Rh3CG015200

Translational activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0004626
Physical Location & Seq
Forward (+)
1010985 .. 1032585
21601 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG015200.1

Sequence Viewer

Length: 7866 bp
ATGGCGGCTCCGCCAGAGGCTCTTCTCTCCGTCGCCGGGTCGGTCTCCACCGCCTCCACTCAGAAGCGGGTTCGGATCTTCCGAGATGAAATTCCGGCCATTATCGACGGCTCCGAAATATGTGTGGAGTCTGCAACACTGTTGGTAGATATAATATTTAAAACATTGTACATTTATGAGGATCGTGGGTCCAAGAAAGCTGTCGATGACATTATCACGAAATGTTTTCAAGAAGTTGCCTTCATGAAGAATTTTGCAGCAGCCCTTGTTCAGATGATGGAAAAAAATGTAAGGGTGCAATCACATGTTGGTGGCCACAGACTTTTCCAGTGGTCTTGTCTTCTTCTTAGCAAGAGTAAATTCGCCACAGTTTCCAAGAATGCATTCTGTAGAGTTGCCACAGTGCAGGCATCATTACTTCATATTGTTGTACAAACATCTCTCAGTGAGCAACAGGCTTGCAAGAAGACATTTTATCACTTACTTTCTCAGTCGCTGGACATCTACAAGTTGTATATAGAGGAGTTGAAGGAAGCACGCATCCCATACAAAGATAGTCCTGAATTATTATGGTTCTTGATGGAATTCTCAAGTACCTCAAGAAAATCATCTTCTTTGTTTGAACAATGCAAGCCAATATTTCTTGATATATATCTCAAGGCAATTTTAAACGCAAAAGAAAAGCCAGCAAAGGGTCTTAGCGAAGCTTTTCGTCCACTTTTTCGGCATATGTTGCATGAAGATTTTCAAAATGTTGTGCTTCCATCTTCTGTTAAAATGCTGAAACGCAACCCTGAGATTGTATTGGAATCAGTTGGCATTCTCTTGAAGTCAGTCAACATTGATTTGAGCAAGTATGCTGTCGAAATCCTTTCACTGGTCTTGCCACAGGCTCGGCATGCAGATGAAGGCCGGAGGGTTGGTGCACTGGAAATTGTACGATGTCTGAGCCAAAATTCTAGTAATCCAGATGCTATAGAAGCAATGGTCAATGCTGTCAAATCTGTCCTTGGAGGCTCAGAGGGAAGACTTACATTTCCTTACCAGAGAATTGGCATGATCAATGCACTGCAAGAATTGTGTAATGCTCCAGATGGGAAGCACCTTAATAGACTGTCACAGACCTCATGCAATTACCTTCGATCTTGTTACAAGGAGGATGGCAATGAGGAGGTCAAGCTTGCAATTTTGTCAGCTCTAGGTTCGTGGGCAGCAAGATCTGCTGATGTTGTTCAGTCAGATTTAGTTTCTTTTTTGTCTTCTGGGCTGAAGGAGAAAGAAGCTCTAAGAAGAGGGCATCTTCGTTGTCTACGAGCCATATGCAGAAATACAGATGCTGTTTATCGGTTATCATCATTACTAGAACCTCTCATTCAACTTGTCAAAACTGGTTTTACTAAAGGAGTTCAGCGTTTGGATGGTATATATGCCTTGCTATTAGTTGGCAAAATTGCAGCAGTTGACATCAAAGCAGAGGAGACCATGATGAAGGAAAAGATCTGGTCTGTGATATCTCAAAATGAGCCATCACTTCTTCCAATATCCTTGGCTTCAAAATTGTTGACTGAGGACTGCATGGCATGTGTCGATCTTCTTGAAGTCATGCTGGTGGAACATTTGCAGAGGGCGTTGGATTCCTTTTCTGTCAGATCACTGTCACAGTTGATAATATTTTTCATGTGCCACCCATGTTGGGATATTCGAAGAATGGCTTATAATGCTACCAAAAAGATAGTTGCTGCCGCCCCACAATTAGCTGAACATCTTTTGATTGAATTCACGACTTTCATGTCTGTAGTTGAGGAGAAACATCGTATTTCAAAGTCAAGTGATACAGATAGTTCATTGGACTCCCAAGTACCGTTCCTTCCCTCGGTAGAGGTTTCAGTAAAGGCTTTACTTGTTATATCATCAGCAGTTTTACCTGCAGCTCCAAGTGCATCAATGCGAGTTTTATTATGCGGACATCATCCATACTTAGTTGGAACTGCAAAAAGAGATGCAGTTTGGAGGAGGCTACACAAATGTTTGCATAAATGTGGTTTTGACATAAACAGCAATATTTTGGCTGACATGGAGAAATTGTGCAAGGGCCTACTGGAAACAATGTGGTTGTCCAGTGCTACTCAATCAGAGCAGCAAGCAGCAATATCCTCATTAGCCACTCTGATGTCCATTGCACCTGGAGAAACGTATGTGGAGTTTGAAAAGCACTTGAAACACCTTCCTTACCGATATTCACATGATACGCTTTCCGAAAATGATATTCGGATTTTTCATACTCCTGAAGGCTTGCTTTCCAGTGAGCAAGGCGTCTACATAGCTGAGTCTGTTGCTGCCAAGAACATGAAACAAGCTAAGGGCCGTTTTCGGATGTATGAGGACCTAAATGATATGGATAATGGTGGTTCTAATCATTCTGCAAAAGTCGATCCAAGCAGTAAAACTGGGAAGTCGACAAAGAAACCTGAGAAAACAAAGACTGCAAAAGAGGAGGCACGTGAGTTGCAACTTAAGGAGGAGGCATCCATACGTGAGAAGGTCCGAGAAATACAGAAAAACCTGTCTTTAATGCTTAAAGCTCTTGGGGAAATGGCCATTGCAAATCCAGTTTTTGCACATAGTCGACTTCCTTCCCTGGTTAACTATGTCAATCCATTACTAAGGTCACCAATAGTCAGCGATATTGCTTATGAAACTATGGTGAAACTCTCTAGATGTACTGCTCCCCCTCTTTGTAATTGGGGCCTTGACATTGCTACTGCTTTGCGCTTAGTTGTGACTGAAGAAGATCGTCTTCTGTTGGATATGATATCATCGGTTGGGCAAGGGGAAGCCGATGATAGACCATCTTTGAGTCTATTTGAGCGAATTATTAATGCCATTTCTGCATCTTGTAAATCGGGACCTCTTCCTGTTGATTCCTTCACTTTTGTCTTCCCTATAATGGAGCGAATTCTTTTAAGCTCTAAGAAGACCGGGCTTCATGATAATGTGCTTCAGATTATTTACCTGCACATGGACCCCCTATTACCCCTCCCTAGGCTTCGAATGATTTCAGTTCTTTATCATGTTCTTGGTATTGTACAAGCTTATCAATCCTCTATTGGACCTGCGTTGAATGAGTTGTGTCTTGGTCTGCAGCCAGATGAAGTTGCACCGGCTTTATACGGGGTTTATGCAAAAGATGTTCATGTTAGAATGGCTTGTCTAACTGCTGTCAAATGCATTCCTACTGTTGCTAGTCGGTCTCTTATTCAGAATGTTGAGGTTGCTACAAGTATTTGGATTGCTTTACATGACCCAGAAAAGTCAGTTGCAGAAGCTGCAGAGGATTTGTGGGATCGCTATGGACATGACTTTGGGACTGACTATTCAGGCCTTTTCAAAGCACTTTCTCATATCCATTATACTGTCCGTTTTGCTGCTGCAGAGGCATTAGCTGCTGCTTTGGACGAAAATCCTGATTCTATACAGGAATCCCTTTCTACTTTATTCTCCCTGTATATCCGTGATGCTGGTTTGACTGAGAACAATGTCGATGCTGGTTGGCTGGGCCGGCAGGGTGTAGCATTGGCATTGCATTCTTCTGCTGATGTACTGAGAACAAAAGACCTTCCTGTTGTTATGACCTTTTTGATTTCAAGAGCATTGGCCGATCCTAATGCTGATGTTCGGGGGAGAATGATTAATGCTGGCATTATGATTATTGATAAGCACGGAAGAGATAACGTGTCCTTGCTATTTCCCATCTTTGAGAACTATTTAAACAAAAAGGCATCGGATGAGGAGACATATGATTTGGTTCGTGAGGGTGTTGTTATTTTCACTGGAGCGTTGGCAAAGCATTTAGCAAAGGATGATCCAAAAGTACATGCTGTTATAGAGAAGTTGTTGGATGTGTTGAACACTCCATCAGAAGCTGTTCAGCGGGCAGTTTCAACATGCCTATCACCATTAATGCAATCAAAGCAAGATGATGGCCAAGCTCTTGTTTCAAGGATTTTGGATCAATTGATGAACAGTGATAAGTATGGGGAACGCCGTGGAGCTGCTTTTGGGCTTGCTGGAGTAGTCAAGGGATTTGGTATTTCTTCCTTGAAGAAGTATGGAATTGTGAATATACTGCAGGAAGGACTTGTTGATAGGAATTCTGCAAAACGTCGTGAAGGGGGTTTGCTTGGATTTGAGTGTTTATGTGAAACACTTGGAAAATTATTTGAACCGTATGTAATACAAATGTTGCCACTACTGTTGGTTTCATTTTCTGATCAAGTTGTTGCAGTTCGTGAAGGTGCTGAGTGTGCTGCTCGGGCAATGATGTCTCACCTTACTGCTCAGGGAGTGAAACTGGTCCTTCCATCTCTGTTGAAGGGTCTTGAAGATAAAGCTTGGCGAACAAAGCAAAGTAGTGTTCAACTGCTTGGTGCTATGGCCTATTGTGCTCCTAAGCAGCTGTCTCAATGTCTCCCTAAGATTGTACCGAAACTGACTGAGGTTTTAACAGACACCCACCCTAAAGTTCAGTCAGCAGGACAAACAGCACTTCAACAGGTTGGGAGTGTAATAAAGAATCCTGAGATAGCTTCATTGGTCCCTACTCTTCTTCTGGGTCTTACAGATCCAAATGACTATACAAAATATTCTCTGGATGTTCTCTTGGGAACAACATTCATCAATTCAATTGATGCTCCTTCACTTGCACTCTTGGTGCCGATAGTACATAGAGGGCTAAGGGAGAGGGGTGCAGAGACTAAAAAGAAAGCAGCCCAAATAGTTGGCAATATGTGTTCGTTAGTTACAGATCCAAATGATATGATTCCGTACATAGGGCTGCTTCTTCCTGAAGTGAAAAAGGTTCTTGTGGATCCAATTCCAGAAGTTAGGTCTGTTGCTGCAAGGGCGCTTGGATCCCTCATAAGGGGAATGGGTGAAGATCATTTCCCAGATCTTGTTCCCTGGTTGTTGGATACACTTAAATCAGATAATAGTAATGTTGAGCGATCTGGAGCTGCTCAAGGGTTAAGTGAGGTATTAGCTGCACTTGGGACTGAGTACTTTGAGCATGTACTTCCTGATGTTATCCGAAATTGTTCTCATCAAAAAGCATCTGTGCGTGATGGGCATTTGACATTATTCAAGTATTTACCAAGGTCCTTAGGCGTCCAATTCCAGAATTATTTACAGCAGGTGCTGCCTGCTATTATAGATGGTCTTGCTGATGAAAATGAGTCTGTACGTGAAGCAGCATTAGGTGCGGGACATGTTCTGGTTGAGCATTATGCTATAACGTCGTTACCTTTGCTCCTACCCGCTGTTGAGGATGGTATCTTTAATGATAGTTGGCGAATTCGACAAAGCTCTGTGGAATTATTGGGGGATCTCTTATTTAAGGTTGCGGGTACTTCTGGAAAAGCTTTACTGGAGGGTGGCAGTGATGATGAAGGTGCAAGTACTGAAGCACAGGGGCGGGCTATCATTGAGGTTCTTGGAAGGGATAAGCGTGATGAAATTCTCGCTGCATTATATATGGTTCGAACTGATGTCAGCCTATCAGTGCGTCAGGCTGCGTTGCATGTATGGAAAACTATAGTGGCAAATACCCCAAAAACTCTAAAGGAAATAATGCCAGTTCTAATGAATACTCTAATTACCTCTCTTGCATCATCATCCTTTGAAAGGCGGCAGGTCGCTGCACGTGCACTGGGTGAGCTTGTCAGGAAGCTTGGTGAAAGAGTGCTGCCATTGATCATCCCAATTTTATCCCAAGGATTGAAGGACTCTGACACCAGTAGACGACAAGGTGTATGCATTGGTCTGAGTGAAGTAATGGCATCTGCAGCTAAAAGTCATTTGTTGAGTTTCATGGATGAGCTCATTCCAACCATTCGAACTGCTCTTTCAGATAGCATGCCCGAAGTTCGTGAGTCTGCTGGAATAGCATTCAGCACTCTGTACAAGAATGCTGGAATGCAAGCGATTGATGAAATTGTTCCCAGTCTTTTGCATGCTTTGGAGGACGATCAAACTTCTGATACTGCACTTGATGGTCTCAAACAAATCTTAAGTGTCAGGATTACTGCAGTTTTACCCCACATTTTGCCCAAGCTTGTCCAGCTTCCACTAACTGCATTTAATGCACATGCTCTTGGAGCTGTAGCTGAGGTTGCTGGGCCTGGTCTTAACACTCATCTTGGTACCGTTCTTCCTGCTTTACTTACTTCAATGGGTGTTGATGCAAAGGATGTTCAAACATTGGCAAAGGAGGCTGCTGAAACTGTGGTCTTAGTAATAGATGAGGAAGGTGTTGAATATCTTACATCTGAGCTACTTAGAGCTGTCAGCGATAGTCAGGCTTCAATTAGAAGAAGTGCCGCGTATCTTATAGGCTATTTCTTCAAAAACAGTAAATTATATTTGGTTGATGAAGCGCCAAACATGATATCTACCTTGATTGTCTTGCTCAGTGATTCTGATTCGGCTACTGTGGCGGTATCTTGGGAAGCATTGTCAAGGGTTGTAAGTTCAGTCCCCAAGGAGGTACTTCCTTCATATATTAAATTAGTACGAGATGCTGTGTCGACTTCCAGAGATAAGGAGCGCAGGAAAAAGAAGGGAGGACCTATTGTCATACCTGGATTTTGTTTACCAAAAGCACTTCAACCGTTGCTTCCAATATTTCTCCAGGGTTTAATAAGTGGATCTGCTGAACTGAGAGAGCAAGCGGCACTGGGTCTTGGAGAACTGATTGAAGTGACTAGTGAAAAGGCACTCAAAGAGTTTGTCATTCCAATTACAGGGCCTCTTATTCGGATAATAGGTGACCGGTTCCCTTGGCAGGTGAAGAGTGCTATTTTATCTACCTTGACAATCATAATCAGAAAAGGTGGGATGGCCTTGAAACCATTTCTTCCGCAGCTTCAAACAACATTTGTCAAGTGTCTACAGGATAACACAAGAATTGTTCGCTCAAGTGCTGCCCTTGCTCTCGGAAAGCTTAGTGCTTTAAGTACCCGGGTGGACCCTCTAGTTGGAGACCTCCTGTCCAGTCTGCAGGCTATGGATAGTGGGGTTCGGGAGGCAAGTTTGTCCGCTTTAGAAGGTGTCCTAAAGCATGCTGGAAAGAGCGTGAGCAGTGCTGTTAGAACTCGTGTCTACTTGCAGCTTAAGGATTTGATTCATCATGATGATGATGAGGTTCGAGTATCTGCTGCAAGCATTTTGGGTATCATGTCCCAGTACATTGAAGATACCCAGCTGATCGAGCTGCTTCAGGAACTATCAAGTTTTCCATCGTCGCTCAGCTGGACTGCTAGGCATGGTTCTGTACTTACAATTTCATCCATGCTCAGGCACATCCCATCCACCATCTGTACTTCTCCAGTGTTTCCGTCTATCCTGGACCAGCTTAAAGCTGCCTTGACTGATGAGAAGTTCCCTCTTCGTGAAACTTCAACCAAGGCATTTGGGAGACTTCTTGTTTATAAAGTCCGAAGTGATCCTTCTAATACCTCAGTACAATTAGATATTATATCGTCTTTAGTCTCAGCATTGCATGATGATTCTAGCGAAGTTAGAAGGAAGGCGTTATCTGCTCTAAAGGCAGTTTCGAAGGAAAGTTGTTCATCTATTGCGGCCCATATCAATATTATTGGACCTGCACTGGCTGAATGTTTGAAGGATGGAAGTACACCCGTGAGACTTGCAGCAGAAAGATGTGCTCTGCATGCCTTCCAATTGACAAAGGGTCCTGAAAATGTTCAAGCTGCACAAAAGTTCATCACAGGCTTGGAAGCTAGACGAATATCAAAGTTTTCAGAACATAGTGATGATAGTGAAGGTGAGGATGACTTGGCAAGTGGCTGA

Protein Analysis

2621

Amino Acids

286.76

Weight (kDa)

6.58

Isoelectric Point (pI)

42.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GNC1_N PF24993 211 - 344 1.6e-45 Stalled ribosome sensor GCN1 N-terminal
HEAT_EF3_N PF24987 1348 - 1515 1.4e-46 EF3 N-terminal HEAT repeat
HEAT_GCN1 PF23271 1398 - 1535 2.7e-10 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_GNC1 PF24984 1553 - 1639 5.8e-26 Elongation factor 3/GNC1 HEAT repeat
HEAT_MROH2B_C PF23227 1616 - 1750 1e-05 MROH2B-like, HEAT repeats
HEAT_GCN1 PF23271 1627 - 1774 2e-11 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_N PF24987 1668 - 1797 2.7e-08 EF3 N-terminal HEAT repeat
TPR_DNAAF5 PF25757 1836 - 1992 8e-07 Dynein axonemal assembly factor 5-like, TPR repeats
HEAT_GCN1 PF23271 1870 - 2016 4.6e-07 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_N PF24987 1883 - 1978 1.5e-07 EF3 N-terminal HEAT repeat
HEAT_EF3_GNC1 PF24984 1906 - 1985 6.2e-06 Elongation factor 3/GNC1 HEAT repeat
HEAT_GCN1 PF23271 2029 - 2218 4e-52 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_MROH2B_C PF23227 2312 - 2422 4.9e-07 MROH2B-like, HEAT repeats
HEAT_GCN1_C_2 PF25801 2341 - 2592 4.4e-31 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_GCN1_C PF25786 2439 - 2593 2.3e-74 GCN1-like, C-terminal HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1716, 7485
AarI CACCTGC 2 cut(s) 5179, 6829
AasI GACNNNNNNGTC 1 cut(s) 1789
Acc36I ACCTGC 7 cut(s) 1933, 3021, 3121, 5179, 5677, 6829, 7666
Acc65I GGTACC 1 cut(s) 6197
AccB1I GGYRCC 2 cut(s) 4711, 6197
AccI GTMKAC 8 cut(s) 1309, 2316, 2456, 2626, 5794, 6581, 6943, 7155
AccII CGCG 1 cut(s) 6377
AcoI YGGCCR 5 cut(s) 96, 313, 2595, 3654, 3982
AcuI CTGAAG 7 cut(s) 1289, 2307, 2805, 2984, 4866, 5478, 7256
AcvI CACGTG 2 cut(s) 2501, 5699
AcyI GRCGYC 2 cut(s) 2313, 5163
AdeI CACNNNGTG 1 cut(s) 5708
AflII CTTAAG 3 cut(s) 2513, 6064, 7166
AflIII ACRYGT 3 cut(s) 304, 3732, 5263
AgeI ACCGGT 1 cut(s) 6825
AhdI GACNNNNNGTC 3 cut(s) 6626, 7042, 7746
AhlI ACTAGT 1 cut(s) 6758
AjnI CCWGG 7 cut(s) 2182, 2637, 4958, 6175, 6634, 6684, 7398
AjuI GAANNNNNNNTTGG 2 cut(s) 368, 400
Alw21I GWGCWC 5 cut(s) 928, 4447, 5704, 5877, 7723
Alw44I GTGCAC 2 cut(s) 924, 5700
AlwNI CAGNNNCTG 5 cut(s) 496, 1337, 3326, 3923, 5194
Ama87I CYCGRG 2 cut(s) 4311, 7014
ApaLI GTGCAC 2 cut(s) 924, 5700
ArsI GACNNNNNNTTYG 4 cut(s) 3784, 3816, 6768, 6800
AseI ATTAAT 3 cut(s) 2877, 3690, 3959
AsiGI ACCGGT 1 cut(s) 6825
Asp700I GAANNNNTTC 8 cut(s) 383, 708, 744, 3055, 3924, 5092, 5991, 7758
Asp718I GGTACC 1 cut(s) 6197
AspA2I CCTAGG 1 cut(s) 3041
AspLEI GCGC 4 cut(s) 2772, 4906, 6433, 6603
AsuC2I CCSGG 4 cut(s) 37, 2980, 7015, 7016
AsuII TTCGAA 5 cut(s) 1702, 3049, 5536, 5890, 7610
AvaI CYCGRG 2 cut(s) 4311, 7014
AvrII CCTAGG 1 cut(s) 3041
AxyI CCTNAGG 1 cut(s) 5158
BaeGI GKGCMC 2 cut(s) 928, 5704
BalI TGGCCA 3 cut(s) 315, 2597, 3984
BamHI GGATCC 2 cut(s) 4867, 4910
BanI GGYRCC 2 cut(s) 4711, 6197
BanII GRGCYC 1 cut(s) 5877
BarI GAAGNNNNNNTAC 2 cut(s) 1851, 1883
BauI CACGAG 1 cut(s) 7149
BbrPI CACGTG 2 cut(s) 2501, 5699
BbsI GAAGAC 7 cut(s) 332, 473, 1033, 1251, 2789, 2929, 2981
Bbv12I GWGCWC 5 cut(s) 928, 4447, 5704, 5877, 7723
BbvCI CCTCAGC 1 cut(s) 6162
BceAI ACGGC 3 cut(s) 124, 2349, 4029
BcgI CGANNNNNNTGC 4 cut(s) 1302, 1336, 3647, 3681
BciT130I CCWGG 7 cut(s) 2184, 2639, 4960, 6177, 6636, 6686, 7400
BciVI GTATCC 1 cut(s) 4963
BclI TGATCA 3 cut(s) 1059, 4270, 5748
BcnI CCSGG 4 cut(s) 37, 2980, 7015, 7016
BcuI ACTAGT 1 cut(s) 6758
BfoI RGCGCY 2 cut(s) 4907, 6434
BfrI CTTAAG 3 cut(s) 2513, 6064, 7166
BfuAI ACCTGC 7 cut(s) 1933, 3021, 3121, 5179, 5677, 6829, 7666
BfuI GTATCC 1 cut(s) 4963
BglII AGATCT 3 cut(s) 1217, 1497, 4948
BlnI CCTAGG 1 cut(s) 3041
BlpI GCTNAGC 1 cut(s) 7301
BmcAI AGTACT 2 cut(s) 5057, 5455
BmeRI GACNNNNNGTC 3 cut(s) 6626, 7042, 7746
BmeT110I CYCGRG 2 cut(s) 4311, 7014
BmrI ACTGGG 5 cut(s) 2457, 5714, 5991, 6740, 7231
BmuI ACTGGG 5 cut(s) 2457, 5714, 5991, 6740, 7231
BpiI GAAGAC 7 cut(s) 332, 473, 1033, 1251, 2789, 2929, 2981
BpmI CTGGAG 8 cut(s) 1074, 2205, 3852, 4089, 5028, 5444, 6668, 7365
Bpu10I CCTNAGC 6 cut(s) 2358, 4338, 4449, 4733, 6162, 7349
Bpu1102I GCTNAGC 1 cut(s) 7301
Bpu14I TTCGAA 5 cut(s) 1702, 3049, 5536, 5890, 7610
BpuEI CTTGAG 5 cut(s) 574, 583, 641, 5001, 6955
BpuMI CCSGG 4 cut(s) 37, 2980, 7015, 7016
BsaAI YACGTR 4 cut(s) 2501, 2534, 5240, 5699
BsaBI GATNNNNATC 1 cut(s) 651
BsaHI GRCGYC 2 cut(s) 2313, 5163
BsaI GGTCTC 5 cut(s) 49, 1472, 3255, 6056, 7029
BsaWI WCCGGW 1 cut(s) 6825
BsaXI ACNNNNNCTCC 6 cut(s) 907, 937, 2069, 2099, 5289, 5319
Bse118I RCCGGY 3 cut(s) 3160, 3558, 6825
Bse21I CCTNAGG 1 cut(s) 5158
Bse3DI GCAATG 8 cut(s) 990, 1171, 2175, 2598, 2754, 3578, 4323, 7550
Bse8I GATNNNNATC 1 cut(s) 651
BseBI CCWGG 7 cut(s) 2184, 2639, 4960, 6177, 6636, 6686, 7400
BseJI GATNNNNATC 1 cut(s) 651
BseMI GCAATG 8 cut(s) 990, 1171, 2175, 2598, 2754, 3578, 4323, 7550
BseRI GAGGAG 8 cut(s) 536, 1184, 1490, 1817, 2026, 2507, 2534, 3803
BseSI GKGCMC 2 cut(s) 928, 5704
BseYI CCCAGC 3 cut(s) 3553, 6170, 7254
BsgI GTGCAG 8 cut(s) 425, 2999, 4767, 5025, 5679, 6024, 7644, 7752
Bsh1236I CGCG 1 cut(s) 6377
BshNI GGYRCC 2 cut(s) 4711, 6197
BshTI ACCGGT 1 cut(s) 6825
BsiHKAI GWGCWC 5 cut(s) 928, 4447, 5704, 5877, 7723
BsiHKCI CYCGRG 2 cut(s) 4311, 7014
BsiSI CCGG 8 cut(s) 36, 95, 913, 2979, 3161, 3559, 6826, 7015
BslFI GGGAC 7 cut(s) 2919, 3379, 4580, 5062, 5274, 6515, 7219
BsmFI GGGAC 7 cut(s) 2919, 3379, 4580, 5062, 5274, 6515, 7219
BsmI GAATGC 8 cut(s) 383, 385, 819, 3228, 3583, 5942, 5968, 5976
Bso31I GGTCTC 5 cut(s) 49, 1472, 3255, 6056, 7029
BsoBI CYCGRG 2 cut(s) 4311, 7014
Bsp119I TTCGAA 5 cut(s) 1702, 3049, 5536, 5890, 7610
Bsp1286I GDGCHC 5 cut(s) 928, 4447, 5704, 5877, 7723
Bsp1407I TGTACA 4 cut(s) 168, 430, 3085, 5955
Bsp1720I GCTNAGC 1 cut(s) 7301
BspFNI CGCG 1 cut(s) 6377
BspHI TCATGA 3 cut(s) 243, 2986, 7183
BspMAI CTGCAG 8 cut(s) 1930, 3144, 3331, 3433, 4131, 5842, 6085, 7056
BspMI ACCTGC 7 cut(s) 1933, 3021, 3121, 5179, 5677, 6829, 7666
BspQI GCTCTTC 1 cut(s) 27
BspT104I TTCGAA 5 cut(s) 1702, 3049, 5536, 5890, 7610
BspT107I GGYRCC 2 cut(s) 4711, 6197
BspTI CTTAAG 3 cut(s) 2513, 6064, 7166
BspTNI GGTCTC 5 cut(s) 49, 1472, 3255, 6056, 7029
BsrDI GCAATG 8 cut(s) 990, 1171, 2175, 2598, 2754, 3578, 4323, 7550
BsrFI RCCGGY 3 cut(s) 3160, 3558, 6825
BsrGI TGTACA 4 cut(s) 168, 430, 3085, 5955
BssAI RCCGGY 3 cut(s) 3160, 3558, 6825
BssNI GRCGYC 2 cut(s) 2313, 5163
BssSI CACGAG 1 cut(s) 7149
BssT1I CCWWGG 8 cut(s) 1009, 1545, 3041, 5150, 5767, 6534, 6833, 7458
Bst2BI CACGAG 1 cut(s) 7149
Bst2UI CCWGG 7 cut(s) 2184, 2639, 4960, 6177, 6636, 6686, 7400
Bst6I CTCTTC 7 cut(s) 27, 1285, 2916, 3718, 4608, 6839, 7446
BstACI GRCGYC 2 cut(s) 2313, 5163
BstAFI CTTAAG 3 cut(s) 2513, 6064, 7166
BstAPI GCANNNNNTGC 7 cut(s) 733, 1220, 3326, 3443, 5194, 5255, 6137
BstAUI TGTACA 4 cut(s) 168, 430, 3085, 5955
BstBAI YACGTR 4 cut(s) 2501, 2534, 5240, 5699
BstBI TTCGAA 5 cut(s) 1702, 3049, 5536, 5890, 7610
BstDSI CCRYGG 1 cut(s) 4045
BstEII GGTNACC 2 cut(s) 2667, 6821
BstENI CCTNNNNNAGG 1 cut(s) 5677
BstFNI CGCG 1 cut(s) 6377
BstH2I RGCGCY 2 cut(s) 4907, 6434
BstHHI GCGC 4 cut(s) 2772, 4906, 6433, 6603
BstNI CCWGG 7 cut(s) 2184, 2639, 4960, 6177, 6636, 6686, 7400
BstPI GGTNACC 2 cut(s) 2667, 6821
BstSLI GKGCMC 2 cut(s) 928, 5704
BstUI CGCG 1 cut(s) 6377
BstV2I GAAGAC 7 cut(s) 332, 473, 1033, 1251, 2789, 2929, 2981
BstXI CCANNNNNNTGG 2 cut(s) 1052, 4778
Bsu36I CCTNAGG 1 cut(s) 5158
BsuI GTATCC 1 cut(s) 4963
BtgI CCRYGG 1 cut(s) 4045
BtsI GCAGTG 3 cut(s) 1067, 5440, 7141
BveI ACCTGC 7 cut(s) 1933, 3021, 3121, 5179, 5677, 6829, 7666
CaiI CAGNNNCTG 5 cut(s) 496, 1337, 3326, 3923, 5194
CciI TCATGA 3 cut(s) 243, 2986, 7183
CfoI GCGC 4 cut(s) 2772, 4906, 6433, 6603
Cfr10I RCCGGY 3 cut(s) 3160, 3558, 6825
Cfr9I CCCGGG 1 cut(s) 7014
CseI GACGC 3 cut(s) 2302, 5152, 5549
CspAI ACCGGT 1 cut(s) 6825
CspCI CAANNNNNGTGG 4 cut(s) 1673, 1708, 4236, 4271
DraI TTTAAA 3 cut(s) 160, 669, 3768
DraIII CACNNNGTG 1 cut(s) 5708
DrdI GACNNNNNNGTC 1 cut(s) 1789
DriI GACNNNNNGTC 3 cut(s) 6626, 7042, 7746
DseDI GACNNNNNNGTC 1 cut(s) 1789
EaeI YGGCCR 5 cut(s) 96, 313, 2595, 3654, 3982
Eam1104I CTCTTC 7 cut(s) 27, 1285, 2916, 3718, 4608, 6839, 7446
Eam1105I GACNNNNNGTC 3 cut(s) 6626, 7042, 7746
EarI CTCTTC 7 cut(s) 27, 1285, 2916, 3718, 4608, 6839, 7446
Ecl136II GAGCTC 1 cut(s) 5875
Eco130I CCWWGG 8 cut(s) 1009, 1545, 3041, 5150, 5767, 6534, 6833, 7458
Eco147I AGGCCT 1 cut(s) 3381
Eco24I GRGCYC 1 cut(s) 5877
Eco31I GGTCTC 5 cut(s) 49, 1472, 3255, 6056, 7029
Eco32I GATATC 3 cut(s) 1512, 2814, 6444
Eco53kI GAGCTC 1 cut(s) 5875
Eco57I CTGAAG 7 cut(s) 1289, 2307, 2805, 2984, 4866, 5478, 7256
Eco72I CACGTG 2 cut(s) 2501, 5699
Eco81I CCTNAGG 1 cut(s) 5158
Eco88I CYCGRG 2 cut(s) 4311, 7014
Eco91I GGTNACC 2 cut(s) 2667, 6821
EcoICRI GAGCTC 1 cut(s) 5875
EcoNI CCTNNNNNAGG 1 cut(s) 5677
EcoO109I RGGNCCY 8 cut(s) 2092, 2383, 2746, 2906, 5154, 6620, 6800, 7748
EcoO65I GGTNACC 2 cut(s) 2667, 6821
EcoRI GAATTC 5 cut(s) 584, 1775, 2955, 4150, 5349
EcoRII CCWGG 7 cut(s) 2182, 2637, 4958, 6175, 6634, 6684, 7398
EcoRV GATATC 3 cut(s) 1512, 2814, 6444
EcoT14I CCWWGG 8 cut(s) 1009, 1545, 3041, 5150, 5767, 6534, 6833, 7458
EcoT22I ATGCAT 3 cut(s) 385, 3230, 5813
EcoT38I GRGCYC 1 cut(s) 5877
ErhI CCWWGG 8 cut(s) 1009, 1545, 3041, 5150, 5767, 6534, 6833, 7458
FalI AAGNNNNNCTT 8 cut(s) 1696, 1728, 2280, 2312, 2781, 2813, 7486, 7518
FaqI GGGAC 7 cut(s) 2919, 3379, 4580, 5062, 5274, 6515, 7219
FauI CCCGC 6 cut(s) 60, 3924, 5251, 5320, 5392, 5463
FauNDI CATATG 3 cut(s) 729, 1319, 3796
FbaI TGATCA 3 cut(s) 1059, 4270, 5748
FblI GTMKAC 8 cut(s) 1309, 2316, 2456, 2626, 5794, 6581, 6943, 7155
FriOI GRGCYC 1 cut(s) 5877
GlaI GCGC 4 cut(s) 2771, 4905, 6432, 6602
GsaI CCCAGC 3 cut(s) 3557, 6174, 7258
GsuI CTGGAG 8 cut(s) 1074, 2205, 3852, 4089, 5028, 5444, 6668, 7365
HaeII RGCGCY 2 cut(s) 4907, 6434
HapII CCGG 8 cut(s) 36, 95, 913, 2979, 3161, 3559, 6826, 7015
HgaI GACGC 3 cut(s) 2302, 5152, 5549
HhaI GCGC 4 cut(s) 2772, 4906, 6433, 6603
Hin1I GRCGYC 2 cut(s) 2313, 5163
Hin6I GCGC 4 cut(s) 2770, 4904, 6431, 6601
HinP1I GCGC 4 cut(s) 2770, 4904, 6431, 6601
HincII GTYRAC 7 cut(s) 838, 1462, 1563, 2457, 2627, 2644, 6582
HindII GTYRAC 7 cut(s) 838, 1462, 1563, 2457, 2627, 2644, 6582
HindIII AAGCTT 8 cut(s) 705, 1178, 3090, 4389, 5415, 5723, 6107, 6995
HpaI GTTAAC 1 cut(s) 2644
HpaII CCGG 8 cut(s) 36, 95, 913, 2979, 3161, 3559, 6826, 7015
Hpy99I CGWCG 5 cut(s) 35, 110, 4167, 5296, 7300
HpyCH4IV ACGT 8 cut(s) 2192, 2500, 2533, 3732, 4162, 5239, 5291, 5698
HpySE526I ACGT 8 cut(s) 2192, 2500, 2533, 3732, 4162, 5239, 5291, 5698
Hsp92I GRCGYC 2 cut(s) 2313, 5163
HspAI GCGC 4 cut(s) 2770, 4904, 6431, 6601
KpnI GGTACC 1 cut(s) 6201
KroI GCCGGC 1 cut(s) 3558
KroNI GCCGGC 1 cut(s) 3560
Ksp22I TGATCA 3 cut(s) 1059, 4270, 5748
KspAI GTTAAC 1 cut(s) 2644
LguI GCTCTTC 1 cut(s) 27
MaeII ACGT 8 cut(s) 2192, 2500, 2533, 3732, 4162, 5239, 5291, 5698
MaeIII GTNAC 9 cut(s) 1116, 1148, 1656, 2667, 2779, 4798, 5295, 6754, 6821
MfeI CAATTG 3 cut(s) 4013, 4683, 7736
MhlI GDGCHC 5 cut(s) 928, 4447, 5704, 5877, 7723
MlsI TGGCCA 3 cut(s) 315, 2597, 3984
MluNI TGGCCA 3 cut(s) 315, 2597, 3984
MlyI GAGTC 7 cut(s) 137, 1844, 2336, 2866, 5240, 5774, 5936
MmeI TCCRAC 7 cut(s) 1611, 1963, 2784, 3876, 4947, 5906, 7012
Mox20I TGGCCA 3 cut(s) 315, 2597, 3984
Mph1103I ATGCAT 3 cut(s) 385, 3230, 5813
MroNI GCCGGC 1 cut(s) 3558
MroXI GAANNNNTTC 8 cut(s) 383, 708, 744, 3055, 3924, 5092, 5991, 7758
MscI TGGCCA 3 cut(s) 315, 2597, 3984
MslI CAYNNNNRTG 9 cut(s) 309, 903, 1607, 2037, 2168, 2991, 7185, 7188, 7827
Msp20I TGGCCA 3 cut(s) 315, 2597, 3984
MspA1I CMGCKG 5 cut(s) 3931, 4456, 5315, 7258, 7305
MspCI CTTAAG 3 cut(s) 2513, 6064, 7166
MspI CCGG 8 cut(s) 36, 95, 913, 2979, 3161, 3559, 6826, 7015
MunI CAATTG 3 cut(s) 4013, 4683, 7736
Mva1269I GAATGC 8 cut(s) 383, 385, 819, 3228, 3583, 5942, 5968, 5976
MvaI CCWGG 7 cut(s) 2184, 2639, 4960, 6177, 6636, 6686, 7400
MvnI CGCG 1 cut(s) 6377
NaeI GCCGGC 1 cut(s) 3560
NciI CCSGG 4 cut(s) 37, 2980, 7015, 7016
NdeI CATATG 3 cut(s) 729, 1319, 3796
NgoMIV GCCGGC 1 cut(s) 3558
NmeAIII GCCGAG 1 cut(s) 874
NmuCI GTSAC 6 cut(s) 1116, 1656, 2667, 2779, 6754, 6821
NsiI ATGCAT 3 cut(s) 385, 3230, 5813
NspV TTCGAA 5 cut(s) 1702, 3049, 5536, 5890, 7610
PaeI GCATGC 5 cut(s) 902, 5914, 6011, 7118, 7730
PagI TCATGA 3 cut(s) 243, 2986, 7183
PaqCI CACCTGC 2 cut(s) 5179, 6829
PceI AGGCCT 1 cut(s) 3381
PciI ACATGT 2 cut(s) 304, 5263
PciSI GCTCTTC 1 cut(s) 27
PcsI WCGNNNNNNNCGW 3 cut(s) 79, 111, 1309
PctI GAATGC 8 cut(s) 383, 385, 819, 3228, 3583, 5942, 5968, 5976
PdiI GCCGGC 1 cut(s) 3560
PdmI GAANNNNTTC 8 cut(s) 383, 708, 744, 3055, 3924, 5092, 5991, 7758
PfoI TCCNGGA 1 cut(s) 7398
PinAI ACCGGT 1 cut(s) 6825
PleI GAGTC 7 cut(s) 136, 1844, 2335, 2865, 5239, 5774, 5935
PmaCI CACGTG 2 cut(s) 2501, 5699
PmlI CACGTG 2 cut(s) 2501, 5699
PpsI GAGTC 7 cut(s) 136, 1844, 2335, 2865, 5239, 5774, 5935
Ppu21I YACGTR 4 cut(s) 2501, 2534, 5240, 5699
PpuMI RGGWCCY 5 cut(s) 2383, 2906, 5154, 6620, 7748
PscI ACATGT 2 cut(s) 304, 5263
PshBI ATTAAT 3 cut(s) 2877, 3690, 3959
PsiI TTATAA 2 cut(s) 1716, 7485
Psp124BI GAGCTC 1 cut(s) 5877
Psp5II RGGWCCY 5 cut(s) 2383, 2906, 5154, 6620, 7748
Psp6I CCWGG 7 cut(s) 2182, 2637, 4958, 6175, 6634, 6684, 7398
PspCI CACGTG 2 cut(s) 2501, 5699
PspEI GGTNACC 2 cut(s) 2667, 6821
PspFI CCCAGC 3 cut(s) 3553, 6170, 7254
PspGI CCWGG 7 cut(s) 2182, 2637, 4958, 6175, 6634, 6684, 7398
PspPPI RGGWCCY 5 cut(s) 2383, 2906, 5154, 6620, 7748
PstI CTGCAG 8 cut(s) 1930, 3144, 3331, 3433, 4131, 5842, 6085, 7056
PstNI CAGNNNCTG 5 cut(s) 496, 1337, 3326, 3923, 5194
PvuII CAGCTG 3 cut(s) 4456, 7258, 7305
RseI CAYNNNNRTG 9 cut(s) 309, 903, 1607, 2037, 2168, 2991, 7185, 7188, 7827
SacI GAGCTC 1 cut(s) 5877
SalI GTCGAC 3 cut(s) 2455, 2625, 6580
SapI GCTCTTC 1 cut(s) 27
ScaI AGTACT 2 cut(s) 5057, 5455
SchI GAGTC 7 cut(s) 137, 1844, 2336, 2866, 5240, 5774, 5936
SduI GDGCHC 5 cut(s) 928, 4447, 5704, 5877, 7723
SfuI TTCGAA 5 cut(s) 1702, 3049, 5536, 5890, 7610
SmaI CCCGGG 1 cut(s) 7016
SmiMI CAYNNNNRTG 9 cut(s) 309, 903, 1607, 2037, 2168, 2991, 7185, 7188, 7827
SmlI CTYRAG 8 cut(s) 589, 598, 656, 2513, 5016, 6064, 6970, 7166
SmoI CTYRAG 8 cut(s) 589, 598, 656, 2513, 5016, 6064, 6970, 7166
SpeI ACTAGT 1 cut(s) 6758
SphI GCATGC 5 cut(s) 902, 5914, 6011, 7118, 7730
SseBI AGGCCT 1 cut(s) 3381
SspI AATATT 7 cut(s) 156, 639, 1671, 2062, 4643, 6678, 7648
SstI GAGCTC 1 cut(s) 5877
StuI AGGCCT 1 cut(s) 3381
StyI CCWWGG 8 cut(s) 1009, 1545, 3041, 5150, 5767, 6534, 6833, 7458
TaiI ACGT 8 cut(s) 2195, 2503, 2536, 3735, 4165, 5242, 5294, 5701
TaqII GACCGA 2 cut(s) 31, 3237
TauI GCSGC 6 cut(s) 8, 1745, 5686, 6377, 6728, 7637
TseFI GTSAC 6 cut(s) 1116, 1656, 2667, 2779, 6754, 6821
Tsp45I GTSAC 6 cut(s) 1116, 1656, 2667, 2779, 6754, 6821
TspGWI ACGGA 6 cut(s) 19, 3407, 3500, 3735, 4812, 7380
TspMI CCCGGG 1 cut(s) 7014
Vha464I CTTAAG 3 cut(s) 2513, 6064, 7166
VneI GTGCAC 2 cut(s) 924, 5700
VspI ATTAAT 3 cut(s) 2877, 3690, 3959
XagI CCTNNNNNAGG 1 cut(s) 5677
XbaI TCTAGA 1 cut(s) 2714
XmaI CCCGGG 1 cut(s) 7014
XmaJI CCTAGG 1 cut(s) 3041
XmiI GTMKAC 8 cut(s) 1309, 2316, 2456, 2626, 5794, 6581, 6943, 7155
XmnI GAANNNNTTC 8 cut(s) 383, 708, 744, 3055, 3924, 5092, 5991, 7758
ZrmI AGTACT 2 cut(s) 5057, 5455
Zsp2I ATGCAT 3 cut(s) 385, 3230, 5813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.