Rh3AG221400

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0003503
Physical Location & Seq
Forward (+)
22727141 .. 22727636
496 bp
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UTR
Exon/CDS
Intron
Rh3AG221400.1

Sequence Viewer

Length: 315 bp
ATGATCGATGATGCGTTAACTATCGGCCCCAAAAGGAGCTCCAAAGTTGTGGGAAGGGCACAAGGGTTTTATGCATCTGCCTCACAAAGTGAAAATGCTTTGATGGTGACCATGAACTTGGCTTTCACCGAAGGGAAGTTTAATGGCAGCAGTCTTACTGTTGTGGGACGAAACCCTGTGGTTGAAGCTGTAAGAGAGATGCCTATAATCGGTGGAAGTGGAGTTTTTCGGTTTTCTAGGGGATATGTTCAGGCCAAGACTCTCAGGTTCAATGCAACAACCGGAGATGCTGTTGTTGAATATAATCCAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

104

Amino Acids

11.08

Weight (kDa)

9.59

Isoelectric Point (pI)

41.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 1 - 102 1.5e-40 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AdeI CACNNNGTG 1 cut(s) 89
AfiI CCNNNNNNNGG 1 cut(s) 209
AgsI TTSAA 3 cut(s) 185, 271, 299
AluBI AGCT 2 cut(s) 39, 188
AluI AGCT 2 cut(s) 39, 188
Alw21I GWGCWC 1 cut(s) 41
AoxI GGCC 2 cut(s) 25, 252
ApeKI GCWGC 1 cut(s) 147
AspS9I GGNCC 1 cut(s) 26
AsuHPI GGTGA 2 cut(s) 118, 118
BaeGI GKGCMC 1 cut(s) 61
BanII GRGCYC 1 cut(s) 41
Bbv12I GWGCWC 1 cut(s) 41
BbvI GCAGC 1 cut(s) 159
BccI CCATC 1 cut(s) 97
BfaI CTAG 1 cut(s) 237
BisI GCNGC 1 cut(s) 148
BlsI GCNGC 1 cut(s) 149
BmgT120I GGNCC 1 cut(s) 26
BmiI GGNNCC 1 cut(s) 28
BmsI GCATC 3 cut(s) 83, 189, 277
Bsa29I ATCGAT 1 cut(s) 6
BsaWI WCCGGW 1 cut(s) 281
BsaXI ACNNNNNCTCC 2 cut(s) 276, 306
Bsc4I CCNNNNNNNGG 1 cut(s) 209
BseCI ATCGAT 1 cut(s) 6
BseLI CCNNNNNNNGG 1 cut(s) 209
BseMII CTCAG 1 cut(s) 277
BseSI GKGCMC 1 cut(s) 61
BseXI GCAGC 1 cut(s) 159
BshFI GGCC 2 cut(s) 27, 254
BshVI ATCGAT 1 cut(s) 6
BsiHKAI GWGCWC 1 cut(s) 41
BsiSI CCGG 1 cut(s) 282
BslFI GGGAC 1 cut(s) 180
BslI CCNNNNNNNGG 1 cut(s) 209
BsmFI GGGAC 1 cut(s) 180
BsnI GGCC 2 cut(s) 27, 254
Bsp1286I GDGCHC 2 cut(s) 41, 61
Bsp143I GATC 1 cut(s) 3
BspANI GGCC 2 cut(s) 27, 254
BspCNI CTCAG 1 cut(s) 276
BspDI ATCGAT 1 cut(s) 6
BspLI GGNNCC 1 cut(s) 28
BssMI GATC 1 cut(s) 3
Bst4CI ACNGT 1 cut(s) 160
BstDEI CTNAG 1 cut(s) 263
BstEII GGTNACC 1 cut(s) 106
BstKTI GATC 1 cut(s) 6
BstMBI GATC 1 cut(s) 3
BstPI GGTNACC 1 cut(s) 106
BstSLI GKGCMC 1 cut(s) 61
BstV1I GCAGC 1 cut(s) 159
BstXI CCANNNNNNTGG 2 cut(s) 49, 118
Bsu15I ATCGAT 1 cut(s) 6
BsuRI GGCC 2 cut(s) 27, 254
BsuTUI ATCGAT 1 cut(s) 6
Cfr13I GGNCC 1 cut(s) 26
ClaI ATCGAT 1 cut(s) 6
CviAII CATG 1 cut(s) 112
CviJI RGCY 5 cut(s) 27, 39, 122, 188, 254
CviKI_1 RGCY 5 cut(s) 27, 39, 122, 188, 254
DdeI CTNAG 1 cut(s) 263
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
DraIII CACNNNGTG 1 cut(s) 89
Ecl136II GAGCTC 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 41
Eco53kI GAGCTC 1 cut(s) 39
Eco91I GGTNACC 1 cut(s) 106
EcoICRI GAGCTC 1 cut(s) 39
EcoO65I GGTNACC 1 cut(s) 106
EcoT22I ATGCAT 1 cut(s) 76
EcoT38I GRGCYC 1 cut(s) 41
FaeI CATG 1 cut(s) 115
FaiI YATR 5 cut(s) 72, 113, 206, 246, 303
FaqI GGGAC 1 cut(s) 180
FatI CATG 1 cut(s) 111
Fnu4HI GCNGC 1 cut(s) 148
FriOI GRGCYC 1 cut(s) 41
Fsp4HI GCNGC 1 cut(s) 148
FspBI CTAG 1 cut(s) 237
GluI GCNGC 1 cut(s) 148
HaeIII GGCC 2 cut(s) 27, 254
HapII CCGG 1 cut(s) 282
Hin1II CATG 1 cut(s) 115
HincII GTYRAC 1 cut(s) 18
HindII GTYRAC 1 cut(s) 18
HinfI GANTC 1 cut(s) 259
HpaI GTTAAC 1 cut(s) 18
HpaII CCGG 1 cut(s) 282
HphI GGTGA 2 cut(s) 118, 118
Hpy166II GTNNAC 1 cut(s) 18
Hpy8I GTNNAC 1 cut(s) 18
HpyAV CCTTC 2 cut(s) 48, 125
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4V TGCA 2 cut(s) 74, 275
HpyF3I CTNAG 1 cut(s) 263
Hsp92II CATG 1 cut(s) 115
KspAI GTTAAC 1 cut(s) 18
Kzo9I GATC 1 cut(s) 3
LmnI GCTCC 2 cut(s) 36, 44
LpnPI CCDG 4 cut(s) 189, 236, 250, 295
Lsp1109I GCAGC 1 cut(s) 159
LweI GCATC 3 cut(s) 83, 189, 277
MaeI CTAG 1 cut(s) 237
MaeIII GTNAC 1 cut(s) 106
MalI GATC 1 cut(s) 5
MboI GATC 1 cut(s) 3
MhlI GDGCHC 2 cut(s) 41, 61
MlyI GAGTC 1 cut(s) 253
MnlI CCTC 1 cut(s) 91
Mph1103I ATGCAT 1 cut(s) 76
MseI TTAA 2 cut(s) 17, 141
MspI CCGG 1 cut(s) 282
NdeII GATC 1 cut(s) 3
NlaIII CATG 1 cut(s) 115
NlaIV GGNNCC 1 cut(s) 28
NmuCI GTSAC 1 cut(s) 106
NsiI ATGCAT 1 cut(s) 76
PkrI GCNGC 1 cut(s) 149
PleI GAGTC 1 cut(s) 253
PpsI GAGTC 1 cut(s) 253
Psp124BI GAGCTC 1 cut(s) 41
PspEI GGTNACC 1 cut(s) 106
PspN4I GGNNCC 1 cut(s) 28
PspPI GGNCC 1 cut(s) 26
SacI GAGCTC 1 cut(s) 41
SaqAI TTAA 2 cut(s) 17, 141
SatI GCNGC 1 cut(s) 148
Sau3AI GATC 1 cut(s) 3
Sau96I GGNCC 1 cut(s) 26
SchI GAGTC 1 cut(s) 253
SduI GDGCHC 2 cut(s) 41, 61
SetI ASST 3 cut(s) 41, 190, 269
SfaNI GCATC 3 cut(s) 83, 189, 277
SgeI CNNG 9 cut(s) 74, 124, 130, 188, 249, 263, 268, 277, 294
SspMI CTAG 1 cut(s) 237
SstI GAGCTC 1 cut(s) 41
TaaI ACNGT 1 cut(s) 160
TaqI TCGA 1 cut(s) 6
Tru1I TTAA 2 cut(s) 17, 141
Tru9I TTAA 2 cut(s) 17, 141
TseFI GTSAC 1 cut(s) 106
TseI GCWGC 1 cut(s) 147
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 1 cut(s) 128
XspI CTAG 1 cut(s) 237
Zsp2I ATGCAT 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.