RLG00000032481

F-box-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000620
Physical Location & Seq
Reverse (-)
15726010 .. 15727529
1520 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032481

Sequence Viewer

Length: 1158 bp
ATGCCATCCACAAAGGATTGCTGTGAGTTATCTGGTCACTTCCACAATGTAAATCTGAGACAAAACCCGGCGGGGACAGTTGAGACTCAAGCTAGGGCACTTTCAATAAAGAGAAAGAAGGTGTCTGATGCAGCACCAGAGCTTCCTTTTGATATGATCACTGAGATTTTGAGCTGGTTGCCTGTAGATTCTTTGCTAAGGTTCAAGTGTGTGTGTAAAAAATGGCGTTCCTTGCTTCTAGACTACAATTTCATTGCAAAACATATGGTTCGAGCCAGTCCTCTGCAACTTTCCCATAAAAAAGAATGGGAACCTAACAAATTGATCTGGACCATTCTTTCTGAGGCAAACTTCAAGCTTATCTCAGATGTGGCTGGCTTGCTTCTGGAGGAGAGTCTTACTTCTCGAGTTTCCCGAATCAGAAACTTTGCAACGCATCAAGTACTTTACTTGCCTGATGCACATGGGAAAACTAGTACAGTGGGTTTTGTTTTTGATTCATCCACTTGTGAGTGTAAAGCGGCATGTTTTCATTGGAAACAGGAGGGTGACACTGGCTATGAAGTAGGCTTTAAAGTTCTAAGTATCGGAAAGGATGATCAATGGAGGACCCTGAAGCTGCCCAAGCAAAATGGCAAATTGTTCGTGGCACGATATTTTATAGCAACTAACGAAGAAGGGGCCGCTCATGCGGTGGAAATTATTAGAGATGGACAAGATTTCAAGCTAGAAGTTCAATCTGTTGATATATGGAGTGAATGTTTCACCATTACCACTCTGCCCCGGGGAGCTTTCTTAGACTTGAAAAGAGTTGCAATTTTCCGTTGGAATTACTATGTAGCTGTTGCTGATATAGTAGAGGAATCCCTTAACGTCTTGGTGTTGGAAGACTTCAAAAAACACAAATGGAGGAAGATCATTGTTCCCTTGAAATTCTTGAAGGACAATCCAGGTTTAAAAGATGAGATTCGCCCTCATCAAGTTTGGTTAAATTACCTCCGGTTGCACAATGCAGTGAAAAAGAATATTCTCGTGTATGATATGGAAAGGAAGGTGATCAAGGTGATCAACGTGACGCATACCAAGTCTACGGAGAAAATATATGAATTCCGAAAGCCAAGCCTGGTTACTCTCAAGGGAATGAAGAATGAATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_3

Protein Analysis

386

Amino Acids

44.48

Weight (kDa)

9.23

Isoelectric Point (pI)

34.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 47 - 84 2.4e-10 F-box domain
F-box-like PF12937 47 - 81 2.7e-07 F-box-like
FBA_3 PF08268 117 - 364 2.1e-10 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000620)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03910 FvH4_4g13750 FvH4_6g21492 FvH4_6g21740 FvH4_6g24450
malus_domestica MD05G1336000.v1.1 MD05G1336100.v1.1 MD10G1212400.v1.1 MD10G1311600.v1.1
prunus_persica Prupe.4G112200_v2.0.a1 Prupe.4G130000_v2.0.a1 Prupe.4G130300_v2.0.a1 Prupe.4G219800_v2.0.a1 Prupe.4G219900_v2.0.a1 Prupe.4G220100_v2.0.a1
pyrus_communis pycom05g30760 pycom10g26280
rosa_chinensis RchiOBHm_Chr3g0478691 RchiOBHm_Chr3g0479021 RchiOBHm_Chr5g0004511 RchiOBHm_Chr5g0019881 RchiOBHm_Chr5g0021181
rosa_laevigata RLG00000023610 RLG00000023612 RLG00000031235 RLG00000032481 RLG00000032506 RLG00000032589 RLG00000032592
rosa_multiflora Rmu_co8446543.1_g000001 Rmu_sc0001396.1_g000010 Rmu_sc0002454.1_g000005 Rmu_sc0002531.1_g000072 Rmu_sc0002715.1_g000028 Rmu_sc0005207.1_g000008 Rmu_sc0005645.1_g000010 Rmu_sc0008074.1_g000017 Rmu_sc0012286.1_g000001 Rmu_ssc0000244.1_g000041
rosa_roxburghii Rroxscaffold_1G00039650 Rroxscaffold_1G00058030 Rroxscaffold_1G00058290 Rroxscaffold_1G00071010 Rroxscaffold_6G00402820 Rroxscaffold_6G00403180
rosa_rugosa Rorug03G0167000 Rorug03G0167100 Rorug03G0170200 Rorug03G0304300 Rorug05G0054800 Rorug05G0065000 Rorug05G0189900
rosa_samantha Rh3AG219300 Rh3AG221100 Rh3BG251000 Rh3BG253400 Rh3BG253800 Rh3CG247800 Rh3CG250000 Rh3DG244100 Rh3DG245900 Rh4AG008500 Rh4CG009200 Rh4CG009600 Rh5AG275400 Rh5BG144100 Rh5BG144400 Rh5BG144500 Rh5BG147400 Rh5BG280200 Rh5CG043100 Rh5CG155800 Rh5CG312400 Rh5DG143800 Rh5DG146700 Rh5DG288400
rosa_wichuraiana Rw3G019900 Rw5G012800 Rw5G013070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 686
AccI GTMKAC 1 cut(s) 1088
AciI CCGC 4 cut(s) 71, 521, 684, 692
AcsI RAATTY 2 cut(s) 932, 1106
AcuI CTGAAG 1 cut(s) 635
AfaI GTAC 2 cut(s) 444, 478
AgsI TTSAA 9 cut(s) 105, 205, 355, 724, 737, 805, 895, 931, 940
AhlI ACTAGT 1 cut(s) 473
AjnI CCWGG 2 cut(s) 949, 1122
AluBI AGCT 8 cut(s) 92, 142, 174, 358, 619, 727, 791, 842
AluI AGCT 8 cut(s) 92, 142, 174, 358, 619, 727, 791, 842
Alw26I GTCTC 2 cut(s) 52, 77
Ama87I CYCGRG 2 cut(s) 405, 783
AoxI GGCC 1 cut(s) 681
ApeKI GCWGC 2 cut(s) 131, 619
ApoI RAATTY 2 cut(s) 932, 1106
AspS9I GGNCC 3 cut(s) 330, 609, 681
AsuC2I CCSGG 3 cut(s) 68, 784, 785
AsuHPI GGTGA 4 cut(s) 560, 757, 1066, 1075
AvaI CYCGRG 2 cut(s) 405, 783
AvaII GGWCC 2 cut(s) 330, 609
BaeGI GKGCMC 1 cut(s) 100
BarI GAAGNNNNNNTAC 1 cut(s) 1136
BauI CACGAG 1 cut(s) 1031
BbsI GAAGAC 1 cut(s) 894
BbvI GCAGC 2 cut(s) 143, 606
BccI CCATC 2 cut(s) 13, 704
BcgI CGANNNNNNTGC 2 cut(s) 625, 659
BciT130I CCWGG 2 cut(s) 951, 1124
BclI TGATCA 4 cut(s) 156, 598, 1056, 1065
BcnI CCSGG 3 cut(s) 68, 784, 785
BcoDI GTCTC 2 cut(s) 52, 77
BcuI ACTAGT 1 cut(s) 473
BfaI CTAG 5 cut(s) 93, 239, 474, 728, 1156
BfmI CTRYAG 1 cut(s) 183
BisI GCNGC 4 cut(s) 132, 522, 620, 684
BlsI GCNGC 4 cut(s) 133, 523, 621, 685
BmcAI AGTACT 1 cut(s) 444
Bme1390I CCNGG 5 cut(s) 68, 784, 785, 951, 1124
Bme18I GGWCC 2 cut(s) 330, 609
BmeT110I CYCGRG 2 cut(s) 405, 783
BmgT120I GGNCC 3 cut(s) 330, 609, 681
BmiI GGNNCC 3 cut(s) 312, 611, 682
BmrFI CCNGG 5 cut(s) 68, 784, 785, 951, 1124
BmsI GCATC 3 cut(s) 118, 445, 448
BpiI GAAGAC 1 cut(s) 894
BpmI CTGGAG 1 cut(s) 407
Bpu10I CCTNAGC 1 cut(s) 197
BpuEI CTTGAG 2 cut(s) 72, 1118
BpuMI CCSGG 3 cut(s) 68, 784, 785
BsaJI CCNNGG 3 cut(s) 782, 783, 784
BsaWI WCCGGW 1 cut(s) 999
BsaXI ACNNNNNCTCC 2 cut(s) 745, 775
Bse1I ACTGG 2 cut(s) 276, 559
Bse3DI GCAATG 1 cut(s) 252
BseBI CCWGG 2 cut(s) 951, 1124
BseDI CCNNGG 3 cut(s) 782, 783, 784
BseGI GGATG 3 cut(s) 5, 500, 601
BseMI GCAATG 1 cut(s) 252
BseMII CTCAG 4 cut(s) 47, 153, 333, 378
BseNI ACTGG 2 cut(s) 276, 559
BseRI GAGGAG 1 cut(s) 404
BseSI GKGCMC 1 cut(s) 100
BseXI GCAGC 2 cut(s) 143, 606
BshFI GGCC 1 cut(s) 683
BsiHKCI CYCGRG 2 cut(s) 405, 783
BsiSI CCGG 3 cut(s) 68, 784, 1000
BslFI GGGAC 1 cut(s) 88
BsmAI GTCTC 2 cut(s) 52, 77
BsmFI GGGAC 1 cut(s) 88
BsnI GGCC 1 cut(s) 683
BsoBI CYCGRG 2 cut(s) 405, 783
Bsp1286I GDGCHC 1 cut(s) 100
Bsp143I GATC 6 cut(s) 156, 324, 598, 915, 1056, 1065
BspACI CCGC 4 cut(s) 71, 521, 684, 692
BspANI GGCC 1 cut(s) 683
BspCNI CTCAG 4 cut(s) 48, 154, 334, 377
BspLI GGNNCC 3 cut(s) 312, 611, 682
BsrBI CCGCTC 1 cut(s) 686
BsrDI GCAATG 1 cut(s) 252
BsrI ACTGG 2 cut(s) 276, 559
BssECI CCNNGG 3 cut(s) 782, 783, 784
BssMI GATC 6 cut(s) 156, 324, 598, 915, 1056, 1065
BssSI CACGAG 1 cut(s) 1031
Bst2BI CACGAG 1 cut(s) 1031
Bst2UI CCWGG 2 cut(s) 951, 1124
Bst4CI ACNGT 2 cut(s) 79, 481
BstC8I GCNNGC 2 cut(s) 376, 380
BstDEI CTNAG 7 cut(s) 56, 162, 197, 342, 364, 581, 796
BstF5I GGATG 3 cut(s) 5, 500, 601
BstKTI GATC 6 cut(s) 159, 327, 601, 918, 1059, 1068
BstMAI GTCTC 2 cut(s) 52, 77
BstMBI GATC 6 cut(s) 156, 324, 598, 915, 1056, 1065
BstMWI GCNNNNNNNGC 3 cut(s) 232, 625, 689
BstNI CCWGG 2 cut(s) 951, 1124
BstNSI RCATGY 1 cut(s) 528
BstSCI CCNGG 5 cut(s) 66, 782, 783, 949, 1122
BstSFI CTRYAG 1 cut(s) 183
BstSLI GKGCMC 1 cut(s) 100
BstV1I GCAGC 2 cut(s) 143, 606
BstV2I GAAGAC 1 cut(s) 894
BsuRI GGCC 1 cut(s) 683
BtsCI GGATG 3 cut(s) 5, 500, 601
BtsI GCAGTG 1 cut(s) 1020
BtsIMutI CAGTG 4 cut(s) 159, 486, 552, 1020
Cac8I GCNNGC 2 cut(s) 376, 380
Cfr13I GGNCC 3 cut(s) 330, 609, 681
Cfr9I CCCGGG 1 cut(s) 783
CseI GACGC 1 cut(s) 1084
Csp6I GTAC 2 cut(s) 443, 477
CviAII CATG 3 cut(s) 464, 525, 689
CviQI GTAC 2 cut(s) 443, 477
DdeI CTNAG 7 cut(s) 56, 162, 197, 342, 364, 581, 796
DpnI GATC 6 cut(s) 158, 326, 600, 917, 1058, 1067
DpnII GATC 6 cut(s) 156, 324, 598, 915, 1056, 1065
DraI TTTAAA 2 cut(s) 574, 957
Eco47I GGWCC 2 cut(s) 330, 609
Eco57I CTGAAG 1 cut(s) 635
Eco88I CYCGRG 2 cut(s) 405, 783
EcoO109I RGGNCCY 1 cut(s) 609
EcoRI GAATTC 1 cut(s) 1106
EcoRII CCWGG 2 cut(s) 949, 1122
FaeI CATG 3 cut(s) 467, 528, 692
FaqI GGGAC 1 cut(s) 88
FatI CATG 3 cut(s) 463, 524, 688
FauI CCCGC 1 cut(s) 64
FauNDI CATATG 1 cut(s) 264
FbaI TGATCA 4 cut(s) 156, 598, 1056, 1065
FblI GTMKAC 1 cut(s) 1088
Fnu4HI GCNGC 4 cut(s) 132, 522, 620, 684
FokI GGATG 2 cut(s) 487, 608
Fsp4HI GCNGC 4 cut(s) 132, 522, 620, 684
FspBI CTAG 5 cut(s) 93, 239, 474, 728, 1156
GluI GCNGC 4 cut(s) 132, 522, 620, 684
GsuI CTGGAG 1 cut(s) 407
HaeIII GGCC 1 cut(s) 683
HapII CCGG 3 cut(s) 68, 784, 1000
HgaI GACGC 1 cut(s) 1084
Hin1II CATG 3 cut(s) 467, 528, 692
HindIII AAGCTT 1 cut(s) 356
HinfI GANTC 7 cut(s) 85, 188, 394, 417, 497, 863, 967
HpaII CCGG 3 cut(s) 68, 784, 1000
HphI GGTGA 4 cut(s) 560, 757, 1066, 1075
Hpy166II GTNNAC 1 cut(s) 1089
Hpy188I TCNGA 7 cut(s) 57, 127, 343, 367, 422, 590, 1112
Hpy188III TCNNGA 6 cut(s) 239, 328, 386, 405, 414, 937
Hpy8I GTNNAC 1 cut(s) 1089
HpyAV CCTTC 4 cut(s) 112, 671, 934, 1045
HpyCH4III ACNGT 2 cut(s) 79, 481
HpyCH4IV ACGT 2 cut(s) 873, 1071
HpyCH4V TGCA 8 cut(s) 131, 257, 286, 431, 461, 815, 1006, 1013
HpyF10VI GCNNNNNNNGC 3 cut(s) 232, 625, 689
HpyF3I CTNAG 7 cut(s) 56, 162, 197, 342, 364, 581, 796
HpySE526I ACGT 2 cut(s) 873, 1071
Hsp92II CATG 3 cut(s) 467, 528, 692
Ksp22I TGATCA 4 cut(s) 156, 598, 1056, 1065
Kzo9I GATC 6 cut(s) 156, 324, 598, 915, 1056, 1065
LmnI GCTCC 1 cut(s) 788
Lsp1109I GCAGC 2 cut(s) 143, 606
LweI GCATC 3 cut(s) 118, 445, 448
MaeI CTAG 5 cut(s) 93, 239, 474, 728, 1156
MaeII ACGT 2 cut(s) 873, 1071
MaeIII GTNAC 4 cut(s) 35, 548, 1072, 1126
MalI GATC 6 cut(s) 158, 326, 600, 917, 1058, 1067
MbiI CCGCTC 1 cut(s) 686
MboI GATC 6 cut(s) 156, 324, 598, 915, 1056, 1065
MboII GAAGA 4 cut(s) 686, 899, 925, 1156
MhlI GDGCHC 1 cut(s) 100
MluCI AATT 9 cut(s) 247, 320, 638, 699, 816, 829, 932, 991, 1106
MlyI GAGTC 2 cut(s) 79, 403
MmeI TCCRAC 2 cut(s) 806, 864
MnlI CCTC 9 cut(s) 291, 337, 382, 538, 600, 853, 903, 984, 1007
MseI TTAA 4 cut(s) 573, 870, 956, 989
MspI CCGG 3 cut(s) 68, 784, 1000
MspR9I CCNGG 5 cut(s) 68, 784, 785, 951, 1124
MvaI CCWGG 2 cut(s) 951, 1124
MwoI GCNNNNNNNGC 3 cut(s) 232, 625, 689
NciI CCSGG 3 cut(s) 68, 784, 785
NdeI CATATG 1 cut(s) 264
NdeII GATC 6 cut(s) 156, 324, 598, 915, 1056, 1065
NlaIII CATG 3 cut(s) 467, 528, 692
NlaIV GGNNCC 3 cut(s) 312, 611, 682
NmuCI GTSAC 3 cut(s) 35, 548, 1072
NspI RCATGY 1 cut(s) 528
PaeR7I CTCGAG 1 cut(s) 405
PcsI WCGNNNNNNNCGW 1 cut(s) 412
PfeI GAWTC 5 cut(s) 188, 417, 497, 863, 967
PkrI GCNGC 4 cut(s) 133, 523, 621, 685
PleI GAGTC 2 cut(s) 79, 402
PpsI GAGTC 2 cut(s) 79, 402
PpuMI RGGWCCY 1 cut(s) 609
Psp5II RGGWCCY 1 cut(s) 609
Psp6I CCWGG 2 cut(s) 949, 1122
PspGI CCWGG 2 cut(s) 949, 1122
PspN4I GGNNCC 3 cut(s) 312, 611, 682
PspPI GGNCC 3 cut(s) 330, 609, 681
PspPPI RGGWCCY 1 cut(s) 609
RsaI GTAC 2 cut(s) 444, 478
RsaNI GTAC 2 cut(s) 443, 477
SaqAI TTAA 4 cut(s) 573, 870, 956, 989
SatI GCNGC 4 cut(s) 132, 522, 620, 684
Sau3AI GATC 6 cut(s) 156, 324, 598, 915, 1056, 1065
Sau96I GGNCC 3 cut(s) 330, 609, 681
ScaI AGTACT 1 cut(s) 444
SchI GAGTC 2 cut(s) 79, 403
ScrFI CCNGG 5 cut(s) 68, 784, 785, 951, 1124
SduI GDGCHC 1 cut(s) 100
SfaNI GCATC 3 cut(s) 118, 445, 448
SfcI CTRYAG 1 cut(s) 183
Sfr274I CTCGAG 1 cut(s) 405
SinI GGWCC 2 cut(s) 330, 609
SlaI CTCGAG 1 cut(s) 405
SmaI CCCGGG 1 cut(s) 785
SmlI CTYRAG 3 cut(s) 87, 405, 1133
SmoI CTYRAG 3 cut(s) 87, 405, 1133
SpeI ACTAGT 1 cut(s) 473
Sse9I AATT 9 cut(s) 247, 320, 638, 699, 816, 829, 932, 991, 1106
SsiI CCGC 4 cut(s) 71, 521, 684, 692
SspI AATATT 1 cut(s) 1027
SspMI CTAG 5 cut(s) 93, 239, 474, 728, 1156
StyD4I CCNGG 5 cut(s) 66, 782, 783, 949, 1122
TaaI ACNGT 2 cut(s) 79, 481
TaiI ACGT 2 cut(s) 876, 1074
TaqI TCGA 2 cut(s) 271, 406
TasI AATT 9 cut(s) 247, 320, 638, 699, 816, 829, 932, 991, 1106
TatI WGTACW 2 cut(s) 442, 476
TauI GCSGC 2 cut(s) 524, 686
TfiI GAWTC 5 cut(s) 188, 417, 497, 863, 967
Tru1I TTAA 4 cut(s) 573, 870, 956, 989
Tru9I TTAA 4 cut(s) 573, 870, 956, 989
TscAI CASTG 4 cut(s) 166, 486, 559, 1020
TseFI GTSAC 3 cut(s) 35, 548, 1072
TseI GCWGC 2 cut(s) 131, 619
Tsp45I GTSAC 3 cut(s) 35, 548, 1072
TspDTI ATGAA 6 cut(s) 241, 489, 521, 576, 1119, 1157
TspGWI ACGGA 2 cut(s) 812, 1106
TspMI CCCGGG 1 cut(s) 783
TspRI CASTG 4 cut(s) 166, 486, 559, 1020
VpaK11BI GGWCC 2 cut(s) 330, 609
XapI RAATTY 2 cut(s) 932, 1106
XbaI TCTAGA 1 cut(s) 238
XceI RCATGY 1 cut(s) 528
XhoI CTCGAG 1 cut(s) 405
XmaI CCCGGG 1 cut(s) 783
XmiI GTMKAC 1 cut(s) 1088
XspI CTAG 5 cut(s) 93, 239, 474, 728, 1156
ZrmI AGTACT 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.