MD12G1053900.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003503
Physical Location & Seq
Forward (+)
6008878 .. 6009468
591 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1053900.v1.1.491

Sequence Viewer

Length: 591 bp
ATGGTCAAGAACCTCAAAAACCTCAGTTCTACCTACACCATTCTCATAACCATTTCCTTCTTCTTCTTCTTAACCCTTGTCACTTCAGAGACTCACCATTTCTCAAGACCCTTATCTCCATCAAAACATGGTCTAAAGAGAGAGAAACTGAGCCATCTGCACTTCTACTTCCATGACATAGTAGGTGGCCGAACCCCCACGGCAATTAAAGTGGCTGAGGCACTCACGACAAACACTTCCATGACTGGCTTTGGCACCGTGGTCATGATGGACGATCCATTGACTATCGGCCCTGAATCGAGCTCTAAGCTAGTCGGAAAAGCCCAAGGTATTTATGCTTCAGCTTCACAAAGTGAGGTGGGCTTTTTGATGGCTTTGAACTTTGTATTTGTTGAAGGGAAGTATAATGGTAGCACTCTCAGTGTGTTGGGGCGCAACGCCGTGTTCTCAGCTGTGAGGGAGATGCCGATAGTAGGAGGAAGTGGGCTTTTCCGATTTGCTCGTGGCTATGCTCATGCTAGCACTCATCAGTTTGACATCAAAACTGGAGATGCTGTTGTGGAGTATAATGTTTATGTCTTCCATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

197

Amino Acids

21.53

Weight (kDa)

9.3

Isoelectric Point (pI)

23.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 51 - 194 3.6e-60 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 254
AclWI GGATC 1 cut(s) 269
AcoI YGGCCR 1 cut(s) 187
AcuI CTGAAG 2 cut(s) 69, 324
AdeI CACNNNGTG 1 cut(s) 353
AfiI CCNNNNNNNGG 1 cut(s) 473
AgsI TTSAA 2 cut(s) 379, 395
AluBI AGCT 4 cut(s) 303, 310, 344, 452
AluI AGCT 4 cut(s) 303, 310, 344, 452
Alw21I GWGCWC 1 cut(s) 305
Alw26I GTCTC 1 cut(s) 83
AlwI GGATC 1 cut(s) 269
AoxI GGCC 2 cut(s) 187, 289
AspLEI GCGC 1 cut(s) 435
AspS9I GGNCC 1 cut(s) 290
AsuHPI GGTGA 1 cut(s) 86
AsuNHI GCTAGC 1 cut(s) 518
BanI GGYRCC 1 cut(s) 254
BanII GRGCYC 1 cut(s) 305
BarI GAAGNNNNNNTAC 2 cut(s) 322, 354
BauI CACGAG 1 cut(s) 501
BbsI GAAGAC 1 cut(s) 571
Bbv12I GWGCWC 1 cut(s) 305
BbvCI CCTCAGC 1 cut(s) 216
BccI CCATC 4 cut(s) 127, 162, 262, 364
BceAI ACGGC 2 cut(s) 216, 425
BcoDI GTCTC 1 cut(s) 83
BfaI CTAG 2 cut(s) 311, 519
BmgT120I GGNCC 1 cut(s) 290
BmiI GGNNCC 1 cut(s) 256
BmsI GCATC 2 cut(s) 453, 541
BmtI GCTAGC 1 cut(s) 522
BpiI GAAGAC 1 cut(s) 571
BpmI CTGGAG 1 cut(s) 567
Bpu10I CCTNAGC 1 cut(s) 216
BpuEI CTTGAG 1 cut(s) 88
BsaJI CCNNGG 3 cut(s) 198, 258, 325
BsaXI ACNNNNNCTCC 2 cut(s) 540, 570
Bsc4I CCNNNNNNNGG 1 cut(s) 473
Bse1I ACTGG 2 cut(s) 250, 550
BseDI CCNNGG 3 cut(s) 198, 258, 325
BseLI CCNNNNNNNGG 1 cut(s) 473
BseMII CTCAG 5 cut(s) 37, 140, 207, 433, 462
BseNI ACTGG 2 cut(s) 250, 550
BsgI GTGCAG 1 cut(s) 143
BshFI GGCC 2 cut(s) 189, 291
BshNI GGYRCC 1 cut(s) 254
BsiHKAI GWGCWC 1 cut(s) 305
BslI CCNNNNNNNGG 1 cut(s) 473
BsmAI GTCTC 1 cut(s) 83
BsnI GGCC 2 cut(s) 189, 291
Bsp1286I GDGCHC 1 cut(s) 305
Bsp143I GATC 1 cut(s) 274
BspANI GGCC 2 cut(s) 189, 291
BspCNI CTCAG 5 cut(s) 36, 141, 208, 432, 461
BspHI TCATGA 1 cut(s) 264
BspLI GGNNCC 1 cut(s) 256
BspOI GCTAGC 1 cut(s) 522
BspPI GGATC 1 cut(s) 269
BspT107I GGYRCC 1 cut(s) 254
BsrI ACTGG 2 cut(s) 250, 550
BssECI CCNNGG 3 cut(s) 198, 258, 325
BssMI GATC 1 cut(s) 274
BssSI CACGAG 1 cut(s) 501
BssT1I CCWWGG 1 cut(s) 325
Bst2BI CACGAG 1 cut(s) 501
Bst4CI ACNGT 1 cut(s) 259
BstC8I GCNNGC 1 cut(s) 520
BstDEI CTNAG 6 cut(s) 23, 149, 216, 306, 419, 448
BstDSI CCRYGG 2 cut(s) 198, 258
BstHHI GCGC 1 cut(s) 435
BstKTI GATC 1 cut(s) 277
BstMAI GTCTC 1 cut(s) 83
BstMBI GATC 1 cut(s) 274
BstV2I GAAGAC 1 cut(s) 571
BsuRI GGCC 2 cut(s) 189, 291
BtgI CCRYGG 2 cut(s) 198, 258
BtsIMutI CAGTG 1 cut(s) 427
Cac8I GCNNGC 1 cut(s) 520
CciI TCATGA 1 cut(s) 264
CfoI GCGC 1 cut(s) 435
Cfr13I GGNCC 1 cut(s) 290
CspCI CAANNNNNGTGG 2 cut(s) 192, 227
CviAII CATG 5 cut(s) 128, 173, 241, 265, 515
DdeI CTNAG 6 cut(s) 23, 149, 216, 306, 419, 448
DpnI GATC 1 cut(s) 276
DpnII GATC 1 cut(s) 274
DraIII CACNNNGTG 1 cut(s) 353
EaeI YGGCCR 1 cut(s) 187
Ecl136II GAGCTC 1 cut(s) 303
Eco130I CCWWGG 1 cut(s) 325
Eco24I GRGCYC 1 cut(s) 305
Eco53kI GAGCTC 1 cut(s) 303
Eco57I CTGAAG 2 cut(s) 69, 324
EcoICRI GAGCTC 1 cut(s) 303
EcoT14I CCWWGG 1 cut(s) 325
EcoT38I GRGCYC 1 cut(s) 305
ErhI CCWWGG 1 cut(s) 325
FaeI CATG 5 cut(s) 131, 176, 244, 268, 518
FatI CATG 5 cut(s) 127, 172, 240, 264, 514
FriOI GRGCYC 1 cut(s) 305
FspBI CTAG 2 cut(s) 311, 519
GlaI GCGC 1 cut(s) 434
GsuI CTGGAG 1 cut(s) 567
HaeIII GGCC 2 cut(s) 189, 291
HhaI GCGC 1 cut(s) 435
Hin1II CATG 5 cut(s) 131, 176, 244, 268, 518
Hin6I GCGC 1 cut(s) 433
HinP1I GCGC 1 cut(s) 433
HinfI GANTC 2 cut(s) 91, 296
HphI GGTGA 1 cut(s) 86
Hpy188I TCNGA 3 cut(s) 88, 317, 494
Hpy188III TCNNGA 4 cut(s) 7, 105, 226, 265
HpyAV CCTTC 2 cut(s) 67, 389
HpyCH4III ACNGT 1 cut(s) 259
HpyCH4V TGCA 1 cut(s) 160
HpyF3I CTNAG 6 cut(s) 23, 149, 216, 306, 419, 448
Hsp92II CATG 5 cut(s) 131, 176, 244, 268, 518
HspAI GCGC 1 cut(s) 433
Kzo9I GATC 1 cut(s) 274
LpnPI CCDG 3 cut(s) 231, 306, 531
LweI GCATC 2 cut(s) 453, 541
MaeI CTAG 2 cut(s) 311, 519
MaeIII GTNAC 1 cut(s) 79
MalI GATC 1 cut(s) 276
MboI GATC 1 cut(s) 274
MboII GAAGA 4 cut(s) 52, 55, 58, 571
MhlI GDGCHC 1 cut(s) 305
MluCI AATT 1 cut(s) 204
MlyI GAGTC 1 cut(s) 85
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 6 cut(s) 23, 32, 211, 349, 450, 470
MseI TTAA 3 cut(s) 71, 207, 589
MslI CAYNNNNRTG 1 cut(s) 239
MspA1I CMGCKG 1 cut(s) 452
NdeII GATC 1 cut(s) 274
NheI GCTAGC 1 cut(s) 518
NlaIII CATG 5 cut(s) 131, 176, 244, 268, 518
NlaIV GGNNCC 1 cut(s) 256
NmuCI GTSAC 1 cut(s) 79
PagI TCATGA 1 cut(s) 264
PfeI GAWTC 1 cut(s) 296
PleI GAGTC 1 cut(s) 85
PpsI GAGTC 1 cut(s) 85
Psp124BI GAGCTC 1 cut(s) 305
PspN4I GGNNCC 1 cut(s) 256
PspPI GGNCC 1 cut(s) 290
PvuII CAGCTG 1 cut(s) 452
RseI CAYNNNNRTG 1 cut(s) 239
SacI GAGCTC 1 cut(s) 305
SaqAI TTAA 3 cut(s) 71, 207, 589
Sau3AI GATC 1 cut(s) 274
Sau96I GGNCC 1 cut(s) 290
SchI GAGTC 1 cut(s) 85
SduI GDGCHC 1 cut(s) 305
SfaNI GCATC 2 cut(s) 453, 541
SmiMI CAYNNNNRTG 1 cut(s) 239
SmlI CTYRAG 1 cut(s) 103
SmoI CTYRAG 1 cut(s) 103
Sse9I AATT 1 cut(s) 204
SspMI CTAG 2 cut(s) 311, 519
SstI GAGCTC 1 cut(s) 305
StyI CCWWGG 1 cut(s) 325
TaaI ACNGT 1 cut(s) 259
TaqI TCGA 1 cut(s) 299
TasI AATT 1 cut(s) 204
TfiI GAWTC 1 cut(s) 296
Tru1I TTAA 3 cut(s) 71, 207, 589
Tru9I TTAA 3 cut(s) 71, 207, 589
TscAI CASTG 1 cut(s) 427
TseFI GTSAC 1 cut(s) 79
Tsp45I GTSAC 1 cut(s) 79
TspRI CASTG 1 cut(s) 427
XspI CTAG 2 cut(s) 311, 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.