Rw3G019880

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0003503
Physical Location & Seq
Forward (+)
23038384 .. 23039238
855 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G019880.1

Sequence Viewer

Length: 585 bp
ATGGCTAAGACCCTCCAAAACTTCAGTTCCCTCTGCACCATTCCCTTGACTCTTCTCCTCCTCTTAACCCTTGTCGGTGCCAGCGAGCACCGCTTCTCAAGACCCTTGTCCCCAGCCAAACTTGGCCTAAAGAAAGAGAAACTGAGCCATCTCCGGTTCTACTTCCACGACATAGTCAGCGGCCACAGCCCAACAGCCGTTAGGGTCGCTGAGGCTCCCACGACCAAAAACTCCTCCACGGCTTTCGGTGCAGTGGTGATGATGGATGACCCCTTGACTGCCGGACCGGAGCTGGGAACCAAGCTAGTCGGCAAGGCACAAGGCATTTATGCTTCGGCCTCGCAAACCGAGTTAGGGCTTTTGATGGTGCTGAACTTTGCTTTTGTGGAAGGGAAGTATAATGGGAGTAATCTGAGCGTGTTGGGAAGGAACGCGGTGTTCTCCGCCGTGAGGGAGATGCCAATAGTTGGCGGGAGCGGCCTTTTCCGGTTTGCTAGGGGGTATGCTCGTGCTAGGACTCACAAGATTGATTTGAACACTGGGGATGCTGTGGTGGAGTATAATGTCTACGTTCTCCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

194

Amino Acids

20.96

Weight (kDa)

9.64

Isoelectric Point (pI)

28.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 49 - 191 1.8e-58 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 77
AccB7I CCANNNNNTGG 1 cut(s) 467
AccBSI CCGCTC 1 cut(s) 477
AccI GTMKAC 1 cut(s) 567
AccII CGCG 1 cut(s) 434
AciI CCGC 6 cut(s) 91, 180, 434, 444, 471, 477
AcoI YGGCCR 1 cut(s) 181
AcuI CTGAAG 1 cut(s) 7
AfiI CCNNNNNNNGG 4 cut(s) 293, 354, 450, 467
AgsI TTSAA 1 cut(s) 535
AluBI AGCT 2 cut(s) 292, 304
AluI AGCT 2 cut(s) 292, 304
Alw21I GWGCWC 1 cut(s) 90
AoxI GGCC 4 cut(s) 124, 181, 336, 478
AspS9I GGNCC 1 cut(s) 284
AsuHPI GGTGA 1 cut(s) 268
AvaII GGWCC 1 cut(s) 284
BanI GGYRCC 1 cut(s) 77
BauI CACGAG 1 cut(s) 507
Bbv12I GWGCWC 1 cut(s) 90
BbvCI CCTCAGC 1 cut(s) 210
BccI CCATC 3 cut(s) 156, 256, 358
BceAI ACGGC 3 cut(s) 182, 255, 431
BfaI CTAG 3 cut(s) 305, 495, 513
BisI GCNGC 2 cut(s) 181, 478
BlsI GCNGC 2 cut(s) 182, 479
Bme18I GGWCC 1 cut(s) 284
BmgT120I GGNCC 1 cut(s) 284
BmiI GGNNCC 3 cut(s) 79, 216, 298
BmrI ACTGGG 1 cut(s) 549
BmsI GCATC 2 cut(s) 447, 535
BmuI ACTGGG 1 cut(s) 549
BoxI GACNNNNGTC 1 cut(s) 106
Bpu10I CCTNAGC 1 cut(s) 210
BpuEI CTTGAG 1 cut(s) 82
BsaJI CCNNGG 1 cut(s) 237
BsaWI WCCGGW 3 cut(s) 153, 286, 486
Bsc4I CCNNNNNNNGG 4 cut(s) 293, 354, 450, 467
Bse1I ACTGG 1 cut(s) 544
BseDI CCNNGG 1 cut(s) 237
BseGI GGATG 2 cut(s) 271, 550
BseLI CCNNNNNNNGG 4 cut(s) 293, 354, 450, 467
BseMII CTCAG 3 cut(s) 134, 201, 404
BseNI ACTGG 1 cut(s) 544
BseRI GAGGAG 3 cut(s) 47, 50, 223
BseYI CCCAGC 2 cut(s) 112, 292
BsgI GTGCAG 2 cut(s) 19, 270
Bsh1236I CGCG 1 cut(s) 434
BshFI GGCC 4 cut(s) 126, 183, 338, 480
BshNI GGYRCC 1 cut(s) 77
BsiHKAI GWGCWC 1 cut(s) 90
BsiSI CCGG 4 cut(s) 154, 282, 287, 487
BslFI GGGAC 1 cut(s) 94
BslI CCNNNNNNNGG 4 cut(s) 293, 354, 450, 467
BsmFI GGGAC 1 cut(s) 94
BsnI GGCC 4 cut(s) 126, 183, 338, 480
Bsp1286I GDGCHC 1 cut(s) 90
BspACI CCGC 6 cut(s) 91, 180, 434, 444, 471, 477
BspANI GGCC 4 cut(s) 126, 183, 338, 480
BspCNI CTCAG 3 cut(s) 135, 202, 405
BspFNI CGCG 1 cut(s) 434
BspLI GGNNCC 3 cut(s) 79, 216, 298
BspT107I GGYRCC 1 cut(s) 77
BsrBI CCGCTC 1 cut(s) 477
BsrI ACTGG 1 cut(s) 544
BssECI CCNNGG 1 cut(s) 237
BssSI CACGAG 1 cut(s) 507
Bst2BI CACGAG 1 cut(s) 507
Bst6I CTCTTC 1 cut(s) 57
BstC8I GCNNGC 2 cut(s) 82, 86
BstDEI CTNAG 4 cut(s) 6, 143, 210, 413
BstDSI CCRYGG 1 cut(s) 237
BstF5I GGATG 2 cut(s) 271, 550
BstFNI CGCG 1 cut(s) 434
BstMWI GCNNNNNNNGC 4 cut(s) 90, 186, 248, 477
BstPAI GACNNNNGTC 1 cut(s) 106
BstUI CGCG 1 cut(s) 434
BsuRI GGCC 4 cut(s) 126, 183, 338, 480
BtgI CCRYGG 1 cut(s) 237
BtsCI GGATG 2 cut(s) 271, 550
BtsI GCAGTG 1 cut(s) 258
BtsIMutI CAGTG 2 cut(s) 258, 537
Cac8I GCNNGC 2 cut(s) 82, 86
Cfr13I GGNCC 1 cut(s) 284
CpoI CGGWCCG 1 cut(s) 284
CspI CGGWCCG 1 cut(s) 284
DdeI CTNAG 4 cut(s) 6, 143, 210, 413
EaeI YGGCCR 1 cut(s) 181
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
EciI GGCGGA 1 cut(s) 433
Eco47I GGWCC 1 cut(s) 284
Eco57I CTGAAG 1 cut(s) 7
FaiI YATR 5 cut(s) 173, 330, 399, 504, 561
FaqI GGGAC 1 cut(s) 94
FauI CCCGC 1 cut(s) 464
FblI GTMKAC 1 cut(s) 567
Fnu4HI GCNGC 2 cut(s) 181, 478
FokI GGATG 2 cut(s) 278, 557
Fsp4HI GCNGC 2 cut(s) 181, 478
FspBI CTAG 3 cut(s) 305, 495, 513
GluI GCNGC 2 cut(s) 181, 478
GsaI CCCAGC 2 cut(s) 116, 296
HaeIII GGCC 4 cut(s) 126, 183, 338, 480
HapII CCGG 4 cut(s) 154, 282, 287, 487
HinfI GANTC 2 cut(s) 49, 517
HpaII CCGG 4 cut(s) 154, 282, 287, 487
HphI GGTGA 1 cut(s) 268
Hpy166II GTNNAC 1 cut(s) 568
Hpy188I TCNGA 1 cut(s) 414
Hpy188III TCNNGA 1 cut(s) 99
Hpy8I GTNNAC 1 cut(s) 568
HpyAV CCTTC 2 cut(s) 383, 420
HpyCH4IV ACGT 1 cut(s) 570
HpyCH4V TGCA 2 cut(s) 36, 251
HpyF10VI GCNNNNNNNGC 4 cut(s) 90, 186, 248, 477
HpyF3I CTNAG 4 cut(s) 6, 143, 210, 413
HpySE526I ACGT 1 cut(s) 570
LmnI GCTCC 3 cut(s) 220, 289, 474
LpnPI CCDG 8 cut(s) 94, 126, 167, 278, 295, 300, 500, 525
LweI GCATC 2 cut(s) 447, 535
MaeI CTAG 3 cut(s) 305, 495, 513
MaeII ACGT 1 cut(s) 570
MbiI CCGCTC 1 cut(s) 477
MboII GAAGA 1 cut(s) 44
MhlI GDGCHC 1 cut(s) 90
MlyI GAGTC 2 cut(s) 43, 511
MnlI CCTC 8 cut(s) 23, 41, 68, 71, 205, 244, 349, 444
MseI TTAA 1 cut(s) 65
MspA1I CMGCKG 1 cut(s) 180
MspI CCGG 4 cut(s) 154, 282, 287, 487
MvnI CGCG 1 cut(s) 434
MwoI GCNNNNNNNGC 4 cut(s) 90, 186, 248, 477
NlaIV GGNNCC 3 cut(s) 79, 216, 298
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PflFI GACNNNGTC 1 cut(s) 173
PflMI CCANNNNNTGG 1 cut(s) 467
PkrI GCNGC 2 cut(s) 182, 479
PleI GAGTC 2 cut(s) 43, 511
PpsI GAGTC 2 cut(s) 43, 511
PshAI GACNNNNGTC 1 cut(s) 106
PspFI CCCAGC 2 cut(s) 112, 292
PspN4I GGNNCC 3 cut(s) 79, 216, 298
PspPI GGNCC 1 cut(s) 284
PsyI GACNNNGTC 1 cut(s) 173
Rsr2I CGGWCCG 1 cut(s) 284
RsrII CGGWCCG 1 cut(s) 284
SaqAI TTAA 1 cut(s) 65
SatI GCNGC 2 cut(s) 181, 478
Sau96I GGNCC 1 cut(s) 284
SchI GAGTC 2 cut(s) 43, 511
SduI GDGCHC 1 cut(s) 90
SetI ASST 3 cut(s) 294, 306, 573
SfaNI GCATC 2 cut(s) 447, 535
SinI GGWCC 1 cut(s) 284
SmlI CTYRAG 1 cut(s) 97
SmoI CTYRAG 1 cut(s) 97
SsiI CCGC 6 cut(s) 91, 180, 434, 444, 471, 477
SspMI CTAG 3 cut(s) 305, 495, 513
TaiI ACGT 1 cut(s) 573
TauI GCSGC 2 cut(s) 183, 480
Tru1I TTAA 1 cut(s) 65
Tru9I TTAA 1 cut(s) 65
TscAI CASTG 2 cut(s) 258, 544
TspRI CASTG 2 cut(s) 258, 544
Tth111I GACNNNGTC 1 cut(s) 173
Van91I CCANNNNNTGG 1 cut(s) 467
VpaK11BI GGWCC 1 cut(s) 284
XmiI GTMKAC 1 cut(s) 567
XspI CTAG 3 cut(s) 305, 495, 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.