AT1G65870

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003503
Physical Location & Seq
Forward (+)
24503315 .. 24504390
1076 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G65870.1

Sequence Viewer

Length: 570 bp
ATGGCTAGTCTTTATCTTCTACTACTTCTTCCTCTGTTCTTAGCTCTTATTCTCGCAGCCACCATTACAGAATCAAAATCTTTCTCAACAACAGTGAAGGCACCATATCCAGGACATAAACCGGATAAGCTAACCCACTTACACTTCTACTTCCACGACATTGTCTCCGGAGACAAACCAACCTCCGTCCAAGTCGCTAACGGTCCAACCACAAACTCTTCTGCCACCGGTTTCGGTTTGGTTGCAGTCGTTGACGACAAATTGACGGTCGGGCCTGAAATTACCTCAGAGGAAGTTGGGAGAGCCCAAGGAATGTATGCATCGGCTGACCAGAACAAGCTTGGTCTGCTTATGGCTTTTAACTTGGTTTTCACGAAGGGTAAATTTTCCGACAGCACCGTGGCGATGTATGGCCGGAATCCTGTATTGTCGAAGGTGAGGGAAATGCCGATCATTGGAGGTACGGGAGCTTTCAGGTTCGGAAGAGGATATGCATTGGCCAAGACTTTGGTGTTCAATATTACCAGTGGAGATGCTGTGGTCGAATATAATGTCTATATTTGGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook Dirigent

Protein Analysis

189

Amino Acids

20.4

Weight (kDa)

8.76

Isoelectric Point (pI)

26.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 45 - 187 1.6e-57 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 100
AccIII TCCGGA 1 cut(s) 167
AcoI YGGCCR 2 cut(s) 412, 498
AcsI RAATTY 1 cut(s) 383
AfaI GTAC 1 cut(s) 463
AfiI CCNNNNNNNGG 2 cut(s) 110, 455
AgeI ACCGGT 1 cut(s) 227
AgsI TTSAA 1 cut(s) 517
AjnI CCWGG 1 cut(s) 109
AluBI AGCT 4 cut(s) 44, 130, 340, 470
AluI AGCT 4 cut(s) 44, 130, 340, 470
Alw26I GTCTC 2 cut(s) 165, 169
Aor13HI TCCGGA 1 cut(s) 167
AoxI GGCC 3 cut(s) 272, 412, 498
ApeKI GCWGC 1 cut(s) 56
ApoI RAATTY 1 cut(s) 383
ArsI GACNNNNNNTTYG 4 cut(s) 252, 284, 537, 569
AsiGI ACCGGT 1 cut(s) 227
AspS9I GGNCC 2 cut(s) 203, 272
AsuHPI GGTGA 1 cut(s) 448
AvaII GGWCC 1 cut(s) 203
BalI TGGCCA 1 cut(s) 500
BanI GGYRCC 1 cut(s) 100
BanII GRGCYC 1 cut(s) 307
BbvI GCAGC 1 cut(s) 68
BciT130I CCWGG 1 cut(s) 111
BcoDI GTCTC 2 cut(s) 165, 169
BfaI CTAG 1 cut(s) 6
BisI GCNGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 58
Bme1390I CCNGG 1 cut(s) 111
Bme18I GGWCC 1 cut(s) 203
BmgT120I GGNCC 2 cut(s) 203, 272
BmiI GGNNCC 1 cut(s) 102
BmrFI CCNGG 1 cut(s) 111
BmsI GCATC 2 cut(s) 329, 523
BsaJI CCNNGG 2 cut(s) 307, 399
BsaWI WCCGGW 3 cut(s) 121, 167, 227
BsaXI ACNNNNNCTCC 4 cut(s) 149, 179, 522, 552
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 455
Bse118I RCCGGY 1 cut(s) 227
Bse1I ACTGG 1 cut(s) 525
BseAI TCCGGA 1 cut(s) 167
BseBI CCWGG 1 cut(s) 111
BseDI CCNNGG 2 cut(s) 307, 399
BseLI CCNNNNNNNGG 2 cut(s) 110, 455
BseMII CTCAG 1 cut(s) 300
BseNI ACTGG 1 cut(s) 525
BseXI GCAGC 1 cut(s) 68
Bsh1285I CGRYCG 1 cut(s) 270
BshFI GGCC 3 cut(s) 274, 414, 500
BshNI GGYRCC 1 cut(s) 100
BshTI ACCGGT 1 cut(s) 227
BsiEI CGRYCG 1 cut(s) 270
BsiSI CCGG 4 cut(s) 122, 168, 228, 415
BslI CCNNNNNNNGG 2 cut(s) 110, 455
BsmAI GTCTC 2 cut(s) 165, 169
BsnI GGCC 3 cut(s) 274, 414, 500
Bsp1286I GDGCHC 1 cut(s) 307
Bsp13I TCCGGA 1 cut(s) 167
Bsp143I GATC 1 cut(s) 450
BspANI GGCC 3 cut(s) 274, 414, 500
BspCNI CTCAG 1 cut(s) 299
BspEI TCCGGA 1 cut(s) 167
BspLI GGNNCC 1 cut(s) 102
BspT107I GGYRCC 1 cut(s) 100
BsrFI RCCGGY 1 cut(s) 227
BsrI ACTGG 1 cut(s) 525
BssAI RCCGGY 1 cut(s) 227
BssECI CCNNGG 2 cut(s) 307, 399
BssMI GATC 1 cut(s) 450
BssT1I CCWWGG 1 cut(s) 307
Bst2UI CCWGG 1 cut(s) 111
Bst4CI ACNGT 4 cut(s) 94, 203, 268, 400
Bst6I CTCTTC 2 cut(s) 223, 478
BstDEI CTNAG 2 cut(s) 40, 286
BstDSI CCRYGG 1 cut(s) 399
BstKTI GATC 1 cut(s) 453
BstMAI GTCTC 2 cut(s) 165, 169
BstMBI GATC 1 cut(s) 450
BstMCI CGRYCG 1 cut(s) 270
BstMWI GCNNNNNNNGC 1 cut(s) 346
BstNI CCWGG 1 cut(s) 111
BstSCI CCNGG 1 cut(s) 109
BstV1I GCAGC 1 cut(s) 68
BstXI CCANNNNNNTGG 1 cut(s) 508
BsuRI GGCC 3 cut(s) 274, 414, 500
BtgI CCRYGG 1 cut(s) 399
BtgZI GCGATG 1 cut(s) 419
BtsIMutI CAGTG 2 cut(s) 99, 532
Cfr10I RCCGGY 1 cut(s) 227
Cfr13I GGNCC 2 cut(s) 203, 272
Csp6I GTAC 1 cut(s) 462
CspAI ACCGGT 1 cut(s) 227
CviQI GTAC 1 cut(s) 462
DdeI CTNAG 2 cut(s) 40, 286
DpnI GATC 1 cut(s) 452
DpnII GATC 1 cut(s) 450
EaeI YGGCCR 2 cut(s) 412, 498
Eam1104I CTCTTC 2 cut(s) 223, 478
EarI CTCTTC 2 cut(s) 223, 478
Eco130I CCWWGG 1 cut(s) 307
Eco24I GRGCYC 1 cut(s) 307
Eco47I GGWCC 1 cut(s) 203
EcoRII CCWGG 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 307
EcoT22I ATGCAT 2 cut(s) 322, 496
EcoT38I GRGCYC 1 cut(s) 307
ErhI CCWWGG 1 cut(s) 307
FaiI YATR 8 cut(s) 106, 117, 318, 353, 411, 492, 549, 558
Fnu4HI GCNGC 1 cut(s) 57
FriOI GRGCYC 1 cut(s) 307
Fsp4HI GCNGC 1 cut(s) 57
FspBI CTAG 1 cut(s) 6
GluI GCNGC 1 cut(s) 57
HaeIII GGCC 3 cut(s) 274, 414, 500
HapII CCGG 4 cut(s) 122, 168, 228, 415
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 2 cut(s) 71, 418
HpaII CCGG 4 cut(s) 122, 168, 228, 415
HphI GGTGA 1 cut(s) 448
Hpy166II GTNNAC 1 cut(s) 253
Hpy188I TCNGA 3 cut(s) 289, 391, 482
Hpy188III TCNNGA 2 cut(s) 168, 373
Hpy8I GTNNAC 1 cut(s) 253
HpyAV CCTTC 3 cut(s) 91, 370, 427
HpyCH4III ACNGT 4 cut(s) 94, 203, 268, 400
HpyCH4V TGCA 3 cut(s) 245, 320, 494
HpyF10VI GCNNNNNNNGC 1 cut(s) 346
HpyF3I CTNAG 2 cut(s) 40, 286
Kpn2I TCCGGA 1 cut(s) 167
Kzo9I GATC 1 cut(s) 450
LmnI GCTCC 1 cut(s) 467
Lsp1109I GCAGC 1 cut(s) 68
LweI GCATC 2 cut(s) 329, 523
MaeI CTAG 1 cut(s) 6
MalI GATC 1 cut(s) 452
MboI GATC 1 cut(s) 450
MboII GAAGA 4 cut(s) 8, 20, 210, 495
MhlI GDGCHC 1 cut(s) 307
MlsI TGGCCA 1 cut(s) 500
MluCI AATT 3 cut(s) 260, 279, 383
MluNI TGGCCA 1 cut(s) 500
MmeI TCCRAC 2 cut(s) 230, 414
MnlI CCTC 7 cut(s) 42, 193, 283, 295, 432, 452, 479
Mox20I TGGCCA 1 cut(s) 500
Mph1103I ATGCAT 2 cut(s) 322, 496
MroI TCCGGA 1 cut(s) 167
MscI TGGCCA 1 cut(s) 500
MseI TTAA 2 cut(s) 360, 568
Msp20I TGGCCA 1 cut(s) 500
MspI CCGG 4 cut(s) 122, 168, 228, 415
MspR9I CCNGG 1 cut(s) 111
MvaI CCWGG 1 cut(s) 111
MwoI GCNNNNNNNGC 1 cut(s) 346
NdeII GATC 1 cut(s) 450
NlaIV GGNNCC 1 cut(s) 102
NsiI ATGCAT 2 cut(s) 322, 496
PfeI GAWTC 2 cut(s) 71, 418
PflFI GACNNNGTC 1 cut(s) 161
PfoI TCCNGGA 1 cut(s) 109
PinAI ACCGGT 1 cut(s) 227
PkrI GCNGC 1 cut(s) 58
Psp6I CCWGG 1 cut(s) 109
PspGI CCWGG 1 cut(s) 109
PspN4I GGNNCC 1 cut(s) 102
PspPI GGNCC 2 cut(s) 203, 272
PsyI GACNNNGTC 1 cut(s) 161
RsaI GTAC 1 cut(s) 463
RsaNI GTAC 1 cut(s) 462
SaqAI TTAA 2 cut(s) 360, 568
SatI GCNGC 1 cut(s) 57
Sau3AI GATC 1 cut(s) 450
Sau96I GGNCC 2 cut(s) 203, 272
ScrFI CCNGG 1 cut(s) 111
SduI GDGCHC 1 cut(s) 307
SetI ASST 9 cut(s) 46, 132, 185, 287, 342, 438, 463, 472, 479
SfaNI GCATC 2 cut(s) 329, 523
SinI GGWCC 1 cut(s) 203
Sse9I AATT 3 cut(s) 260, 279, 383
SspI AATATT 1 cut(s) 520
SspMI CTAG 1 cut(s) 6
StyD4I CCNGG 1 cut(s) 109
StyI CCWWGG 1 cut(s) 307
TaaI ACNGT 4 cut(s) 94, 203, 268, 400
TaqI TCGA 2 cut(s) 431, 543
TasI AATT 3 cut(s) 260, 279, 383
TfiI GAWTC 2 cut(s) 71, 418
Tru1I TTAA 2 cut(s) 360, 568
Tru9I TTAA 2 cut(s) 360, 568
TscAI CASTG 2 cut(s) 99, 532
TseI GCWGC 1 cut(s) 56
TspGWI ACGGA 1 cut(s) 175
TspRI CASTG 2 cut(s) 99, 532
Tth111I GACNNNGTC 1 cut(s) 161
VpaK11BI GGWCC 1 cut(s) 203
XapI RAATTY 1 cut(s) 383
XcmI CCANNNNNNNNNTGG 1 cut(s) 338
XspI CTAG 1 cut(s) 6
Zsp2I ATGCAT 2 cut(s) 322, 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.