Rroxscaffold_6G00402830

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0003503
Physical Location & Seq
Reverse (-)
25304814 .. 25305407
594 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00402830.1

Sequence Viewer

Length: 594 bp
ATGACCAAAAAGTCCAAAACCCTCCCACTACTCGATTCTACTTTCCTCATTTCCCTCACCATTTGCTTCTTCACAATCCATGCCACTGCAAAATCCTCAATCTTCTCAGAAAACACATCTCCATTGACACTTGGGATCAACCAAGAGAAGCTAAGCCACCTCCACTTCTACTTCCACGATATCGCCAGCGGTCCCAACCCAACTTCCATCTGGGTTGCCCGAGCACCCACGTCTAGCAAATCTCCGACGCTGTTTGGCTCTGTAGCTATGATCGATGATGCGTTAACTATCGGCCCCAAAAGGAGCTCCAAAGTTGTGGGAAGGGCACAAGGGTTTTATGCATCTGCCTCACAAAGTGAAAATGCTTTGATGGTGACCATGAACTTGGCTTTCACCGAAGGGAAGTTTAATGGCAGCAGTCTTACTGTTGTGGGACGAAACCCTGTGGTTGAAGCTGTAAGAGAGATGCCTATAATCGGTGGAAGTGGAGTTTTTCGGTTTTCTAGGGGATATGTTCAGGCCAAGACTCTCAGGTTCAATGCAACAACCGGAGATGCTGTTGTTGAATATAATGTGTTTGTGTTCCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

197

Amino Acids

21.38

Weight (kDa)

9.59

Isoelectric Point (pI)

39.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 52 - 195 7.4e-58 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 10
AciI CCGC 1 cut(s) 189
AclWI GGATC 1 cut(s) 143
AdeI CACNNNGTG 1 cut(s) 356
AfiI CCNNNNNNNGG 1 cut(s) 476
AgsI TTSAA 3 cut(s) 452, 538, 566
AjiI CACGTC 1 cut(s) 231
AluBI AGCT 4 cut(s) 151, 266, 306, 455
AluI AGCT 4 cut(s) 151, 266, 306, 455
Alw21I GWGCWC 2 cut(s) 226, 308
AlwI GGATC 1 cut(s) 143
Ama87I CYCGRG 1 cut(s) 219
AoxI GGCC 2 cut(s) 292, 519
ApeKI GCWGC 1 cut(s) 414
AspS9I GGNCC 2 cut(s) 191, 293
AsuHPI GGTGA 3 cut(s) 49, 385, 385
AvaI CYCGRG 1 cut(s) 219
AvaII GGWCC 1 cut(s) 191
BaeGI GKGCMC 1 cut(s) 328
BanII GRGCYC 1 cut(s) 308
Bbv12I GWGCWC 2 cut(s) 226, 308
BbvI GCAGC 1 cut(s) 426
BccI CCATC 2 cut(s) 215, 364
BfaI CTAG 2 cut(s) 234, 504
BfmI CTRYAG 1 cut(s) 261
BisI GCNGC 1 cut(s) 415
BlpI GCTNAGC 1 cut(s) 152
BlsI GCNGC 1 cut(s) 416
Bme18I GGWCC 1 cut(s) 191
BmeT110I CYCGRG 1 cut(s) 219
BmgBI CACGTC 1 cut(s) 231
BmgT120I GGNCC 2 cut(s) 191, 293
BmiI GGNNCC 2 cut(s) 193, 295
BmsI GCATC 4 cut(s) 268, 350, 456, 544
Bpu1102I GCTNAGC 1 cut(s) 152
Bsa29I ATCGAT 1 cut(s) 273
BsaWI WCCGGW 1 cut(s) 548
BsaXI ACNNNNNCTCC 2 cut(s) 543, 573
Bsc4I CCNNNNNNNGG 1 cut(s) 476
BseCI ATCGAT 1 cut(s) 273
BseLI CCNNNNNNNGG 1 cut(s) 476
BseMII CTCAG 2 cut(s) 120, 544
BseSI GKGCMC 1 cut(s) 328
BseXI GCAGC 1 cut(s) 426
BshFI GGCC 2 cut(s) 294, 521
BshVI ATCGAT 1 cut(s) 273
BsiHKAI GWGCWC 2 cut(s) 226, 308
BsiHKCI CYCGRG 1 cut(s) 219
BsiSI CCGG 1 cut(s) 549
BslFI GGGAC 2 cut(s) 177, 447
BslI CCNNNNNNNGG 1 cut(s) 476
BsmFI GGGAC 2 cut(s) 177, 447
BsnI GGCC 2 cut(s) 294, 521
BsoBI CYCGRG 1 cut(s) 219
Bsp1286I GDGCHC 3 cut(s) 226, 308, 328
Bsp143I GATC 2 cut(s) 135, 270
Bsp1720I GCTNAGC 1 cut(s) 152
BspACI CCGC 1 cut(s) 189
BspANI GGCC 2 cut(s) 294, 521
BspCNI CTCAG 2 cut(s) 119, 543
BspDI ATCGAT 1 cut(s) 273
BspLI GGNNCC 2 cut(s) 193, 295
BspPI GGATC 1 cut(s) 143
BssMI GATC 2 cut(s) 135, 270
Bst4CI ACNGT 1 cut(s) 427
BstC8I GCNNGC 1 cut(s) 187
BstDEI CTNAG 3 cut(s) 106, 152, 530
BstEII GGTNACC 1 cut(s) 373
BstKTI GATC 2 cut(s) 138, 273
BstMBI GATC 2 cut(s) 135, 270
BstPI GGTNACC 1 cut(s) 373
BstSFI CTRYAG 1 cut(s) 261
BstSLI GKGCMC 1 cut(s) 328
BstV1I GCAGC 1 cut(s) 426
BstXI CCANNNNNNTGG 2 cut(s) 316, 385
Bsu15I ATCGAT 1 cut(s) 273
BsuRI GGCC 2 cut(s) 294, 521
BsuTUI ATCGAT 1 cut(s) 273
BtrI CACGTC 1 cut(s) 231
BtsI GCAGTG 1 cut(s) 84
BtsIMutI CAGTG 1 cut(s) 84
Cac8I GCNNGC 1 cut(s) 187
Cfr13I GGNCC 2 cut(s) 191, 293
ClaI ATCGAT 1 cut(s) 273
CseI GACGC 1 cut(s) 256
CviAII CATG 2 cut(s) 80, 379
CviJI RGCY 9 cut(s) 151, 156, 258, 266, 294, 306, 389, 455, 521
CviKI_1 RGCY 9 cut(s) 151, 156, 258, 266, 294, 306, 389, 455, 521
DdeI CTNAG 3 cut(s) 106, 152, 530
DpnI GATC 2 cut(s) 137, 272
DpnII GATC 2 cut(s) 135, 270
DraIII CACNNNGTG 1 cut(s) 356
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
Ecl136II GAGCTC 1 cut(s) 306
Eco24I GRGCYC 1 cut(s) 308
Eco32I GATATC 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 191
Eco53kI GAGCTC 1 cut(s) 306
Eco88I CYCGRG 1 cut(s) 219
Eco91I GGTNACC 1 cut(s) 373
EcoICRI GAGCTC 1 cut(s) 306
EcoO65I GGTNACC 1 cut(s) 373
EcoRV GATATC 1 cut(s) 181
EcoT22I ATGCAT 1 cut(s) 343
EcoT38I GRGCYC 1 cut(s) 308
FaeI CATG 2 cut(s) 83, 382
FaiI YATR 7 cut(s) 81, 269, 339, 380, 473, 513, 570
FaqI GGGAC 2 cut(s) 177, 447
FatI CATG 2 cut(s) 79, 378
Fnu4HI GCNGC 1 cut(s) 415
FriOI GRGCYC 1 cut(s) 308
Fsp4HI GCNGC 1 cut(s) 415
FspBI CTAG 2 cut(s) 234, 504
GluI GCNGC 1 cut(s) 415
HaeIII GGCC 2 cut(s) 294, 521
HapII CCGG 1 cut(s) 549
HgaI GACGC 1 cut(s) 256
Hin1II CATG 2 cut(s) 83, 382
HincII GTYRAC 1 cut(s) 285
HindII GTYRAC 1 cut(s) 285
HinfI GANTC 2 cut(s) 35, 526
HpaI GTTAAC 1 cut(s) 285
HpaII CCGG 1 cut(s) 549
HphI GGTGA 3 cut(s) 49, 385, 385
Hpy166II GTNNAC 1 cut(s) 285
Hpy188I TCNGA 2 cut(s) 109, 246
Hpy8I GTNNAC 1 cut(s) 285
Hpy99I CGWCG 1 cut(s) 250
HpyAV CCTTC 2 cut(s) 315, 392
HpyCH4III ACNGT 1 cut(s) 427
HpyCH4IV ACGT 1 cut(s) 230
HpyCH4V TGCA 3 cut(s) 89, 341, 542
HpyF3I CTNAG 3 cut(s) 106, 152, 530
HpySE526I ACGT 1 cut(s) 230
Hsp92II CATG 2 cut(s) 83, 382
KspAI GTTAAC 1 cut(s) 285
Kzo9I GATC 2 cut(s) 135, 270
LmnI GCTCC 2 cut(s) 303, 311
LpnPI CCDG 6 cut(s) 196, 199, 456, 503, 517, 562
Lsp1109I GCAGC 1 cut(s) 426
LweI GCATC 4 cut(s) 268, 350, 456, 544
MaeI CTAG 2 cut(s) 234, 504
MaeII ACGT 1 cut(s) 230
MaeIII GTNAC 1 cut(s) 373
MalI GATC 2 cut(s) 137, 272
MboI GATC 2 cut(s) 135, 270
MboII GAAGA 2 cut(s) 61, 94
MhlI GDGCHC 3 cut(s) 226, 308, 328
MlyI GAGTC 1 cut(s) 520
MmeI TCCRAC 1 cut(s) 269
MnlI CCTC 6 cut(s) 32, 56, 65, 106, 170, 358
Mph1103I ATGCAT 1 cut(s) 343
MseI TTAA 2 cut(s) 284, 408
MspA1I CMGCKG 1 cut(s) 189
MspI CCGG 1 cut(s) 549
NdeII GATC 2 cut(s) 135, 270
NlaIII CATG 2 cut(s) 83, 382
NlaIV GGNNCC 2 cut(s) 193, 295
NmuCI GTSAC 1 cut(s) 373
NsiI ATGCAT 1 cut(s) 343
PfeI GAWTC 1 cut(s) 35
PkrI GCNGC 1 cut(s) 416
PleI GAGTC 1 cut(s) 520
PpsI GAGTC 1 cut(s) 520
Psp124BI GAGCTC 1 cut(s) 308
PspEI GGTNACC 1 cut(s) 373
PspN4I GGNNCC 2 cut(s) 193, 295
PspPI GGNCC 2 cut(s) 191, 293
SacI GAGCTC 1 cut(s) 308
SaqAI TTAA 2 cut(s) 284, 408
SatI GCNGC 1 cut(s) 415
Sau3AI GATC 2 cut(s) 135, 270
Sau96I GGNCC 2 cut(s) 191, 293
SchI GAGTC 1 cut(s) 520
SduI GDGCHC 3 cut(s) 226, 308, 328
SetI ASST 7 cut(s) 153, 162, 233, 268, 308, 457, 536
SfaNI GCATC 4 cut(s) 268, 350, 456, 544
SfcI CTRYAG 1 cut(s) 261
SinI GGWCC 1 cut(s) 191
SsiI CCGC 1 cut(s) 189
SspMI CTAG 2 cut(s) 234, 504
SstI GAGCTC 1 cut(s) 308
TaaI ACNGT 1 cut(s) 427
TaiI ACGT 1 cut(s) 233
TaqI TCGA 2 cut(s) 33, 273
TfiI GAWTC 1 cut(s) 35
Tru1I TTAA 2 cut(s) 284, 408
Tru9I TTAA 2 cut(s) 284, 408
TscAI CASTG 1 cut(s) 91
TseFI GTSAC 1 cut(s) 373
TseI GCWGC 1 cut(s) 414
Tsp45I GTSAC 1 cut(s) 373
TspDTI ATGAA 1 cut(s) 395
TspRI CASTG 1 cut(s) 91
VpaK11BI GGWCC 1 cut(s) 191
XcmI CCANNNNNNNNNTGG 1 cut(s) 207
XspI CTAG 2 cut(s) 234, 504
Zsp2I ATGCAT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.