MD14G1052500.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003503
Physical Location & Seq
Forward (+)
5072434 .. 5072982
549 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1052500.v1.1.491

Sequence Viewer

Length: 549 bp
ATGGCCAAAACCTCCCAAACCCTCCCAGATCTACCATCTACCCTACTCATATTTTCCTTCACTGTTCTCTTCTCTACAACCCTAGGGCTCAAAAACCAAGAAAAGCTAAGCCACCTCCATTTCTACTTCCATGACATAGTCAGCGGTCCAAACCCTGCTGCCGTCTGGGTTGCCCAAACACCCATCTCCAAAAAATCTCCAACCCTATTCGGCTCTATAGCCATGTTCGACGACCCGCTAACCGTAGGCCCCACAAGGAGCTCCAAACTTGTGGGGAGAGCACAGGGGTTATACGGGTCAGCCTCACAGAGTGAAGATGCTTTGTTGATGGCCATGAACTTCGTATTTTATGAAGGCAATTATAATGGGAGCAGTCTCAGTGTGCTGGGTCGAAACTCCGTGCTTGAAGCCGTGAGAGAGATGCCGGTAATCGGTGGAAGTGGGGTTTTCCGGCTGGCTCGCGGGTATGTTCAGGCGAAGACTGTCACGTTTAATGCAACTAGTGGGGATGCTCTTGTTGAATATGATGCGTTTGTCTTCCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

183

Amino Acids

19.71

Weight (kDa)

6.9

Isoelectric Point (pI)

34.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 37 - 180 4.2e-57 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 363
AccII CGCG 1 cut(s) 462
AciI CCGC 3 cut(s) 144, 236, 462
AcoI YGGCCR 2 cut(s) 3, 330
AdeI CACNNNGTG 1 cut(s) 311
AfiI CCNNNNNNNGG 1 cut(s) 431
AgsI TTSAA 2 cut(s) 407, 521
AhlI ACTAGT 1 cut(s) 500
AluBI AGCT 2 cut(s) 106, 261
AluI AGCT 2 cut(s) 106, 261
Alw21I GWGCWC 2 cut(s) 263, 283
Alw26I GTCTC 1 cut(s) 380
AoxI GGCC 3 cut(s) 3, 247, 330
ApeKI GCWGC 1 cut(s) 158
AspA2I CCTAGG 1 cut(s) 82
AspS9I GGNCC 2 cut(s) 146, 248
AvaII GGWCC 1 cut(s) 146
AvrII CCTAGG 1 cut(s) 82
BalI TGGCCA 2 cut(s) 5, 332
BanII GRGCYC 2 cut(s) 90, 263
BbsI GAAGAC 2 cut(s) 485, 529
Bbv12I GWGCWC 2 cut(s) 263, 283
BbvI GCAGC 1 cut(s) 145
BccI CCATC 3 cut(s) 43, 191, 322
BceAI ACGGC 2 cut(s) 146, 395
BcoDI GTCTC 1 cut(s) 380
BcuI ACTAGT 1 cut(s) 500
BfaI CTAG 2 cut(s) 83, 501
BfmI CTRYAG 1 cut(s) 216
BglII AGATCT 1 cut(s) 28
BisI GCNGC 1 cut(s) 159
BlnI CCTAGG 1 cut(s) 82
BlpI GCTNAGC 1 cut(s) 107
BlsI GCNGC 1 cut(s) 160
Bme18I GGWCC 1 cut(s) 146
BmgT120I GGNCC 2 cut(s) 146, 248
BmiI GGNNCC 1 cut(s) 250
BmsI GCATC 4 cut(s) 307, 411, 499, 517
BpiI GAAGAC 2 cut(s) 485, 529
Bpu1102I GCTNAGC 1 cut(s) 107
BsaJI CCNNGG 1 cut(s) 82
Bsc4I CCNNNNNNNGG 1 cut(s) 431
Bse118I RCCGGY 1 cut(s) 424
BseDI CCNNGG 1 cut(s) 82
BseGI GGATG 1 cut(s) 514
BseLI CCNNNNNNNGG 1 cut(s) 431
BseMII CTCAG 1 cut(s) 391
BseXI GCAGC 1 cut(s) 145
BseYI CCCAGC 1 cut(s) 385
Bsh1236I CGCG 1 cut(s) 462
BshFI GGCC 3 cut(s) 5, 249, 332
BsiHKAI GWGCWC 2 cut(s) 263, 283
BsiSI CCGG 2 cut(s) 425, 451
BslI CCNNNNNNNGG 1 cut(s) 431
BsmAI GTCTC 1 cut(s) 380
BsnI GGCC 3 cut(s) 5, 249, 332
Bsp1286I GDGCHC 3 cut(s) 90, 263, 283
Bsp143I GATC 1 cut(s) 28
Bsp1720I GCTNAGC 1 cut(s) 107
BspACI CCGC 3 cut(s) 144, 236, 462
BspANI GGCC 3 cut(s) 5, 249, 332
BspCNI CTCAG 1 cut(s) 390
BspFNI CGCG 1 cut(s) 462
BspLI GGNNCC 1 cut(s) 250
BsrFI RCCGGY 1 cut(s) 424
BssAI RCCGGY 1 cut(s) 424
BssECI CCNNGG 1 cut(s) 82
BssMI GATC 1 cut(s) 28
BssT1I CCWWGG 1 cut(s) 82
Bst4CI ACNGT 3 cut(s) 64, 244, 484
Bst6I CTCTTC 1 cut(s) 74
BstC8I GCNNGC 2 cut(s) 456, 460
BstDEI CTNAG 2 cut(s) 107, 377
BstF5I GGATG 1 cut(s) 514
BstFNI CGCG 1 cut(s) 462
BstKTI GATC 1 cut(s) 31
BstMAI GTCTC 1 cut(s) 380
BstMBI GATC 1 cut(s) 28
BstSFI CTRYAG 1 cut(s) 216
BstUI CGCG 1 cut(s) 462
BstV1I GCAGC 1 cut(s) 145
BstV2I GAAGAC 2 cut(s) 485, 529
BstX2I RGATCY 1 cut(s) 28
BstXI CCANNNNNNTGG 1 cut(s) 271
BstYI RGATCY 1 cut(s) 28
BsuRI GGCC 3 cut(s) 5, 249, 332
BtsCI GGATG 1 cut(s) 514
BtsIMutI CAGTG 2 cut(s) 60, 385
Cac8I GCNNGC 2 cut(s) 456, 460
Cfr10I RCCGGY 1 cut(s) 424
Cfr13I GGNCC 2 cut(s) 146, 248
CviAII CATG 3 cut(s) 131, 223, 334
DdeI CTNAG 2 cut(s) 107, 377
DpnI GATC 1 cut(s) 30
DpnII GATC 1 cut(s) 28
DraIII CACNNNGTG 1 cut(s) 311
EaeI YGGCCR 2 cut(s) 3, 330
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
Ecl136II GAGCTC 1 cut(s) 261
Eco130I CCWWGG 1 cut(s) 82
Eco24I GRGCYC 2 cut(s) 90, 263
Eco47I GGWCC 1 cut(s) 146
Eco53kI GAGCTC 1 cut(s) 261
EcoICRI GAGCTC 1 cut(s) 261
EcoO109I RGGNCCY 1 cut(s) 248
EcoT14I CCWWGG 1 cut(s) 82
EcoT38I GRGCYC 2 cut(s) 90, 263
ErhI CCWWGG 1 cut(s) 82
FaeI CATG 3 cut(s) 134, 226, 337
FatI CATG 3 cut(s) 130, 222, 333
FauI CCCGC 2 cut(s) 243, 455
Fnu4HI GCNGC 1 cut(s) 159
FokI GGATG 1 cut(s) 521
FriOI GRGCYC 2 cut(s) 90, 263
Fsp4HI GCNGC 1 cut(s) 159
FspBI CTAG 2 cut(s) 83, 501
GluI GCNGC 1 cut(s) 159
GsaI CCCAGC 1 cut(s) 389
HaeIII GGCC 3 cut(s) 5, 249, 332
HapII CCGG 2 cut(s) 425, 451
Hin1II CATG 3 cut(s) 134, 226, 337
HpaII CCGG 2 cut(s) 425, 451
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 2 cut(s) 67, 347
HpyCH4III ACNGT 3 cut(s) 64, 244, 484
HpyCH4IV ACGT 1 cut(s) 488
HpyCH4V TGCA 1 cut(s) 497
HpyF3I CTNAG 2 cut(s) 107, 377
HpySE526I ACGT 1 cut(s) 488
Hsp92II CATG 3 cut(s) 134, 226, 337
Kzo9I GATC 1 cut(s) 28
LmnI GCTCC 3 cut(s) 258, 266, 369
LpnPI CCDG 9 cut(s) 39, 151, 168, 269, 371, 438, 440, 458, 464
Lsp1109I GCAGC 1 cut(s) 145
LweI GCATC 4 cut(s) 307, 411, 499, 517
MaeI CTAG 2 cut(s) 83, 501
MaeII ACGT 1 cut(s) 488
MaeIII GTNAC 1 cut(s) 484
MalI GATC 1 cut(s) 30
MboI GATC 1 cut(s) 28
MboII GAAGA 4 cut(s) 61, 326, 490, 529
MflI RGATCY 1 cut(s) 28
MhlI GDGCHC 3 cut(s) 90, 263, 283
MlsI TGGCCA 2 cut(s) 5, 332
MluCI AATT 1 cut(s) 358
MluNI TGGCCA 2 cut(s) 5, 332
MmeI TCCRAC 1 cut(s) 224
MnlI CCTC 4 cut(s) 22, 32, 125, 313
Mox20I TGGCCA 2 cut(s) 5, 332
MscI TGGCCA 2 cut(s) 5, 332
MseI TTAA 1 cut(s) 492
Msp20I TGGCCA 2 cut(s) 5, 332
MspA1I CMGCKG 1 cut(s) 144
MspI CCGG 2 cut(s) 425, 451
MvnI CGCG 1 cut(s) 462
NdeII GATC 1 cut(s) 28
NlaIII CATG 3 cut(s) 134, 226, 337
NlaIV GGNNCC 1 cut(s) 250
NmuCI GTSAC 1 cut(s) 484
PflFI GACNNNGTC 1 cut(s) 137
PkrI GCNGC 1 cut(s) 160
PsiI TTATAA 1 cut(s) 363
Psp124BI GAGCTC 1 cut(s) 263
PspFI CCCAGC 1 cut(s) 385
PspN4I GGNNCC 1 cut(s) 250
PspPI GGNCC 2 cut(s) 146, 248
PsuI RGATCY 1 cut(s) 28
PsyI GACNNNGTC 1 cut(s) 137
SacI GAGCTC 1 cut(s) 263
SaqAI TTAA 1 cut(s) 492
SatI GCNGC 1 cut(s) 159
Sau3AI GATC 1 cut(s) 28
Sau96I GGNCC 2 cut(s) 146, 248
SduI GDGCHC 3 cut(s) 90, 263, 283
SetI ASST 5 cut(s) 14, 108, 117, 263, 491
SfaNI GCATC 4 cut(s) 307, 411, 499, 517
SfcI CTRYAG 1 cut(s) 216
SinI GGWCC 1 cut(s) 146
SpeI ACTAGT 1 cut(s) 500
Sse9I AATT 1 cut(s) 358
SsiI CCGC 3 cut(s) 144, 236, 462
SspMI CTAG 2 cut(s) 83, 501
SstI GAGCTC 1 cut(s) 263
StyI CCWWGG 1 cut(s) 82
TaaI ACNGT 3 cut(s) 64, 244, 484
TaiI ACGT 1 cut(s) 491
TaqI TCGA 2 cut(s) 228, 391
TasI AATT 1 cut(s) 358
Tru1I TTAA 1 cut(s) 492
Tru9I TTAA 1 cut(s) 492
TscAI CASTG 2 cut(s) 67, 385
TseFI GTSAC 1 cut(s) 484
TseI GCWGC 1 cut(s) 158
Tsp45I GTSAC 1 cut(s) 484
TspDTI ATGAA 2 cut(s) 350, 366
TspGWI ACGGA 1 cut(s) 388
TspRI CASTG 2 cut(s) 67, 385
Tth111I GACNNNGTC 1 cut(s) 137
VpaK11BI GGWCC 1 cut(s) 146
XmaJI CCTAGG 1 cut(s) 82
XspI CTAG 2 cut(s) 83, 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.