RchiOBHm_Chr3g0479011

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0003503
Physical Location & Seq
Forward (+)
24685255 .. 24686254
1000 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44414

Sequence Viewer

Length: 513 bp
ATGCCACTGCAAAATCCTCAATCTTCTCAGAAAACACCTCCATTGACACTTGGGATCAAGCAAGAGAAGCTAAGCCACCTCCACTTCTACTTCCACGATATCGCCAGCGGTCCCAACCCAACTTCCATCTGGGTTGCCCGAGCACCCACGTCTAGCAAATCTCCGACGCTGTTTGGCTCTGTATCTATGATCGATGATGCGTTAACTATCGGCCCCAAAAGGAGCTCCAAAGTTGTGGGAAGGGCACAAGGGTTTTATGCATCTGCCTCACAAAGTGAAAATGCTTTGATGGTGACCATGAACCTGGCTTTCACCGAAGGGAAGTTTAATGGCAGCAGTCTTACTGTTGTGGGACGAAACCCTGTTGTTGAAGCTGTAAGAGAGATGCCTATAATCGGTGGAAGTGGACTTTTTCGGTTTTCTAGGGGATATGTTCAGGCCAAGACTCTCAGGTTCAATGCATCAACTGGAGATGCCGTTGTTGAATATAATGTGTTTGTGTTCCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

170

Amino Acids

18.44

Weight (kDa)

9.79

Isoelectric Point (pI)

48.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 25 - 168 2.2e-57 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 108
AclWI GGATC 1 cut(s) 62
AdeI CACNNNGTG 1 cut(s) 275
AfiI CCNNNNNNNGG 1 cut(s) 395
AgsI TTSAA 3 cut(s) 371, 457, 485
AjiI CACGTC 1 cut(s) 150
AjnI CCWGG 1 cut(s) 303
AluBI AGCT 3 cut(s) 70, 225, 374
AluI AGCT 3 cut(s) 70, 225, 374
Alw21I GWGCWC 2 cut(s) 145, 227
AlwI GGATC 1 cut(s) 62
Ama87I CYCGRG 1 cut(s) 138
AoxI GGCC 2 cut(s) 211, 438
ApeKI GCWGC 1 cut(s) 333
AspS9I GGNCC 2 cut(s) 110, 212
AsuHPI GGTGA 2 cut(s) 304, 304
AvaI CYCGRG 1 cut(s) 138
AvaII GGWCC 1 cut(s) 110
BaeGI GKGCMC 1 cut(s) 247
BanII GRGCYC 1 cut(s) 227
Bbv12I GWGCWC 2 cut(s) 145, 227
BbvI GCAGC 1 cut(s) 345
BccI CCATC 2 cut(s) 134, 283
BceAI ACGGC 1 cut(s) 461
BciT130I CCWGG 1 cut(s) 305
BfaI CTAG 2 cut(s) 153, 423
BisI GCNGC 1 cut(s) 334
BlpI GCTNAGC 1 cut(s) 71
BlsI GCNGC 1 cut(s) 335
Bme1390I CCNGG 1 cut(s) 305
Bme18I GGWCC 1 cut(s) 110
BmeT110I CYCGRG 1 cut(s) 138
BmgBI CACGTC 1 cut(s) 150
BmgT120I GGNCC 2 cut(s) 110, 212
BmiI GGNNCC 2 cut(s) 112, 214
BmrFI CCNGG 1 cut(s) 305
BmsI GCATC 5 cut(s) 187, 269, 375, 463, 470
BpmI CTGGAG 1 cut(s) 489
Bpu1102I GCTNAGC 1 cut(s) 71
Bsa29I ATCGAT 1 cut(s) 192
BsaXI ACNNNNNCTCC 2 cut(s) 462, 492
Bsc4I CCNNNNNNNGG 1 cut(s) 395
Bse1I ACTGG 1 cut(s) 472
BseBI CCWGG 1 cut(s) 305
BseCI ATCGAT 1 cut(s) 192
BseLI CCNNNNNNNGG 1 cut(s) 395
BseMII CTCAG 2 cut(s) 41, 463
BseNI ACTGG 1 cut(s) 472
BseSI GKGCMC 1 cut(s) 247
BseXI GCAGC 1 cut(s) 345
BshFI GGCC 2 cut(s) 213, 440
BshVI ATCGAT 1 cut(s) 192
BsiHKAI GWGCWC 2 cut(s) 145, 227
BsiHKCI CYCGRG 1 cut(s) 138
BslFI GGGAC 2 cut(s) 96, 366
BslI CCNNNNNNNGG 1 cut(s) 395
BsmFI GGGAC 2 cut(s) 96, 366
BsnI GGCC 2 cut(s) 213, 440
BsoBI CYCGRG 1 cut(s) 138
Bsp1286I GDGCHC 3 cut(s) 145, 227, 247
Bsp143I GATC 2 cut(s) 54, 189
Bsp1720I GCTNAGC 1 cut(s) 71
BspACI CCGC 1 cut(s) 108
BspANI GGCC 2 cut(s) 213, 440
BspCNI CTCAG 2 cut(s) 40, 462
BspDI ATCGAT 1 cut(s) 192
BspLI GGNNCC 2 cut(s) 112, 214
BspPI GGATC 1 cut(s) 62
BsrI ACTGG 1 cut(s) 472
BssMI GATC 2 cut(s) 54, 189
Bst2UI CCWGG 1 cut(s) 305
Bst4CI ACNGT 1 cut(s) 346
BstC8I GCNNGC 1 cut(s) 106
BstDEI CTNAG 3 cut(s) 27, 71, 449
BstEII GGTNACC 1 cut(s) 292
BstKTI GATC 2 cut(s) 57, 192
BstMBI GATC 2 cut(s) 54, 189
BstMWI GCNNNNNNNGC 1 cut(s) 67
BstNI CCWGG 1 cut(s) 305
BstPI GGTNACC 1 cut(s) 292
BstSCI CCNGG 1 cut(s) 303
BstSLI GKGCMC 1 cut(s) 247
BstV1I GCAGC 1 cut(s) 345
BstXI CCANNNNNNTGG 2 cut(s) 235, 304
Bsu15I ATCGAT 1 cut(s) 192
BsuRI GGCC 2 cut(s) 213, 440
BsuTUI ATCGAT 1 cut(s) 192
BtrI CACGTC 1 cut(s) 150
BtsI GCAGTG 1 cut(s) 5
BtsIMutI CAGTG 1 cut(s) 5
Cac8I GCNNGC 1 cut(s) 106
Cfr13I GGNCC 2 cut(s) 110, 212
ClaI ATCGAT 1 cut(s) 192
CseI GACGC 1 cut(s) 175
CviAII CATG 1 cut(s) 298
CviJI RGCY 8 cut(s) 70, 75, 177, 213, 225, 308, 374, 440
CviKI_1 RGCY 8 cut(s) 70, 75, 177, 213, 225, 308, 374, 440
DdeI CTNAG 3 cut(s) 27, 71, 449
DpnI GATC 2 cut(s) 56, 191
DpnII GATC 2 cut(s) 54, 189
DraIII CACNNNGTG 1 cut(s) 275
Ecl136II GAGCTC 1 cut(s) 225
Eco24I GRGCYC 1 cut(s) 227
Eco32I GATATC 1 cut(s) 100
Eco47I GGWCC 1 cut(s) 110
Eco53kI GAGCTC 1 cut(s) 225
Eco88I CYCGRG 1 cut(s) 138
Eco91I GGTNACC 1 cut(s) 292
EcoICRI GAGCTC 1 cut(s) 225
EcoO65I GGTNACC 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 303
EcoRV GATATC 1 cut(s) 100
EcoT22I ATGCAT 2 cut(s) 262, 463
EcoT38I GRGCYC 1 cut(s) 227
FaeI CATG 1 cut(s) 301
FaiI YATR 6 cut(s) 188, 258, 299, 392, 432, 489
FaqI GGGAC 2 cut(s) 96, 366
FatI CATG 1 cut(s) 297
Fnu4HI GCNGC 1 cut(s) 334
FriOI GRGCYC 1 cut(s) 227
Fsp4HI GCNGC 1 cut(s) 334
FspBI CTAG 2 cut(s) 153, 423
GluI GCNGC 1 cut(s) 334
GsuI CTGGAG 1 cut(s) 489
HaeIII GGCC 2 cut(s) 213, 440
HgaI GACGC 1 cut(s) 175
Hin1II CATG 1 cut(s) 301
HincII GTYRAC 1 cut(s) 204
HindII GTYRAC 1 cut(s) 204
HinfI GANTC 1 cut(s) 445
HpaI GTTAAC 1 cut(s) 204
HphI GGTGA 2 cut(s) 304, 304
Hpy166II GTNNAC 2 cut(s) 204, 407
Hpy188I TCNGA 2 cut(s) 30, 165
Hpy8I GTNNAC 2 cut(s) 204, 407
Hpy99I CGWCG 1 cut(s) 169
HpyAV CCTTC 2 cut(s) 234, 311
HpyCH4III ACNGT 1 cut(s) 346
HpyCH4IV ACGT 1 cut(s) 149
HpyCH4V TGCA 3 cut(s) 10, 260, 461
HpyF10VI GCNNNNNNNGC 1 cut(s) 67
HpyF3I CTNAG 3 cut(s) 27, 71, 449
HpySE526I ACGT 1 cut(s) 149
Hsp92II CATG 1 cut(s) 301
KspAI GTTAAC 1 cut(s) 204
Kzo9I GATC 2 cut(s) 54, 189
LmnI GCTCC 2 cut(s) 222, 230
LpnPI CCDG 8 cut(s) 115, 118, 290, 317, 375, 422, 436, 453
Lsp1109I GCAGC 1 cut(s) 345
LweI GCATC 5 cut(s) 187, 269, 375, 463, 470
MaeI CTAG 2 cut(s) 153, 423
MaeII ACGT 1 cut(s) 149
MaeIII GTNAC 1 cut(s) 292
MalI GATC 2 cut(s) 56, 191
MboI GATC 2 cut(s) 54, 189
MboII GAAGA 1 cut(s) 15
MhlI GDGCHC 3 cut(s) 145, 227, 247
MlyI GAGTC 1 cut(s) 439
MmeI TCCRAC 1 cut(s) 188
MnlI CCTC 4 cut(s) 27, 48, 89, 277
Mph1103I ATGCAT 2 cut(s) 262, 463
MseI TTAA 2 cut(s) 203, 327
MspA1I CMGCKG 1 cut(s) 108
MspR9I CCNGG 1 cut(s) 305
MvaI CCWGG 1 cut(s) 305
MwoI GCNNNNNNNGC 1 cut(s) 67
NdeII GATC 2 cut(s) 54, 189
NlaIII CATG 1 cut(s) 301
NlaIV GGNNCC 2 cut(s) 112, 214
NmuCI GTSAC 1 cut(s) 292
NsiI ATGCAT 2 cut(s) 262, 463
PkrI GCNGC 1 cut(s) 335
PleI GAGTC 1 cut(s) 439
PpsI GAGTC 1 cut(s) 439
Psp124BI GAGCTC 1 cut(s) 227
Psp6I CCWGG 1 cut(s) 303
PspEI GGTNACC 1 cut(s) 292
PspGI CCWGG 1 cut(s) 303
PspN4I GGNNCC 2 cut(s) 112, 214
PspPI GGNCC 2 cut(s) 110, 212
SacI GAGCTC 1 cut(s) 227
SaqAI TTAA 2 cut(s) 203, 327
SatI GCNGC 1 cut(s) 334
Sau3AI GATC 2 cut(s) 54, 189
Sau96I GGNCC 2 cut(s) 110, 212
SchI GAGTC 1 cut(s) 439
ScrFI CCNGG 1 cut(s) 305
SduI GDGCHC 3 cut(s) 145, 227, 247
SetI ASST 8 cut(s) 40, 72, 81, 152, 227, 306, 376, 455
SfaNI GCATC 5 cut(s) 187, 269, 375, 463, 470
SinI GGWCC 1 cut(s) 110
SsiI CCGC 1 cut(s) 108
SspMI CTAG 2 cut(s) 153, 423
SstI GAGCTC 1 cut(s) 227
StyD4I CCNGG 1 cut(s) 303
TaaI ACNGT 1 cut(s) 346
TaiI ACGT 1 cut(s) 152
TaqI TCGA 1 cut(s) 192
Tru1I TTAA 2 cut(s) 203, 327
Tru9I TTAA 2 cut(s) 203, 327
TscAI CASTG 1 cut(s) 12
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 1 cut(s) 333
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 1 cut(s) 314
TspRI CASTG 1 cut(s) 12
VpaK11BI GGWCC 1 cut(s) 110
XcmI CCANNNNNNNNNTGG 1 cut(s) 126
XspI CTAG 2 cut(s) 153, 423
Zsp2I ATGCAT 2 cut(s) 262, 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.