Rh3AG016000

Translational activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0004626
Physical Location & Seq
Forward (+)
1011058 .. 1032281
21224 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG016000.1

Sequence Viewer

Length: 7167 bp
ATGGCGGCTCCGCCAGAGGCTCTTCTCTCCGTCGCCGGGTCGGTCTCCACCGCCTCCACTCAGAAGCGGGTTCGGATCTTCCGAGATGAAATTCCGGCCATTATCGACGGCTCCGAAATATGTGTGGAGTCTGCAACACTGTTGGTAGATATAATATTTAAAACATTGTACATTTATGAGGATCGTGGGTCCAAGAAAGCTGTCGATGACATTATCACGAAATGTTTTCAAGAAGTTGCCTTCATGAAGAATTTTGCAGCAGCCCTTGTTCAGATGATGGAAAAAAATGTAAGGGTGCAATCACATGTTGGTGGCCACAGACTTTTCCAGTGGTCTTGTCTTCTTCTTAGCAAGAGTAAATTCGCCACAGTTTCCAAGAATGCATTCTGTAGAGTTGCCACAGTGCAGGCATCATTACTTCATATTGTTGTACAAACATCTCTCAGTGAGCAACAGGCTTGCAAGAAGACATTTTATCACTTACTTTCTCAGTCGCTGGACATCTACAAGTTGTATATAGAGGAGTTGAAGGAAGCACGCATCCCATACAAAGATAGTCCTGAATTATTATGGTTCTTGATGGAATTCTCAAGTACCTCAAGAAAATCATCTTCTTTGTTTGAACAATGCAAGCCAATATTTCTTGATATATATCTCAAGGCAATTTTAAACGCAAAAGAAAAGCCAGCAAAGGGTCTTAGCGAAGCTTTTCGTCCACTTTTTCGGCATATGTTGCATGAAGATTTTCAAAATGTTGTGCTTCCATCTTCTGTTAAAATGCTGAAACGCAACCCTGAGATTGTATTGGAATCAGTTGGCATTCTCTTGAAGTCAGTCAACATTGATTTGAGCAAGTATGCTGTCGAAATCCTTTCACTGGTCTTGCCACAGGCTCGGCATGCAGATGAAGGCCGGAGGGTTGGTGCACTGGAAATTGTACGATGTCTGAGCCAAAATTCTAGTAATCCAGATGCTATAGAAGCAATGGTCAATGCTGTCAAATCTGTCCTTGGAGGCTCAGAGGGAAGACTTACATTTCCTTACCAGAGAATTGGCATGATCAATGCACTGCAAGAATTGTGTAATGCTCCAGATGGGAAGCACCTTAATAGACTGTCACAGACCTCATGCAATTACCTTCGATCTTGTTACAAGGAGGATGGCAATGAGGAGGTCAAGCTTGCAATTTTGTCAGCTCTAGGTTCGTGGGCAGCAAGATCTGCTGATGTTGTTCAGTCAGATTTAGTTTCTTTTTTGTCTTCTGGGCTGAAGGAGAAAGAAGCTCTAAGAAGAGGGCATCTTCGTTGTCTACGAGCCATATGCAGAAATACAGATGCTGTTTATCGGTTATCATCATTACTAGAACCTCTCATTCAACTTGTCAAAACTGGTTTTACTAAAGGAGTTCAGCGTTTGGATGGTATATATGCCTTGCTATTAGTTGGCAAAATTGCAGCAGTTGACATCAAAGCAGAGGAGACCATGATGAAGGAAAAGATCTGGTCTGTGATATCTCAAAATGAGCCATCACTTCTTCCAATATCCTTGGCTTCAAAATTGTTGACTGAGGACTGCATGGCATGTGTCGATCTTCTTGAAGTCATGCTGGTGGAACATTTGCAGAGGGCGTTGGATTCCTTTTCTGTCAGATCACTGTCACAGTTGATAATATTTTTCATGTGCCACCCATGTTGGGATATTCGAAGAATGGCTTATAATGCTACCAAAAAGATAGTTGCTGCCGCCCCACAATTAGCTGAACATCTTTTGATTGAATTCACGACTTTCATGTCTGTAGTTGAGGAGAAACATCGTATTTCAAAGTCAAGTGATACAGATAGTTCATTGGACTCCCAAGTACCGTTCCTTCCCTCGGTAGAGGTTTCAGTAAAGGCTTTACTTGTTATATCATCAGCAGTTTTACCTGCAGCTCCAAGTGCATCAATGCGAGTTTTATTATGCGGACATCATCCATACTTAGTTGGAACTGCAAAAAGAGATGCAGTTTGGAGGAGGCTACACAAATGTTTGCATAAATGTGGTTTTGACATAAACAGCAATATTTTGGCTGACATGGAGAAATTGTGCAAGGGCCTACTGGAAACAATGTGGTTGTCCAGTGCTACTCAATCAGAGCAGCAAGCAGCAATATCCTCATTAGCCACTCTGATGTCCATTGCACCTGGAGAAACGTATGTGGAGTTTGAAAAGCACTTGAAACACCTTCCTTACCGATATTCACATGATACGCTTTCCGAAAATGATATTCGGATTTTTCATACTCCTGAAGGCTTGCTTTCCAGTGAGCAAGGCGTCTACATAGCTGAGTCTGTTGCTGCCAAGAACATGAAACAAGCTAAGGGCCGTTTTCGGATGTATGAGGACCTAAATGATATGGATAATGGTGGTTCTAATCATTCTGCAAAAGTCGATCCAAGCAGTAAAACTGGGAAGTCGACAAAGAAACCTGAGAAAACAAAGACTGCAAAAGAGGAGGCACGTGAGTTGCAACTTAAGGAGGAGGCATCCATACGTGAGAAGGTCCGAGAAATACAGAAAAACCTGTCTTTAATGCTTAAAGCTCTTGGGGAAATGGCCATTGCAAATCCAGTTTTTGCACATAGTCGACTTCCTTCCCTGGTTAACTATGTCAATCCATTACTAAGGTCACCAATAGTCAGCGATATTGCTTATGAAACTATGGTTCTTTATCATGTTCTTGGTATTGTACAAGCTTATCAATCCTCTATTGGACCTGCGTTGAATGAGTTGTGTCTTGGTCTGCAGCCAGATGAAGTTGCACCGGCTTTATACGGGGTTTATGCAAAAGATGTTCATGTTAGAATGGCTTGTCTAACTGCTGTCAAATGCATTCCTACTGTTGCTAGTCGGTCTCTTATTCAGAATGTTGAGGTTGCTACAAGTATTTGGATTGCTTTACATGACCCAGAAAAGGCCGATCCTAATGCTGATGTTCGGGGGAGAATGATTAATGCTGGCATTATGATTATTGATAAGCACGGAAGAGATAACGTGTCCTTGCTATTTCCCATCTTTGAGAACTATTTAAACAAAAAGGCATCGGATGAGGAGACATATGATTTGGTTCGTGAGGGTGTTGTTATTTTCACTGGAGCGTTGGCAAAGCATTTAGCAAAGGATGATCCAAAAGTACATGCTGTTATAGAGAAGTTGTTGGATGTGTTGAACACTCCATCAGAAGCTGTTCAGCGGGCAGTTTCAACATGCCTATCACCATTAATGCAATCAAAGCAAGATGATGGCCAAGCTCTTGTTTCAAGGATTTTGGATCAATTGATGAACAGTGATAAGTATGGGGAACGCCGTGGAGCTGCTTTTGGGCTTGCTGGAGTAGTCAAGGGATTTGGTATTTCTTCCTTGAAGAAGTATGGAATTGTGAATATACTGCAGGAAGGACTTGTTGATAGGAATTCTGCAAAACGTCGTGAAGGGGGTTTGCTTGGATTTGAGTGTTTATGTGAAACACTTGGAAAATTATTTGAACCGTATGTAATACAAATGTTGCCACTACTGTTGGTTTCATTTTCTGATCAAGTTGTTGCAGTTCGTGAAGGTGCTGAGTGTGCTGCTCGGGCAATGATGTCTCACCTTACTGCTCAGGGAGTGAAACTGGTCCTTCCATCTCTGTTGAAGGGTCTTGAAGATAAAGCTTGGCGAACAAAGCAAAGTAGTGTTCAACTGCTTGGTGCTATGGCCTATTGTGCTCCTAAGCAGCTGTCTCAATGTCTCCCTAAGATTGTACCGAAACTGACTGAGGTTTTAACAGACACCCACCCTAAAGTTCAGTCAGCAGGACAAACAGCACTTCAACAGGTTGGGAGTGTAATAAAGAATCCTGAGATAGCTTCATTGGTCCCTACTCTTCTTCTGGGTCTTACAGATCCAAATGACTATACAAAATATTCTCTGGATGTTCTCTTGGGAACAACATTCATCAATTCAATTGATGCTCCTTCACTTGCACTCTTGGTGCCGATAGTACATAGAGGGCTAAGGGAGAGGGGTGCAGAGACTAAAAAGAAAGCAGCCCAAATAGTTGGCAATATGTGTTCGTTAGTTACAGATCCAAATGATATGATTCCGTACATAGGGCTGCTTCTTCCTGAAGTGAAAAAGGTTCTTGTGGATCCAATTCCAGAAGTTAGGTCTGTTGCTGCAAGGGCGCTTGGATCCCTCATAAGGGGAATGGGTGAAGATCATTTCCCAGATCTTGTTCCCTGGTTGTTGGATACACTTAAATCAGATAATAGTAATGTTGAGCGATCTGGAGCTGCTCAAGGGTTAAGTGAGGTATTAGCTGCACTTGGGACTGAGTACTTTGAGCATGTACTTCCTGATGTTATCCGAAATTGTTCTCATCAAAAAGCATCTGTGCGTGATGGGCATTTGACATTATTCAAGTATTTACCAAGGTCCTTAGGCGTCCAATTCCAGAATTATTTACAGCAGGTGCTGCCTGCTATTATAGATGGTCTTGCTGATGAAAATGAGTCTGTACGTGAAGCAGCATTAGGTGCGGGACATGTTCTGGTTGAGCATTATGCTATAACGTCGTTACCTTTGCTCCTACCCGCTGTTGAGGATGGTATCTTTAATGATAGTTGGCGAATTCGACAAAGCTCTGTGGAATTATTGGGGGATCTCTTATTTAAGGTTGCGGGTACTTCTGGAAAAGCTTTACTGGAGGGTGGCAGTGATGATGAAGGTGCAAGTACTGAAGCACAGGGGCGGGCTATCATTGAGGTTCTTGGAAGGGATAAGCGTGATGAAATTCTCGCTGCATTATATATGGTTCGAACTGATGTCAGCCTATCAGTGCGTCAGGCTGCGTTGCATGTATGGAAAACTATAGTGGCAAATACCCCAAAAACTCTAAAGGAAATAATGCCAGTTCTAATGAATACTCTAATTACCTCTCTTGCATCATCATCCTTTGAAAGGCGGCAGGTCGCTGCACGTGCACTGGGTGAGCTTGTCAGGAAGCTTGGTGAAAGAGTGCTGCCATTGATCATCCCAATTTTATCCCAAGGATTGAAGGACTCTGACACCAGTAGACGACAAGGTGTATGCATTGGTCTGAGTGAAGTAATGGCATCTGCAGCTAAAAGTCATTTGTTGAGTTTCATGGATGAGCTCATTCCAACCATTCGAACTGCTCTTTCAGATAGCATGCCCGAAGTTCGTGAGTCTGCTGGAATAGCATTCAGCACTCTGTACAAGAATGCTGGAATGCAAGCGATTGATGAAATTGTTCCCAGTCTTTTGCATGCTTTGGAGGACGATCAAACTTCTGATACTGCACTTGATGGTCTCAAACAAATCTTAAGTGTCAGGATTACTGCAGTTTTACCCCACATTTTGCCCAAGCTTGTCCAGCTTCCACTAACTGCATTTAATGCACATGCTCTTGGAGCTGTAGCTGAGGTTGCTGGGCCTGGTCTTAACACTCATCTTGGTACCGTTCTTCCTGCTTTACTTACTTCAATGGGTGTTGATGCAAAGGATGTTCAAACATTGGCAAAGGAGGCTGCTGAAACTGTGGTCTTAGTAATAGATGAGGAAGGTGTTGAATATCTTACATCTGAGCTACTTAGAGCTGTCAGCGATAGTCAGGCTTCAATTAGAAGAAGTGCCGCGTATCTTATAGGCTATTTCTTCAAAAACAGTAAATTATATTTGGTTGATGAAGCGCCAAACATGATATCTACCTTGATTGTCTTGCTCAGTGATTCTGATTCGGCTACTGTGGCGGTATCTTGGGAAGCATTGTCAAGGGTTGTAAGTTCAGTCCCCAAGGAGGTACTTCCTTCATATATTAAATTAGTACGAGATGCTGTGTCGACTTCCAGAGATAAGGAGCGCAGGAAAAAGAAGGGAGGACCTATTGTCATACCTGGATTTTGTTTACCAAAAGCACTTCAACCGTTGCTTCCAATATTTCTCCAGGGTTTAATAAGTGGATCTGCTGAACTGAGAGAGCAAGCGGCACTGGGTCTTGGAGAACTGATTGAAGTGACTAGTGAAAAGGCACTCAAAGAGTTTGTCATTCCAATTACAGGGCCTCTTATTCGGATAATAGGTGACCGGTTCCCTTGGCAGGTGAAGAGTGCTATTTTATCTACCTTGACAATCATAATCAGAAAAGGTGGGATGGCCTTGAAACCATTTCTTCCGCAGCTTCAAACAACATTTGTCAAGTGTCTACAGGATAACACAAGAATTGTTCGCTCAAGTGCTGCCCTTGCTCTCGGAAAGCTTAGTGCTTTAAGTACCCGGGTGGACCCTCTAGTTGGAGACCTCCTGTCCAGTCTGCAGGCTATGGATAGTGGGGTTCGGGAGGCAAGTTTGTCCGCTTTAGAAGGTGTCCTAAAGCATGCTGGAAAGAGCGTGAGCAGTGCTGTTAGAACTCGTGTCTACTTGCAGCTTAAGGATTTGATTCATCATGATGATGATGAGGTTCGAGTATCTGCTGCAAGCATTTTGGGTATCATGTCCCAGTACATTGAAGATACCCAGCTGATCGAGCTGCTTCAGGAACTATCAAGTTTTCCATCGTCGCTCAGCTGGACTGCTAGGCATGGTTCTGTACTTACAATTTCATCCATGCTCAGGCACATCCCATCCACCATCTGTACTTCTCCAGTGTTTCCGTCTATCCTGGACCAGCTTAAAGCTGCCTTGACTGATGAGAAGTTCCCTCTTCGTGAAACTTCAACCAAGGCATTTGGGAGACTTCTTGTTTATAAAGTCCGAAGTGATCCTTCTAATACCTCAGTACAATTAGATATTATATCGTCTTTAGTCTCAGCATTGCATGATGATTCTAGCGAAGTTAGAAGGAAGGCGTTATCTGCTCTAAAGGCAGTTTCGAAGGAAAGTTGTTCATCTATTGCGGCCCATATCAATATTATTGGACCTGCACTGGCTGAATGTTTGAAGGATGGAAGTACACCCGTGAGACTTGCAGCAGAAAGATGTGCTCTGCATGCCTTCCAATTGACAAAGGGTCCTGAAAATGTTCAAGCTGCACAAAAGTTCATCACAGGCTTGGAAGCTAGACGAATATCAAAGTTTTCAGAACATAGTGATGATAGTGAAGGTGAGGATGACTTGGCAAGTGGCTGA

Protein Analysis

2388

Amino Acids

261.17

Weight (kDa)

7.01

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GNC1_N PF24993 211 - 344 1.4e-45 Stalled ribosome sensor GCN1 N-terminal
HEAT_EF3_N PF24987 1115 - 1282 1.2e-46 EF3 N-terminal HEAT repeat
HEAT_GCN1 PF23271 1165 - 1302 2.4e-10 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_GNC1 PF24984 1320 - 1406 5.2e-26 Elongation factor 3/GNC1 HEAT repeat
TOR1L1_N PF24714 1325 - 1518 8.1e-06 TORTIFOLIA1 N-terminal
HEAT_MROH2B_C PF23227 1383 - 1517 9.3e-06 MROH2B-like, HEAT repeats
HEAT_GCN1 PF23271 1394 - 1541 1.8e-11 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_N PF24987 1435 - 1564 2.4e-08 EF3 N-terminal HEAT repeat
TPR_DNAAF5 PF25757 1603 - 1759 6.9e-07 Dynein axonemal assembly factor 5-like, TPR repeats
HEAT_GCN1 PF23271 1637 - 1783 4.3e-07 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_N PF24987 1650 - 1745 1.3e-07 EF3 N-terminal HEAT repeat
HEAT_EF3_GNC1 PF24984 1673 - 1752 5.6e-06 Elongation factor 3/GNC1 HEAT repeat
HEAT_GCN1 PF23271 1796 - 1985 3.5e-52 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_MROH2B_C PF23227 2079 - 2188 4.6e-07 MROH2B-like, HEAT repeats
HEAT_GCN1_C_2 PF25801 2108 - 2359 3.9e-31 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_GCN1_C PF25786 2206 - 2360 2e-74 GCN1-like, C-terminal HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1716, 6786
AarI CACCTGC 2 cut(s) 4480, 6130
AasI GACNNNNNNGTC 1 cut(s) 1789
Acc36I ACCTGC 6 cut(s) 1933, 2764, 4480, 4978, 6130, 6967
Acc65I GGTACC 1 cut(s) 5498
AccB1I GGYRCC 2 cut(s) 4012, 5498
AccI GTMKAC 8 cut(s) 1309, 2316, 2456, 2626, 5095, 5882, 6244, 6456
AccII CGCG 1 cut(s) 5678
AcoI YGGCCR 4 cut(s) 96, 313, 2595, 3283
AcsI RAATTY 9 cut(s) 90, 250, 359, 584, 955, 1775, 3451, 4650, 4812
AcuI CTGAAG 5 cut(s) 1289, 2307, 4167, 4779, 6557
AcvI CACGTG 2 cut(s) 2501, 5000
AcyI GRCGYC 2 cut(s) 2313, 4464
AdeI CACNNNGTG 1 cut(s) 5009
AflII CTTAAG 3 cut(s) 2513, 5365, 6467
AflIII ACRYGT 3 cut(s) 304, 3033, 4564
AgeI ACCGGT 1 cut(s) 6126
AhdI GACNNNNNGTC 3 cut(s) 5927, 6343, 7047
AhlI ACTAGT 1 cut(s) 6059
AjnI CCWGG 7 cut(s) 2182, 2637, 4259, 5476, 5935, 5985, 6699
AjuI GAANNNNNNNTTGG 2 cut(s) 368, 400
Alw21I GWGCWC 5 cut(s) 928, 3748, 5005, 5178, 7024
Alw44I GTGCAC 2 cut(s) 924, 5001
AlwNI CAGNNNCTG 4 cut(s) 496, 1337, 3224, 4495
Ama87I CYCGRG 2 cut(s) 3612, 6315
ApaLI GTGCAC 2 cut(s) 924, 5001
ApoI RAATTY 9 cut(s) 90, 250, 359, 584, 955, 1775, 3451, 4650, 4812
ArsI GACNNNNNNTTYG 4 cut(s) 3085, 3117, 6069, 6101
AseI ATTAAT 2 cut(s) 2991, 3260
AsiGI ACCGGT 1 cut(s) 6126
Asp700I GAANNNNTTC 7 cut(s) 383, 708, 744, 3225, 4393, 5292, 7059
Asp718I GGTACC 1 cut(s) 5498
AspLEI GCGC 3 cut(s) 4207, 5734, 5904
AsuC2I CCSGG 3 cut(s) 37, 6316, 6317
AsuHPI GGTGA 9 cut(s) 2661, 3246, 3620, 4244, 5021, 5042, 6134, 6154, 7154
AsuII TTCGAA 4 cut(s) 1702, 4837, 5191, 6911
AvaI CYCGRG 2 cut(s) 3612, 6315
AxyI CCTNAGG 1 cut(s) 4459
BaeGI GKGCMC 2 cut(s) 928, 5005
BalI TGGCCA 3 cut(s) 315, 2597, 3285
BamHI GGATCC 2 cut(s) 4168, 4211
BanI GGYRCC 2 cut(s) 4012, 5498
BanII GRGCYC 1 cut(s) 5178
BarI GAAGNNNNNNTAC 2 cut(s) 1851, 1883
BauI CACGAG 1 cut(s) 6450
BbrPI CACGTG 2 cut(s) 2501, 5000
BbsI GAAGAC 4 cut(s) 332, 473, 1033, 1251
Bbv12I GWGCWC 5 cut(s) 928, 3748, 5005, 5178, 7024
BbvCI CCTCAGC 1 cut(s) 5463
BceAI ACGGC 3 cut(s) 124, 2349, 3330
BcgI CGANNNNNNTGC 4 cut(s) 1302, 1336, 2948, 2982
BciT130I CCWGG 7 cut(s) 2184, 2639, 4261, 5478, 5937, 5987, 6701
BciVI GTATCC 1 cut(s) 4264
BclI TGATCA 3 cut(s) 1059, 3571, 5049
BcnI CCSGG 3 cut(s) 37, 6316, 6317
BcuI ACTAGT 1 cut(s) 6059
BfaI CTAG 9 cut(s) 960, 1199, 1361, 2886, 6060, 6329, 6615, 6867, 7098
BfoI RGCGCY 2 cut(s) 4208, 5735
BfrI CTTAAG 3 cut(s) 2513, 5365, 6467
BfuAI ACCTGC 6 cut(s) 1933, 2764, 4480, 4978, 6130, 6967
BfuI GTATCC 1 cut(s) 4264
BglII AGATCT 3 cut(s) 1217, 1497, 4249
BlpI GCTNAGC 1 cut(s) 6602
BmcAI AGTACT 2 cut(s) 4358, 4756
BmeRI GACNNNNNGTC 3 cut(s) 5927, 6343, 7047
BmeT110I CYCGRG 2 cut(s) 3612, 6315
BmrI ACTGGG 5 cut(s) 2457, 5015, 5292, 6041, 6532
BmuI ACTGGG 5 cut(s) 2457, 5015, 5292, 6041, 6532
BpiI GAAGAC 4 cut(s) 332, 473, 1033, 1251
BpmI CTGGAG 8 cut(s) 1074, 2205, 3153, 3390, 4329, 4745, 5969, 6666
Bpu10I CCTNAGC 6 cut(s) 2358, 3639, 3750, 4034, 5463, 6650
Bpu1102I GCTNAGC 1 cut(s) 6602
Bpu14I TTCGAA 4 cut(s) 1702, 4837, 5191, 6911
BpuEI CTTGAG 5 cut(s) 574, 583, 641, 4302, 6256
BpuMI CCSGG 3 cut(s) 37, 6316, 6317
BsaAI YACGTR 4 cut(s) 2501, 2534, 4541, 5000
BsaBI GATNNNNATC 1 cut(s) 651
BsaHI GRCGYC 2 cut(s) 2313, 4464
BsaI GGTCTC 5 cut(s) 49, 1472, 2898, 5357, 6330
BsaWI WCCGGW 1 cut(s) 6126
BsaXI ACNNNNNCTCC 6 cut(s) 907, 937, 2069, 2099, 4590, 4620
Bse118I RCCGGY 2 cut(s) 2803, 6126
Bse21I CCTNAGG 1 cut(s) 4459
Bse3DI GCAATG 6 cut(s) 990, 1171, 2175, 2598, 3624, 6851
Bse8I GATNNNNATC 1 cut(s) 651
BseBI CCWGG 7 cut(s) 2184, 2639, 4261, 5478, 5937, 5987, 6701
BseJI GATNNNNATC 1 cut(s) 651
BseMI GCAATG 6 cut(s) 990, 1171, 2175, 2598, 3624, 6851
BseRI GAGGAG 8 cut(s) 536, 1184, 1490, 1817, 2026, 2507, 2534, 3104
BseSI GKGCMC 2 cut(s) 928, 5005
BseYI CCCAGC 2 cut(s) 5471, 6555
BsgI GTGCAG 7 cut(s) 425, 4068, 4326, 4980, 5325, 6945, 7053
Bsh1236I CGCG 1 cut(s) 5678
BshNI GGYRCC 2 cut(s) 4012, 5498
BshTI ACCGGT 1 cut(s) 6126
BsiHKAI GWGCWC 5 cut(s) 928, 3748, 5005, 5178, 7024
BsiHKCI CYCGRG 2 cut(s) 3612, 6315
BsiSI CCGG 6 cut(s) 36, 95, 913, 2804, 6127, 6316
BslFI GGGAC 5 cut(s) 3881, 4363, 4575, 5816, 6520
BsmFI GGGAC 5 cut(s) 3881, 4363, 4575, 5816, 6520
BsmI GAATGC 7 cut(s) 383, 385, 819, 2871, 5243, 5269, 5277
Bso31I GGTCTC 5 cut(s) 49, 1472, 2898, 5357, 6330
BsoBI CYCGRG 2 cut(s) 3612, 6315
Bsp119I TTCGAA 4 cut(s) 1702, 4837, 5191, 6911
Bsp1286I GDGCHC 5 cut(s) 928, 3748, 5005, 5178, 7024
Bsp1407I TGTACA 4 cut(s) 168, 430, 2728, 5256
Bsp1720I GCTNAGC 1 cut(s) 6602
BspFNI CGCG 1 cut(s) 5678
BspHI TCATGA 2 cut(s) 243, 6484
BspMAI CTGCAG 6 cut(s) 1930, 2787, 3432, 5143, 5386, 6357
BspMI ACCTGC 6 cut(s) 1933, 2764, 4480, 4978, 6130, 6967
BspQI GCTCTTC 1 cut(s) 27
BspT104I TTCGAA 4 cut(s) 1702, 4837, 5191, 6911
BspT107I GGYRCC 2 cut(s) 4012, 5498
BspTI CTTAAG 3 cut(s) 2513, 5365, 6467
BspTNI GGTCTC 5 cut(s) 49, 1472, 2898, 5357, 6330
BsrDI GCAATG 6 cut(s) 990, 1171, 2175, 2598, 3624, 6851
BsrFI RCCGGY 2 cut(s) 2803, 6126
BsrGI TGTACA 4 cut(s) 168, 430, 2728, 5256
BssAI RCCGGY 2 cut(s) 2803, 6126
BssNI GRCGYC 2 cut(s) 2313, 4464
BssSI CACGAG 1 cut(s) 6450
BssT1I CCWWGG 7 cut(s) 1009, 1545, 4451, 5068, 5835, 6134, 6759
Bst2BI CACGAG 1 cut(s) 6450
Bst2UI CCWGG 7 cut(s) 2184, 2639, 4261, 5478, 5937, 5987, 6701
Bst6I CTCTTC 6 cut(s) 27, 1285, 3019, 3909, 6140, 6747
BstACI GRCGYC 2 cut(s) 2313, 4464
BstAFI CTTAAG 3 cut(s) 2513, 5365, 6467
BstAPI GCANNNNNTGC 5 cut(s) 733, 1220, 4495, 4556, 5438
BstAUI TGTACA 4 cut(s) 168, 430, 2728, 5256
BstBAI YACGTR 4 cut(s) 2501, 2534, 4541, 5000
BstBI TTCGAA 4 cut(s) 1702, 4837, 5191, 6911
BstDSI CCRYGG 1 cut(s) 3346
BstEII GGTNACC 2 cut(s) 2667, 6122
BstENI CCTNNNNNAGG 1 cut(s) 4978
BstFNI CGCG 1 cut(s) 5678
BstH2I RGCGCY 2 cut(s) 4208, 5735
BstHHI GCGC 3 cut(s) 4207, 5734, 5904
BstNI CCWGG 7 cut(s) 2184, 2639, 4261, 5478, 5937, 5987, 6701
BstPI GGTNACC 2 cut(s) 2667, 6122
BstSLI GKGCMC 2 cut(s) 928, 5005
BstUI CGCG 1 cut(s) 5678
BstV2I GAAGAC 4 cut(s) 332, 473, 1033, 1251
BstXI CCANNNNNNTGG 2 cut(s) 1052, 4079
Bsu36I CCTNAGG 1 cut(s) 4459
BsuI GTATCC 1 cut(s) 4264
BtgI CCRYGG 1 cut(s) 3346
BtsI GCAGTG 3 cut(s) 1067, 4741, 6442
BveI ACCTGC 6 cut(s) 1933, 2764, 4480, 4978, 6130, 6967
CaiI CAGNNNCTG 4 cut(s) 496, 1337, 3224, 4495
CciI TCATGA 2 cut(s) 243, 6484
CfoI GCGC 3 cut(s) 4207, 5734, 5904
Cfr10I RCCGGY 2 cut(s) 2803, 6126
Cfr9I CCCGGG 1 cut(s) 6315
CseI GACGC 3 cut(s) 2302, 4453, 4850
CspAI ACCGGT 1 cut(s) 6126
CspCI CAANNNNNGTGG 4 cut(s) 1673, 1708, 3537, 3572
DraI TTTAAA 3 cut(s) 160, 669, 3069
DraIII CACNNNGTG 1 cut(s) 5009
DrdI GACNNNNNNGTC 1 cut(s) 1789
DriI GACNNNNNGTC 3 cut(s) 5927, 6343, 7047
DseDI GACNNNNNNGTC 1 cut(s) 1789
EaeI YGGCCR 4 cut(s) 96, 313, 2595, 3283
Eam1104I CTCTTC 6 cut(s) 27, 1285, 3019, 3909, 6140, 6747
Eam1105I GACNNNNNGTC 3 cut(s) 5927, 6343, 7047
EarI CTCTTC 6 cut(s) 27, 1285, 3019, 3909, 6140, 6747
Ecl136II GAGCTC 1 cut(s) 5176
Eco130I CCWWGG 7 cut(s) 1009, 1545, 4451, 5068, 5835, 6134, 6759
Eco24I GRGCYC 1 cut(s) 5178
Eco31I GGTCTC 5 cut(s) 49, 1472, 2898, 5357, 6330
Eco32I GATATC 2 cut(s) 1512, 5745
Eco53kI GAGCTC 1 cut(s) 5176
Eco57I CTGAAG 5 cut(s) 1289, 2307, 4167, 4779, 6557
Eco72I CACGTG 2 cut(s) 2501, 5000
Eco81I CCTNAGG 1 cut(s) 4459
Eco88I CYCGRG 2 cut(s) 3612, 6315
Eco91I GGTNACC 2 cut(s) 2667, 6122
EcoICRI GAGCTC 1 cut(s) 5176
EcoNI CCTNNNNNAGG 1 cut(s) 4978
EcoO109I RGGNCCY 6 cut(s) 2092, 2383, 4455, 5921, 6101, 7049
EcoO65I GGTNACC 2 cut(s) 2667, 6122
EcoRI GAATTC 4 cut(s) 584, 1775, 3451, 4650
EcoRII CCWGG 7 cut(s) 2182, 2637, 4259, 5476, 5935, 5985, 6699
EcoRV GATATC 2 cut(s) 1512, 5745
EcoT14I CCWWGG 7 cut(s) 1009, 1545, 4451, 5068, 5835, 6134, 6759
EcoT22I ATGCAT 3 cut(s) 385, 2873, 5114
EcoT38I GRGCYC 1 cut(s) 5178
ErhI CCWWGG 7 cut(s) 1009, 1545, 4451, 5068, 5835, 6134, 6759
FalI AAGNNNNNCTT 6 cut(s) 1696, 1728, 2280, 2312, 6787, 6819
FaqI GGGAC 5 cut(s) 3881, 4363, 4575, 5816, 6520
FauI CCCGC 6 cut(s) 60, 3225, 4552, 4621, 4693, 4764
FauNDI CATATG 3 cut(s) 729, 1319, 3097
FbaI TGATCA 3 cut(s) 1059, 3571, 5049
FblI GTMKAC 8 cut(s) 1309, 2316, 2456, 2626, 5095, 5882, 6244, 6456
FriOI GRGCYC 1 cut(s) 5178
FspBI CTAG 9 cut(s) 960, 1199, 1361, 2886, 6060, 6329, 6615, 6867, 7098
GlaI GCGC 3 cut(s) 4206, 5733, 5903
GsaI CCCAGC 2 cut(s) 5475, 6559
GsuI CTGGAG 8 cut(s) 1074, 2205, 3153, 3390, 4329, 4745, 5969, 6666
HaeII RGCGCY 2 cut(s) 4208, 5735
HapII CCGG 6 cut(s) 36, 95, 913, 2804, 6127, 6316
HgaI GACGC 3 cut(s) 2302, 4453, 4850
HhaI GCGC 3 cut(s) 4207, 5734, 5904
Hin1I GRCGYC 2 cut(s) 2313, 4464
Hin6I GCGC 3 cut(s) 4205, 5732, 5902
HinP1I GCGC 3 cut(s) 4205, 5732, 5902
HincII GTYRAC 7 cut(s) 838, 1462, 1563, 2457, 2627, 2644, 5883
HindII GTYRAC 7 cut(s) 838, 1462, 1563, 2457, 2627, 2644, 5883
HindIII AAGCTT 8 cut(s) 705, 1178, 2733, 3690, 4716, 5024, 5408, 6296
HpaI GTTAAC 1 cut(s) 2644
HpaII CCGG 6 cut(s) 36, 95, 913, 2804, 6127, 6316
HphI GGTGA 9 cut(s) 2661, 3246, 3620, 4244, 5021, 5042, 6134, 6154, 7154
Hpy99I CGWCG 5 cut(s) 35, 110, 3468, 4597, 6601
HpyCH4IV ACGT 8 cut(s) 2192, 2500, 2533, 3033, 3463, 4540, 4592, 4999
HpySE526I ACGT 8 cut(s) 2192, 2500, 2533, 3033, 3463, 4540, 4592, 4999
Hsp92I GRCGYC 2 cut(s) 2313, 4464
HspAI GCGC 3 cut(s) 4205, 5732, 5902
KpnI GGTACC 1 cut(s) 5502
Ksp22I TGATCA 3 cut(s) 1059, 3571, 5049
KspAI GTTAAC 1 cut(s) 2644
LguI GCTCTTC 1 cut(s) 27
MaeI CTAG 9 cut(s) 960, 1199, 1361, 2886, 6060, 6329, 6615, 6867, 7098
MaeII ACGT 8 cut(s) 2192, 2500, 2533, 3033, 3463, 4540, 4592, 4999
MaeIII GTNAC 8 cut(s) 1116, 1148, 1656, 2667, 4099, 4596, 6055, 6122
MfeI CAATTG 3 cut(s) 3314, 3984, 7037
MhlI GDGCHC 5 cut(s) 928, 3748, 5005, 5178, 7024
MlsI TGGCCA 3 cut(s) 315, 2597, 3285
MluNI TGGCCA 3 cut(s) 315, 2597, 3285
MlyI GAGTC 6 cut(s) 137, 1844, 2336, 4541, 5075, 5237
MmeI TCCRAC 6 cut(s) 1611, 1963, 3177, 4248, 5207, 6313
Mox20I TGGCCA 3 cut(s) 315, 2597, 3285
Mph1103I ATGCAT 3 cut(s) 385, 2873, 5114
MroXI GAANNNNTTC 7 cut(s) 383, 708, 744, 3225, 4393, 5292, 7059
MscI TGGCCA 3 cut(s) 315, 2597, 3285
MslI CAYNNNNRTG 8 cut(s) 309, 903, 1607, 2037, 2168, 6486, 6489, 7128
Msp20I TGGCCA 3 cut(s) 315, 2597, 3285
MspA1I CMGCKG 5 cut(s) 3232, 3757, 4616, 6559, 6606
MspCI CTTAAG 3 cut(s) 2513, 5365, 6467
MspI CCGG 6 cut(s) 36, 95, 913, 2804, 6127, 6316
MunI CAATTG 3 cut(s) 3314, 3984, 7037
Mva1269I GAATGC 7 cut(s) 383, 385, 819, 2871, 5243, 5269, 5277
MvaI CCWGG 7 cut(s) 2184, 2639, 4261, 5478, 5937, 5987, 6701
MvnI CGCG 1 cut(s) 5678
NciI CCSGG 3 cut(s) 37, 6316, 6317
NdeI CATATG 3 cut(s) 729, 1319, 3097
NmeAIII GCCGAG 1 cut(s) 874
NmuCI GTSAC 5 cut(s) 1116, 1656, 2667, 6055, 6122
NsiI ATGCAT 3 cut(s) 385, 2873, 5114
NspV TTCGAA 4 cut(s) 1702, 4837, 5191, 6911
PaeI GCATGC 5 cut(s) 902, 5215, 5312, 6419, 7031
PagI TCATGA 2 cut(s) 243, 6484
PaqCI CACCTGC 2 cut(s) 4480, 6130
PciI ACATGT 2 cut(s) 304, 4564
PciSI GCTCTTC 1 cut(s) 27
PcsI WCGNNNNNNNCGW 3 cut(s) 79, 111, 1309
PctI GAATGC 7 cut(s) 383, 385, 819, 2871, 5243, 5269, 5277
PdmI GAANNNNTTC 7 cut(s) 383, 708, 744, 3225, 4393, 5292, 7059
PfeI GAWTC 8 cut(s) 809, 1634, 3874, 4120, 5771, 5777, 6478, 6863
PfoI TCCNGGA 1 cut(s) 6699
PinAI ACCGGT 1 cut(s) 6126
PleI GAGTC 6 cut(s) 136, 1844, 2335, 4540, 5075, 5236
PmaCI CACGTG 2 cut(s) 2501, 5000
PmlI CACGTG 2 cut(s) 2501, 5000
PpsI GAGTC 6 cut(s) 136, 1844, 2335, 4540, 5075, 5236
Ppu21I YACGTR 4 cut(s) 2501, 2534, 4541, 5000
PpuMI RGGWCCY 4 cut(s) 2383, 4455, 5921, 7049
PscI ACATGT 2 cut(s) 304, 4564
PshBI ATTAAT 2 cut(s) 2991, 3260
PsiI TTATAA 2 cut(s) 1716, 6786
Psp124BI GAGCTC 1 cut(s) 5178
Psp5II RGGWCCY 4 cut(s) 2383, 4455, 5921, 7049
Psp6I CCWGG 7 cut(s) 2182, 2637, 4259, 5476, 5935, 5985, 6699
PspCI CACGTG 2 cut(s) 2501, 5000
PspEI GGTNACC 2 cut(s) 2667, 6122
PspFI CCCAGC 2 cut(s) 5471, 6555
PspGI CCWGG 7 cut(s) 2182, 2637, 4259, 5476, 5935, 5985, 6699
PspPPI RGGWCCY 4 cut(s) 2383, 4455, 5921, 7049
PstI CTGCAG 6 cut(s) 1930, 2787, 3432, 5143, 5386, 6357
PstNI CAGNNNCTG 4 cut(s) 496, 1337, 3224, 4495
PvuII CAGCTG 3 cut(s) 3757, 6559, 6606
RseI CAYNNNNRTG 8 cut(s) 309, 903, 1607, 2037, 2168, 6486, 6489, 7128
SacI GAGCTC 1 cut(s) 5178
SalI GTCGAC 3 cut(s) 2455, 2625, 5881
SapI GCTCTTC 1 cut(s) 27
ScaI AGTACT 2 cut(s) 4358, 4756
SchI GAGTC 6 cut(s) 137, 1844, 2336, 4541, 5075, 5237
SduI GDGCHC 5 cut(s) 928, 3748, 5005, 5178, 7024
SfuI TTCGAA 4 cut(s) 1702, 4837, 5191, 6911
SmaI CCCGGG 1 cut(s) 6317
SmiMI CAYNNNNRTG 8 cut(s) 309, 903, 1607, 2037, 2168, 6486, 6489, 7128
SmlI CTYRAG 8 cut(s) 589, 598, 656, 2513, 4317, 5365, 6271, 6467
SmoI CTYRAG 8 cut(s) 589, 598, 656, 2513, 4317, 5365, 6271, 6467
SpeI ACTAGT 1 cut(s) 6059
SphI GCATGC 5 cut(s) 902, 5215, 5312, 6419, 7031
SspI AATATT 7 cut(s) 156, 639, 1671, 2062, 3944, 5979, 6949
SspMI CTAG 9 cut(s) 960, 1199, 1361, 2886, 6060, 6329, 6615, 6867, 7098
SstI GAGCTC 1 cut(s) 5178
StyI CCWWGG 7 cut(s) 1009, 1545, 4451, 5068, 5835, 6134, 6759
TaiI ACGT 8 cut(s) 2195, 2503, 2536, 3036, 3466, 4543, 4595, 5002
TaqII GACCGA 2 cut(s) 31, 2880
TauI GCSGC 6 cut(s) 8, 1745, 4987, 5678, 6029, 6938
TfiI GAWTC 8 cut(s) 809, 1634, 3874, 4120, 5771, 5777, 6478, 6863
TseFI GTSAC 5 cut(s) 1116, 1656, 2667, 6055, 6122
Tsp45I GTSAC 5 cut(s) 1116, 1656, 2667, 6055, 6122
TspGWI ACGGA 4 cut(s) 19, 3036, 4113, 6681
TspMI CCCGGG 1 cut(s) 6315
Vha464I CTTAAG 3 cut(s) 2513, 5365, 6467
VneI GTGCAC 2 cut(s) 924, 5001
VspI ATTAAT 2 cut(s) 2991, 3260
XagI CCTNNNNNAGG 1 cut(s) 4978
XapI RAATTY 9 cut(s) 90, 250, 359, 584, 955, 1775, 3451, 4650, 4812
XmaI CCCGGG 1 cut(s) 6315
XmiI GTMKAC 8 cut(s) 1309, 2316, 2456, 2626, 5095, 5882, 6244, 6456
XmnI GAANNNNTTC 7 cut(s) 383, 708, 744, 3225, 4393, 5292, 7059
XspI CTAG 9 cut(s) 960, 1199, 1361, 2886, 6060, 6329, 6615, 6867, 7098
ZrmI AGTACT 2 cut(s) 4358, 4756
Zsp2I ATGCAT 3 cut(s) 385, 2873, 5114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.