeI GCCGGC 1 cut(s) 7421
NciI CCSGG 5 cut(s) 37, 6604, 6605, 7030, 7031
NcoI CCATGG 3 cut(s) 1542, 4973, 5759
NdeI CATATG 4 cut(s) 726, 1316, 1676, 2707
NgoMIV GCCGGC 1 cut(s) 7419
NheI GCTAGC 1 cut(s) 5465
NmeAIII GCCGAG 2 cut(s) 871, 4339
NmuCI GTSAC 7 cut(s) 492, 1113, 2682, 2696, 2794, 4040, 6769
NsiI ATGCAT 4 cut(s) 385, 2031, 2035, 5828
NspV TTCGAA 4 cut(s) 1699, 3064, 5551, 5905
OliI CACNNNNGTG 1 cut(s) 1853
PaeI GCATGC 6 cut(s) 2033, 3963, 5929, 6026, 7133, 7745
PaeR7I CTCGAG 1 cut(s) 4012
PagI TCATGA 2 cut(s) 6888, 7198
PaqCI CACCTGC 3 cut(s) 5194, 6141, 6844
PceI AGGCCT 1 cut(s) 3396
PciI ACATGT 1 cut(s) 304
PcsI WCGNNNNNNNCGW 3 cut(s) 79, 1306, 7119
PctI GAATGC 5 cut(s) 202, 385, 5957, 5991, 7691
PdiI GCCGGC 1 cut(s) 7421
PflFI GACNNNGTC 1 cut(s) 6192
PflMI CCANNNNNTGG 1 cut(s) 2837
PleI GAGTC 8 cut(s) 25, 136, 814, 2332, 2880, 3502, 5254, 5620
PmaCI CACGTG 1 cut(s) 2516
PmlI CACGTG 1 cut(s) 2516
PpsI GAGTC 8 cut(s) 25, 136, 814, 2332, 2880, 3502, 5254, 5620
Ppu21I YACGTR 3 cut(s) 2516, 3824, 5255
PpuMI RGGWCCY 4 cut(s) 2380, 2921, 4873, 7763
PscI ACATGT 1 cut(s) 304
PshAI GACNNNNGTC 1 cut(s) 6296
PshBI ATTAAT 2 cut(s) 2892, 4692
PsiI TTATAA 3 cut(s) 1149, 3426, 4934
Psp1406I AACGTT 2 cut(s) 635, 3279
Psp5II RGGWCCY 4 cut(s) 2380, 2921, 4873, 7763
Psp6I CCWGG 6 cut(s) 2179, 2652, 6190, 6649, 7209, 7328
PspCI CACGTG 1 cut(s) 2516
PspEI GGTNACC 1 cut(s) 2682
PspFI CCCAGC 4 cut(s) 1689, 3568, 5708, 5948
PspGI CCWGG 6 cut(s) 2179, 2652, 6190, 6649, 7209, 7328
PspOMI GGGCCC 2 cut(s) 2760, 4917
PspPPI RGGWCCY 4 cut(s) 2380, 2921, 4873, 7763
PsrI GAACNNNNNNTAC 2 cut(s) 6528, 6560
PstI CTGCAG 2 cut(s) 1222, 1927
PstNI CAGNNNCTG 8 cut(s) 1226, 1334, 2327, 3022, 3938, 5209, 6500, 6787
PsuI RGATCY 9 cut(s) 75, 437, 1214, 1494, 4636, 4882, 4925, 4963, 6716
PsyI GACNNNGTC 1 cut(s) 6192
PvuII CAGCTG 3 cut(s) 4471, 4547, 7724
RseI CAYNNNNRTG 5 cut(s) 1853, 2034, 3006, 7200, 7203
SalI GTCGAC 2 cut(s) 1558, 3573
ScaI AGTACT 5 cut(s) 587, 4658, 5072, 5754, 7705
SchI GAGTC 8 cut(s) 26, 137, 815, 2333, 2881, 3503, 5255, 5620
SduI GDGCHC 7 cut(s) 2764, 3667, 4354, 4462, 4921, 7021, 7738
SexAI ACCWGGT 2 cut(s) 6190, 7209
SfcI CTRYAG 7 cut(s) 1218, 1791, 1923, 2775, 2931, 3291, 6959
Sfr274I CTCGAG 1 cut(s) 4012
SfuI TTCGAA 4 cut(s) 1699, 3064, 5551, 5905
SlaI CTCGAG 1 cut(s) 4012
SmaI CCCGGG 2 cut(s) 6605, 7031
SmiMI CAYNNNNRTG 5 cut(s) 1853, 2034, 3006, 7200, 7203
SphI GCATGC 6 cut(s) 2033, 3963, 5929, 6026, 7133, 7745
SseBI AGGCCT 1 cut(s) 3396
SspI AATATT 4 cut(s) 663, 6693, 7663, 7774
SspMI CTAG 8 cut(s) 2384, 2730, 5267, 5466, 6746, 6774, 7259, 7581
StuI AGGCCT 1 cut(s) 3396
TaqII GACCGA 4 cut(s) 31, 3252, 3557, 4890
TauI GCSGC 6 cut(s) 142, 260, 886, 5701, 6509, 7871
TseFI GTSAC 7 cut(s) 492, 1113, 2682, 2696, 2794, 4040, 6769
Tsp45I GTSAC 7 cut(s) 492, 1113, 2682, 2696, 2794, 4040, 6769
TspMI CCCGGG 2 cut(s) 6603, 7029
Tth111I GACNNNGTC 1 cut(s) 6192
Van91I CCANNNNNTGG 1 cut(s) 2837
Vha464I CTTAAG 2 cut(s) 6079, 7181
VspI ATTAAT 2 cut(s) 2892, 4692
XagI CCTNNNNNAGG 2 cut(s) 269, 5692
XbaI TCTAGA 1 cut(s) 2729
XcmI CCANNNNNNNNNTGG 1 cut(s) 2443
XhoI CTCGAG 1 cut(s) 4012
XmaI CCCGGG 2 cut(s) 6603, 7029
XmiI GTMKAC 7 cut(s) 504, 1306, 1559, 2313, 3574, 6875, 6958
XspI CTAG 8 cut(s) 2384, 2730, 5267, 5466, 6746, 6774, 7259, 7581
ZraI GACGTC 1 cut(s) 502
ZrmI AGTACT 5 cut(s) 587, 4658, 5072, 5754, 7705
Zsp2I ATGCAT 4 cut(s) 385, 2031, 2035, 5828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.