Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 1626 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1444, 2011 |
| AccBSI | CCGCTC | 1 cut(s) | 287 |
| AciI | CCGC | 8 cut(s) | 97, 287, 485, 516, 686, 721, 1072, 1521 |
| AclI | AACGTT | 1 cut(s) | 2046 |
| AclWI | GGATC | 5 cut(s) | 295, 308, 516, 731, 749 |
| AcsI | RAATTY | 4 cut(s) | 67, 590, 714, 1382 |
| AcuI | CTGAAG | 1 cut(s) | 1155 |
| AcvI | CACGTG | 1 cut(s) | 1231 |
| AcyI | GRCGYC | 1 cut(s) | 1623 |
| AfaI | GTAC | 3 cut(s) | 966, 1144, 1761 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 13, 154, 359, 374, 1444, 1769, 1943, 1996, 2011 |
| AflIII | ACRYGT | 1 cut(s) | 1228 |
| AgsI | TTSAA | 7 cut(s) | 139, 337, 433, 962, 1187, 1226, 2093 |
| AjnI | CCWGG | 4 cut(s) | 541, 636, 1555, 1726 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 398, 430 |
| AleI | CACNNNNGTG | 1 cut(s) | 1103 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2036, 2068 |
| AluBI | AGCT | 6 cut(s) | 629, 950, 1432, 1486, 1721, 1847 |
| AluI | AGCT | 6 cut(s) | 629, 950, 1432, 1486, 1721, 1847 |
| Alw21I | GWGCWC | 4 cut(s) | 835, 880, 1306, 1723 |
| Alw26I | GTCTC | 3 cut(s) | 650, 674, 1123 |
| Alw44I | GTGCAC | 1 cut(s) | 876 |
| AlwI | GGATC | 5 cut(s) | 295, 308, 516, 731, 749 |
| Ama87I | CYCGRG | 2 cut(s) | 43, 1906 |
| AoxI | GGCC | 1 cut(s) | 922 |
| ApaLI | GTGCAC | 1 cut(s) | 876 |
| ApeKI | GCWGC | 4 cut(s) | 620, 1583, 1586, 1956 |
| ApoI | RAATTY | 4 cut(s) | 67, 590, 714, 1382 |
| AseI | ATTAAT | 1 cut(s) | 597 |
| AspLEI | GCGC | 2 cut(s) | 625, 2120 |
| AspS9I | GGNCC | 3 cut(s) | 4, 365, 987 |
| AsuC2I | CCSGG | 1 cut(s) | 155 |
| AsuHPI | GGTGA | 7 cut(s) | 59, 86, 531, 1029, 1117, 1883, 2019 |
| AsuNHI | GCTAGC | 1 cut(s) | 625 |
| AvaI | CYCGRG | 2 cut(s) | 43, 1906 |
| AvaII | GGWCC | 3 cut(s) | 4, 365, 987 |
| BaeGI | GKGCMC | 1 cut(s) | 880 |
| BamHI | GGATCC | 1 cut(s) | 300 |
| BanII | GRGCYC | 2 cut(s) | 1566, 1723 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 444, 476 |
| BauI | CACGAG | 1 cut(s) | 1053 |
| BbrPI | CACGTG | 1 cut(s) | 1231 |
| BbsI | GAAGAC | 1 cut(s) | 92 |
| Bbv12I | GWGCWC | 4 cut(s) | 835, 880, 1306, 1723 |
| BbvI | GCAGC | 4 cut(s) | 607, 1570, 1573, 1968 |
| BccI | CCATC | 3 cut(s) | 1305, 1438, 1922 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1561, 1595 |
| BciT130I | CCWGG | 4 cut(s) | 543, 638, 1557, 1728 |
| BcnI | CCSGG | 1 cut(s) | 155 |
| BcoDI | GTCTC | 3 cut(s) | 650, 674, 1123 |
| BfaI | CTAG | 3 cut(s) | 519, 626, 1374 |
| BfmI | CTRYAG | 1 cut(s) | 1267 |
| BglII | AGATCT | 1 cut(s) | 1273 |
| BisI | GCNGC | 6 cut(s) | 516, 621, 687, 1584, 1587, 1957 |
| BlsI | GCNGC | 6 cut(s) | 517, 622, 688, 1585, 1588, 1958 |
| BmcAI | AGTACT | 2 cut(s) | 966, 1144 |
| Bme1390I | CCNGG | 5 cut(s) | 155, 543, 638, 1557, 1728 |
| Bme18I | GGWCC | 3 cut(s) | 4, 365, 987 |
| BmeT110I | CYCGRG | 2 cut(s) | 43, 1906 |
| BmgT120I | GGNCC | 3 cut(s) | 4, 365, 987 |
| BmiI | GGNNCC | 6 cut(s) | 40, 224, 302, 988, 989, 2016 |
| BmrFI | CCNGG | 5 cut(s) | 155, 543, 638, 1557, 1728 |
| BmrI | ACTGGG | 2 cut(s) | 1560, 1953 |
| BmsI | GCATC | 4 cut(s) | 71, 895, 1166, 1193 |
| BmtI | GCTAGC | 1 cut(s) | 629 |
| BmuI | ACTGGG | 2 cut(s) | 1560, 1953 |
| BoxI | GACNNNNGTC | 1 cut(s) | 582 |
| BpiI | GAAGAC | 1 cut(s) | 92 |
| BpmI | CTGGAG | 3 cut(s) | 811, 1310, 1737 |
| BpuMI | CCSGG | 1 cut(s) | 155 |
| BsaAI | YACGTR | 1 cut(s) | 1231 |
| BsaHI | GRCGYC | 1 cut(s) | 1623 |
| BsaJI | CCNNGG | 5 cut(s) | 42, 696, 803, 1905, 2055 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 179, 209 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 13, 154, 359, 374, 1444, 1769, 1943, 1996, 2011 |
| Bse118I | RCCGGY | 1 cut(s) | 387 |
| Bse1I | ACTGG | 6 cut(s) | 304, 828, 1047, 1566, 1720, 1948 |
| Bse3DI | GCAATG | 2 cut(s) | 570, 1398 |
| BseBI | CCWGG | 4 cut(s) | 543, 638, 1557, 1728 |
| BseDI | CCNNGG | 5 cut(s) | 42, 696, 803, 1905, 2055 |
| BseGI | GGATG | 7 cut(s) | 538, 1181, 1208, 1338, 1449, 1672, 1723 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 13, 154, 359, 374, 1444, 1769, 1943, 1996, 2011 |
| BseMI | GCAATG | 2 cut(s) | 570, 1398 |
| BseMII | CTCAG | 4 cut(s) | 122, 249, 645, 1935 |
| BseNI | ACTGG | 6 cut(s) | 304, 828, 1047, 1566, 1720, 1948 |
| BseSI | GKGCMC | 1 cut(s) | 880 |
| BseXI | GCAGC | 4 cut(s) | 607, 1570, 1573, 1968 |
| BsgI | GTGCAG | 1 cut(s) | 1679 |
| BshFI | GGCC | 1 cut(s) | 924 |
| BsiHKAI | GWGCWC | 4 cut(s) | 835, 880, 1306, 1723 |
| BsiHKCI | CYCGRG | 2 cut(s) | 43, 1906 |
| BsiSI | CCGG | 2 cut(s) | 155, 388 |
| BslFI | GGGAC | 2 cut(s) | 1000, 1960 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 13, 154, 359, 374, 1444, 1769, 1943, 1996, 2011 |
| BsmAI | GTCTC | 3 cut(s) | 650, 674, 1123 |
| BsmFI | GGGAC | 2 cut(s) | 1000, 1960 |
| BsmI | GAATGC | 1 cut(s) | 427 |
| BsnI | GGCC | 1 cut(s) | 924 |
| BsoBI | CYCGRG | 2 cut(s) | 43, 1906 |
| Bsp1286I | GDGCHC | 5 cut(s) | 835, 880, 1306, 1566, 1723 |
| Bsp143I | GATC | 8 cut(s) | 210, 300, 521, 723, 741, 1154, 1273, 1973 |
| Bsp19I | CCATGG | 1 cut(s) | 696 |
| BspACI | CCGC | 8 cut(s) | 97, 287, 485, 516, 686, 721, 1072, 1521 |
| BspANI | GGCC | 1 cut(s) | 924 |
| BspCNI | CTCAG | 4 cut(s) | 121, 250, 646, 1934 |
| BspHI | TCATGA | 2 cut(s) | 586, 672 |
| BspLI | GGNNCC | 6 cut(s) | 40, 224, 302, 988, 989, 2016 |
| BspOI | GCTAGC | 1 cut(s) | 629 |
| BspPI | GGATC | 5 cut(s) | 295, 308, 516, 731, 749 |
| BspQI | GCTCTTC | 1 cut(s) | 432 |
| BsrBI | CCGCTC | 1 cut(s) | 287 |
| BsrDI | GCAATG | 2 cut(s) | 570, 1398 |
| BsrFI | RCCGGY | 1 cut(s) | 387 |
| BsrI | ACTGG | 6 cut(s) | 304, 828, 1047, 1566, 1720, 1948 |
| BssAI | RCCGGY | 1 cut(s) | 387 |
| BssECI | CCNNGG | 5 cut(s) | 42, 696, 803, 1905, 2055 |
| BssMI | GATC | 8 cut(s) | 210, 300, 521, 723, 741, 1154, 1273, 1973 |
| BssNI | GRCGYC | 1 cut(s) | 1623 |
| BssSI | CACGAG | 1 cut(s) | 1053 |
| BssT1I | CCWWGG | 2 cut(s) | 696, 803 |
| Bst2BI | CACGAG | 1 cut(s) | 1053 |
| Bst2UI | CCWGG | 4 cut(s) | 543, 638, 1557, 1728 |
| Bst4CI | ACNGT | 7 cut(s) | 195, 670, 755, 1736, 1996, 2065, 2089 |
| Bst6I | CTCTTC | 4 cut(s) | 285, 432, 696, 1888 |
| BstACI | GRCGYC | 1 cut(s) | 1623 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1397 |
| BstBAI | YACGTR | 1 cut(s) | 1231 |
| BstC8I | GCNNGC | 4 cut(s) | 627, 868, 1562, 2122 |
| BstDEI | CTNAG | 6 cut(s) | 108, 258, 581, 654, 1209, 1921 |
| BstDSI | CCRYGG | 2 cut(s) | 696, 2055 |
| BstF5I | GGATG | 7 cut(s) | 538, 1181, 1208, 1338, 1449, 1672, 1723 |
| BstHHI | GCGC | 2 cut(s) | 625, 2120 |
| BstKTI | GATC | 8 cut(s) | 213, 303, 524, 726, 744, 1157, 1276, 1976 |
| BstMAI | GTCTC | 3 cut(s) | 650, 674, 1123 |
| BstMBI | GATC | 8 cut(s) | 210, 300, 521, 723, 741, 1154, 1273, 1973 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 388, 626, 692, 1301, 1397, 1419, 1786 |
| BstNI | CCWGG | 4 cut(s) | 543, 638, 1557, 1728 |
| BstNSI | RCATGY | 2 cut(s) | 1195, 1477 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 582 |
| BstSCI | CCNGG | 5 cut(s) | 153, 541, 636, 1555, 1726 |
| BstSFI | CTRYAG | 1 cut(s) | 1267 |
| BstSLI | GKGCMC | 1 cut(s) | 880 |
| BstV1I | GCAGC | 4 cut(s) | 607, 1570, 1573, 1968 |
| BstV2I | GAAGAC | 1 cut(s) | 92 |
| BstX2I | RGATCY | 3 cut(s) | 300, 521, 1273 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1573 |
| BstYI | RGATCY | 3 cut(s) | 300, 521, 1273 |
| BsuRI | GGCC | 1 cut(s) | 924 |
| BtgI | CCRYGG | 2 cut(s) | 696, 2055 |
| BtsCI | GGATG | 7 cut(s) | 538, 1181, 1208, 1338, 1449, 1672, 1723 |
| BtsI | GCAGTG | 1 cut(s) | 1667 |
| BtsIMutI | CAGTG | 7 cut(s) | 311, 760, 835, 1040, 1573, 1667, 2001 |
| Cac8I | GCNNGC | 4 cut(s) | 627, 868, 1562, 2122 |
| CciI | TCATGA | 2 cut(s) | 586, 672 |
| CfoI | GCGC | 2 cut(s) | 625, 2120 |
| Cfr10I | RCCGGY | 1 cut(s) | 387 |
| Cfr13I | GGNCC | 3 cut(s) | 4, 365, 987 |
| CsiI | ACCWGGT | 1 cut(s) | 636 |
| Csp6I | GTAC | 3 cut(s) | 965, 1143, 1760 |
| CviQI | GTAC | 3 cut(s) | 965, 1143, 1760 |
| DdeI | CTNAG | 6 cut(s) | 108, 258, 581, 654, 1209, 1921 |
| DpnI | GATC | 8 cut(s) | 212, 302, 523, 725, 743, 1156, 1275, 1975 |
| DpnII | GATC | 8 cut(s) | 210, 300, 521, 723, 741, 1154, 1273, 1973 |
| DraI | TTTAAA | 1 cut(s) | 1135 |
| Eam1104I | CTCTTC | 4 cut(s) | 285, 432, 696, 1888 |
| EarI | CTCTTC | 4 cut(s) | 285, 432, 696, 1888 |
| Ecl136II | GAGCTC | 1 cut(s) | 1721 |
| Eco130I | CCWWGG | 2 cut(s) | 696, 803 |
| Eco147I | AGGCCT | 1 cut(s) | 924 |
| Eco24I | GRGCYC | 2 cut(s) | 1566, 1723 |
| Eco47I | GGWCC | 3 cut(s) | 4, 365, 987 |
| Eco53kI | GAGCTC | 1 cut(s) | 1721 |
| Eco57I | CTGAAG | 1 cut(s) | 1155 |
| Eco72I | CACGTG | 1 cut(s) | 1231 |
| Eco88I | CYCGRG | 2 cut(s) | 43, 1906 |
| EcoICRI | GAGCTC | 1 cut(s) | 1721 |
| EcoO109I | RGGNCCY | 2 cut(s) | 365, 987 |
| EcoRI | GAATTC | 1 cut(s) | 67 |
| EcoRII | CCWGG | 4 cut(s) | 541, 636, 1555, 1726 |
| EcoT14I | CCWWGG | 2 cut(s) | 696, 803 |
| EcoT22I | ATGCAT | 1 cut(s) | 1181 |
| EcoT38I | GRGCYC | 2 cut(s) | 1566, 1723 |
| ErhI | CCWWGG | 2 cut(s) | 696, 803 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1475, 1507 |
| FaqI | GGGAC | 2 cut(s) | 1000, 1960 |
| FauI | CCCGC | 1 cut(s) | 1514 |
| FauNDI | CATATG | 2 cut(s) | 511, 1297 |
| Fnu4HI | GCNGC | 6 cut(s) | 516, 621, 687, 1584, 1587, 1957 |
| FokI | GGATG | 7 cut(s) | 545, 1188, 1215, 1345, 1456, 1679, 1710 |
| FriOI | GRGCYC | 2 cut(s) | 1566, 1723 |
| Fsp4HI | GCNGC | 6 cut(s) | 516, 621, 687, 1584, 1587, 1957 |
| FspBI | CTAG | 3 cut(s) | 519, 626, 1374 |
| GlaI | GCGC | 2 cut(s) | 624, 2119 |
| GluI | GCNGC | 6 cut(s) | 516, 621, 687, 1584, 1587, 1957 |
| GsuI | CTGGAG | 3 cut(s) | 811, 1310, 1737 |
| HaeIII | GGCC | 1 cut(s) | 924 |
| HapII | CCGG | 2 cut(s) | 155, 388 |
| HhaI | GCGC | 2 cut(s) | 625, 2120 |
| Hin1I | GRCGYC | 1 cut(s) | 1623 |
| Hin6I | GCGC | 2 cut(s) | 623, 2118 |
| HinP1I | GCGC | 2 cut(s) | 623, 2118 |
| HincII | GTYRAC | 3 cut(s) | 507, 892, 1879 |
| HindII | GTYRAC | 3 cut(s) | 507, 892, 1879 |
| HindIII | AAGCTT | 2 cut(s) | 1430, 1484 |
| HinfI | GANTC | 9 cut(s) | 238, 250, 500, 842, 854, 1183, 1530, 1880, 2051 |
| HpaII | CCGG | 2 cut(s) | 155, 388 |
| HphI | GGTGA | 7 cut(s) | 59, 86, 531, 1029, 1117, 1883, 2019 |
| Hpy166II | GTNNAC | 5 cut(s) | 507, 878, 892, 1879, 1985 |
| Hpy188I | TCNGA | 4 cut(s) | 577, 1507, 1888, 1924 |
| Hpy8I | GTNNAC | 5 cut(s) | 507, 878, 892, 1879, 1985 |
| Hpy99I | CGWCG | 2 cut(s) | 174, 1583 |
| HpyAV | CCTTC | 7 cut(s) | 17, 470, 1179, 1403, 2029, 2034, 2040 |
| HpyCH4III | ACNGT | 7 cut(s) | 195, 670, 755, 1736, 1996, 2065, 2089 |
| HpyCH4IV | ACGT | 5 cut(s) | 169, 1230, 1365, 1623, 2046 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 388, 626, 692, 1301, 1397, 1419, 1786 |
| HpyF3I | CTNAG | 6 cut(s) | 108, 258, 581, 654, 1209, 1921 |
| HpySE526I | ACGT | 5 cut(s) | 169, 1230, 1365, 1623, 2046 |
| Hsp92I | GRCGYC | 1 cut(s) | 1623 |
| HspAI | GCGC | 2 cut(s) | 623, 2118 |
| KflI | GGGWCCC | 1 cut(s) | 987 |
| Kzo9I | GATC | 8 cut(s) | 210, 300, 521, 723, 741, 1154, 1273, 1973 |
| LguI | GCTCTTC | 1 cut(s) | 432 |
| LmnI | GCTCC | 5 cut(s) | 38, 1301, 1388, 1718, 1837 |
| Lsp1109I | GCAGC | 4 cut(s) | 607, 1570, 1573, 1968 |
| LweI | GCATC | 4 cut(s) | 71, 895, 1166, 1193 |
| MabI | ACCWGGT | 1 cut(s) | 636 |
| MaeI | CTAG | 3 cut(s) | 519, 626, 1374 |
| MaeII | ACGT | 5 cut(s) | 169, 1230, 1365, 1623, 2046 |
| MaeIII | GTNAC | 6 cut(s) | 189, 611, 755, 940, 1213, 1318 |
| MalI | GATC | 8 cut(s) | 212, 302, 523, 725, 743, 1156, 1275, 1975 |
| MbiI | CCGCTC | 1 cut(s) | 287 |
| MboI | GATC | 8 cut(s) | 210, 300, 521, 723, 741, 1154, 1273, 1973 |
| MboII | GAAGA | 7 cut(s) | 97, 272, 349, 449, 713, 1627, 1875 |
| MfeI | CAATTG | 5 cut(s) | 428, 884, 936, 957, 2113 |
| MflI | RGATCY | 3 cut(s) | 300, 521, 1273 |
| MhlI | GDGCHC | 5 cut(s) | 835, 880, 1306, 1566, 1723 |
| MlyI | GAGTC | 6 cut(s) | 232, 244, 851, 1539, 1874, 2060 |
| MmeI | TCCRAC | 3 cut(s) | 774, 956, 1736 |
| Mph1103I | ATGCAT | 1 cut(s) | 1181 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1103, 1309 |
| MspA1I | CMGCKG | 1 cut(s) | 686 |
| MspI | CCGG | 2 cut(s) | 155, 388 |
| MspR9I | CCNGG | 5 cut(s) | 155, 543, 638, 1557, 1728 |
| MunI | CAATTG | 5 cut(s) | 428, 884, 936, 957, 2113 |
| Mva1269I | GAATGC | 1 cut(s) | 427 |
| MvaI | CCWGG | 4 cut(s) | 543, 638, 1557, 1728 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 388, 626, 692, 1301, 1397, 1419, 1786 |
| NciI | CCSGG | 1 cut(s) | 155 |
| NcoI | CCATGG | 1 cut(s) | 696 |
| NdeI | CATATG | 2 cut(s) | 511, 1297 |
| NdeII | GATC | 8 cut(s) | 210, 300, 521, 723, 741, 1154, 1273, 1973 |
| NheI | GCTAGC | 1 cut(s) | 625 |
| NlaIV | GGNNCC | 6 cut(s) | 40, 224, 302, 988, 989, 2016 |
| NmuCI | GTSAC | 2 cut(s) | 755, 940 |
| NsiI | ATGCAT | 1 cut(s) | 1181 |
| NspI | RCATGY | 2 cut(s) | 1195, 1477 |
| OliI | CACNNNNGTG | 1 cut(s) | 1103 |
| PagI | TCATGA | 2 cut(s) | 586, 672 |
| PceI | AGGCCT | 1 cut(s) | 924 |
| PciSI | GCTCTTC | 1 cut(s) | 432 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 1236, 2052 |
| PctI | GAATGC | 1 cut(s) | 427 |
| PfeI | GAWTC | 3 cut(s) | 500, 854, 1183 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1444, 2011 |
| PfoI | TCCNGGA | 1 cut(s) | 1726 |
| PkrI | GCNGC | 6 cut(s) | 517, 622, 688, 1585, 1588, 1958 |
| PleI | GAGTC | 6 cut(s) | 232, 244, 850, 1538, 1874, 2059 |
| PmaCI | CACGTG | 1 cut(s) | 1231 |
| PmlI | CACGTG | 1 cut(s) | 1231 |
| PpsI | GAGTC | 6 cut(s) | 232, 244, 850, 1538, 1874, 2059 |
| Ppu21I | YACGTR | 1 cut(s) | 1231 |
| PpuMI | RGGWCCY | 2 cut(s) | 365, 987 |
| PshAI | GACNNNNGTC | 1 cut(s) | 582 |
| PshBI | ATTAAT | 1 cut(s) | 597 |
| Psp124BI | GAGCTC | 1 cut(s) | 1723 |
| Psp1406I | AACGTT | 1 cut(s) | 2046 |
| Psp5II | RGGWCCY | 2 cut(s) | 365, 987 |
| Psp6I | CCWGG | 4 cut(s) | 541, 636, 1555, 1726 |
| PspCI | CACGTG | 1 cut(s) | 1231 |
| PspGI | CCWGG | 4 cut(s) | 541, 636, 1555, 1726 |
| PspN4I | GGNNCC | 6 cut(s) | 40, 224, 302, 988, 989, 2016 |
| PspPI | GGNCC | 3 cut(s) | 4, 365, 987 |
| PspPPI | RGGWCCY | 2 cut(s) | 365, 987 |
| PsuI | RGATCY | 3 cut(s) | 300, 521, 1273 |
| RsaI | GTAC | 3 cut(s) | 966, 1144, 1761 |
| RsaNI | GTAC | 3 cut(s) | 965, 1143, 1760 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1103, 1309 |
| SacI | GAGCTC | 1 cut(s) | 1723 |
| SapI | GCTCTTC | 1 cut(s) | 432 |
| SatI | GCNGC | 6 cut(s) | 516, 621, 687, 1584, 1587, 1957 |
| Sau3AI | GATC | 8 cut(s) | 210, 300, 521, 723, 741, 1154, 1273, 1973 |
| Sau96I | GGNCC | 3 cut(s) | 4, 365, 987 |
| ScaI | AGTACT | 2 cut(s) | 966, 1144 |
| SchI | GAGTC | 6 cut(s) | 232, 244, 851, 1539, 1874, 2060 |
| ScrFI | CCNGG | 5 cut(s) | 155, 543, 638, 1557, 1728 |
| SduI | GDGCHC | 5 cut(s) | 835, 880, 1306, 1566, 1723 |
| SexAI | ACCWGGT | 1 cut(s) | 636 |
| SfaNI | GCATC | 4 cut(s) | 71, 895, 1166, 1193 |
| SfcI | CTRYAG | 1 cut(s) | 1267 |
| SinI | GGWCC | 3 cut(s) | 4, 365, 987 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1103, 1309 |
| SseBI | AGGCCT | 1 cut(s) | 924 |
| SsiI | CCGC | 8 cut(s) | 97, 287, 485, 516, 686, 721, 1072, 1521 |
| SspMI | CTAG | 3 cut(s) | 519, 626, 1374 |
| SstI | GAGCTC | 1 cut(s) | 1723 |
| StuI | AGGCCT | 1 cut(s) | 924 |
| StyD4I | CCNGG | 5 cut(s) | 153, 541, 636, 1555, 1726 |
| StyI | CCWWGG | 2 cut(s) | 696, 803 |
| TaaI | ACNGT | 7 cut(s) | 195, 670, 755, 1736, 1996, 2065, 2089 |
| TaiI | ACGT | 5 cut(s) | 172, 1233, 1368, 1626, 2049 |
| TaqI | TCGA | 9 cut(s) | 65, 126, 283, 1089, 1239, 1359, 1528, 1839, 1952 |
| TatI | WGTACW | 2 cut(s) | 964, 1142 |
| TauI | GCSGC | 2 cut(s) | 518, 689 |
| TfiI | GAWTC | 3 cut(s) | 500, 854, 1183 |
| TscAI | CASTG | 7 cut(s) | 311, 760, 835, 1047, 1573, 1667, 2001 |
| TseFI | GTSAC | 2 cut(s) | 755, 940 |
| TseI | GCWGC | 4 cut(s) | 620, 1583, 1586, 1956 |
| Tsp45I | GTSAC | 2 cut(s) | 755, 940 |
| TspDTI | ATGAA | 9 cut(s) | 36, 42, 66, 603, 795, 1359, 1442, 1659, 1706 |
| TspGWI | ACGGA | 2 cut(s) | 1695, 2044 |
| TspRI | CASTG | 7 cut(s) | 311, 760, 835, 1047, 1573, 1667, 2001 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1444, 2011 |
| VneI | GTGCAC | 1 cut(s) | 876 |
| VpaK11BI | GGWCC | 3 cut(s) | 4, 365, 987 |
| VspI | ATTAAT | 1 cut(s) | 597 |
| XapI | RAATTY | 4 cut(s) | 67, 590, 714, 1382 |
| XceI | RCATGY | 2 cut(s) | 1195, 1477 |
| XspI | CTAG | 3 cut(s) | 519, 626, 1374 |
| ZraI | GACGTC | 1 cut(s) | 1624 |
| ZrmI | AGTACT | 2 cut(s) | 966, 1144 |
| Zsp2I | ATGCAT | 1 cut(s) | 1181 |