RLG00000023613

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0003503
Physical Location & Seq
Reverse (-)
26022398 .. 26024817
2420 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023613

Sequence Viewer

Length: 708 bp
ATGGTTTTCTGGGATTTTTCTGGCAATTGTTCAGGAGGTTCTGGGCTTGTATCTGGCGTTTTGGAGCTTGATAAATGCCTGGTCGACAGCGAGGTGGCGGAGCTTCTTGGAGAGGTGGAGGGCGGGGAGTGTGGTGGAGGAGCTCGGAGTTCCCTCTGCACCATTCCCTTGACTCTTCTCCTCCTCTTAACCCTCGTCGGTGCCAGCGAGCACGGCTTCTCAAGACCCTTGTCCCGAGCCAAACTTGGCCTAAAGAAAGAGAAACTGAGCCATCTCCGGTTCTACTTCCACGACATAGTCAGCGGCCACAGCCCAACAGCCGTTAGGGTCGCTGAGGCTCCCACGACCAAAAACTCCTCCACGGCTTTCGGTGCAGTGGTGATGATGGATGACCCCTTGACTGCCGGGCCGGAGCTGGGAACCAAGCTAGTCGGCAAGGCGCAAGGCATTTATGCTTCGGCCTCGCAAACCGAGTTAGGGTTTTTGATGGTGCTGAACTTTGCGTTTGTGGAAGGGAAGTATAATGGGAGTAATCTGAGCGTGTTGGGAAGGAACGCGGTGTTCTCCGCCGTGAGGGAGATGCCAATAGTTGGCGGGAGCGGCCTTTTCCGGTTTGCTAGGGGGTATGCCCGTGCTAGGACTCACAAGGTTGATTTGAACACTGGGGATGCTGTGGTGGAGTATAATGTCTACGTTCTCCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

236

Amino Acids

24.93

Weight (kDa)

6.59

Isoelectric Point (pI)

26.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 90 - 232 4.1e-58 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 200
AccB7I CCANNNNNTGG 1 cut(s) 590
AccBSI CCGCTC 1 cut(s) 600
AccI GTMKAC 2 cut(s) 84, 690
AccII CGCG 1 cut(s) 557
AciI CCGC 7 cut(s) 98, 123, 303, 557, 567, 594, 600
AcoI YGGCCR 1 cut(s) 304
AfiI CCNNNNNNNGG 5 cut(s) 416, 477, 573, 590, 636
AgsI TTSAA 1 cut(s) 658
AjnI CCWGG 1 cut(s) 78
AluBI AGCT 5 cut(s) 67, 103, 143, 415, 427
AluI AGCT 5 cut(s) 67, 103, 143, 415, 427
Alw21I GWGCWC 2 cut(s) 145, 213
Ama87I CYCGRG 1 cut(s) 234
AoxI GGCC 5 cut(s) 247, 304, 407, 459, 601
AspLEI GCGC 1 cut(s) 442
AspS9I GGNCC 1 cut(s) 407
AsuC2I CCSGG 1 cut(s) 406
AsuHPI GGTGA 1 cut(s) 391
AvaI CYCGRG 1 cut(s) 234
BanI GGYRCC 1 cut(s) 200
BanII GRGCYC 1 cut(s) 145
Bbv12I GWGCWC 2 cut(s) 145, 213
BbvCI CCTCAGC 1 cut(s) 333
BccI CCATC 3 cut(s) 279, 379, 481
BceAI ACGGC 4 cut(s) 229, 305, 378, 554
BciT130I CCWGG 1 cut(s) 80
BcnI CCSGG 1 cut(s) 406
BfaI CTAG 3 cut(s) 428, 618, 636
BisI GCNGC 2 cut(s) 304, 601
BlsI GCNGC 2 cut(s) 305, 602
Bme1390I CCNGG 2 cut(s) 80, 406
BmeT110I CYCGRG 1 cut(s) 234
BmgT120I GGNCC 1 cut(s) 407
BmiI GGNNCC 3 cut(s) 202, 339, 421
BmrFI CCNGG 2 cut(s) 80, 406
BmrI ACTGGG 1 cut(s) 672
BmsI GCATC 2 cut(s) 570, 658
BmuI ACTGGG 1 cut(s) 672
BoxI GACNNNNGTC 1 cut(s) 229
Bpu10I CCTNAGC 1 cut(s) 333
BpuEI CTTGAG 1 cut(s) 205
BpuMI CCSGG 1 cut(s) 406
BsaJI CCNNGG 1 cut(s) 360
BsaWI WCCGGW 2 cut(s) 276, 609
Bsc4I CCNNNNNNNGG 5 cut(s) 416, 477, 573, 590, 636
Bse1I ACTGG 1 cut(s) 667
BseBI CCWGG 1 cut(s) 80
BseDI CCNNGG 1 cut(s) 360
BseGI GGATG 2 cut(s) 394, 673
BseLI CCNNNNNNNGG 5 cut(s) 416, 477, 573, 590, 636
BseMII CTCAG 3 cut(s) 257, 324, 527
BseNI ACTGG 1 cut(s) 667
BseRI GAGGAG 4 cut(s) 153, 170, 173, 346
BseYI CCCAGC 1 cut(s) 415
BsgI GTGCAG 2 cut(s) 142, 393
Bsh1236I CGCG 1 cut(s) 557
BshFI GGCC 5 cut(s) 249, 306, 409, 461, 603
BshNI GGYRCC 1 cut(s) 200
BsiHKAI GWGCWC 2 cut(s) 145, 213
BsiHKCI CYCGRG 1 cut(s) 234
BsiSI CCGG 4 cut(s) 277, 405, 410, 610
BslFI GGGAC 1 cut(s) 217
BslI CCNNNNNNNGG 5 cut(s) 416, 477, 573, 590, 636
BsmFI GGGAC 1 cut(s) 217
BsnI GGCC 5 cut(s) 249, 306, 409, 461, 603
BsoBI CYCGRG 1 cut(s) 234
Bsp1286I GDGCHC 2 cut(s) 145, 213
BspACI CCGC 7 cut(s) 98, 123, 303, 557, 567, 594, 600
BspANI GGCC 5 cut(s) 249, 306, 409, 461, 603
BspCNI CTCAG 3 cut(s) 258, 325, 528
BspFNI CGCG 1 cut(s) 557
BspLI GGNNCC 3 cut(s) 202, 339, 421
BspT107I GGYRCC 1 cut(s) 200
BsrBI CCGCTC 1 cut(s) 600
BsrI ACTGG 1 cut(s) 667
BssECI CCNNGG 1 cut(s) 360
Bst2UI CCWGG 1 cut(s) 80
Bst6I CTCTTC 1 cut(s) 180
BstC8I GCNNGC 2 cut(s) 205, 209
BstDEI CTNAG 3 cut(s) 266, 333, 536
BstDSI CCRYGG 1 cut(s) 360
BstF5I GGATG 2 cut(s) 394, 673
BstFNI CGCG 1 cut(s) 557
BstHHI GCGC 1 cut(s) 442
BstMWI GCNNNNNNNGC 4 cut(s) 213, 309, 371, 600
BstNI CCWGG 1 cut(s) 80
BstPAI GACNNNNGTC 1 cut(s) 229
BstSCI CCNGG 2 cut(s) 78, 404
BstUI CGCG 1 cut(s) 557
BsuRI GGCC 5 cut(s) 249, 306, 409, 461, 603
BtgI CCRYGG 1 cut(s) 360
BtsCI GGATG 2 cut(s) 394, 673
BtsI GCAGTG 1 cut(s) 381
BtsIMutI CAGTG 2 cut(s) 381, 660
Cac8I GCNNGC 2 cut(s) 205, 209
CfoI GCGC 1 cut(s) 442
Cfr13I GGNCC 1 cut(s) 407
DdeI CTNAG 3 cut(s) 266, 333, 536
EaeI YGGCCR 1 cut(s) 304
Eam1104I CTCTTC 1 cut(s) 180
EarI CTCTTC 1 cut(s) 180
EciI GGCGGA 2 cut(s) 113, 556
Ecl136II GAGCTC 1 cut(s) 143
Eco24I GRGCYC 1 cut(s) 145
Eco53kI GAGCTC 1 cut(s) 143
Eco88I CYCGRG 1 cut(s) 234
EcoICRI GAGCTC 1 cut(s) 143
EcoRII CCWGG 1 cut(s) 78
EcoT38I GRGCYC 1 cut(s) 145
FaiI YATR 5 cut(s) 296, 453, 522, 627, 684
FaqI GGGAC 1 cut(s) 217
FauI CCCGC 2 cut(s) 116, 587
FblI GTMKAC 2 cut(s) 84, 690
Fnu4HI GCNGC 2 cut(s) 304, 601
FokI GGATG 2 cut(s) 401, 680
FriOI GRGCYC 1 cut(s) 145
Fsp4HI GCNGC 2 cut(s) 304, 601
FspBI CTAG 3 cut(s) 428, 618, 636
GlaI GCGC 1 cut(s) 441
GluI GCNGC 2 cut(s) 304, 601
GsaI CCCAGC 1 cut(s) 419
HaeIII GGCC 5 cut(s) 249, 306, 409, 461, 603
HapII CCGG 4 cut(s) 277, 405, 410, 610
HhaI GCGC 1 cut(s) 442
Hin6I GCGC 1 cut(s) 440
HinP1I GCGC 1 cut(s) 440
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HinfI GANTC 2 cut(s) 172, 640
HpaII CCGG 4 cut(s) 277, 405, 410, 610
HphI GGTGA 1 cut(s) 391
Hpy166II GTNNAC 2 cut(s) 85, 691
Hpy188I TCNGA 2 cut(s) 147, 537
Hpy188III TCNNGA 3 cut(s) 33, 222, 234
Hpy8I GTNNAC 2 cut(s) 85, 691
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 2 cut(s) 506, 543
HpyCH4IV ACGT 1 cut(s) 693
HpyCH4V TGCA 2 cut(s) 159, 374
HpyF10VI GCNNNNNNNGC 4 cut(s) 213, 309, 371, 600
HpyF3I CTNAG 3 cut(s) 266, 333, 536
HpySE526I ACGT 1 cut(s) 693
HspAI GCGC 1 cut(s) 440
LmnI GCTCC 6 cut(s) 64, 100, 140, 343, 412, 597
LweI GCATC 2 cut(s) 570, 658
MaeI CTAG 3 cut(s) 428, 618, 636
MaeII ACGT 1 cut(s) 693
MbiI CCGCTC 1 cut(s) 600
MboII GAAGA 1 cut(s) 167
MfeI CAATTG 1 cut(s) 25
MhlI GDGCHC 2 cut(s) 145, 213
MluCI AATT 1 cut(s) 25
MlyI GAGTC 2 cut(s) 166, 634
MseI TTAA 1 cut(s) 188
MspA1I CMGCKG 1 cut(s) 303
MspI CCGG 4 cut(s) 277, 405, 410, 610
MspR9I CCNGG 2 cut(s) 80, 406
MunI CAATTG 1 cut(s) 25
MvaI CCWGG 1 cut(s) 80
MvnI CGCG 1 cut(s) 557
MwoI GCNNNNNNNGC 4 cut(s) 213, 309, 371, 600
NciI CCSGG 1 cut(s) 406
NlaIV GGNNCC 3 cut(s) 202, 339, 421
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PflFI GACNNNGTC 1 cut(s) 296
PflMI CCANNNNNTGG 1 cut(s) 590
PkrI GCNGC 2 cut(s) 305, 602
PleI GAGTC 2 cut(s) 166, 634
PpsI GAGTC 2 cut(s) 166, 634
PshAI GACNNNNGTC 1 cut(s) 229
Psp124BI GAGCTC 1 cut(s) 145
Psp6I CCWGG 1 cut(s) 78
PspFI CCCAGC 1 cut(s) 415
PspGI CCWGG 1 cut(s) 78
PspN4I GGNNCC 3 cut(s) 202, 339, 421
PspPI GGNCC 1 cut(s) 407
PsyI GACNNNGTC 1 cut(s) 296
SacI GAGCTC 1 cut(s) 145
SalI GTCGAC 1 cut(s) 83
SaqAI TTAA 1 cut(s) 188
SatI GCNGC 2 cut(s) 304, 601
Sau96I GGNCC 1 cut(s) 407
SchI GAGTC 2 cut(s) 166, 634
ScrFI CCNGG 2 cut(s) 80, 406
SduI GDGCHC 2 cut(s) 145, 213
SfaNI GCATC 2 cut(s) 570, 658
SmlI CTYRAG 1 cut(s) 220
SmoI CTYRAG 1 cut(s) 220
Sse9I AATT 1 cut(s) 25
SsiI CCGC 7 cut(s) 98, 123, 303, 557, 567, 594, 600
SspMI CTAG 3 cut(s) 428, 618, 636
SstI GAGCTC 1 cut(s) 145
StyD4I CCNGG 2 cut(s) 78, 404
TaiI ACGT 1 cut(s) 696
TaqI TCGA 1 cut(s) 84
TasI AATT 1 cut(s) 25
TauI GCSGC 2 cut(s) 306, 603
Tru1I TTAA 1 cut(s) 188
Tru9I TTAA 1 cut(s) 188
TscAI CASTG 2 cut(s) 381, 667
TspRI CASTG 2 cut(s) 381, 667
Tth111I GACNNNGTC 1 cut(s) 296
Van91I CCANNNNNTGG 1 cut(s) 590
XmiI GTMKAC 2 cut(s) 84, 690
XspI CTAG 3 cut(s) 428, 618, 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.