FvH4_6g21711

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003503
Physical Location & Seq
Forward (+)
15247672 .. 15248396
725 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g21711.t1

Sequence Viewer

Length: 585 bp
ATGGCCAAGACCCTCCGAAACTTCGTTTCCCACTACACCATTCTCTTGACTCTTCTCTTCCTCTTAACCCTTGTCGGTGCCGGCGACCGTCACTTCTCGAGGCCCTTGTCTCGAGCCAAACACGGTCTAAAGAAAGAGAAACTGAGCCATGTACATTTCTACTTCCACGACATAGTCAGCGGTCGCAGCCCGACAGCCATTAGGGTCGCCGAGGCTCCCACGACCCAAAACTCCTCCACAGCTTTCGGTGCGGTGGTGATGATTGACGACCCCTTGACTGCCGGACCGGAGCTGGGATCCAAGCTAGTCGGAAAGGCACAAGGCATTTATGCTTCTGCCTCGCAAACCGAGCTAGGGTTTTTGATGGTGCTGAACTTTGCTTTTATGGAAGGCAAGTATAATGGGAGTTATCTGAGCGTGTTGGGAAGGAACACAGTGTTCTCCGCCGTGAGGGAGATGCCGATAGTCGGCGGGAGCGGCCTTTTCCGGTTTGCTAGGGGTTATGCTCGTGCTAAGACTCACAAGGTTGATTTTAACACTGGGGATGCTGTTGTGGAGTATAATGTATACGTTCTCCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

195

Amino Acids

21.28

Weight (kDa)

9.76

Isoelectric Point (pI)

24.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 49 - 191 7.8e-59 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 77
AccBSI CCGCTC 1 cut(s) 477
AccI GTMKAC 1 cut(s) 567
AciI CCGC 5 cut(s) 180, 251, 444, 471, 477
AclWI GGATC 2 cut(s) 291, 304
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 1 cut(s) 153
AfiI CCNNNNNNNGG 4 cut(s) 293, 354, 450, 467
AluBI AGCT 4 cut(s) 242, 292, 304, 352
AluI AGCT 4 cut(s) 242, 292, 304, 352
Alw26I GTCTC 1 cut(s) 114
AlwI GGATC 2 cut(s) 291, 304
Ama87I CYCGRG 2 cut(s) 97, 111
AoxI GGCC 3 cut(s) 3, 101, 478
ApeKI GCWGC 1 cut(s) 186
AspS9I GGNCC 2 cut(s) 102, 284
AsuHPI GGTGA 1 cut(s) 268
AvaI CYCGRG 2 cut(s) 97, 111
AvaII GGWCC 1 cut(s) 284
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 296
BanI GGYRCC 1 cut(s) 77
BauI CACGAG 1 cut(s) 507
BbvI GCAGC 1 cut(s) 198
BccI CCATC 1 cut(s) 358
BceAI ACGGC 1 cut(s) 431
BcoDI GTCTC 1 cut(s) 114
BfaI CTAG 3 cut(s) 305, 353, 495
BisI GCNGC 2 cut(s) 187, 478
BlsI GCNGC 2 cut(s) 188, 479
Bme18I GGWCC 1 cut(s) 284
BmeT110I CYCGRG 2 cut(s) 97, 111
BmgT120I GGNCC 2 cut(s) 102, 284
BmiI GGNNCC 3 cut(s) 79, 216, 298
BmrI ACTGGG 1 cut(s) 549
BmsI GCATC 2 cut(s) 447, 535
BmuI ACTGGG 1 cut(s) 549
BsaJI CCNNGG 1 cut(s) 210
BsaWI WCCGGW 2 cut(s) 286, 486
Bsc4I CCNNNNNNNGG 4 cut(s) 293, 354, 450, 467
Bse118I RCCGGY 1 cut(s) 80
Bse1I ACTGG 1 cut(s) 544
BseDI CCNNGG 1 cut(s) 210
BseGI GGATG 1 cut(s) 550
BseLI CCNNNNNNNGG 4 cut(s) 293, 354, 450, 467
BseMII CTCAG 2 cut(s) 134, 404
BseNI ACTGG 1 cut(s) 544
BseRI GAGGAG 1 cut(s) 223
BseXI GCAGC 1 cut(s) 198
BseYI CCCAGC 1 cut(s) 292
Bsh1285I CGRYCG 2 cut(s) 88, 184
BshFI GGCC 3 cut(s) 5, 103, 480
BshNI GGYRCC 1 cut(s) 77
BsiEI CGRYCG 2 cut(s) 88, 184
BsiHKCI CYCGRG 2 cut(s) 97, 111
BsiSI CCGG 4 cut(s) 81, 282, 287, 487
BslI CCNNNNNNNGG 4 cut(s) 293, 354, 450, 467
BsmAI GTCTC 1 cut(s) 114
BsnI GGCC 3 cut(s) 5, 103, 480
BsoBI CYCGRG 2 cut(s) 97, 111
Bsp1407I TGTACA 1 cut(s) 151
Bsp143I GATC 1 cut(s) 296
BspACI CCGC 5 cut(s) 180, 251, 444, 471, 477
BspANI GGCC 3 cut(s) 5, 103, 480
BspCNI CTCAG 2 cut(s) 135, 405
BspLI GGNNCC 3 cut(s) 79, 216, 298
BspPI GGATC 2 cut(s) 291, 304
BspT107I GGYRCC 1 cut(s) 77
BsrBI CCGCTC 1 cut(s) 477
BsrFI RCCGGY 1 cut(s) 80
BsrGI TGTACA 1 cut(s) 151
BsrI ACTGG 1 cut(s) 544
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 1 cut(s) 210
BssMI GATC 1 cut(s) 296
BssNAI GTATAC 1 cut(s) 568
BssSI CACGAG 1 cut(s) 507
Bst1107I GTATAC 1 cut(s) 568
Bst2BI CACGAG 1 cut(s) 507
Bst4CI ACNGT 3 cut(s) 89, 125, 436
Bst6I CTCTTC 2 cut(s) 57, 62
BstAUI TGTACA 1 cut(s) 151
BstC8I GCNNGC 1 cut(s) 82
BstDEI CTNAG 3 cut(s) 143, 413, 513
BstF5I GGATG 1 cut(s) 550
BstKTI GATC 1 cut(s) 299
BstMAI GTCTC 1 cut(s) 114
BstMBI GATC 1 cut(s) 296
BstMCI CGRYCG 2 cut(s) 88, 184
BstMWI GCNNNNNNNGC 4 cut(s) 186, 248, 349, 477
BstV1I GCAGC 1 cut(s) 198
BstX2I RGATCY 1 cut(s) 296
BstYI RGATCY 1 cut(s) 296
BstZ17I GTATAC 1 cut(s) 568
BsuRI GGCC 3 cut(s) 5, 103, 480
BtsCI GGATG 1 cut(s) 550
BtsIMutI CAGTG 2 cut(s) 441, 537
Cac8I GCNNGC 1 cut(s) 82
Cfr10I RCCGGY 1 cut(s) 80
Cfr13I GGNCC 2 cut(s) 102, 284
CpoI CGGWCCG 1 cut(s) 284
Csp6I GTAC 1 cut(s) 152
CspI CGGWCCG 1 cut(s) 284
CviAII CATG 1 cut(s) 149
CviQI GTAC 1 cut(s) 152
DdeI CTNAG 3 cut(s) 143, 413, 513
DpnI GATC 1 cut(s) 298
DpnII GATC 1 cut(s) 296
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 57, 62
EarI CTCTTC 2 cut(s) 57, 62
EciI GGCGGA 1 cut(s) 433
Eco47I GGWCC 1 cut(s) 284
Eco88I CYCGRG 2 cut(s) 97, 111
EcoO109I RGGNCCY 1 cut(s) 102
FaeI CATG 1 cut(s) 152
FaiI YATR 8 cut(s) 150, 173, 330, 386, 399, 504, 561, 568
FatI CATG 1 cut(s) 148
FauI CCCGC 1 cut(s) 464
FblI GTMKAC 1 cut(s) 567
Fnu4HI GCNGC 2 cut(s) 187, 478
FokI GGATG 1 cut(s) 557
Fsp4HI GCNGC 2 cut(s) 187, 478
FspBI CTAG 3 cut(s) 305, 353, 495
GluI GCNGC 2 cut(s) 187, 478
GsaI CCCAGC 1 cut(s) 296
HaeIII GGCC 3 cut(s) 5, 103, 480
HapII CCGG 4 cut(s) 81, 282, 287, 487
Hin1II CATG 1 cut(s) 152
HinfI GANTC 2 cut(s) 49, 517
HpaII CCGG 4 cut(s) 81, 282, 287, 487
HphI GGTGA 1 cut(s) 268
Hpy166II GTNNAC 1 cut(s) 568
Hpy188I TCNGA 3 cut(s) 17, 311, 414
Hpy188III TCNNGA 3 cut(s) 46, 97, 111
Hpy8I GTNNAC 1 cut(s) 568
HpyAV CCTTC 2 cut(s) 383, 420
HpyCH4III ACNGT 3 cut(s) 89, 125, 436
HpyCH4IV ACGT 1 cut(s) 570
HpyF10VI GCNNNNNNNGC 4 cut(s) 186, 248, 349, 477
HpyF3I CTNAG 3 cut(s) 143, 413, 513
HpySE526I ACGT 1 cut(s) 570
Hsp92II CATG 1 cut(s) 152
KroI GCCGGC 1 cut(s) 80
KroNI GCCGGC 1 cut(s) 82
Kzo9I GATC 1 cut(s) 296
LmnI GCTCC 3 cut(s) 220, 289, 474
LpnPI CCDG 6 cut(s) 94, 278, 295, 300, 500, 525
Lsp1109I GCAGC 1 cut(s) 198
LweI GCATC 2 cut(s) 447, 535
MaeI CTAG 3 cut(s) 305, 353, 495
MaeII ACGT 1 cut(s) 570
MaeIII GTNAC 1 cut(s) 89
MalI GATC 1 cut(s) 298
MbiI CCGCTC 1 cut(s) 477
MboI GATC 1 cut(s) 296
MboII GAAGA 2 cut(s) 44, 49
MflI RGATCY 1 cut(s) 296
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 43, 511
MmeI TCCRAC 1 cut(s) 289
MnlI CCTC 7 cut(s) 23, 71, 93, 205, 244, 349, 444
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 80
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 65, 534
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 180
MspI CCGG 4 cut(s) 81, 282, 287, 487
MwoI GCNNNNNNNGC 4 cut(s) 186, 248, 349, 477
NaeI GCCGGC 1 cut(s) 82
NdeII GATC 1 cut(s) 296
NgoMIV GCCGGC 1 cut(s) 80
NlaIII CATG 1 cut(s) 152
NlaIV GGNNCC 3 cut(s) 79, 216, 298
NmeAIII GCCGAG 1 cut(s) 235
NmuCI GTSAC 1 cut(s) 89
PaeR7I CTCGAG 2 cut(s) 97, 111
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PdiI GCCGGC 1 cut(s) 82
PflFI GACNNNGTC 1 cut(s) 173
PkrI GCNGC 2 cut(s) 188, 479
PleI GAGTC 2 cut(s) 43, 511
PpsI GAGTC 2 cut(s) 43, 511
PspFI CCCAGC 1 cut(s) 292
PspN4I GGNNCC 3 cut(s) 79, 216, 298
PspPI GGNCC 2 cut(s) 102, 284
PsuI RGATCY 1 cut(s) 296
PsyI GACNNNGTC 1 cut(s) 173
RsaI GTAC 1 cut(s) 153
RsaNI GTAC 1 cut(s) 152
Rsr2I CGGWCCG 1 cut(s) 284
RsrII CGGWCCG 1 cut(s) 284
SaqAI TTAA 2 cut(s) 65, 534
SatI GCNGC 2 cut(s) 187, 478
Sau3AI GATC 1 cut(s) 296
Sau96I GGNCC 2 cut(s) 102, 284
SchI GAGTC 2 cut(s) 43, 511
SetI ASST 6 cut(s) 244, 294, 306, 354, 528, 573
SfaNI GCATC 2 cut(s) 447, 535
Sfr274I CTCGAG 2 cut(s) 97, 111
SinI GGWCC 1 cut(s) 284
SlaI CTCGAG 2 cut(s) 97, 111
SmlI CTYRAG 2 cut(s) 97, 111
SmoI CTYRAG 2 cut(s) 97, 111
SsiI CCGC 5 cut(s) 180, 251, 444, 471, 477
SspMI CTAG 3 cut(s) 305, 353, 495
TaaI ACNGT 3 cut(s) 89, 125, 436
TaiI ACGT 1 cut(s) 573
TaqI TCGA 2 cut(s) 98, 112
TatI WGTACW 1 cut(s) 151
TauI GCSGC 1 cut(s) 480
Tru1I TTAA 2 cut(s) 65, 534
Tru9I TTAA 2 cut(s) 65, 534
TscAI CASTG 2 cut(s) 441, 544
TseFI GTSAC 1 cut(s) 89
TseI GCWGC 1 cut(s) 186
Tsp45I GTSAC 1 cut(s) 89
TspRI CASTG 2 cut(s) 441, 544
Tth111I GACNNNGTC 1 cut(s) 173
VpaK11BI GGWCC 1 cut(s) 284
XhoI CTCGAG 2 cut(s) 97, 111
XmiI GTMKAC 1 cut(s) 567
XspI CTAG 3 cut(s) 305, 353, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.